MICROCHIMICA ACTA
·
2024
·Vol. 191
·期 8
·1-10
·
IF 5.3
Wang Panxue,Liu Ying,Li Xiang,Li Cen,Li Guoliang
A ratiometric SERS aptasensor based on catalytic hairpin self-assembly (CHA) mediated cyclic signal amplification strategy was developed for the rapid and reliable determination of Escherichia coli O157:H7. The recognition probe was synthesized by modifying magnetic beads with blocked aptamers, and the SERS probe was constructed by functionalizing gold nanoparticles (Au NPs) with hairpin structured DNA and 4-mercaptobenzonitrile (4-MBN). The recognition probe captured E. coli O157:H7 specifically and released the blocker DNA, which activated the CHA reaction on the SERS probe and turned on the SERS signal of 6-carboxyl-x-rhodamine (ROX). Meanwhile, 4-MBN was used as an internal reference to calibrate the matrix interference. Thus, sensitive and reliable determination and quantification of E. coli O157:H7 was established using the ratio of the SERS signal intensities of ROX to 4-MBN. This aptasensor enabled detection of 2.44 × 10 2 CFU/mL of E. coli O157:H7 in approximately 3 h without pre-culture and DNA extraction. In addition, good reliability and excellent reproducibility were observed for the determination of E. coli O157:H7 in spiked water and milk samples. This study offered a new solution for the design of rapid, sensitive, and reliable SERS aptasensors. Graphical abstract
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SENSORS AND ACTUATORS B-CHEMICAL
·
2024
·
IF 8
Yilin Zhao,Amin Zhang,Shuyun Feng,Tiantian Liu,Jingjing Ning,Yucai Zhang,Jin Cheng,Qian Zhang,Chunxia Wang
Sepsis is a critical condition characterized by high morbidity and mortality. The development of technologies for early recognition of sepsis is essential for enhancing patient outcomes. Our original finding indicates that ATP-citrate lyase (ACLY) holds promise as a diagnostic biomarker for sepsis; however, sensitive methods for detecting trace levels of ACLY in serum are currently lacking. We have therefore developed a novel AP-M@Anti ACLY detection probe based on surface-enhanced Raman scattering (SERS) for the detection of serum ACLY. The AuNBPs@MBA was synthesized using gold nanobipyramids (AuNBPs) as the base and 4-mercaptobenzoic acid (4-MBA) as a reflective indicator for ACLY activity. Specifically, the Raman signal of 4-MBA decreases proportionally with an increase in ACLY concentration. ACLY antibodies were subsequently linked to AuNBPs@MBA via the gold-sulfur bonds and blocked with bovine serum albumin (BSA). Under optimized conditions, the probe exhibits a strong linear correlation between Raman signal and ACLY concentrations, ranging from 1 pg/mL - 0.5 ng/mL (R 2 = 0.998) and 0.1–500 ng/mL (R 2 = 0.999), with a remarkable limit of detection (LOD) of 1.0 pg/mL. AP-M@Anti ACLY detection probe surpasses enzyme-linked immunosorbent assay (ELISA) when testing septic children's serum samples in terms of its detection range, accuracy, and analysis time. Recovery rates ranged from 96.80 % to 102.52 %. In conclusion, all results suggest that AP-M@Anti ACLY detection probe is a promising tool for the early diagnosis of sepsis and offers new insights into the development of preclinical approaches.
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SPECTROCHIMICA ACTA PART A-MOLECULAR AND BIOMOLECULAR SPECTROSCOPY
·
2025
·
IF 4.6
Yao Liu,Runzi Zhang,Xiaoyu Yang,Yi He,Xiao Yang
With the increasing concern for ecological environmental and food safety, the development of synergistic systems integrating efficient bisphenol trace sensing and green photocatalytic degradation has emerged as a current research focus. In this study, a novel surface-enhanced Raman scattering (SERS) sensing-degradation integrated platform was successfully developed for the detection and degradation of bisphenol through the uniform modification of hydrogen-bonded organic framework nanorods loaded with gold nanoparticles (HOFs@Au). Based on the remarkable molecular enrichment effect of the porous structure of HOFs and the strong localized surface plasmon resonance (LSPR) effect from the AuNPs, the composite system exhibited excellent trace detection performance. Experimental results revealed that the HOFs@Au not only enabled trace SERS detection of bisphenol A (BPA), bisphenol S (BPS), and bisphenol F (BPF), but also demonstrated excellent photocatalytic degradation performance. Based on the advantages of HOFs@Au, the SERS sensor demonstrated a detection limit as low as 10 −8 M, a high enhancement factor (EF) of 2.3 × 10 5 and photodegradation removal efficiency as high as 80 %, providing a novel solution for precise monitoring and green remediation of bisphenol contaminants. The system shows great potential for applications in ecological environmental risk early warning and food safety monitoring.
