ACS Sustainable Chemistry & Engineering
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2021
·
IF 8.198
Kun Liu,Shengyue Tang,Wenyan Li,Wei Wen,Shan Ding,Mingxian Liu,Hong Li,Changren Zhou,Binghong Luo
To date, designing a perfect poly(d,l-lactide) (PDLLA) bone repair material still remains a major challenge. Inspired by the superior cell affinity, osteogenic activity, and mechanical properties of polysaccharide chitin whiskers, here, positively charged chitin whiskers (HCHW) and negatively charged maleated chitin whiskers (mCHW) are prepared for strengthening and biofunctionalizing the PDLLA membrane via a layer-by-layer (LBL) electrostatic self-assembly method. At a pH value of 4.3, the process of LBL electrostatic self-assembly between the HCHW and mCHW on the membranes is successfully performed, which can be directly proved by the cross-sectional morphology, light transmittance, surface composition, and topography of the membranes. Especially, polydopamine, as an intermediate layer, can greatly enhance the stability and adhesion of the first chitin whisker layer on the PDLLA substrate. Compared with the PDLLA membrane, the mechanical properties of self-assembly membranes are highly enhanced and controllable by adjusting the number of self-assembly whisker layers. Furthermore, in vitro biological evaluations indicate that the HCHW-assembled surface has a higher cell affinity than that of the mCHW-assembled one, which may be attributed to different protein adsorption. Meanwhile, interesting directional cell migration behavior is observed on the HCHW-assembled surface, which can be explained by the guiding effect of surface ordered topology and collective cell migration. These findings provide a new strategy for designing bioactive PDLLA composites with superior and tunable mechanical properties for bone regeneration materials.
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高影响因子
MOLECULES
·
2021
·Vol. 26
·期 19
·5797
·
IF 4.412
Yanyan Lu,Jianbo Wang,Gang Shen,Jiuling Liu,Hongwei Zhu,Junning Zhao,Shitang He
Introduction: A novel analytical method using fast gas chromatography combined with surface acoustic wave sensor (GC-SAW) was developed for rapid determination of the pharmacological volatiles of turmeric (Curcuma longaL.). Methods: The volatile compounds in 20 turmeric samples, collected from different parts and different origins, were assessed by the fast GC-SAW. In addition, gas chromatography–mass spectrometry (GC-MS) was employed to confirm the chemical composition of the main volatiles. The digital fingerprint of turmeric was established and analysed by principal component analysis and cluster analysis. Results: Curcumene (9.1%), β-sesquiphellandrene (5.1%) and ar-turmerone (69.63%) were confirmed as the main pharmacological volatiles of turmeric. The content of ar-turmerone in lateral rhizome turmeric was significantly higher than that of top rhizome and ungrouped turmeric. The contents of curcumene and β-sesquiphellandrene in top rhizome turmeric were higher than those in lateral and ungrouped turmeric. The 20 turmeric samples were divided into four categories, which reflected the quality characteristics of the turmeric from different parts and origins. Conclusion: The GC-SAW method can rapidly and accurately detect pharmacologically volatiles of turmeric, and it can be used in the quality control of turmeric.Keywords:gas chromatography–surface acoustic wave sensor;gas chromatography-mass spectrometry;turmeric;pharmacological volatiles;determination;quality control
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COLLOIDS AND SURFACES A-PHYSICOCHEMICAL AND ENGINEERING ASPECTS
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2021
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IF 4.539
Jun Wang,Sirong Yu,Xiaoli Yin,Liyuan Wang,Guang Zhu,Kang Wang,Quan Li,Jing Li,Xizhen Yang
Inspired by the “lotus effect’’, more and more attention has been paid to the water repellent and self-cleaning surface. Due to its excellent properties, Mg alloy is considered to be one of the most promising engineering materials so far. However, the humid and corrosive environment makes Mg alloy susceptible to corrosion, which greatly limits its wide application. So it is very necessary to improve the properties of Mg alloy through surface treatment. Because of good chemical stability, thermostability and easy preparation, Zeolitic Imidazolate Frameworks (ZIFs) materials have been applied in the field of superhydrophobicity. In addition, in-situ growth has advantages of simple method, convenient operation and no need for expensive experimental equipment. Therefore, in this work, the superhydrophobic coating with cross-like Zeolitic Imidazolate Framework-L (ZIF-L) structure is fabricated on Mg alloy surface via a simple in-situ growth and low energy modification. The superhydrophobic ZIF-L coating on the Mg alloy substrate has excellent water repellency and self-cleaning property, and the stability test results show that it has good performance in thermostability and durability. Besides, the coating keeps superhydrophobic state after being immersed in an alkaline solution for 112 h, showing excellent resistance to alkaline solution. Therefore, the superhydrophobic coating prepared in this work can expand the application of Mg alloy in different service environments and provide a novel way for Mg alloy applying into more fields.
