JOURNAL OF BIOTECHNOLOGY
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2026
·
IF 4
Malik Jan,Kashif Hayat,Israr Khan,Aadil Ahmed Memon,Ibrar Khan
The new metabolite 3-hydroxyphenazine 1-carboxylic acid (3-OH-PCA) was obtained by Aspergillus sclerotiorum ASJ82 whole-cell biocatalysis of phenazine 1-carboxylic acid. Despite the biotechnological perspective, the lack of favorable methods for 3-OH-PCA separation and purification from complex biotransformation media has considerably hampered further experimental studies for large-scale industrial applications because of insufficient amounts of purified compounds. In this context, the present work aimed to develop a simple and proficient technique for the separation and purification of 3-OH-PCA from the crude extraction and culture medium of A. sclerotiorum ASJ82. The static adsorption and desorption characteristics of five macroporous resins were evaluated, namely, HP-20, HZ-818, HZ-801, D001, and D155. Remarkably, compared with the other resins, the HZ-818 resins exhibited notable advantages in the adsorption and desorption of 3-OH-PCA. Additionally, the equilibrium adsorption data of the HZ-818 resins were assessed via the Langmuir and Freundlich isotherm models. The analysis revealed that the experimental data exhibited a favorable fit with the Langmuir isotherm model, providing valuable insights into the adsorption behavior of 3-OH-PCA on the HZ-818 resins. Furthermore, a chromatographic column packed with HZ-818 resins was employed, and dynamic adsorption and desorption experiments were conducted. These tests aimed to establish and optimize the operational parameters required to effectively separate and purify 3-OH-PCA. Under the optimized separation and purification conditions, the average adsorption capacity and desorption ratio of 3-OH-PCA were 21.1 ± 1.2 mg/g dry resin and 88 ± 1.2 %, respectively. After treatment with HZ-818 resin, the 3-OH-PCA content considerably increased. These findings revealed that the purity of 3-OH-PCA was 96.5 ± 2.1 %, whereas the recovery rate was 85.32 ± 1.2 %. The results indicate that the HZ-818 resin has significant potential as an adsorbent for effectively separating and purifying 3-OH-PCA from complex media. Overall, this methodology demonstrates great promise for achieving high yields in the separation and purification of 3-OH-PCA and could be utilized in the large-scale production of 3-OH-PCA purification from A. sclerotiorum ASJ82 extracts in industry, presenting an avenue for further investigation and application.
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JOURNAL OF ETHNOPHARMACOLOGY
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2026
·
IF 6.8
Fu-Yi Shi,Chun Li,Xi-Yang Zhang,Li-Lan Ou,Gui-Sheng Wu,Huai-Rong Luo
Ethnopharmacological relevance Corylin is a natural flavonoid isolated from the seeds of Psoralea corylifolia L., a traditional medicinal herb historically used to treat bone-related disorders such as osteoporosis. However, its role in organismal aging and the underlying molecular mechanisms remain largely unexplored. Aim of the study This study aimed to evaluate the anti-aging efficacy of corylin and elucidate the molecular basis of its action. Materials and methods Using Caenorhabditis elegans ( C. elegans ) as a model organism, we systematically assessed the impact of corylin on lifespan and multiple aging-associated phenotypes, including locomotor capacity, muscle integrity, lipofuscin accumulation, and resistance to thermal, pathogenic, and oxidative stress. Neuroprotective potential was evaluated using transgenic models of Parkinson's and Huntington's diseases. To uncover the mechanisms involved, we analyzed the DAF-16/SKN-1 signaling axis and autophagy-related processes using transgenic reporter strains, RNA interference, and RT-qPCR. Results Corylin treatment significantly extended lifespan, improved locomotor performance, and reduced lipofuscin accumulation. Furthermore, it enhanced resistance to environmental stresses and conferred neuroprotection in models of neurodegeneration. Mechanistically, corylin promoted nuclear translocation of DAF-16 and upregulated the expression of DAF-16/SKN-1 target genes. This transcriptional activation was associated with enhanced autophagic flux and improved mitochondrial quality. Crucially, the beneficial effects of corylin were abolished in daf-16 and skn-1 mutants, as well as under autophagy-deficient conditions. Conclusion Corylin exerts multifaceted anti-aging effects in C. elegans by coordinately activating the DAF-16/SKN-1 signaling axis, thereby enhancing autophagic flux and maintaining mitochondrial homeostasis. These findings identify corylin as a promising natural candidate for aging intervention.
