CHEMICO-BIOLOGICAL INTERACTIONS
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2019
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IF 3.407
Wen-Jing Wu,Wei-Feng Tang,Ming-Hui Xiang,Jianshe Yan,Xiumei Cao,Chang-Hui Zhou,Yan Chang,Jing Xi,Yi-Yi Cao,Yang Luan,Xin-Yu Zhang
1-Chloro-2-hydroxy-3-butene (CHB) is a possible metabolite of 1,3-butadiene, a carcinogenic air pollutant . To demonstrate its formation in vivo, it is desirable to develop a practical biomarker and the corresponding analysis method. CHB can undergo alcohol dehydrogenase- and cytochromes P450 enzymes (P450)-mediated oxidation to yield 1-chloro-3-buten-2-one (CBO), which readily forms glutathione conjugates. We hypothesized that CBO-derived mercapturic acids , which are the expected biotransformed products of CBO-glutathione conjugates, could be used as CHB biomarkers. Thus, in the present study, we investigated the in vivo biotransformation of CHB into CBO-derived mercapturic acids. Because the reaction of CBO with N -acetyl- l -cysteine yields two products, 1,4- bis ( N -acetyl- S -cysteinyl)-2-butanone (NC1) and 1-chloro-4-( N -acetyl- S -cysteinyl)-2-butanone (NC2), we first developed an isotope dilution LC/ESI − -MS-MS method to quantitate urinary NC1 and NC2, and then determined their concentrations in urine of C57BL/6 mice and Sprague-Dawley rats administered CHB. Since no NC2 was detected in samples, the LC/ESI − -MS-MS method was optimized specifically for NC1. NC1 was enriched through solid phase extraction with the recovery being 75–82%. The limits of detection and quantitation were 6.8 and 34 fmol/0.1 mL for mouse urine, and 4.5 and 7.1 fmol/0.1 mL for rat urine, respectively. In urine of animals before CHB administration, no NC1 was detected; in mice administered CHB at 10 and 30 mg/kg, and rats at 5 and 15 mg/kg, NC1 was detected and its concentrations in urine from animals given higher doses were 3–6 fold higher than those given lower doses. Moreover, the NC1 concentrations in urine during 0–8 h were 4–6 fold and 10–11 fold higher than those during 8–24 h for mice and rats, respectively. The results demonstrated that CHB could be in vivo biotransformed into NC1, which could be used as a practical CHB biomarker.
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高影响因子
JOURNAL OF APPLIED POLYMER SCIENCE
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2015
·Vol. 132
·期 18
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IF 1.768
Hongchuan Wu,Zhiguo Li,Libin Zhu,Jiyou Gu
Aqueous polyurethane micelles from 2,4,6-trichlorphenol blocking terminal-isocynate pre-polymer were prepared which could be de-blocked at low temperature. The prepared aqueous blocked polyurethane micelles (BPUMs) were uniform and spherical micro-particles. The particle size was decreased with increase of the DMPA content, while it was enlarged at first and then gradually decreased by increasing R -value. The stability of the micelles was affected by the R value, blocked efficiency and DMPA content. HDI-BPUMs were more stable than HDI-BPUMs at the same condition. The stability was further researched by the zeta potential combining with technique and viscosity tracking method, and the initial zeta potential be used as the stability criterion. In addition, a stabilization mechanism of the micelles was proposed. TCP-blocked TDI-BPUMs could be initially de-blocked at about 75°C and TCP-blocked HDI-BPUMs was de-blocked at about 80°C. The de-blocking temperature could be further reduced in the presence of catalyst, but it was scarcely affected by the change of R -value and DMPA content. © 2015 Wiley Periodicals, Inc. J. Appl. Polym. Sci. 2015 , 132 , 41908
研究领域:
高分子化学
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胶体与界面科学
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材料化学
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功能材料
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纳米材料
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JOURNAL OF HAZARDOUS MATERIALS
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2026
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IF 10.6