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International Journal of Nanomedicine
·
2021
·
IF 6.4
Qiangqiang Wang,Xiaoke Sun,Xin Huang,Jianmei Huang,Muhammad Waqqas Hasan,RuoFeng Yan,Lixin Xu,Xiaokai Song,Xiangrui Li
Background Hepatocellular carcinoma-associated antigen 59 (HCA59) from excretory/secretory products of Haemonchus contortus is known to have the ability to modulate the functions of host cells. However, its immunogenicities using different nanoparticles adjuvants remain poorly understood. Purpose The study aimed to select an efficient nanoparticle antigen delivery system, which could enhance the immune responses of Haemonchus contortus HCA59 in mice. Methods Here, the immune responses induced by the recombinant protein of HCA59 (rHCA59) with poly-D,L-lactide-co-glycolide (PLGA) nanoparticles, Chitosan nanoparticles, mixture of PLGA and Chitosan nanoparticles (rHCA59-Chitosan-PLGA), and Freund’s complete adjuvant were observed, respectively, in mice. Cytokine and antibody levels induced by different groups were detected by ELISA assay. The effects of lymphocyte proliferations on different groups were examined using CCK-8 kit. Phenotypes of T cells and dendritic cells were analyzed by flow cytometry. Results On day 14 post vaccination, levels of IgM, IgG1, IgG2a, IFN-γ, IL-4, and IL-17 were significantly increased in the groups immunized with rHCA59 encapsulated with nanoparticles. After mice were vaccinated with rHCA59 loaded with Chitosan/PLGA nanoparticles, lymphocytes proliferated significantly. Additionally, the percentages of CD4 + T cells (CD3 + CD4 + ), CD8 + T cells (CD3 + CD8 + ), and dendritic cells (CD11c + CD83 + , CD11c + CD86 + ) were obviously up-regulated in the mice immunized with nanoparticles, especially in the rHCA59-Chitosan-PLGA antigen delivery system group. Conclusion The findings of this research demonstrated that rHCA59-Chitosan-PLGA antigen delivery system could induce higher immune responses in mice model and indicated that rHCA59 might be a good candidate molecule to develop nanovaccines against Haemonchus contortus in future study.
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高影响因子
POLYMER
·
2021
·
IF 4.43
Qianru Wanyan,Yaxin Qiu,Wenting Zhang,Hai Yu,Defeng Wu
Crosslinking opens more possibilities of biodegradable poly ( L -malic acid) (PLMA) as functional materials , and network structure is key to its mechanical states and final applications. In this work, the diols with various chain lengths, including six types of fatty diols and four types of polyethylene glycols , were used to construct networks in PLMA, aiming at establishing network structure-mechanical state-material function correlations. As the molecular weights of diols increased from 100 to 400, the samples experienced evident transition from high-strength plastic to low-loss rubber, finally to soft elastomer . The strengths and Young's moduli varied from 71 MPa to 0.6 MPa and from 2.3 GPa to 2.7 MPa, respectively, accompanied by a broad variation of glass transition temperature ( T g , 50 o C–7 o C). This brittle-ductile transition was revealed in terms of elastoplastic and dielectric relaxations, then. Using the samples with different network structures as components, the actuators with attractive capability to complete asynchronous actions were fabricated. This work proposes promising applications of cross-linked PLMA as functional or smart materials due to its easily-tuned mechanical states and T g , together with its good antibactierial activity and shape memory effect.
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POLYMER
·
2021
·
IF 4.43
Shan Lu,Jinling Li,Fei Liu,Meiling Chen,Haining Na,Jin Zhu
Modification of cellulose homogeneously by graft copolymerization via grafting-from or grafting-to approaches is an attractive and versatile way to impart specific properties onto this promising renewable material. The grafting efficiency is greatly influenced by the solvent and catalyst used during this process. In this study, cellulose- graft -poly ( l -lactic acid) (C- g -PLLA) was prepared by ring opening polymerization of l -lactide (LA) (grafting-from) and transesterification of PLLA (grafting-to) with cellulose dissolved in CO 2 switchable solvent. The impact of the presence of 1,8-diazabicyclo-[5.4.0]-undec-7-ene (DBU) was then elucidated by comparing the grafting efficiency of the process with or without adding extra amount of this organic superbase . Results showed that without adding extra DBU, the grafting efficiency increased with an increase in concentration of grafting agents (LA or PLLA). However, when extra DBU was added, the grafting efficiency decreased with increased levels of DBU. These results indicate that the DBU protonated by the hydroxyl group from cellulose participated in the dissolution of cellulose after capture of CO 2 in the switchable solvent, and played a role in the graft copolymerization to produce C- g -PLLA. On the other hand, the free DBU that was unprotonated displayed an antagonistic effect towards grafting copolymerization . Detailed graft copolymerization pathways and related modes of action were then considered in attempt to provide new insight into the role of CO 2 switchable solvent for cellulose modification by ROP or transesterification .