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INTERNATIONAL JOURNAL OF BIOLOGICAL MACROMOLECULES
·
2026
·
IF 8.7
Jie Bai,Yongchun Huang,Yuling Zhao,Lirong Zhang,Fuli Zhu,Yixizhuoma,Yuxin Weng,Shoude Zhang
The structural characterization of polysaccharides requires precise determination of their monosaccharide composition, including the differentiation of D- and L-configurations. Current analytical methods can identify monosaccharide types but often fail to distinguish between enantiomeric configurations, posing a significant challenge for accurate polysaccharide structural elucidation. This study introduces a robust, accurate, and reproducible ultra-performance liquid chromatography-electrospray ionization-tandem mass spectrometry (UPLC-ESI-MS/MS) method to address this limitation. By utilizing ( S )-(−)-1-phenylethylamine (S-PEA) as a chiral derivatizing reagent, monosaccharide enantiomers are converted into diastereomers through the introduction of a new chiral center. This approach enables the simultaneous determination of D/L configurations for aldoses, amino sugars, alditols, and uronic acids, achieving excellent chromatographic baseline separation. Method validation demonstrated excellent linearity (R 2 > 0.9900) within the range of 2.5–160 μg/mL. The limits of detection (LOD) and quantification (LOQ) ranged from 0.04 to 0.11 μg/mL and 0.13–0.35 μg/mL, respectively, indicating high sensitivity. Accuracy was confirmed with recovery rates between 97.23% and 100.67%, while intra- and inter-day precision RSDs were below 3.54%. The method was successfully applied to characterize the monosaccharide composition of six plant-derived polysaccharides, demonstrating its efficacy in identifying both structural types and absolute configurations. These findings establish the proposed method as a valuable tool for advancing the comprehensive structural analysis of polysaccharides.
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Journal of Environmental Chemical Engineering
·
2026
·Vol. 14
·期 2
·121323
·
IF 7.2
Zeyu Wang,Hao Xu,Minyu Suo,Yu Mei,Dzmitry Hrynsphan,Savitskaya Tatsiana,Christian Kennes,Jun Chen
Biodiesel production from dairy wastewater yields crude glycerol containing residual lactose, forming co-substrates suitable for microbial fermentation. This work converts these residues into propionic acid (PA) using Cutibacterium acnes ZW-1 and clarifies how a small lactose fraction reshapes metabolism. ZW-1 was cultivated anaerobically on a 90 % glycerol-10 % lactose co-substrate and on a 100 % glycerol control. The co-substrate system reached 4.36 g/L PA after 14 days, 1.20 g/L higher than the control, and doubled the maximum production rate from 0.39 to 0.80 g/L/d. Lactose addition increased biomass (OD 600 from 0.62 to 0.74), lowered the final pH (5.14–4.68) and raised the intracellular NADH/NAD⁺ ratio from 2.9 to 4.0. Activities of pyruvate carboxylase and lactate dehydrogenase increased by 1.8- and 2.1-fold, indicating strengthened anaplerotic and redox-balancing routes. Untargeted metabolomics identified 454 differential metabolites; galactose metabolism and the pentose phosphate pathway were markedly upregulated, while biotin derivatives, pyrimidine nucleotides, pyridine compounds and fatty acyls showed positive correlations with PA yield. Overall, a modest lactose supplement unlocks more efficient glycerol fermentation and provides a mechanistically supported route for upgrading dairy-related byproducts to value-added PA.
