PESTICIDE BIOCHEMISTRY AND PHYSIOLOGY
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2018
·
IF 3.44
Xuegui Wang,Xing Xiang,Huiling Yu,Shuhua Liu,Yong Yin,Peng Cui,Yaqiong Wu,Jing Yang,Chunxian Jiang,Qunfang Yang
The beet armyworm Spodoptera exigua , a major pest affecting numerous cultivated crops in China, has developed a serious resistance to many traditional chemical insecticides . The resistance levels of the field-collected populations from different districts in Sichuan Province, China, to nine insecticides were detected with a diet-incorporation bioassay. Compared to the Lab-ZN strain, five (in 2014) and three (in 2016) field populations displayed either high or extremely high levels of resistance to beta-cypermethrin. All the field populations collected in 2014 were susceptible to emamectin benzoate, hexaflumuron, methoxyfenozide, chlorantraniliprole, cyantraniliprole and indoxacarb but exhibited low or moderate levels of resistance to abamectin . The resistances of field populations collected in 2016 were significantly higher than two years earlier, especial for chlorantraniliprole and cyantraniliprole with RRs rising from 173.4- to 582.6-fold and 175.3- to 287.6-fold, respectively, even though the field populations had retained moderate or low levels of resistance to chlorpyrifos and hexaflumuron. The synergism experiment revealed that the resistance of the LS16 population to beta-cypermethrin may be mainly related to cytochrome P450 monooxygenases (P450s), which was responsible for the highest increase ratio of 37.97-fold, for piperonyl butoxide , rather than either carboxylesterase (CarE) or glutathione S-transferase (GST). The cytochrome P450 ethoxycoumarin O -deethylase activity of the LS16 population was also the strongest among the treatments ( P < 0.05). Non-denaturing polyacrylamide gel electrophoresis (native PAGE) indicated that enhanced E11, E13 and E15-E16 bands in the LS16 population likely contribute to the development of resistance to beta-cypermethrin.
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ACS ES&T Water
·
2024
·
IF 4.8
Yongyan Chen,Jia Lyu,Shenghua Gao,Bixiong Ye,Lan Zhang
In this study, the raw water, finished water, and tap water of various waterworks in major river basins of China were collected for the determination of the neonicotinoid insecticides (NNIs). The results showed that one or more NNIs were detected in 81.2% of the water samples, and the total concentration of NNIs (∑NNIs, sum of eight NNIs) ranged from 0.10 to 210.33 ng/L, with a median of 7.08 ng/L. Thiamethoxam, imidacloprid, and clothianidin had the highest contribution rate among the major ∑NNIs detected. The concentration of ∑NNIs detected in the Pearl River was higher than that in other river basins, with a median of 51.11 ng/L. During the dry season, the detection rate of ∑NNIs was 77.4%, with concentrations ranging from 0.10 to 210.33 ng/L. During the wet season, the detection rate of ∑NNIs was 85%, and the concentration of ∑NNIs ranged from 0.10 to 117.03 ng/L. The estimated daily intake (EDI) of NNIs in the raw water and drinking water of each river basin is less than the reference dose (RfD) of imidacloprid. The potential risk of NNIs can be reduced by further optimizing the water treatment processes.
