Probiotics and Antimicrobial Proteins
·
2024
·
IF 4.4
Wang Fei,Li Xiang,Wang Qi,Jin Qian,Xu Shujie,Tang Li,Zeng Zihan,Fu Aikun,Zhu Jinhui,Zhang Qiao,Yang Rongchang,Xiao Yingping,Li Weifen
Suanshui is a traditional fermented vegetable food in the southwest of China that is a rich source of probiotics. This study aimed to screen probiotics from Suanshui that have a protective effect against liver injury using in vivo and in vitro experiments. Eleven strains were isolated according to acid-producing capacity and probiotic properties, including antibacterial activity against pathogenic bacteria, tolerance to artificial gastrointestinal fluid and bile salts, heat resistance, antioxidant capacity, hydrophobicity, autoaggregation capacity, and adhesion capacity. Three probiotic strains, LAB36 ( Lacticaseibacillus paracasei , ( L . paracasei )), LAB19 ( Lactiplantibacillus plantarum ( L . plantarum )), and LAB51 ( Limosilactobacillus fermentum ( L . fermentum )), were obtained by the TOPSIS comprehensive evaluation. Furthermore, in an in vitro model of inflammation induced by LPS treatment of AML12, metabolites derived from the intestinal contents of mice treated with the three strains significantly decreased the gene expression of IL-1β , TNF-α , and Caspase 3 in the LAB (19, 36, 51) + LPS group ( P < 0.05) and significantly increased the gene expression of Bcl-2 compared with those in the untreated LPS group ( P < 0.05). In addition, the LAB36 + LPS group also exhibited significantly decreased gene expression of TLR4 and Bax ( P < 0.05). In vivo experiments, LAB36 pretreatment significantly decreased the levels of ALT, AST, and inflammatory factors (IL-1β and IL-6) in the serum, improved the antioxidant capacity, and reduced apoptosis in the livers of the model mice (liver injury induced by D-GalN/LPS). In summary, LAB36 isolated from Suanshui has a protective effect on liver injury.
✓ 已验证
产品关联
DOI
PubMed
高影响因子
International Journal of Environmental Science and Technology
·
2024
·
IF 3
Dou W.,Song Y.,Zhang Y.,Liu T.,Kong L.,Hu X.,Li D.
Removal of chloride (Cl(− I)) and fluoride (F(− I)) ions is a major issue in metallurgical waste acid recycling. This study explored the synchronous and two-step precipitation approaches using bismuth oxide (Bi 2 O 3 ) to remove Cl(− I) and F(− I). Under the mole ratio of Bi 2 O 3 and halide ions at respectively 0.3 and 0.7 for the first and second steps, residual concentrations of Cl(− I) and F(− I) below 100 and 50 mg/L, respectively, were obtained, where the removal proportions of Cl(− I) and F(− I) in the first and second steps were respectively 1:1.1 and 1:3.3. During the treatment, Bi 2 O 3 was dissolved to produce Bi 3+ , which reacted with Cl(− I) first and then F(− I), leading to the precipitation of bismuth oxychloride and bismuth oxyfluoride. After the removal of Cl(− I) and F(− I), residual Bi 3+ was removed to a final concentration of 1.3 mg/L through introduction of hydrogen sulfide (H 2 S) and recovered as bismuth sulfide (Bi 2 S 3 ). In this method, approximately 97.6% of Bi 2 O 3 was regenerated, additionally, Cl(− I) and F(− I) were transferred to a NaOH/NaCl/NaF concentrate with minimal volume. This two-step approach for the removal of Cl(− I) and F(− I) using Bi 2 O 3 exhibited excellent performance in terms of high efficiency, high reagent recovery rate and low secondary pollution risk.
