INDUSTRIAL & ENGINEERING CHEMISTRY RESEARCH
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2026
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IF 3.9
Yuhan Qiao,Gang Li,Zhiwen Luo,Mengqi Lin
The one-pot synthesis of 1,2-pentanediol from 1-pentene is a route featuring environmental benignity, cost-effectiveness, and high atom economy. In this study, a bifunctional zirconium sulfate-loaded titanosilicate molecular sieve (ZS/Ti-MWW) catalyst was prepared via the impregnation method and used in the one-pot synthesis of 1,2-pentanediol. The effects of reaction conditions, zirconium sulfate loading, and calcination temperature on the catalytic performance for the oxidative hydration of 1-pentene were systematically investigated. On the basis of the NH3-TPD characterization results, the acid amounts of different catalysts were quantitatively analyzed. The results confirmed that zirconium sulfate could introduce abundant acidic sites into the catalyst, and the acid amount varied with the loading amount of zirconium sulfate and the calcination temperature. With the increase in acid sites in the ZS/Ti-MWW catalyst, its catalytic activity in epoxide hydration was markedly improved. Thus, the 1-pentene epoxidation and epoxide hydration were efficiently carried out in one system without additional separation. Under the optimal reaction conditions, the conversion rate of 1-pentene, the selectivity, and the yield of 1,2-pentanediol reached 95.65%, 80.72%, and 77.21%, respectively. This work offers new insights into the design and preparation of bifunctional titanosilicate molecular sieve catalysts as well as the efficient and green synthesis of 1,2-diols from olefins.
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AICHE JOURNAL
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2026
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IF 4.4
Qi Liu,Shanlei Han,Dong Xiong,Yuming Li,Qingxin Yang,Dan Zhao,Yuhang Zhong,Shihao Wang,Zhentao Chen,Dmitry E. Doronkin,Laiyong Zhang,Chunming Xu,Guiyuan Jiang
Tuning hydroxyl defects and constructing active sites at the molecular level is critical for rational design of propane dehydrogenation (PDH) catalysts and understanding the structure–activity relationship. For practical application, enhancing catalysts anti-coking performance is equally important. Herein, the simultaneous regulation of hydroxyl nests content and b -axis thickness was achieved by adjusting the urea/SiO 2 ratio during the preparation of Silicalite-1. The propylene formation rate of the catalysts prepared by introducing Zn species showed a linear positive correlation with the hydroxyl nests content, and the coking behavior was governed by the b -axis thickness of the support. The optimized ZnO x /Silicalite-1 exhibited a high propylene space time yield of 2.2 kg h −1 kg cat −1 with propane conversion of 31% at 550°C. This work provides novel insights into PDH catalyst design from both chemistry and chemical engineering views.
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FREE RADICAL BIOLOGY AND MEDICINE
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2026
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IF 8
Zhan Wang,Cheng Zheng,Wenzhen Li,Kaung Myat Kyaw,Yunmin Zhu,Yihan Lin,Zhen Lin,Di Wu,Zhiqi Hu,Yang Sun,Kaitao Li
Introduction Androgenetic alopecia (AGA), the most common form of hair loss, lacks ideal therapies due to the limitations of current drugs. Natural compounds with low toxicity and good biocompatibility present promising alternatives. Ginkgolide B (GB), a bioactive component of Ginkgo biloba , possesses multiple biological activities, yet its efficacy and mechanism against AGA remain unexplored. Objectives This study aimed to investigate the hair growth-promoting effect of GB in a dihydrotestosterone (DHT)-induced hair loss model and to elucidate the underlying molecular mechanism. Additionally, a novel detachable microneedle delivery system was developed and evaluated to overcome GB's poor transdermal permeability, thereby enhancing its therapeutic efficacy. Methods The AGA microenvironment was first simulated using DHT-stimulated human dermal papilla cells (HDPCs) in vitro. The molecular mechanism by which GB improves the secretory function of HDPCs was investigated through network pharmacology, western blot, transmission electron microscopy, and immunofluorescence. Subsequently, a detachable microneedle system was designed, in which GB was encapsulated in poly (lactic-co-glycolic acid) (PLGA) microspheres and integrated with the sulfobutylether-β-cyclodextrin (SCD)-based matrix (GB@MS-MN). The therapeutic efficacy of this system was evaluated in a DHT-induced hair loss model. Results GB rescued DHT-induced dysfunction in HDPCs, reversing the aberrant secretion of hepatocyte growth factor (HGF) and dickkopf-1 (DKK1). Mechanistically, GB alleviated DHT-induced endoplasmic reticulum stress (ERS) in HDPCs by activating the nuclear factor erythroid 2-related factor 2 (Nrf2)/heme oxygenase-1 (HO-1) pathway. And GB promoted hair growth in DHT-induced hair loss mice. The GB@MS-MN system demonstrated excellent mechanical properties and targeted follicular delivery and showed superior hair growth promotion compared to free GB and minoxidil. It effectively suppressed follicular ERS, with no observed systemic toxicity. Conclusion In summary, this study confirms that GB protects HDPCs function by suppressing DHT-induced ERS through activation of the Nrf2/HO-1 pathway. Furthermore, a detachable microneedle delivery system based on a PLGA-SCD composite effectively overcomes the transdermal barrier of GB and demonstrates superior hair-promoting efficacy.
