FOOD SCIENCE AND BIOTECHNOLOGY
·
2022
·Vol. 32
·期 1
·11-19
·
IF 3.231
Peng Wenping,Wang Nan,Wang Shunmin,Wang Junzhen,Bian Zixiu
The phenolic substances, antioxidant capacity, and enzyme inhibitory activity of germinated Fagopyrum tataricum (Tartary buckwheat) under different microwave and l -phenylalanine ( l -Phe) were investigated for the potential of enriching polyphenols. With the germination of seeds, the contents of total phenolics and total flavonoids increased, the antioxidant capacity and enzyme inhibitory activity were enhanced. The highest contents of total phenolics and total flavonoids in Tartary buckwheat sprouts were 17.41 mg GAE/g and 6.26 g RE/100 g DW (7 days), respectively. Correlation analysis and principal component analysis indicated that T 3 (microwave 250 W, 90 s; l -Phe 2.9 mmol/L) could effectively improve the content of polyphenols, enzyme inhibition activity and antioxidant capacity of Tartary buckwheat sprouts obviously. This study hopes to provide some new ideas for enriching phenolics and improving antioxidation of Tartary buckwheat sprouts.
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高影响因子
MOLECULES
·
2022
·Vol. 27
·期 20
·6857
·
IF 4.927
Min Shi,Guanwei Peng,Shuya Xue,Jingkun Xu,Yansha Gao,Shuwu Liu,Xuemin Duan,Limin Lu
In this work, a two-dimensional leaf-like framework-L embedded electrochemically reduced graphene oxide (ERGO@ZIF-L) was proposed as an outstanding electrode material for the sensitive electrochemical sensing of benomyl (BM). ZIF-L is surrounded by ERGO, which could effectively ensure the stability and dispersion of ZIF-L. With this unique combination, the prepared ERGO@ZIF-L displayed excellent synergistic characteristics with a large surface area, excellent conductivity, plentiful active sites, and high electrocatalytic properties, thus endowing it with high sensitivity for BM determination. The experimental parameters, such as solution pH, material volume, and accumulation time, were optimized. Under optimal conditions, the BM sensor showed a wide linear range (0.009–10.0 μM) and low-limit detection (3.0 nM). Moreover, the sensor displayed excellent stability, repeatability, and reproducibility, and good anti-interference capability. The method was successfully applied to detect BM in real-world samples.Keywords:two-dimensional MOFs;zeolitic imidazolate framework;ERGO;benomyl;electrocatalysts;electrochemical sensor
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Polymers
·
2022
·Vol. 14
·期 12
·2317
·
IF 4.967
Juliene Oliveira Campos de França,Deborah da Silva Valadares,Mateus Freitas Paiva,Sílvia Cláudia Loureiro Dias,José Alves Dias
Poly(lactic acid) (PLA) is an important polymer that is based on renewable biomass resources. Because of environmental issues, more renewable sources for polymers synthesis have been sought for industrial purposes. In this sense, cheaper monomers should be used to facilitate better utilization of less valuable chemicals and therefore granting more sustainable processes. Some points are raised about the need to study the total degradability of any PLA, which may require specific composting conditions (e.g., temperature, type of microorganism, adequate humidity and aerobic environment). Polymerization processes to produce PLA are presented with an emphasis on D,L-lactic acid (or rac-lactide) as the reactant monomer. The syntheses involving homogeneous and heterogeneous catalytic processes to produce poly(D,L-Lactic acid) (PDLLA) are also addressed. Additionally, the production of blends, copolymers, and composites with PDLLA are also presented exemplifying different preparation methods. Some general applications of these materials mostly dedicated to the biomedical area over the last 10–15 years will be pointed out.Keywords:poly(lactic acid)-PLA;polymerization based on D,L-lactic acid;D,L-lactide;stereo polymerization of D,L-lactic acid;blends of PLA;poly(DL-lactide-co-polymer);composites of PDLLA
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RSC Advances
·
2022
·Vol. 12
·期 36
·23437-23446
·
IF 4.036
Lina Wang,Qi Wu,Ru Yu,Hongge Zhang,Fei Nie,Wenyan Zhang
A silver nanoparticle-doped Zn(II) metal–organic framework composite (AgNPs@ZnMOF) was investigated as an electrochemiluminescence (ECL) signal enhancer for potassium persulfate. First, ZnMOF was prepared by a one-step hydrothermal method, and then AgNPs@ZnMOF composite was obtained by depositing AgNPs on the surface and interior of ZnMOF. After the AgNPs@ZnMOF composite was modified on the glass carbon electrode (GCE), the cathode luminescence of potassium persulfate on bare GCE was enhanced by 8 times. A dual amplification mechanism provided by Zn(II) and Ag nanoparticles in the AgNPs@ZnMOF composite has been validated by ECL spectra, fluorescence spectra, and electrochemical methods. The interaction between the sulfhydryl groups in L-cysteine (L-Cys) and AgNPs significantly affects the catalytic luminescence of the AgNPs@ZnMOF composite. Thus, a sensitive ECL method for the determination of L-Cys was developed based on the inhibition effect of L-Cys on the ECL signal within the linear range from 5.0 nM to 1.0 μM and the limit of detection was found to be 2 nM (S/N = 3). The established method has been successfully applied to the determination of L-Cys in human urine.
