ACS Applied Materials & Interfaces
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2018
·
IF 8.097
Mengmeng Xu,Hui Zhou,Yanan Liu,Jing Sun,Wenjie Xie,Ping Zhao,Jie Liu
Alzheimer’s disease (AD) has become one of the most serious societal problems globally, with no effective treatments. Parenchymal accumulation of amyloid beta (Aβ) plaques and the formation of neurofibrillary tangles are the hallmarks of AD. Their possible interactions and synergistic effects in AD have been gradually elucidated. The failure of many clinical trials suggests that it is difficult to treat AD with a focus on a single target. Instead, multiple targets may be an important direction for AD drug research. In this study, we used protoporphyrin IX (PX)-modified oxidized mesoporous carbon nanospheres (OMCN) (PX@OMCN@PEG(OP)@RVGs) as a novel AD multifunctional nanodrug having multiple targets. The nanodrug efficiently inhibits tau phosphorylation. In addition, the use of PX with focused ultrasound triggered the production of reactive oxygen species that significantly inhibited Aβ aggregation. Both approaches notably increased the cognitive level of APP/PS1 transgenic (Tg) mice and ultimately achieved dual-target inhibition of AD. Furthermore, the safe and effective delivery of PX across the blood–brain barrier (BBB) due to modification of the RVG peptide was demonstrated in vivo and in vitro. The favorable photothermal effect of the nanoparticles improved the BBB permeability of PX@OP@RVGs under near-infrared irradiation. The results demonstrated that the novel PX@OP@RVG multifunctional nanomedicine has a dual-target treatment capability for AD and can traverse the BBB, indicating the potential for the effective treatment of AD.
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高影响因子
INTERNATIONAL JOURNAL OF FOOD SCIENCE AND TECHNOLOGY
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2018
·Vol. 54
·期 2
·480-489
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IF 2.383
Dianyu Yu,Ruchun Zhang,Yuqi Wang,Dezhi Zou,Tingting Li,Honglin Tang,Lianzhou Jiang,Liqi Wang
Summary β-Carotene 15,15′-monooxygenase was isolated and purified from the intestinal mucosa of pigs, and then, it was applied to hydrolyse the pigment in soybean oil, and thus, vitamin A fortified soybean oil was obtained. The pig intestinal mucosal protein solution was purified to a specific activity of 2.487 × 10 −4 U mg −1 , maximum reaction velocity ( V max ) of 8.42 × 10 −9 mol/h and Michaelis constant (K m ) of 2.03 × 10 −5 m . The protein solution had a molecular mass of 156 kDa by gel filtration. The sodium deoxycholate concentrations, optimum pH, Tween 40 amount and enzyme amount for vitamin A production in soybean oil were determined to be 6.0 m m , 8.0, 3.0% (w/v) and 0.2 U/mL enzyme, respectively. Under these conditions, β-carotene 15,15′-monooxygenase produced 14.65 mg/L vitamin A after 20 h, with a conversion yield of β-carotene of 33.29% (w/w). Therefore, the nutrients in soybean oil were improved.
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INTERNATIONAL JOURNAL OF BIOLOGICAL MACROMOLECULES
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2018
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IF 3.909
Lu Zhao,Youzhi Zhou,Song Qin,Panpan Qin,Jianlin Chu,Bingfang He
β-Galactosidases can catalyze the hydrolysis of lactose and the synthesis of galacto-oligosaccharides (GOS) from lactose. The catalytic activity of β-galactosidases is usually inhibited by galactose and glucose, which hampers the complete hydrolysis of lactose in food products. In this report, a β-galactosidase (denoted as BMG) from Bacillus megaterium YZ08 without the inhibition by galactose and glucose is the first to our knowledge reported. Efficient secretory expression of BMG was successfully achieved in Bacillus subtilis WB800. With the increasing of galactose and glucose, the activity of BMG dramatically enhanced. With addition of 0.6 M galactose or 1 M glucose, the initial activity to o NPG hydrolysis was increased by 2.1-fold and 4.3-fold, respectively. Moreover, the synthetic rate of galacto-oligosaccharides was enhanced by 1.1-fold and 1.9-fold with the addition of 0.7 M galactose or glucose, respectively. The GOS (278 g/L) from 600 g/L lactose by BMG were efficiently produced within 12 h, and the substrate lactose was completely used up. These results suggest that BMG shows a potential application in the food industry.
