ACS Catalysis
·
2026
·
IF 13.6
Mengqi Ding,Xinhong Zhao,Yanru Li,Xuefeng Long,Hongwei Li,Dong Ji,Guixian Li
Iron-containing zeolites have shown great potential in catalyzing phenol hydroxylation with H2O2 to produce dihydroxybenzenes (catechol and hydroquinone). However, their industrial application has been hindered by low selectivity and the complexity of active-site identification. This study addresses these challenges by synthesizing a series of Fe-ZSM-5 catalysts with iron contents up to 4 wt % via an ionic liquid-assisted interzeolite transformation route. Through systematic regulation of transformation temperature and calcination atmosphere, combined with an acid treatment step, a catalytically inert benchmark sample (T180-Air) was strategically prepared and compared with three active variants (T200-Air, T180-N2, T200-N2) to achieve the isolation and identification of active sites. Employing a “differential comparison strategy”, combined with DR UV–vis, Raman, Mössbauer, XANES/EXAFS, EPR, H2-TPR, in situ FT-IR characterizations, and DFT calculations, we systematically excluded the possibility of binuclear iron species, small FexOy clusters, and distorted tetrahedral iron as primary active sites. The evidence consistently points to extra-framework octahedrally coordinated mononuclear iron as the key active species, which effectively activates both phenol and H2O2 to generate reactive intermediates. Moreover, the high-selectivity formation of dihydroxybenzenes is driven by a synergistic interaction between these mononuclear octahedral iron sites and neighboring strong acid centers. The optimal catalyst (T200-Air) achieved a phenol conversion of 46.7% and a dihydroxybenzene selectivity of 95.6% under dark conditions, with its dihydroxybenzene selectivity surpassing that of state-of-the-art Fe-based catalysts at comparable phenol conversion levels. This work elucidates the critical importance of the synergistic design between the active iron species and the local acidic microenvironment, providing clear experimental evidence and structure-performance guidelines for the rational design of high-performance iron-zeolite catalysts for selective phenol hydroxylation reactions.
✓ 已验证
产品关联
DOI
高影响因子
Autophagy
·
2026
·
IF 18.6
Didi Liu,Xiangyu Jin,Yao Zeng,Xinru Zhou,Minyu Wang,Hui Shen,Yi Huang
Insufficient lymphocyte infiltration in the tumor microenvironment is a major cause of resistance to immunotherapy. Previous studies have indicated that NLRP3 inflammasome activation is crucial for lymphocyte infiltration and activation, but safe, effective, and specific NLRP3 inflammasome agonists are still lacking. Here, we identified a clinical phase II drug, bafetinib, that specifically activates the NLRP3 inflammasome through high-throughput drug screening. Bafetinib directly targets and binds to the potassium channel KCNK6/TWIK2, blocking its degradation via the chaperone-mediated autophagy (CMA) pathway to promote potassium efflux, thereby triggering NLRP3 inflammasome activation. Furthermore, we developed a hypoxia-responsive nanoparticle platform capable of targeted delivery of bafetinib (Baf@NPs) to tumors and specific release in the tumor microenvironment. Baf@NPs treatment significantly represses tumor growth and enhances anti-tumor immunity by triggering NLRP3 inflammasome in macrophage. Moreover, the combination of Baf@NPs with PDCD1/PD-1 antibodies further enhanced anti-tumor immunity and improved tumor treatment. Together, our results identify a safe, highly effective and specific NLRP3 inflammasome agonist and underscore it enhances anti-tumor immunity by triggering the NLRP3 inflammasome in macrophages, providing a potential therapeutic strategy for tumor treatment.
