JOURNAL OF SEPARATION SCIENCE
·
2024
·Vol. 47
·期 11
·2400113
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IF 2.8
Masoumeh Firouzy,Alireza Ghiasvand,Payman Hashemi
An amide-based covalent organic framework (COF) was successfully synthesized using the reaction between 1,3,5-trimesoyl chloride and ethylenediamine. The structure and morphology of the COF were characterized using Fourier-transform infrared spectra, nuclear magnetic resonance spectroscopy, X-ray diffraction, scanning electron microscopy, energy-dispersive X-ray spectroscopy, and Brunauer-Emmett-Teller surface area analysis. The COF was employed as a solid-phase extraction adsorbent for the sampling and preconcentration of chlorophenols from industrial wastewater samples prior to high-performance liquid chromatography with ultraviolet detection. The experimental parameters influencing the extraction efficiency including type and volume of eluent solvent, sample solution volume, salt concentration, sample flow rate, and sample solution pH were investigated and optimized using a response surface methodology employing Box-Behnken-design. Under optimized conditions, calibration curves exhibited good linearities over the range of 0.003–10 µg/mL with determination coefficients (R 2 ) ranging from 0.9982 to 0.9999. The method's limits of detection ranged from 0.001 to 0.01 µg/mL. Good repeatability was achieved with relative standard deviations below 4.7%. The developed procedure utilizing the COF adsorbent was successfully applied to determine chlorophenols accurately and precisely in various industrial wastewater samples.
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AQUACULTURE
·
2025
·
IF 3.9
Yao Wang,Mengmeng Wang,Zhiyang Lin,Guangshuo Wang,Yunlong Qin,Mingzhu Liu,Fei Ling,Haifeng Jiang,Tianqiang Liu,Gaoxue Wang
Micropterus salmoides rhabdovirus (MSRV) is a highly pathogenic rhabdovirus that has caused significant economic losses in the aquaculture industry of largemouth bass. To date, there is no approved treatment for MSRV infection. In order to address this urgent need, virtual screening of the natural product monomer compound library targeting MSRV glycoprotein was conducted and the antiviral activity of 13 natural compounds identified in the screening was studied. Among these compounds, Aloe emodin (AE) exhibited high binding affinity and antiviral activity, inhibiting MSRV replication in grass carp ovary (GCO) cells with a half-maximal inhibitory concentration (IC 50 ) of 2.55 μM. Additionally, viral titers and cytopathic effects (CPE) were significantly reduced in the presence of AE. AE was demonstrated to directly destroy MSRV virions and inhibit their adsorption to GCO cells according to ultracentrifugation assay and viral binding assay. In vivo studies demonstrated that AE injection increased the survival rate of MSRV-infected largemouth bass by 63.3 %, which was 46.7 % higher than that of the virus group. RT-qPCR and pathological tissue section results demonstrated that AE treatment could significantly reduce viral loads in the liver, spleen, and kidney on days 1, 4, and 7 post-infection, and AE effectively mitigated tissue damage caused by MSRV infection. Circular dichroism (CD) spectroscopy and Isothermal titration calorimetry (ITC) showed that AE induced changes in the secondary structure of the MSRV glycoprotein and exhibited high affinity for the glycoprotein (Kd = 2.803 × 10 −5 M, ΔG = −25.99 kJ/mol). These results suggested that AE was a natural product with potent anti-MSRV activity through direct targeting of the glycoprotein.