Ruomu Chen,Yumeng Niu,Nan Li,Jingrui Peng,Ziqian Xia,Chenyang Wang,Jiandong Jiang,Kai Chen
Nitro-polycyclic aromatic hydrocarbons (nitro-PAHs) are toxic and persistent environmental contaminants of increasing concern because of their widespread occurrence and ecological risks. Nitronaphthalene, a representative nitro-PAH, occurs as positional isomers, yet microbial degradation studies have mainly focused on 1-nitronaphthalene (1-NN), leaving the basis for biodegradation of 2-nitronaphthalene (2-NN) largely unresolved. In this study, Burkholderia pseudomultivorans M3 was isolated from river sediment in Anhui Province, China, and was found to efficiently degrade both 1-NN and 2-NN, indicating an expanded degradation range for nitronaphthalene isomers. Genome analysis identified a novel nitronaphthalene degradation gene cluster ( nnd ) that is phylogenetically related to reported nin - and nag -type gene clusters but differs functionally from both. Gene knockout and complementation experiments demonstrated that nndAc , encoding the catalytic α-subunit of a Rieske-type dioxygenase, is essential for degradation of both isomers. Heterologous expression and metabolite identification further showed that the NndAB system transforms 1-NN and 2-NN into 1,2-dihydroxynaphthalene via the corresponding nitro-dihydrodiol intermediates, with concomitant nitrite release. Site-directed mutagenesis revealed that the ability of NndAc depends on the coordinated effects of multiple active-site residues, with F200 being particularly important for 2-NN transformation. Moreover, strain M3 maintained substantial removal efficiency in synthetic wastewater containing mixed nitro-, carboxyl-, and hydroxyl-substituted naphthalene compounds, supporting its applicability in complex contaminated matrices. These findings reveal the molecular basis underlying the broadened substrate range of nitronaphthalene degradation and highlight the potential of strain M3 for remediation of wastewater containing mixed substituted naphthalene pollutants.
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AQUACULTURE
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2026
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IF 4.4
Jialong Huang,Guohao Chen,Tingyu Wei,Xuemin Wei,Yinghui Wang,Aiguo Huang
Streptococcus agalactiae (Group B Streptococcus, GBS) is one of the most harmful pathogens in aquaculture and its virulence is mainly regulated by the LuxS/AI-2 quorum sensing (QS) system. Developing quorum sensing inhibitors (QSIs) targeting the GBS LuxS protein is not prone to inducing drug resistance, which is of great significance for addressing the antimicrobial resistance crisis in aquaculture. This study identified naringin, a potential LuxS inhibitor with anti-GBS virulence activity, from plant-derived natural products through virtual screening combined with experimental verification. The results showed that naringin bound tightly to the entrance of the GBS LuxS active pocket and effectively inhibited luxS gene transcription. This dual action may reduce AI-2 signal molecule synthesis through both potential steric hindrance at the active site and transcriptional inhibition. At sub-inhibitory concentrations, naringin did not inhibit bacterial growth. Instead, it downregulated key virulence genes, significantly suppressed EPS secretion, and disrupted the biofilm barrier, thereby attenuating bacterial virulence. The results of in vivo challenge experiments showed that naringin intervention significantly alleviated histopathological damage in infected hosts and effectively improved the survival rate after infection. This protective effect may be closely associated with the ability of naringin to inhibit the colonization and dissemination of GBS in the host spleen and brain tissues, while downregulating host pro-inflammatory gene expression and upregulating antioxidant gene expression. This study confirmed that naringin can effectively disrupt the GBS LuxS/AI-2 quorum sensing system and exert significant anti-virulence effects. It also provides a new candidate drug and theoretical basis for the green prevention and control of GBS infection in aquaculture.