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CHEMICAL ENGINEERING JOURNAL
·
2024
·
IF 13.3
Junxi Lei,Limin Deng,Zongda Chen,Xiang Li,Kaixin Li,Jong-Min Lee
Saccharification serves as the entry point for the biomass into biorefinery schemes and plays a vital role in lignocellulose transformation towards bio-fuels and platform molecules. Herein, an efficient in situ saccharification of lignocellulose under the mediation of ionic liquid of [DMIM]DMP is reported, using a series of magnetically-separable biochars as catalyst supports with immobilized endoglucanase Cel5A. The physicochemical properties of these magnetic biochars with immobilized endoglucanase (abbreviated as MBIE) were thoroughly characterized by FTIR, TG, SEM/EDX, XRD, XPS, N 2 physisorption, UV–vis and VSM. The results revealed that the endoglucanase, which functions as the catalytically active species, was immobilized on the biochar via simultaneous physical adsorption and covalent attachment. The extremely high surface area of biochar endows a high loading of endoglucanase and easy diffusion of reactant within the catalyst’s framework. The zeta potential of the catalyst was − 7.06 mV, indicating its excellent stability. MBIE with immobilizing endoglucanase of 484.36 mg/g exhibited the superior performance under a biphasic system (IL/water v/v 5:1), affording a glucose yield of 932.5 mg/g. The activity of MBIE remained constant after 10 consecutive cycles via magnetic recycling. This work demonstrates an efficient in situ saccharification process catalyzed by the developed MBIE with high activity and excellent regeneration ability.
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CHEMICAL ENGINEERING JOURNAL
·
2024
·
IF 13.3
Junyang Yin,Feng Wu,Jiangnan Dai,Changqing Chen
Perovskite quantum dots (PQDs) are emerging as promising nanomaterials for optoelectronic applications. However, developing a cost-effective green synthesis process that simultaneously possesses satisfactory photoluminescence quantum yield (PLQY) and stability remains a severe challenge. Here, CsPbBr 3 @PbSO 4 nanocomposites with a near-unity PLQY of 99.8 % and robust ambient stability are synthesized in situ in water via 4-Bromobenzenesulfonic acid and ditetradecylamine ligands. Notably, these nanocomposites show no decline in PLQY even after being immersed in water for over 250 days, and also exhibit superior stability against heating, ultrasonication, UV irradiation, anion exchange, and erosion by polar solvents. First-principles calculations reveal that CsPbBr 3 @PbSO 4 nanocomposites can form a natural quantum well structure, which effectively promotes the radiative recombination of carriers in CsPbBr 3 QDs. Meanwhile, the remarkable stability is attributed to the ultrahigh decomposition enthalpy of PbSO 4 that serves as a strong barrier preventing the invasion of anions and polar media into the embedded CsPbBr 3 QDs. Finally, besides the convenient application of green fluorescent paper, a fingerprint recognition is demonstrated by fabricating a bright-green LED integrated with the CsPbBr 3 @PbSO 4 nanocomposites powder and a commercial UV chip. This work provides a new strategy for green synthesis and practical application of high-efficient and ultrastable PQDs.
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APPLIED CLAY SCIENCE
·
2024
·
IF 5.3
Jixing Fan,Qian Liu,Li,Li,Guanzheng Zhuang,Peng Yuan
Maya Blue, a hybrid pigment that can be synthesized by heating a mixture of palygorskite (or sepiolite) and indigo, has unclear relationships between the structural features of clay substrates, heating atmosphere, colors, and stability. This study investigates the color properties, stability, and clay-dye interactions of Maya Blue pigments based on palygorskite and sepiolite, heated under air and vacuum conditions (180 °C for 8 h). Reflectance spectra, CIE color parameters, surface properties, and Fourier-transform infrared spectroscopy were employed to evaluate these pigments. Results indicate that indigo monomers can penetrate the channels of both palygorskite and sepiolite, conferring a greenish-blue hue and high stability. However, the diffusion of indigo is determined by the size match between the channel and indigo monomers. In palygorskite, indigo monomers can diffuse only upon heating because the channel size is only a little bit larger than the indigo molecule, while in sepiolite, lager channels allow easy diffusion, even without heating. However, sepiolite's larger channels make indigo molecules more prone to chemical degradation or migration. Under vacuum conditions, indigo molecules more easily diffuse into the channels of both clay minerals, yielding pigments with higher saturation and enhanced stability. These findings highlight the size-matching between clay minerals' micropores and organic molecules and offer valuable guidance for optimizing the preparation process of clay-dye hybrid pigments.
