JOURNAL OF LUMINESCENCE
·
2023
·
IF 3.6
Zhaofa Li,Quanxiu Zhou,Palanivel Sathishkumar,Liang Peng,Qunfang Wang,Feng Long Gu
The present work fabricated a novel dual-emission responsive ratiometric fluorescent hybrid nanoprobe using a mixture of blue fluorescence-emitting N-Doped Carbon Quantum Dots (N-CQDs) and red fluorescence-emitting l -cysteine-capped CdSe/ZnS Quantum Dots (Lcys-CdSe/ZnS QDs). The addition of Cu 2+ resulted in the strong and specific fluorescence quenching in Lcys-CdSe/ZnS QDs while N-CQDs retained their fluorescence intensity. Further exploration of the nanoprobe showed that the detection limit was 3.78 × 10 −9 M and the linear range was below 3.2 × 10 −6 M. The nanoprobe possessed excellent anti-interference in the Cu 2+ determination and its application was successful in natural water samples. Experimental results suggested that the superficial left cysteine (Lcys) combines with Cu 2+ causing static quenching. Results of density functional theory (DFT) computation explained that Cu 2+ has the greatest possibility to combine with surficial Lcys and furthermore illustrated the role of the combination in static quenching. The work showed one of origin of the selectivity of the nanoprobe and a potentially guidance to design high selective ratiometric fluorescent nanoprobes.
✓ 已验证
产品关联
DOI
高影响因子
PHARMACEUTICAL DEVELOPMENT AND TECHNOLOGY
·
2022
·
IF 3.915
Qingfa Guo,Fanyuan Kong,Xiaoru Pan,Shuhua Cao
Recent findings revealed that low-concentration paclitaxel(DTX) could enhance cytotoxicity by upregulating p53 expression in lung cancer cell lines. So, co-delivery of DTX and RFP-p53 gene with PEA nanoparticles (NPs) was studied. The prepared DTX loaded PEA NPs (PEA/DTX) were characterized by particle size distribution, morphology, zeta potential, and crystallography and cytotoxicity. Results showed that the PEA/DTX NPs had a mall particle size (≤100 nm), moderate zeta potential (≥40 mV) and drug loading of 9.0%, DTX was released from PEA/DTX NPs in an extended period in vitro. More important, agarose gel electrophoresis showed that PEA/DTX cationic NPs were able to completely bind RFP-p53 gene with mean particles size and zeta potential. Studies on cellular uptake of (PEA/DTX)/RFP-p53 NPs demonstrated that both drug and gene were effectively taken up by A549 tumor cells. It was found that intravenous injection of (PEA/DTX)/RFP-p53 NPs efficiently inhibited growth of subcutaneous A549 carcinoma in vivo (p < 0.05) and was significantly less side effect than that of mice treated with the other groups. Therefore, the (PEA/DTX)/RFP-p53 NPs might be a promising candidate for A549 cancer therapy.
✓ 已验证
产品关联
DOI
PubMed
高影响因子
PROCESS BIOCHEMISTRY
·
2023
·
IF 4.4
Xiaoxuan Cai,Miaoen Huang,Xixiang Huang,Huan Liu,Tianji Wang,Li Li,Weiguang Yang,Hui Luo,Yingnian Lu
The organism Sipunculus nudus L is a good resource for marine peptides because of its rich protein. However, the disadvantage of biological peptides is their short metabolic half-life, therefore, structural modification of peptides is necessary. In this research, the enzymatic hydrolysis of Sipunculus nudus L was performed using trypsin and then peptides were isolated and purified from the hydrolysates, four novel tripeptides were identified by LC-MS-MS as Ser-Arg-Pro (SRP, m/z = 358.39), Pro-Arg-Pro (PRP, m/z = 368.43), Arg-Pro-Ala (RPA, m/z = 342.39), and Lue-Pro-Lys (LPK, m/z = 356.46), their ACE inhibitory activity was investigated with IC 50 value as following 0.046, 0.42, 0.97, and 6.54 mM, respectively. Because of its highest ACE inhibitory activity, SRP was further selected to modify the chemical structure for a sustained release agent DSPE-PEG-SRP. Compared with 7 h of free SRP, the release of DSPE-PEG-SRP in vitro was approximately 120 h. The ACE inhibition rate of SRP released from the synthetic product and the raw material SRP was almost the same, molecular docking demonstrated that ACE inhibitory activity was attributed to the formation of hydrogen bonds between SRP and ACE active sites . In conclusion, DSPE-PEG-SRP, a novel sustained release agent, has great potential and is expected to be further applied to treat hypertension.
