MICROCHIMICA ACTA
·
2019
·Vol. 186
·期 8
·1-10
·
IF 5.479
Zhou Yunlei,Jiang Wenjing,Wu Hanwen,Liu Fei,Yin Huanshun,Lu Nan,Ai Shiyun
A nanocomposite was prepared from β-cyclodextrin (β-CD) functionalized graphene oxide and magnetic nanoparticles (GO/Fe 3 O 4 /β-CD). In parallel, a polyamidoamine dendrimer conjugated to avidinylated alkaline phosphatase (PAMAM-avidin-ALP) was prepared and exploited as a signal amplification unit in a voltammetric immunoassay for 5-methylcytosine (5mC) in genomic DNA. The GO/Fe3O4/β-CD as a substrate material exhibited good solubility, electrical conductivity and large surface. This is beneficial for the further modification of antibodies (Ab) by host-guest interaction and amide bonds. By taking advantage of three-dimensional structure to capture avidin-ALP by amide linkages, PAMAM was used as a catalytic signal amplification element in this assay. Under the optimized condition and at a typical working potential of 0.94 V, the response to 5mC is linear in the 0.01–50 nM concentration range with a detection limit of 3.2 pM (at S/ N = 3). The method is stable, selective and reproducible. It was applied to the determination of 5mC in genomic DNA of human tissue.
An electrochemical immunoassay was constructed for 5-methylcytosine detection based on nanocomposite of graphene oxide, magnetite nanoparticles and β-cyclodextrin, and enzymatic signal amplification.
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ENVIRONMENTAL POLLUTION
·
2019
·
IF 5.714
Bingnan He,Xia Wang,Jianbo Zhu,Baida Kong,Lai Wei,Yuanxiang Jin,Zhengwei Fu
The immunotoxicity of synthetic pyrethroid (SPs) has garnered much attention, and our previous research demonstrated that β-CYP causes immunotoxicity and oxidative stress in macrophages. Nevertheless, the underlying mechanism remains largely unknown. In this study, the murine macrophage RAW 264.7 cells and murine peritoneal macrophages (PMs) were exposed to β-CYP. The results showed that β-CYP elevated intracellular ROS levels in both RAW 264.7 cells and PMs. Exposure to β-CYP also caused mitochondrial dysfunction with reduced mitochondrial membrane potential (MMP), intracellular ATP level and mitochondrial DNA (mtDNA) content in the two cell types. In addition, exposure of RAW 264.7 cells to β-CYP for 12 h and 24 h enhanced autophagy, with elevated Beclin1, Rab7, Lamp1 and LC3-II expression levels, while 48 h of exposure attenuated autophagy. In contrast, exposure of PMs to β-CYP for 12 h promoted autophagy, whereas exposure for 24 h and 48 h impaired autophagy. Cotreatment with an antioxidant, N-acetyl-L-cysteine (NAC), partially blocked the reduced MMP, intracellular ATP level and autophagy disturbance. Moreover, cotreatment with an autophagy agonist, rapamycin (RAPA), partially blocked mitochondrial dysfunction and oxidative stress in the two cell types, whereas cotreatment with an autophagy inhibitor, 3-methyladenine (3-MA), augmented the abovementioned toxic effects. Furthermore, mitochondrial ROS levels in both RAW 264.7 cells and PMs were elevated by exposure to β-CYP, and molecular docking showed that β-CYP docked with mouse respiratory chain complex I by binding to the ND2, ND4, and ND5 subunits of the protein complex. Taken together, the data obtained in the present study demonstrate that oxidative stress partially mediates mitochondrial dysfunction and autophagy disturbance upon exposure to β-CYP in macrophages, and autophagy plays a protective role against the toxic effects.
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JOURNAL OF RADIOANALYTICAL AND NUCLEAR CHEMISTRY
·
2019
·Vol. 322
·期 3
·2033-2042
·
IF 1.186
Li Wei,Liu Huijun,Li Liuxing,Liu Kai,Liu Juan,Tang Ting,Jiang Wenkang
In this paper, a green synthesized citric acid-crosslinked β-cyclodextrin polymer (CA-β-CD) was applied in removing uranium from aqueous solutions. Citric acid acts not only as cross-linker but also as functional groups for providing adsorption sites. The adsorption properties by varying experimental conditions were carried out by batch tests. The maximum adsorption capacity could reach 150 mg g −1 . Adsorption process was fitted better with the pseudo-second-order and Freundlich model. Thermodynamic studies proved that adsorption process was spontaneous and endothermic. More significantly, the polymer showed high selectivity for uranium and excellent reusability, demonstrating that CA-β-CD can be utilized as a promising adsorbent for uranium.
