Reaction Kinetics Mechanisms and Catalysis
·
2021
·Vol. 134
·期 1
·243-257
·
IF 2.081
Zhang Wenfei,Zhou Qulan,Qi Ji,Li Na
Cu 2 O films were prepared using an improved solution immersion method, whereas ZnO/Cu 2 O films were prepared using an electrodeposition method. Then, the pure Cu 2 O film electrodes and ZnO/Cu 2 O film electrodes were used to reduce CO 2 to CH 3 OH. The crystal structure, morphology, particle size, and specific surface area of pure Cu 2 O films prepared under different immersion times and ZnO/Cu 2 O films prepared under different deposition times and Zn 2+ concentrations were analyzed using X-ray diffraction, scanning electron microscopy, and Brunauer–Emmett–Teller analysis. The electrolytic activity of the pure Cu 2 O film electrodes and ZnO/Cu 2 O film electrodes were studied. Under the same reaction conditions, the ZnO/Cu 2 O film electrode with the Zn 2+ concentration of 0.05 M and the deposition time of 30 min had the highest total CH 3 OH yield of 315.656 μmol/cm 2 and the fastest formation rate of 52.609 μmol/(cm 2 h), and its faradaic efficiency was 45%, which was remarkably higher than that of pure Cu 2 O film electrodes under the same reaction conditions. This study highlighted that ZnO nanoparticles had a very good promotion of electrolytic CO 2 reduction on Cu 2 O film.
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高影响因子
JOURNAL OF NANOBIOTECHNOLOGY
·
2021
·Vol. 19
·期 1
·1-20
·
IF 10.435
Chen Yan,Fang Lei,Zhou Weixin,Chang Jinghan,Zhang Xiaojuan,He Chuanchuan,Chen Chen,Yan Ruicong,Yan Yakai,Lu Yao,Xu Chuanrui,Xiang Guangya
Background Hypoxic tumor microenvironment (TME) promotes tumor metastasis and drug resistance, leading to low efficiency of cancer chemotherapy. The development of targeted agents or multi-target therapies regulating hypoxic microenvironment is an important approach to overcome drug resistance and metastasis. Methods In this study, chitosan oligosaccharide (COS)-coated and sialic acid (SA) receptor-targeted nano-micelles were prepared using film dispersion method to co-deliver cisplatin (CDDP) and nitric oxide (NO) (denoted as CTP/CDDP). In addition, we explored the mechanisms by which NO reversed CDDP resistance as well as enhanced anti-metastatic efficacy in hypoxic cancer cells. Results Because of the different affinities of COS and SA to phenylboronic acid (PBA) under different pH regimes, CTP/CDDP micelles with intelligent targeting property increased cellular uptake of CDDP and enhanced cytotoxicity to tumors, but reduced systemic toxicity to normal organs or tissues. In addition, CTP/CDDP showed stimulus-responsive release in TME. In terms of anti-tumor mechanism, CTP/CDDP reduced CDDP efflux and inhibited epithelial-mesenchymal transition (EMT) process of tumor by down-regulating hypoxia-inducible factor-1α (HIF-1α), glutathione (GSH), multidrug resistance-associated protein 2 (MRP2) and matrix metalloproteinase 9 (MMP9) expression, thus reversing drug resistance and metastasis of hypoxic tumor cells. Conclusions The designed micelles significantly enhanced anti-tumor effects both in vitro and in vivo. These results suggested that CTP/CDDP represented a promising strategy to treat resistance and metastatic tumors. Graphic abstract
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PubMed
高影响因子
RARE METALS
·
2021
·Vol. 41
·期 2
·700-712
·
IF 4.003
Gu Ya-Hui,Liu Hong-Wei,Dong Xiao-Han,Ma Zhuang-Zhuang,Li You-Xin,Li Li,Gan Dong-Lin,Liu Ping-Sheng,Shen Jian
Antifouling ability and blood compatibility are critically important in the development of medical metallic implants for clinical applications. Here, we report the zwitterionic-phosphonate block polymer as a new type of high-efficiency antifouling coating for metallic substrates. Six block polymers (pSBMA- b -pDEMMP) with different segment lengths ( n SBMA : n DEMMP = 10:25, 40:25, 100:25, 75:5, 75:40, 75:100) were prepared and anchored on titanium alloy (TC4) substrates. 