SENSORS AND ACTUATORS B-CHEMICAL
·
2011
·
IF 3.37
Qingxiang Wang,Bin Zhang,Xiaoqiang Lin,Wen Weng
A label-free DNA biosensor for hybridization detection of short DNA species related to the transgenic plants gene fragment of cauliflower mosaic virus (CaMV) 35S promoter was developed in this paper. The nanocomposite containing chitosan (CS) and mutiwalled carbon nanotubes (MWNTs) was first coated on a glassy carbon electrode. Then a highly reactive dialdehyde reagent of glutaraldehyde (GTD) was applied as an arm linker to covalently graft the 5′-amino modified probe DNA to the CS–MWNTs surface via the facile aldehyde–ammonia condensation reaction. The hybridization capacity of the developed biosensor was monitored with electrochemical impedance spectroscopy (EIS) using [Fe(CN) 6 ] 3−/4− as an indicating probe, and the experimental results showed that the biosensor had fast hybridization rate and low background interference. A wide dynamic detection range (1.0 × 10 −13 –5 × 10 −10 M) and a low detection limit (8.5 × 10 −14 M) were achieved for the complementary sequence. In addition, the hybridization specificity experiments showed that the sensing system can accurately discriminate complementary sequence from mismatch and noncomplementary sequences.
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ANALYTICAL AND BIOANALYTICAL CHEMISTRY
·
2020
·Vol. 412
·期 4
·915-922
·
IF 3.637
Wu Zhi-Wei,Xie Xing-Chen,Guo Hong-Ran,Xia Huan,Huang Ke-Jing
A tetrahedral DNA probe can effectively overcome the steric effects of a single-stranded probe to obtain well-controlled density and minimize nonspecific adsorption. Herein, a highly sensitive electrochemical biosensor is fabricated for determination of protein using a tetrahedral DNA probe and rolling circle amplification (RCA). N- and P-co-doped graphene (NP-rGO) is prepared, and AuNPs are then electrodeposited on it for DNA probe immobilization. Benefitting from the synergistic effects of the excellent electrical conductivity of NP-rGO, the stability of the tetrahedral DNA probe and the signal amplification of RCA, the biosensor achieves a low limit of 3.53 × 10 −14 M for thrombin and a wide linear range from 1 × 10 −13 to 1 × 10 −7 M. This study provides a sensitive and effective method for the detection of protein in peripheral biofluids, and paves the way for future clinical diagnostics and treatment of disease.
Graphical abstract
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NEW JOURNAL OF CHEMISTRY
·
2018
·Vol. 42
·期 7
·5178-5184
·
IF 3.201
Xingxing Zhang,Yan Jin,Baoxin Li
A copper nanoclusters (CuNCs)-based fluorescence biosensing platform was developed for label-free DNA detection through a cascade isothermal exponential amplification reaction (EXPAR). It is based on target-triggered isothermal dual-cycling and a cascade EXPAR, which generates a large amount of long double-stranded DNA (dsDNA). The resultant long dsDNA can act as an efficient template for fluorescent CuNC synthesis, and the produced CuNC is an excellent fluorescence indicator to quantify target DNA concentration. This assay allows the detection of the model DNA with a detection limit of 7 pM. This label-free assay occurs in a homogenous solution, and the whole assay process, including hybridization, amplification and detection, can be completed in a single-tube. This method is simple, low-cost and convenient to operate, and may offer potential applications in related biomedical research.
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INTERNATIONAL JOURNAL OF BIOLOGICAL MACROMOLECULES
·
2022
·
IF 8.025
Xiaoying Wang,Rong Chen,Juanjuan Hu,Wei Yuan
Amyloid-β oligomers (AβOs) was the core-biomarker of Alzheimer's disease (AD), and the detection of AβOs is very important for the early diagnosis of AD. However, existing tests for AβOs are majorly suffering from complex process and poor sensitivity. Thus, an adjustable AβOs electrochemiluminescence (ECL) aptasensor based on the synergistic effect of self-enhanced metal-organic gel (AgCNS) and triple-helix DNA system (THS) was successfully constructed. AgCNS was prepared by an extremely simple one-pot method and was an innovative luminophore with excellent ECL performance. The AgCNS-labeled complementary sequence ( [email protected] ) was interlaced with the unlabeled aptamer (Apt) carrying two short-arms fixed on the gold electrode (GE) to form the THS. Along with the specific-binding of AβOs and Apt, the THS was disrupted and adjusted flexibly between “on” and “off”, resulting in significant changes in the ECL signals. Thus, ECL detection of AβOs was sensitively achieved with a detection limit as low as 0.23 fM and the different forms of Aβ can be specifically distinguished. The aptasensor also exhibited satisfactory selectivity, stability and reproducibility. Moreover, when proposed method and ELISA-kit were simultaneously applied to artificial cerebrospinal fluid (A-CSF) samples, the obtained results were completely consistent, reflecting the potential clinical application value of this work.
