MICROCHIMICA ACTA
·
2013
·Vol. 180
·期 7
·573-580
·
IF 3.434
Chen Lijian,Wu Na,Sun Bing,Su Haichao,Ai Shiyun
We describe a rapid and convenient colorimetric method for the detection of oxidative DNA damage caused by peroxynitrite (ONOO − ) using unmodified gold nanoparticles (AuNPs). AuNPs are stable in the presence of single-stranded DNA (ssDNA) against the aggregation induced by a high ionic strength. If adsorbed ssDNA are cleaved by ONOO − to form smaller fragments, the AuNPs rapidly aggregate due to electrostatic attraction. As a result, the color of the solution changes from red to blue, and this can be seen with bare eyes. We also have evaluated the activity of the antioxidants gallic acid, ascorbic acid and caffeic acid to scavenge ONOO − . This method therefore also can be applied to screen for anti-oxidation drugs and agents.
ONOO − -induced ssDNA cleavage can be visually detected by a red-to-blue color change of AuNPs.
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MICROCHIMICA ACTA
·
2016
·Vol. 184
·期 2
·541-546
·
IF 4.831
Zhang Ruoxi,Deng Li,Zhu Paijin,Xu Shuxia,Huang Chengpeng,Zeng Ying,Ni Shijun,Zhang Xinfeng
The article describes a bienzyme visual system for aptamer-based assay of Hg(II) at nanomolar levels. The detection scheme is based on the finding that Hg(II) ions captured by aptamer-functionalized magnetic beads are capable of inhibiting the enzymatic activity of uricase and thus affect the formation of H 2 O 2 and the blue product, i.e., oxidized tetramethylbenzidine. This strategy allows for a visual detection of Hg(II) at nanomolar levels without additional amplification procedure. Measuring the absorbance at 650 nm, the logarithmic calibration plot is linear in the concentration range of 0.5–50 nM and the limit of detection (LOD) is 0.15 nM. This is as low as the LOD obtained by atomic fluorescence spectrometry (AFS). The ions K + , Mg 2+ , Na + , Ca 2+ , Cu 2+ , Zn 2+ , Fe 3+ , Al 3+ , Co 2+ , AsO 2
− , Ni 2+ , Cd 2+ and Pb 2+ do not have a significant effect on color formation. The method was applied to the analysis of (spiked) river water, lake water, mineral water, tap water and certified reference water samples, and the results agreed well with those obtained by AFS or certified values, with recoveries ranging from 97% to 109%. The relative standard deviation for five parallel detections at a 10 nM Hg(II) level is 5.2%.
A bienzyme-based visual aptasensor was fabricated for label-free detection of nanomolar Hg 2+ in water samples without any amplification or enrichment procedure.
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ANALYTICAL AND BIOANALYTICAL CHEMISTRY
·
2018
·Vol. 411
·期 19
·4569-4576
·
IF 3.307
Wang Kun,Zhai Fu-Heng,He Meng-Qi,Wang Jin,Yu Yong-Liang,He Rong-Huan
A simple fluorescence biosensor is developed based on the enzyme-assisted cascade amplification strategy. The amplification system consists of a hairpin-structure DNA (H-DNA) and exonuclease III. The target DNA can hybridize with the H-DNA and initiate exonuclease III-assisted target recycling amplification to generate abundant G-rich DNA (G-DNA). One region of G-DNA is designed to possess the same sequence as target DNA. Thus, the G-DNA can also hybridize with H-DNA and initiate the digestion of H-DNA. The cascade strategy in this amplification system causes the concentration of G-DNA to grow exponentially. The fluorescence intensity of N -methylmesoporphyrin IX (NMM) is highly enhanced due to the formation of G-quadruplex configuration. Under optimal conditions, the cascade system could achieve an admirable sensitivity with a detection limit of 52 fM for HIV DNA, and guarantees a satisfactory specificity. Moreover, the cascade system could be implemented for other target DNA detections by substituting the recognition region of the H-DNA. In this way, a detection limit of 65 fM for HBV DNA could be achieved by the cascade system. The target DNA analysis in a real serum sample further indicates that this biosensor has potential for future application in clinical diagnosis.