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ECOTOXICOLOGY AND ENVIRONMENTAL SAFETY
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2026
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IF 6.6
Chuanlong Huang,Mengqi Hao,Shurun Yao,Lirong Wang,Gang Liu,Zhuo Wei
Bioreduction of Cr(VI) by Shewanella through extracellular electron transmission (EET) attracts increasing interest due to cytochrome c (cyts c ) system especially conductive flagella (“nanowire”) structure. The responses of bioreduction efficiency, “nanowire” growth, cyts c synthesis, and e (electron) transmission intensity through CymA→MtrA→MtrB→MtrC→OmcA (Route 1) and CymA→NapA→NapB (Route 2) to iron ion (Fe 3 + ) and nitrate (NO 3 - ) were evaluated. Results showed that Fe 3+ and NO 3 - of 1–1.5 mM significantly ( p < 0.01) enhanced bioreduction of Cr(VI) from 67% to 82% and 79%, respectively with promoted cyts c -mediated c onductivity (C p ). Both Fe 3+ and NO 3 - stimulated the growth of “nanowire” and synthesis of cyts c participating in EET with Fe 3+ showing stronger stimulation on Routes 1 while NO 3 - on Routes 2. The genes expression of cyts c on inner membrane (CymA), periplasmic space (NapA/NapB), and “nanowire” (MtrA/MtrB/MtrC, OmcA) show synchronous responses to Fe 3+ and NO 3 - . However, contents of mobile cyts c in extracellular polymeric substances (EPS) and structural cyts c in cell membrane showed inconsistent responses to Fe 3+ and NO 3 - . Overall, this study illustrated the regulation mechanism of e acceptors on cyts c- electron transmission system of Shewanella , which help improving the electrochemical efficiency on Shewanella and provide novel idea in chromium remediation strategy .
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Advanced Science
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2026
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IF 14.1
Kaiyuan Jiang,Fen Xiong,Yiwen Peng,Lingfei Meng,Xinran Wang,Yingruo Xu,Tian Tang,Hongchang Gao
Diabetic cardiomyopathy (DCM) is a major cardiovascular complication of diabetes with limited effective interventions. Using a streptozotocin-induced insulin-deficient, type 1 diabetes-like DCM mouse model, we show that intermittent fasting (IF) improves cardiac function and attenuates myocardial remodeling. Antibiotic-mediated microbiota depletion largely abolished these benefits, whereas fecal microbiota transplantation from IF-treated donors recapitulated cardioprotection, supporting a causal role of the gut microbiota. Metagenomic profiling identified Akkermansia muciniphila ( A. muciniphila ) as a prominent IF-responsive taxon, and A. muciniphila supplementation alleviated cardiac injury without obvious improvement in glycaemia. Integrated serum and heart metabolomics identified 1-methyl-L-histidine as a microbiota-associated metabolite reduced in diabetes but restored by IF and A. muciniphila . In vitro and ex vivo assays further supported an L-anserine-linked microbial route for 1-methyl-L-histidine generation. Importantly, oral 1-methyl-L-histidine supplementation recapitulated key cardioprotective effects, remodeled cardiac lipid homeostasis, and reduced lipid peroxidation and oxidative injury. Together, these findings support a gut microbiota–metabolite–lipid axis associated with IF-related cardioprotection in DCM and highlight microbial metabolites as tractable targets to complement dietary intervention.
