Food and Bioprocess Technology
·
2026
·Vol. 19
·期 10
·519
·
IF 5.6
Pei Yiqiao,Yang Xu,Zhang Cheng,Sun Junna,Zhang Xiaohan,Cheng Jayden,Wang Hao,Fan Kaijie
High relative humidity (RH) in prawn storage environments accelerates microbial proliferation, resulting in a shelf life within 3 days at 4 °C. Based on this preservation environment, this study developed an antimicrobial smart film composed of cinnamon essential oil (CEO)-liposome-mussel adhesion proteins (MAP)-polyvinyl alcohol (PVA), evaluated prawn preservation effects, and explored the RH trigger mechanism. The results showed that the film containing CEO-loaded liposomes and 75 mg of MAP (F-CLP 75 ) could reduce the premature loss of CEO under relatively dry conditions. Under high-RH conditions, the film swelled and promoted CEO release while maintaining structural integrity, thereby delaying prawn quality deterioration associated with free water accumulation, microbial proliferation, and protein degradation, and extending the acceptable storage period of prawns to approximately 5–6 days at 4 °C. Additionally, compared with the blank liposome film (F-LP 0 ), F-CLP 75 exhibited significantly improved water resistance, thermal stability, mechanical properties, and barrier properties. This study provides useful guidance for the development of RH-responsive antimicrobial packaging films and the upgrading refrigerated preservation technologies for aquatic products. Graphical
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CELL DEATH AND DIFFERENTIATION
·
2023
·Vol. 30
·期 5
·1260-1278
·
IF 12.4
Sun Ge,Wei Yuntao,Zhou Baosheng,Wang Manlin,Luan Ruina,Bai Yu,Li Hao,Wang Shan,Zheng Dantong,Wang Chunyu,Wang Shengli,Zeng Kai,Liu Shuchang,Lin Lin,He Mingcong,Zhang Qiang,Zhao Yue
The estrogen receptor alpha (ERα) signaling pathway is a crucial target for ERα-positive breast cancer therapeutic strategies. Co-regulators and other transcription factors cooperate for effective ERα-related enhancer activation. Recent studies demonstrate that the transcription factor CTCF is essential to participate in ERα/E2-induced enhancer transactivation. However, the mechanism of how CTCF is achieved remains unknown. Here, we provided evidence that BAP18 is required for CTCF recruitment on ERα-enriched enhancers, facilitating CTCF-mediated chromatin accessibility to promote enhancer RNAs transcription. Consistently, GRO-seq demonstrates that the enhancer activity is positively correlated with BAP18 enrichment. Furthermore, BAP18 interacts with SMARCA1/BPTF to accelerate the recruitment of CTCF to ERα-related enhancers. Interestingly, BAP18 is involved in chromatin accessibility within enhancer regions, thereby increasing enhancer transactivation and enhancer-promoter looping. BAP18 depletion increases the sensitivity of anti-estrogen and anti-enhancer treatment in MCF7 cells. Collectively, our study indicates that BAP18 coordinates with CTCF to enlarge the transactivation of ERα-related enhancers, providing a better understanding of BAP18/CTCF coupling chromatin remodeling and E-P looping in the regulation of enhancer transcription.
