BIOSENSORS & BIOELECTRONICS
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2017
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IF 7.78
Junchun Wang,Jinjin Guo,Junjun Zhang,Wenjuan Zhang,Yuzhong Zhang
C-reactive protein (CRP) is a widely accepted biomarker of cardiovascular disease and inflammation. In this study, a RNA aptamer-based electrochemical sandwich type aptasensor for CRP detection was described using the functionalized silica microspheres as immunoprobes. Silica microspheres (Si MSs), which have good monodispersity and uniform shape, were firstly synthesized. The silica microspheres functionlized with gold nanoparticles (Au NPs) provided large surface area for immobilizing signal molecules (Zinc ions, Zn 2+ ) and antibodies (Ab). RNA aptamers, which were specific recognized to CRP, were assembled on the surface of Au NPs modified electrode via gold-sulfur affinity. In the presence of CRP, a sandwich structure of aptamer-CRP-immunoprobe was formed. Square wave voltammetry (SWV) was employed to record the sensing signal, and a clearly reductive peak corresponding to Zn 2+ at about −1.16 V (vs. SCE) was obtained. Under optimal conditions, the aptasensor showed wide linear range (0.005 ng mL −1 to 125 ng mL −1 ) and low detection limit (0.0017 ng mL −1 at a signal-to-noise ratio of 3). Some possible interfering substance was also investigated, and the results obtained showed that the aptasensor possessed good selectivity . When the aptasensor was applied to real serum samples analysis, the satisfied results were obtained, indicating that the aptasensor possessed potential real application ability.
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高影响因子
ANALYST
·
2020
·Vol. 146
·期 3
·896-903
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IF 3.978
Xiaolei Xie,Yufei Wang,Xiaobin Zhou,Junyang Chen,Mengke Wang,Xingguang Su
Single-atom nanozymes have drawn wide attention in bio-sensing for their remarkable merits such as low cost, high stability, and maximum atom utilization. Herein, a colorimetric strategy based on Fe–N–C single-atom nanozymes (Fe/NC-SAs) was established for the detection of alkaline phosphatase (ALP) activity. The Fe/NC-SAs prepared by pyrolysis have excellent peroxidase-like activity and can oxidize 3,3′,5,5′-tetramethylbenzidine (TMB) to a blue color product in the presence of hydrogen peroxide (H2O2). When ascorbic acid (AA) is added to the system, the blue color fades, and the absorbance has a linear relationship with the concentration of AA. Alkaline phosphatase (ALP) can catalyze the hydrolysis of ascorbic acid 2-phosphate (AAP) to produce AA. Thus, a strategy based on Fe/NC-SAs for the detection of ALP activity was established, which provided a linear range of 0.1–1.5 U L−1 and a limit of detection as low as 0.05 U L−1. Besides, Fe/NC-SAs showed high stability under harsh conditions. Moreover, an Fe/NC-SA-based assay was successfully validated using human serum samples for ALP determination with satisfactory results, and has broad prospects in the field of biosensing.
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DESALINATION
·
2025
·
IF 9.8
Xuemei Zhuo,Chunxi Hai,Yanxia Sun,Shengde Dong,Luxiang Ma,Xin He,Qi Xu,Masayoshi Fuji,Takashi Shirai,Yuan Zhou
Spinel-structured titanium‑lithium adsorbents have emerged as a promising solution for efficiently recovering Li + from Salt Lake brines with high Mg/Li ratio, owing to their stable structure, minimal dissolution loss, and excellent cycling stability. However, their practical application remains limited due to low adsorption capacity and prolonged equilibration times. In this study, we developed a novel 5TiO 2 ·2H 2 O/C adsorbents, synthesized by eluting Li 4 Ti 5 O 12 /C precursors with 0.15 mol/L HCl, which exhibits high adsorption capacity and minimal dissolution loss. Under optimal conditions, the 5TiO 2 ·2H 2 O/C adsorbents reached adsorption equilibrium within 5 h at 60 °C in a 600 ppm LiOH solution, with a maximum adsorption capacity of 41.84 mg/g and a titanium dissolution loss of only 0.29 %. The adsorption selectivity of the 5TiO 2 ·2H 2 O/C adsorbents was found to be strongly pH-dependent. In a 170 ppm Li + solution, the adsorbents demonstrated superior cycling stability in original Salt Lake brines, outperforming those in LiCl and Li 2 SO 4 solutions. This study provides valuable insights into the formation and mechanisms of action of these adsorbents, offering significant implications for the efficient recovery of Li + from various brines.
