MACROMOLECULAR BIOSCIENCE
·
2024
·
IF 4.4
Siyuan Yu,Xian Shao,Tong Wu,Zheng Liu,Pei Yu,Jinfeng Xing
In order to achieve long-term and controllable release of anti-tumor drugs at specific sites, temperature/pH responsive nanoparticles encapsulating 5-fluorouracil and methotrexate in situ are prepared through dispersion photopolymerization under green LED irradiation. The physicochemical properties of nanoparticles are characterized by scanning electron microscopy, Fourier transform infrared, dynamic light scattering, thermogravimetric/differential scanning calorimetry, and X-ray diffraction. In vitro drug release at different temperatures and pH values is examined to ascertain the release pattern of two drugs, which can be well described by Korsmeyer–Peppas kinetic model. The cytotoxicity evaluation illustrates that the tumor cells could be more effectively killed by the drug-loaded nanoparticles, and the improved therapeutic effect is attributed to the controllable and sustainable drug release as well as the enhanced cellular uptake. The blood safety and good biocompatibility of nanoparticles are further confirmed by hemolysis assay, indicating the prepared nanoparticles are potential candidates for effective tumor treatment.
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产品关联
DOI
PubMed
高影响因子
Synthetic and Systems Biotechnology
·
2025
·
IF 4.4
Yanghao Liu,Changgeng Li,Yu Miao,Zhichao Chen,Tangen Shi,Ling Ma,Siyu Zhao,Zichen Yu,Lanxiao Li,Qingyang Xu
5-Aminolevulinic acid (5-ALA) is an important non-proteinogenic amino acid with wide applications in agriculture and medicine. To achieve high-level microbial production, this study established a dual-pathway reconstruction strategy in Escherichia coli by integrating the endogenous C5 pathway with an inducible exogenous C4 pathway. Multi-copy overexpression of gltX , hemA , and hemL , combined with enhanced glutamate supply and the introduction of a non-oxidative glycolysis pathway, effectively increased the C5 pathway flux and carbon efficiency, while product toxicity was significantly alleviated by strengthening efflux mechanisms and oxidative stress tolerance. A quorum sensing–based regulatory system was employed to dynamically regulate hemB expression, thereby balancing cell growth and 5-ALA biosynthesis, and a controlled glycine feeding strategy specifically activated the C4 pathway during the later fermentation stage, forming a stage-specific dynamic activation mechanism. Promoter regulation of sucC/sucD expression and enhanced endogenous PLP biosynthesis further stabilized the C4 flux. Fed-batch fermentation in a 5 L bioreactor resulted in a final 5-ALA titer of 37.34 g/L, demonstrating the industrial potential of this systems metabolic engineering strategy combining dual pathways and stage-specific activation control.
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产品关联
DOI
PubMed
高影响因子
Frontiers in Chemistry
·
2021
·
IF 5.221
Liu Can,Zhang Liming,Tan Li,Liu Yueping,Tian Weiqian,Ma Lanqing
In this study, we immobilized pectinase preparation on porous zeolite ZSM-5 as an enzyme carrier. We realized this immobilized enzyme catalyst, pectinase preparation@ZSM-5, via a simple combined strategy involving the van der Waals adsorption of pectinase preparation followed by crosslinking of the adsorbed pectinase preparation with glutaraldehyde over ZSM-5. Conformal pectinase preparation coverage of various ZSM-5 supports was achieved for the as-prepared pectinase preparation@ZSM-5. The porous pectinase preparation@ZSM-5 catalyst exhibited ultra-efficient biocatalytic activity for hydrolyzing the β-glycosidic bonds in the model substrate 4-nitrophenyl β-D-glucopyranoside, with a broad operating temperature range, high thermal stability, and excellent reusability. The relative activity of pectinase preparation@ZSM-5 at a high temperature (70 °C) was nine times higher than that of free pectinase preparation. Using thermal inactivation kinetic analysis based on the Arrhenius law, pectinase preparation@ZSM-5 showed higher activation energy for denaturation (315 kJ mol−1) and a longer half-life (62 min−1) than free pectinase preparation. Moreover, a Michaelis–Menten enzyme kinetic analysis indicated a higher maximal reaction velocity for pectinase preparation@ZSM-5 (0.22 µmol mg−1 min−1). This enhanced reactivity was attributed to the microstructure of the immobilized pectinase preparation@ZSM-5, which offered a heterogeneous reaction system that decreased the substrate–pectinase preparation binding affinity and modulated the kinetic characteristics of the enzyme. Additionally, pectinase preparation@ZSM-5 showed the best ethanol tolerance among all the reported pectinase preparation-immobilized catalysts, and an activity 247% higher than that of free pectinase preparation at a 10% (v/v) ethanol concentration was measured. Furthermore, pectinase preparation@ZSM-5 exhibited potential for practical engineering applications, promoting the hydrolysis of β-glycosidic bonds in baicalin to convert it into baicalein. This was achieved with a 98% conversion rate, i.e., 320% higher than that of the free enzyme.