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BIOCHEMICAL ENGINEERING JOURNAL
·
2019
·
IF 3.371
Jianguo Zhang,Hongli Mao,Mengla Li,Erzheng Su
An enzymatic approach to β-cyclodextrin synthesis attracts the attention of food engineers and chemists because of the broad applications of β-cyclodextrin in the food industry and the demand for a green process for β-cyclodextrin production. Achieving a high recovery of cyclodextrin glycosyltransferase through immobilization and a high proportion of β-cyclodextrin in a product mixture of cyclodextrins would improve the purification process of β-cyclodextrin. In this study, a recombinant cyclodextrin glycosyltransferase (CGTase) was immobilized on polydopamine-Fe 3 O 4 (PDA-Fe 3 O 4 ) nanoparticles in the presence of polyethyleneimine (PEI). After optimization of the preparation of the PDA-Fe 3 O 4 nanoparticles and the CGTase immobilization step, 77.9% of the CGTase was recovered. The immobilized CGTase exhibited good thermal and operational stabilities, and gave a ratio of 88.9% β-cyclodextrin when the reaction temperature and kinetic parameters were optimized. Immobilized CGTase was recycled nine times and retained 19.1% β-activity and 0% γ-activity. This study has established a reliable approach to the high recovery of CGTase during efficient β-cyclodextrin production.
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Journal of Nano Research
·
2018
·
IF 0.665
Wen Ting Liang,Dan Li,Xue Wen Ma,Wen Juan Dong,Jing Li,Rui Fang Wu,Chuan Dong,Qing Chen Dong
Surface β-cyclodextrin polymer coated Fe3O4 magnetic nanoparticles (NPs) were prepared by anchoring pyromellitic dianhydride derivate-b-cyclodextrin polymer (b-CD-P) onto the surface of Fe3O4 magnetic NPs, which is generated in situ through a co-precipitation method. The transmission electron microscopy (TEM) result indicates that Fe3O4@β-CD-P NPs have an effective average size of 15 ± 2 nm. The surface coated layer β-CD-P was confirmed by Fourier-Transformed Infrared Spectroscopy, and the amount of which was determined to be 138.2 mg g-1 by thermogravimetric analysis. To explore the potential application of such nanocomposites for organic pollutants adsorption, malachite green (MG), a typical cancer-causing water pollutant was evaluated by spectrophotometric method. It was finally learnt that, the adsorption rate of MG by Fe3O4@β-CD-P NPs follows pseudo-second-order kinetics with adsorption isotherm fitted by the Langmuir isotherm model well. The maximum adsorption capacity was measured to be 88.49 mg g-1 at 25 °C. Additionally, a good recyclability of the Fe3O4@β-CD-P was observed over four usage cycles, with slight decrease of adsorption capability.
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ENVIRONMENTAL ENGINEERING SCIENCE
·
2018
·Vol. 35
·期 4
·279-288
·
IF 1.547
Guanghui Wang,Yao Jiang,Suhang Hu,Yadan Guo
Phytoremediation may be used for remediation of cocontaminated soil with heavy metals and polycyclic aromatic hydrocarbons (PAHs). However, the low bioavailability of heavy metals and PAHs in soils may have adverse effects on the efficiency of phytoremediation; it is necessary to find an effective method for simultaneously improving bioavailability of PAHs and heavy metals in cocontaminated soils. In this study, a novel aspartic acid-β-cyclodextrin (ACD) was synthesized, the structural characteristics of ACD were confirmed by FT-IR spectroscopy, and the complexation of fluorene (Flu) and cadmium (Cd) with ACD was studied. Pot culture experiments were conducted to investigate effects of ACD on phytoremediation of soil cocontaminated with Flu and Cd. Results showed that ACD could enhance uptake of Flu and Cd in alfalfa (Medicago sativa L.), and removal percentages of Flu and Cd in planted soil with ACD increased from 45.78% and 25.62% to 66.96% and 34.88%, respectively. Biological activity of planted soils with ACD was obviously higher than that of planted soils without ACD, which resulted in improvement of phytoremediation efficiency. As a promising remediation strategy, ACD-assisted phytoremediation may be a feasible technology for simultaneous removal of PAHs and heavy metals in cocontaminated soils.