✓ 已验证
产品关联
DOI
PubMed
高影响因子
JOURNAL OF FOOD SCIENCE
·
2026
·Vol. 91
·期 4
·e71020
·
IF 4.1
Kexin Wang,Yurui Zhang,Wenzheng Shi,Donglei Luan
Natural astaxanthin is a high-value carotenoid abundantly found in Antarctic krill, the efficient extraction and bioactivity enhancement of natural astaxanthin are of significant importance for its application in nutraceuticals and functional foods. However, this process faces dual challenges: the robust structural matrix of krill impedes solvent penetration, and astaxanthin predominantly exists in esterified forms with lower bioavailability. To address this, we developed an integrated autolysis process that utilizes the krill's own endogenous enzymes (proteases, lipases, and chitinases) for simultaneous matrix disruption and astaxanthin de-esterification. Compared to conventional methods that rely on exogenous enzymes or chemicals, our approach offers distinct advantages: it is environmentally benign, cost-effective, and enables simultaneous matrix disruption and astaxanthin de-esterification in a single step. Through experimental design, the optimal autolysis conditions were identified as pH 7.5, 60 ℃C, and 2 h. Under these optimized conditions, the process achieved three key outcomes: (1) An astaxanthin yield of 91.18 µg/g was achieved, representing a significant increase compared to conventional extraction methods; (2) A protein hydrolysate was simultaneously produced, with its proportion of essential amino acids substantially elevated from 12.276 ± 0.013 to 34.368 ± 0.042 mg/100 g, thereby significantly enhancing its nutritional value; (3) Effective de-esterification raised the proportion of free astaxanthin from 18.11% to 30.50%. The resulting extract demonstrated superior antioxidant activity, which was positively correlated with its free astaxanthin content. This green and cost-effective strategy represents a promising approach for the comprehensive, high-value utilization of Antarctic krill biomass in nutraceutical and functional food applications.
✓ 已验证
产品关联
DOI
高影响因子
Micromachines
·
2026
·Vol. 17
·期 4
·408
·
IF 3.5
Yaqiang Ji,Junfeng Huang,Weibin Yin,Junrui Xiang,Yongquan Liu,Yongjun Huang,Jingsheng Hong,Long Li
Bifunctional materials present a promising route to develop advanced devices, yet the dual performance of CoNi2S4nanosheets anchored on a porous scaffold is seldom reported. Herein, we propose a rational fabrication strategy to construct a three-dimensional hierarchical electrode via the in-situ growth of densely aligned CoNi2S4nanosheets on a conductive fabric scaffold. This integrated porous architecture concurrently offers an ultrahigh specific surface area, efficient mass transport, and rapid electron conduction. As a supercapacitor, the electrode achieves a high areal capacitance of 3198 mF cm−2at 4 mA cm−2and retains 98.1% of its initial capacitance after 1000 cycles at 20 mA cm−2. As a non-enzymatic glucose sensor, it exhibits outstanding selectivity (<4.1% interference), high sensitivity (1049 μA mM−1cm−2), a wide linear range (1–8 mM), and a low detection limit (1 μM). These results highlight the significant potential of this binder-free, scaffold-supported nanosheet design for advancing integrated energy storage and biosensing systems.
✓ 已验证
产品关联
DOI
高影响因子
ADVANCED MATERIALS
·
2026
·Vol. 38
·期 20
·e19832
·
IF 29.1
Dechao Gan,Xiaoyu Xie,Fei Long,Haoran Chen,Qiqing Li,Zexuan Sui,Wang Wang,Dabing Li,Yulei Chang
The second near-infrared window (NIR-II, 1000–2000 nm) provides an ideal window for multichannel photoluminescence lifetime imaging (mPLI) because of its exceptional tissue penetration depth and minimal scattering. However, the inevitable spectral crosstalk that is caused by the broad and overlapping emissions of traditional luminescent materials (e.g., organic dyes and quantum dots) has restricted the scalability of mPLI channels. Herein, we developed a series of Ln 3+ -doped (Ln = Nd, Er, Tm, or Ho) stratified nanoparticles (Ln-SNPs) that enable highly efficient multiwavelength NIR-II luminescence. These nanoparticles can be further engineered into concentration-mediated Yb 3+ energy relay nanoparticles to enable the precise tuning of the luminescence lifetime across the 1060–2050 nm range, thereby expanding the NIR-II imaging channels in mPLI. Notably, the engineered Tm-SNPs exhibit optimized downshifting emission at ∼1850 nm (NIR-IIc subwindow) with distinguishable luminescence lifetimes that span three orders of magnitude at similar emission intensities. This advancement establishes a powerful toolkit for multidimensional information encryption and multiplexed bioimaging applications.
