查找使用阿拉丁产品的科研文献

Eupalinolide B alleviates corticosterone-induced PC12 cell injury and improves depression-like behaviors in CUMS rats by regulating the GSK-3β/β-catenin pathway

Tian-tian Wang,Meng-yuan Zhou,Xue-na Gong,Yan Huang,Fei-long Li,Sheng-long Gu,Man-yu Zhang,Ling-ling Li,Ze-shan Xu,Rong Li,Li Cai

Eupalinolide B (EB), a primary bioactive compound isolated from Eupatorium lindleyanum DC ., has exhibited various pharmacological properties, such as antitumor, anti-inflammatory, and notably, neuroprotective effects in neurodegenerative diseases. However, the in-depth studies on the antidepressant potential of EB and its underlying mechanisms are still lacking. Herein, we investigated the therapeutic effects of EB on corticosterone (CORT)-induced neurotoxicity in PC12 cells and its antidepressant-like effects in rats subjected to chronic unpredictable mild stress (CUMS). In particular, we focused on the molecular mechanisms related to modulating the GSK-3β/β-catenin pathway. Our findings revealed that EB promoted cell proliferation while decreasing apoptosis and oxidative stress in CORT-induced PC12 cells. In vivo, EB alleviated the depressive-like behaviors in CUMS rats, as assayed by the sucrose preference test, open field test, and forced swim test. Additionally, EB attenuated the hippocampal pathological damage and increased Ki67- and doublecortin-positive cell numbers in hippocampal dentate gyrus, thus restoring hippocampal neurogenesis in CUMS rats. The binding of EB to GSK-3β was confirmed using molecular docking and cellular thermal shift assays. Overexpression of GSK-3β diminished the therapeutic effects of EB on CORT-induced PC12 cells, further indicating that GSK-3β is the target of EB. Mechanistically, EB hindered GSK-3β activity and thus activated β-catenin signaling in both CORT-induced PC12 cells and CUMS rat hippocampus, as demonstrated by increased p-GSK-3β (Ser9), reduced p-β-catenin, and elevated β-catenin expression. Collectively, this study offers new insights into the antidepressant mechanisms of EB, highlighting its potential as a candidate for depression treatment.

The involvement of lidocaine in amyloid-β1-42-dependent mitochondrial dysfunction and apoptosis in hippocampal neurons via nerve growth factor-protein kinase B pathway

Guo Jianlian,Xu Yong,Liu Jie,Hou Xueqi

This project is conceived to reveal the role of lidocaine in the process of Alzheimer’s disease (AD) and its possible downstream targets. After the employment of AD cell model in mice hippocampal neuronal HT-22 cells in the presence of amyloid-β1-42 (Aβ1-42), Cell Counting Kit-8 method investigated cell viability. Oxidative damage was assayed based on a dichloro-dihydro-fluorescein diacetate fluorescent probe and commercially available kits. The 5,5′,6,6′-tetrachloro-1,1′,3,3′-tetraethylbenzimidazolocarbocyanine iodide fluorescent probe estimated mitochondrial function. Terminal-deoxynucleotidyl transferase mediated nick end labeling, western blotting, and immunofluorescence appraised the apoptotic level. Western blot also ascertained the alternations of nerve growth factors (NGF)-protein kinase B (Akt) pathway-related proteins. Aβ1-42 concentration dependently triggered the viability loss, oxidative damage, and apoptosis in HT-22 cells. Lidocaine promoted the viability and reduced the mitochondrial impairment and mitochondria-dependent apoptosis in Aβ1-42-treated HT-22 cells in a concentration-dependent manner. Besides, lidocaine activated the NGF-Akt pathway and NGF absence blocked NGF-Akt pathway, aggravated mitochondrial dysfunction as well as mitochondria-dependent apoptosis in lidocaine-administrated HT-22 cells in response to Aβ1-42. Altogether, these observations concluded that lidocaine might stimulate NGF-Akt pathway to confer protection against mitochondrial impairment and apoptosis in Aβ1-42-mediated cellular model of AD.