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BIOMATERIALS
·
2020
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IF 10.317
Zhijian Yang,Yue Xi,Jun Bai,Zhiwei Jiang,Shuqin Wang,Haolan Zhang,Wei Dai,Chaozhen Chen,Zhongru Gou,Guoli Yang,Changyou Gao
The dual functional implants of antibacteria and osteointegration are highly demanded in orthopedic and dentistry, especially for patients who suffer from diabetes or osteoporosis simultaneously. However, there is lack of the facile and robust method to produce clinically applicable implants with this dual function although coatings possessing single function have been extensively developed. Herein, hyperbranched poly-L-lysine (HBPL) polymers were covalently immobilized onto the alkali-heat treated titanium (Ti) substrates and implants by using 3-glycidyloxypropyltrimethoxysilane (GPTMS) as the coupling agent, which displayed excellent antibacterial activity against S. aureus and E. coli with an efficiency as high as 89.4% and 92.2% in vitro , respectively. The HBPL coating also significantly promoted the adhesion, spreading, proliferation and osteogenic differentiation of MC3T3-E1 cells in vitro . Furthermore, the results of a S. aureus infection rat model in vivo ulteriorly verified that the HBPL-modified screws had good antibacterial and anti-inflammatory abilities at an early stage of implantation and better osteointegration compared with the control Ti screws.
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高影响因子
BIOELECTROCHEMISTRY
·
2020
·
IF 4.722
Yumin He,Li Zhou,Lei Deng,Zemeng Feng,Zhong Cao,Yulong Yin
As a primary building block for protein synthesis, l -arginine ( l -Arg) is also a precursor for the synthesis of important metabolites, and is involved in various physiological and pathophysiological processes. l -Arg is a potential biomarker in clinical diagnosis and nutritional status assessment, making it valuable to quantify and monitor this biomolecule. In this study, peptide aptamers that specifically interact with l -Arg were identified by high-throughput molecular docking, and the binding capacities between the synthesized peptide aptamers and l -Arg were then measured by isothermal titration calorimetry. We hypothesized that the peptide aptamer with the greatest binding capacity could be used as the recognition element in a biosensor. A chemosynthetic peptide aptamer modified with mercaptan and spacer units (thioctic acid-GGGG-FGHIHEGY) was thus used to construct label-free electrochemical impedimetric biosensors for l -Arg based on gold electrodes. The optimum biosensor showed good sensitivity to l -Arg with a linear range of 0.1 pM–0.1 mM, and the calculated limit of detection (three times the signal-to-noise ratio) was 0.01 pM. Interference studies and assays of diluted serum samples were also carried out, and satisfactory results obtained. In conclusion, a potential method of peptide aptamer screening and biosensor fabrication for detecting small biological molecules was demonstrated.
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高影响因子
LWT-FOOD SCIENCE AND TECHNOLOGY
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2021
·
IF 4.952
Chuan-Guo Ma,Man Zhao,Tian-Lei Si,Xiao-Wei Chen
Sea buckthorn ( Hippophaë rhamnoides L.) pulp oil (SPO) is enriched in natural bioactive components with high pharmacological activities. However, the presence of polysaccharides affects oil quality. Herein, the effects of different polysaccharide-adsorption treatments on the bioactive components and the in vitro antioxidant activities of SPO were investigated. The results demonstrated that the microwave-assisted activated clay (MAE-ACL) adsorption was more effective than the ultrasound-assisted activated clay adsorption for removing polysaccharides. Although the bioactive component levels and antioxidant activities of the SPO were reduced during the adsorption, the squalene content showed no significant difference. By performing principal component and correlation analyses that further evaluated the connection between the bioactive components and the free radical scavenging activities of SPO, MAE-ACL was determined to be the more suitable polysaccharide-adsorption treatment.