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Analytical Methods
·
2022
·Vol. 14
·期 34
·3260-3269
·
IF 3.532
Yuxuan Wang,Wenhui Xu,Hai Xu,Qiong Jia
Glycoprotein is involved in a variety of biological activities and has been linked to a number of diseases. Glycopeptide enrichment prior to mass spectrometry (MS) detection is crucial to reduce interference, improve detection efficiency, and analyze proteomics deeply and comprehensively. Here, we prepared a novel magnetic hydrophilic material combining tannic acid (TA) and L-cysteine (L-Cys) through a simple and fast procedure. Owing to the synergistic hydrophilic interaction of TA and L-Cys, the obtained adsorbent material shows excellent enrichment performance toward N-glycopeptides with low detection limit, high selectivity, and good reusability. Besides, the material can also be utilized for the enrichment of N-glycopeptides in human serum and saliva, which shows its application prospect in complex biological samples.
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BIOCHEMICAL ENGINEERING JOURNAL
·
2022
·
IF 4.446
Chenyang Qi,Yipin Zhang,Jing Tu
Nanomaterial-based antimicrobial strategies have emerged as powerful tools to combat microbial resistance, avoiding side effects of misusing antibiotics. In this work, the zinc oxide-based nanohybrid (designated as [email protected] ε-PL) as broad-spectrum antibacterial agents were fabricated in a facile two-step process. To be specific, ZnO was coated with near-infrared (NIR)-photoresponsive polydopamine (PDA), and then coupled with the antimicrobial peptide (AMP) (ε-poly- L -lysine, ε-PL). The as-prepared [email protected] ε-PL can effectively contact and capture bacteria, and then exhibit an excellent photothermal effect, i.e . , the temperature rises rapidly to 53.2 °C (100 μg/ml) under 808 nm NIR light irradiation for 10 min. The synergistic effect of ε-PL, ZnO and photothermal agent PDA contributes to the excellent antibacterial efficiency of [email protected] ε-PL (<100 μg/ml) with 808 nm irradiation, that is, 99.70% and 100.00% against Gram-negative bacteria ( Escherichia coli , E. coli ) and Gram-positive bacteria ( Staphyloccocus aureus , S. aureus ), respectively. Further experiments indicated the [email protected] ε-PL ultimately lead to bacterial death by disrupting cell integrity, leaking of intracellular components (e.g., nucleic acids). Moreover, [email protected] ε-PL can effectively remove 70.84% of S. aureus biofilms. Overall, NIR-responsive [email protected] ε-PL has great potential in the field of infection treatment.