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DOI
PubMed
高影响因子
MATERIALS RESEARCH BULLETIN
·
2022
·
IF 5.6
Huazhen Liao,Song Ye,Jingxin Ding,Jianzhong Yu,Xinling Xv,Ling Pan,Peixuan Lin,Deping Wang
Enabling the drug delivery systems with drug release monitoring function is of essential importance to enhance therapeutic effectiveness. Herein, we took a ship-in-a-bottle synthesis strategy to construct the upconversion nanocrystal and zeolite compounded drug delivery system with real-time monitoring function, by using desilicated ZSM-5 zeolite as drug carrier and NaYF 4 : Yb 3+ /Tm 3+ nanocrystal as luminescent probe. Benefiting from the enlarged mesoporous in desilicated ZSM-5, the NaYF 4 : Yb 3+ /Tm 3+ nanocrystals can be successfully loaded inside and the upconversion emission intensity of Tm 3+ under 980 nm excitation was one order of magnitude enhanced after mild heat treatment. The drug release monitoring function were studied by using doxorubicin as model drug, the experimental results indicated that the upconversion emission intensity ratio of I 802nm /I 475nm can accurately evaluate drug release percentage. Further in vitro cytotoxicity evaluation suggested the doxorubicin-loaded [email protected] /DS/HT has excellent biocompatibility and is able to inhibit tumor cells effectively.
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DOI
高影响因子
Chinese Medicine
·
2026
·Vol. 21
·期 1
·230
·
IF 7.4
Wang Shasha,Huang Kexuan,Cheng Yuhan,Sun Cheng,Wang Kan,He Qirui,Yin Gang,Tang Decai,Zhang Yu,Tan Xiying
Background Astragali Radix (HQ) and Curcumae Rhizoma (EZ), collectively termed AC, are commonly used as an herb pair in Traditional Chinese Medicine (TCM) for colorectal cancer (CRC). Although AC has been used as an adjuvant to chemotherapy, the molecular basis by which it enhances the efficacy of 5-fluorouracil (5-FU) remains incompletely defined. Methods The antitumor effect of AC alone and in combination with 5-FU was evaluated in a HCT116 xenograft mouse model and in CRC cell lines with different genetic backgrounds. Potential systemic toxicity was assessed using serum biochemical markers. Transcriptomic changes induced by AC were analyzed by RNA sequencing and integrated with network pharmacology and The Gene Expression Omnibus (GEO) (GSE17536, GSE17537, GSE41258, GSE20916). Key targets were validated by Western blotting and immunofluorescence. Cell viability, drug interaction, apoptosis, cell-cycle distribution and DNA damage were assessed using CCK-8 assays, Synergy Finder analysis, flow cytometry, and γ-H2AX staining. Bioavailable AC-derived constituents were characterized by UPLC-QTOF-MS, and candidate active compounds were further evaluated by molecular docking, molecular dynamics simulation, cellular thermal shift assay, and functional validation. Results AC potentiated the antitumor efficacy of 5-FU in vivo and did not exacerbate 5-FU-associated hepatic or renal biochemical toxicity under the tested conditions. In vitro, AC-containing serum extracts inhibited CRC cell viability, induced reversible G2/M-phase arrest, and enhanced 5-FU sensitivity in HCT116, CT26, and HCT15 cells. AC treatment caused limited early apoptosis, whereas delayed apoptosis was observed after prolonged exposure, suggesting that cell-cycle arrest represents the primary early response. RNA-seq and integrative analyses showed that AC suppressed pathways associated with DNA replication, mismatch repair, ribosome biogenesis, and cell-cycle progression. AC downregulated key regulators involved in cell-cycle control, DNA repair, and stress-survival signaling, including PLK1, CDK1, CCNB1, CDC25A, c-JUN, MSH2, BRCA1, and p-AKT. Combination treatment further increased γ-H2AX accumulation and reduced cell-cycle regulatory proteins. Functional validation indicated that c-JUN overexpression partially attenuated AC-mediated 5-FU sensitization, whereas pharmacological AKT inhibition partially mimicked the sensitizing effect of AC. Among the serum-exposed constituents, Demethoxycurcumin and Kumatakenin were identified as bioavailable active compounds that partially recapitulated the effects of AC by inducing G2/M-phase arrest and enhancing the antiproliferative activity of 5-FU. Molecular docking, molecular dynamics simulation, and CETSA supported their target engagement with cell-cycle regulators, particularly CCNB1 and CDK1. Conclusion AC enhances the therapeutic efficacy of 5-FU in CRC by weakening DNA repair-cell-cycle coordination and stress-survival signaling, thereby increasing 5-FU-induced DNA damage and promoting cell-cycle arrest. Demethoxycurcumin and Kumatakenin may represent important bioavailable contributors to this chemosensitizing effect, although the complete AC extract likely acts through multi-component and multi-target mechanisms. These findings provide mechanistic support for the use of AC as an adjunctive strategy to improve 5-FU-based chemotherapy in CRC. Graphical