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SENSORS AND ACTUATORS B-CHEMICAL
·
2024
·
IF 8
Hong Yang,Shanshan Zhu,Lihua Pang,Yifei Ma,Xiaoxue Liu,Jinhua Liu
ABSTRACT Using a single emission-wavelength to accurately/sensitively quantify disease markers in complex environments still faces enormous challenges. In this study, we construct a Cu 2+ -triggered wavelength-tunable fluorescent immunoassay for real-time detection of cTnI in human serum. The developed platform is based on an “on-off-on” strategy, which involves the rapid in-situ fluorogenic reaction of dopamine (DA) and phenol analogue by Cu 2+ , strong coupling between PPi and Cu 2+ as well as the specific hydrolysis of PPi into orthophosphate by alkaline phosphatase. By using cTnI as a model antigen, the assay shows good linearity relationships between the fluorescence response and cTnI concentration. Furthermore, we used different phenol analogues (1,3-dihydroxynaphthalene, 8-hydroxyjulolidine, 1,5-dihydroxynaphthalene) as fluorescence reaction substrates to achieve multi-channel (blue, green and yellow) real-time monitoring of cTnI with detection limits of 0.17 ng/mL, 0.17 ng/mL and 0.33 ng/mL, respectively. We successfully applied the wavelength-tunable ELISA to detect cTnI levels in real human serum, and the results were compared with a commercial ELISA with satisfactory outcomes. Therefore, our proposed platform demonstrates great potential in the early diagnosis of acute myocardial infarction.
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RUSSIAN JOURNAL OF BIOORGANIC CHEMISTRY
·
2025
·Vol. 51
·期 1
·137-144
·
IF 1.7
Mironova A. G.,Afanasyeva S. I.,Sybachin A. V.,Spiridonov V. V.,Bolshakov M. A.,Simonenko E. Yu.
Objective: Cholesterol is an important structural component of the plasma membrane of mammalian cells. Cholesterol, among other important roles, plays a special role in sperm membranes. Change in the lipid composition of the sperm membrane, particularly the outflow of cholesterol, is an integral part of the process of capacitation and subsequent acrosomal reaction necessary for the sperm to fertilize an egg. Deviations in cholesterol concentration in sperm membrane may indicate a decrease in the fertilizing potential of sperm. To determine the optimal method for rapid analysis of the cholesterol content in human sperm membranes in the IVF laboratory, four methods of quantitative determination of cholesterol were compared in terms of practicality and effectiveness of their use to assess the concentration of cholesterol in human sperm membranes: the method of enzymatic colorimetric detection, the Lieberman–Burchard method, infrared spectroscopy and high-performance liquid chromatography. Methods: 101 ejaculates of patients with established normozoospermia (according to WHO criteria) were used in the work. Spermatozoa were separated from the semen by double centrifugation with the addition of DPBS medium. The resulting cellular pellet was used to determine the concentration of cholesterol in a sample by one of four methods: enzymatic colorimetric detection (FCD), HPLC, Lieberman-Burchard method, infrared spectroscopy. Results and Discussion: The following cholesterol concentrations were obtained by enzymatic colorimetric detection, Lieberman–Burchard, infrared spectroscopy and high-performance liquid chromatography: 1.0 ± 0.3, 1.32 ± 0.15, 5.1 ± 1.8 and 1.53 ± 0.18 nmol/10 6 cells, respectively. The Lieberman–Burchard method, enzymatic colorimetric detection and HPLC showed similar results, the obtained average cholesterol concentrations coincide within the error. The mean cholesterol concentration in sperm membranes obtained using infrared spectroscopy method significantly exceeds the values presented in the literature and the values obtained using other methods. In addition, this method requires an amount of analyzed material that significantly exceeds the volume of one ejaculate. Conclusions: As a result of comparing four methods of quantitative cholesterol analysis, the method of enzymatic colorimetric detection is proposed as a method of rapid analysis of cholesterol in human sperm membranes suitable for routine use in a clinical laboratory. The advantages of this method include the low toxicity of the method, it’s cost-effectiveness and a significant reduction in the time of complete analysis: from sample preparation to obtaining the result.
