SENSORS AND ACTUATORS B-CHEMICAL
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2018
·
IF 5.667
Diandian Deng,Hong Yang,Chang Liu,Kang Zhao,Jianguo Li,Anping Deng
In the last years, surface-enhanced Raman scattering based immunochromatographic assay (SERS-ICA) has been getting great attention and been applied for the detection of different target analytes. In this study, a novel SERS-ICA using Ag MBA @SiO 2 -Ab as an immunoprobe has been developed for rapid, quantitative and ultrasensitive detection of a pharmaceutical diclofenac (DCF) residue in water samples. Ag, [email protected] 2 and Ag MBA @SiO 2 nanoparticles (NPs) were synthesized and characterized. The immunoprobe was prepared by sandwiching the Raman reporter mercaptobenzoic acid (MBA) between the core-shell layers and immobilizing the monoclonal antibody (mAb) against DCF on the surface of the [email protected] 2 NPs. Under optimal conditions, the sensitivity and limit of detection (LOD) of the SERS-ICA for DCF were found to be 9 pg mL −1 and 0.07 pg mL -1 , respectively. The detection of the SERS-ICA for DCF was completed within 15 min. Ag MBA @SiO 2 -Ab was stable within four months without significant loss of SERS intensity. The key to success of the SERS-ICA was the employment of the [email protected] 2 NPs as the SERS substrate, which possessed gorgeous SERS enhancement capability, predominant biocompatibility and excellent stability. This SERS-ICA is potential as an ultrasensitive analytical method in point-of-care testing, clinical diagnosis, food inspecting and environmental monitoring.
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APPLIED SURFACE SCIENCE
·
2018
·
IF 4.439
Renxuan Liu,Tao Sha,Qiuhong Zhou,Bei Nie
By employing copper grid as a surrogate agent, we present a facial, reagent-free and cost-effective approach to imprint the exquisite silver dendroid nanostructures on flexible silk fibroin membrane, which double roles as both optical enhancers and catalytic-active sites over many chemical reactions. Unlike the conventional rigid substrates, this hybrid Ag/protein composite exhibits many previous-inaccessible properties of flexibility, transparency, biocompatibility, molecular permeability and optical manipulation. The galvanic displacement was facilitated by a high pressure uv-lamp illumination (365 nm), shortening the dendroid growth time to be within a few minutes. The resulting substrate was characterized by microscopic image, revealing a decent 3D Ag dendrite architecture and interpenetrated crystal network, which arguably enlarged the entire surface area and keenly regulated the optical/electric property of fibrillated protein film. The underlying principle of copper-initiated silver reduction and growth of crystal structure was discoursed and verified with in-situ spectroscopic interrogation. Moreover, our comprehensive study supports that sub-dividable substrate enables to perform catalytic reaction under a wide range of conditions. This exploration renders a thrust to expand biopolymeric membrane into a wide breadth of applications of bendable devices through chemical modifications, exhaustively and economically.
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MICROCHIMICA ACTA
·
2018
·Vol. 185
·期 11
·1-9
·
IF 5.705
Kang Yan,Wu Ting,Han Xiaozhen,Gu Haixin,Zhang Xin
A micro surface-enhanced Raman scattering (SERS) substrate has been fabricated by electrochemical deposition of dendrite-like gold on carbon fiber needles (Au-CFNs). Scanning electron microscopy and energy dispersive spectroscopy were used to confirm the presence of the gold nanostructure on the CFNs. This substrate has a Raman scattering enhancement factor as high as 3.3 × 10^7 when using rhodamine 6G as the reporter molecule. The high SERS sensitivity is attributed to the massive hotspots on gold bulges that enhance the local surface plasmon resonance. The Au-CFN substrate was reproduced 10 times after electrochemically wiping off the analytes from the needle-like electrode. The substrate has attractive features such as convenient sampling, low sample dosage, and minimal invasion. It was applied, in combination with thin-layer chromatography, for the determination of acetamiprid on vegetables. The result was more accurate because the sample information of both the surface and the bulk can be obtained at the same time after inserting the tip of this needle substrate into the TLC plate. The limit of detection for acetamiprid is 0.05 μg⋅mL -1 and the linear range is 0.1–10 μg⋅mL -1 .
A reusable micro needle-like SERS substrate was fabricated and applied for pesticide residue analysis. The SERS signal of acetamiprid can be acquired on the tip of this micro needle-like Au-CFN substrate. This substrate can be reused for 10 times.
