JOURNAL OF APPLIED ELECTROCHEMISTRY
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2025
·Vol. 55
·期 6
·1557-1569
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IF 3
Zhang Xueni,Su Yuanzhang,Ao Debin,Hong Yan,Zou Min,Xie Jie,Zhou Qi,Wang Yuecong,He Wei,Chen Yuanming
A well-performing electrochemical immunosensor was prepared for detecting the tumor biomarker of carcinoembryonic antigen (CEA) by modifying a glassy carbon electrode (GCE) with a nanocomposite comprising gold nanoparticles (AuNPs), coiled carbon nanotubes (CCNTs), and chitosan (Chi) along with bovine serum proteins and CEA antibody. Various electrochemical methods such as cyclic voltammetry (CV) and square wave voltammetry (WV) were utilized for characterization. The substantial surface area and strong conductivity of AuNPs and CCNTs, and easy film-forming nature of Chi, enhanced the effective surface area and conductivity of the CEA-immunosensors to nearly twice that of the GCE before modification. Under the best experimental conditions, the CEA immunosensor showed good reproducibility with a selective linear detection range of 1 pg mL −1 –400 ng mL −1 and a detection limit as low as 1 pg mL −1 . The decrease in current exhibited a strong linear relationship with the logarithmic values of CEA concentration, boasting a correlation coefficient of 0.99379. The developed CEA immunosensor had good performance and the potential for practical application to real samples with good selectivity and immunity to other antigens in the presence of serum. Graphical abstract
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IONICS
·
2025
·
IF 2.6
Zhou Weijian,Luo Lingjie,Lin Weihao,Zheng Wei,Du Gaofeng,Wen Fan,Meng Fanbo
An increasing number of ore tailings generated from the metal ore beneficiation and smelting are expected to be reused in the near future; however, traditional treatments lack efficient secondary utilization of ore materials. Herein, polymetallic ore tailing is firstly recycled into SiO 2 -based nanofillers through a plasma-assisted ball milling technology, and the obtained SiO 2 is successfully applied in the PVdF-HFP-based solid electrolyte in Li metal batteries. It is found that the trace amounts of element Sn in ore tailing would be successfully introduced into the SiO 2 , and synthesized Sn-doped SiO 2 shows a lamellar structure with good crystallinity and rich oxygen vacancies. The addition of Sn-doped SiO 2 can improve the ionic conductivity, mechanical properties, and thermal stability of SSE, achieving low electrochemical polarization and high reversibility in the solid-state symmetrical Li cells. Manufactured solid-state NCM622||Li full cell exhibits good charge capability, stable cyclic property, and rate capability under room and high temperatures. This work is the first attempt to realize the recycling of ore tailings into SiO 2 nanofillers in advanced solid-state Li metal batteries, which may supply a new sight of the reusing treatment and applications of ore tailings in the field of electrochemical energy storage.
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JOURNAL OF MATERIALS SCIENCE
·
2025
·
IF 3.9
Gao Bingying,Sun Yunyun,Wang Guqi,Mao Linqiang
Hazardous formaldehyde and acetaldehyde removal remains a critical challenge in volatile organic compounds (VOCs) control technologies. Adsorption-based technologies offer distinct advantages for VOCs removal in practical applications due to their operational simplicity, energy efficiency, and adaptability to complex gas matrices. Although metal–organic frameworks (MOFs) exhibit superior adsorption capacity for VOCs compared to conventional adsorbents, their widespread industrial application has been hindered by high production costs and limited stability under humid conditions. This study prepared attapulgite (ATP)-functionalized MOF composites that synergistically enhanced both VOCs adsorption capacity at static (formaldehyde: 185 mg/g; acetaldehyde: 196 mg/g at RH = 80%) and dynamic (Breakthrough time for formaldehyde and acetaldehyde: 36 min and 30 min; penetrating adsorption capacity for formaldehyde and acetaldehyde: 2.126 mg/g and 5.258 mg/g), overcoming the intrinsic moisture sensitivity and scalability challenges of pristine MOFs for industrial VOCs abatement. Kinetic analysis confirms that VOCs adsorption on the ATP-MOF composites occurs primarily through spontaneous physical adsorption mechanisms. This study demonstrates an innovative strategy of hybridizing MOFs with natural ATP minerals, offering a viable pathway to enhance the practical deployment of MOF-based adsorbents in complex real-world environments.
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Scientific Reports
·
2025
·Vol. 15
·期 1
·1-13
·
IF 3.9
Zhang Yang,Huang Yu-Han,Wang Hou-Jie,Zheng Lin-Guo,Yang Qi-Yu,Wang Yan-Ru,Zhao Jiang,Jiang Cheng-Lu
This study primarily focuses on utilizing hair strands to achieve the crystallization of lithium carbonate, aiming to investigate the factors influencing crystal formation and volume variation by altering external conditions. To research the reason of crystallization, we have identified the following influencing factors: the direction of crystal growth, the temperature, the evaporation rate, selection of containers. The study ultimately revealed that the container material, solution temperature, evaporation rate, and crystal growth surface all influence crystallization and crystal volume.