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Food Science & Nutrition
·
2019
·Vol. 7
·期 6
·2043-2049
·
IF 1.747
Yujing Sun,Li Zeng,Yuanzhong Xue,Tianbao Yang,Zongming Cheng,Peilong Sun
β- d -glucosidase can release aroma precursors to improve the flavor of plant food, but the hydrolysis efficiency of the enzyme is low; the purpose of this study was to improve the enzyme activity using ultrasound. The effects of ultrasound parameters on β- d -glucosidase activity were investigated, and the respective structures of enzyme activated and enzyme inhibited were further analyzed. Low temperature (20–45°C), low ultrasonic intensity (<181.53 W/cm 2 ), and short treatment time (<15 min) led to the activation of β- d -glucosidase, whereas high temperature (45–60°C), high ultrasonic intensity (>181.53 W/cm 2 ), and long treatment time (>15 min) led to its inhibition. Application of ultrasound lowered the optimum temperature for β- d -glucosidase activity from 50 to 40°C. Ultrasound did not change the primary structures of the enzyme, but changed the secondary structures. When ultrasound activated β- d -glucosidase, the α-helix contents were increased, the β-fold and irregular coil content were reduced. When ultrasound inhibited β- d -glucosidase, the contents of β-folds were increased, the α-helix and irregular coil contents were reduced.. In summary, activation or inhibition of β- d -glucosidase under ultrasound was determined by the ultrasound conditions. This study suggests that ultrasound combined with β-D-glucosidase can be used in aroma-enhancing.
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RESEARCH ON CHEMICAL INTERMEDIATES
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2018
·Vol. 44
·期 10
·6369-6385
·
IF 1.674
Chen Zhangxu,Fu Ming-Lai,Huang Xiao-Dan,Yuan Baoling,Yang Jia-Cheng E.
To enhance photocatalytic activity spectra area of the TiO 2 based photocatalyst, and to separate and recycle photocatalyst easily, magnetic photocatalyst was prepared with β-NaYF 4 :Yb 3+ ,Tm 3+ , TiO 2 , Fe 3 O 4 as carriers, and tetraethyl orthosilicate as precursor. The magnetic infrared responsive photocatalysts were thoroughly characterized by field emission scanning electron microscopy, X-ray diffraction, a fluorescence spectrometer and UV–Vis–NIR diffuse reflectance spectroscopy. They showed that the composite of β-NaYF 4 :Yb 3+ ,Tm 3+ /TiO 2 /Fe 3 O 4 @SiO 2 (UCTFS) emits visible luminescence upon 980 nm excitation, and energy transfer from β-NaYF 4 :Yb 3+ ,Tm 3+ to TiO 2 was verified. The photocatalytic activities of the UCTFS were studied by orthogonal experiments for photodegradating methylene blue (MB). The degradation rate of MB can reach 86.69% under the optimal conditions. After the four recycles, the degradation rate remained 52.72%. It also showed that 50.27% of the degradation efficiency of phenol can be obtained under the same experimental parameters. These results suggest that magnetic UCTFS composite is stable and a magnetic NIR-driven photocatalyst for degradation of organic pollutants. Moreover, the NIR driven photocatalytic mechanism of this reported composite was proposed. The strategy suggested here may be indicated for the effective fabrication of magnetic NIR-driven photocatalyst for application in the water purification by full spectra of solar energy.
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ENVIRONMENTAL SCIENCE AND POLLUTION RESEARCH
·
2019
·Vol. 26
·期 5
·5094-5110
·
IF 2.914
Zhang Mengjiao,Zhu Liyun,He Changhua,Xu Xiaojun,Duan Zhengyang,Liu Shuli,Song Mingyao,Song Shumin,Shi Jiemei,Li Yu’e,Cao Guangzhu
In this study, the novel adsorbent PVA-TA-βCD was synthesized via thermal cross-linking between polyvinyl alcohol and β-cyclodextrin. The characterization methods SEM-EDS, FTIR, and XPS were adopted to characterize the adsorbent. The effect of pH, contact time, initial concentrations, and temperature during the adsorption of Pb(II), Cd(II), and Mn(II) onto the PVA-TA-βCD was also investigated. In a single-component system, the data fitted well to pseudo-second-order, and film diffusion and intra-particle diffusion both played important roles in the adsorption process. As for isotherm study, it showed a heterogeneous adsorption capacity of 199.11, 116.52, and 90.28 mg g −1 for the Pb(II), Cd(II), and Mn(II), respectively. Competition between the ions existed in a multi-component system; however, owing to the stronger affinity of the PVA-TA-βCD for Pb(II) relative to Cd(II) and Mn(II), the Pb(II) adsorption onto the PVA-TA-βCD was less affected by the addition of the other metals, which could be effectively explained by the hard and soft acid and base theory (HSAB). Furthermore, PVA-TA-βCD showed good reusability throughout regeneration experiments.