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Journal of Biomedical Nanotechnology
·
2022
·Vol. 18
·期 6
·1585-1595
·
IF 3.641
Chen Ben-Qi,Yang Meng,Liao Wen-Chun,Li Zi-Ying,Chen Zhi-Xiong,Yang Yun-Hui,Yang Tong,Hu Rong
Developing a strategy for the determination of trace ochratoxin A (OTA) in food is very important to ensure food safety. Here, based on a tripod probe, an electrochemical aptasensing platform for detected OTA sensitively was designed, which used the 3D DNA walker by endonuclease Nb.BbvCI
powered and the nanozyme of porous Co3O4 nanodisks loaded Pt nanoparticles (Pt NPs@Co3O4) as a cascaded signal amplifying device. With OTA as a model target, the swing-arm probe of DNA walker was triggered. Next, probe on the magnetic beads was cleaved
and released by Nb.BbvCI to realize the first signal amplification. After that, the partial sequence of the released probe is well assembled with triple-strand DNA by strand reaction and the other sequence exposed as a capture probe was combined with the Pt NPs@Co3O4.
It can efficiently make the decomposition of H2O2, bring about significant current, and the signal was amplified again. The designed electrochemical aptasensor demonstrated outstanding detection linearity in the OTA concentration range of 1 pg/mL to 100 ng/mL, with a
detection limit of 0.194 pg/mL (S/N = 3), with excellent reproducibility and high specificity. It can be used to find OTA in food. This research supplies a potential split-new avenue for the sensitive detection of electrochemical aptasensor.
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JOURNAL OF AGRICULTURAL AND FOOD CHEMISTRY
·
2017
·
IF 3.154
Qian Luan,Ning Gan,Yuting Cao,Tianhua Li
A mimicking-enzyme-based colorimetric aptasensor was developed for the detection of kanamycin (KANA) in milk using magnetic loop-DNA-NMOF-Pt (m-L-DNA) probes and catalytic hairpin assembly (CHA)-assisted target recycling for signal amplification. The m-L-DNA probes were constructed via hybridization of hairpin DNA H1 (containing aptamer sequence) immobilized magnetic beads (m-H1) and signal DNA (sDNA, partial hybridization with H1) labeled nano Fe-MIL-88NH2-Pt (NMOF-Pt-sDNA). In the presence of KANA and complementary hairpin DNA H2, the m-L-DNA probes decomposed and formed an m-H1/KANA intermediate, which triggered the CHA reaction to form a stable duplex strand (m-H1-H2) while releasing KANA again for recycling. Consequently, numerous NMOF-Pt-sDNA as mimicking enzymes can synergistically catalyze 3,3′,5,5′-tetramethylbenzidine (TMB) for color development. The aptasensor exhibited high selectivity and sensitivity for KANA in milk with a detection limit of 0.2 pg mL–1 within 30 min. The assay can be conveniently extended for on-site screening of other antibiotics in foods by simply changing the base sequence of the probes.
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SENSORS AND ACTUATORS B-CHEMICAL
·
2025
·
IF 7.7
Linjing Duan,Huali Jin,Yang Wen,Min Wei,Rui Guo,Zhiguang Suo,Keshavan Niranjan
As a food and environmental contaminant having harmful qualities like neurotoxicity and carcinogenicity, acrylamide (AA) is a risk to human health. In this study, Ce-MOF@AuPd NFs were utilized as electrode modification materials to develop a signal-off electrochemical aptasensor in combination with Mg 2 + -dependent DNAzyme-driven 3D DNA walker and triplex molecular switch (THMS) for the sensitive detection of AA. Ce-MOF@AuPd NFs exhibit an expansive surface area and enhanced conductive properties, enabling the binding of substantial DNA sequences and speeding up electron transfer. The Mg 2+ -dependent DNAzyme-driven 3D DNA walker improves the nucleic acid shearing efficiency with excellent signal amplification performance, and combines with the DNA-specific structure THMS to further boost sensor sensitivity. The sensor also demonstrated excellent specificity, reproducibility, repeatability, and stability, along with achieving satisfactory recovery rates when tested on potato chips and coffee samples. The electrochemical aptasensor offers strong application prospects for AA detection in food, according to the results.