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PHYSICS OF FLUIDS
·
2024
·Vol. 36
·期 1
·
IF 4.1
Yu Jia-Jia,Jiang Lu-Yang,Huang Li,Li Gu-Yuan,Bake Maitiniyazi,Li You-Rong
Hydrodynamic cavitation, which occurs when the local pressure is below the saturated vapor pressure in hydrodynamic flow, is ubiquitous in fluid dynamics and implicated in a myriad of industrial and biomedical applications. Although extensively studied in isotropic liquids, corresponding investigations in anisotropic liquids are largely lacking. In this paper, the hydrodynamic cavitation in the bluff body bypass flow of nematic liquid crystal 5CB in the microchannel is experimentally investigated. By 5CB, we mean the thermotropic liquid crystal 4′-pentyl-4-biphenylcarbonitrile. When the Reynolds number is in the range of 3 × 10 −4 < Re < 1.2 × 10 −3 , a special flow phenomenon behind the bluff body is observed, namely, the disclination loop. The critical Reynolds number of cavitation inception varies with the shape of the bluff body, while the lowest value corresponds to the triangular bluff body. The hydrodynamic cavitation occurs in the Stokes flow regime with the Reynolds number significantly lower than 0.1 for all bluff bodies. There is a close relation between the oscillation behavior of cavitation domains and the structure of the bluff body. In addition, the pressure difference between the inlet and outlet of the microchannel shows linear relation with the Reynolds number rather than the quadratic relation for isotropic fluids, which proves the presence of shear thinning in the flow of nematic liquid crystals. The study in this paper on the hydrodynamic cavitation of nematic liquid crystal can broaden the research on providing new approaches for the enhancement of fluid mixing and heat transfer in microfluidic chips.
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LWT-FOOD SCIENCE AND TECHNOLOGY
·
2024
·
IF 6
Qian Zhang,Lin Li,Yu-Qiao Wang,Hai-Tao Wu,Bei-Wei Zhu
A water-in-oil-in-water (W/O/W) emulsion for the encapsulation of salidroside (Sal) was constructed. This study aimed to detect the impact of whey protein isolate (WPI) on the microstructure and stability of Sal W/O/W emulsion, which was prepared by two-step emulsification. First , Sal and sodium alginate (SA) were added to the inner water phase, and polyglycerol polyricinoleate (PGPR) was added to the oil phase to prepare a water-in-oil (W/O) emulsion. Second , different concentrations of WPI were added to the outer water phase containing SA to prepare a Sal W/O/W emulsion. The microstructure, stability, moisture distribution, rheological properties, and encapsulation efficiency of Sal were detected. The results showed that the particle size and the size distribution range became smaller and narrower with increasing WPI concentration . When the concentration of WPI was greater than 2%, the double-layer structure remained stable over 28 days of storage at 4 °C. Moreover, when the WPI concentration reached 4%, the entrapment efficiency of Sal was the highest. These results indicated that the optimum concentration of WPI was 4%.
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Foods
·
2024
·Vol. 13
·期 10
·1561
·
IF 4.7
Qiaoyi Zhou,Jinjing Gao,Xueyan Sun,Yicheng Liang,Minqi Ye,Dongxia Liang,Caijin Ling,Binghu Fang
Tea plants have a long cultivation history in the world, but there are few studies on polysaccharides from fresh tea leaves. In this study, tea polysaccharides (TPSs) were isolated from fresh tea leaves. Then, we investigated the characteristics of TPSs during in vitro simulated digestion and fermentation; moreover, the effects of TPSs on gut microbiota were explored. The results revealed that saliva did not significantly affect TPSs’ molecular weight, monosaccharide composition, and reducing sugar content, indicating that TPSs cannot be digested in the oral cavity. However, TPSs were partially decomposed in the gastrointestinal tract after gastric and intestinal digestion, resulting in the release of a small amount of free glucose monosaccharides. Our in vitro fermentation experiments demonstrated that TPSs are degraded by gut microbiota, leading to short-chain fatty acid (SCFA) production and pH reduction. Moreover, TPSs increased the abundance of Bacteroides, Lactobacillus, and Bifidobacterium but reduced that of Escherichia, Shigella, and Enterococcus, demonstrating that TPSs can regulate the gut microbiome. In conclusion, TPSs are partially decomposed by gut microbiota, resulting in the production of SCFAs and the regulation of gut microbiota composition and function. Therefore, TPSs may be used to develop a prebiotic supplement to regulate the gut microbiome and improve host health.