A simple and label-free cascade amplification strategy is developed by exploiting hairpin DNA and EXO III for sensitive DNA detection.
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JOURNAL OF MATERIALS SCIENCE-MATERIALS IN ELECTRONICS
·
2025
·Vol. 36
·期 6
·363
·
IF 2.8
Zhang Yi,Gu Yaohang,Wang Shuo,Zhang Xiaoyan,Qi Xiwei
Lead-free relaxor ferroelectrics (RFEs) have great potential applications in dielectric ceramic capacitors due to their distinguished energy storage performance, such as power pulse devices, manufacturing motors, sensors, and more. However, achieving high energy density and high efficiency simultaneously is a major challenge for practical applications. The performance of a capacitor depends largely on the interface between metal electrode and ceramics, which is related to the transfer of charge carrier process. In this work, the relaxation degree and defect dipole are manipulated by entropy manipulation and cation defect, while the surface micro-region area defect control is caused by the surface buried firing calcination process. We have designed and synthesized the performances of all the series of BaTiO 3 -basedperovskite ceramics as well as surface cation defect modification such as BaTiO 3 , Ba 0.95 TiO 3 , (Ba 0.95 Sr 0.05 )TiO 3 , (Ba 0.95-3x/2 Sr 0.05 Bi x )TiO 3 , resurfaced (Ba 0.95 Sr 0.05 )TiO 3 , and resurfaced (Ba 0.95-3x/2 Sr 0.05 Bi x )TiO 3 . Surface micro-region lattice distortions caused by the surface cation-defects reduce the carrier diffusion between the metal electrode and the BaTiO 3 -basedperovskite ceramic samples, which diminishes the polarization hysteresis and improving the energy storage efficiency. Specifically, the surface reconstructed (Ba 0.8 Sr 0.05 Bi 0.1 )TiO 3 ceramics exhibited excellent breakdown field strength characteristics ( E b = 155 kV·cm −1 ) and minimal hysteresis residual polarization characteristics ( P r = 1.9 μC ·cm −2 ), resulting in the largest storage density ( W rec = 1.193 J/cm 3 ) and highest efficiency ( η = 83.41%), indicating the general efficacy of our surface cation-defects engineering strategy, which provided new insights for the design of ceramic components.
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APPLIED PHYSICS A-MATERIALS SCIENCE & PROCESSING
·
2025
·Vol. 131
·期 3
·196
·
IF 2.8
Zhao Xinyue,Zhang Xijun,Gao Chenyu,Wang Peng,He Yan,Chu Dianming,Bai Wenjuan
Carbon nanotubes (CNT) have attracted much attention due to their wide range of applications, but the complex and time-consuming preparation of CNT by the traditional chemical vapor deposition (CVD) method has limited their large-scale production. In this study, we propose an innovative “one-step” CNT growth process to simplify catalyst preparation. The significant advantage of this method is that it reduces the time consumption by 90% compared with other catalyst preparation methods. Aluminum foil was found to outperform other materials as a substrate and catalyst loading significantly affected the morphology and quality of CNT. High-density, low-defect, high-crystallinity, and high-quality CNT could be grown on aluminum foil at the optimal loading (e.g., 0.10 mol/L.) In addition, the growth of CNT is affected synergistically by catalyst-substrate interfacial interactions, temperature, and aqueous solution content. In particular, CNT arrays with a diameter of 20 nm were successfully prepared on an iron catalyst calcined at 660 °C. These findings are important for large-scale, low-cost production of high-quality CNT.
