Oncotarget
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2017
·
IF 5.168
Pengfei Chen,Sheng Mei,Chen Xia,Ren Zhu,Yichuan Pang,Jiying Wang,Jianfeng Zhang,Fangchun Shao,Shunwu Fan
The present study aimed to investigate the synergistic therapeutic effect of decreasing cartilage angiogenesis via exposure to crizotinib encapsulated by chitosan microspheres and photo-crosslinked hydrogel, with the goal of evaluating crizotinib as a treatment for osteoarthritis. First, we developed and evaluated the characteristics of hydrogels and chitosan microspheres. Next, we measured the effect of crizotinib on the cartilage degeneration induced by interleukin-1β in chondrocytes. Crizotinib ameliorated the pathological changes induced by interleukin-1β via its anti-angiogenesis function. In addition, we surgically induced osteoarthritis in mice, which were then injected intra-articularly with crizotinib-loaded biomaterials. Cartilage matrix degradation, expression of vascular endothelial growth factor and extracellular signal-regulated kinases 1/2 were evaluated after surgery. Treatment with the combination of crizotinib-loaded biomaterials retarded the progression of surgically induced osteoarthritis. Crizotinib ameliorated cartilage matrix degradation by promoting anti-angiogenesis and impeding extracellular signal-regulated kinases 1/2 signaling pathway. Our results demonstrate that the combination of photo-crosslinked hydrogel and crizotinib-loaded chitosan microspheres might represent a promising strategy for osteoarthritis treatment.
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CARBOHYDRATE POLYMERS
·
2017
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IF 4.811
Hui Guo,Lu Xue,Shen Yao,Xiaohua Cai,Junqing Qian
A new solid-phase extraction mode was developed for separation some flavones from the complex interference system. In the experiment, rhein anchored on magnetic chitosan microparticles, prepared facilely without removal of the template molecule, was used as sorbents for the extraction of target analytes; after completion of the extraction process, Fe 3 O 4 particles acted as carrier to retrieve rhein functionalized magnetic chitosan microparticles (RMCMPs) from the sample solution. RMCMPs showed excellent selective adsorption capacity of isoflavones. The result suggested that the method was useful for fast determination and quantification of isoflavones in soymilk. The RMCMPs were characterized by scanning electron microscope (SEM), X-ray diffraction (XRD) and Fourier transforms infrared spectrometer (FTIR).
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NEW JOURNAL OF CHEMISTRY
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2017
·Vol. 41
·期 16
·8156-8165
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IF 3.269
Linjun Shao,Chenze Qi
Chitosan microspheres-supported palladium catalysts (Pd@CM) for Mizoroki–Heck reaction have been successfully prepared by electrospraying PdCl2/chitosan mixture in a trifluoroacetic acid (TFA) aqueous solution. Swelling capacity measurement and thermal analysis demonstrated that the palladium species in the chitosan microspheres could cross-link the chitosan efficiently. FT-IR spectra demonstrated that the chitosan in the microspheres could be cross-linked by glutaraldehyde, and the formed Schiff bond could chelate the palladium species efficiently. High resolution transmission electron microscopy (HR-TEM) indicated that the entrapment of palladium species in chitosan microspheres could enhance its dispersion. Mizoroki–Heck reaction catalysis results demonstrated that the catalytic activity of Pd@CM was associated with the size of chitosan microspheres. The Pd@CM with a suitable size was even more active than the catalyst with palladium species adsorbed on the surface of chitosan microspheres (Pd-CM). Moreover, the entrapment of palladium species in chitosan microspheres could reduce its aggregation and leaching in the catalysis procedure, which in turn increased the reusability of Pd@CM catalyst. The high catalytic activity and stability of Pd@CM have been attributed to the entrapment of palladium species within the cross-linked chitosan microspheres and the chelation of palladium species with the abundant amine and hydroxyl groups on chitosan chain.