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BMC Oral Health
·
2024
·Vol. 24
·期 1
·1-12
·
IF 2.6
Li Wei,Jiao Ruofeng,Luo Shiyi,Liu Zefei,Song Jukun,Chen Zhu
Objective Explore the therapeutic mechanism of Coptidis Rhizome (CR) in periodontitis using network pharmacology, and validate it through molecular docking and in vitro experiments. Methods Screened potential active components and target genes of CR from TCMSP and Swiss databases. Identified periodontitis-related target genes using GeneCards. Found common target genes using Venny. Conducted GO and KEGG pathway analysis. Performed molecular docking and in vitro experiments using Berberine, the main active component of CR, on lymphocytes from healthy and periodontitis patients. Assessed effects on inflammatory factors using CCK-8, flow cytometry, and ELISA. Results Fourteen active components and 291 targets of CR were identified. 30 intersecting target genes with periodontitis were found. GO and KEGG analysis revealed oxidative stress response and IL-17 signaling pathway as key mechanisms. Molecular docking showed strong binding of Berberine with ALOX5, AKT1, NOS2, and TNF. In vitro experiments have demonstrated the ability of berberine to inhibit the expression of Th17 + and other immune related cells in LPS stimulated lymphocytes, and reduce the secretion of IL-6, IL-8, and IL-17. Conclusion CR treats periodontitis through a multi-component, multi-target, and multi-pathway approach. Berberine, its key component, acts through the IL-17 signaling pathway to exert anti-inflammatory effects.
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MOLECULES
·
2024
·Vol. 29
·期 8
·1703
·
IF 4.2
Meihua Liang,Yong Yan,Jiaxuan Yang,Xiaodong Liu,Rongrong Jia,Yuanyuan Ge,Zhili Li,Lei Huang
Microelectromechanical systems (MEMS) gas sensors have numerous advantages such as compact size, low power consumption, ease of integration, etc., while encountering challenges in sensitivity and high resistance because of their low sintering temperature. This work utilizes the in situ growth of Zeolitic Imidazolate Framework-8 (ZIF-8) followed by its conversion to N-doped ZnO. The results obtained from scanning electron microscopy (SEM) and transmission electron microscopy (TEM) indicate that the in situ derivation of ZIF-8 facilitates the adhesion of ZnO particles, forming an island-like structure and significantly reducing the interfaces between these particles. Furthermore, powder X-ray diffraction (XRD) analysis, elemental mapping, and X-ray photoelectron spectroscopy (XPS) analysis confirm the conversion of ZIF-8 to ZnO, the successful incorporation of N atoms into the ZnO lattice, and the creation of more oxygen vacancies. The ZIF-8-derived N-doped ZnO/MEMS sensor (ZIF (3)-ZnO/MEMS) exhibits remarkable gas sensitivity for ethanol detection. At an operating temperature of 290 °C, it delivers a substantial response value of 80 towards 25 ppm ethanol, a 13-fold enhancement compared with pristine ZnO/MEMS sensors. The sensor also exhibits an ultra-low theoretical detection limit of 11.5 ppb to ethanol, showcasing its excellent selectivity. The enhanced performance is attributed to the incorporation of N-doped ZnO, which generates abundant oxygen vacancies on the sensor’s surface, leading to enhanced interaction with ethanol molecules. Additionally, a substantial two-order-of-magnitude decrease in the resistance of the gas-sensitive film is observed. Overall, this study provides valuable insights into the design and fabrication strategies applicable to high-performance MEMS gas sensors in a broader range of gas sensing.