1 H nuclear magnetic resonance (NMR) results clearly showed the precise preparation of the block polymers. XPS analysis and water contact angle measurement indicated the successful construction of the block polymer on TC4 substrates. The relationship between the antifouling performance of the polymer coating and the length of pDEMMP and pSBMA segments in the block polymer was established. Results showed that the polymer containing the pSBMA segment above 40 repeat units could significantly inhibit protein adsorption, platelet adhesion, bacterial adhesion and cell adhesion, while the pDEMMP segment above 5 repeat units is able to generate stable zwitterionic polymer coating on TC4 substrates. This ease of production and high-efficiency antifouling modification strategy elucidated here may find broad application for biomedical implants and devices in clinical applications. Graphic abstract
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高影响因子
EUROPEAN FOOD RESEARCH AND TECHNOLOGY
·
2021
·Vol. 247
·期 9
·2171-2181
·
IF 2.998
Zhang Bo,Li Hongzhou,Li Yan,Fu Xiaoling,Du Daolin
Sensitive and rapid analysis of zearalenone (ZEN) in grains and feed is essential to guarantee the food security for human health. Herein a chemiluminescence method was demonstrated to measure the content of zearalenone in food samples. An immunomagnetic beads-anti-ZEN monoclonal antibody complex was prepared. Besides, the immunomagnetic beads were utilized as the solid support for rendering the immunoassay through the rapid formation of immune complexes in the solution, rapid separation and efficient heterogeneous detection. Under the optimal experimental parameters, the standard curve of ZEN detection is y = − 0.3839x + 0.1565 ( R 2 = 0.9978), the signal ratio has a well linear relationship with the logarithm of ZEN concentration in the range of 0.03–2.43 ng/mL. The limit detection of zearalenone in the developed assay was 0.01 ng/mL. The applicability of the reported approach for zearalenone detection in grains was demonstrated. In addition, the good recoveries of zearalenone were obtained, ranging from 85.15 to 102.60% at 5, 50, 150 ng/g spiked ZEN concentrations. The relative standard deviation was < 10%. The proposed chemiluminescence immunoassay is sensitive, simple, rapid and reliable for monitoring of zearalenone contamination, which provides a powerful avenue for further application of other mycotoxin in food analysis.
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Microbial Cell Factories
·
2021
·Vol. 20
·期 1
·1-12
·
IF 5.328
Jia Hexue,Sun Wan,Li Xuezhi,Zhao Jian
Background Trichoderma reesei is currently the main strain for the commercial production of cellulase. Cellulose induced protein 1 (Cip1) is one of the most abundant proteins in extracellular proteins of T. reesei. Reported literatures about Cip1 mainly focused on the regulation of Cip1 and its possible enzyme activities, but the effect of Cip1 on the enzymatic hydrolysis of lignocellulose and possible mechanism have not still been reported.Results In this study, Cip1 from T. reesei was cloned, expressed and purified, and its effects on enzymatic hydrolysis of several different pretreated lignocellulose were investigated. It was found that Cip1 could promote the enzymatic hydrolysis of pretreated lignocellulose, and the promoting effect was significantly better than that of bovine serum albumin (BSA). And especially for the lignocellulosic substrate with high lignin content such as liquid hot water pretreated corn stover and corncob residue, the promoting effect of Cip1 was even better than that of the commercial cellulase when adding equal amount protein. It was also showed that the metal ions Zn2+ and Cu2+ influenced the promoting effect on enzymatic hydrolysis. The Cip1 protein had no lyase activity, but it could destroy the crystal structure of cellulose and reduce the non-productive adsorption of cellulase on lignin, which partly interpreted the promoting effect of Cip1 on enzymatic hydrolysis of lignocellulose.Conclusion The Cip1 from T. reesei could significantly promote the enzymatic hydrolysis of pretreated lignocellulose, and the promotion of Cip1 was even higher than that of commercial cellulase in the enzymatic hydrolysis of the substrates with high lignin content. This study will help us to better optimize cellulase to improve its ability to degrade lignocellulose, thereby reducing the cost of enzymes required for enzymatic hydrolysis.