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Frontiers in Microbiology
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2026
·
IF 5.8
Nesreen Aljahdali
2-Amino-1-methyl-6-phenylimidazo[4,5-b]pyridine (PhIP) is a heterocyclic amine (HCA) that is formed during high-temperature meat cooking, especially grilling, and is classified as a carcinogenic food-borne compound that can impact multiple physiological systems. The aim of this study was to evaluate how exposure to PhIP affects the composition of the cecal microbiota in mice. A total of 32 mice were randomly divided into four groups: A negative control group, a positive control group, and two groups treated orally with either 10 or 20 mg/kg PhIP for 2 months. The V3-V4 regions of the bacterial 16S rRNA gene were sequenced to characterize the cecal microbial communities. Microbial richness and diversity decreased in the PhIP-treated mice compared to the negative control group. At the phylum level, the relative abundance of Bacillota and Actinomycetota decreased significantly after PhIP exposure, while the abundance of Campylobacterota and Bacteroidota increased. At the genus level, the abundance of Roseburia, Lactobacillus, and Bifidobacterium decreased, while the abundance of Blautia, Lachnospiraceae-NK4A136 group, Bacteroides, Alistipes, Helicobacter, and Escherichia–Shigella increased. The results highlight that bacterial community composition and its relative abundance in the cecum change with PhIP exposure and offer further evidence of microbiota dysbiosis that occurs with PhIP exposure.
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JOURNAL OF PHOTOCHEMISTRY AND PHOTOBIOLOGY A-CHEMISTRY
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2026
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IF 4.4
Tingyang Ming,Miao Hu,Wei Zhu
Non-equilibrium adsorption contributes to post-illumination TC removal. • Bi 2 WO 6 /Ni(OH) 2 shows enhanced adsorption and photocatalytic kinetics. • Charge storage is unlikely to dominate the dark-stage behavior. • Acid extraction reveals adsorption-related surface retention of TC. • Light-off timing supports adsorption relaxation after photocatalysis. The photocatalytic removal of antibiotic pollutants has been widely investigated for water purification, where continued pollutant decay after light irradiation (“dark-stage removal”) represents an intriguing post-illumination phenomenon in photocatalytic systems. Although charge storage provides an important explanation for post-illumination activity, adsorption relaxation after light-off may also contribute to dark-stage removal. In this work, a Ni(OH) 2 -modified Bi 2 WO 6 system was employed as a representative model to investigate non-equilibrium adsorption in photocatalytic dark-stage tetracycline removal. The photocatalytic performance under visible light was first evaluated to establish a high-activity reaction system. Subsequently, a combination of acid extraction, solution–surface distribution analysis, and charge probing experiments was used to clarify the contributions of adsorption redistribution and charge storage. The results demonstrate that charge storage is negligible and unlikely to dominate the dark-stage behavior in the Bi 2 WO 6 /Ni(OH) 2 system. Instead, the apparent dark-stage removal is primarily governed by a non-equilibrium adsorption process, arising from the imbalance between rapid photocatalytic consumption and adsorption–desorption dynamics during illumination. After light-off, the system gradually relaxes toward equilibrium, leading to continued redistribution of tetracycline from the solution to the catalyst surface. This work reveals a photocatalysis-induced non-equilibrium adsorption pathway for dark-stage removal, providing a complementary perspective for understanding post-illumination behavior in photocatalytic systems. Download: Download high-res image (181KB) Download: Download full-size image
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HYDROMETALLURGY
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2026
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IF 6.3
Qianshun Wen,Yuefeng Deng,Ji Chen,Shuang Sun
Yttrium (Y) is extensively utilized in various industries for its excellent optical, electrical, and magnetic properties. However, the separation between Y and other rare earth elements (REEs) is difficult, because the properties of Y are very similar to other REEs. To overcome the drawbacks of traditional extractants, such as complex structural composition and poor stability, it is important to develop new extractants for separating Y. Featured in this study is a novel carboxylic acid extractant, 2-hexyldecanoic acid (DY319, HA), employed for extraction and separation of Y. The pK a value of DY319 is 7.54. The extraction sequence of REEs with DY319 is as follows: Lu > Yb > Eu > Tm > Er > Ho ≈ Tb > Dy > Pr > Gd > Nd ≈ Sm > Ce > Y > La. Additionally, the extraction parameters, including the extractant concentration, saponification degree, phase volume ratio, equilibration time, and temperature, were carefully studied. The separation factors of separating Y from other REEs were measured, and the separation factors between Y and Ho, Er, Tm, Yb, Lu are 2.37, 2.46, 3.05, 4.05, and 4.12, respectively. Moreover, it exhibits excellent recyclability and loading capacity. Quantitative stripping can be accomplished with HCl by single-stage stripping. Through a cascade extraction process with 16-stage extraction and 4-stage scrubbing, the Y product with 99.9 wt% purity was obtained. Ultimately, the extraction process is governed by the cation exchange reaction, and the extraction complex was deduced to be REA 3 .