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高影响因子
INDUSTRIAL CROPS AND PRODUCTS
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2025
·
IF 6.2
Kuang Luo,Ao Chen,Yiting Liu,Jie Yang,Ning Cai,Jumei Li
Surface-enhanced Raman spectroscopy (SERS) has been perceived as a valuable tool in ensuring food safety due to its rapid and intuitive nature. In this study, we demonstrate a novel cellulose-ZIF-8-noble metal nanostructure as a SERS substrate for the analysis of pesticide residues in food. Cellulose nanocrystal (CNC) acting as a scaffold, we systematically integrate ZIF-8 and Ag nanoparticles to fabricate tailored necklace-like CNC/ZIF-8/Ag core/shell nanostructures. SERS analysis reveals the CNC/ZIF-8/Ag presents an high enhancement factor of 6.39 × 10 4 , a low limit of detection (LOD) of 10 −9 mol/L, and a relative standard deviation of 10.24 % across 20 substrate batches for 4-mercaptobenzoic acid, underscoring its robust sensitivity and reproducibility. Moreover, its utility extends to the quantification of diquat dibromide (DQ) in standard solutions and green tea leaves, showcasing LODs of 6.61 × 10 −9 mol/L and 6.76 × 10 −8 mol/L, respectively, thereby exhibiting promising capabilities for ultra-sensitive pesticide detection in food. The exceptional SERS property can be ascribed to the enriching properties of the porous ZIF-8 nanostructures. Significantly, when exposed to UV light, the CNC/ZIF-8/Ag nanostructures efficiently degrade DQ within 60 min. Furthermore, their practical reusability under UV light is proved by high SERS performance across five detection cycles. These results emphasize the immense potential of CNC/ZIF-8/Ag in SERS-based trace pesticide detection.
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MICROCHIMICA ACTA
·
2024
·Vol. 191
·期 8
·1-10
·
IF 5.3
Wang Panxue,Liu Ying,Li Xiang,Li Cen,Li Guoliang
A ratiometric SERS aptasensor based on catalytic hairpin self-assembly (CHA) mediated cyclic signal amplification strategy was developed for the rapid and reliable determination of Escherichia coli O157:H7. The recognition probe was synthesized by modifying magnetic beads with blocked aptamers, and the SERS probe was constructed by functionalizing gold nanoparticles (Au NPs) with hairpin structured DNA and 4-mercaptobenzonitrile (4-MBN). The recognition probe captured E. coli O157:H7 specifically and released the blocker DNA, which activated the CHA reaction on the SERS probe and turned on the SERS signal of 6-carboxyl-x-rhodamine (ROX). Meanwhile, 4-MBN was used as an internal reference to calibrate the matrix interference. Thus, sensitive and reliable determination and quantification of E. coli O157:H7 was established using the ratio of the SERS signal intensities of ROX to 4-MBN. This aptasensor enabled detection of 2.44 × 10 2 CFU/mL of E. coli O157:H7 in approximately 3 h without pre-culture and DNA extraction. In addition, good reliability and excellent reproducibility were observed for the determination of E. coli O157:H7 in spiked water and milk samples. This study offered a new solution for the design of rapid, sensitive, and reliable SERS aptasensors. Graphical abstract
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COLLOIDS AND SURFACES A-PHYSICOCHEMICAL AND ENGINEERING ASPECTS
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2024
·
IF 4.9
Yu-fang Fu,Jian Zhu,Xin Li,Guo-jun Weng,Jian-jun Li,Jun-wu Zhao
In this work, we synthesized Au-Ag alloy nano-garlands with multiple petal-like branches and internal cavities in two steps. First, we etched silver nanospheres with HAuCl 4 to obtain Au-Ag alloy nanoshells with different shell layer thicknesses and hollow core sizes. After that, based on different nanoshells, 4-NTP was used as a shape-directing agent, HAuCl 4 and AgNO 3 as precursor raw materials, and AA as a reducing agent for the growth of petals to form Au-Ag bimetallic nano-garlands with multi-branching structure. The regulation of the branching morphology of the nano-garlands could be achieved by changing the amounts of 4-NTP and HAuCl 4 . The SERS activity of the nano-garlands could be further improved by changing the AA concentration, the size of nanoshells, and the Au/Ag ratio of the precursor raw materials. Because the bimetallic nano-garlands have hollow cavities and petal-like branches, there are couplings between the cavities and the branches, between the adjacent branches, and between the metals inside and outside of the cores, which can achieve a good enhancement of SERS. The highest SERS enhancement factor of Au-Ag bimetallic nano-garlands can reach 4.47×10 8 , which can be used as a good candidate for SERS substrate for the trace detection of biomolecules , pesticides, and other substances.