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PubMed
高影响因子
IONICS
·
2021
·Vol. 27
·期 11
·4849-4857
·
IF 2.817
Hussain Saddam,Li Yangping,Mustehsin Ali,Ali Akbar,Thebo Khalid Hussain,Ali Zeeshan,Hussain Sabir
In this work, we prepare samarium-doped ceria (SDC) nanocomposite electrolytes by the co-precipitation method with different ZnO:SDC weight ratios for solid oxide fuel cell application. The physicochemical characterization of the nanocomposite electrolytes is carried out by X-ray diffraction (XRD), scanning electron microscopy (SEM), X-ray photoelectron spectroscopy (XPS), thermogravimetric analysis (TGA), and differential scanning calorimetry (DSC) techniques. Electrochemical impedance spectroscopy is performed to measure the electrical properties of the electrolytes, and the results show that the optimum composition of ZnO ( x = 0.2) nanocomposite demonstrates a better ionic conductivity ~ 0.1–0.7 S/cm at 350–700 °C. The fuel cell performance of the ZnO/SDC nanocomposite electrolytes is measured with hydrogen as the fuel. The as-synthesized nanocomposite material with ZnO ( x = 0.2) also exhibits a power density of 804 mW/cm 2 along with a maximum open-circuit voltage (OCV) of 1.10 V at 700 °C.
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高影响因子
ANALYTICAL AND BIOANALYTICAL CHEMISTRY
·
2021
·Vol. 413
·期 25
·6225-6237
·
IF 4.157
Dong Wei-Chong,Guo Jia-Liang,Zhao Meng-Qiang,Wu Xi-Kun,Cui Yi-Xuan,Feng Jing-Ying,Zhang Chen-Xiao,Jiang Ye,Zhang Zhi-Qing
The presence of reduced aminothiols, including homocysteine (Hcy), cysteine (Cys), cysteinyl-glycine (CG), and glutathione (GSH), is significantly increased in the pathological state. However, there have been no reports on the relationship between reduced aminothiols (Hcy, Cys, CG, and GSH) and different genders, ages, and drug combinations in human blood. The accurate quantification of these reduced thiols in biological fluids is important for monitoring some special pathological conditions of humans. However, the published methods typically not only require cumbersome and technically challenging processing procedures to ensure reliable measurements, but are also laborious and time-consuming, which may disturb the initial physiological balance and lead to inaccurate results. We developed a hollow fiber centrifugal ultrafiltration (HFCF-UF) method for sample preparation coupled with a high-performance liquid chromatography–tandem mass spectrometry (HPLC-MS/MS) method and used it to determine four reduced aminothiols (Hcy, Cys, CG, and GSH) in human blood for the first time. A total of 96 clinical patients were enrolled in our study. The influence of different genders, ages, and drug combinations on the levels of four reduced thiols in human blood was also discussed by SPSS 24.0. The sample preparation was simplified to a single 5 min centrifugation step in a sealed system that did not disturb the physiological environment. The validation parameters for the methodological results were excellent. The procedure was successfully applied to monitoring the concentrations of four reduced aminothiols (Hcy, Cys, CG, and GSH) in 96 clinical blood samples. There were no significant differences in Hcy, Cys, CG, or GSH for the different genders, ages, or combinations with methotrexate or vancomycin ( P > 0.05). However, there was a significant increase in Hcy concentration in patients treated with valproic acid who were diagnosed with epilepsy ( p =0.0007). It is advisable to measure reduced Hcy level in patients taking valproic acid. The developed HFCF-UF method was simple and accurate. It can be easily applied in clinical research to evaluate oxidative stress in further study. Graphical abstract
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高影响因子
JOURNAL OF WUHAN UNIVERSITY OF TECHNOLOGY-MATERIALS SCIENCE EDITION
·
2021
·Vol. 36
·期 2
·183-188
·
IF 0.957
Zuo Jiaxiang,Jin Xin,Zeng Hui
Fresh EPF proteins from Liangzi Lake were employed as a template to synthesize hierarchically porous nitrogen-doped TiO 2 in a single process. The experimental results show that N-TiO 2 sample exhibits significantly enhanced visible-light photocatalytic activity in both chemical waste treatment and hydrogen production, and the visible-light photocatalytic activities vary with the concentrations of EPF proteins. The optimal concentration of protein is 600 mg·mL −1 and the degradation of RhB could be almost completed in just 25 min. Furthermore, the performance of as-synthesized TiO 2 as an electrode for Li-ion battery can be also regulated by the EPF proteins. Natural extrapallial fluid (EPF) proteins extracted from the same kind of mussels living in different regions have significantly different effects on the performances of TiO 2 as electrode materials for Li-ion battery. The present work highlights the unimaginable effects of natural organic matrix on the synthesis of advanced materials with optimized functional properties.