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MICROCHIMICA ACTA
·
2022
·Vol. 189
·期 10
·1-11
·
IF 6.408
Zhang Wangbin,Zhao Qianyu,Zhou Xiaoqin,Chen Jie,Liu Yong,Tang Liping,Min Jianxin,Yan Dongmei,Xu Chen,Li Bin,Yang Xin
Magnetic molecular imprinted polymers (MIPs) based on 4-vinylbenzyltrimethylammonium chloride (VBTAC) and 4-vinylbenzoic acid (VBA) deep eutectic solvent as dual functional monomers was successfully synthesized for the specific recognition of laminarin. The MIPs were characterized by transmission electron microscopy, scanning electron microscopy, Fourier transform infrared spectroscopy, thermal gravimetric analysis, and vibrating sample magnetometer analysis. The results showed that the MIPs were spheres of a uniform size, with the surface rich in cavities and excellent superparamagnetism properties. The adsorption experiments showed that MIPs conform to pseudo-second-order kinetics and Langmuir isotherm adsorption. The maximum adsorption capacity under optimal conditions was 322.58 μg·mg −1 and the imprinting factor was 2.13. Under the optimized conditions, the limit of detection (LOD) of the developed material was 6.6 µM. Linearity of the material was obtained within the range 20–800 µM with a coefficient of determination ( r 2 ) being better 0.999. Relative standard deviations (RSDs) were less than 3.96%, and satisfactory recoveries were between 94.55 and 97.39%. The actual sample analysis manifested that MIPs could effectively separate laminarin from Laminarin japonica Aiesch. Graphical abstract
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高影响因子
Chinese Medicine
·
2019
·Vol. 14
·期 1
·1-11
·
IF 2.265
Guo Shangxin,Lin Xiaotong,Wang Yi,Gong Xingchu
Background The discovery of bioactive compounds in traditional Chinese medicine (TCM) has become an important field in TCM modernization. Ligand fishing is a suitable method for discovery of bioactive compounds in complex mixtures such as TCM with high selectivity. Because of unique advantage of low cost and convenience, paper-based microdevices can be good carriers for enzyme immobilized ligand fishing. Methods As an important enzyme for glucose metabolism, α-glucosidase was immobilized on polycaprolactone–chitosan-modified paper to prepare the microdevice with unique microfluid structure generated by 3D printing technology, which can be easily applied to screen active compounds in herbal extracts. The preparation conditions of the paper microarray were optimized. The activity of immobilized α-glucosidase was verified by colorimetric reactions which can be easily monitored by cellphone. The paper microarray with α-glucosidase immobilized was used to screen active compounds in the water extracts of mulberry leaves and lotus leaves. Results Several key parameters including Na 2 CO 3 solution concentration, Na 2 CO 3 solution volume, glutaraldehyde concentration, crosslinking time of glutaraldehyde and time of α-glucosidase immobilization were optimized. The proposed paper-based microarray was successfully applied in screening active compounds in two herbal extracts. Four compounds including chlorogenic acid, quercetin-3- O -glucuronide, isoquercetin, and quercetin were identified as α-glucosidase inhibitors. The compounds with significant non-specific adsorption caused by chitosan, such as isoquercitrin, astragalin, quercetin, were also found to be active compounds. Conclusions An enzyme immobilized paper microarray was designed and fabricated in this work. Polycaprolactone and chitosan were used to modify filter paper to prepare paper microarrays. Parameters of paper device preparation were optimized. Our findings suggested that 3D-printing paper-based microarrays can be a simple and low-cost approach for discovery of active compounds of TCM.
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高影响因子
MICROCHIMICA ACTA
·
2022
·Vol. 189
·期 12
·1-11
·
IF 6.408
Wang Yue,Zhai Hongguo,Yin Jiaqi,Guo Qi,Zhang Yuhao,Yang Qingqing,Li Falan,Sun Xia,Guo Yemin,Zhang Yanyan
With the development of exonuclease, the exonuclease has been used to construct a variety of aptasensor and to realize the signal amplification. Among them, based on silver nanoparticles (Ag NPs) and exonuclease I (Exo I)-assisted cycle signal amplification strategy, we designed a novel high-sensitivity dual-target electrochemical biosensor to detect Pb 2+ or Hg 2+ in water. In the presence of Hg 2+ , the Hg 2+ was fixed to the aptamer chain by thymine-Hg 2+ -thymine (T-Hg 2+ -T), resulting in the decrease of signal. When Pb 2+ was present, DNA single strand S2 dissociated and was bound to Pb 2+ , which automatically triggered Exo I to selectively cut the single chain from the recognition site to achieve the cyclic amplification of the electrochemical signal. The interaction between aptamer and Exo I was investigated by gel electrophoresis. Under the optimum conditions in the scan range -0.20 to 0.60 V, the biosensor had high sensitivity with a linear range of 100 pg/L to 10.0 mg/L, Pb 2+ or Hg 2+ , and the detection limits were 17.0 pg/L ( R 2 = 0.993) and 12.0 pg/L ( R 2 = 0.993), respectively. The relative standard deviation (RSD) of the sensor was 0.5–2.6%, and the recovery of spiked standard solutions was between 98.3 and 110%. The cycle amplification strategy supported by this enzyme has promising applications in detection of the two metal ions in various fields. Graphical Abstract
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MICROCHIMICA ACTA
·
2021