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MOLECULES
·
2022
·Vol. 27
·期 13
·4099
·
IF 4.927
Dianwei Zhang,Furui Zhang,Yonghong Liao,Fenghuan Wang,Huilin Liu
This study designed a “turn-off–on” fluorescence analysis method based on carbon quantum dots (CQDs) to detect metal ions and amino acids in real sample systems. CQDs were derived from green pomelo peel via a one-step hydrothermal process. The co-doped CQDs with N and S atoms imparted excellent optical properties (quantum yield = 17.31%). The prepared CQDs could be used as fluorescent “turn-off” probes to detect Fe3+with a limit of detection of 0.086 µM, a linear detection range of 0.1–160 µM, and recovery of 83.47–106.53% in water samples. The quenched CQD fluorescence could be turned on after adding L-cysteine (L-Cys), which allowed detection of L-Cys with a detection limit of 0.34 µM and linear range of 0.4–85 µM. Recovery of L-Cys in amino acid beverage was 87.08–122.74%. Visual paper-based testing strips and cellulose/CQDs composite hydrogels could be also used to detect Fe3+and L-Cys.Keywords:carbon quantum dots;turn-off–on;pomelo peel;fluorescence probe;Fe3+;L-cysteine
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POLYHEDRON
·
2022
·
IF 2.975
Chunqing Wang,Na Xia,Siming Zhu,Lin Chen,Liang Chen,Zhendong Wang
In this study, α- l -rhamnosidase was immobilized on magnetic Fe 3 O 4 /MIL-101 (Cr) nanoparticles using MIL-101(Cr) as precursor by crosslinking method. The crosslinking agent concentration ratio and crosslinking time were EDC/NHS 1:1 and 2 h, respectively. The immobilized materials were characterized by FT-IR, XRD, SEM and VSM. The saturation magnetization of the prepared magnetic nanoparticles was 46.2 emu/g. The optimum pH and temperature of immobilized enzyme were 6 and 60 °C. The application of immobilized enzyme in the preparation of hesperidin dihydrochalcone glucoside (HMGDC) by hydrolysis of hesperidin dihydrochalcone (HSDDC) was studied. The results showed that immobilized enzyme had better temperature resistance, repeatability and stability than free enzyme. The immobilized enzyme activity was 293.55 U/g, and the recovery was 116.03%. After treatment at 60 °C for 4 days, the enzyme activity remained at 24.6%. The conversion rate of HSDDC reached 93.1%.
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MOLECULES
·
2022
·Vol. 27
·期 8
·2419
·
IF 4.927
Xiao-Ling Wang,Wen-Zheng Zhao,Jia-Ze Fan,Le-Chen Jia,Ya-Nan Lu,Ling-Hui Zeng,Yuan-Yuan Lv,Xiao-Yi Sun
Tumor penetration and the accumulation of nanomedicines are crucial challenges in solid tumor therapy. By taking advantage of the MSC tumor-tropic property, we developed a mesenchymal stem cell (MSC)-based drug delivery system in which paclitaxel (PTX)-encapsulating hyaluronic acid-poly (D,L-lactide-co-glycolide) polymeric micelles (PTX/HA-PLGA micelles) were loaded for glioma therapy. The results indicated that CD44 overexpressed on the surface of both MSCs and tumor cells not only improved PTX/HA-PLGA micelle loading in MSCs, but also promoted the drug transfer between MSCs and adjacent cancer cells. It was hypothesized that CD44-mediated transcytosis played a crucial role and allowed deep glioma penetration depending on sequential intra–intercellular delivery via endocytosis–exocytosis. MSC-micelles were able to infiltrate from normal brain parenchyma towards contralateral tumors and led to the eradication of glioma. The survival of orthotopic glioma-bearing rats was significantly extended. In conclusion, the MSC-based delivery of HA-PLGA micelles is a potential strategy for tumor-targeting drug delivery.Keywords:mesenchymal stem cells;hyaluronic acid;poly (lactide-co-glycolide);orthotopic glioma;tumor penetration
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Food Packaging and Shelf Life
·
2022
·
IF 8.749
Peng Zhu,Ying Wang,Xingqun Zhang
In this study, an ethanol extract of Capparis spinosa L. (CSL), as a powerful natural antibacterial and antioxidant agent, was incorporated into polylactide (PLA) nanofibre membranes to fabricate a novel food packaging material. Different contents of CSL (0, 3 and 6 wt%) were added to the PLA solution, and uniform nanofibres were fabricated by electrospinning. The main components in the ethanol extract of CSL, flavonoid compounds, were detected by ultraviolet spectrophotometry . Scanning electron microscopy assays indicated that the CSL-loaded nanofibre membranes possessed an average nanofibre diameter ranging between 201.5 and 377.7 nm. Fourier transform infrared spectroscopy observations showed that CSL was successfully loaded in the nanofibre membranes. The water contact angle test suggested that the concentration of CSL influences the surface hydrophobicity of the nanofibre membranes. Mechanical property tests showed that the addition of CSL improved the flexibility of the nanofibre membranes. The oxidation resistance test indicated that the addition of CSL improved the oxidation resistance of the composite nanofibre membranes. Moreover, the antibacterial analysis suggested that the CSL-loaded nanofibre membranes could effectively inhibit the growth of Escherichia coli (E. coli) and Staphylococcus aureus (S. aureus) . Overall, CSL-loaded composite nanofibre membranes may have promising applications in the field of food packaging.