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高影响因子
SEPARATION AND PURIFICATION TECHNOLOGY
·
2022
·
IF 9.136
Xin Yu,Xiaoqing Dang,Shijie Li,Yu Li,He Wang,Kairui Jing,Haoyun Dong,Xia Liu
Metal-support interaction has a substantial influence on catalyst activity while little attention has been paid to it in the plasma catalytic system. Herein, the effect of support on the activity of CoMnO X -based catalysts was investigated for trichloroethylene removal by plasma catalysis, and CoMnO X /ZSM-5 exhibited the highest mineralization rate (36.93−94.24 %), HCl and Cl 2 yield (19.66−94.37 %), energy yield (63.29–29 g/kWh), as well as the lowest export ozone concentration (18.72–108.83 mg/m 3 ) under 6–10 kV. The excellent catalytic activity of CoMnO X /ZSM-5 was ascribed to the formation of abundant defects and oxygen vacancies , which was induced by the Si O Co/Mn hybridization promoting Co/Mn O bonds cleavage. CoMnO X /ZSM-5 also exhibited superior adsorption ability, large amount of Co 3+ , Mn 4+ and adsorbed oxygen species, high reducibility and suitable acidity. Moreover, CoMnO X /ZSM-5 maintained superior water-resistant ability and high catalytic activity after 50 h operation. The proposed TCE degradation mechanism suggested that the contribution of plasma active oxygen species was involved in TCE oxidation and oxygen cycle of catalyst. This work provides a novel strategy to design catalysts in plasma catalytic system.
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DOI
高影响因子
CHINESE JOURNAL OF CHEMICAL ENGINEERING
·
2023
·
IF 3.8
Xiaoxue Zhang,Shuman Xu,Jing Hao,Xiaojin Xie,Fengqiu Chen,Dang guo Cheng
Single-crystalline hierarchical ZSM-5 zeolites with different particle sizes (namely 100, 140, and 200 nm) were successfully prepared by adjusting the amount of tetrapropylammonium hydroxide (TPAOH), and investigated in n -heptane catalytic cracking reaction. Diffusional measurements by zero-length column (ZLC) method showed that the apparent diffusivities of n -heptane decreased with the reduction of particle size, indicating the existence of surface barriers. Moreover, with the decrease of particle size, the additional diffusion path length increased, which meant the influence of surface barriers became more apparent. Despite the change of surface barriers, the intracrystalline diffusion still dominated the overall diffusion. Catalytic performance showed that the zeolite with smaller particle size had better stability.
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DOI
高影响因子
CRYSTAL GROWTH & DESIGN
·
2017
·
IF 4.055
Tao Liu,Ying-Hui Yu,Han-Zhong Zhang,Wen-Hong Jiang,Jin-Sheng Gao,Guang-Feng Hou
Hydrothermal reactions of rare earth (Nd, Sm, Eu, and Tb) and l- or d-lactic acid are used to construct four pairs of homochiral metal–organic frameworks (HMOFs) with polymetallic building blocks, namely, {[Nd2(L)2(H2O)3]·4H2O}n (1-r)/(1-s) and {[Ln2(L)2(H2O)2]·4H2O}n {Ln = Sm (2), Eu (3), Tb (4)} (n-r)/(n-s). The eight complexes were characterized by single-crystal X-ray diffraction, infrared spectra, elemental analysis, thermogravimetric analysis, electronic circular dichroism (ECD) spectra, and powder X-ray diffraction measurements. The complexes 1–4 featuring a 3D porous metal–organic framework crystallize in chiral space groups, and their chirality is proven by the solid-state ECD spectra. Moreover, the second-harmonic generation (SHG) investigation reveals that the complexes 1-r, 2-r, 3-r, and 4-r have medium SHG eficiencies. The complexes 2, 3, and 4 exhibit relatively high luminescence properties, which could be ascribed to ligand-centered emission. The luminescence of complex 4-r could be quenched by nitrobenzaldehyde, which may be used for molecular sensing and recognition.