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WATER AIR AND SOIL POLLUTION
·
2025
·Vol. 237
·期 3
·129
·
IF 3
Han Yuliang,Fang Jiahui,Ji Xinyuan,Hu Sheng
To address the problem of excessive Cd (II) content in selenium-rich soil and water in Enshi region, this experiment utilized ion imprinting for selective adsorption of cadmium, which made the separation of selenium and cadmium possible. A novel attapulgite-based Cd(II) ion-imprinted polymer (AF-IIP) was prepared with the surface imprinting technique, using magnetic attapulgite as the matrix and (3-mercaptopropyl) trimethoxysilane (MPS) as the functional monomer. SEM, XRD, VSM, FT-IR and EDS elemental analysis were performed to confirm the structural transformations and compositional changes of the materials during the preparation process. The optimal pH, optimal time, adsorption kinetics and adsorption isotherms during the adsorption of Cd (II) by ion-imprinted polymers were investigated. The experimental results showed that the optimum pH of the adsorption process was 6.0 and the maximum adsorption amount was 49.0 mg·g −1 . In the presence of competitive ions, the adsorption capacity of AF-IIP for Cd (II) is much larger than that of Se ions, indicating that AF-IIP has good selectivity. The adsorption reaction conformed to the Langmuir isotherm model and kinetic data were more suitable to be explained by pseudo-second-order model. Furthermore, XPS analysis confirmed that Cd(II) adsorption primarily occurs via interactions with thiol (-SH) groups. In addition, regeneration tests have shown that AF-IIP was reusable and stable.
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npj Science of Food
·
2026
·
IF 9.7
Li Ya,Wu Hailin,Liang Jianfeng,Pan Yuhua
HS-SPME-GC/MS and UPLC were used to profile volatiles and non-volatiles in four Liupao tea aroma types (betelnut, stale, ginseng, and jujube). Combined flavoromics and molecular docking clarified aroma differentiating metabolites and their sweet taste binding mechanisms. In total 1509 volatiles across 15 classes were detected; terpenes, esters and ketones dominated. 47 key aroma compounds (ROAV≥100) distinguishing the four types were screened via OPLS-DA. Nonvolatile catechin, gallic acid and theobromine also mediated flavor divergence, with Mantel tests revealing synergistic correlations between catechin compounds and key volatiles. The 12 volatile compounds mainly bind to the T1R2 and T1R3 sweet taste receptors by Van der Waals and electrostatic interactions. Terpenoid and amino acid metabolic pathways dominated aroma differentiation. This work identifies type specific key metabolites and their molecular flavor-forming mechanisms to support Liupao tea aroma classification.
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AAPS PHARMSCITECH
·
2021
·Vol. 22
·期 1
·1-12
·
IF 3.246
Yi Shengfu,Zhang Cong,Hu Junjie,Meng Yan,Chen Liang,Yu Huifan,Li Shan,Wang Guihong,Zheng Guohua,Qiu Zhenpeng
Urolithin A (Uro-A), a metabolite of ellagitannins in mammals’ intestinal tract, displays broad biological properties in preclinical models, including anti-oxidant, anti-inflammatory, and anti-tumor effects. However, the clinical application of Uro-A is restricted because of its low aqueous solubility and short elimination half-life. Our purpose was to develop a delivery system to improve the bioavailability and anti-tumor efficacy of Uro-A. To achieve this goal, urolithin A–loaded PEGylated liposomes (Uro-A-PEG-LPs) were prepared for the first time and its physicochemical properties and anti-tumor efficacy in vitro were evaluated. The morphology of Uro-A-PEG-LPs displayed a uniform sphere under transmission electron microscope. The particle size, polydispersity index, zeta potential, and encapsulation efficiency of Uro-A-PEG-LPs were 122.8 ± 7.4 nm, 0.25 ± 0.16, − 25.5 ± 2.3 mV, and 94.6 ± 1.6%, respectively. Moreover, Uro-A-PEG-LPs possessed higher stability and could be stably stored at 4°C for a long time. In vitro release characteristics indicated that Uro-A-PEG-LPs possessed superior sustained release properties. The results of confocal laser scanning microscopy experiment showed that the coumarin 6–loaded PEGylated liposomes (C6-PEG-LPs) have superior cellular uptake than that of conventional liposomes. In addition, in vitro tests demonstrated that Uro-A-PEG-LPs elevated cytotoxicity and pro-apoptotic effect in human hepatoma cells comparing with free Uro-A. Furthermore, the results of pharmacokinetic experiments showed that the t 1/2 , AUC 0-t , and MRT 0-t of Uro-A-PEG-LPs increased to 4.58-fold, 2.33-fold, and 2.43-fold than those of free Uro-A solution, respectively. Collectively, these manifested that PEGylated liposomes might be a potential delivery system for Uro-A to prolonging in vivo circulation time, promoting cellular uptake, and enhancing its anti-tumor efficacy.