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ANALYST
·
2025
·
IF 3.3
Qing Xiang,Hao Wang,Shengdong Liu,Yilong Zheng,Shipan Wang,Huanhuan Zhang,Yonggang Min,Yuguang Ma
Acute myocardial infarction (AMI) is a severe cardiovascular disease, for which early diagnosis is critical for reducing mortality and improving patient outcomes. Cardiac troponin I (cTnI) is widely recognized as the “gold standard” biomarker for AMI due to its high specificity and sensitivity. The concentration of cTnI correlates directly with different stages of AMI. Therefore, the accurate detection of cTnI concentration is of paramount importance. However, the low concentration of cTnI in biological fluids requires ultrasensitive detection methods. In this study, we developed a sandwiched surface enhanced Raman scattering (SERS)-based biosensor composed of SERS-immune substrate, target antigen, and SERS nanotags and realized sensitive and accurate detection of cTnI. The SERS-immune substrate features an ordered, multi-tipped monolayer of Au nanostars fabricated using a three-phase interfacial self-assembly method and 4-(2-hydroxyerhyl)piperazine-1-erhanesulfonic acid (HEPES) buffer modification. Compared to Au nanosphere SERS substrates, the Au nanostar SERS substrates exhibited about a 3-fold increase in Raman enhancement and demonstrated good uniformity and batch stability. This novel SERS detection platform, leveraging dual plasmonic enhancement from both the SERS-immune substrate and SERS nanotags, achieves detection of cTnI with a limit of detection (LOD) as low as 9.09 pg/mL and a relative standard deviation (RSD) as low as 11.24%. Thus, the Au nanostar SERS substrates developed in this study demonstrate significant potential for rapid and accurate detection of cTnI.
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TALANTA
·
2021
·
IF 6.057
Chengcheng Huo,Wanying Han,Wei Tang,Xinrui Duan
The study of the interaction between small molecules and proteins is important. Surface-enhanced Raman spectroscopy (SERS) is suitable for such applications since it has the power of detecting a molecule based on its intrinsic nature and without labeling. Herein, the [email protected] SERS substrate supporting highly reflective metal liquid-like films (MeLLFs) with polyacrylamide hydrogels (PAAG) has high-density “hot spots” to provide excellent SERS activity. The [email protected] formed by AgNPs only has less than 15% SERS activity loss when stored in the air for more than three weeks. By using rhodamine 6G (R6G) as a model analyte , the AgNPs based [email protected] SERS substrate exhibits an enhancement factor (EF) as high as 8.0 × 10 6 , a limit of detection (LOD) of 76.8 pM (S/N = 3). Also, the formed PAAG provided a 3D molecular network to orderly secure the assembled nanoparticles (NPs), which not only improves the stability of NPs but also shields the Raman signal of proteins as high as 45 g/L allowing the direct determination of the binding rate of human serum albumin (HSA) and doxorubicin (DOX). A binding rate of about 70% was detected, which is consistent with previous reports. Thus, proposed the [email protected] SERS substrate can be used as a promising candidate for SERS measurement in complex biological samples.
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RSC Advances
·
2019
·Vol. 9
·期 5
·2857-2864
·
IF 3.049
Chenghua Zong,Mengyi Ge,Hong Pan,Jing Wang,Xinming Nie,Qingquan Zhang,Wenfeng Zhao,Xiaojun Liu,Yang Yu
Surface-enhanced Raman spectroscopy (SERS) has been one of the most promising analytical tools. Despite many efforts in the design of SERS substrates, it remains a great challenge for creating a general flexible substrate that could in situ detect analytes on diverse objects. Herein, we report our attempt to address this issue by developing a facile and versatile method capable of generating silver/gold nanoparticles in situ on the surface of a cellulose acetate (CA) polymer in a simple, cheap, practical, and capping agent-free way. The as-prepared substrates exhibit excellent sensitivity, which enabled detection of Rhodamine 6G at concentrations as low as 10−12 M. Taking advantage of the excellent flexibility and optical transparency of the CA matrix, the highly SERS-active substrate was applied for in situ identification and detection of pesticide residues on fruits. The results indicated that tetramethylthiuram disulfide (TMTD) and thiabendazole (TBZ) can be clearly identified at concentrations as low as 18.05 ng cm−2 and 15.1 ng cm−2, respectively, which were much lower than the maximum permitted residue doses with respect to food safety.