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Inorganic Chemistry Frontiers
·
2026
·
IF 6.4
Rui Feng,Wei Wang,Tianbo Li,Feifei Yang,Xiaoyan Hu,Xinlong Yan,Lianming Zhao,Shijian Lu
The selective production of linear aldehydes from long-chain α-olefins using heterogeneous Rh catalysts faces a challenge, as non-selective sites in unconfined environments severely compromise regioselectivity. Herein, we report a strategy to achieve high regioselectivity and activity in 1-hexene hydroformylation by confining atomically dispersed Rh sites within the micropores of a silicalite-1 (S-1) zeolite, followed by the selective poisoning of external Rh sites. A series of Rh@S-1 catalysts with varying loadings were synthesized by an in situ hydrothermal method. Comprehensive characterization (XRD, XPS, STEM) confirmed the successful incorporation of Rh as atomically dispersed species within the MFI framework while preserving the zeolite’s textural properties. In the hydroformylation of 1-hexene, the unpoisoned catalyst exhibited high conversion (98.3%) but poor regioselectivity (n/i = 2.7). The introduction of bulky thiol poisons (DMBT and TPMT), which are size-excluded from the zeolite micropores, selectively passivated the external Rh sites. Consequently, the reaction was confined predominantly to the internal Rh sites, thereby dramatically boosting the n/i ratio to 59.9 and 9.3 for DMBT and TPMT poisoned catalysts, respectively, at a low 1-hexene concentration. The superior regioselectivity achieved with DMBT over TPMT is attributed to its more effective poisoning of pore-mouth Rh sites. However, it also introduced higher diffusion resistance, leading to lower conversion and higher apparent activation energy. Moreover, the catalysts demonstrated excellent recyclability. This work elucidates the decisive roles of confined internal versus unconfined external Rh sites in the atomically dispersed Rh@S-1 catalyst for 1-hexene hydroformylation. This finding provides a key design principle for developing efficient heterogeneous Rh catalysts for long-chain α-olefin hydroformylation.
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JOURNAL OF STRUCTURAL CHEMISTRY
·
2025
·Vol. 66
·期 5
·1061-1069
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IF 1.4
Hu J. R.,Wang J. H.,Liu J. S.
Two novel complexes, ( p -cymene)Ru( E 2 C 2 B 10 H 9 )(Ph 2 C(OH)CCH 2 ), designated as I ( E = S) and II ( E = Se), are successfully synthesized and characterized using NMR, IR, MS, and elemental analysis techniques. X-ray structural analysis is performed on complex I , which crystallizes in monoclinic system, space group P 2 1 / c with a = 11.6648(12) Å, b = 16.9404(17) Å, c = 15.6876(17) Å, β = 101.7490(10), C 27 H 36 B 10 ORuS 2 , M r = 649.85, V = 3035.0(5) Å 3 , D c = 1.422 g/cm 3 , Z = 4, F (000) = 1328, µ(Mo K α ) = 0.677 mm –1 , R = 0.0505 and ω R = 0.0793 for 3206 observed reflections ( I > 2σ( I )). Structural analysis indicates the coordination of C=C bonds, formation of C–S bonds, and B-substitution at B3 within the o -carborane cage, resulting in the formation of a Ru–B bond. Furthermore, the ruthenium atom coordinated to p -cymene complies with the 18-electron rule.
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JOURNAL OF ELECTROANALYTICAL CHEMISTRY
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2011
·
IF 2.733
Xiaofang Shen,Yan Cui,Yuehong Pang,He Qian
1-Hydroxypyrene (1-OHP) is a widely used biomarker for evaluation of polycyclic aromatic hydrocarbons (PAHs) exposure. In this paper, a 1-OHP sensor, combining pre-concentration and in situ electrochemical determination, was fabricated based on electropolymerized poly(3-methylthiophene) (P3MT). Direct electrochemical properties of 1-OHP on the P3MT modified glass carbon electrode (GCE) were investigated. A pair of well-defined reversible cyclic voltammetric peaks were observed at the P3MT/GCE with Δ E p of 30 mV. The surface coverage ( Г ) of 1-OHP was calculated to be 9.26 × 10 −11 mol cm −2 at the P3MT/GCE and the electron transfer coefficient α was estimated to be 0.53. P3MT offers π-conjugated system to adsorb 1-OHP via π–π stacking interaction. Meanwhile, P3MT films displayed good electrocatalytic activity to the oxidation of 1-OHP. The peak currents of 1-OHP obtained by differential pulse voltammetry were linear with the concentrations of 1-OHP in a range of 0.5–20.0 μM ( R = 0.9953) with a detection limit of 165 nM (S/N = 3) and a sensitivity of 12.72 μA μM −1 cm −2 . The sensor also exhibited good reproducibility (with a relative standard deviation of 1.2%) and long-term stability.