✓ 已验证
产品关联
DOI
高影响因子
Advanced Healthcare Materials
·
2026
·
IF 11
Hong Jiang,Qin-Xie Xie,Tianzhao Tian,Qingqing Yang,Peipei Qian,Jia-Yi Liu,Wending Yan,Shengfeng Huang,Cui Shao,Wei Yi,Yifa Chen,Xu-Jia Hong
The complex tumor microenvironment (TME), characterized by coexisting normoxic and hypoxic regions, demands oxygen-adaptive nanomedicines for multimodal therapy. Herein, GTPZ is presented, an oxygen-adaptive covalent organic framework (COF)-based nanoplatform that can achieve quadruple-modal synergistic therapy through the integration of chemodynamic therapy (CDT), photothermal therapy (PTT), Type I/II photodynamic therapy (PDT), and hypoxia-activated chemotherapy. Remarkably, GTPZ exhibits a record-high photothermal conversion efficiency of 72% among COF-based nanomedicines, enabling spatiotemporal control over hypoxia-activated tirapazamine (TPZ) release to generate cytotoxic radicals. Besides, GTPZ dynamically adapts to oxygen gradients by activating Type II PDT in normoxia and switching to Type I PDT in hypoxia, with phototherapy-induced oxygen depletion amplifying TPZ activation, exhibiting high oxygen-adaptive ability. In MCF-7 models, GTPZ achieves high tumor suppression through synergistic lipid peroxidation, cell cycle arrest, and ferroptosis. This work provides a paradigm for designing intelligent nanomedicines capable of dynamically adapting to TME.
✓ 已验证
产品关联
DOI
高影响因子
MICROCHEMICAL JOURNAL
·
2026
·
IF 5.2
Biyao Zhao,Xiaoyu Wu,Xuwen Ye,Fangyi Jia,Yuanyuan Wang
The determination of acetylcholinesterase (AChE) activity and organophosphorus pesticides plays a critical role in disease diagnosis, drug screening, and food safety monitoring. In this study, we report a multifunctional UiO-66@MnO 2 nanocomposite that integrates both oxidase-mimicking activity and intrinsic fluorescence, enabling dual-mode colorimetric and fluorescence sensing. MnO 2 nanosheets anchored on the surface of the UiO-66 not only catalyze the oxidation of 3,3′,5,5′-tetramethylbenzidine (TMB) but also efficiently quenched the fluorescence of UiO-66 via Förster resonance energy transfer. Upon the addition of acetylthiocholine and AChE, the enzymatic generated thiocholine reduces MnO 2 , resulting in disassembly of the nanocomposite. This triggers a “turn-off” colorimetric signal and a “turn-on” fluorescence response, enabling sensitive detection of AChE with detection limits as low as 0.076 mU/mL (colorimetric) and 3.8 mU/mL (fluorescence). Furthermore, taking advantage of the inhibitory effect of organophosphorus pesticides on AChE activity, the proposed platform was further successfully extended to detect dipterex, achieving detection limits of 0.032 μM (colorimetric) and 1.1 μM (fluorescence). Moreover, the dual-mode nanozyme sensor was successfully validated in complex biochemical samples, highlighting its considerable potential for applications in bioanalysis, food safety monitoring, and environmental surveillance.
✓ 已验证
产品关联
DOI
高影响因子
JOURNAL OF PHARMACY AND PHARMACOLOGY
·
2026
·Vol. 78
·期 3
·
IF 3.7
Cheng Yawei,Li Gengliang,Li Yang,Ma Yugang,Xia Yujie,Zhang Chunye,Wang Xingchen
ObjectivesConventional pharmacotherapies afford only modest renoprotection in diabetic nephropathy (DN). Oleanolic acid (OA), a pentacyclic triterpenoid abundant in Traditional Chinese Medicine (TCM), mitigates DN, but its mechanism remains unclear. This study aimed to verify the hypothesis that OA protects podocytes by modulating autophagy, thereby exploring the potential therapeutic mechanism of OA in attenuating DN.MethodsHigh glucose-injured MPC5 podocytes were treated with OA to evaluate its impact on autophagy. Podocytes were further treated with graded concentrations of OA, rapamycin (an autophagy inducer), or 3-methyladenine (an autophagy inhibitor). Cell viability was quantified using the Cell Counting Kit-8 assay. Nuclear morphology was visualized by DAPI staining, autophagosomes were enumerated by transmission electron microscopy, and the expression of autophagy-related genes was determined by quantitative reverse transcription polymerase chain reaction. These complementary approaches were used to assess high-glucose-induced podocyte injury and the capacity of OA to enhance autophagy and attenuate cellular damage.Key findingsThe effective concentration window for OA was established at 5–10 μM. Within this concentration range, podocyte viability was significantly increased, accompanied by a higher autophagosome count and elevated expression of autophagosomal markers.ConclusionsOleanolic acid alleviates high glucose-induced podocyte injury by robustly activating autophagy, underscoring its potential as a sustainable therapeutic strategy for DN, along with other active compounds derived from TCM.