✓ 已验证
产品关联
DOI
高影响因子
Discover Oncology
·
2024
·Vol. 15
·期 1
·1-10
·
IF 2.8
Fan Ruizhi,Xu Teng,Kuang Yuting
Background Colorectal cancer, which originates from the human colon or rectum, is one of the leading causes of death worldwide. Timely diagnosis and interventional therapy can significantly improve the prognostic survival of colorectal cancer patients, making regular screening and early detection essential. Aim To investigate the regulatory function of lncRNA CTBP1-DT (CTBP1-DT) on colorectal cancer cells and to assess its diagnostic significance. Methods A total of 102 patients with colorectal cancer and 92 healthy individuals were selected. The levels of CTBP1-DT and microRNA-30a-5p (miR-30a-5p) in serum and cell samples of the above subjects were compared by RT-qPCR. The effects of CTBP1-DT and miR-30a-5p dysregulation on the biological functions of colorectal cancer cells were analyzed via CCK-8, flow cytometry and Transwell assays. In addition, the ability of CTBP1-DT and miR-30a-5p to early identify colorectal cancer patients was determined through ROC curve. Results Serum CTBP1-DT was elevated in patients with colorectal cancer, which was obviously higher than in healthy controls. The expression of serum miR-30a-5p was downregulated in colorectal cancer. Both CTBP1-DT and miR-30a-5p have the value of distinguishing colorectal cancer, and the combined diagnostic ability is higher. Knockdown of CTBP1-DT directly targeted miR-30a-5p to repress cell activity and metastatic ability, whereas deregulation of miR-30a-5p eliminated the above inhibitory effects. Conclusion Overexpression of CTBP1-DT has a certain application potential in the diagnosis of colorectal cancer and may be a therapeutic target for colorectal cancer.
✓ 已验证
产品关联
DOI
PubMed
高影响因子
Biomass Conversion and Biorefinery
·
2024
·
IF 3.5
Zhang Wenrui,Huang Jintian
The non-biodegradability and microtoxicity of harmful anions in water, especially hexavalent chromium, have caused serious damage to the environment and human health. Therefore, research on efficient and environmentally friendly adsorbents for the removal of Cr(VI) becomes essential. In this paper, we propose for the first time to effectively remove Cr(VI) anion by aminated graphene oxide/cellulose oxide nanocellulose/sodium alginate porous materials (NH 2 -GO/NOC/SA) prepared by cross-linking aminated graphene oxide with biomass materials (oxidized nanocellulose and sodium alginate) via epichlorohydrin. The porous materials were characterized using tools such as Fourier transform infrared spectroscopy (FTIR), X-ray diffraction (XRD), scanning electron microscopy (SEM), electroluminescence (EDS), Brunauer Emmett Teller (BET), and X-ray photoelectron spectroscopy (XPS) analysis. The optimum adsorption performance of the porous material was obtained by varying the initial concentration (from 100 to 500 mg/L), pH (from 1 to 8), and adsorption time (from 15 min to 72 h) of the Cr(VI) solution. The analysis of the adsorption data showed that the adsorption process was more in line with the pseudo-second-order kinetic equation and Langmuir isotherm model, and the equilibrium adsorption amount reached 394.1 mg/g. The adsorption of Cr(VI) on the NH 2 -GO/NOC/SA porous material was achieved by electrostatic interactions, Schiff base chemical bonding energy, ion exchange, protonation, precipitation, and hydrogen bonding. In conclusion, the experimental results show that the biomass adsorbent NH2-GO/NOC/SA can be consistently applied to remove Cr(VI) from wastewater. Graphical
✓ 已验证
产品关联
DOI
高影响因子
Probiotics and Antimicrobial Proteins
·
2025
·
IF 4.4
Zhao Yuting,Xie Weiqi,Duan Jingyi,Li Fei
Limosilactobacillus reuteri DSM 17938 ( L. reuteri DSM 17938) was one of the most widely used probiotics in humans for gastrointestinal disorders, but few studies have investigated its role in drug-induced liver injury (DILI). Here, we evaluated the efficacy of L. reuteri DSM 17938 using a mouse model of DILI induced by triptolide. Pregavage of L. reuteri DSM 17938 for 1 week remarkably lowered hepatic inflammatory cytokines level and oxidative stress, with diminished serum alanine transaminase and aspartate aminotransferase levels. Metabolomics and RT-qPCR analysis confirmed its ability in ameliorating TP-disrupted hepatic fatty acid β oxidation. Genome annotation of L. reuteri showed its ability to modulate energy metabolism. Targeted metabolomics demonstrated that L. reuteri DSM 17938 modified the short fatty acid profiles in cecum, especially enhancing propionate levels. Further experiments found that L. reuteri DSM 17938 can activate AMPK signaling by upregulating gut microbiota–derived propionate level, thus restoring impaired mitochondrial biogenesis and energy supply processes to recover energy homeostasis, which leads to diminished ROS production and oxidative stress injury in hepatocytes. Besides, AMPK inhibitor dorsomorphin abolished all the effects on propionate protecting mitochondria and energy metabolism. This study established probiotic therapy of L. reuteri DSM 17938 as a preventive intervention for DILI in clinical. We also revealed that L. reuteri DSM 17938 can activate AMPK signaling by propionate, facilitating a deeper understanding of the action mechanism of L. reuteri DSM 17938 against acute liver injury and contributing to the development of its postbiotics and wider applications. Graphical Mechanism illustration of Limosilactobacillus reuteri DSM 17938 alleviating TP-induced liver injury by enhancing AMPK signaling pathway via propionate (Created with Biorender. com).
