ACS Nano
·
2017
·
IF 13.942
Xiao Sun,Ruohong Du,Li Zhang,Guilong Zhang,Xiaojia Zheng,Junchao Qian,Xiaohe Tian,Jiewen Zhou,Jiacai He,Yuanyin Wang,Yiqun Wu,Kai Zhong,Dongqing Cai,Duohong Zou,Zhengyan Wu
Incorporation of T1 and T2 contrast material in one nanosystem performing their respective MR contrast role and simultaneously serving as an efficient drug delivery system (DDS) has a significant potential application for clinical diagnosis and chemotherapy of cancer. However, inappropriate incorporation always encountered many issues, such as low contact area of T1 contrast material with water-proton, inappropriate distance between T2 contrast material and water molecule, and undesirable disturbance of T2 contrast material for T1 imaging. Those issues seriously limited the T1 or T2 contrast effect. In this work, we developed a yolk-like Fe3O4@Gd2O3 nanoplatform functionalized by polyethylene glycol and folic acid (FA), which could efficiently exert their tumor targeted T1–T2 dual-mode MR imaging and drug delivery role. First, this nanoplatform possessed a high longitudinal relaxation rate (r1) (7.91 mM–1 s–1) and a stronger transverse relaxation rate (r2) (386.5 mM–1 s–1) than that of original Fe3O4 (268.1 mM–1 s–1). Second, cisplatin could be efficiently loaded into this nanoplatform (112 mg/g) and showed pH-responsive release behavior. Third, this nanoplatform could be effectively internalized by HeLa cells with time and dosage dependence. Fourth, the FA receptor-mediated nanoplatform displayed excellent T1–T2 dual mode MR contrast enhancement and anticancer activity both in vitro and in vivo. Fifth, no apparent toxicity for vital organs was observed with systemic delivery of the nanoplatform in vivo. Thus, this nanoplatform could be a potential nanotheranostic for tumor targeted T1–T2 dual-mode MR imaging and chemotherapy.
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高影响因子
BIOMACROMOLECULES
·
2021
·
IF 6.988
Dongxu Zhou,Siyu Li,Zhixiong Fei,Peng Zhou,Yaqi Zhao,Lunhao Zhi,Chenxi Li,Xu Peng,Xiaoling Liu,Changsheng Zhao
Smart insulin delivery systems now play essential roles in diabetes treatment, whereas most existing systems suffer from insufficient regulation against blood glucose. Here, a glucose and pH dual-responsive insulin delivery system with multilevel self-regulation of blood glucose was constructed. Photocross-linked dual-responsive polymersomes were prepared by the self-assembly of the diblock copolymer methoxyl poly(ethylene glycol)-b-poly[3-acrylamidophenylboronic acid-co-2-(diethylamino)ethyl methacrylate-co-2-hydroxy-4-(methacryloyloxy)benzophenone] (mPEG-b-P(AAPBA-co-DEAEMA-co-BMA)) synthesized by reversible addition-fragmentation chain transfer polymerization (RAFT), where insulin and glucose oxidase (GOx) were co-encapsulated inside. It is worth noting that the polymersomes with tunable membrane permeability are the first glucose-responsive platform consisting of both PBA and GOx. According to the pH change produced by gluconic acid, the pH-sensitive monomer DEAEMA endowed the polymersome membrane with multilevelly tunable and self-regulative permeability, further controlling the release behavior of insulin. This multilevel tunability was reflected directly in in vitro insulin release tests and was proven by the self-regulation of blood glucose in vivo. Promisingly, the polymersomes have great potential to be applied for the self-regulation of blood glucose in the treatment of diabetes.
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高影响因子
ACS Applied Materials & Interfaces
·
2020
·
IF 8.758
Lihong Su,Tingjun Chen,Tianxiang Xue,Anzhi Sheng,Liangfen Cheng,Juan Zhang
In this work, the boronic acid–aptamer conjugate (BAAC) is elaborately designed and explored as a recognition unit. The admirable properties of the pH-dependent boronic acid ester are integrated with the specific capturing capability of the modified aptamer; thus, BAAC can efficiently and selectively bind with the target by adjusting the pH values. An electrochemical biosensor based on pH-adjusted BAAC has been further developed for the analysis of CNeu5Gc, an important biomarker of different kinds of cancer. The boronic acid moiety in BAAC can react with CNeu5Gc to form a BAAC–CNeu5Gc complex under acidic conditions, followed by the release of CNeu5Gc from the complex and subsequent capture by the aptamer moiety with the adjustment of the pH value to alkalinity. With simplicity, high specificity, and efficiency, the biosensor exhibits a wide linear range from 2.816 to 3603.960 ng/mL with a low detection limit of 1.224 ng/mL and can be applied to analyze CNeu5Gc in animal food samples. Besides, this work can also provide a kind of modified aptamer, i.e., the chemical capturing group-modified aptamer, to give a new viewpoint for the exploration of other functionalized aptamers.