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INTERNATIONAL JOURNAL OF BIOLOGICAL MACROMOLECULES
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2026
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IF 8.7
Bin Yan,Hongru Zhao,Bingmei Su,Juan Lin
Protein engineering altered the reaction direction of TsRTA for AP. • Adjusting the catalytic center and entrance of TsRTA promoted AP binding. • A 10-site variant of TsRTA enabled biosynthesis of ( R )-1-PEA. • An acetone elimination module increased Mu10 productivity by 57.4%. ( R )-1-Phenylethylamine [( R )-1-PEA] is an important chiral compound widely used in pharmaceuticals, chemical synthesis, and materials science. ω-Transaminases are valuable biocatalysts for the asymmetric amination of ketone substrates under mild conditions and represent an attractive tool for the green synthesis of chiral amines. However, in natural ( R )-selective transaminases, acetophenone (AP) is more commonly produced as the deamination product of ( R )-1-PEA, whereas its amination as an amino acceptor is generally inefficient. In this study, an ( R )-selective ω-transaminase from Thermomyces stellatus (TsRTA), which originally catalyzed only the deamination of ( R )-1-PEA to AP, was selected as the engineering target. Protein engineering was performed to shift its reaction equilibrium, thereby enabling the asymmetric synthesis of ( R )-1-PEA from AP. To overcome the thermodynamic limitation caused by acetone accumulation, an “acetone elimination–cofactor regeneration” coupled module was introduced to improve the whole-cell catalytic efficiency for ( R )-1-PEA synthesis using AP as the substrate. Under optimal reaction conditions (35 °C, pH 10.0, 0.5 mM PLP, an isopropylamine/AP molar ratio of 4:1, 200 mM formate, and 0.2 mM NAD + ), the whole-cell catalytic system produced 67 mM ( R )-1-PEA from 100 mM AP within 8 h. This study lays a foundation for the scalable preparation of ( R )-1-PEA and provides a useful reference for regulating the reaction direction of transaminases. Download: Download high-res image (205KB) Download: Download full-size image
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SEPARATION AND PURIFICATION TECHNOLOGY
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2026
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IF 9.1
Jiaozhong Cai,Jiacheng Li,Jiushuai Deng,Bozeng Wu,Ni Xiao,Junnan Chen,Wei Weng,Xiaopeng Chi,Wen Tan,Guangsheng Zeng,Shuiping Zhong
Selective depression of chalcopyrite while preserving molybdenite floatability remains challenging. Conventional sulfur-containing depressants may oxidize readily, require high dosages, or depend on strong alkalinity, prompting the development of new selective reagents. Sodium 3-mercapto-1-propanesulfonate (MPS), containing thiol and sulfonate groups, was introduced as a selective chalcopyrite depressant. At pH 7 and 1.5 × 10 −5 mol L −1 MPS, the maximum recovery difference in single-mineral flotation reached 87.6 percentage points. In artificial mixed-mineral flotation, Cu recovery decreased to 7.28%, whereas Mo recovery remained at 91.16%, increasing the separation index from 1.28 to 11.49. Contact angle, SEM–EDS, and FTIR analyses indicated preferential retention of MPS-related species on chalcopyrite, together with interaction between thiol S and surface Cu sites and enhanced surface hydrophilicity associated with the sulfonate group. Under the electrochemical test conditions, MPS attenuated the anodic response and reduced the corrosion current density of chalcopyrite by approximately 40%, with little effect on molybdenite. DFT calculations further identified the Cu site on chalcopyrite (112) as more favorable for MPS adsorption than the Fe site (−100.96 versus −86.02 kJ mol −1 ), whereas the modelled adsorption configuration on molybdenite (001) was energetically unfavorable (+47.61 kJ mol −1 ). This study elucidates a selective depression mechanism involving preferential adsorption, increased surface hydrophilicity, and altered chalcopyrite redox behavior, while demonstrating the potential of MPS for near-neutral Cu Mo flotation separation. Download: Download high-res image (142KB) Download: Download full-size image
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Translational Cancer Research
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2026
·Vol. 15
·期 8
·585
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IF 2.1
Gennian Wang,Hua Cheng,Yaping Zhang,Xiaohu Guo,Yumin Li
Background Microplastics and nanoplastics (MNPs), as emerging environmental pollutants, have garnered significant attention from the global scientific community due to their potential threats to human health, particularly their association with the occurrence and development of cancer. The goal of our study is to create a predictive marker for pancreatic ductal adenocarcinoma (PAAD) based on MNPs-related genes, with the purposes of predicting survival outcomes and assessing the tumor immune microenvironment. Methods Using multi-cohort data from The Cancer Genome Atlas (TCGA), Gene Expression Omnibus (GEO), and International Cancer Genome Consortium (ICGC), we assessed the association between MNPs and PAAD prognosis through the Xiantao Academic (https://www.xiantao.love/). The development of a prognostic signature was followed by an assessment of its significance through the Kaplan-Meier method, time-dependent receiver operating characteristic (ROC), and decision curve analysis (DCA). The validity of the risk model was confirmed through the ICGC and GSE71729 cohorts. The model was then assessed for levels of tumor immune infiltration. To explore