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NEW JOURNAL OF CHEMISTRY
·
2018
·Vol. 42
·期 14
·11660-11664
·
IF 3.201
Cheng Xu,Ding Cao,Wenya Lu,Jun Sun,Si Cheng
Pollutants in the real environment are mostly oil/water mixtures, therefore the oil phase and the water phase may lead to mutual interference when SERS detection is performed. The separation of pollutants from oil/water mixtures and the independent detection of the oil phase and the water phase have become emerging issues in real applications. However, it is still a challenge to detect highly diluted molecules in aqueous solution since the molecules in aqueous solution are usually far from the plasmonic sensitive surface due to the highly dispersed molecules in solution. Here, a superhydrophobic–superoleophilic plasmonic membrane for integrated oil/water separation and enhanced SERS detection is prepared. The as-fabricated plasmonic membrane can not only separate analytes in the water phase from an oil/water mixture but can also enrich analytes in the water phase into a very small area on the surface of the plasmonic membrane, where the hot spots are located. Superior SERS detection sensitivity for low concentrations of R6G (10−14 M) in an oil/water mixture was achieved, whereas the Raman signals of R6G (10−9 M) in an oil/water mixture on a traditional Ag nanoparticle-coated silica substrate were difficultly distinguished owing to the interference of the oil phase.
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NANOTECHNOLOGY
·
2019
·Vol. 30
·期 25
·255503
·
IF 3.399
Lang Li,Suxiao Deng,Hong Wang,Ruohu Zhang,Kai Zhu,Yang Lu,Zhile Wang,Shenfei Zong,Zhuyuan Wang,Yiping Cui
Remote sensing remains a challenge due to its demand for high sensitivity, convenient sampling and rapid response time. Surface enhanced Raman scattering (SERS) spectroscopy is a powerful analytical method for the detection of various samples. Here, aiming at increasing the sensitivity, a novel strategy for the preparation of a SERS probe is demonstrated by using hollow optical fiber tips decorated by layer-by-layer assembly of two kinds of nanoparticles. Specifically, Au@Ag core–shell nanorods and Ag nanospheres with opposite surface charge were assembled layer-by-layer on the tip of hollow optical fibers through electrostatic interaction. Then, much more hotspots are generated due to the close gap between the nanorods and nanospheres in the resultant 3D structure, which can lead to a dramatically enhanced SERS activity of the probe compared with that fabricated by pure silver sphere nanoparticles or nanorods. On the other hand, taking the advantages of the vibration spectroscopic fingerprints property of SERS spectra and the long-distance communication capacity of optical fibers, the remote online detection of biological species including proteins, funguses and cells can be easily achieved within a few minutes. Therefore, such a novel kind of optical fiber-SERS sensor holds great potential for the rapid detection of a wide range of samples due to its superiority of simplicity and high sensitivity.
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IEEE Photonics Journal
·
2024
·Vol. 16
·期 4
·1-9
·
IF 2.1
Wenying Zhou,Xue Han,Yanjun Wu,Guochao Shi,Shiqi Xu,Mingli Wang,Wenzhi Yuan,Jiahao Cui,Zelong Li
An effective SERS-based detection method assisted by machine learning algorithm has been developed to intelligently identify the pharmacodynamic substances in traditional Chinese medicine (TCM) which aided in the quality control and identification of TCM. In this work, the ultrasensitive grating-like core-shell Ag@SiO2@Cu@moth wing (Ag@SiO2@Cu@MW) SERS platform was explored which can precisely control the intergap distances by adjusting the thickness of the SiO2 layer. This SERS platform demonstrated reproducibility and reliability, with a low relative standard deviation (RSD) of 7.38% and an enhancement factor (EF) of 2.49 × 107. The optimized Ag30@SiO2(4)@Cu20@MW substrate were then applied in the accurately analyzing pharmacodynamic substances. Specifically, the label-free SERS analysis showed the distinct spectral features for Jujuboside A, Saikosaponin A and Timosaponin A-III. Machine learning algorithms, such as principal component analysis (PCA), decision tree (DT), support vector machine (SVM), k-nearest neighbors (kNN) were employed and further in differentiating with the three pharmacodynamic substances Raman spectrum groups. These results indicate that SERS technology in combination with machine learning algorithms can not only achieve rapid and accurate detection of different types of pharmacodynamic substances, but also promote the modernization and international application of TCM.