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ENVIRONMENTAL SCIENCE AND POLLUTION RESEARCH
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2017
·Vol. 24
·期 22
·18640-18650
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IF 2.741
Chen Yuan,Lei Xin,Dou Rongni,Chen Yuancai,Hu Yongyou,Zhang Zhiqi
A water-compatible magnetic triclosan (TCS) imprinted material (TCS-CTS-Fe 0 -MIPs) was synthesized for selective enrichment and detection of TCS in real complex water samples. The material was synthesized by using chitosan (CTS) as functional monomer, which has rich surface O- and N-containing functional groups. The TCS imprinted CTS was coated on Fe 0 surface and then cross-linked with glutaraldehyde. Scanning electron microscopy suggested that the imprinted material was covered with a layer of imprinted film, Fourier transform infrared spectroscopy and X-ray photoelectron spectroscopy confirmed that the imprinted material had more functional groups (amino and hydroxyl groups) than that of non-imprinted material. The TCS imprinted and non-imprinted materials used in each adsorption experiments were 0.1 mg mL −1 . The maximum adsorption capacity of the TCS imprinted material and non-TCS imprinted material were 20.86 and 15.11 mg g −1 , respectively. The adsorption results showed that selectivity coefficient was 10.151, 1.353, and 8.271 in the presence of p-chlorophenol, 2,4,6-trichlorophenol, and bisphenol-A, respectively. The recoveries of river water and lake water samples were 92.8, 91.3, 92.4, and 81.4, 82.3, 82.1%, respectively, when the samples were spiked with 4, 6, and 8 μg L −1 of TCS with the imprinted material. The adsorption capacity of the TCS imprinted material and non-TCS imprinted material lost 5.2 and 6.2% after six times of recycling. The high selectivity and excellent adsorption capacity of the imprinted material can be attributed to the presence of sterically complementary imprinted sites and high surface, which would also made it more accessible to TCS than that of non-imprinted material. The present study would provide an environmental friendly and convenient method for the removal and the monitoring of TCS in environmental water samples.
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JOURNAL OF MATERIALS SCIENCE & TECHNOLOGY
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2017
·
IF 2.764
Haoxiang Zhong,Jidian Lu,Aiqin He,Minghao Sun,Jiarong He,Lingzhi Zhang
Carboxymethyl chitosan/poly (ethylene oxide) (CCTS/PEO) composite is firstly reported as a water soluble binder for the application of 5 V LiNi 0.5 Mn 1.5 O 4 cathode in Li-ion batteries. Both CCTS and PEO show a high electrochemical oxidation potential of above 5.0 V (vs. Li/Li + ). The electrochemical performances of LiNi 0.5 Mn 1.5 O 4 (LNMO) cathodes with CCTS/PEO composite binders of different mass rates are investigated, it is found that LiNi 0.5 Mn 1.5 O 4 cathode with an optimized CCTS/PEO (85/15, w/w) composite exhibits a slightly better cycling performance than that of polyvinylidene fluoride (PVDF), retaining 81.4% capacity as compared with 79.8% for PVDF at 0.5C rate after 200 cycles. LNMO with PEO/CCTS (85/15, w/w) exhibited the better rate capability than that of PVDF. These results demonstrate that CCTS/PEO composite can be potentially used as a water-soluble binder for 5 V LNMO cathode.
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CARBOHYDRATE POLYMERS
·
2016
·
IF 4.219
Juan Du,Yuqi Zhou,Lulu Wang,Yancai Wang
Here a series of multifunctional stabilizers was designed and used in a nanosuspension stability enhancement study. Methoxypolyethylene glycol (M PEG)–grafted chitosan, accompanied by space steric hindrance, an electrostatic repulsion function, and a solvation effect, is a multifunctional stabilizer. Deacetyl mycoepoxydience (DM) nanosuspension was prepared using the anti-solvent precipitation approach. The effects of the DM and the multifunctional stabilizer concentration, solvent to anti-solvent ratio, crystallization and storage temperature, and ultrasonic time on drug particle formation during the anti-solvent processing were investigated and the nanosuspension stability was studied. The nanosuspension showed dendritic-like nanostructures and a crystalline state in a morphology and crystalline state study. The optimized drug and multifunctional stabilizer concentration range were selected through the response surface optimization method. The most appropriate and stable nanosuspension could be obtained through the optimal parameters. This study demonstrated that M PEG–grafted chitosan (M PEGC) could be used as a multifunctional stabilizer to control particle size and improve nanosuspension stability.
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JOURNAL OF BIOACTIVE AND COMPATIBLE POLYMERS
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2017
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IF 1.31
Yang Liu,Zhongxun Zhang,Huilin Lv,Yong Qin,Linhong Deng
Chitosan-based material has been widely used as bone substitute due to its good biocompatibility and biodegradability. However, the hydrophobic surface of chitosan film constrains the osteogenesis mineralization in the process of bone regeneration. For this reason, we develop a novel polydopamine-modified chitosan film suitable for bone tissue engineering applications by a simple and feasible route in this study. Fourier transform infrared spectroscopy and X-ray photoelectron spectroscopy confirm the process of surface modification. For comparison, surface wettability, the capacity of mineralization in vitro, and biocompatibility of the chitosan film and the polydopamine-modified chitosan film were assessed. Research results indicate that the polydopamine-modified chitosan film has good hydrophilicity. It is very evident that the polydopamine treatment significantly influences the biomineralization capacity of the chitosan-based substrates, which enhance the growth rate of apatite on the modified chitosan film. Besides, MC3T3-E1 osteoblast experiments demonstrate that the cells can adhere and grow well on the polydopamine-modified chitosan film. It is anticipated that this polydopamine-modified chitosan film, which can be prepared in large quantities simply, should have potential applications in bone tissue engineering.