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Journal of Energy Storage
·
2025
·
IF 9.8
Xiaomin Hu,Zhe Chen,Jiayou Ji,Liang Li,Huabo Huang,Shidi Wei
Fiber-shaped supercapacitors (FSCs) serve as optimal energy storage components in wearable platforms. However, traditional fiber materials suffer from poor interfacial cross-linking, disordered microstructures, insufficient active sites, and low electrical conductivity. To overcome these challenges, this research develops Zn-MoO₃/Ti₃C₂Tₓ hybrid fibers via wet-spinning technology employing glacial acetic acid as a coagulation bath, with MoO₃ quantum dots intercalated into Ti₃C₂Tₓ interlayers and Ti₃C₂Tₓ nanosheets cross-linked by Zn 2+ . The resulting hybrid fibers feature expanded interlayer spacing, a large ion-accessible surface area, abundant porous channels, exceptional electrical conductivity, and excellent mechanical properties, delivering an exceptional volumetric capacitance of 2078.2 F cm −3 at 2 A cm −3 with 75.0 % capacitance retention at 20 A cm −3 . Furthermore, the constructed FSCs achieve a substantial energy density of 55.6 mWh cm −3 at a power density of 400 mW cm −3 , with 94.7 % capacitance retention after 5000 cycles, and successfully power an electronic timer, demonstrating their potential for wearable applications.
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Journal of Oncology
·
2021
·
IF 4.375
Xiaodan Sun,Peiyan Zhao,Hui Li,Yan Liu,Tianming Wang,Ying Cheng
Ginsenoside Rh2 (Rh2) is one of the pharmacologically active components of ginseng with an antitumor effect. However, its effect on non-small-cell lung cancer (NSCLC), especially on aerobic glycolysis, which plays a crucial role in the proliferation and progression of tumor cells, has not been characterized. Here, we demonstrated that Rh2 inhibited the proliferation and metastasis of NSCLC cells by promoting apoptosis and suppressing epithelial-mesenchymal transition, respectively. Notably, Rh2 exerted a glycolysis inhibition effect through regulating GLUT1, PKM2, and LDHA, which are key enzymes of the glycolysis process. Furthermore, the metabolic shift function of Rh2 was dependent on the STAT3/c-Myc axis in NSCLC. This novel regulatory role of Rh2 provides a new perspective for NSCLC treatment and highlights the potentiality of Rh2 to be used as a tumor energy blocker. The combination of Rh2 with an STAT3 or c-Myc inhibitor revealed a promising therapeutic approach for patients with NSCLC.
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高影响因子
Frontiers in Pharmacology
·
2022
·
IF 5.988
Pei Xie,Li-Jun Yan,Hong-Ling Zhou,Hui-Hui Cao,Yuan-Ru Zheng,Zi-Bin Lu,Hua-Yi Yang,Jia-Mei Ma,Yu-Yao Chen,Chuying Huo,Chunyang Tian,Jun-Shan Liu,Lin-Zhong Yu
Background: Acute lung injury (ALI) is a serious inflammatory disease with clinical manifestations of hypoxemia and respiratory failure. Presently, there is no effective treatment of ALI. Although emodin from Rheum palmatum L. exerts anti-ALI properties, the underlying mechanisms have not been fully explored.
Purpose: This study aimed to investigate the therapeutic effect and mechanism of emodin on LPS-induced ALI in mice.
Methods: RAW264.7 cells and zebrafish larvae were stimulated by LPS to establish inflammatory models. The anti-inflammatory effect of emodin was assessed by ELISA, flow cytometric analysis, and survival analysis. In vitro mechanisms were explored by using Western blotting, luciferase assay, electrophoretic mobility shift assay (EMSA), and small interfering RNA (siRNA) approach. The acute lung injury model in mice was established by the intratracheal administration of LPS, and the underlying mechanisms were assessed by detecting changes in histopathological and inflammatory markers and Western blotting in lung tissues.
Results: Emodin inhibited the inflammatory factor production and oxidative stress in RAW264.7 cells, and prolonged the survival of zebrafish larvae after LPS stimulation. Emodin suppressed the expression levels of phosphorylated JNK at Thr183/tyr182 and phosphorylated Nur77 at Ser351 and c-Jun, and increased the expression level of Nur77 in LPS-stimulated RAW264.7 cells, while these regulatory effects of emodin on Nur77/c-Jun were counteracted by JNK activators. The overexpression of JNK dampened the emodin-mediated increase in Nur77 luciferase activity and Nur77 expression. Moreover, the inhibitory effect of emodin on c-Jun can be attenuated by Nur77 siRNA. Furthermore, emodin alleviated LPS-induced ALI in mice through the regulation of the JNK/Nur77/c-Jun pathway.