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高影响因子
Chemosensors
·
2023
·Vol. 11
·期 8
·425
·
IF 4.2
Jiani Li,Fanfan Liang,Li Han,Xiaoxuan Yu,Dingbin Liu,Wensheng Cai,Xueguang Shao
Accurate determination of extra-cellular pH (pHe) and intra-cellular pH (pHi) is important to cancer diagnosis and treatment because tumor cells exhibit a lower pHe and a slightly higher pHi than normal cells. In this work, the characteristic absorption of water in the near-infrared (NIR) region was utilized for the determination of pHe and pHi. Dulbecco’s modified eagle medium (DMEM) and bis (2-ethylhexyl) succinate sodium sulfonate reverse micelles (RM) were employed to simulate the extra- and intra-cellular fluids, respectively. Continuous wavelet transform (CWT) was used to enhance the resolution of the spectra. Quantitative models for pHe and pHi were established using partial least squares (PLS) regression, producing relative errors of validation samples in a range of −0.74–2.07% and −1.40–0.83%, respectively. Variable selection was performed, and the correspondence between the selected wavenumbers and water structures was obtained. Therefore, water with different hydrogen bonds may serve as a good probe to sense pH within biological systems.Keywords:near-infrared spectroscopy;water structure;extra-cellular pH;intra-cellular pH;partial least squares regression
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高影响因子
JOURNAL OF MATERIALS SCIENCE
·
2018
·Vol. 53
·期 15
·10653-10665
·
IF 2.993
Chen Hui,Kuang Ying,Liu Rong,Chen Zhongyin,Jiang Bingbing,Sun Zhengguang,Chen Xueqin,Li Cao
Reducing the side effects and improving the drug utilization are important work in anti-cancer drug delivery. In this paper, a novel dual-pH-sensitive drug delivery system was reported. Mesoporous silica nanoparticle (MSN) was applied to load anti-cancer drug doxorubicin hydrochloride (DOX) and was covered by mono-6-deoxy-6-EDA- β -cyclodextrine ( β -CD-NH 2 ) to block the pores through pH-sensitive boronate ester bond. And the carriers were then coated with methoxy poly(ethylene glycol) (mPEG) through another pH-sensitive benzoic imine bond. mPEG leaving studies, in vitro cellular uptake studies and the flow cytometry analysis, proved that carriers was “stealthy” at pH 7.4, but could be “activated” for cytophagy by cancer cells in weakly acidic tumor tissues (pH 6.5) due to the departure of mPEG. β -CD-NH 2 leaving studies, the in vitro drug release studies and the in vitro cytotoxicity studies proved that boronate ester bond linking MSN and β -CD-NH 2 was stable at both pH 7.4 and 6.5, but could be hydrolyzed intracellular to release DOX for cellular apoptosis due to the lower pH (5.0). In summary, the novel dual-pH-sensitive drug delivery system fabricated with a dynamic protection strategy should have great application potential in anti-cancer drug delivery fields.
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高影响因子
LANGMUIR
·
2021
·
IF 3.882
Yimin Zhou,Cong Chang,Zuhao Liu,Qiuling Zhao,Qingni Xu,Chaohua Li,Yuqi Chen,Yueli Zhang,Bo Lu
Hollow mesoporous silica nanoparticles (HMSNs) served as nanocarriers for transporting doxorubicin hydrochloride (DOX) and indocyanine green (ICG) and were incorporated into a pH-sensitive targeted drug delivery system (DDS). Boronate ester bonds were employed to link HMSNs and dopamine-modified hyaluronic acid (DA-HA), which acted as both the “gatekeeper” and targeting agents (HMSNs-B-HA). Well-dispersed HMSNs-B-HA with a diameter of about 170 nm was successfully constructed. The conclusion was drawn from the in vitro drug release experiment that ICG and DOX (ID) co-loaded nanoparticles (ID@HMSNs-B-HA) with high drug loading efficiency could sustain drug release under acidic conditions. More importantly, in vitro cell experiments perfectly showed that ID@HMSNs-B-HA could well inhibit murine mammary carcinoma (4T1) cells via chemotherapy combined with photodynamic therapy and accurately target 4 T1 cells. In summary, all test results sufficiently demonstrated that the prepared ID@HMSNs-B-HA was a promising nano-DDS for cancer photodynamic combined with chemotherapy.