·Vol. 188
·期 10
·1-10
·
IF 5.833
Li Min,Cai Yi-Na,Peng Chi-Fang,Wei Xin-Lin,Wang Zhou-Ping
Copper nanomaterials based on DNA scaffold (DNA-Cu NMs) are becoming a novel fluorescent material, but it is still challenging to obtain highly fluorescent DNA-Cu NMs with excellent stability. In this work, we report a kind of copper nano-assemblies (Cu NASs) with aggregation-induced emission enhancement (AIEE) property using DNA dendrimers with sticky end as template. The sticky end of the DNA dendrimers induced the formation of much bigger Cu NASs with average size ranging from 131 to 264 nm, depending on the length of the DNA dendrimer sticky end from 6 bases to 27 bases. Compared with complete complementary DNA dendrimer, nearly 6-fold fluorescence enhancement was achieved using DNA dendrimer with 27 bases sticky end. Moreover, the DNA dendrimer-Cu NASs demonstrated excellent stability in serum and could be rapidly quenched by Pb 2+ ions. Based on the above property, highly sensitive and selective fluorescent detection of Pb 2+ ions was possible with a linear range of 2.0–100 nM and a detection limit of 0.75 nM. Due to the sensitive and rapid response to Pb 2+ as well as excellent stability in complex matrix, the proposed fluorescent Cu NASs demonstrated high potential as an excellent fluorescent probe for Pb 2+ in complex matrix. Graphical abstract
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ANALYTICAL AND BIOANALYTICAL CHEMISTRY
·
2019
·Vol. 411
·期 17
·3801-3810
·
IF 3.286
Huang Yuanlin,Liang Guangzhao,Lin Tianran,Hou Li,Ye Fanggui,Zhao Shulin
The convenience of colorimetric sensors is useful for practical applications. In this work, we constructed a novel colorimetric sensor with magnetic separation ability that can be operated in nearly neutral conditions and achieve one-step detection of metabolites. Magnetic Cu doped Fe 3 O 4 @FeOOH magnetic nanocomposite (Cu/Fe 3 O 4 @FeOOH) with an oxygen vacancy was prepared by a one-step self-assembly hydrothermal method, and fully characterized by different methods. The oxygen vacancy generated by the incorporation of Cu 2+ cations into the Fe 3 O 4 @FeOOH structure was confirmed to be a vital reactive site for enhancing the catalytic activity, which opens up a new way of designing highly efficient enzyme mimics. Benefiting from its inherent horseradish-peroxidase-like activity, a simple and selective enzyme-based colorimetric sensor was developed for one-step detection of H 2 O 2 and cholesterol, and 3,3′,5,5′-tetramethylbenzidine was catalyzed by H 2 O 2 to generate a colored product of oxidized 3,3′,5,5′-tetramethylbenzidine for signaling. H 2 O 2 and cholesterol can be linearly detected in the same range from 0.01 to 0.4 mmol L -1 with detection limits of 0.0075 mmol L -1 and 0.0082 mmol L -1 , respectively. The proposed colorimetric sensor has satisfactory reusability, accuracy, and practicability in human serum samples, indicating its potential application for the detection of different metabolites in the fields of life science and analytical science.
Graphical abstract
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高影响因子
Nature Communications
·
2021
·Vol. 12
·期 1
·1-14
·
IF 14.919
Wang Yuqin,Guan Xiaoyu,Zhang Shanyu,Liu Yao,Wang Sha,Fan Pingping,Du Xiaoyu,Yan Shuanghong,Zhang Panke,Chen Hong-Yuan,Li Wenfei,Zhang Daoqiang,Huang Shuo
Folding of RNA can produce elaborate tertiary structures, corresponding to their diverse roles in the regulation of biological activities. Direct observation of RNA structures at high resolution in their native form however remains a challenge. The large vestibule and the narrow constriction of a Mycobacterium smegmatis porin A (MspA) suggests a sensing mode called nanopore trapping/translocation, which clearly distinguishes between microRNA, small interfering RNA (siRNA), transfer RNA (tRNA) and 5 S ribosomal RNA (rRNA). To further profit from the acquired event characteristics, a custom machine learning algorithm is developed. Events from measurements with a mixture of RNA analytes can be automatically classified, reporting a general accuracy of ~93.4%. tRNAs, which possess a unique tertiary structure, report a highly distinguishable sensing feature, different from all other RNA types tested in this study. With this strategy, tRNAs from different sources are measured and a high structural conservation across different species is observed in single molecule.
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高影响因子
Food Analytical Methods
·
2020
·Vol. 13
·期 6
·1255-1261
·
IF 2.667
Wu Zhengzong,He Deyun,Cui Bo,Jin Zhengyu,Xu Enbo
Reliable and sensitive detection of Staphylococcal enterotoxin B (SEB) is important to prevent potential human health risk from foods contaminated by SEB. Here, a triple-channel assay was developed for ultrasensitive detection of SEB. Based on the optical characteristics of tetramers composed of gold nanoparticles (AuNPs) and upconversion nanoparticles (UCNPs), the SEB amounts in food products can be simultaneously detected by using surface-enhanced Raman scattering (SERS), fluorometry, and circular dichroism (CD). Under SERS mode, fluorescence mode, and CD mode, wide linear detection ranges (1–750 pg/mL, 1–750 pg/mL, and 2–500 pg·mL −1 ) were achieved. Furthermore, the practicality of the novel assay was evaluated by determining SEB contents in spiked milk samples. These results indicate that the triple-mode aptasensor is a potential useful tool in quantitative detection of SEB in foods.