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高影响因子
BIODEGRADATION
·
2021
·Vol. 32
·期 6
·677-695
·
IF 3.909
Tong Qibang,Wang Guixin,Chen Maolian,Chen Yaping,Guo Yong
Biofilm process is a promising wastewater treatment technology and biofilm carrier (biocarrier) is regarded as the core of this process. However, the traditional commercial biocarriers have their inherent drawbacks, therefore, the development of new-type biocarrier to enhance wastewater treatment efficiency is significantly important to biofilm-based reactors. In this study, based on radical suspension polymerization, a novel kind of magnetic porous carriers (PMCs) was prepared by modifying the porous polymer carriers (PPCs) with inorganic particles, and then applied in a fluidized bed bioreactor (FBBR) with a low packing ratio of 10 % (v/v) to synthetic wastewater treatment. The results showed that this novel biocarrier possesses paramagnetism with saturation magnetization of 1.01emu/g, low density (1.26 g/cm 3 ), excellent hydrophilicity (surface water contact angle approaching zero) and rough surface. Besides, compared with the PPCs, the developed PMCs have larger pores (up to 50 μm or more), in which the larger-sized microbes are able to colonize. Moreover, as compared to the PPCs-based FBBR, the PMCs-based reactor achieved shorter time (7 days) for biofilm formaiton and significantly enhanced NH 3 -N removal efficiency ( nearly 20 % increase at the level of influent NH 3 -N concentration about 100 mg/L). High-throughput sequencing (HTS) results indicated that this new biocarrier could promote biodiversity and improve the abundance of Nitrosomonadales (the functional bacteria for ammonia removal in the bio-system), thus enhancing the ammonification process. Therefore, the developed PMCs could be preferable biocarriers for biofilm formation and provide an alternative to the traditional suspended biocarrier, demonstrating a promising potential, even at a lower filling ratio, to enhance the pollutants removal performance.
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高影响因子
PLANT AND SOIL
·
2021
·Vol. 468
·期 1
·491-507
·
IF 4.192
Zhang Yijie,Li Tianyao,Ye Chen,Lu Renchuang,Liu Yixiang,Huang Huichuan,He Xiahong,Yang Min,Zhu Shusheng
Background and aims Overaccumulation of allelochemicals in soil from trees has a negative effect on the growth of Panax notoginseng and cause root rot. Many plant secreted phenols are water-soluble substances that can be depleted by leaching. However, comprehensive understanding of the effect of leaching of phenols in Chinese fir soil on P. notoginseng root rot disease is still lacking. Methods Soil with high level of phenols was leached with five intensities and their effect on the root rot of P. notoginseng was assessed. Then, the relationship of root rot with changes in soil chemical properties and phenol content as well as rhizospheric microorganisms was analyzed. Results With increasing leaching intensity, the levels of phenols in soils and the incidence of root rot decreased significantly. Decreased phenol content could alleviate root rot by modifying the soil microbial community. Network analysis showed that root rot incidence was positively correlated with the p-hydroxybenzoic acid-enriched pathogenic fungus Ilyonectria but negatively correlated with p-hydroxybenzoic acid (HA)-, ferulic acid (FA)- or vanillic acid (VA)-suppressed bacteria, including Sphingomonas , Lysobacter , Massilia and Burkholderia . Culture experiments confirmed that individual HA, FA and VA as well as a mixture of the five phenols with increasing concentrations could stimulate the growth and sporulation of I. destructans but suppress the growth and biofilm formation of bacteria ( Burkholderia and Lysobacter ). Moreover, Burkholderia isolates showed antagonistic activity against the growth of I. destructans . Conclusions Leaching could relieve the stimulation of pathogenic fungi by phenols and reduce the pressure on phenolic-suppressed bacteria, thereby reducing the incidence of root rot disease.