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CELL DEATH AND DIFFERENTIATION
·
2025
·
IF 15.4
Yan Liping,Zhu Wenjie,Wang Mengqi,Zhao Ruinan,Zhang Guohao,Guo Xiangyu,Li Chunlan,Wang Suxia,Zhang Hui,Gao Peng
The role of autophagy in gastric cancer (GC) progression remains elusive, warranting further investigation into its mechanisms and therapeutic implications. In this study, we demonstrate that olfactory receptor family 2 subfamily T member 6 (OR2T6) is downregulated and correlates with poorer prognosis in GC tissues. Functionally, OR2T6 induces autophagy initiation while blocking autophagic flux by compromising lysosomal function and inhibits cell proliferation both in vitro and in vivo. Mechanistically, co-immunoprecipitation (Co-IP) and mass spectrometry (MS) analyses reveal that OR2T6 specifically binds to PPP3CA. OR2T6 facilitates the protein stability and enzyme activity of PPP3CA through the ubiquitin-proteasome system and the promotion of calcium ion influx via the Gs/cAMP/PKA channel signaling axis. Our further research demonstrates that OR2T6 binds to PPP3CA, which suppresses the AKT/mTOR signaling pathway, thereby inhibiting tumor proliferation and promoting autophagy initiation. Interestingly, it facilitates the nuclear translocation of TFEB, a key regulator of lysosomal biogenesis, which leads to the transcriptional inactivation of lysosomal target genes (LAMP1, MCOLN1, ATP6V1H, CTSB, and CTSD), impairing lysosomal function and blocking autophagic flux. Collectively, we report that OR2T6 binds to and promotes PPP3CA, which subsequently initiates autophagy, inhibits proliferation by suppressing the AKT/mTOR pathway, and then blocks autophagic flux through TFEB-mediated lysosomal dysfunction. These findings suggest that OR2T6 may be a potential therapeutic target for GC.
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MRS Communications
·
2025
·
IF 2.3
Hua Lingwen,Zhang Zhuoqing,Cao Lei,Li Huijie,Yang Rui,Zheng Weilin,Zhang Xing
Zirconium-doped SiOC (SiZrOC) ceramics with different Zr/Si molar ratios were fabricated through a thiol-ene cross-linking reaction followed by pyrolysis for high-temperature thermal insulation. The zirconium could be incorporated into the amorphous SiOC network when the Zr/Si molar ratio was below 0.25. The addition of zirconium can impede further decomposition of amorphous SiZrOC network, thereby significantly enhancing the high-temperature stability (above 1400°C). The SiZrOC-0.25 ceramics with octet truss structures were fabricated by digital light processing followed by pyrolysis at 1000°C, which exhibited a compressive strength of 6.42 ± 1.74 MPa even after heat treatment at 1400°C for 6 h under air atmosphere. The back temperature of the SiZrOC-0.25 ceramic was 205°C after exposure to 800°C for 20 min. Thus, the SiZrOC ceramics developed in this work are likely suitable for high-temperature thermal insulation, particularly in aerobic environments. Graphical abstract
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MICROCHIMICA ACTA
·
2025
·Vol. 192
·期 12
·830
·
IF 5.3
Hou Hongming,Long Jiajun,Li Xianglong
The chelation interaction between sodium lignosulfonate (SLS) and Cu 2+ is exploited to construct an enzyme-free creatinine sensing electrode (SLS-Cu/SPCE) via one-step electrodeposition on screen-printed carbon electrodes (SPCE). SLS chelation moderates Cu 2+ electro-reduction kinetics, resulting in finely structured Cu particles with a uniform and dense distribution. These Cu particles, with abundant unsaturated coordination sites, enhance creatinine adsorption and promote specific complexation with Cu 2+ , thereby increasing the oxidation current response for sensitive detection. The SLS-Cu/SPCE achieved a 0.12 µM detection limit, a 10–300 µM linear range, high reproducibility, and long-term stability over 28 days. Tests in human urine samples showed good agreement with enzymatic assays (relative error < 16%). The SLS-Cu 2+ coordination strategy offers a novel approach for optimizing Cu-based enzyme-free electrochemical creatinine sensors. Graphical A sodium lignosulfonate-Cu 2+ chelation strategy produces refined copper particles on SPCE with abundant unsaturated sites. These sites enhance creatinine adsorption and Cu 2+ complexation, enabling highly sensitive non-enzymatic detection (0.12 μM LOD, 10-300 μM range) with strong anti-interference and excellent stability.