MNPs-related genes expression characteristics within immune cells in PAAD, we performed single-cell RNA sequencing and spatial transcriptomics analysis through the Sparkle Platform ( https://grswsci.top/ ). Finally, in vitro experiments were conducted to investigate the biological function of interleukin 1 alpha (IL1A). Results A four-gene signature comprising XDH, IL1A, KIF20A, and ASPM, based on MNPs, was developed to stratify PAAD patients into two distinct risk groups. The high-risk group showed a significantly poorer prognosis. A similar trend was verified in the external cohorts ICGC and GSE71729 . The signature risk score affected immune cell infiltration in the PAAD microenvironment. The infiltration of B cells, CD8 + T cells, cytotoxic cells, immature dendritic cells (iDCs), mast cells, plasmacytoid dendritic cell (pDC), T cells, Tem cells, T follicular helper (TFH) cells, and T helper 17 (Th17) cells had a positive correlation with the low-risk group. In contrast, high-risk patients tended to have increased number of T helper (Th2) cells and higher expression of SIGLEC15, CD274, IGSF8. Knockdown of IL1A in PAAD cells inhibited their tumor proliferation ability in vitro . Conclusions Using MNPs-related genes, we built a prognostic model for PAAD, revealing that patients with high-risk scores are likely to have a worse prognosis. This model is designed to develop personalized treatment strategies tailored to the specific needs of each patient, thereby improving clinical outcomes for PAAD patients. Furthermore, IL1A could be a promising therapeutic candidate for PAAD.
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ANALYTICA CHIMICA ACTA
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2026
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IF 6.1
Zimiao Wen,Xinhua Lian,Zihan Zhang,Haiyan Zhao,Na Li,Min Cui,Cong Zhang
Insulin-like growth factor-1 (IGF-1) is a critical biomarker linked to various human diseases, necessitating sensitive and reliable detection methods. Herein, we report a novel sandwich-type ratiometric electrochemical immunosensor with signal on–off capability for the ultrasensitive measurement of IGF-1. The sensor was constructed using gold nanoparticle-engineered carbonized covalent organic frameworks (Au@C-COFs) as the sensing platform and thionine-enriched alkynyl COFs (Thi@aCOFs) as the signal amplification probe. Au@C-COFs exhibited excellent electrocatalytic activity toward hydroquinone (HQ) oxidation, providing a stable internal reference signal, while Thi@aCOFs generated a concentration-dependent reporter signal upon specific recognition of IGF-1 via the immobilized aptamer. The ratiometric strategy effectively eliminated external interferences, significantly enhancing accuracy and reliability. Under optimal conditions, the biosensor displayed a wide linear range from 10 fM to 1 nM, a low detection limit of 0.45 fM (S/N = 3), good specificity, stability, and satisfactory reproducibility. Furthermore, the sensor performed well for measuring IGF-1 in diluted human serum, achieving satisfactory recoveries. This work presents a robust and adaptable platform toward ratiometric electrochemical biosensing, holding great promise to achieve clinical diagnosis and management treatment of IGF-1-related disorders.
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Antioxidants
·
2026
·Vol. 15
·期 9
·1114
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IF 8.2
Wen-Xu Yao,Yao-Dan Ma,Shi-Yao Ding,Jian Lu,Hao-Lan Tang,Zeng-Ming Yang
Successful embryo implantation requires intimate crosstalk between the blastocyst and the uterine epithelium within a defined window of receptivity. However, the metabolic signals that mediate this process in mammals remain poorly understood. In this study, pregnant mice, primary uterine cell culture and uterine epithelial organoids were used to examine the regulation and function of heme oxygenase-1 (HO-1) during mouse embryo implantation and decidualization. We demonstrate that embryo-derived lactic acid drives heme catabolism and regulates epithelial receptivity in mice through a hypoxia-inducible factor 1α (HIF1α) -heme oxygenase-1 (HO-1) signaling axis. Specifically, lactic acid stabilizes HIF1α to induce HO-1 expression in uterine epithelial cells by promoting von Hippel-Lindau (VHL) nucleolar sequestration and downregulating PHD2/3. Additionally, lactic acid suppresses the transcriptional repressor BACH1, further facilitating HO-1 induction. At physiological heme levels, HO-1-derived bilirubin promotes epithelial receptivity by increasing phosphorylated STAT3 (p-STAT3) and downregulating MUC1. A low dose of hemin promotes epithelial receptivity and decidualization, whereas a high dose of hemin suppresses these processes. Pharmacological inhibition of HO-1 in mice markedly reduces implantation sites, establishing the functional necessity of this pathway. However, when heme levels exceed the regulatory capacity of HO-1, epithelial dysfunction ensues, characterized by reduced p-STAT3 and elevated MUC1, which ultimately disrupts implantation. Consistent with this, chronic heme exposure by oral gavage in mice increases uterine heme levels and upregulates BACH1, thereby suppressing HO-1 and trapping the uterus in a non-receptive state, causing implantation failure. Our findings define a lactic acid-HIF1α-HO-1-heme metabolic checkpoint that couples glycolytic signaling to heme regulation and endometrial receptivity. Dysregulation of this checkpoint may contribute to implantation disorders associated with heme stress, providing mechanistic insights into heme stress-related uterine receptivity failure.