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RSC Advances
·
2021
·Vol. 11
·期 61
·38820-38828
·
IF 3.361
Yujie Sun,Zhenzhong Lu,Wenlin Ma,Rui Wang,Chengwu Zhang,Jinhua Liu
One key challenge in oligonucleotide sequence sensing is to achieve multiplexed DNA detection in one sensor. Herein, a simple and efficient fluorescent biosensing platform is constructed to simultaneously detect multiplexed DNA depending on porous organic polymer (POP) nanospheres. The developed sensor is based on the concept that the POP nanospheres can efficiently quench the fluorescence emission of dye-labeled single-stranded DNA (ssDNA). Fluorescence quenching is achieved by the non-covalent assembly of multiple probes on the surface of POP nanospheres through electrostatic attraction and π–π stacking interactions, in which the electrostatic attraction plays a more critical role than π–π stacking. The formed dsDNA could be released off the surface of POP via hybridizing with the target DNA. Consequently, the target DNA can be quickly detected by fluorescence recovery. The biosensor could sensitively and specifically identify three target DNAs in the range of 0.1 to 36 nM, and the lowest detection limits are 50 pM, 100 pM, and 50 pM, respectively. It is noteworthy that the proposed platform is successfully applied to detect DNA in human serum. We perceive that the proposed sensing system represents a simple and sensitive strategy towards simultaneous and multiplexed assays for DNA monitoring and early clinical diagnosis.
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SENSORS AND ACTUATORS B-CHEMICAL
·
2016
·
IF 4.758
Shulian Bai,Ting Wang,Zhang Zhang,Shangchun Sheng,Wen Yu,Guoming Xie
Adopting the concept and operation of hybridization-based associative toehold activation (HATA) concatemer induced catalyzed hairpin assembly (CHA), a novel colorimetric biosensor was developed for detecting target DNA and human alpha-thrombin. The assemble of separate DNA hairpin components can be triggered by specific target binding that are otherwise unable to spontaneously happen. In the presence of four ingeniously designed hairpin structures, the target molecule can trigger two cascade recycling reactions: HATA concatemer reaction and CHA reaction. In the HATA concatemer reaction, the toehold and branch migration domain overhang at the 5′ end and 3′ end of hairpin probe H1, respectively. Upon the addition of target molecule, plenty of toeholds and branch migration domains can be brought into close proximity via DNA self-assemble. Then, the toeholds and branch migration domains of HATA products serve as the trigger of CHA. In this concatemer recycling, numerous guanine rich DNA duplexes can be produced through the highly effective use of target molecule. The proposed sensing system exhibits a remarkable analytical performance and an ultrahigh discrimination capability for proximal mismatch. Under optimal conditions, the calibration curves have a good linearity from 1 pM to 75 nM and 1 pM to 2.5 nM for target DNA and human alpha-thrombin, respectively. The corresponding detection limits are 0.14 pMand 0.68 pM Therefore, this new biosensor has a great potential for further applications in biomedical research and demonstrates excellent versatility for different analytes.