✓ 已验证
产品关联
DOI
PubMed
高影响因子
MOLECULAR MEDICINE
·
2024
·Vol. 30
·期 1
·1-16
·
IF 6
Xie Bin,Chen Qiong,Dai Ziyu,Jiang Chen,Chen Xi
Background Chronic obstructive pulmonary disease (COPD) is a chronic inflammatory lung disease associated with high morbidity and mortality worldwide. Oxidative injury and mitochondrial dysfunction in the airway epithelium are major events in COPD progression.Methods and resultsThe therapeutic effects of Progesterone (P4) were investigated in vivo and in vitro in this study. In vivo, in a cigarette smoke (CS) exposure-induced COPD mouse model, P4 treatment significantly ameliorated CS exposure-induced physiological and pathological characteristics, including inflammatory cell infiltration and oxidative injury, in a dose-dependent manner. The c-MYC/SIRT1/PGC-1α pathway is involved in the protective function of P4 against CS-induced COPD. In vitro, P4 co-treatment significantly ameliorated H2O2-induced oxidative injury and mitochondrial dysfunctions by promoting cell proliferation, increasing mitochondrial membrane potential, decreasing ROS levels and apoptosis, and increasing ATP content. Moreover, P4 co-treatment partially attenuated H2O2-caused inhibition in Nrf1, Tfam, Mfn1, PGR-B, c-MYC, SIRT1, and PGC-1α levels. In BEAS-2B and ASM cells, the c-MYC/SIRT1 axis regulated P4’s protective effects against H2O2-induced oxidative injury and mitochondrial dysfunctions.Conclusion P4 activates the c-MYC/SIRT1 axis, ameliorating CS-induced COPD and protecting both airway epithelial cells and smooth muscle cells against H2O2-induced oxidative damage. PGC-1α and downstream mitochondrial signaling pathways might be involved.
✓ 已验证
产品关联
DOI
PubMed
高影响因子
Scientific Reports
·
2024
·Vol. 14
·期 1
·1-10
·
IF 3.8
Tong Luqing,Ye Ke,Chen Qun,Wang Xiaoxi,Hu Chi,Xu Qingsheng,Zhou Lihui,Zhan Renya,Tong Ying
Gamma knife radiosurgery (GKRS) is recommended as the first-line treatment for brain metastases of lung adenocarcinoma (LUAD) in many guidelines, but its specific mechanism is unclear. We aimed to study the changes in the proteome of brain metastases of LUAD in response to the hyperacute phase of GKRS and further explore the mechanism of differentially expressed proteins (DEPs). Cancer tissues were collected from a clinical trial for neoadjuvant stereotactic radiosurgery before surgical resection of large brain metastases (ChiCTR2000038995). Five brain metastasis tissues of LUAD were collected within 24 h after GKRS. Five brain metastasis tissues without radiotherapy were collected as control samples. Proteomics analysis showed that 163 proteins were upregulated and 25 proteins were downregulated. GO and KEGG enrichment analyses showed that the DEPs were closely related to ribosomes. Fifty-three of 70 ribosomal proteins were significantly overexpressed, while none of them were underexpressed. The risk score constructed from 7 upregulated ribosomal proteins (RPL4, RPS19, RPS16, RPLP0, RPS2, RPS26 and RPS25) was an independent risk factor for the survival time of LUAD patients. Overexpression of ribosomal proteins may represent a desperate response to lethal radiotherapy. We propose that targeted inhibition of these ribosomal proteins may enhance the efficacy of GKRS.