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高影响因子
JOURNAL OF SOILS AND SEDIMENTS
·
2021
·Vol. 21
·期 2
·775-783
·
IF 3.308
Du Huihui,Du Jingwei,Liu Fangyi,Zhang Yanhuan,Guo Hanwen,Wan Dan
Purpose Antibiotics often coexist with heavy metals in contaminated soils, making their behaviors more complicated than their individual presences. Sorption to soil components deeply affects the mobility, bioavailability, and fate of contaminants. However, limited studies have investigated the cosorption characteristics of tetracycline (TC) and heavy metals to typical soil minerals, particularly phyllosilicates. Materials and methods This study investigates the effect of Cd(II) on TC sorption by kaolinite (Kao, 1:1-type) and montmorillonite (Mont, 2:1-type) via isothermal adsorption, coupled with X-ray diffraction (XRD) and Fourier transform infrared spectroscopy (FTIR). Results and discussion Isothermal results show that TC sorption on both kaolinite (Kao, 1:1-type) and montmorillonite (Mont, 2:1-type) is little affected by Cd(II) at pH 4.5 but increases significantly at pH 8.5. The overall effect of Cd(II) on TC sorption depends on the intensities of two positive processes, i.e., (1) the formation of soluble Cd-TC complex increases sorption compared to free TC molecules and (2) the formation of a ternary clay-Cd-TC complex at clay variable edge sites increases sorption, and one negative process, i.e., competition of Cd(II) with cationic and zwitterionic TC species at the permanently charged sites decreases sorption. XRD analyses demonstrate that TC intercalates into the interlayer ( d 001 = 18.7 Å) of Mont as one monolayer parallel molecule both with and without Cd(II) but adsorbs predominately on external surface of Kao. FTIR results suggest a flat-lying horizontal configuration of adsorbed TC molecules on clays, accompanied by a strong complexation between the carbonyl and amino groups of TC molecules and the surfaces. Besides, the presence of Cd(II) more or less facilitates this complexation. Conclusions We propose that Cd(II) can significantly promote TC adsorption on clay minerals at alkaline pH rather than acidic pH. TC and Cd(II) interact intensely and form strong surface complexation on clay surfaces. Hence, coexistence with Cd(II) may lower the mobility and thus the bioavailability of TC in alkaline soils, which provides a new direction for soil remediation by regulating soil pH using alkaline materials. Graphical abstract
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高影响因子
Journal of Materials Chemistry A
·
2017
·Vol. 6
·期 3
·1275-1283
·
IF 8.867
Rui Zhou,Ningfei Shen,Jian Zhao,Yu Su,Hejun Ren
Magnetic glutathione (GSH)-functionalized nanocomposites (Fe3O4@GSH) were synthesized via an oxidative polymerization method and characterized as a heterogeneous Fenton-like catalyst for 2,4-dichlorophenol (2,4-DCP) degradation at neutral pH (pH = 6.5). The performance of the composite was extensively investigated in terms of catalytic activity and factors (H2O2 dosage, catalyst loading, initial solution pH, and 2,4-DCP concentration) influencing 2,4-DCP degradation. Iron leaching, primary reactive oxidizing species, and catalyst reusability were also investigated. The results showed that the removal efficiency can reach up to 99% within 240 min in the presence of Fe3O4@GSH, whereas pure Fe3O4 cannot operate under the same circumstances. Hydroxyl radicals (˙OH) were found as the main reactive oxidizing species responsible for pollutant degradation, and the final removal efficiency still reached 94% after recycling three times. The role of GSH can be ascribed to the acceleration of iron leaching from the surface of Fe3O4 and collective action for H2O2. The Fe3O4@GSH catalyst may provide new insights into the Fenton-like process for enhancing contaminant degradation.
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高影响因子
SENSORS AND ACTUATORS B-CHEMICAL
·
2022
·
IF 9.221
Yumei Zhang,Liyun Ding,Haowen Zhang,Pan Wang,Haijun Li
Acetylcholine (Ach) is an important neurotransmitter and plays a vital role in the diagnosis and treatment of many neurological diseases. A new acetylcholine optical fiber biosensor is reported based on the nitrogen doped fluorescent carbon quantum dots/ acetylcholinesterase (N-CQDs/AchE) compound, where AchE was immobilized on the surface of N-CQDs by the cross-linking method. N-CQDs were synthesized by the hydrothermal method as fluorescent probe and can be responsible for pH value. The optical properties , morphology, particle size distribution and surface groups of N-CQDs were characterized by UV-Vis spectrophotometer, Fluorescence spectrometer, High-resolution transmission electron microscope (TEM) and Fourier transform infrared spectrometer (FTIR), respectively. The relationship between acetylcholine chloride solution and fluorescence intensity of N-CQDs/AchE complex was detected in detailed. Good linear relationship between fluorescence intensity and the concentration of acetylcholine from 20–200 µM and 1–100 nM with the lower detection limits of 16.28 µM and 0.29 nM, respectively. Compared with other sensors, this new optic fiber sensor immobilized (N-CQDs/AchE) complex is more simple and operable.