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International Journal of Nanomedicine
·
2023
·
IF 8
Luchen Zhang,Zhenbo Li,Fangli Sun,Yuhong Xu,Zixiu Du
A safe and efficient quaternary gene delivery system (named Q-complexes) was constructed based on self-assembly of molecules through noncovalent bonds. This system was formulated through the cooperation and competing interactions of cationic liposomes, multifunctional peptides, and DNA, followed by coating hyaluronic acid on the surface of the ternary complexes. The multifunctional peptide was composed of two functional domains: penetrating hepatic tumor-targeted cell moiety (KRPTMRFRYTWNPMK) and a wrapping gene sequence (polyarginine 16). The effect of spacer insertion between the two domains of multifunctional peptide on the intracellular transfection of Q-complexes was further studied. Experimental results showed that the formulations assembled with various peptides in the spacer elements possessed different intercellular pathways and transfection efficiencies. The Q-complexes containing peptide in the absence of spacer element (Pa) showed the highest gene expression among all samples. The Q-complexes containing peptides with a noncleavable spacer GA (Pc) had no ability of intracellular nucleic acid delivery, whereas those with a cleavable spacer RVRR (Pd) showed moderate transfection activity. These results demonstrated that the different spacers inserted in the multifunctional peptide played an important role in in vitro DNA transfection efficiency. Atomic force microscopy images showed that the morphologies of ternary complexes (LPcD) and Q-complexes (HLcPD) were crystal lamellas, whereas those of other nanocomplexes were spheres. Circular dichroism showed the changed configuration of peptide with spacer GA in nanocomplexes compared with that of its free state, whereas the Pa configuration without spacer in nanocomplexes was consistent with that of its free state. The present study contributed to the structural understanding of Q-complexes, and further effective modification is in progress.
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BIOSENSORS & BIOELECTRONICS
·
2020
·
IF 10.257
Chengxi Zhu,Dong Liu,Yuye Li,Shuai Ma,Meng Wang,Tianyan You
The simultaneous detection of multiple mycotoxins in grains is significant due to the enhanced toxicity induced by their synergistic effects . In this work, a dual-ratiometric electrochemical aptasensing strategy for the simultaneous detection of aflatoxin B1 (AFB1) and ochratoxin A (OTA) was developed. Here, an anthraquinone-2-carboxylic acid (AQ)-labelled complementary DNA (cDNA) was used to provide separate and specific binding sites to assemble the ferrocene-labelled AFB1 aptamer (Fc-Apt1) and methylene blue-labelled OTA aptamer (MB-Apt2). The target-induced current ratios of I Fc /I AQ and I MB /I AQ were then used to quantitatively relate to AFB1 and OTA, respectively. Following this principle, two types of aptasensors involving the hairpin DNA (hDNA) and linear single-stranded DNA (ssDNA) as the cDNA were fabricated for performance comparisons. The results revealed that hairpin DNA with a rigid 2D structure can greatly improve the assembly and recognition efficiency of the sensing interface, which makes the hDNA-based aptasensor possess high sensitivity, reliability and anti-interference ability. The hDNA-based aptasensor exhibited a detection range of 10-3000 pg mL −1 for AFB1 and 30-10000 pg mL −1 for OTA, respectively, with no observable cross-reactivity. Furthermore, the aptasensor was applied to analyze corn and wheat samples, and the reliability was validated by HPLC-MS/MS. Our work has presented a novel way for fabricating a high-performance aptasensor for simultaneous detection of multiple mycotoxins .
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JOURNAL OF HAZARDOUS MATERIALS
·
2025
·
IF 11.3
Yudie Sun,Aobo Feng,Ruilai Zhang,Kehong Quan,Zefeng Hua,Kui Zhang
Bacterial contamination is a very serious health and environmental problem, with the main source of toxicity being lipopolysaccharides in the cell walls of Gram-negative bacteria. Therefore, the development of effective analytical methods is crucial for the detection of lipopolysaccharide content. This work facilitates the efficient generation of precisely adjustable dual-mode signals for LPS detection in surface-enhanced Raman spectroscopy (SERS) and electrochemiluminescence (ECL) by inducing anisotropic morphological evolution of Au@Ag nanocubes (Au@AgNCs) through poly-cytosine (poly-C) DNA. In the presence of LPS, the released probe triggers rolling circle amplification (RCA) and generates cyclic poly-C DNA, which subsequently induces the etching of Ag composites on Au@AgNCs. This etching process leads to changes in both the morphology and composition of Au@AgNCs composite materials, resulting in a decrease in SERS signals. The poly-C DNA was subsequently adsorbed with Ag + and then reduced to silver nanoclusters (AgNC) by NaBH 4 , serving as an ECL signal source. Under optimal experimental conditions, both SERS and ECL demonstrated linear ranges spanning from 1 fg/mL to 1 ng/mL, with detection limits of 0.29 fg/mL and 0.14 fg/mL, respectively. Moreover, the SERS-ECL dual-mode sensor facilitates the accurate discrimination of Gram-negative bacteria in diverse real samples. It demonstrates promising applications in the areas of food safety, water quality monitoring, and clinical bacterial detection.
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