✓ 已验证
产品关联
DOI
PubMed
高影响因子
Journal of Central South University
·
2024
·Vol. 31
·期 3
·747-761
·
IF 3.7
Suo Jun,Jiao Ke-xin,Yi Jian-hong,Fang Dong,Ruzimuradov Olim
L -ascorbic acid (AA, also known as vitamin C) and dopamine (DA) play important roles in human life activities. When their concentrations are abnormal, they will cause diseases. Therefore, it is of great interest to develop an effective strategy to detect AA and DA levels. Here, anodic aluminum oxide (AAO) films were used as templates to fabricate indium-tin (InSn) alloy nanowires (NWs) by vacuum mechanical injection method, and then Pt nanoparticles were coated on the surface of the InSn NWs by means of the “in-situ discharge reduction” method. Subsequently, the composites were exposed in air for heat treatment to synthesize PtO 2 /indium-tin oxide (ITO) NWs. Finally, PtO 2 /ITO NWs were reduced under H 2 atmosphere to obtain Pt/ITO NWs. The results show that the diameter of the InSn NWs is ∼40 nm and Pt nanoparticles with 2–5 nm are uniformly coated on the surface of the ITO NWs. Additionally, the performance of electrochemical detection of AA and DA on the Pt/ITO NWs electrode is tested by the cyclic voltammetry and differential pulse stripping voltammetry. The Pt/ITO NWs electrode has low detection limits in the detection of AA (66.7 µM) and DA (1 µM), which reveals the good electrochemical detection of AA and DA.
✓ 已验证
产品关联
DOI
高影响因子
IONICS
·
2024
·
IF 2.4
Yan Lichen,Chen Wei,Song Zehui,Xiao Li
A novel NiO/MnO 2 pompon-like hybrid structure was synthesized through a facile one-step hydrothermal in situ growth approach in this paper. NiO nanosheets grow on MnO 2 pompon-like microsphere to form typical hybrid structures which significantly increase the abundant active sites of electrode materials. The resulting NiO/MnO 2 electrode material exhibits an impressive specific capacity of 2706.1 F/g at a current density of 1 A/g. Remarkably, NiO/MnO 2 pompon-like hybrid structure shows excellent cycling performance and its capacity retention is maintained at 84.8% and the charge–discharge efficiency at 96% after 15,000 cycles. Even after undergoing 40,000 cycles, the electrode material maintains an outstanding charge–discharge efficiency of 95.2% with capacity retention of 69%, which revealing promising application as long-term electrode materials for supercapacitors.
✓ 已验证
产品关联
DOI
高影响因子
BRAZILIAN JOURNAL OF CHEMICAL ENGINEERING
·
2024
·
IF 1.5
Su Yongyou,Zhu Bowen,Zhang Yingjie,Meng Qi,Dong Peng
With the rapid development of the global economy, energy crisis and environmental protection problems are in urgent need of solution. And the development of energy-saving and new energy vehicles is an inevitable trend to improve the energy structure, alleviate the conflict between oil supply and demand, and improve the living environment of human beings. Layered high nickel material is considered as one of the most promising cathode materials for power batteries because of its high energy density, high platform potential and good rate capability. However, it has problems such as high dependence on cobalt resources and poor cycling stability. In this paper, the preparation of layered high nickel cobalt-free cathode materials is investigated. Because Fe element has the advantages of large reserves and low price. At the same time, Fe makes the layered structure of the positive electrode more stable. In this paper, LiNi 0.8 Mn 0.17 Fe 0.03 O 2 (Li/TM = 1.01, 1.03, 1.05) materials with different Li/TM ratios were prepared by solid-phase method to investigate the effects of different Li/TM ratios on the structural and electrochemical properties of LiNi 0.8 Mn 0.17 Fe 0.03 O 2 materials. The results show that the materials synthesized with different Li/TM ratios have a layered structure. When the Li/TM ratio is 1.03, the crystal structure is the best and the degree of Li/Ni mixing is the least. The electrochemical performance study shows that the synthesized materials have the optimal electrochemical performance at the Li/TM ratio of 1.03. The electrochemical behavior during lithium embedding/delithiation was also investigated by quasi-in situ XRD, dQ/dV and CV tests. This study will provide some reference and guidance for the development and application of next-generation low-cost cobalt-free lithium-ion batteries.