✓ 已验证
产品关联
DOI
高影响因子
ACS Biomaterials Science & Engineering
·
2023
·
IF 5.8
Can Cheng,Heng Li,Jingwen Liu,Liang Wu,Zhengdong Fang,Geliang Xu
Tissue engineering approaches such as the electrospinning technique can fabricate nanofibrous scaffolds which are widely used for small-diameter vascular grafting. However, foreign body reaction (FBR) and lack of endothelial coverage are still the main cause of graft failure after the implantation of nanofibrous scaffolds. Macrophage-targeting therapeutic strategies have the potential to address these issues. Here, we fabricate a monocyte chemotactic protein-1 (MCP-1)-loaded coaxial fibrous film with poly(l-lactide-co-ε-caprolactone) (PLCL/MCP-1). The PLCL/MCP-1 fibrous film can polarize macrophages toward anti-inflammatory M2 macrophages through the sustained release of MCP-1. Meanwhile, these specific functional polarization macrophages can mitigate FBR and promote angiogenesis during the remodeling of implanted fibrous films. These studies indicate that MCP-1-loaded PLCL fibers have a higher potential to modulate macrophage polarity, which provides a new strategy for small-diameter vascular graft designing.
✓ 已验证
产品关联
DOI
PubMed
高影响因子
International Journal of Electrochemical Science
·
2023
·
IF 1.5
Yao Wang,Dan Li,Junfeng Kang,Shiye Guan,Daoxin Wu
In this work, graphene (GR) and Ag-ZnO nanocomposites were prepared and used together with methylene blue (MB) as electrode materials to modify a glassy carbon electrode (GCE). The electrochemical behavior of L-tryptophan (L-Trp) on the fabricated MB/Ag-ZnO/GR/GCE was investigated by cyclic voltammetry and differential pulse voltammetry. MB/Ag-ZnO/GR clearly enhanced the electrochemical oxidation of L-Trp. In a 0.1 mol/L phosphate buffer solution at pH 4.0, the peak current response at the modified electrode increased linearly with an increase in the L-Trp concentration from 2.0 x 10 -6 to 1.4 x 10 -4 mol/L, and the detection limit (S/N = 3) was 1.0 × 10 -6 mol/L. The electrode showed good reproducibility, stability, and selectivity. The sensor was used for the determination of L-Trp in biofluid samples (pig serum and pig urine), and the recovery rate ranged from 97.1% to 104.2%. The fabricated electrode was proposed for the electrochemical analysis of L- Trp, indicating the potential for practical applications in the biological field.
✓ 已验证
产品关联
DOI
高影响因子
PROGRESS IN ORGANIC COATINGS
·
2023
·
IF 6.6
Yonghong Pan,Daofeng Zhou,Tingting Cui,Yu Zhang,Lei Ye,Ye Tian,Ping Zhou,Yi Liu,Hidetoshi Saitoh,Botao Zhang,Hua Li
The diversity of medical application scenarios demands fine-tuning of drug release profiles for better patient care. Here we report a facile strategy to construct composite coatings as universal drug delivery systems with tunable release efficacy. A poly-L-lactic acid (PLLA)-polycaprolactone (PCL) coating was fabricated by liquid flame spray and its performance was verified by loading chloramphenicol (CAM) as bactericidal component. Physiochemical analyses revealed the homogeneity of chemistry and integrity of functional groups of the composite coatings. Release of CAM was enhanced by the increase of the PCL content in the composite coatings, showing a controllable manner. Kinetics analysis suggested the release mechanism of Korsmeyer-Peppas model for both the PLLA-CAM and the PCL-CAM coatings, and the release regime for the PLLA-PCL composite coatings transited from the Higuchi model to the Korsmeyer-Peppas model when the PCL content increased from 30 % to 70 %. Further antibacterial testing revealed tailorable antibacterial activity of the coatings against both Escherichia coli and Staphylococcus aureus . Regulating the release of the drug CAM through altering PCL content in the PLLA-PCL coating would give inspiring insight into green fabrication of polymeric coatings with tunable drug release for versatile applications.