Visible light responsive photocatalytic fuel cell with Ce-UiO-66/g-C3N4/Bi2WO6/Ti photoanode for simultaneous degradation of rhodamine B and electricity generation

Qingmei Qiao,Yunlan Xu,Dengjie Zhong,Xihong Ke,Yuqin Yang,Hanlu Zeng

In order to enhance the carrier separation efficiency and redox ability of photoanode, a dual Z-scheme heterojunction Ce-UiO-66/g-C 3 N 4 /Bi 2 WO 6 /Ti photoanode was prepared and assembled with Cu cathode to form PFC. The characterization results show that Ce-UiO-66/g-C 3 N 4 /Bi 2 WO 6 uniformly distributed on the Ti substrate, with average crystallite size of 9.04 nm and average particle size of 33.05 nm, which provides sufficient reactive active sites for RhB degradation. The RhB degradation rate, P max , J sc , V oc and FF of the PFC were 90.9 % (90 min), 7.53 µW cm -2 , 0.152 mA cm -2 , 0.37 V and 0.134, respectively. The turnover frequency (TOF) for RhB degradation by the PFC reached 0.98 h -1 . Fermi energy level analysis shows that a double-Z heterojunction is formed between Bi 2 WO 6 and g-C 3 N 4 , and between Bi 2 WO 6 and Ce-UiO-66, which not only effectively separates photogenerated e - - h + , but also retains the high redox ability of the composite. Moreover, with the introduction of Ce-UiO-66, the composite photoanode has larger specific surface area and wider visible light absorption range. In addition, because of the built-in electric field, the photogenerated electrons can migrate to the surface of composite particles more easily and react with dissolved oxygen to generate more •O 2 - to participate in the degradation of RhB. This work provides a valuable reference for developing PFC photoanode with high efficiency and visible light response.

CircRNA CTNNB1 (circCTNNB1) ameliorates cerebral ischemia/reperfusion injury by sponging miR-96-5p to up-regulate scavenger receptor class B type 1 (SRB1) expression

Chun Chen,Xiaolong Chang,Shifei Zhang,Qi Zhao,Chunyan Lei

Emerging studies show that circRNA catenin beta 1 (circCTNNB1) plays a critical role in cancer. However, the expression and function of circCTNNB1 in cerebral ischemia/reperfusion injury (IRI) have not been reported. The present study discovered that circCTNNB1 and scavenger receptor class B type 1 (SRB1) expression levels were significantly down-regulated in mouse astrocytes (mAS) treated with oxygen glucose deprivation and reperfusion (OGD/R), and similar results were observed in a mouse middle cerebral artery occlusion model. Overexpression of circCTNNB1 alleviated cell apoptosis, oxidative stress and the inflammatory response induced by OGD/R in vitro. Up-regulation of circCTNNB1 increased SRB1 expression levels to protect mAS cells from OGD/R-induced damage. CircCTNNB1 and SRB1 interacted with miR-96-5p, and the overexpression of miR-96-5p efficiently reversed the function of circCTNNB1 in OGD/R-treated mAS cells. CircCTNNB1 protected against cerebral ischemia-reperfusion injury by up-regulating SRB1 in vivo. In conclusion, our findings suggest that circCTNNB1 acts as a competitive endogenous RNA for miR-96-5p to alleviate cerebral IRI, which provides novel evidence that circCTNNB1 and SRB1 may be biomarkers and therapeutic targets for cerebral IRI.

Effects of hydroxylation at C3′ on the B ring and diglycosylation at C3 on the C ring on flavonols inhibition of α-glucosidase activity

Yajuan Qin,Xiaoai Chen,Fei Xu,Chunhe Gu,Kexue Zhu,Yanjun Zhang,Gang Wu,Ping Wang,Lehe Tan