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DOI
高影响因子
Scientific Reports
·
2017
·Vol. 7
·期 1
·1-9
·
IF 4.259
Wang Chao,Zhang Liming,Zhou Tian,Chen Jiachuan,Xu Feng
5-hydroxymethylfurfural (HMF) is an important platform molecule in the synthesis of various chemicals and materials. Herein, we reported a simple and effective dehydration of glucose-based carbohydrates to HMF in a biphasic system containing cyclopentyl methyl ether as the organic phase and AlCl 3 with minute amounts of HCl as co-catalysts. The results showed that the mixed catalysts had a positive synergistic catalytic effect on glucose conversion to HMF compared with single AlCl 3 or HCl catalyst. For glucose, the highest HMF yield of 54.5% was achieved at 175 °C for 20 min. More importantly, the optimal catalytic system was so efficient that it achieved one of the highest reported yields of HMF (30.5%) directly from corncob acid hydrolysis residues. Thus, the catalytic system can become a promising route for effective utilization of biomass in future biorefineries.
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DOI
PubMed
高影响因子
RSC Advances
·
2016
·Vol. 6
·期 106
·104398-104406
·
IF 3.289
Cong Zhang,Jingbo Qi,Jing Xing,Si-Fu Tang,Liang Song,Yuanyuan Sun,Chuanhui Zhang,Hongchuan Xin,Xuebing Li
Aqueous phase catalytic upgrading of lignin monomers to hydrocarbons via hydrodeoxygenation (HDO) has been explored using a combination of Pd/C and HZSM-5 catalysts under 2 MPa of H2 (ambient temperature). Model monomers with varying numbers of methoxy groups, including phenol, anisole, guaiacol and 2,6-dimethoxy-phenol, were chosen as lignin model compounds. Mechanistic studies revealed cascade and parallel reaction pathways via hydrogenation and dehydration (hydrolysis) processes, which were catalyzed by Pd/C and HZSM-5, respectively. Hydrogenation was preferred at lower temperature, whereas higher temperature was favorable for the removal of oxygen-containing functional groups. The effect of methoxy groups on the HDO of these monomers was also investigated systematically. Basically, the conversion of multi-substituted monomers was tougher than that of mono-substituted ones, due to steric constraint and the inhibition of the electron-donating hydroxyl group. The selectivities to cyclohexane from phenol and anisole were improved significantly by increasing the temperature to 413 K. However, cyclohexanone was preferably produced over cyclohexane when using model compounds with multi-substituents (guaiacol and 2,6-dimethoxy-phenol), even at temperatures above 513 K. Comparative experiments were also conducted on the HDO of 1,2-cyclohexanediol with or without the presence of phenol, which clearly suggested that the further HDO of guaiacol and 2,6-dimethoxy-phenol was inhibited, probably due to the strong interactions between cyclohexanediol molecules and Brønsted acid sites.
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高影响因子
Reaction Kinetics Mechanisms and Catalysis
·
2018
·Vol. 124
·期 2
·587-601
·
IF 1.515
Wang Tao,Zhang Xinyu,Liu Jun,Liu Hanzi,Sun Baomin
NO conversion using dielectric barrier discharge (DBD) plasma with different catalysts was investigated. Cu/TiO 2 , Cu/Al 2 O 3 , Cu/ZSM-5, Cu/CNTs and Cu/AC catalysts were prepared by incipient wetness impregnation with 4 wt% Cu loaded. BET, SEM, XRD, XPS, H 2 -TPR, NH 3 -TPD were used to measure and evaluate various catalysts. Compared with the plasma only process, the combination of plasma with different catalysts significant improved the NO conversion rate. The highest NO conversion rate of 76% could be achieved with Cu/ZSM-5 catalyst at the energy density of 184 J/L. The results indicate that Cu species over the support ZSM-5 can produce high concentrations of CuO, largest specific surface area, largest amounts of acidity and lowest reduction temperature (209 °C), these factors conducive to plasma-catalyst reaction. Besides, Cu/TiO 2 and Cu/Al 2 O 3 catalysts exhibited excellent NO conversion at the low energy density because of the low thermal stability. After plasma-catalyst reaction, the reducibility of CuO and Cu 2 O over supports decreased and the peak position of acid sites shifted to lower temperature.
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高影响因子
ENERGY & FUELS
·
2022
·
IF 4.654
Jing Li,Suxia Ma,Rongji Cui,Nuo Xu,Ting Pei
Solid waste fly ash can encroach on land, contaminate the environment, and jeopardize human health. An alkali fusion method combined with a hydrothermal method was adopted to prepare fly ash-derived ZSM-5 molecular sieves, and a series of Fe/ZSM-5 catalysts were also prepared to test their catalytic performance in NO oxidation with H2O2. Moreover, the characteristics of catalysts synthesized by fly ash, as well as the relationship between the structure and the catalytic activity, were analyzed in detail through multiple characterization approaches. The results gave important insights into the active sites of catalytic oxidation. They concluded that 10% Fe/ZSM-5 had the best catalytic activity in which Fe sites and Brønsted acid sites collectively provided the catalytic functionality, and 92% NO removal efficiency was obtained achieved under optimal experimental conditions. This work provided a new way for recycling fly ash and developing low-cost catalysts for NO removal.
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高影响因子
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