Conclusions: Emodin protects against LPS-induced ALI through regulation on JNK/Nur77/c-Jun signaling. Our results indicate the potential of emodin in the treatment of ALI.Keywords: acute lung injury, inflammation, lipopolysaccharide, emodin, JNK/Nur77/c-Jun
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高影响因子
PHYTOMEDICINE
·
2022
·
IF 6.656
Mengyang Wang,Wu Luo,Tianxiang Yu,Shiqi Liang,Chunpeng Zou,Jinfeng Sun,Gao Li,Guang Liang
Background Heart failure is a common event in the course of hypertension. Recent studies have highlighted the key role of the non-hemodynamic activity of angiotensin II (Ang II) in hypertension-related cardiac inflammation and remodeling. A naturally occurring compound, diacerein , exhibits anti-inflammatory activities in various systems. Hypothesis/Purpose In this study, we have examined the potential effects of diacerein on Ang II-induced heart failure. Methods C57BL/6 mice were administered Ang II by micro-osmotic pump infusion for 4 weeks to develop hypertensive heart failure. Mice were treated with diacerein by gavage for final 2 weeks. RNA-sequencing analysis was performed to explore the potential mechanism of diacerein. Results We found that diacerein could inhibit inflammation, myocardial fibrosis , and hypertrophy to prevent heart dysfunction, without the alteration of blood pressure. To explore the potential mechanism of diacerein, RNA-sequencing analysis was performed, indicating that MAPKs/c-Myc pathway is involved in that cardioprotective effects of Diacerein. We further confirmed that diacerein inhibits Ang II-activated MAPKs/c-Myc pathway to reduce inflammatory response in mouse hearts and cultured cardiomyocytes . Deficiency of MAPKs or c-Myc in cardiomyocytes abolished the anti-inflammatory effects of diacerein. Conclusion Our results indicate that diacerein protects hearts in Ang II-induced mice through inhibiting MAPKs/c-Myc-mediated inflammatory responses, rendering diacerein a potential therapeutic candidate agent for hypertensive heart failure.
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CHEMICAL ENGINEERING JOURNAL
·
2025
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IF 13.2
Bohan Zhang,Jinglei Yang,Qiaojun Jiang,Ruping Zheng,Liting Qiu,Jingxin Chen,Ruiyun You,Zixing Yan,Yudong Lu
In clinical settings, there is a significant demand for the monitoring of therapeutic drugs. Surface-enhanced Raman spectroscopy (SERS) has shown considerable potential in the field of drug analysis. Designing SERS sensors with high sensitivity, strong stability, excellent reproducibility is a challenging and fascinating goal in the field of chemical measurement. In this study, a simple and portable AuNPs/C-CNF/PVA hydrogel SERS sensor was developed, composed of gold nanoparticles (AuNPs), carboxylated cellulose nanofibers (C-CNF) and polyvinyl alcohol (PVA). Due to the “screening” effect of the hydrogel network, it is capable of separating hydrophobic molecules from macromolecules and enabling the enrichment of hydrophilic small molecules. When used to analyze 6-thioguanine (6-TG) and methylene blue (MB) in plasma, the AuNPs/C-CNF/PVA hydrogel SERS sensor showed limit of detection (LOD) as low as 7.9 × 10 −8 M for 6-TG and 3.3 × 10 −9 M for MB. Based on this technology, in blood spiking experiments using clinical samples, the recovery rate for 6-TG ranged from 99.16 % to 103.51 %, while the recovery rate for MB ranged from 93.13 % to 101.70 %. Therefore, the AuNPs/C-CNF/PVA hydrogel SERS sensor enables ultra-sensitive, integrated detection of drugs in plasma through separation and enrichment, without the need for complex pretreatment steps. This approach holds great promise for application in clinical therapeutic drug monitoring.
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CORROSION SCIENCE
·
2025
·
IF 8.5
Fu-Chen Liu,Bo-Wen Chen,De-Wei Ni,Qian Wang,Fei-Yan Cai,Chun-Jing Liao,Hong-Da Wang,Yu-Sheng Ding,Shao-Ming Dong
In this work, YB 4 -CrSi 2 surface-modified C f /ZrB 2 -SiC composites were fabricated by a slurry coating method. The cyclic ablation resistance of the composites was studied at 2600 °C for 300 s using an air plasma test. The ablation mechanisms of the composites were discussed. The Y 3+ can prevent the phase transition of the ZrO 2 and increase the structural stability of the oxide layer. But the oxide layer cannot prevent the inward O penetration resulting from the forming of Zr 0.9 Y 0.1 O 1.95 grains with abundant oxygen vacancy. After the modification of CrSi 2 , the size of Zr 0.9 Y 0.1 O 1.95 grains can be reduced to nano scale. The adequate SiO 2 glassy phase with the uniform distribution of the Zr 0.9 Y 0.1 O 1.95 nano grains can prevent the O penetration effectively and reduce the mass recession rate of the composites.