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Journal of Translational Medicine
·
2023
·Vol. 21
·期 1
·1-15
·
IF 7.4
Wan Can,Liu Xiao-Qi,Chen Mei,Ma Hui-Han,Wu Guang-Liang,Qiao Li-Jun,Cai Ye-Feng,Zhang Shi-Jie
Background The disruption of blood-brain barrier (BBB), predominantly made up by brain microvascular endothelial cells (BMECs), is one of the characteristics of Alzheimer’s disease (AD). Thus, improving BMEC function may be beneficial for AD treatment. Tanshinone IIA (Tan IIA) has been proved to ameliorate the cognitive dysfunction of AD. Herein, we explored how Tan IIA affected the function of BMECs in AD.Methods Aβ1–42-treated brain-derived endothelium cells.3 (bEnd.3 cells) was employed for in vitro experiments. And we performed molecular docking and qPCR to determine the targeting molecule of Tan IIA on Sirtuins family. The APPswe/PSdE9 (APP/PS1) mice were applied to perform the in vivo experiments. Following the behavioral tests, protein expression was determined through western blot and immunofluorescence. The activities of oxidative stress-related enzymes were analyzed by biochemically kits. Nissl staining and thioflavin T staining were conducted to reflect the neurodegeneration and Aβ deposition respectively.Results Molecular docking and qPCR results showed that Tan IIA mainly acted on Sirtuin1 (SIRT1) in Sirtuins family. The inhibitor of SIRT1 (EX527) was employed to further substantiate that Tan IIA could attenuate SIRT1-mediated endoplasmic reticulum stress (ER stress) in BMECs. Behavioral tests suggested that Tan IIA could improve the cognitive deficits in APP/PS1 mice. Tan IIA administration increased SIRT1 expression and alleviated ER stress in APP/PS1 mice. In addition, LRP1 expression was increased and RAGE expression was decreased after Tan IIA administration in both animals and cells.Conclusion Tan IIA could promote Aβ transportation by alleviating SIRT1-mediated ER stress in BMECs, which ameliorated cognitive deficits in APP/PS1 mice.