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CHEMICAL ENGINEERING JOURNAL
·
2024
·
IF 13.3
Xiaodan Yu,Rongzheng Ren,Chunming Xu,Jinshuo Qiao,Wang Sun,Kening Sun,Zhenhua Wang
The cathode of a solid oxide fuel cell (SOFC) must exhibit both high activity and robust durability for effective utilization in electrochemical oxygen reduction reaction (ORR). To address this challenge, we present a novel strain engineering strategy that involves the creation of nanoscale local lattice strain microdomains to further enhance the ORR kinetics. Specifically, Ga cations are introduced into some of the B-sites of the perovskite Pr 0.4 Sr 0.6 Fe 0.5 Co 0.5 O 3-δ (PSFC). Benefiting from local lattice strain engineering, the bulk oxygen migration coefficient of the locally strained PSFC sample is significantly enhanced, reaching twice that of the pure PSFC sample at 650 °C. Moreover, the polarization impedance of PSFC (0.102 Ω·cm 2 ) is more than twice that of Pr 0.4 Sr 0.6 Fe 0.4 Co 0.5 Ga 0.1 O 3-δ (PSFCG, 0.043 Ω·cm 2 ) at 800 °C. Microscopic structural analyses and computational calculations indicate that the optimized electronic structure of the rich micro-strain catalyst reduces the bond energy of adjacent B-O bonds and the oxygen transport barrier. This work demonstrates a practical local lattice micro-strain engineering strategy and provides a new approach for improving the performance of ORR electrocatalysts.
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ANALYTICAL CHEMISTRY
·
2024
·
IF 6.7
Cheng Qu,Yuchuang Geng,Zhongxiang Ding,Yuzhu Li,Hao Jiang,Mengke Su,Honglin Liu
A feedback inhibition effect of high autoinducer levels on metabolite secretion in Chromobacterium subtsugae (C. subtsugae) was evidenced by in situ spatiotemporal surface-enhanced Raman spectroscopy (SERS) profiling. The hierarchical hydrophobic plasmonic array in agar medium is structured by oil/water/oil (OL/W/OH) triphasic interfacial self-assembly. The hydrophobic layer acts as a “door curtain” to selectively permit adsorption of a quorum sensing (QS)-regulated fat-soluble metabolite, i.e., violacein (Vio), and significantly blocks nonspecific adsorption of water-soluble proteins, etc. The SERS profiling clearly evidences that the diffusion of N-hexanoyl-l-homoserine lactone (C6-HSL) in agar medium quickly triggers the initial synthesis of Vio in C. subtsugae CV026 but surprisingly inhibits the intrinsic synthesis of Vio in C. subtsugae ATCC31532. The latter negative response might be related to the VioS repressor of ATCC31532, which negatively controls violacein production without influencing the expression of the CviI/R QS system. Moreover, two sender–receiver systems are constructed by separately coculturing CV026 or ATCC31532 with Hafnia alvei H4 that secretes large amounts of C6-HSL. Expectedly, the cocultivation similarly triggers the initial synthesis of Vio in CV026 but seems to have a quite weak negative effect on the intrinsic synthesis in ATCC31532. In fact, the negative regulation in ATCC31532 might be affected by a diffusion-dependent concentration effect. The H4 growth and its secretion of C6-HSL are a slow and continuous process, thereby avoiding the gathering of local high concentrations. Overall, our study put forward an in situ SERS strategy as an alternative to traditional bioluminescent tools for highly sensitively analyzing the spatiotemporal communication and cooperation in live microbial colonies.