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JOURNAL OF ALLOYS AND COMPOUNDS
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2016
·
IF 3.014
Yao Zhang,Leyu Zhan,Xiangyang Zhuang,Yunfeng Zhu,Neng Wan,Xinli Guo,Jian Chen,Zengmei Wang,Liquan Li
LiBH 4 possesses high ionic conductivity (σ) at a temperature over 373 K and wide window voltage over 5 V, but low σ value below 373 K. Li 2 NH owns excellent ionic conductivity at low temperature but performs in a narrow window voltage (only 0.7 V). xLiBH 4 -Li 2 NH composites (x = 1, 2, 4) were therefore synthesized to eliminate their respective drawbacks. By alternating current impedance spectra, we observed that xLiBH 4 -Li 2 NH (x = 1, 2, 4) composites offer σ of 10 −2 Scm −1 at a temperature over 373 K, and lift σ value by 100 times from that of LiBH 4 at a temperature below 373 K. According to the cyclic voltammetry (CV for 10 cycles) results, the window voltage of 3 V can be achieved for LiBH 4 -Li 2 NH (1:1). X-ray diffraction (XRD) and Fourier Transform infrared spectroscopy (FTIR) measurements on xLiBH 4 -Li 2 NH (x = 1, 2, 4) samples inform that original phases were well preserved after ball milling and temperature ramping. Their chemical stabilities were also demonstrated by differential scanning calorimetry (DSC) measurements. The enhanced lithium mobility at low temperature in LiBH 4 -Li 2 NH should be ascribed to the favorable boundaries between both phases according to the investigations by both in-situ nuclear magnetic resonance (NMR) and high-resolution transmission electron microscope (HRTEM). However, at a temperature above 373 K, the enhanced ionic mobility of LiBH 4 -Li 2 NH should be mainly contributed by HT-LiBH 4 phase.
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ELECTROCHIMICA ACTA
·
2013
·
IF 3.777
Bin Li,Mengqing Xu,Benzhen Li,Yanlin Liu,Liang Yang,Weishan Li,Shejun Hu
The physical and chemical properties of the solid electrolyte interphase (SEI) formed by prop-1-ene-1,3-sultone (PES) on graphite anode in propylene carbonate (PC) based electrolyte for lithium ion battery were investigated by charge–discharge test, scanning electron spectroscopy with energy dispersive X-ray spectroscopy (SEM–EDS), Fourier transform infrared spectroscopy (FTIR), and X-ray photoelectron spectroscopy (XPS). It is found that the charge–discharge performance of the cell LiCoO 2 /natural graphite (NG) using PC-based electrolyte containing 3 wt% PES is superior to that containing 6 wt% propane sultone (PS), an SEI formation additive that has the similar molecular structure to PES but is reduced not as easily as PES. The results from SEM–EDS, FTIR and XPS show that the structure of graphite has been protected and some S-containing species are proven to be components of the SEI, suggesting that the preferable reduction of PES plays an important role in the formation of a protective SEI on NG.