The structure–activity relationship and inhibitory mechanism of flavonols on α-glucosidase were studied by inhibition kinetics, multispectral study, and molecular docking. The flavonols of rutin, quercetin and kaempferol effectively inhibit the activity of α-glucosidase, among which quercetin and rutin showed the strongest and weakest inhibitory abilities, respectively. The inhibitory ability of flavonols was enhanced by hydroxylation at C3′ of B ring, while it was weakened by diglycosylation at C3 of C ring. Remarkably, the quenching affinity and inhibitory ability of flavonols were inconsistent, which was different from the conclusions reported by some previous studies. This may be ascribed to the hydroxyl groups of C3′ of B ring and C3 of C ring. Furthermore, three flavonols were spontaneously bound to α-glucosidase through hydrophobic interactions and hydrogen bonding, which caused the structure and hydrophobic microenvironment of α-glucosidase to change, resulting in significant inhibition of α-glucosidase by flavonols.

A Bruton tyrosine kinase inhibitor-resistance gene signature predicts prognosis and identifies TRIP13 as a potential therapeutic target in diffuse large B-cell lymphoma

Ding Yangyang,Huang Keke,Sun Cheng,Liu Zelin,Zhu Jinli,Jiao Xunyi,Liao Ya,Feng Xiangjiang,Guo Jingjing,Zhu Chunhua,Zhai Zhimin,Xiong Shudao

Bruton tyrosine kinase inhibitor (BTKi) combined with rituximab-based chemotherapy benefits diffuse large B-cell lymphoma (DLBCL) patients. However, drug resistance is the major cause of relapse and death of DLBCL. In this study, we conducted a comprehensive analysis BTKi-resistance related genes (BRRGs) and established a 10-gene (CARD16, TRIP13, PSRC1, CASP1, PLBD1, CARD6, CAPG, CACNA1A, CDH15, and NDUFA4) signature for early identifying high-risk DLBCL patients. The resistance scores based on the BRRGs signature were associated with prognosis. Furthermore, we developed a nomogram incorporating the BRRGs signature, which demonstrated excellent performance in predicting the prognosis of DLBCL patients. Notably, tumor immune microenvironment, biological pathways, and chemotherapy sensitivity were different between high- and low-resistance score groups. Additionally, we identified TRIP13 as a key gene in our model. TRIP13 was found to be overexpressed in DLBCL and BTKi-resistant DLBCL cell lines, knocking down TRIP13 suppresses cell proliferation, promotes cell apoptosis, and enhances the apoptosis effect of BTKi on DLBCL cells by regulating the Wnt/β-catenin pathway. In conclusion, our study presents a novel BRRGs signature that could serve as a promising prognostic marker in DLBCL, and TRIP13 might be a potential therapeutic target for resistant DLBCL.

Ionic-liquid-based ultrasound-assisted extraction combined with countercurrent chromatography and semipreparative LC for the preparation of monoamine oxidase B inhibitors from Pueraria thomsonii

Sainan Li,Yanjie Li,Ying Wang,Ruizhe Li,Huazhou Niu,Chunming Liu,Yuchi Zhang

A simple and efficient method was developed for the rapid screening and identification of ligands for monoamine oxidase B. A new ionic-liquid-based ultrasound-assisted extraction method for medicinal herbs was also developed and validated. In addition, the hyphenated technique of countercurrent chromatography and semipreparative-LC was developed and applied to the isolation of the chemical constituents for Pueraria thomsonii Benth. Three potent monoamine oxidase B inhibitors, namely, daidzein-4′,7-diglucoside (42.2 mg), puerarin 6′′- O -xyloside (88.3 mg), and 3′-hydroxypuerarin (48.5 mg) with purities of 98.2, 96.3, and 97.1%, respectively, were obtained from 500 g of P. thomsonii raw material using semi-preparative high-performance liquid chromatography, whereas 3′-methoxypuerarin (76.2 mg), daidzein-8-C-apiosyl (1→6) glucoside (84.2 mg), and tectorigenin (75.1 mg) with purities of 98.5, 96.4, and 96.8%, respectively, were obtained from 500 g raw material via countercurrent chromatography using a two-phase solvent system comprising n -hexane–ethyl acetate–methanol–water at a volume ratio of 1.85:1.00:0.86:3.69 (v/v/v/v). Then, the anti-Alzheimer activity of the phytochemicals was assessed using a PC12 cell model. Treatment with tectorigenin, daidzein-4′,7-diglucoside, puerarin 6′′- O -xyloside, 3′-hydroxypuerarin, 3′-methoxypuerarin, and daidzein-8-C-apiosyl (1→6) glucoside (100 μg/mL), resulted in cell viabilities of 69.00, 65.81, 59.69, 57.90, 55.61, and 54.59%, respectively ( p < 0.001). The protocol was proved to be very accurate and efficient.