✓ 已验证
产品关联
DOI
高影响因子
CHEMISTRY-A EUROPEAN JOURNAL
·
2026
·
IF 3.6
Jiyong Wu,Jing Nie,Huina Wu,Dongbo Wang,Yamei Li,Minyan Liang,Huimei Qian,Yong Wang
Platinum-based chemotherapeutics remain clinically indispensable for treating various malignancies despite causing severe side effects, with ototoxicity being particularly limiting. This study reports the synthesis and evaluation of platinum(IV) prodrugs incorporating the hearing-protective ligand RG108. Among the synthesized mono- and di-substituted cisplatin and oxaliplatin derivatives, compound 4 exhibited exceptional antitumor activity with an IC 50 value of 0.07 ± 0.08 µM in FaDu cells. Mechanistic investigations revealed that the enhanced anti-tumor effect was primarily mediated via the EZH2/SLC47A2 regulatory axis. These prodrugs significantly mitigated ototoxicity, preserving cochlear hair cell viability, stabilizing auditory brainstem response thresholds, and maintaining cochlear basement membrane morphology. Our findings establish a framework for designing dual-functional platinum agents that synergize antitumor efficacy with organoprotective properties, addressing critical hearing loss limitations of conventional platinum chemotherapy while maintaining robust therapeutic outcomes. Graphical
✓ 已验证
产品关联
DOI
高影响因子
Microbiology Spectrum
·
2026
·
IF 4.1
Yuwei Qiu,Xu Dong,Dan Cao,Xiuzhi Jiang,Zhongkang Ji,Pusheng Xu,Yi Li,Kaijing Xu,Ying Zhang
Mycobacterium avium-intracellulare complex (MAC) infections are the most common nontuberculous mycobacterial infections, and ethambutol (EMB) is one of the main therapeutic agents used to treat MAC infections. However, the EMB resistance profile and its resistance mechanisms in M. avium remain poorly understood. This study determined the minimum inhibitory concentration (MIC) of 40 M. avium clinical strains, revealing that 97.5% (39/40) of the strains were intermediate (MIC = 4 µg/mL) or resistant (MIC ≥ 8 µg/mL) to EMB. One susceptible clinical isolate strain 245 (MIC = 2 µg/mL) was picked for EMB-resistant mutant isolation, and a total of 121 resistant mutants were isolated and subjected to whole-genome sequencing or Sanger sequencing. Integrated analysis revealed that 94.21% (114/121) of the mutants carried mutations in the ubiA gene, which encodes decaprenylphosphoryl-β-D-5-phosphoribose (DPPR) synthase—an enzyme involved in cell wall biosynthesis that has been associated with high-level EMB resistance in Mycobacterium tuberculosis. Complementation with the wild-type ubiA gene restored EMB susceptibility in EMB-resistant mutants and clinical strain 322 (EMB MIC = 64 µg/mL) with ubiA mutation, reducing the MICs from 32 to 4 μg/mL and from 64 to 8 μg/mL, respectively. This study indicates that the ubiA mutation is the major mechanism of EMB resistance in M. avium, which should facilitate the development of molecular tests for rapid detection of resistance in this organism.