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ANALYTICAL LETTERS
·
2024
·
IF 1.6
Xinxing Jiang,Jihong Fu,Dandan Li,Wenxia Xue,Yaxue Zhang
An effective and sensitive SERS substrate (TPDH-COF/Au NPs) on a covalent organic framework (TPDH-COF) decorated with gold nanoparticles (Au NPs) was fabricated via electrostatic self-assembly and successfully applied for the determination of thiabendazole (TBZ) in fruit juice. The “raspberry-like” structure SERS substrate combined the properties of the abundant “hot spots” between the Au NPs and the adsorption performance of TPDH-COFs for trace analytes. Excellent SERS performance was observed, which achieved a high enhancement factor value of 2.01 × 108 for rhodamine 6 G, with considerable reproducibility and excellent long-term stability of at least 28 d. The limit of detection was estimated to be 5 ppb for TBZ in fruit juice. The result demonstrated that the TPDH-COF/Au NPs have great potential for SERS analyses and determination of pesticide residues in foods.
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ACS Sustainable Chemistry & Engineering
·
2024
·
IF 7.1
Jiaxin Liu,Mingdi Pan,Jiuzeng Jin,Yunshu Lu,Zhongmin Feng,Ting Sun
17β-E2 was the most active sterol estrogen and was widely used in animal husbandry for animal fattening and increasing milk production. Its abuse has resulted in residues in the environment. However, even very low concentrations could still enter the human body through the enrichment of the food chain and have adverse effects on the endocrine system. The SERS analysis method was employed in this work for the trace detection of 17β-E2 in environmental water. Cheap Ag NPs were selected as SERS sources. MoS2 provided a platform for the orderly arrangement of Ag NPs while making the material self-cleaning. rGO was coated in the outermost layer and had a protective effect on Ag NPs and could avoid oxidation during storage and use. At the same time, the target molecule could be adsorbed to reduce the distance between itself and Ag NPs, thus enhancing the SERS signal. The material had a high SERS enhancement factor (EF = 1.95 × 107), low detection limit (LOD = 5 × 10–12 M), good linear relationship (R2 = 0.994), good stability (RSD = 4.74%), and strong anti-interference ability. The recovery of 17β-E2 in farm wastewater samples was 100.15–108.00% and RSDs were 1.09–2.57%.
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ANALYTICA CHIMICA ACTA
·
2026
·
IF 6.1
Mengyue Wang,Jinyang Wu,Chengcheng Wang,Dongyu Liu,Ying Wang,Peng Zhao
Ischemic stroke (IS) demands ultra-early diagnosis to improve outcomes, yet conventional imaging modalities often fail to capture the critical window for intervention due to inherent temporal delays. Here, we report a surface-enhanced Raman scattering (SERS)-based plasmonic nanosensor that leverages gold hexagonal nanoparticles (GHNPs) as high-efficiency plasmonic amplifiers to enable simultaneous quantification of two key IS biomarkers-S100β and brain natriuretic peptide (BNP)-directly in whole blood. By engineering a sandwich immunoassay on a silver nanoparticle-modified conductive substrate, the GHNPs generate dense plasmonic “hotspots” that substantially amplify Raman signals, allowing for sensitive and stable detection without sample pretreatment. The sensor was validated in a rat middle cerebral artery occlusion (MCAO) model, capturing dynamic biomarker elevations within hours post-ischemia, and in clinical blood samples from IS patients, where biomarker levels were significantly elevated compared to healthy controls. The platform achieved detection limits as low as 7.18 fg/mL for S100β and 8.98 fg/mL for BNP, markedly outperforming conventional ELISA. By enabling rapid, multiplexed, and highly accurate quantification of these two complementary biomarkers, our SERS platform directly addresses the diagnostic gap between biochemical onset and clinical imaging, offering a powerful tool for ultra-early IS diagnosis and clinical stratification with strong potential for translational application.
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