Chinas Unlimited: Making the Imaginaries of China and Chineseness. By Gregory B. Lee. Honolulu: University of Hawai'i Press, 2003. xi, 121 pp. $45.00 (cloth).

Kathleen Erwin

Triplet–triplet energy transfer from Bi3+ to Sb3+ in zero-dimensional indium hybrids via a B-site co-doping strategy toward white-light emission

Qiqiong Ren,Jian Zhang,Maxim S. Molokeev,Guojun Zhou,Xian-Ming Zhang

Low-dimensional metal halides have emerged as promising platforms for the development of new-generation phosphor-converted light emitting diodes (pc-LEDs), in which zero-dimensional (0D) hybrids with lone-pair ns2 states, in particular, show unprecedented competitiveness owing to their fascinating photoluminescence (PL) properties. Herein, we designed a novel 0D indium hybrid, (C20H20P)2InCl5, and proposed a co-doping strategy to incorporate Bi3+ (6s2) and Sb3+ (5s2) ions into this indium hybrid. Widely tunable emissions from blue to red are achieved, which are assigned to the triplet self-trapped excitons (STEs) (3P1 → 1S0) of Bi3+ (476 nm) and Sb3+ (658 nm), respectively. Importantly, an uncommon triplet–triplet energy transfer from Bi3+ to Sb3+ contributes to tunable dual emissions, and enables a single-phase cool white-light emission under ultraviolet (UV) excitation. Moreover, the energy transfer mechanism is discussed clearly by fluorescence photon dynamic analysis and DFT calculations. This work provides a deeper insight into triplet–triplet energy transfer, as well as presents a new model system for tuning the PL behaviours of ns2 configuration dopants.

Crystal structure, bond characteristics and microwave dielectric properties of Ce2[Zr1-x(Sr1/3B2/3)x]3(MoO4)9 (B = Ta, Sb) solid solutions

Hailong Pan,Shijie Yan,Yiyun Zhang,Jialun Du,Xiaoyu Zhang,Pijun Gong,Haitao Wu,Huanrong Tian,Michael Wübbenhorst

A series of Ce 2 [Zr 1- x (A) x ] 3 (MoO 4 ) 9 (A = Sr 1/3 Ta 2/3 , Sr 1/3 Sb 2/3 ; x  = 0.02, 0.04, 0.06, 0.08 and 0.10) (abbreviated as CZ 1- x T x and CZ 1- x S x ) microwave dielectric ceramics were studied systematically with respect to microstructures, sintering behaviors, crystal structures, microwave dielectric properties and infrared vibrational modes. Density measurements and scanning electron microscopy (SEM) reveal the dense and homogeneous microstructures. X-ray diffraction (XRD) results confirm the formation of solid solutions belonging to the hexagonal system with a R 3 ‾ c $R\bar{3}c$ space group, and the lattice volume increases with raising the doping content. Furthermore, the complex chemical bond theory was used to calculate the intrinsic bond parameters, and the correlations between the microwave dielectric properties and intrinsic bond parameters were established. Finally, far infrared (FIR) spectra reveal that the main polarization contribution stems from the absorptions of the phonon oscillations in these two groups of ceramics. Typically, the optimum microwave dielectric properties are achieved for CZ 0.9 T 0.1 and CZ 0.94 S 0.06 ceramics with ε r  = 10.24, Q  ×  f  = 92,009 GHz (at 9.70 GHz), τ f  = −8.84 ppm °C −1 and ε r  = 10.40, Q  ×  f  = 82,696 GHz (at 9.69 GHz) and τ f  = −8.04 ppm °C −1 , respectively. Interestingly, the CZ 0.9 T 0.1 ceramic exhibits the maximum Q  ×  f value in the ion doped Ce 2 Zr 3 (MoO 4 ) 9 ceramics while retaining a good τ f  = −8.84 ppm °C −1 .

