ENVIRONMENTAL SCIENCE AND POLLUTION RESEARCH
·
2021
·Vol. 29
·期 15
·21656-21669
·
IF 4.223
Han Shuang,Xiao Pengfei,An Lu,Wu Dedong
Tetracycline (TC) is a typical ecotoxic antibiotic, which easily causes bacterial resistance. Therefore, it is necessary to remove TC from the water environment. In recent years, advanced oxidation processes (AOPs) rely on the use of highly reactive oxidizing sulfate radical which is turning into an increasingly popular as a tool of the removal of TC. In this study, cobalt-doped pomelo peel carbon composite (Co-PPCC) was prepared by the impregnation coprecipitation method to activate peroxymonosulfate (PMS) to remove TC. SEM, BET, XRD, FTIR, XPS, TGA, and other analytical techniques indicated that a carbon composite catalyst with excellent performance has been successfully prepared. TC was removed by the synergistic effect of adsorption and catalytic degradation processes. The adsorption capacity was limited (only approximately 20% within 60 min) and tending to saturation, which indicated that the removal of TC in the Co-PPCC/PMS system was mainly due to oxidative degradation. The influence of the Co-PPCC and PMS dosage, initial TC concentration, initial pH values, and coexisting anions on the removal efficiency of TC was investigated. When the Co-PPCC catalyst dosage was 1 g/L, PMS concentration was 2 g/L, and pH value was 11, the removal efficiency of TC with a concentration of 50 mg/L reached 99% within 60 min. Free radical quenching experiment and electron paramagnetic resonance (EPR) analysis indicated that the free radical and non-radical degradation processes exist in the Co-PPCC/PMS/TC system. The main degradation products and the possible transformation pathways of TC were explored by LC-MS. In addition, after four cycles of Co-PPCC tests, the removal efficiency of TC can reach 64%. This study provides a new method to reuse abandoned pomelo peels and synthesize an economical and environmentally friendly catalyst for activating peroxymonosulfate to remove TC antibiotics in water.
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JOURNAL OF SOILS AND SEDIMENTS
·
2022
·Vol. 22
·期 7
·2009-2021
·
IF 3.536
Liu Quanfeng,Yu Jiang,Jiang Yinying,Zhong Chengwei,Ding Senxu,Zhou Peng,Jin Yuanxiao
Purpose Soil organic matter pollution is a serious threat to the human living environment. The purpose of this paper is to investigate the effect of MIL-101(Fe)/g-C 3 N 4 catalyzed peroxynitrite degradation of 2-chlorophenol in soil and to analyze the reaction mechanism and further to explore the optimal parameters of this reaction system with utilizing the mathematical model. Methods MIL-101(Fe)/g-C 3 N 4 was successfully obtained by hydrothermal synthesis method by growing MIL-101(Fe) on the surface of g-C 3 N 4 to catalyze the degradation of 2-CP (2-chlorophenol) in soil by sodium persulfate. The influence factors on the removal rate of 2-CP were analyzed by the response surface methodology (RSM) and combined with theoretical calculations. RSM can be used to model continuous variable surfaces with less data and determine the optimal level range. Results MIL-101(Fe)/g-C 3 N 4 had a good catalytic performance due to its good catalytic properties and the adsorption of g-C 3 N 4 on 2-CP in the system and made the best removal rate of 2-CP increase to 91.2%. The correlation of model fitting results was significant, and the verification of its optimal parameters yielded results with an error of only 4.1%. Conclusion Further studies revealed that SO 4 •− and • OH were the reactive radicals, and SO 4 •− was the dominant, which impelled Fe 2+ and Fe 3+ to interconvert in the complex system. Possible degradation pathways were derived by analyzing the intermediates. Generally, this experiment provides some ideas for future research of MOFs in soil organic pollutant removal systems. Graphical abstract
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APPLIED MICROBIOLOGY AND BIOTECHNOLOGY
·
2021
·Vol. 105
·期 5
·1913-1924
·
IF 4.813
Wei Xinlei,Li Qiangzi,Hu Congcong,You Chun
Abstract D-Mannitol (hereinafter as mannitol) is a six-carbon sugar alcohol with diverse applications in food and pharmaceutical industries. To overcome the drawbacks of the chemical hydrogenation method commonly used for mannitol production at present, there is a need to search for novel prospective mannitol production strategies that are of high yield and low cost. In this study, we present a novel approach for the stoichiometric synthesis of mannitol via an in vitro synthetic enzymatic biosystem using the low-cost starch as substrate. By dividing the overall reaction pathway into three modules which could be executed sequentially in one pot, our design aimed at the stoichiometric conversion of starch-based materials into mannitol in an ATP-independent and cofactor-balanced manner. At optimized conditions, high product yields of around 95-98% were achieved using both 10 g/L and 50 g/L maltodextrin as substrate, indicating the potential of our designed system for industrial applications. This study not only provides a high-efficient strategy for the synthesis of mannitol but also expands the product scope of sugar alcohols by the in vitro synthetic enzymatic biosystems using low-cost starch-based materials as the input.