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MICROCHIMICA ACTA
·
2017
·Vol. 184
·期 6
·1711-1719
·
IF 4.58
Fu Xiquan,Chu Yanxin,Zhao Kang,Li Jianguo,Deng Anping
The β-adrenergic agonist brombuterol (BB) is illicitly used as an additive in animal feed to enhance the lean meat-to-fat ratio. The authors describe an ultrasensitive lateral flow immunochromatographic assay (LFIA) based on the use of surface enhanced Raman scattering (SERS) for the determination of brombuterol in swine meat and urine samples. Flower-like gold-silver core-shell bimetallic nanoparticles (referred to as AuNF@Ag) displaying strong SERS enhancement were synthesized, characterized and used as the substrate for the preparation of the LFIA. Polyclonal antibody against brombuterol was immobilized on the surface of the AuNF@Ag particles carrying the Raman reporter 4-mercaptobenzoic acid (MBA). After performing an LFIA, the Raman scattering intensity of MBA on the test line was measured and used for quantitation of brombuterol. Figures of merit of this assay procedure include (a) duration of LFIA process of 15 min; (b) an IC 50 value (e.g. the concentration of brombuterol producing 50% of signal inhibition in standard curve) of 380 pg mL-1; and (c) a limit of detection as low as 0.5 pg mL-1. The LFIA is selective over the molecules salbutamol, ractopamine, phenylethanolamine A, isoproterenol and phenylephrine, but shows a 8.5% cross-reactivity to clenbuterol, probably due to the high structural similarity. Swine meat and urine samples spiked with different amounts of brombuterol were analyzed by this method and gave recoveries between 95.8 and 108.0%, and relative standard deviations between 2.0 and 6.3% (for n = 3).
Schematic presentation of the lateral flow immunochromatographic assay (LFIA) based on surface enhanced Raman scattering (SERS) using flower-like gold-silver core-shell nanoparticles. It is capable of detecting brombuterol in swine meat and urine samples with high sensitivity and specificity.
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Materials Science & Engineering C-Materials for Biological Applications
·
2016
·
IF 3.42
Zhijie Zhang,Yunping Wu,Zhihua Wang,Xueyan Zou,Yanbao Zhao,Lei Sun
Silver nanoparticle-embedded polyvinyl alcohol (PVA) nanofibers were prepared through electrospinning technique, using as antimicrobial agents and surface-enhanced Raman scattering (SERS) substrates. Ag nanoparticles (NPs) were synthesized in liquid phase, followed by evenly dispersing in PVA solution. After electrospinning of the mixed solution at room temperature, the PVA embedded with Ag NPs (Ag/PVA) composite nanofibers were obtained. The morphologies and structures of the as-synthesized Ag nanoparticles and Ag/PVA fibers were characterized by the techniques of transmission electron microscopy (TEM), X-ray diffraction (XRD), ultraviolet-visible absorption spectroscopy (UV–vis), Fourier transform infrared spectroscopy (FTIR), scanning electron microscopy (SEM) and energy-dispersive X-ray spectroscopy (EDX). Ag NPs have an average diameter of 13.8 nm, were found to be uniformly dispersed in PVA nanofibers. The Ag/PVA nanofibers provided robust antibacterial activities against both Gram-positive Staphylococcus aureus ( S. aureus ) and Gram-negative Escherichia coli ( E. coli ) microorganisms. It's also found that Ag/PVA nanofibers make a significant contribution to the high sensitivity of SERS to 4-mercaptophenol (4-MPh) molecules.
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高影响因子
Journal of Materials Chemistry C
·
2017
·Vol. 5
·期 48
·12678-12687
·
IF 5.256
Jing Zhao,Li Long,Guojun Weng,Jianjun Li,Jian Zhu,Jun-Wu Zhao
We synthesized a novel bimetallic core–shell–satellite surface-enhanced Raman scattering (SERS) substrate (AuNS@mSiO2@AgNP) that consists of a gold nanostar (AuNS) core, a mesoporous silica (mSiO2) shell and a layer of silver nanosphere (AgNP) satellites. The core–shell–satellite nanostructure was formed by growing branches in the mSiO2 shell of mSiO2 coated gold nanospheres (AuNP@mSiO2) using AuNPs as the seeds, and the subsequent deposition of AgNPs on the mSiO2 surface. The growth of AuNSs in mSiO2 leads to the formation of a large number of closely spaced branches which provide enormous hotspots; and by varying the length of the branches, the nanogap distance between the AuNS core and the AgNP satellites can be carefully adjusted. Besides, mSiO2 could allow the infiltration of the analyte into the nanogap area that has high SERS enhancement. To demonstrate the feasibility of the SERS substrate, the bimetallic core–shell–satellite nanostructures were used for the label-free detection of a pesticide named thiram. The AuNS@mSiO2@AgNP was shown to have excellent selectivity and SERS activity which is significantly higher than that of AuNS@mSiO2, mSiO2@AgNP and AuNP@mSiO2@AgNP. This method exhibits a good linear response when the thiram concentration ranges from 10−9 M to 10−3 M, and the limit of detection (LOD) is 10−9 M, showing that the bimetallic core–shell–satellite nanostructure is a promising SERS substrate.