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CARBOHYDRATE POLYMERS
·
2016
·
IF 4.219
Dong-Yan Wu,Yu Ma,Xiao-Shuang Hou,Wen-Jie Zhang,Pei Wang,Huan Chen,Bo Li,Can Zhang,Ya Ding
Although combination delivery (co-delivery) shows much superiority in the defect compensation of single-agent therapy, the construction and application of co-delivery systems are still challenging, especially for protein-based joint systems. In this work, a series of chitosan (CS)-amino acid derivatives (Arg-CS, Lys-CS, and Phe-CS) with different degrees of substitution (DS) were synthesized to prepare CS nanocapsules (CNCs) using a simple emulsification method in the presence of linoleic acid (LA). The hydrophobic drug can be loaded in LA droplets, and a positively charged protein stabilized the optimized Arg-CS nanocapsules (Arg-CNCs) on their negative surfaces. The in vitro antitumor efficacy of Arg-CNCs co-delivering paclitaxel and recombinant human caspase-3 was evaluated in HeLa cells. The co-delivery system displayed much lower IC 50 values and a higher percentage of apoptotic cells compared with the control groups. This system provides a promising and universal strategy for co-delivery, leading to collaborative tumor treatment.
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BIOMATERIALS
·
2016
·
IF 8.387
Gao-Na Shi,Chuang-Nian Zhang,Rong Xu,Jin-Feng Niu,Hui-Juan Song,Xiu-Yuan Zhang,Wei-Wei Wang,Yan-Ming Wang,Chen Li,Xiao-Qing Wei,De-Ling Kong
Whole tumor cell lysates (TCL) have been implemented as tumor antigens for cancer vaccine development, although clinical outcomes of TCL-based antitumor immunotherapy remain unsatisfactory. In order to improve the efficacy of TCL-based vaccines, biomaterials have been employed to enhance antigen delivery and presentation. Here, we have developed chitosan nanoparticles (CTS NPs) with surface mannose (Man) moieties for specific dendritic cells (DCs) targeting (Man-CTS NPs). The Man-CTS NPs were then loaded with TCL generated from B16 melanoma cells (Man-CTS-TCL NPs) for in vitro and in vivo assessment. Potency of the Man-CTS-TCL NPs as cancer vaccine was also assessed in vivo by immunization of mice with Man-CTS-TCL NPs followed by re-challenge with B16 melanoma cell inoculation. We have shown here that Man-CTS-TCL NPs promote bone marrow-derived dendritic cells (BMDCs) maturation and antigen presentation in vitro . In vivo evaluation further demonstrated that the Man-CTS-TCL NPs were readily taken up by endogenous DCs within the draining lymph node (DLN) following subcutaneous administration accompanied by increasing in serum IFN-γ and IL-4 levels. Tumor growth was also significantly delayed in mice primed with Man-CTS-TCL NPs vaccine, attributable at least in part to cytotoxic T lymphocytes response. Moreover, Man-CTS-TCL NPs vaccine also exhibited therapeutic effects in mice with melanoma. Thus, we report here the Man-CTS-TCL NPs as effective anti-tumor vaccine for cancer immunotherapy .
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Biomaterials Advances
·
2024
·
IF 5.5
Kunpeng Jia,Changpeng Zuo,Yan Xu,Wenfu Ma,Lingtao Wang,Yan Ji,Jie Chen,Qiuyang Zhang,Changjiang Pan,Tingting Liu
Titanium and its alloys are widely used as orthopedic implants owing to their good mechanical properties and excellent corrosion resistance. However, the insufficient osteogenic activity and antibacterial properties hinder their clinical applications. To address these issues, TiO 2 nanotube arrays (TNT) were first fabricated on the TA2 alloy surface via an anodizing technique, and strontium ions (Sr 2+ ) were then loaded by hydrothermal reaction (TNT + Sr) and annealing treatment (TNT + A). Subsequently, the polydopamine layer (TNT + PDA) was constructed to immobilize the carboxymethyl chitosan and alendronate sodium (TNT + CA) mixture. The prepared coatings were thoroughly characterized by scanning electron microscopy (SEM), energy dispersive spectrometer (EDS), X-ray photoelectron spectrometer (XPS), attenuated total reflection-Fourier transform infrared spectroscopy (ATR-FTIR), X-ray diffractometer (XRD), and water contact angle measurement. The results confirmed that Sr 2+ ions, polydopamine, and carboxymethyl chitosan/alendronate sodium were successfully immobilized on the nanotubes. The coating of TNT + CA significantly enhanced the hydrophilicity, and effectively delayed the release of Sr 2+ and alendronate. The TNT + CA coating significantly promoted osteoblast adhesion and proliferation, and up-regulated the expressions of alkaline phosphatase (ALP), osteocalcin (OCN), and osteoblast-specific transcription factor (RUNX2). TNT + CA was able to rapidly induce in situ hydroxyapatite deposition from the simulated body fluid (SBF). Moreover, TNT + CA coating showed inhibition against Escherichia coli and Staphylococcus aureus (especially against Escherichia coli ). The prepared TNT + CA coating provides a novel strategy for enhancing bone affinity, improving osteoblast behaviors, and antibacterial properties of titanium-based biomaterials.