✓ 已验证
产品关联
DOI
高影响因子
PHYSIOLOGICAL AND MOLECULAR PLANT PATHOLOGY
·
2023
·
IF 2.7
Jiaoli Huang,Sisi Liu,Rukuan Liu,Youjin Yi,Changzhu Li,Zhihong Xiao,Jia Tu,Jingjing Xiao
Although Litsea cubeba essential oil (LCEO) is widely used for against fungi, antifungal mechanism of it is not fully understood. In the study, antifungal potential and mechanism of LCEO on Colletotrichum scovillei , the main pathogen fungi caused pepper anthracnose , were studied. Results of gas chromatograph-mass spectrometry (GC-MS) showed that citral (67.37%) and d -Limonene (11.72%) were the major components of LCEO. LCEO was effective in inhibiting germination of C. scovillei spores , and the minimum inhibitory concentration (MIC) was 0.60 mg mL −1 . To determine influence of LCEO on cell membrane integrity and permeability of C . scovillei , SEM observation, OD 260 nm (nucleic acids), OD 280 nm (proteins), extracellular electric conductivity , cell membrane permeability (propidium iodide, PI), cell wall integrity (calcofluor white, CFW), malondialdehyde (MDA) content and ergosterol content were analyzed. Spores exposed to LCEO were partly deformed, collapsed, shrunken and shriveled. The OD 260 nm, OD 280 nm and the extracellular electric conductivity of LCEO treatment group increased by 2.0, 1.5 and 1.2 times, respectively, at 12 h after treatment of LCEO (0.6 mg mL −1 ). Besides, high accumulation of MDA content indicated that LCEO significantly enhanced membrane lipid peroxidation . Moreover, LCEO induced a decrease in ergosterol content of C. scovillei . These findings suggest that LCEO exhibited potent inhibitory activities against C. scovillei , and the cell membrane permeability and cell wall integrity of C. scovillei treated by LCEO were significantly destroyed.
✓ 已验证
DOI
高影响因子
Horticulturae
·
2023
·Vol. 9
·期 7
·789
·
IF 3.1
Tao Xu,Min Zhang,Tianchi Chen,Lili Gong,Lingling Hu,Jie Yang,Haoxuan Si,Yueyan Wu
Suboptimal light stress in grapevines is increasing worldwide with the spread of grapevine cultivation, which can affect grapevine physiology and productivity, such as in Southern China. Abscisic acid (ABA) is an important hormone in plant adaptive responses to abiotic stress, including low light stress. However, ABA signaling pathway genes (APGs) are not well characterized in the grapevine, and little is known of their potentially mitigating role in grapevine growth under weak light stress. Our study aimed to explore the potential role of the ABA signaling pathway in the response of grapevines to suboptimal light conditions. In this study, APGs were identified in the grapevine genome, and the distribution of conserved motifs was shown to reflect their phylogenetic relationships. Gene duplication analysis indicated that segmental duplication was an important driver for gene expansion in the grapevine ABA signaling pathway. Suboptimal conditions of light were shown to seriously affect the growth of grapevine leaves and berries, with the differential regulation of APGs in the grapevine. Our study summarizes the basic characteristics of APGs in grapevine, which can now be examined further for their roles in grapevine’s response to suboptimal light conditions.Keywords:grape;weak light stress;abscisic acid;gene family
✓ 已验证
产品关联
DOI
高影响因子
FOOD CHEMISTRY
·
2023
·
IF 8.8
Run Ji,Xiuling Zhang,Chenghai Liu,Wentao Zhang,Xiaofeng Han,Hengtian Zhao
The work within this study aimed to investigate and compare the effects of compound enzyme extraction (CE), ultrasonic chemical extraction (UC) and combined fermentation extraction (CF) on the physicochemical properties, microstructure, and functional properties of soluble dietary fiber (SDF) extracted from blue honeysuckle berries. The results showed that CE-SDF had higher crystallinity (32.41%). UC-SDF had the highest yield (13.32 ± 0.80 g/100 g). CF-SDF had the maximum inhibition of α-amylase (50.82 ± 0.76%) and α-glucosidase (54.87 ± 1.25%). The in vitro hypoglycemic activity of the three SDFs was observed in the order of CF > CE > UC. Meanwhile, the purity of SDF had a strong positive correlation with its antioxidant and in vitro hypoglycemic capacities. The crystallinity of SDF was found to be positively correlated with its molecular weight and thermal properties. Additionally, the sugar composition of SDF was found to be an important factor affecting its biological activity.