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CERAMICS INTERNATIONAL
·
2018
·Vol. 44
·期 13
·15432-15439
·
IF 3.057
Fengping Ruan,Degang Deng,Hua Yu,Ming Wu,Bowen Chen,Shiqing Xu,Ruoshan Lei
We synthesized and investigated the effect of Eu 2+ ions doping in a novel phosphor-silicate Ca 8 Sc 2 (PO 4 ) 6 (SiO 4 ) phosphor. The structure and photoluminescence properties were determined by X-ray powder diffraction Rietveld refinement , diffuse reflection spectra, emission-excitation spectra, decay curves and temperature dependence spectra. The phosphors showed an asymmetric broad-band blue emission (Eu 2+ ) with peak at 470 nm. Furthermore, we presented the Ca 7.96 Sc 2 (PO 4 ) 6- y (SiO 4 ) 1+ y :0.04Eu 2+ phosphors by co-substituting [Eu 2+ -Si 4+ ] for [Ca 2+ -P 5+ ], and different behaviors of luminescence evolution in response to structural variation were verified among the series of phosphors. The results were attributed to the presence of multi Ca 2+ sites, resulting in the mixing of blue and green emissions for Eu 2+ ions. The complex anion substitution of [PO 4 ] 3- by [SiO 4 ] 4- induced an increased crystal field splitting of the Eu 2+ ions, which caused a decrease in emission energy from the 5d excited state to the 4f ground state and a resultant red-shift from 470 nm to 520 nm. All the properties indicated that the Ca 8 Sc 2 (PO 4 ) 6 (SiO 4 ):Eu 2+ phosphors have potential application for color-tunable WLEDs .
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MATERIALS & DESIGN
·
2017
·
IF 4.364
Quanfeng Tang,Haizeng Li,Yanfang Yue,Qinghong Zhang,Hongzhi Wang,Yaogang Li,Pei Chen
The electrolyte plays an important role in an electrochromic device (ECD), and an ideal electrolyte can greatly improve the electrochromic performance of the device. Here, we report the preparation of a 1-Ethyl-3-methylimidazolium tetrafluoroborate ([Emim]BF 4 )-doped Poly(methyl methacrylate) (PMMA)-based gel electrolyte for electrochromic applications. By the doping of the [Emim]BF 4 , the ionic conductivity of the gel electrolyte is improved (2.9 × 10 − 3 S/cm at room temperature and 10 − 2 S/cm at 40 °C). Most importantly, the addition of the [Emim]BF 4 could reduce the anodic reaction potentials in an ECD, which could enable ECD bleaching at smaller applied voltages and avoid the degradation of optical modulation induced by ion-trapping in tungsten oxide (WO 3 ) films. This novel electrolyte provides a promising platform for low anodic reaction potential electrochromic devices.
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CHEMICAL ENGINEERING JOURNAL
·
2026
·
IF 13.2
Xinglin Wen,Ye Zhu,Yong Pan,Yonglu Yang,Xiaoyue Wan,Ahmed Mebarki,Juncheng Jiang
The direct hydrogenation of 2,4-dinitrotoluene (2,4-DNT) to 1-methyl-2,4-cyclohexanediamine (2,4-MCHD) represents a significant yet non-industrialized green process for synthesizing methylcyclohexane diisocyanate (HTDI), known for its excellent properties. This process faces considerable challenges due to the hydrogenation of the benzene ring, the reduction of two nitro groups, and the need to avoid deamination reactions. In this study, an alkali-free heterogeneous catalytic system was developed for the direct hydrogenation of 2,4-DNT to 2,4-MCHD using Ru nanoclusters supported on γ-Al 2 O 3 . Side reactions such as deamination were effectively inhibited, resulting in a 2,4-MCHD selectivity exceeding 95% and a 2,4-DNT conversion greater than 99%. Kinetic studies indicated that the hydrogenation of 2,4-DNT in this system operates as a tandem reaction, with the hydrogenation of the benzene ring serving as the rate-determining step. Structure-activity relationship studies demonstrated that the valence state of Ru nanoclusters, the acidity and basicity of the catalyst, and the synergistic effect between Ru and the support significantly influence catalytic performance. This work represents the first example of the direct hydrogenation of dinitroaromatic compounds to aliphatic diamines in a system that does not require the addition of alkali elements, thereby establishing a solid foundation for the further exploration of such reaction systems.
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