Research on the Synergistic Enhancement of Corrosion Resistance and Wear Resistance of Ni–W–B-Based Coatings by Nano CeO2 and PDA Functionalized VC

Xianmin Gong,Shijun Xu,Yiling He,Kaijun Wei,Quangang Chen,Qing Yuan,Yi He,Yi Fan,Xingtao Cheng

Metal-based composite coatings have been widely studied for their excellent properties. In order to improve the properties of nickel tungsten boron (Ni–W–B) matrix, Ni–W–B/PC coatings were prepared by electrodeposition in the presence of polydopamine functionalized VC (P–VC) and cerium oxide (CeO2) nanoparticles. The effect of cerium oxide content in the nanomaterials on the properties of Ni–W–B coatings was investigated. The results showed that the prepared coatings were dense, uniform, and crack-free, and the best performance was achieved at a cerium oxide content of 2 g/L. The addition of CeO2 nanoparticles can fill the tiny defects in the coating and optimize the coating structure. The addition of these two materials refines the size of the coating grains. In terms of coating performance, the corrosion resistance of the composite coating was enhanced with the addition of CeO2 at a certain P–VC concentration, and the total impedance of the composite coating was 24,030 Ω·cm2, which was 4.8 times higher than that of the Ni–W–B coating and 1.7 times higher than that of the Ni–W–B/P–VC coating. In addition, the hardness of the Ni–W–B/PC-2 composite coating reached 1033.93 HV at a CeO2 content of 2 g/L. The wear resistance of this composite coating was also optimized. Compared with the Ni–W–B/P–VC composite coating, the average coefficient of friction was reduced by 8.8%, and the wear resistance of the composite coating was further improved, which was attributed to the synergistic effect of the two materials. Moreover, the Ni–W–B/PC film has excellent hardness and corrosion resistance, which is promising for the application in harsh environments.

Intracellular Uptake of Curcumin-Loaded Solid Lipid Nanoparticles Exhibit Anti-Inflammatory Activities Superior to Those of Curcumin Through the NF-κB Signaling Pathway

Wang Jiao,Zhu Rongrong,Sun Dongmei,Sun Xiaoyu,Geng Zhengsong,Liu Hui,Wang Shi-Long

Curcumin (Cur) is a naturally derived, novel anti-inflammatory agent, but its poor solubility limits its clinical use. The aim of the present study was to encapsulate Cur into solid lipid nanoparticles (SLNs) to improve its anti-inflammatory activity. The Cur-loaded SLNs (Cur-SLNs) were prepared using emulsification and low-temperature solidification methods. In contrast to free Cur, the particles were well dispersed in aqueous medium, showing a narrow size distribution with a range of 55 ± 1.2 nm, a zeta potential value of –26.2 ± 1.3 mV, and a high drug loading efficiency of 37% ± 2.5%. The sustained release of Cur was observed for up to 6 days. The particles displayed enhanced stability in phosphate-buffered saline by protecting the encapsulated Cur against hydrolysis and biotransformation, as well as increasing biocompatibility. Cur-SLNs were more effective than free Cur at reducing the expression levels of several proinflammatory mediators, including inflammatory cytokines (IL-6, TNF-α, and IL-1β) and nitric oxide (NO), under in vitro conditions. By Western blotting, we found that Cur-SLNs were more active than free Cur in inhibiting the LPS-induced activation of the inflammatory transcription factor NF-κB through the suppression of IκB kinase activation. Compared to free Cur, Cur-SLNs had an increased intracellular uptake over time (observed after 24 h) in RAW264.7 cells. Moreover, the Cur-SLNs (≥20 μM) significantly improved RAW264.7 cell viability by inhibiting apoptosis. Thus, these results demonstrated that SLNs could be used as potential anti-inflammatory drug carriers for the treatment of various chronic diseases.