✓ 已验证
产品关联
DOI
高影响因子
Scientific Reports
·
2025
·Vol. 15
·期 1
·1-10
·
IF 3.9
Zhou Haoyan,Zu Mei,Tang Xinxuan,Chang Yuanxi,Li Shiquan,Yang Hongrui,Lv Chenglong,Cheng Haifeng
Molecular sieves were widely used in the chemical industry, which caused a large number of inactivated molecular sieves to be discarded, resulting in a serious waste of resources and environmental pollution. Recently, recycling of discarded molecular sieves (DMS) has become a research hotspot. This study proposes an economical method using DMS to prepare green pigments mimicking foliage spectrum. The synthesized products were subjected to characterization using X-ray diffraction (XRD), Fourier-transform infrared spectroscopy (FTIR), Ultraviolet-visible-diffuse reflectance spectroscopy (UV-Vis-DRS), and scanning electron microscopy (SEM). The analysis revealed that sulfur radicals can be effectively incorporated as chromophores within the β-cages that are formed during the recrystallization process of sodalite, these cages are highly conducive to accommodating the radicals, thereby providing a protective environment that shields them from external influences. By adjusting the mass ratio of Na 2 CO 3 /S, the visible reflectance spectrum of ultramarine pigments can be easily modified. In addition, camouflage coating based on ultramarine green can be used to simulate the spectral reflectance characteristics of green vegetation. The Spectral Angle Mapping (SAM) system was used to evaluate the simulation effect of the camouflage coating. At a threshold below 98.5%, the camouflage coating still be misidentified as leaves, this method can recycle massive solid waste resources and significantly reduce the preparation cost of imitation green vegetation camouflage pigments.
✓ 已验证
产品关联
DOI
PubMed
高影响因子
TRIBOLOGY LETTERS
·
2025
·Vol. 73
·期 4
·1-16
·
IF 3.3
Sheng Dezun,Yu Hongliang,Zhang Xiao,Zhou Xin
Reducing alloy friction to achieve ultra-low friction is a valuable approach to save energy and reduce pollution from oil use, which is a major challenge for researchers. This study introduces a successful method to achieve ultra-low friction in Ti-6Al-4 V using a hydrated lubricant composed of hydroxyethyl cellulose (HEC). And the effects of speed and concentration on lubricating were investigated. It was found that excessive sliding speeds may lead to lubricant detachment and consequent friction increase, indicating that the adsorption ability of HEC needs to be enhanced in future studies. In addition, when the concentration exceeds 5 wt%, wear loss tends to stabilize across tests with different concentrations, while the friction force increases with rising concentrations. Based on these findings, microscopic studies were conducted to investigate the mechanism of friction reduction. Notably, distinct topographic features resembling ‘valleys’ and ‘plateaus’ were identified on the wear scars in a nanoscale scope. The movement of the surfaces induces the hydrated HEC lubricant to flow from the lower valleys to the higher plateaus, suggesting elastohydrodynamic lubrication mechanisms to form robust films. The valleys serve as lubricant reservoirs, while the plateau tops support the lubricant films to prevent contacts between Ti-6Al-4 V and Si 3 N 4 . The schematic illustrations depict the microscopic mechanisms for achieving of ultra-low friction on Ti-6Al-4 V alloy.
✓ 已验证
产品关联
DOI
高影响因子
危险品化学品经营许可证（带存储）