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ACS Applied Materials & Interfaces
·
2024
·
IF 8.3
Zeqi Hao,Jiakun Wang,Linan Jiang,Yinghong Wang,Ling Shen,Jiacheng Liu,Hui Zhang
The accelerated deterioration of carbonate stone artifacts under climate change has long been an urgent issue. Inspired by biomineralization, we developed carboxymethyl chitosan-diammonium hydrogen phosphate (CD) composite and investigated the conservation effectiveness of the CD composite compared to diammonium hydrogen phosphate (DAP) on limestone. The morphologies and microstructures were characterized using scanning electron microscopy (SEM), X-ray diffraction (XRD), and Fourier-transform infrared spectroscopy (FT-IR). The consolidating effectiveness was investigated through a compressive strength test. The protective ability was assessed by disintegration resistance test, acid attack resistance test, salt attack resistance test, and freeze–thaw aging cycle test. SEM observations revealed that carboxymethyl chitosan (CMCS) served as an effective template, inducing the in situ formation of a uniform and continuous calcium phosphate coating on both the surface and interior of the stone. The tests indicated that the CD composite further enhanced the consolidating effectiveness and improved resistance to disintegration and freeze–thaw cycles. Notably, as an amphiphilic polyelectrolyte, CMCS functioned as a pH buffer and a protective barrier against sodium sulfate salts, which improved the resistance to acid and salt attacks. Additionally, the CD composite did not cause significant variations in the esthetic appearance or water vapor permeability. We then applied the CD composite at an actual carbonate stone cultural heritage site, successfully demonstrating the feasibility of CD application and the reversibility of CMCS in a real-world setting. Based on the study’s results, our approach provides a new perspective for developing multifunctional and sustainable conservation materials for carbonate stone artifacts.
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INTERNATIONAL JOURNAL OF BIOLOGICAL MACROMOLECULES
·
2025
·
IF 8.5
Zhe Liu,Lei Chen,Xiaoliang Zhang,Xiaojie Lu,Mao Peng,Chunxia Wang,Yong Liu,Xiangwu Zhang
Flexible Zn-air batteries (FZABs) are regarded as promising energy storage devices for wearable electronics because of their intrinsic safety and high energy densities. However, the low water absorption, low ionic conductivity, and poor water retention of hydrogel electrolytes lead to serious charge/discharge polarization, low discharge capacity, and a short lifespan of FZABs. A novel hydrogel, named polyvinyl alcohol-polyacrylamide-carboxymethyl chitosan (PVA-PAM-CMCS), is developed to address the aforementioned issues. Through the hydrogen bonds and electrostatic interactions, CMCS is connected with the PAM-PVA backbone, which induces a denser and finer porous structure in the hydrogel. Taking advantage of the structural modification, the PVA-PAM-CMCS hydrogel electrolyte shows enhanced ionic conductivity (108.65 mS cm −1 ) and improved tensile stress (244.1 kPa) and strain (410.7 %). 2D fabric FZABs with PVA-PAM-CMCS hydrogel electrolyte exhibit long cycle life (118 h), impressive discharge capacity (805.3 mAh g −1 ), and maximum power density (91.04 mW cm −2 ). Furthermore, 1D yarn FZABs with PVA-PAM-CMCS electrolyte are continuously produced in an integrated manner by the yarn-wrapping technique. These yarn batteries can maintain stable electrochemical performance under both bending and stretching conditions. The combined design methodology of PVA-PAM-CMCS hydrogel electrolyte and yarn-wrapping technique for 1D yarn FZABs opens a new avenue to power various portable electronics.
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