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INDUSTRIAL & ENGINEERING CHEMISTRY RESEARCH
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2026
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IF 3.9
Leibing Chen,Kairui Li,Jing Li,Xinwei Du,Haisheng Wei
The development of highly dispersed supported noble-metal catalysts is crucial for maximizing atomic utilization and enhancing catalytic performance. This work demonstrates a highly efficient Au–Pt bimetallic catalyst supported on 2-methylimidazole-modified ZnO (N-ZnO) for the chemoselective hydrogenation of nitroarenes. The modification creates strong anchoring sites for metal precursors, which, as confirmed by DFT calculations, effectively suppress metal aggregation and yield highly dispersed nanoparticles with an average size of 2.9 nm. The resulting Au–Pt/N-ZnO catalyst exhibits exceptional performance in the hydrogenation of p-chloronitrobenzene under mild conditions (50 °C and 0.5 MPa H2), achieving >99% conversion and 98.6% selectivity to p-chloroaniline, significantly outperforming its monometallic counterparts due to the synergistic effect. The catalyst also exhibited excellent recyclability and broad substrate applicability for various substituted nitroarenes. This work provides an effective strategy for fabricating highly efficient supported bimetallic catalysts through the organic ligand-mediated surface modification of metal oxide supports.
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INTERNATIONAL IMMUNOPHARMACOLOGY
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2026
·
IF 5.6
Xizhi Li,Tingting Zhou,Yixi Wu,Jiayi Sun,Ziyu Wang,Yuhan Huang,Ke Xu,Hongwei Ling,Na Li,Tingting Yang,Tao Wang
Exenatide, the first glucagon-like peptide-1 receptor agonist (GLP-1RA), exerted multiple beneficial effects, including stimulating insulin secretion, slowing gastric emptying, and improving insulin resistance (IR). Our previous work demonstrated that the peroxisome proliferator-activated receptor delta (PPARδ) gene ( PPARD ) polymorphisms correlated with exenatide monotherapy efficacy, possibly due to the pivotal role of PPARδ in regulating IR. Specifically, PPARδ has been shown to modulate the nucleotide-binding domain and leucine-rich repeat pyrin domain-containing 3 (NLRP3) inflammasome, a key regulator of pyroptosis, thereby participating in the regulation of this inflammatory cell death pathway. However, the underlying mechanisms by which exenatide ameliorated hepatic IR in patients with type 2 Diabetes Mellitus (T2DM) remained incompletely understood. Herein, we found that exenatide suppressed pyroptosis and ameliorated hepatic IR; furthermore, it could bind to PPARδ and upregulate PPARδ protein expression both in vitro and in vivo. Notably, PPARδ knockdown abolished the protective effects of exenatide against pyroptosis and hepatic IR, whereas pharmacological activation of PPARδ enhanced these beneficial effects. Moreover, T2DM patients carrying the AA genotype at PPARD rs3777744 and exhibiting higher baseline homeostasis model assessment of insulin resistance (HOMA-IR) showed a superior response to exenatide. Collectively, our findings revealed that exenatide ameliorated hepatic IR by suppressing pyroptosis via PPARδ, underscoring the potential of PPARD rs3777744 as a biomarker for personalized exenatide therapy in T2DM.