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JOURNAL OF AGRICULTURAL AND FOOD CHEMISTRY
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2024
·
IF 5.7
Yongmei Chang,Weihuan Xu,Shan Wang,Mengyao Zhu,Yanan Ru,Zhiheng Xu,Guangyou Chen,Yongqiang Li
Helicoverpa armigera Hübner (Lepidoptera: Noctuidae) is a globally devastating pest and has evolved with varying levels of resistance to a broader spectrum of insecticides. CarEs are major enzymes involved in insecticide detoxification and metabolic resistance. Four CarE genes were identified and cloned from H. armigera, and the expression pattern analyses indicated that they were predominantly expressed in the detoxifying tissues and larval feeding periods. The insecticide inductive assays further suggested that these CarE genes with expressions were significantly upregulated after beta-cypermethrin, lambda-cyhalothrin, malathion, and chlorpyrifos exposures. The purified recombinant proteins of both CarE016B and CarE016C by bacterial expression exhibited significantly higher catalytic efficiency toward α-naphthyl acetate and also showed relatively stronger binding with both β-cypermethrin and λ-cyhalothrin compared to the CarE006C and CarE015A. In vitro metabolism assay with HPLC suggests that CarE016B and CarE016C have the ability to metabolize β-cypermethrin, λ-cyhalothrin, and malathion with varying hydrolase activities. GC-MS/MS analysis identified 3-phenoxybenzaldehyde (3-PBAld) as the metabolite of both β-cypermethrin and λ-cyhalothrin. Homology modeling and molecular docking analyses further indicate that these three types of insecticides could be anchored into the active pocket of both CarEs. Collectively, these results demonstrate that both CarE016B and CarE016C play a critical role in the detoxification of pyrethroid and organophosphate insecticides in H. armigera. This study provides the foundations for a comprehensive understanding of the role of the CarEs family in insecticide detoxification and resistance in H. armigera.
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Nature Communications
·
2024
·Vol. 15
·期 1
·1-10
·
IF 14.7
Li Min,Li Hong,Fan Hefei,Liu Qianfeng,Yan Zhao,Wang Aiqin,Yang Bing,Wang Erdong
Hydrogen production from seawater remains challenging due to the deactivation of the hydrogen evolution reaction (HER) electrode under high current density. To overcome the activity-stability trade-offs in transition-metal sulfides, we propose a strategy to engineer sulfur migration by constructing a nickel-cobalt sulfides heterostructure with nitrogen-doped carbon shell encapsulation (CN@NiCoS) electrocatalyst. State-of-the-art ex situ / in situ characterizations and density functional theory calculations reveal the restructuring of the CN@NiCoS interface, clearly identifying dynamic sulfur migration. The NiCoS heterostructure stimulates sulfur migration by creating sulfur vacancies at the Ni 3 S 2 -Co 9 S 8 heterointerface, while the migrated sulfur atoms are subsequently captured by the CN shell via strong C-S bond, preventing sulfide dissolution into alkaline electrolyte. Remarkably, the dynamically formed sulfur-doped CN shell and sulfur vacancies pairing sites significantly enhances HER activity by altering the d -band center near Fermi level, resulting in a low overpotential of 4.6 and 8 mV at 10 mA cm −2 in alkaline freshwater and seawater media, and long-term stability up to 1000 h. This work thus provides a guidance for the design of high-performance HER electrocatalyst by engineering interfacial atomic migration.