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DESALINATION
·
2024
·
IF 8.3
Zhongsen Yan,Ziyue Zhang,Yaman Huang,Haiqing Chang,Dan Qu,Yuling Jiang,Hongting Wan,Senlin Shao,Fangshu Qu
Membrane scaling and subsequent membrane wetting pose significant impediments to the application of membrane distillation (MD) in treating high-salinity wastewater. This study introduces an innovative in-situ bubble cleaning strategy for the amelioration of membrane scaling in MD processes. A catalytic membrane was fabricated by introducing MnO 2 as a catalyst by electrospinning and the self-cleaning mechanism involving micro-nano bubbles and superhydrophobic surface was systematically investigated. Results showed that the fabricated MnO 2 /PVDF nanofiber membranes (M-FMP) showed superhydrophobic properties, with a water contact angle and rolling angle of 158° and 17°, which significantly improved the membrane's resistance to wetting and fouling. The M-FMP membrane exhibited exceptional performance across ten experimental cycles, achieving a flux recovery rate of over 92 % and demonstrating robust chemical stability following repeated cleanings with 0.006 wt% H 2 O 2 . Notably, CaSO 4 deposition on the M-FMP membrane was approximately 46.0 μg/cm 2 , accounting for only 14.6 % of the deposition observed on the M-P electrospun membrane. The in-situ cleaning process leveraging micro-nano bubbles proved to be significantly more effective than conventional ex-situ cleaning methods, offering superior performance in removing scale deposits. Overall, this pioneering in-situ catalytic self-cleaning approach offers a new option for controlling MD membrane scaling.
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WATER RESEARCH
·
2024
·
IF 11.4
Wei Chen,Peng Cui,Lian Zhou,Han Zheng,Xude Zhao,Guansheng Liu,Juntao Zhang,Hua Zhong
In this study, column experiments were conducted to explore on the method of emplacement of magnetite nanoparticles (MNPs) for in situ activation of persulfate (PS) in sand porous media to degrade 1,2-dichloroethane (DCA), a typical recalcitrant chlorinated compound. Different molar ratios between PS and DCA (0:1, 2:1, 5:1 and 20:1) and mass contents of MNPs in the sand (0 %, 1.9 % and 5.4 %) were tested. In the absence of MNPs, degradation of DCA was negligible for a hydraulic retention time of 7 h. Presence of MNPs at the content of 1.9 % enhanced degradation of DCA and the highest removal efficiency (34.2 %) was observed at the PS-to-DCA molar ratio of 5:1. At the MNPs content of 5.4 %, increase of the PS-to-DCA molar ratio from 2:1 to 20:1 lead to not only increase in DCA removal efficiency but also substantial enhancement in chloride production, indicating that high PS concentration could cause significant degradation of the Cl-containing intermediates. In contrast to MNPs, Fe 3 O 4 solids with much larger size (∼1 μm) were much less effective in activating PS for DCA removal even at a significantly higher content in the medium. The transport data could be well fitted by the one-site chemical nonequilibrium model, which showed kinetic DCA sorption to the MNPs as a key process for the transport. In the long-term injection experiment, a stable and significant removal of DCA (∼50 %) was observed for 254 days at the MNP content of 1.9 %. The results of this study show the potential of MNPs as a sustainable PS activator in injection-based in situ chemical oxidation for groundwater remediation.
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JOURNAL OF AGRICULTURAL AND FOOD CHEMISTRY
·
2024
·
IF 5.7
Lingjuan Tang,Zhiyao Zhang,Ling Sun,Xu Gao,Xinyue Zhao,Xinru Chen,Xingyu Zhu,Aixue Li,Lijun Sun
Abscisic acid (ABA) plays an important regulatory role in plants. It is very critical to obtain the dynamic changes of ABA in situ for botanical research. Herein, coupled with paper-based analysis devices, electrochemical immunoelectrodes based on disposable stainless steels sheet were developed for ABA detection in plants in situ. The stainless steel sheets were modified with carbon cement, ferrocene-graphene oxide-multi walled carbon nanotubes nanocomposites, and ABA antibodies. The system can detect the ABA in the range of 1 nM to 100 μM, with a limit of detection of 100 pM. The ABA content in tomato leaves under high salinity was detected in situ. The trend of ABA changes was similar to the expression of SlNCED1 and SlNCED2. Overall, this study offers an approach for in situ detection of ABA in plants, which will help to study the regulation mechanism of ABA in plants and to promote the development of precision agriculture.
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