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EUROPEAN JOURNAL OF MEDICAL RESEARCH
·
2025
·
IF 3.4
Lv Saihua,Yang Yuxin,Huang Jianfei
Background Angiogenesis is suggested as pathogenesis of inflammatory bowel disease (IBD). However, how angiogenesis mediates pathogenesis of ulcerative colitis (UC), a common form of IBD, remains elusive. Methods Dextran sulfate sodium salt (DSS)-induced UC mouse models were established. Then, these mice were injected with short hairpin RNA against regenerating family member4 (shREG4), followed by evaluation of weight and disease activity index (DAI). Collected colon tissues underwent hematoxylin and eosin staining and immunohistochemistry to detect morphological changes and microvascular density (MVD). Inflammation cytokines, soluble vascular endothelial growth factor receptor1 (sVEGFR1) and VEGF levels were measured using enzyme-linked immunosorbent (ELISA) assay. Matrix metallopeptidase7 (MMP-7) was measured using western blot. REG4 overexpression plasmid-transfected NCM460 cells experienced quantitative real-time reverse transcription polymerase chain reaction and western blot. Human intestinal microvascular endothelial cells (HIMECs) were co-cultured with NCM460 cells that had been transfected with REG overexpression plasmid and treated with MMP-7 mAb and/or sVEGFR1. MMP-7 or sVEGFR1 levels in both culture medium were tested using ELISA. HIMECs angiogenesis was measured by Angiogenesis kit. Results In vivo, DSS-induced mice exhibited lower weight and higher DAI. Collected mice colon tissues showed enhanced inflammation, morphological changes, MVD, and expressions of VEGF, REG4 and MMP-7, which were all reversed after DSS-induced mice injected with shREG4. In vitro, REG4 overexpression-transfected NCM460 cells and its culture medium presented higher MMP-7 level. After co-culture with REG4 overexpression-transfected NCM460 cells, HIMECs culture medium demonstrated lower level of sVEGFR1 and higher angiogenesis, and the latter was reversed by MMP-7 mAb and/or sVEGFR1 treatment in NCM460 cells. Conclusion REG4 induces angiogenesis in UC by upregulating MMP-7 and downregulating VEGFR1.
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CHROMATOGRAPHIA
·
2025
·
IF 1.3
Huang Zhongping,Zou Jianfeng,Zhang Junhao,Wang Haodong,Liu Huijun
In this study, quaternized cationic polyelectrolytes were successfully decorated on polystyrene-divinylbenzene (PS-DVB) microspheres in deep eutectic solvents (DESs). Quaternized cationic polyelectrolytes and hyperbranched layers were prepared using 1,4-butanediol diglycidyl ether (BDDE) and methylamine (MA) as monomers. The effects of different reaction solvents, the amount of agglomerated polyelectrolytes, and the number of hyperbranched layers on the chromatographic performance of the stationary phase were investigated. The surface morphological feature and chemical composition of the PS-DVB microspheres were characterized by SEM, FT-IR, and elemental analysis. The results showed that the optimized anion exchanger had a good chromatographic performance by choosing a mixture of 2.0 mmol/L sodium carbonate and 2.0 mmol/L sodium bicarbonate as the mobile phase at a flow rate of 1.0 mL/min. Seven common anions (fluoride ion, chloride ion, nitrite ion, bromide ion, nitrate ion, phosphate ion, and sulfate ion) were well separated within 25 min. The average numbers of theoretical plates per meter for three batches of columns prepared in DESs increased by 5% ~ 20%, as compared with those of the columns prepared in deionized water. Additionally, the asymmetry factors of detected anions were also improved for the columns prepared in DESs.
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Nature Communications
·
2025
·
IF 15.7
Qiu Liang,Yao Lin,Wang Ping,Jiang Zhiwei,Li Jinglin,Li Ying,Li Yixin,Wu Zhaosong,Nasir Muhammad Salman,Wang Ding,Wang Xinqiang,Huang Zhen,Zhou Baowen
Hydrogen extraction from liquid hydrogen carriers is a promising strategy to address hydrogen storage and transportation challenges for a hydrogen economy. We report a novel heterogeneous catalytic architecture, Ni(Mn)-O-P/GaN nanowires, for efficient, selective, and ultra-stable hydrogen evolution from formic acid (FA). The catalyst achieves a high activity of 29.92 mol H 2 ·g cat −1 ·h −1 with nearly 100% selectivity and a high turnover frequency (TOF) of 31,019.2 h −1 at 150 °C. It exhibits exceptional stability over 4000 hours under fluctuated temperatures (55-75 °C) with a turnover numbers (TONs) of 5,023,060, integrable with low-grade industrial waste heat. In-situ characterizations, isotope experiments, and density functional theory calculations collectively reveal that the synergy between Ni(Mn)-O-P and GaN are favorable for the O-H dissociation of FA with an interesting H-exchange mechanism with H 2 O while inhibiting the undesired FA dehydration and coking formation. An industrial prototype test validates practical on-demand hydrogen production using waste heat.
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