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高影响因子
JOURNAL OF SOLID STATE ELECTROCHEMISTRY
·
2014
·Vol. 18
·期 10
·2829-2835
·
IF 2.234
Wang Mo,Yin Huanshun,Fu Zhengliang,Guo Yunlong,Wang Xinxu,Zhou Yunlei,Ai Shiyun
MicroRNAs (miRNAs), a class of small endogenous nonprotein-coding RNAs, regulate a wide range of biological processes, and their abnormal expressions are related to the growth and development of plants. Thus, a simple, rapid, and highly sensitive assay for miRNA detection is of great significance. In this work, a label-free and ultrasensitive assay for miRNA detection using protein cage nanoparticles has been developed. Apoferritin-encapsulated Cu nanoparticles (Cu-apoferritin) could be immobilized on the electrode through special reaction between amino and carboxyl. Hybridization event between the probe DNA and the target miRNA-159a is confirmed by electrochemical oxidation signal after Cu released into the detection buffer by adjusting the pH. This assay is highly selective and sensitive with a low detection limit of 3.5 fM. Moreover, the developed method can even discriminate single-base mismatched strand between the complementary targets. The effect of abscisic acid on the expression level of miRNA-159a in Arabidopsis thaliana seeds was also investigated.
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MICROCHIMICA ACTA
·
2019
·Vol. 186
·期 8
·1-9
·
IF 5.479
Liu Hongying,Zhu Langlang,Ma Huan,Wen Jiajun,Xu Hanxiao,Qiu Yubing,Zhang Linan,Li Lihua,Gu Chunchuan
The authors describe the preparation of Cu(II)-coated Fe 3 O 4 ) nanoparticles (NPs) that possess excellent peroxidase-like activity. The NPs were formed by chelation between Cu(II) ions and the oxygen functional groups of sodium ligninsulfonate. The morphology and structure of the NPs were characterized by scanning electron microscopy, transmission electron microscopy, X-ray powder diffraction, X-ray photoelectron spectroscopy, and Fourier transform infrared spectroscopy. The NPs have an average diameter of 220 nm. They are shown to be viable peroxidase mimics that can catalyze the oxidation of 3,3′,5,5′-tetramethylbenzidine by hydrogen peroxide to produce a blue coloration. The findings were used to design a colorimetric assay that has a linear response in the 2.5 to 100 μM H 2 O 2 concentration range and a 0.2 μM detection limit. The assay excels by its selectivity, high sensitivity, good selectivity, portability and cost efficiency.
Fe 3 O 4 -Cu 2+ nanoparticles with excellent peroxidase-like activity were successfully prepared via a facile strategy, and then used to design a facile as well as sensitive colorimetric H 2 O 2 sensor. The linear range and the detection limit were 2.5~100 μM and 0.212 μM.
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高影响因子
ANALYTICAL AND BIOANALYTICAL CHEMISTRY
·
2020
·Vol. 412
·期 4
·915-922
·
IF 3.637
Wu Zhi-Wei,Xie Xing-Chen,Guo Hong-Ran,Xia Huan,Huang Ke-Jing
A tetrahedral DNA probe can effectively overcome the steric effects of a single-stranded probe to obtain well-controlled density and minimize nonspecific adsorption. Herein, a highly sensitive electrochemical biosensor is fabricated for determination of protein using a tetrahedral DNA probe and rolling circle amplification (RCA). N- and P-co-doped graphene (NP-rGO) is prepared, and AuNPs are then electrodeposited on it for DNA probe immobilization. Benefitting from the synergistic effects of the excellent electrical conductivity of NP-rGO, the stability of the tetrahedral DNA probe and the signal amplification of RCA, the biosensor achieves a low limit of 3.53 × 10 −14 M for thrombin and a wide linear range from 1 × 10 −13 to 1 × 10 −7 M. This study provides a sensitive and effective method for the detection of protein in peripheral biofluids, and paves the way for future clinical diagnostics and treatment of disease.
Graphical abstract
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高影响因子
危险品化学品经营许可证（带存储）