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JOURNAL OF ELECTROANALYTICAL CHEMISTRY
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2026
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IF 4.6
Sheng Liang,Zhihao Wang,Kunhong Hu,Lei Hu,Lili Wang,Lingli Liu,Ningning Zhou,Jiage Yu,Bin Chen,Xin Liang
Lithium‑sulfur (Li S) batteries based on lithium sulfide (Li 2 S) cathodes hold great promise for next-generation energy storage devices but suffer from critical drawbacks, including lithium polysulfides (LiPSs) shuttling, sluggish redox reaction kinetics, and severe safety hazards. Here, a multifunctional separator is designed by coating a nickel hydroxide (Ni(OH) 2 ) nanoflakes-decorated kelp-like porous carbon (Ni(OH) 2 /KLPC) composite onto a polypropylene (PP) membrane. The unique KLPC skeleton derived from mucor hyphae provides a three-dimensional conductive network, while the polar Ni(OH) 2 nanoflakes offer strong chemisorption for LiPSs and high catalytic activation for the interconversion between solid-phase (Li 2 S and S) and liquid-phase (LiPSs). Meanwhile, the Ni(OH) 2 nanoflakes endow the separator with remarkable flame retardancy through an endothermic decomposition process and the formation of highly flame-retardant products nickel oxide and water. Consequently, the Li S battery assembled with the Ni(OH) 2 @KLPC modified PP (Ni(OH) 2 /KLPC/PP) separator and a Li 2 S/multi-walled carbon nanotube cathode delivers a high initial discharge capacity of 1132 mAh g −1 , remarkable rate property of 320 mAh g −1 at 5.0 A g −1 , and stable long-term cycling over 750 cycles. This work presents a promising synergistic strategy for constructing high-performance and safe Li S batteries through multifunctional separator engineering.
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MICROCHEMICAL JOURNAL
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2026
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IF 5.2
Qing Huang,Zefeng Hou,Yunong Zhao,Ruiqin Gu,Chengli Li,Minghong Yang,Huan Liu
Surface plasmon resonance (SPR) technology, boasting prominent advantages including anti-electromagnetic interference, high sensitivity, rapid response, label-free detection, and minimal sample consumption, holds broad application prospects in life sciences, clinical diagnostics, drug screening, and other related fields. However, current optical fiber biochemical sensors are generally plagued by poor probe practicality and stability, low refractive index sensitivity, and inadequate detection capability for ultra-low concentration biomolecules in clinical settings. In this study, we developed a low-cost, high-sensitivity optical fiber SPR biosensor for SARS-CoV-2 antigen detection in the standard sample, employing gold@silver‑platinum (Au@AgPt) core-shell nanospheres as the sensing enhancement module. Au@AgPt core-shell nanospheres possess unique localized surface plasmon resonance (LSPR) properties. When incident light interacts with the nanomaterial surface, it induces a localized electric field enhancement effect. This surface enhancement effect significantly amplifies the optical signal intensity, thereby effectively improving the sensor's detection sensitivity toward the receptor-binding domain (RBD) of the SARS-CoV-2 spike protein. This sensing platform achieves fg/mL-level detection sensitivity, excellent linearity, and rapid response performance, offering new technical support for expanding the application of optical fiber sensors in the quantitative detection of ultra-low concentration disease markers.
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JOURNAL OF ELECTRONIC MATERIALS
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2026
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IF 2.8
Li Qiu,Zhang Tingting,Wang Yu,Li Xi,Nie Congcong,Lv Changpeng,Song Jingjing,Huang Qihao,Wu Fang
Ni(OH) 2 is considered as one of most promising oxygen evolution reaction (OER) catalysts owing to its low cost and earth abundance. Nevertheless, the low conductivity and poor intrinsic activity of Ni(OH) 2 seriously restricts its industrial application. Meanwhile, the relationship between the structure, OER activity of Ni(OH) 2 -based catalysts, and transition-metal dopants is still unclear. To meet above challenges, a series of Ni(OH) 2 –M (M = Fe, Al, Co, Cr) was prepared by a simple hydrothermal method. The experimental results confirmed that the incorporation of different metal elements into Ni(OH) 2 can not only alter the morphology and modulate electronic structure but also improve the intrinsic catalytic activity. Particularly, the Ni(OH) 2 –Fe exhibits the best OER activity, requiring 252 mV and 288 mV at 10 mA cm −2 and 100 mA cm −2 , with a Tafel slope of 39.1 mV dec −1 and superior stability (50 mA cm −2 for 30 h). This work provides a pathway to understand the relationship between structure, OER activity of Ni(OH) 2 -based catalysts, and incorporation of transition-metal elements, which facilitates the development of advanced OER electrocatalysts.
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