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高影响因子
RARE METALS
·
2021
·Vol. 41
·期 2
·650-662
·
IF 4.003
Guo Hua,Li Jun,Zou Xian-Rui,Wang Hong-Shui,Kang An,Zhou Huan,Li Ming-Jun,Zhao Xiao-Yan
Abstract Development of photocatalytic materials able to profit from the whole spectrum of sun for efficient organic pollution removal and bacterial elimination is a fascinating strategy in environmental engineering. In current work, GO-TiO 2 /(Ca,Y)F 2 :Tm,Yb, a full-spectrum composite photocatalyst, was prepared via hydrothermal processing with TiO 2 /(Ca,Y)F 2 :Tm,Yb particles deposited on the surface of graphene oxide (GO). The surface properties of GO-TiO 2 /(Ca,Y)F 2 :Tm,Yb were characterized using various characterization techniques including X-ray diffraction (XRD), scanning electron microscopy (SEM), X-ray photoelectron spectroscopy (XPS) and Brunauer–Emmett–Teller (BET) surface area analyzer. Besides, ultraviolet–visible (UV–Vis) diffuse reflectance spectroscopy, electron spin resonance (ESR) and other methods were used to systematically explore the photocatalytic mechanism of GO enhancement of TiO 2 /(Ca,Y)F 2 :Tm,Yb. In photocatalytic degradation study, the degradation rate of methyl orange (MO) was improved by 40% when GO was added into the TiO 2 /(Ca,Y)F 2 :Tm,Yb system. In addition, the presence of GO also improves the antibacterial ability of TiO 2 /(Ca,Y)F 2 :Tm,Yb against Escherichia coli and Staphylococcus aureus . All results reveal high efficacy of GO-TiO 2 /(Ca,Y)F 2 :Tm,Yb under full spectrum light irradiation, in which (Ca,Y)F 2 :Tm,Yb and TiO 2 can improve its utilization of full spectrum sunlight, and GO can enhance adsorption ability of dye and electron–hole pair differentiation ability of TiO 2 /(Ca,Y)F 2 :Tm,Yb. Graphic abstract
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高影响因子
CELLULOSE
·
2021
·Vol. 28
·期 16
·10373-10384
·
IF 5.044
Cao Qinghua,Dai Jinyue,Bao Xin,Zhang Zhenyu,Liu Fei,Feng Yuhong,Na Haining,Zhu Jin
Abstract A series of cellulose-based fluorescent materials are prepared under relative mild conditions by use of the DMSO/1,8-diazabicyclo[5.4.0]undec-7-ene (DBU)/CO 2 system to utilize as coating pigments. Through the observation under 365 nm UV light, the cellulose-based fluorescent materials exhibit good fluorescence response and bright color. Furthermore, due to the limitation of the molecular skeleton of cellulose, the intrinsic aggregation caused quenching phenomenon commonly existed in conventional organic fluorescent pigments can be effectively inhibited, which is very helpful to retain good fluorescence response in epoxy-based coating material and its coating films. Moreover, the addition of cellulose-based fluorescent materials also increases the mechanical properties of the coating film. The increase of tensile strength and tensile modulus respectively reaches ~39% and ~66%. Solvent resistance and thermal property of the coating films generally remain unchanged. The fabrication of cellulose-based fluorescent materials in DMSO/DBU/CO 2 system provides a feasible way to develop the functional application of cellulose. Graphic abstract
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高影响因子
危险品化学品经营许可证（带存储）