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COLLOIDS AND SURFACES A-PHYSICOCHEMICAL AND ENGINEERING ASPECTS
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2018
·
IF 2.829
Xu Hu,Yangchuan Ke,Yi Zhao,Shichao Lu,Chengcheng Yu,Fangfang Peng
Maleic anhydride modified β-cyclodextrin (MAH-β-CD) was copolymerized with acrylamide (AM) and 2-acrylamido-2-methyl propane sulfonic acid (AMPS) to form a copolymer (denoted as AAMC) through redox free-radical polymerization. The copolymer was characterized by FT-IR, 1 H NMR and thermal gravimetric analysis. The silica nanoparticles suspension was mixed with AAMC solution, and their rheological properties were investigated under different experimental conditions. Results showed that AAMC/silica hybrid exhibited viscosity enhancement and better salt-resistance, temperature-tolerance and shear-resistance than AAMC. The AAMC/silica hybrid showed an impressive thermal stability at T = 85 ℃ after 15 days, and the viscosity reached about 2.5 times that of AAMC at 0.8 wt% nanoparticle loading. Dynamic rheology studies indicated that silica nanoparticles acted as physical crosslinkers among polymer molecules so as to enhance the elasticity of the AAMC/silica hybrid. The superior properties of AAMC/silica hybrid indicated that it has the potential to enhanced oil recovery.
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RSC Advances
·
2015
·Vol. 5
·期 88
·71749-71755
·
IF 3.84
Xueni Li,Longzhen Zheng,Yimin Wang,Nan Zhang,Yan Lou,Ting Xiao,Jiani Liu
This paper reports on an experimental research of a 2-hydroxypropyl-β-cyclodextrin (HP-β-CD) enveloped 10-methylphenothiazine (MPT) catalyst (MPT-HP-β-CD) with high sensitivity and wonderful electrical conductivity for the electrochemical detection of reduced glutathione (GSH) in a neutral environment. We obtained a linear response range from 10−6 mol L−1 to 5.8 × 10−4 mol L−1 for GSH with a low detection limit of 2.87 × 10−7 mol L−1, providing a potentially usefulness in the development of sensors for non-enzyme detection of GSH.
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JOURNAL OF COLLOID AND INTERFACE SCIENCE
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2013
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IF 3.172
Yan Zhao,Bing Yan
GaN-functionalized rare earth (Eu 3+ and Tb 3+ ) organic/inorganic mesoporous nanocomposites have been successfully synthesized (designated as RE(L SBA-15) 3 (L GaN); RE = Eu, Tb; L = TAA Si, BTA Si). The organosilane precursor materials (L SBA-15) are synthesized by co-condensation of tetraethylorthosilicate (TEOS) and the functionalized β-diketones (TAA Si and BTA Si) in the presence of Pluronic P123 surfactant as a template. The modified β-diketones ligands are also used to covalently bond with surface-modified GaN and formed another precursor L GaN. Both of the precursors can coordinate with rare earth ions to synthesize the final mesoporous materials via a sol–gel process. FTIR, TEM, XRD, and nitrogen (N 2 ) adsorption/desorption measurements are employed to characterize the mesostructure of RE(L SBA-15) 3 (L GaN). The luminescence properties and thermogravimetric analysis of all the prepared materials are characterized in detail, and the results reveal that a series of uniformed mesopore structure hybrid materials has been achieved. The mesoporous material Eu(BTA Si SBA-15) 3 (BTA Si GaN) has better luminescence intensity, higher quantum efficiency, and longer lifetime than Eu(TAA Si SBA-15) 3 (TAA Si GaN). While the nanocomposite Tb(TAA Si SBA-15) 3 (TAA Si GaN) revealed the strongest characteristic emission of Tb 3+ ions than Tb(BTA Si SBA-15) 3 (BTA Si GaN), the excellent luminescent properties and thermal stability enable the hybrid mesoporous material to have potential applications in optical field.