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TALANTA
·
2024
·
IF 5.6
Lei-Xin Jin,Qi-Meng Wang,Shu-Jiao Zhu,Cai-Lian Zhu,Jian-Jun Sun,Shao-Hua Wu
The flap endonuclease 1 (FEN1) plays a key role in DNA replication and repair, its aberrant expression is associated with tumor development, so it has been recognized as a promising biomarker for a variety of cancers. Here, a novel “turn on” mode gold nanocube-enhanced surface-enhanced Raman scattering (SERS) biosensor was constructed by combining a heated Au electrode (HAuE), exonuclease III (Exo III)-assisted cycle amplification, and gold nanocube (AuNC)-based SERS enhancement to achieve highly sensitive detection of FEN1 activity. The SERS tag was prepared using the Raman reporter modified on the AuNC surface, and the high electromagnetic field provided by the sharp geometric feature of AuNC greatly enhanced the SERS signal. At the same time, HAuE was used to increase the electrode surface temperature and enhance the FEN1 activity, leading to more trigger DNA being cleaved, which was used to initiate the Exo III-assisted cycle amplification. Taking all these advantages, the proposed method possessed high sensitivity and good selectivity, with a low limit of detection (LOD) of 3.19 × 10 −7 U μL −1 . In addition, this method was successfully applied to detect FEN1 activity in real cellular extracts.
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Food Chemistry-X
·
2024
·
IF 6.5
Jinxin Chen,Cheng Zhang,Lunzhao Yi,Fengmin Duan,Ying Gu,Kun Ge,Xuejing Fan
The improvement of detection accuracy without loss of rapidity and sensitivity by optical sensors in complex food analysis is still full of challenges owing to the matrix interference. Herein, a novel and simple self-calibration colorimetric/fluorescence/surface-enhanced Raman spectroscopy (SERS) tri-modal optical sensor based on aminated Rhodamine 6G (R6G-NH 2 ) was developed for highly rapid, sensitive, and accurate detection of Hg 2+ in food samples. The high recognition specificity of R6G-NH 2 for Hg 2+ can be achieved through the metal chelation interaction between Hg 2+ and -NH 2 , -COOH groups in R6G-NH 2 with formation of R6G-NH 2 -Hg 2+ -R6G-NH 2 complex. The DFT and FDTD simulations were adopted to confirm the theoretical feasibility in Hg 2+ detection by tri-modal optical. Under the optimum conditions, the analytical method based on self-calibration tri-modal optical sensor for Hg 2+ detection was established with promising properties (rapidity, linearity, linear range, LOD, and LOQ), providing a strategy in rapid, selective, sensitive, and accurate detection for food safety.
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SPECTROCHIMICA ACTA PART A-MOLECULAR AND BIOMOLECULAR SPECTROSCOPY
·
2024
·
IF 4.3
Xiaoyu Yang,Shunbi Xie,Runzi Zhang,Yao Liu,Weifen Wu,Yi He
Excessive plastic consumption can pose potential risks to the human respiratory and circulatory systems, leading to various diseases. Therefore, the sensitive detection of plastics holds significant implications for ensuring food safety, environmental protection, and human health. Conducting tests on rivers and drinking water can ensure their compliance with relevant safety standards, thereby mitigating the potential environmental and health risks associated with plastic pollution. In this experiment, we prepared a roseate petal homochiral nanogold (Au RHNs) as a surface-enhanced Raman scattering (SERS) substrate for detecting plastics in the water. Due to the intricate rose petal-like surface and structures with symmetry breaking, which result in a large surface area, the mean enhancement factor (EF) of the Au RHNs was determined to be 8.4696 × 10 5 . The Au RHNs as the SERS substrate were used to test the plastic polyethylene (PE) and polyvinyl chloride (PVC), with the detection limits of 0.0986 mg/mL and 0.0975 mg/mL, respectively. Moreover, the prepared Au RHNs substrate were successfully applied for ananlyzing analyze actual samples (tap water, mineral water, river water), yielding a satisfactory recovery rate. The exceptional performance of Au RHNs as a SERS detection substrate indicated its promising potential for practical detection of plastic samples.
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高影响因子
危险品化学品经营许可证（带存储）