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Nature Communications
·
2025
·Vol. 16
·期 1
·10365
·
IF 15.7
Qin Mengmei,He Meng,Cheng Chuanqi,Li Rui,Liu Cuibo,Zhang Bin
Electrocatalytic deuteron-dechlorination of trichloroacetic acid (TCAA) in D 2 O provides a green route to synthesize commercial acetic- d 3 acid- d (AA- d 4 ). Synthesizing AA- d 4 with a high Faradaic efficiency (FE) and reaction rate is highly challenging because of the difficult C−Cl bond deuteration of the 2-monochloroacetic-2,2- d 2 acid- d (MCAA- d 3 ) intermediate. Here, a quaternary ammonium salt surfactant-modified low-coordination copper electrocatalyst is designed, achieving TCAA-to-AA- d 4 with a 91% selectivity, 91% FE and 0.59 mmol h −1 reaction rate at −100 mA cm −2 . Mechanistic and kinetic studies reveal that the surfactant enhances the adsorption of MCAA- d 3 through electrostatic forces and increases the electron deficiency of Cu δ+ sites, which accelerates electron transfer and promotes C−Cl bond activation, increasing the AA- d 4 selectivity. Surfactant-induced low D 2 O coverage suppresses D 2 formation, improving the FE. AA- d 4 electrosynthesis (1.84 g) with a 30 mmol h −1 reaction rate and 65% FE at 600 mA cm −2 and deuterated drug applications demonstrate promising potential.
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MICROCHIMICA ACTA
·
2025
·Vol. 192
·期 12
·832
·
IF 5.3
Li Jiejun,Zhao Yixian,Huang Jiaxin,Wang Jiamin,Zhang Rongxia,Ni Meijun,Zhao Pengcheng,Fei Junjie,Xie Yixi
A highly sensitive electrochemical sensor for chlorogenic acid (CGA) detection has been developed using nitrogen-doped carbon nanorods loaded with NiCo bimetallic (NCNRs/NiCo). The composite material was synthesized through a pyrolysis strategy where NiCo-layered double hydroxide (LDH) was grown in situ on phenolic resin-derived carbon nanorods and subsequently carbonized. Material characterization confirmed the successful formation of uniformly dispersed NiCo nanoparticles anchored on nitrogen-doped carbon matrix, which provided abundant active sites. The NCNRs/NiCo-modified glassy carbon electrode (GCE) exhibited exceptional electrocatalytic activity toward CGA oxidation, achieving a wide linear detection range from 0.5 nM to 10 µM with an ultralow detection limit of 0.32 nM (S/ N = 3). The sensor demonstrated excellent selectivity against common interferents, good reproducibility and repeatability. Practical application in food samples showed satisfactory recoveries (97.6-104.1%) in good agreement with the ultraviolet-visible (UV-Vis) reference method. The outstanding performance originates from the synergistic effects between nitrogen-doped carbon framework and bimetallic nanoparticles, offering a promising platform for natural antioxidant determination in food quality control. Graphical
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BIOTECHNOLOGY LETTERS
·
2025
·Vol. 48
·期 1
·2
·
IF 2.1
Yang Xiaole,Tian Xia,Zhu Hai,Jia Weiwei,Li Jiahuan,Tang Ruizhou,Zhang Chuanzhi,Cao Yang,Li Tingting
Chiral amines, as essential chiral building blocks in drug synthesis, present a considerable challenge in biomanufacturing due to the requirement for highly efficient stereoselective synthesis. In this study, we successfully cloned and heterologously expressed a novel ( R )-amine transaminase, MagAT, from Mycolicibacterium agri . Systematic analysis showed optimal activity at pH 7.0, with the highest reaction rate occurring within 30 min at 50 ℃. However, considering overall thermal stability, 40℃ was selected as the operating temperature for subsequent experiments. Furthermore, the enzyme retained nearly 100% catalytic activity in the presence of 10% methanol, DMSO, and chloroform. Kinetic analysis demonstrated that MagAT possessed high substrate affinity, with Michaelis constants (K m ) of 5.13 ± 0.45 mM for ( R )-1-phenylethan-1-amine and 3.73 ± 0.34 mM for pyruvate. Substrate specificity studies indicated that MagAT efficiently catalyzed the conversion of C3-C8 aliphatic amines (e.g., ( R )-2-aminooctane) and monocyclic aromatic amines (e.g., ( R )-1-phenylethan-1-amine), whereas its catalytic activity significantly declined for sterically hindered substrates such as naphthylamine. 3D structural modeling with AlphaFold3 and molecular docking showed that steric hindrance from key residues in the O-pocket critically influences substrate specificity. This study not only provides an efficient biocatalyst for the biosynthesis of chiral amines but also establishes a theoretical basis for the rational design of transaminases with broader substrate adaptability.