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JOURNAL OF AGRICULTURAL AND FOOD CHEMISTRY
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2019
·
IF 3.571
Jin Feng,Lujing Xu,Hua Wang,Songbai Liu
Novel Gemini dodecyl O-glucoside-based primary, secondary, and tertiary vesicles were developed in this work utilizing layer-by-layer deposition of polysaccharides (e.g., sodium carboxymethyl cellulose and chitosan), and their interaction with β-lactoglobulin (BLG) was carefully investigated. The increase of polysaccharide layers on primary vesicles led to a monotonic increase in size and consecutive reversal of surface charge. Polysaccharide deposition significantly retarded the vesicle aggregation and degradation of entrapped catechin laurate during storage. Steady-state fluorescence, isothermal titration calorimetry, and protein precipitation analyses revealed the surface charge dependence of the interactions between vesicles and a model milk protein BLG, which were much stronger when they were charged oppositely than when they presented the same type of surface charge. It was highlighted that the surface charge of vesicles could be tuned by differently charged coatings to accommodate to that of the milk proteins in the food matrix. This work will contribute to the practical application of niosomal vesicles loaded with bioactive compounds to fortify dairy products.
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INDUSTRIAL CROPS AND PRODUCTS
·
2018
·
IF 3.849
Pei Wang,Zhaodi Ke,Juanjuan Yi,Xin Liu,Limin Hao,Qiaozhen Kang,Jike Lu
To enhance the stability of lipases, the additive method is attractive because of its high efficiency and simplicity, of which β-cyclodextrin (β-CD), as an additive has attracted attention. In the present study, the effects of β-CD on enzymatic hydrolysis of hemp seed oil by lipases were investigated. The stability, spectroscopy, and reusability of C andida sp.99–125 were compared by the addition of β-CD, respectively. The results showed that Candida sp.99–125 could produce the highest yield of α-linolenic acid (ALA, 18.27%, w/w) in the presence of β-CD after 24h, and the thermal stability of lipase was also improved. The UV spectroscopy showed that the absorbance of lipase decreased with increasing concentrations of β-CD and the fluorescence result was similar. Moreover, the reusability of lipase with β-CD was better than free lipase. This study revealed that β-CD could increase hydrolysis activity and stability of the lipase, thereby improving the hydrolytic process of hemp seed oil by Candida sp.99–125.
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RSC Advances
·
2018
·Vol. 8
·期 32
·18153-18162
·
IF 2.936
Yawei Chen,Wenjing Song,Xuan Zhao,Qianqian Han,Li Ren
Infectious keratitis is one of the leading causes of blindness in the world, especially in developing countries. Corneal transplant surgery is a feasible treatment for bacterial keratitis when drug therapy cannot be effective. However, the amount of corneal donors is far from requirements of clinical treatment and thus, the prognosis of bacterial keratitis is not satisfactory. In this study, we developed a novel antibacterial corneal repair material (β-CD-DA/OFLX-Col) for bacterial keratitis. The inclusion complex of β-cyclodextrin dialdehyde (β-CD-DA) with ofloxacin formed by host–guest interaction was used as both a drug vector and a crosslinker for further reaction with the amino groups on lysine of collagen chains. Physical properties, cytocompatibility, and antibacterial property of β-CD-DA/OFLX-Col film were characterized. The results indicated that the film was mainly transparent and possessed superior mechanical properties. Moreover, human corneal epithelial cells could adhere to the film and proliferate normally, indicating that the β-CD-DA/OFLX-Col film was non-cytotoxic and had good biocompatibility. Most importantly, the β-CD-DA/OFLX-Col film exhibited prominent antibacterial effects against E. coli and S. aureus in vitro, which could minimize the risks of infection. The prepared β-CD-DA/OFLX-Col film could greatly increase bioavailability of drugs and reduce toxic side effects, thus displaying great potential in bacterial keratitis treatment.