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ENVIRONMENTAL POLLUTION
·
2022
·
IF 9.988
Yan Li,Shengnan Zhang,Qiongli Bao,Yutan Chu,Hongyu Sun,Yizong Huang
Cadmium (Cd) is a potentially hazardous element with substantial biological toxicity, adversely affecting plant growth and physiological metabolism. Therefore, it is necessary to explore practical and environment-friendly approaches to reduce toxicity. Jasmonic acid (JA) is an endogenous growth regulator which helps plants defend against biological and abiotic stresses. To determine how JA help relieve Cd toxicity in rice, both laboratory and field experiments were implemented. In the seedling stage, the role of JA in mediating rice Cd tolerance was investigated via a fluorescent probe in vivo localization, Fourier Transform Infrared Spectroscopy (FTIR), and colorimetry . At the mature growth stage of rice, field experiments were implemented to research the effects of JA on the Cd uptake and translocation in rice. In the seedling stage of rice, we found that JA application increased the cell wall compartmentalization of Cd by promoting the Cd combination on chelated-soluble pectin of rice roots and inhibited Cd movement into protoplasts , thereby reducing the Cd content in the roots by 30.5% and in the shoots by 53.3%, respectively. Application of JA reduced H 2 O 2 content and helped relieve Cd-induced peroxidation damage of membrane lipid by increasing the level of catalase (CAT), peroxidase (POD), ascorbate peroxidase (APX), and glutathione (GSH), but had no significant effect on the superoxide dismutase (SOD) activity. Additionally, field experiments showed that foliar spraying of JA inhibited rice Cd transport from the stalk and root to the grain and reduced Cd concentration in grain by 29.7% in the high-Cd fields and 28.0% in the low-Cd fields. These results improve our understanding of how JA contributes to resistance against Cd toxicity in rice plants and reduces the accumulation of Cd in rice kernels.
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FOOD CHEMISTRY
·
2022
·
IF 9.231
Yange Ma,He Mei,Yanyan Li,Peipei Zhou,Guangyun Mao,Huili Wang,Xuedong Wang
l -cysteine ( l -Cys) plays an important role in many physiological processes. The previously reported methodologies for l -Cys detection have many drawbacks, and thus the development of specific/sensitive approaches is crucial for its direct/quick assay in food matrices. Herein, silicon quantum dots (SiQDs) and glutathione-stabilized copper nanoclusters (CuNCs) were successfully synthesized and integrated as a ratiometric fluorescent probe for assay l -Cys assay in milks. The fluorescence of SiQDs was quenched by CuNCs through fluorescence resonance energy-transfer process. Upon addition of l -Cys, the 440-nm fluorescence of SiQDs changed insignificantly, while the 650-nm fluorescence of CuNCs decreased significantly. Ratiometric fluorescence signal was linear in the l -Cys concentration range of 0.25 μM to 2.5 mM with a detection limit of 75 nM and visual color changes from red to blue. The probe can realize rapid and portable detection without the participation of intermediate ions, and has good selectivity and accuracy for l -cysteine in milk samples.
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