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HEREDITAS
·
2025
·Vol. 162
·期 1
·232
·
IF 2.5
Xiaohui Yu,Jie Li,Jiangqiao Zhou
Background Coptisine (COP) is a natural compound extracted from Rhizoma Coptidis , and it represses the malignant biological behaviors of bladder cancer cells. However, the underlying molecular mechanism has not been fully elucidated. The aim of this study was to clarify the downstream mechanism by which COP treats bladder cancer. Materials and methods SwissTargetPrediction, STITCH, SymMap, ETCM, TCMSP, CTD databases were used to collect the related targets of COP. GeneCards, DisGeNET, TTD and OMIM databases were used to obtain the related targets of bladder cancer. A Venn diagram was used to identify the potential targets of COP in bladder cancer treatment. The protein-protein interaction network was constructed using STRING database, and Cytoscape 3.9.0 software was used to screen the hub targets. The binding relationship between COP and the hub targets was verified by molecular docking and molecular dynamics simulation. After the bladder cell lines T24 and BIU-87 were treated with different doses of COP, the regulatory effects of COP on PI3K/AKT pathway were investigated with western blotting. Additionally, the tumor-suppressive properties of COP on bladder cancer cells were validated with tumorigenesis model and metastasis model in nude mice. Results RAC-alpha serine/threonine-protein kinase 1 (AKT1), glycogen synthase kinase 3 beta (GSK3B), caspase-3 (CASP3), tumor necrosis factor (TNF) and cyclin D1 (CCND1) were identified as the main hub targets of COP in bladder cancer treatment. PI3K/AKT pathway was predicted to be a crucial pathway regulated by COP. The binding affinities between COP and AKT1, GSK3B, CASP3, TNF and CCND1 were high. COP treatment markedly repressed the phosphorylation level of ERK1/2, AKT1, PI3K p85 and mTOR in T24 and BIU-87 cells, and repressed the tumorigenesis and lung/liver metastasis of T24 cells in vivo. Conclusion COP may be a natural inhibitor for AKT1, GSK3B, CASP3, TNF and CCND1. COP represses PI3K/AKT pathway to suppress the progression of bladder cancer.
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JOURNAL OF FLUORESCENCE
·
2025
·
IF 3.1
Guan Chuheng,Jia Yumeng,Shi Zhongxiang,Wang Jing,Dong Yingnan,Wang Zhihui
The present paper details the preparation of CaAl 12- x O 19 : x Mn 4+ (CAO: Mn 4+ ) red phosphors based on CaAl 12 O 19 using a high-temperature solid-state method through Mn ion doping. A range of analytical techniques, including X-ray diffraction (XRD), scanning electron microscopy (SEM) and fluorescence spectroscopy, to comprehensively investigate the crystal structure, microstructure and photoluminescence characteristics of the samples. The results demonstrate that the doped Mn 4+ ions enter the octahedral sites of Al 3+ , and the lattice constants of the matrix does not change significantly because the ionic radius of the doped Mn 4+ ions is equivalent to the size of the Al 3+ ions. On this basis, the lattice size and lattice strain were calculated using the W-H equation. The results show that as the doping concentration gradually increases, both the lattice size and strain exhibit a slowly increasing trend. The prepared phosphor exhibited a polyhedral morphology, characterised by two hexagonal prisms with irregular hexagonal end faces, a substantial particle size, and optimal dispersion. When exposed to near ultraviolet light at a wavelength of 349 nm, the photoluminescence spectrum displays narrow-band emission characteristics, with the central wavelength of the emission peak at 654 nm and the full width at half maximum (FWHM) of 40.706 nm. The emission peak is attributed to the transition from 2 E g to 4 A 2g of Mn 4+ in a strong crystal field. With the increase of Mn 4+ doping concentration, the fluorescence intensity first increases and then decreases, and the fluorescence intensity is the highest when the doping concentration is 1.2 mol%. The analysis yielded three emission peaks, which were separated by the peak separation method. These peaks correspond to the emission of Mn 4+ at three different octahedral points of Al 3+ . The integrated area ratio of the three peaks indicates that Mn 4+ is predominantly present on the symmetrical sites. Thermal stability analysis demonstrates that the emission intensity of the phosphor decreases rapidly due to three factors. Firstly, the size of the Mn 4+ ions is smaller than the octahedral gap size. Secondly, the energy is higher. Thirdly, no radiation transition intensifies with the increase of temperature. The colour purity calculation indicates that the red phosphor prepared has a colour purity of over 99%. At the same time, the color temperature is below 6000k, which renders it suitable for indoor plant cultivation.
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