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Molecular Systems Design & Engineering
·
2021
·Vol. 6
·期 10
·825-831
·
IF 4.935
Shaoqing Li,Wei Huang,Wei Tan,Lizhu Zhang,Yanli Zhang,Hongyu Shi,Motilal Mathesh,Colin J. Barrow,Wenrong Yang,Hongbin Wang
Functionalized gold nanoparticles (GNPs) have been extensively studied for cargo delivery and imaging towards biomedical applications. However, there are limited studies showcasing the above two in sync using GNPs. In this work, we fabricated a nanosystem for small molecule delivery and bioimaging based on L-cysteine (L-Cys)-functionalized GNPs (GNP–L-Cys) coupled with a rhodamine 6G derivative (Rh6G2). GNPs conjugated with Rh6G2 (GNP–L-Cys–Rh6G2), as a model system, showcased enhanced fluorescence emission due to “on-demand” release of the rhodamine dye (RGCOOH) from the surface of the GNPs through spiro ring-opening followed by an ion-catalyzed hydrolysis mechanism in the presence of Cu2+. Moreover, in vitro studies showed sustained release of RGCOOH into cells; thus, GNP–L-Cys–Rh6G2 acted as a delivery vehicle without causing significant cell toxicity and was further used for fluorescence imaging. We believe such nanoscale systems with molecular scale engineering open up new possibilities for biomedical applications.
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INTERNATIONAL JOURNAL OF BIOLOGICAL MACROMOLECULES
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2020
·
IF 5.162
Ying Yu,Rongqi Cui,Xin Wang,Hao Yang,Hui Li
Poly( l -lactic acid) (PLLA) has been the most commonly used polymer for making bioresorbable vascular scaffolds (BVS). Despite owning remarkable properties, BVS made from PLLA are facing higher rates of early thrombosis compared with permanent metallic scaffolds. To solve this issue, we modified the PLLA film surface with heparin-mimetic polysaccharide multilayers consisting of sulfated Chinese yam polysaccharide (SCYP) and chitosan (CS) through layer-by-layer (LBL) assembly. The surface chemical compositions, morphologies and growth manner of SCYP/CS multilayers were investigated using X-ray photoelectron spectroscopy, scanning electron microscopy, atomic force microscopy and UV–vis spectroscopy. The relevant hemocompatibility results showed that multilayer-modified PLLA could effectively resist protein adsorption, suppress the platelet adhesion, prolong clotting time, prevent contact and complement activation as well as reduce hemolysis rate. Moreover, the multilayer-modified PLLA exhibited non-cytotoxicity, good antibacterial ability against E. coli and S. aureus , and drug loading/sustained releasing behavior. Overall, the multifunctional PLLA film with integrated properties of hemocompatibility, non-cytotoxicity, antibacterial and drug loading/releasing behavior could be successfully achieved by deposition of SCYP/CS multilayers, which will have potential application in blood-contacting biomedical materials.
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高影响因子
FISH PHYSIOLOGY AND BIOCHEMISTRY
·
2020
·Vol. 46
·期 4
·1207-1218
·
IF 2.249
Deng Dapeng,Yan Xiao,Zhao Wenli,Qin Chaobin,Yang Guokun,Nie Guoxing
Glucose transporter 2 ( glut2 ) has been studied in mammals, aves, and several fish, while the comparative studies of glut2 in common carp are still lacking. In this study, glut2 was firstly isolated and characterized from the liver of common carp. The full-length cDNA of glut2 was 2351 bp with an open reading frame (ORF) of 1512 bp, encoding 503 amino acids. Alignment of glut2 amino acid sequences from different species revealed that common carp glut2 showed higher sequence identity with teleosts, and lower homology with mammals and amphibians. Tissue distribution demonstrated that glut2 mRNA level was mainly expressed in liver, foregut, and midgut. To investigate the actions of glut2 on glucose metabolism, the level of glut2 mRNA was detected after intraperitoneal injection of glucose, human insulin and glucagon (100 ng/g), respectively. Following glucose administration, glut2 gene expression was significantly upregulated at 3 h in the foregut. However, no change was found in hepatic glut2 mRNA level, indicating that glut2 may have a role in intestinal glucose uptake rather than in the liver. Following insulin treatment, the expression of glut2 was markedly downregulated at 3 h and 6 h in the liver, and at 3 h in the foregut, respectively. Furthermore, glut2 mRNA expression was unaffected by glucagon injection in the liver and foregut. These results suggested that the expression of glut2 regulated by pancreatic hormones was different. Taken together, our studies firstly revealed the structure of the glut2 gene and its potential functions in glucose metabolism of common carp.
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高影响因子
危险品化学品经营许可证（带存储）