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LUMINESCENCE
·
2018
·Vol. 33
·期 5
·954-961
·
IF 1.671
Penghui Shi,Yan Zhang,Yuhua Li,Liujiao Bian
Both the molecular recognition and interaction of metallo-β-lactamase CcrA with l -captopril were studied by the combined use of fluorescence spectra and molecular dynamic simulation. The results showed that the binding constant was 8.89 × 10 4 L mol −1 at 296 K. Both Zn1 and Zn2 displayed tetrahedral coordination geometries in the CcrA–Lcap complex, the S atom in l -captopril displaced the nucleophilic hydroxide in apo CcrA and occupied the fourth coordination site for each ion, resulting in a competitively inhibited CcrA enzyme. Strong electrostatic interaction between the two zinc ions in CcrA and negatively charged l -captopril provided the main driving force for the binding affinity. Through a partly structural transformation from β-sheet to random coil, loop 1 (residues 24–34) completely opened the binding pocket of CcrA to allow an induced fit of the newly introduced ligand. This study may provide some valuable information for designing and developing a more tightly binding inhibitor to resist superbugs.
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CERAMICS INTERNATIONAL
·
2018
·
IF 3.057
Zhaohui Han,Lei Xu,Chandrasekar Srinivasa Kannan,Jianhua Liu,Sivasankar Koppala,Shaohua Ju,Libo Zhang
In this paper, an Al/TiB 2 layered composite was prepared by plasma spraying for use as the base material to reduce resistivity and improve electrochemical performance in an electrode. The β-PbO 2 coating was prepared by electrochemical deposition . Scanning electron microscopy (SEM) and X-ray diffraction (XRD) were used to study the Al/TiB 2 composite and the β-PbO 2 coating. The electrochemical performance of the Al/TiB 2 /β-PbO 2 and Ti/β-PbO 2 composites was studied by linear sweep voltammetry (LSV), Tafel polarization curves (Tafel) and electrochemical impedance spectroscopy (EIS). The results indicate that Al/TiB 2 /β-PbO 2 composite electrode materials with a uniform structure were successfully prepared by plasma spraying and electrochemical deposition. The structure of the Al/TiB 2 composite improves the conductivity of the electrode matrix, the optimal value of conductivity is 2.76 × 10 7 S m −1 , and the optimal value of density is 8.758 g cm −3 for β-PbO 2 coating. The composite also decreases the charge resistance in the double layer structure of the electrode surface, accelerating the charge transfer rate. Compared with the Ti matrix, the Al/TiB 2 /β-PbO 2 exhibited the best corrosion-resistant performance and electrochemical catalytic activity for electrowinning of nonferrous metals .
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RSC Advances
·
2018
·Vol. 8
·期 55
·31542-31554
·
IF 2.936
Zhengyang Duan,Mingyao Song,Tianguo Li,Shuli Liu,Xiaojun Xu,Ronggao Qin,Changhua He,Yao Wang,Longqian Xu,Mengjiao Zhang
In this study, a crosslinked yeast/β-cyclodextrin polymer (Y–β-CDP), for use as an effective adsorbent for removal Pb(II) and Cd(II) ions from aqueous solution, has been innovatively prepared by grafting β-cyclodextrin (β-CD) onto the surface of baker's yeast (BY) and thiomalic acid as a crosslinker. Several characterization techniques, such as SEM equipped with an EDS analyzer, FTIR, XRD, and XPS were employed characterize the Y–β-CDP. The impact of various operating parameters, such as pH, adsorbent dosage, initial concentration of metal ions, contact time and solution temperature, as well as adsorption kinetics, isotherms and thermodynamics were systematically investigated. The adsorption of Pb(II) and Cd(II) on Y–β-CDP reached equilibrium in 25 min, and the kinetic process conforms to the pseudo-second order model. The Langmuir model was used to describe the adsorption isotherm data better than the Freundlich model. The predicted maximum adsorption capacity at 25 °C for Pb(II) and Cd(II) was 150.08 and 102.80 mg g−1, respectively, when the initial concentration of metal ions was 120 mg L−1. The thermodynamic analysis revealed that the adsorption procedure of Pb(II) and Cd(II) onto Y–β-CDP was spontaneous and endothermic. Furthermore, regeneration experiments demonstrated that Y–β-CDP had excellent recyclability. Together, all results suggested that Y–β-CDP could potentially be a promising adsorbent in the purification of water contaminated with heavy metal ions.
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