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INTERNATIONAL JOURNAL OF HYDROGEN ENERGY
·
2025
·
IF 8.3
Senmiao Zhao,Tianxin Wu,Ke Wang,Qiyue Wang,Xiaodan Mei,Jinglan Huang,Haizhen Liu,Cunke Huang,Jin Guo
In this study, a Ni–C co-dispersed catalyst (denoted as Ni&C) with a uniform spherical morphology (≈2 μm diameter) and Core-shell structure was synthesized using Ni-based metal-organic framework (MOFs) as precursors. The Core-shell Ni&C catalyst significantly improved the dehydrogenation kinetics of MgH 2 . The MgH 2 –Ni&C composite exhibited a remarkably low onset dehydrogenation temperature of 192.6 °C, which is 136.9 °C lower than that of pure MgH 2 . The peak dehydrogenation temperature and dehydrogenation temperature span were reduced by 120 °C and 31.3 °C, respectively. At 300 °C, the composite released 5.82 wt% H 2 within 2.1 min. The apparent activation energy for dehydrogenation decreased to 76.78 kJ mol −1 , far below that of pure MgH 2 (138.18 kJ mol −1 ). Mechanistic analysis revealed that Mg 2 NiH 4 /Mg 2 Ni phases formed in-situ during hydrogen cycling acted as "hydrogen pumps" and nucleation promoters of matrix, while the carbon provided good spatial confinement and enhanced thermal conductivity . Moderate coupling between Ni and C generated synergistic effects , collectively optimizing the dehydrogenation performance.
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Journal of Materials Chemistry C
·
2025
·
IF 5.1
Mengmeng Zhang,Zhuoran Song,Daohu Sheng,Yantao Duan,Qi Zhao,Siyao Chen,Weijin Li,Aming Xie
Two-dimensional (2D) materials are considered excellent electromagnetic wave absorbing (EMW) materials because of their ultrathin characteristics, ultra-large specific surface area, and excellent processing properties. However, existing preparation methods (e.g., mechanical stripping, chemical vapor deposition) are difficult to balance between cost, scale and quality. In this study, polymer semiconductor p(C3O2)x with controllable 2D nanosheets micromorphology and enriched with oxygen-containing groups is introduced as a precursor. Its rich oxygencontaining groups can be used as anchor sites for metal ions. The results show that the prepared samples all exhibit a 2D nanosheets structure. Due to the structural features of 2D materials, numerous heterointerfaces are formed within the composites, greatly promoting interfacial polarization. Moreover, oxygen incorporation and crystalline defects substantially improve dipole polarization. The incorporation of metallic components further boosts the magnetic loss performance. Benefiting from the combined influence of multiple attenuation mechanisms and distinctive microstructural properties, the OC-Cu achieves a minimum reflection loss (RLmin ) of -63.7 dB and an effective absorption bandwidth (EAB) of 6.44 GHz. This work provides a new idea for the construction of high-performance EMW absorbing materials.
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JOURNAL OF ALLOYS AND COMPOUNDS
·
2025
·
IF 6.3
Sisi Lin,Tingrui Li,Jing Yu,Kun Wang,Chao Zhang,Xiangdong Ma,Xiaokang Li,Junxia Meng,Xiurong Zhu,Xianke Zhang,Jujun Yuan
For next-generation microwave absorption materials (MAMs), the key performance benchmarks include: (i) minimized thickness matching parameters, (ii) maximized effective absorption bandwidth, and (iii) optimized reflection loss profiles. One-dimensional (1D) magnetic Ni@C porous nanorods have been successfully fabricated through the calcination of ZnNi-MOF precursors under an argon atmosphere. The Ni@C porous nanorods exhibit exceptional electromagnetic wave (EMW) absorption performance, achieving a minimum reflection loss (RL min ) of −64.4 dB at 1.89 mm thickness and an impressive effective absorption bandwidth (EAB) of 5.7 GHz at 2.50 mm thickness at a low filler loading of only 10 wt%. Furthermore, radar cross-section (RCS) simulations indicate the Ni@C porous nanorods achieve a substantial 33.07 dB·m² CST reduction compared to the pure perfect electric conductor (PEC) plate. The outstanding EMW absorption performance originates from the unique structural design of the porous Ni@C nanorods, which simultaneously optimizes impedance matching and incorporates multiple loss mechanisms including conductive loss, dipolar polarization, magnetic resonance, and interfacial polarization effects. This robust synthetic approach presents a versatile platform for developing various 1D magnetic carbon-based composites with superior electromagnetic wave attenuation properties.
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