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高影响因子
JOURNAL OF MOLECULAR CATALYSIS A-CHEMICAL
·
2015
·
IF 3.615
Junmei Liang,Ranran Ding,Yulong Wu,Yu Chen,Kejing Wu,Yongqiang Meng,Mingde Yang,Yaowu Wang
Hydrotreatment of heteroatomic model compounds in microalgae-based bio-oils into diesel-like hydrocarbons was carried out over carbon nanotubes (CNTs)-supported β-Mo 2 C catalyst with superior activity and selectivity under a mild condition (≤200 °C). The results show that stearic acid and hexadecanamide can be completely converted into n -C 15 –C 18 alkanes over β-Mo 2 C/CNTs catalyst. The β-Mo 2 C/CNTs favors the pathway of hydrogenation–dehydration–hydrogenation to produce n -octadecane with an optimal yield of 91.24% at a lower temperature of 180 °C during hydrotreating process of stearic acid. The recycle tests demonstrate that the β-Mo 2 C/CNTs exhibits excellent stability, and can be reused for seven times consecutively without reduction of catalytic stability. Based on the determined products, a brief reaction pathway is proposed. Therefore, a novel approach to produce diesel-like hydrocarbons via catalytic hydrotreatment of microalgae-based bio-oils over β-Mo 2 C/CNTs is introduced, which provides a basic research as well as technical parameters for its further industrialization.
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Nanoscale
·
2015
·Vol. 7
·期 26
·11393-11400
·
IF 7.394
Lin Gan,Fang-Fang Xu,Xiong-Hui Zeng,Zuo-Sheng Li,Zhi-Yong Mao,Ping Lu,Ying-Chun Zhu,Xue-Jian Liu,Lin-Lin Zhang
The critical doping structures of rare-earth atoms in the promising β-SiAlON phosphors have long been argued owing to the lack of direct evidence. Here, the exact locations and coordination of the Ce rare-earth atoms in the β-SiAlON structure have been examined using an atom-resolved Cs-corrected scanning transmission electron microscope. Three different occupation sites for the Ce atoms have been directly observed: two of them are in the structural channel coordinated with six and nine N(O) atoms, respectively; the other one is the unexpected substitution site for Si(Al). The chemical valences and stabilities of the doping Ce ions at the different occupation sites have been evaluated using density functional calculations. Correlation of the different doping structures with the luminescence properties has been investigated by the aid of cathodoluminescence (CL) microanalysis, which verifies the different contribution of the interstitial trivalent Ce ions to the light emission while no luminescence is observed for the substitutional doping of quadrivalent Ce.
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高影响因子
MICROCHIMICA ACTA
·
2014
·Vol. 181
·期 13
·1573-1580
·
IF 3.719
Wang Meilan,Wu Huan,Chi Yuwu,Chen Guonan
Heating and drying of the mixture of glutathione-etched gold nanoparticles (Au-SG) and β-cyclodextrin (β-CD) results in the formation of β-CD-capped and glutathionate-protected Au 13 nanoclusters (Au 13 (SG) 8 @β-CD). Their particle size, composition, and number of gold atoms and the capping molecules were characterized by scanning electron microscopy, fluorescence, UV–vis absorption, FT-IR spectroscopy and mass spectrometry. The fluorescence of these nanoclusters is specifically enhanced by the addition of Ag(I) ions to the aqueous solution. This effect was exploited to develop a selective and sensitive method for the fluorometric determination of Ag(I) in water in the concentration range between 0.5 nM and 0.1 μM, with a detection limit at 0.3 nM (at a signal-to-noise ratio of 3).
β-CD-capped Au 13 nanoclusters has been synthesized by heating and drying the mixture of glutathione-etched gold nanoparticles and β-CD. A simple, sensitive and selective FL sensing method for Ag + in environmental water has developed using the Au nanoclusters.
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