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ACS Applied Materials & Interfaces
·
2024
·
IF 8.3
Yong Liu,Kaiyuan Wang,Wenwen Ren,Nannan Gao,Juanjuan Li,Hao Wang
Hydrogel-based dressing materials offer significant potential in expediting skin wound healing. Nevertheless, they face several challenges: poor adhesion to wound tissues, difficulties in preservation under ambient conditions, and limited multifunctionality to support all wound healing stages. In this work, a dry patch is designed to address these persistent issues by featuring an in situ solid-to-gel transformation and Janus wet tissue adhesiveness. The HGP patch integrates a wet adhesive layer combining dopamine-conjugated hyaluronic acid (HD) and poly(acrylic acid) (PAA), a drug-loading layer comprising gelatin (Gel), and a nonadhesive gelation layer of poly(vinyl alcohol) (PVA) and sodium alginate (SA). This hierarchical structural design confers exceptional wound adhesion, hemostatic capabilities, and antibacterial and antioxidant activities, as well as immune regulatory properties. These attributes collectively support accelerated skin wound healing, particularly in cases complicated by bacterial infections. This research charts an approach to engineer hydrogel-based wound dressings through on-site hydrogel formation, thus advancing the treatment of wounds afflicted with complex infections.
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ChemSusChem
·
2024
·
IF 7.5
Akkammagari Putta Rangapppa,Wenhua Xue,Anirban Chowdhury,Yixuan Liu,Jong-Min Lee,Jun Zhao
The photocatalytic conversion of biomass-based platform molecules, such as 5-hydroxymethylfurfural (HMF), holds significant importance for the utilization of biomass resources. This study focuses on the unique ability of atomically bridged indium (In) atoms that encourages inactive SnS2 surface and steer the selective HMF oxidation process under solar light. Experimental results suggest that In confined SnS2 structure provides not only favorable sites and electronic structures for the synergistic activation of HMF/O2 but also benefit in charge carrier dynamics, thus influencing the overall activity and selectivity of the SnS2 catalyst. In addition, in-situ spectroscopy and density functional theory (DFT) analysis uncovered the multifunctional role of In sites in promoting the key steps of the catalytic process, from ROS generation to regulated adsorption/activation of *HMF which serves as the rate limiting step of the overall HMF oxidation process. Consequently, the optimized In-SnS2-0.75 photocatalyst demonstrated excellent photo oxidation performance, reaching a high HMF conversion efficiency, yield, and selectivity of 91.2, 73 and 80% respectively, in just two hours of the reaction. This study highlights the strategic approach of rationally designed catalytic systems in order to tune the ROS and the product distribution of the HMF oxidation process.
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Nano Energy
·
2025
·
IF 17.1
Wenpei Zhao,Qiuyu Liu,Ziyang Tian,Daqing Ma,Wenrui Li,Shuhong Wang,Yuting Xie,Jingqiao Zheng,Huiyuan Huang,Xiya Yang,Yantao Shi,Bing Yin,Yudi Wang
CsPbBr 3 perovskite has become a star material for triboelectric nanogenerators (TENGs) due to its excellent optoelectronic properties, dielectric properties and stability in the atmosphere. However, regulating its optoelectronic and dielectric properties through charge-directed transport and separation within TENGs has not been comprehensively explored. Herein, p-n junction is constructed to form built-in potential ( E built-in ) for charge-directed transport by introducing a semiconductor layer beneath the perovskite film in perovskite-TENGs, where the effect of E built-in ’s direction and intensity on output performance is systematically investigated. Introducing the p-type semiconductor NiO x creates a reversed E built-in , which enhances the surface triboelectric charge density of the perovskite and achieves improved triboelectric output performance of 205 V and 43 μA cm −2 for the PVDF-CsPbBr 3 TENG. Conversely, the n-type semiconductor SnO 2 establishes a parallel E built-in , greatly facilitating the extraction and separation of photogenerated carriers, leading to a high current density of 1.4 mA cm −2 under illumination based on the triboelectric-photoelectric coupling effect. Moreover, the CsPbBr 3 -TENGs demonstrate superior stability and a broad linear response range and fast photoresponsivity, proving their potential for practical applications in various self-powered optoelectronic detection devices.
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