✓ 已验证
产品关联
DOI
PubMed
高影响因子
JOURNAL OF MATERIALS SCIENCE-MATERIALS IN ELECTRONICS
·
2023
·Vol. 34
·期 17
·1-11
·
IF 2.8
Liu Ji,Du Haiying,Jiang Yuqiu,Zhang Lanying,Ma Yanqi,Fu Bingjie,Hu Yao,Kang Heng
Artificial sweetener needs to control the maximum dosage, such as aspartame, cyclamate, saccharin sodium, and sucralose, are mainly used in fruits, jams, beverages, desserts, and dairy products. In this research, a novel label-free fluorescent sensor based on carbon quantum dots (CQDs) were prepared by a one-step hydrothermal method using Eupatorium odoratum L . , as a highly sensitive “turn-on”-type fluorescent probe for the detection of artificial sweetener. They exhibit photoluminous emission at 444 nm under 370 nm excitation, with a quantum yield of 20.36%. Under optimal conditions, the standard curve of aspartame, neotame, and sucralose had good linearity in the concentration range of 0–750 µmol/L, 0–400 µmol/L, and 100–800 µmol/L, respectively, and the linear correlation coefficients were 0.9913, 0.9978, and 0.9974. The CQDs were also shown to selectively react with aspartame, neotame, and sucralose, leading to fluorescence enhancing effect, and low limits of detection were 4.2383 µmol/L, 6.8834 µmol/L, and 7.6576 µmol/L, respectively, which were much lower than the limits of detection for aspartame (1.0194–33.9789 mmol/L), neotame (26.4229–792.6861 µmol/L), and sucralose (0.3772–12.5745 mmol/L) in various foodstuffs as stipulated in GB 2760-2014. The results indicated our developed sensor based on E. odoratum L . CQDs has great application prospects for artificial sweeteners detection.
✓ 已验证
产品关联
DOI
高影响因子
JOURNAL OF MOLECULAR LIQUIDS
·
2022
·
IF 6.633
Lin Chen,Li Ge,Qinghui Liang,Zhenbo Zhao,Kedi Yang
The enantioseparation of valsartan enantiomers using chiral eutectic solvents (ESs) as extractants was initially studied. The hydrophobic ESs (LM-DTTA) formed by L-menthol and (+)-Di-p-toluoyl-D-tartaric acid is a suitable chiral medium for the enantioselective extraction of R- and S-valsartan. Four principal parameters affecting chiral extraction, such as phase volume ratio, initial rac -valsartan concentration, extraction temperature, and system pH, were evaluated. The results showed that LM-DTTA has better and higher enantioselectivity levels, and one-step chiral extraction achieved an ee value of S-valsartan up to 91% under the optimized conditions. In addition, this chiral medium can be recycled through simple back-extraction with sodium bicarbonate solution and reused at least five times, giving similar results. This work provides a clear example of enantioseparation of the rac -valsartan via liquid–liquid enantioselective extraction using the LM-DTTA as a novel chiral recognition and separation medium. As a result, the established enantioselective extraction method can efficiently separate the R- and S-enantiomer and promote sustainable development for the production of S-valsartan.
✓ 已验证
产品关联
DOI
高影响因子
FOOD HYDROCOLLOIDS
·
2022
·
IF 11.504
Na Zhang,Xue Han,Rumeng Yu,Xiaomin Tang,Bo Zhang,Yanyan Zhang,Xiaoyu Yin,Wenrui Ni,Mengze Wang,Bolin Zhang,Junfeng Fan
A bile salt (BS)-stabilized water/oil interface is a prerequisite for the pancreas lipase (PL) mediated hydrolyzation of triacylglycerol. Previous fat digestion research mainly focused on direct lipase inhibition by bioactive substances while ignoring the destructive role of these substances on BS-stabilized lipid emulsions . Here, we showed that polysaccharides (LP) weakly suppressed PL but destroyed BS-stabilized emulsions and thus inhibited free fatty acid (FFA) release. Physicochemical analysis showed that LP actions toward emulsion stability were mainly due to endogenous minerals (28%) in LP which affected aggregation and amphiphilic properties of BS-polysaccharide composites. This was evidenced by high wettability and low interfacial tension in demineralized polysaccharide and BS composites, with three phase contact angles which were identified as 80.4° and 95.6° as well as interfacial tensions as 9.26 and 9.37 mN/m, respectively. However, the low wettability, which was detected as 76.2° and 78.6° as well as high interfacial tension as 10.77 and 11.02 mN/m, respectively, was only observed in mineral-containing polysaccharides and BS composites. Further investigations showed that interactions with mineral-containing polysaccharides occupied hydrophobic sites in BS thereby elevating composite hydrophilicity. These findings provided novel insights into the effects of plant-sourced polysaccharides on fat digestion from an interface chemistry perspective, and in particular, they deepened our understanding of polysaccharide interactions with intestine-secreted lipid digestants.
✓ 已验证
产品关联
DOI
高影响因子
危险品化学品经营许可证（带存储）