✓ 已验证
产品关联
DOI
PubMed
高影响因子
PHYTOTHERAPY RESEARCH
·
2026
·
IF 8.1
Huimao Liu,Dan Yang,Hanyan Cheng,Lijuan Cao,Xu Song,Xun Zhou,Yuanfeng Zou,Lixia Li,Renyong Jia,Dongmei Zhang,Mingyue Li,Zhongqiong Yin
Resveratrol (RSV), a dietary polyphenol widely present in traditional medicinal plants and foods, exhibits antioxidant and anti-inflammatory properties that are relevant to ethnopharmacological strategies for protecting against environmental neurotoxicants. Given increasing real-world co-exposure to lead (Pb) and cadmium (Cd), elucidating RSV's capacity to preserve gut–brain axis (GBA) homeostasis has direct translational relevance for populations relying on phytochemical interventions. Sprague–Dawley rats were randomized into control, Pb–Cd model, and RSV treatment groups (10, 20, or 40 mg/kg). For 4 weeks, rats received Pb (300 mg/L) and Cd (50 mg/L) in drinking water with daily RSV. Cognitive function was assessed by Morris water maze; barrier integrity by Evans blue assay, histology, and Western blot for ZO-1/Occludin; synaptic ultrastructure by TEM; microbiota composition by 16S rRNA sequencing; and short-chain fatty acids (SCFAs) by GC-MS. Neurotransmitters (5-HT, GABA, SP, VIP) and cytokines (IL-6, IL-1β, TNF-α) were measured by ELISA. RSV improved spatial learning, reduced EB extravasation, preserved synaptic ultrastructure and proteins (BDNF, SYN, PSD-95), and restored intestinal architecture with increased ZO-1/Occludin. RSV attenuated cytokine release, normalized goblet cells, reversed dysbiosis by restoring Lactobacillaceae/Prevotellaceae, and increased acetate, propionate, and butyrate. It reinstated 5-HT and GABA while reducing SP and restoring VIP across serum, colon, and hippocampus. RSV attenuated Pb–Cd–associated neurotoxicity and was accompanied by improved intestinal and BBB-related readouts, partial normalization of gut microbiota features and SCFA levels, and preservation of synaptic and neurotransmitter-related markers, consistent with a link to gut–brain axis function. This study is among the first to test RSV in a Pb–Cd co-exposure model using a multi-dose regimen with integrated behavioral, barrier, microbial, and neurochemical endpoints.
✓ 已验证
产品关联
DOI
PubMed
高影响因子
JOURNAL OF NANOBIOTECHNOLOGY
·
2026
·
IF 15
Cai Ming,Liu Zhao,Sun Xun,Liao Ziming,Gou Yu,Yang Xuan,Qi Ying,Xing Guosheng,Liu Tingting,Zhao Wenjun,Duan Xiaoyuan,Zhang Tao,Yao Bin,Xu Weiguo
Chronic diabetic wounds are often complicated by persistent infections and oxidative stress, which impede effective healing. In this study, we developed an injectable, dual-responsive nanocomposite hydrogel designed to simultaneously scavenge reactive oxygen species (ROS) and deliver photothermal and photodynamic therapy for wound treatment. The hydrogel was synthesized via dynamic boronate-ester crosslinking of poly(vinyl alcohol) (PVA) with a bis-boronic acid linker (TSPBA), enabling efficient ROS quenching. Incorporation of palygorskite/molybdenum disulfide (PGS/MoS₂) nanocomposites imparted photothermal and photodynamic properties, activated by NIR light. In vitro tests demonstrated that the PVA-TSPBA@PGS/MoS₂ hydrogel effectively scavenged ROS, induced mild hyperthermia (~ 48 °C) under NIR, and exhibited significant antibacterial activity against Staphylococcus aureus. In vivo, the hydrogel accelerated wound closure in a diabetic mouse model, with a 46.7% residual area at day 7, compared to higher residual areas in control groups (66.5–92.4%). By day 14, nearly complete wound healing was achieved, accompanied by reduced bacterial load and inflammatory markers, as well as enhanced re-epithelialization, angiogenesis, and collagen remodeling. These findings suggest that this multifunctional hydrogel represents a promising strategy for the treatment of infected diabetic wounds, offering a controlled, localized approach to wound healing. Graphical
✓ 已验证
产品关联
DOI
PubMed
高影响因子
危险品化学品经营许可证（带存储）