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高影响因子
COLLOIDS AND SURFACES A-PHYSICOCHEMICAL AND ENGINEERING ASPECTS
·
2015
·
IF 2.752
Sha Jin,Dian Li,Peng Yang,Jia Guo,Jennifer Q. Lu,Changchun Wang
Stimuli responsive drug delivery systems have attracted considerable attention due to their ability to deliver drugs on demand to special tissues and cells (e.g. cancer cells). In this paper, we fabricated a new kind of redox/pH dual stimuli-responsive biodegradable poly(methacrylic acid) (MAA) nanohydrogels crosslinked by N , N -bis(acryloyl)cystamine (BACy) through a facile reflux-precipitation polymerization. Subsequently polyethylene glycol (PEG) was grafted onto the surface of nanohydrogels to improve their long circulation time. This kind of PEGylated nanohydrogels could be degraded into short linear polymer chains in the solution of 10 mM glutathione (GSH) due to the sensitive disulfide bonds in the cross-linked networks. Interestingly, we found that the hydrophilic PEGylated nanohydrogels could be simultaneously loaded with hydrophilic drug doxorubicin (DOX) and hydrophobic drug paclitaxel (PTX) with high drug loading efficiency because of its regional hydrophobic nature in the nanohydrogels. The cumulative release profile showed that either lower pH (5.0) or reducing environment (GSH) or a combination could significantly accelerate the drug release from the drug-loaded nanohydrogels. In addition, the experimental results on living cells indicated that the multi-drug-loaded system had synergistic effect on cancer therapy.
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高影响因子
JOURNAL OF COLLOID AND INTERFACE SCIENCE
·
2024
·
IF 9.4
Kunjie Wang,Xianrong Liu,Yanan Li,Yunsi Wang,Riming Hu,Bingxin Liu,Peng Zhang,Benhua Xu,Yongcheng Li
Ensuring Ruthenium-based (Ru) catalysts with high metal utilization is a potential and challenging strategy for designing and constructing high catalytic activity electrocatalysts for hydrogen evolution reaction (HER). Herein, Ruthenium single atoms (SA) and Ruthenium nanoparticles (NPs) are simultaneously anchored on hierarchically porous carbon via the self-templates method for the first time. Benefiting from the synergetic effect of hierarchically porous carbon and the coexistence of Ru SA and Ru NPs, the Ru/C-800 shows attractive HER catalytic activity in acidic and alkaline solutions, with low overpotentials to drive the current density of 10 mA cm −2 and the smallest Tafel slope. Density functional theory (DFT) results reveal that the coexistence of Ru SA and Ru NPs can positively affect charge distribution and charge transfer kinetics, and reduce the hydrogen adsorption and water dissociation free energy. This work provides a novel self-templating strategy for synthesizing high catalytic activity catalysts in a wide pH range.
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高影响因子
INTERNATIONAL JOURNAL OF BIOLOGICAL MACROMOLECULES
·
2024
·
IF 7.7
Yilin Yu,Ning Kong,Zhaoyang Hou,Libo Men,Pei Yang,Zhengjin Wang
Developing a packaging material with integrated cushioning, intelligent and active functions is highly desired but remains challenging in the food industry. Here we show that a sponge-like porous hydrogel with pH-indicating and antibacterial additives can meet this requirement. We use polyvinyl alcohol and chitosan as the primary polymers to construct a hydrogel with hierarchical structures through a freeze-casting method in combination with salting-out treatment. The synergy of aggregated polymer chains and the sponge-like porous structure makes the hydrogel resilient and efficient in energy absorption. It also enables rapid movement of molecules/particles and fast reaction due to the large specific surface area of the pore structures and the large amount of free water in it, leading to a sensitive pH-indicating function. The hydrogel shows an obvious color variation within a wide pH range in 3 min. The silver nanoparticles are fixed in the dense polymer networks , enabling a lasting release of silver ions . The porous structure makes the silver ion reach the protected item in a short time, achieving an antibacterial effect against S. aureus and E. coli with little cytotoxicity. This work paves the way for fabricating multifunctional hydrogels for diverse advanced packaging systems.
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高影响因子
危险品化学品经营许可证（带存储）