Key points
• We described a design-build-test-learn pipeline to construct in vitro biosystems.
• The designed system comprised six key enzymes and another three enzymes.
• The system converted maltodextrin stoichiometrically to mannitol in one pot.
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Science China-Chemistry
·
2021
·Vol. 64
·期 4
·616-628
·
IF 9.445
Zhang Lu,Zhang Chi,Zhuang Ze-Nan,Li Chu-Xin,Pan Pei,Zhang Cheng,Zhang Xian-Zheng
Gout, a common type of inflammatory arthritis resulting from chronic elevation of uric acid (UA) level, is usually accompanied with hyperuricemia. The current clinical drugs for the treatment of gout and hyperuricemia are greatly restricted by their limited therapeutic efficacy, off-target toxicity and severe side effects. Here, a neutrophil membrane (NM)-coated and cascade catalytic nanoenzyme was devised with excellent inflammation targeting, inflammatory cytokines neutralizing and UA degrading characteristics for the treatment of gout and hyperuricemia with high efficacy and safety. This nanoenzyme was fabricated by encapsulating uricase (UOx) and catalase (CAT) into zeolitic imidazolate framework-8 (ZIF-8) and further coating it with NM. The NM-coated nanoenzyme inherits the antigenic exterior and cell membrane functions of neutrophils for inflammation targeting and cytokine neutralization. UOx and CAT encapsulated in ZIF-8 could efficiently degrade UA and eliminate hydrogen peroxide (H 2 O 2 ) via a biocatalytic cascade, thus successfully blocking the inflammation amplification during gout development. Both in vitro and in vivo experiments demonstrated the preferable potentials of the nanoenzyme for gout targeting, inflammation elimination and UA degradation, exhibiting great promise for the treatment of gout and hyperuricemia in an effective and safe manner.
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JOURNAL OF NANOBIOTECHNOLOGY
·
2023
·Vol. 21
·期 1
·1-14
·
IF 10.2
Ren Yiming,Chen Yi,Chen Wei,Deng Haotian,Li Peiqi,Liu Yubo,Gao Cangjian,Tian Guangzhao,Ning Chao,Yuan Zhiguo,Sui Xiang,Liu Shuyun,Guo Quanyi
Successful biomaterial implantation requires appropriate immune responses. Macrophages are key mediators involved in this process. Currently, exploitation of the intrinsic properties of biomaterials to modulate macrophages and immune responses is appealing. In this study, we prepared hydrophilic nanofibers with an aligned topography by incorporating polyethylene glycol and polycaprolactone using axial electrospinning. We investigated the effect of the nanofibers on macrophage behavior and the underlying mechanisms. With the increase of hydrophilicity of aligned nanofibers, the inflammatory gene expression of macrophages adhering to them was downregulated, and M2 polarization was induced. We further presented clear evidence that the inflammasome NOD-like receptor thermal protein domain associated protein 3 (NLRP3) was the cellular sensor by which macrophages sense the biomaterials, and it acted as a regulator of the macrophage-mediated response to foreign bodies and implant integration. In vivo, we showed that the fibers shaped the implant-related immune microenvironment and ameliorated peritendinous adhesions. In conclusion, our study demonstrated that hydrophilic aligned nanofibers exhibited better biocompatibility and immunological properties.
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BIOTECHNOLOGY LETTERS
·
2023
·Vol. 45
·期 10
·1327-1337
·
IF 2.7
Yi Jing,Zhang Maifei,Zhu Lin,Xu Changzhi,Li Binglin,Wu Panpan,Wu Hang,Zhang Buchang
Purpose Viruses, such as Ebola virus (EBOV), evolve rapidly and threaten the human health. There is a great demand to exploit efficient gene-editing techniques for the identification of virus to probe virulence mechanism for drug development. Methods Based on lambda Red recombination in Escherichia coli ( E. coli ), counter-selection, and in vitro annealing, a high-efficiency genetic method was utilized here for precisely engineering viruses. EBOV trVLPs assay and dual luciferase reporter assay were used to further test the effect of mutations on virus replication. Results Considering the significance of matrix protein VP24 in EBOV replication, the types of mutations within vp24 , including several single-base substitutions, one double-base substitution, two seamless deletions, and one targeted insertion, were generated on the multi-copy plasmid of E. coli . Further, the length of the homology arms for recombination and in vitro annealing, and the amount of DNA cassettes and linear plasmids were optimized to create a more elaborate and cost-efficient protocol than original approach. The effects of VP24 mutations on the expression of a reporter gene (luciferase) from the EBOV minigenome were determined, and results indicated that mutations of key sites within VP24 have significant impacts on EBOV replication. Conclusion This precise mutagenesis method will facilitate effective and simple editing of viral genes in E. coli .