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JOURNAL OF THE AMERICAN SOCIETY FOR MASS SPECTROMETRY
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2026
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IF 2.9
Jin Xu,Anqi Zhou,Mengjiao Xu,Qiaoshuang Chen,Chenjing Xu,Jinhao Li,Na Li,Zhixin Li,Guodong Wu,Weitao Zhang,Shuhai Xu,Qiaoling Ni,Tao Liu,Chunguang Zheng,Wei Yu,Xi Chen,Lankun Song,Min Xia,Qingcheng Guo,Lusha Ji,Weizhu Qian,Sheng Hou,Jun Li,Ziqiao Sun,Xuekun Wang,Fubo Han,Hao Wang,Jing Li,Dapeng Zhang,Huaizu Guo
Fc-containing GLP-1 therapeutics exhibit complex post-translational modification (PTM) heterogeneity, necessitating advanced analytical methods for quality control (QC) and process analytical technology (PAT). We developed a reverse-phase liquid chromatography (RP-LC) method for the PTM-specific profiling of these biologics. Using dulaglutide (IgG4-Fc) as a model, critical parameters─including mobile-phase additives, acid concentration, and shallow gradients─were optimized to resolve PTM variants (e.g., hydroxylation, N-terminal truncation, disulfide reduction, glycosylation) within 40 min. Mass spectrometry (MS) compatibility was enabled by adopting difluoroacetic acid (DFA) as an alternative ion-pairing reagent to support intact-mass characterization of variants. This enabled the identification of additional PTMs not readily resolved under the initial RP-LC conditions, including site-specific HyK-Gal-Glc O-glycosylation and process-dependent truncations. The method also allowed for the direct quantification of critical impurities and the detection of process-induced variants across biosimilar clones. The method was further demonstrated on the IgG2-subtype GLP-1-Fc-fusion (supaglutide), showing applicability across the two Fc subtypes examined without additional optimization. This robust, MS-compatible RP-LC platform provides a rapid, accurate, and comprehensive (RAC) means of conducting PTM-specific QC for Fc-GLP-1 therapeutics, supporting PAT implementation and accelerating biosimilar and next-generation drug development.
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MICROCHIMICA ACTA
·
2026
·Vol. 193
·期 6
·384
·
IF 5.4
Xu Ziyu,Cao Jinru,Wang Ying,Chen Tingsha,Wang Yue,Xu Huaqiu,Zeng Huihui,Huang Wenyi,Cheng Hao,Kong Hongxing,Lu Yao,Feng Jun
Human carboxylesterase 1 (hCE1) is a serine hydrolase that is distributed widely in the human liver, intestines, and various tumour tissues. It has now become a biomarker associated with diseases such as cancer. Consequently, the measurement of hCE1 levels in human serum is of significant importance for the diagnosis and assessment of these diseases. The present study involved the preparation of Ag/CQDs/ZIF-8 nanocomposites, which incorporated silver nanoparticles (Ag NPs) and carbon quantum dots (CQDs) into the ZIF-8 framework. The combination of this composite with an enzyme-linked immunosorbent assay (ELISA) and the utilisation of benzidine as the signal molecule resulted in the development of a fluorescence (FL)/surface-enhanced Raman scattering (SERS) dual-mode sensor, which was employed for the sensitive detection of hCE1 in serum. This dual-mode sensor combines the advantages of both FL and SERS technologies, demonstrating excellent selectivity and stability, and enhancing the accuracy and reliability of quantitative measurements. The limits of detection (LOD) for the FL mode and SERS mode were determined to be 0.1 and 0.072 ng·mL − 1 , respectively, with the recoveries ranging from 92.44% to 117.47%. The satisfactory outcomes obtained from testing on real sample confirm the sensor’s excellent suitability for use in complex biological matrices. Consequently, this dual-mode sensor shows considerable promise for the detection of biomolecules in clinical samples, thereby demonstrating significant potential for clinical applications. Graphical abstract The alternative text for this image may have been generated using AI.
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