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MICROCHIMICA ACTA
·
2020
·Vol. 187
·期 6
·1-9
·
IF 6.232
Li Xiaomeng,Kong Weisu,Qin Xia,Qu Fengli,Lu Limin
A facile and sensitive self-powered cathodic photoelectrochemical (PEC) aptasensor is reported for the detection of prostate-specific antigen (PSA) based on CuO-Cu 2 O nanowire array grown on Cu mesh (CuO-Cu 2 O NWA/CM) as electrode. The mixed narrow band gaps of the CuO-Cu 2 O heterostructure ensured its wide absorption band, effective electron/hole separation, and high photocatalytic activity in the visible region. In addition, nanowires directly grown on the substrate provided high specific surface area and exposed abundant active sites, thus guaranteeing its high photocatalytic efficiency. Therefore, the self-powered sensor exhibited favorable analytical performance with fast response, wide linear ranges of 0.01 to 5 ng/mL and 5 to 100 ng/mL, an acceptable detection limit of 3 pg/mL, and reasonable selectivity and stability. The proposed CuO-Cu 2 O NWA/CM can be considered a promising visible light-responsive photoactive material for fabrication of PEC aptasensor with high performance.
a Schematic illustration of construction process of PEC sensing platform based on the CuO-Cu 2 O composite for PSA detection. b Schematic mechanism of the operating PEC system.
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APPLIED BIOCHEMISTRY AND BIOTECHNOLOGY
·
2021
·Vol. 195
·期 4
·2432-2450
·
IF 2.926
Zhang Yueqi,Ding Liping,Yan Zhenzhen,Zhou Dandan,Jiang Junwei,Qiu Jiarong,Xin Zhihong
An alkaline esterase, designated as EstXT1, was identified through functional screening from a metagenomic library. Sequence analysis revealed that EstXT1 belonged to the family VIII carboxylesterases and contained a characteristic conserved S-x-x-K motif and a deduced catalytic triad Ser56-Lys59-Tyr165. EstXT1 exhibited the strongest activity toward methyl ferulate at pH 8.0 and temperature 55°C and retained over 80% of its original activity after incubation in the pH range of 7.0–10.6 buffers. Biochemical characterization of the recombinant enzyme showed that it was activated by Zn 2+ and Co 2+ metal ion, while inhibited by Cu 2+ and CTAB. EstXT1 exhibited significant promiscuous acyltransferase activity preferred to the acylation of benzyl alcohol acceptor using short-chain p NP-esters (C2-C8) as acyl-donors. A structure–function analysis indicated that a WAG motif is essential to acyltransferase activity. This is the first report example that WAG motif plays a pivotal role in acyltransferase activity in family VIII carboxylesterases beside WGG motif. Further experiment indicated that EstXT1 successfully acylated cyanidin-3-O-glucoside in aqueous solution. The results from the current investigation provided new insights for the family VIII carboxylesterase and lay a foundation for the potential applications of EstXT1 in food and biotechnology fields.
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ANALYTICAL AND BIOANALYTICAL CHEMISTRY
·
2018
·Vol. 410
·期 23
·5871-5878
·
IF 3.307
Wang Yu,Cui Min,Jiao Mingxia,Luo Xiliang
Accurate detection of protein biomarkers in complex media remains a challenge due to severe nonspecific adsorption and biofouling, and sensing interfaces that combine the high sensitivity and antifouling ability are highly desirable. Herein, an antifouling sensing interface capable of sensitively assaying immunoglobulin E (IgE) in biological samples was constructed. The sensing interface was fabricated through the self-assembly of a zwitterionic peptide and the IgE aptamer onto a macroporous Au substrate, which was electrochemically fabricated with the aid of multilayer polystyrene nanospheres self-assembled on glassy carbon electrode. Due to the huge surface area arising from porous morphology and high specificity of aptamer, the developed electrochemical biosensor exhibits ultrahigh sensitivity and selectivity towards IgE, with the linear range of 0.1–10 pg mL −1 , and a very low limit of detection down to 42 fg mL −1 . Interestingly, owing to the presence of the zwitterionic peptide, the biosensing interface can satisfyingly reduce the nonspecific adsorption and fouling effect. Consequently, the biosensor was successfully applied to detect IgE in complex biological samples, indicating great promise of this peptide-based sensing interface for antifouling assays.
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MICROCHIMICA ACTA
·
2018
·Vol. 185
·期 3
·1-9
·
IF 5.705
Wang Wei,Jayachandran Silambarasan,Li Mengru,Xu Shenghao,Luo Xiliang
An electrochemical biosensor for the detection of microRNA was prepared via chemical grafting of a Methylene Blue labeled reporter (MB-Rep) duplex onto a nanostructured surface that was obtained by electrodeposition of cobalt oxide and poly(o-phenylenediamine). This is followed by the attachment of hyaluronic acid and gold nanoclusters. In the presence of the target (microRNA), the probe-target duplex and the MB-Rep hairpin are formed. These will displace the labeled reporter from the sensor surface, and this results in a decrease of the amperometric signal for MB at a typical working voltage of −0.28 V (vs. Ag/AgCl). The electrode modified with hyaluronic acid possesses a large electroactive surface area and an excellent antifouling property. This makes it useful for ultrasensitive quantitation of microRNA even in complex biological media. The sensor has a linear response in the 100 f. to 0.1 μM microRNA concentration range, and a 33.3 f. detection limit. It was successfully applied to the determination of microRNA in cancer cells.
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Human Cell
·
2022
·Vol. 35
·期 5
·1577-1590
·
IF 4.374
Hu Yixiang,Zhang Wenyou,Liu Zheng,Xing Qichang,Liu Renzhu,Yan Qingzi,Li Wencan,Liu Xiang
Cervical cancer is one of the most frequent types of cancer in women, which is characterized by high invasion and metastatic tendency in its advanced stage. Emerging evidence indicated that long non-coding RNAs (LncRNAs) are involved in the pathogenesis of cervical cancer. LINC01287 has been reported to play crucial regulatory roles in the pathogenesis and progression of multiple cancers. However, up until now, whether LINC01287 is associated with the initiation and development of cervical cancer remains largely unknown. In the present study, expression levels of LINC01287, miR-513a-5p and stress-associated endoplasmic reticulum protein 1 (SERP1) mRNA were quantified utilizing qRT-PCR. A series of functional experiments including CCK-8 assay, colony formation assay, transwell assay, flow cytometry, and tumor xenograft growth of cervical cancer cells were performed for studying the effects of LINC01287. The luciferase reporter assay, pull-down assay, and western blot were used to confirm the downstream targets of LINC01287 and miR-513a-5p. The results demonstrate that LINC01287 was highly expressed in cervical cancer tissue samples and cell lines. High LINC01287 predicts a poor prognosis for cervical cancer patients. Additional gain- and loss-of-function experiments demonstrated that silencing LINC01287 inhibited cervical cancer cells proliferation, colony formation, migration, apoptosis in vitro and retarded tumor growth and metastasis in vivo. Furthermore, the dual-luciferase reporter gene system and RNA pulldown assay validated that LINC01287 positively regulated SERP1 expressions by sponging miR-513a-5p, and LINC01287 inhibited cervical cancer progression by regulating miR-513a-5p/SERP1 axis. In conclusion, the current study first identified that LINC01287/miR-513a-5p/SERP1 axis played an important role in cervical cancer progression. LINC01287 might be a prognostic biomarker and a target for new therapies in patients with cervical cancer.
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MICROCHIMICA ACTA
·
2017
·Vol. 185
·期 1
·1-9
·
IF 4.58
Zhu Tingfeng,Hu Yongjun,Yang Kang,Dong Ning,Yu Meng,Jiang Ningjing
The authors report on a surface-enhanced Raman scattering (SERS) nanoprobe for the specific detection of Escherichia coli O157:H7 ( E. coli O157:H7). Gold nanoparticles (AuNPs) were coated with a thick silica shell, and the Raman reporter (4,4′-dipyridyl) was embedded between gold nanoparticle and silica shell. This technique prevents any external effects on the AuNPs from the environment and avoids any interaction between the Raman reporter and possible impurities. Compared to the SERS nanoprobe of the conventional AuNP/AgNP type, the present nanoprobe can be applied in complex biochemical circumstances without aggregation because of its excellent stability. The SERS nanoprobeis stable for up to 50 h. The probe was applied to the SERS detection of E. coli O157:H7 by exploiting the Raman band peaking at 1612 cm −1 of 4,4′-dipyridyl (DP). The limit of detection is as low as 10 CFU·mL −1 . The technique was successfully employed to the detection of E. coli O157:H7 in real samples, achieving recoveries between 95.5 and 114.8%.
A novel surface-enhanced Raman scattering (SERS) nanoprobe based on the silica encapsulated gold nanoparticles (SEGNs) combining with the functionalized magnetic nanoparticles (MNPs), detecting E. coli O157:H7 with high sensitivity and specificity.
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