MICROCHEMICAL JOURNAL
·
2026
·
IF 5.2
Kai Hu,Wuliji Hasi,Guoqiang Fang,Siqingaowa Han
Cinnabar, with low water solubility and poor oral absorption, poses challenges in toxicity assessment. Relying solely on total mercury leads to overestimation, making detection of soluble Hg 2+ essential. This study presents a surface-enhanced Raman scattering (SERS) strategy using Au concave nanocubes (Au CNC) modified with 3-mercaptopropionic acid (3-MPA) and 4-mercaptopyridine (4-MPY), combined with a data-augmented convolutional neural network-partial least squares regression (CNN-PLS) model, for quantifying soluble Hg 2+ in cinnabar and its preparations. 3-MPA binds Hg 2+ , inducing Au CNC aggregation and enhances the SERS signal, while 4-MPY improves sensitivity. To overcome insufficient data in practical, data augmentation was used to expand the SERS dataset, and the performance of CNN, PLS, and CNN-PLS models was compared before and after augmentation. The results indicate that the data-augmented CNN-PLS model performed best, with a coefficient of determination (R 2 ) of 0.999 in the range of 0.001–1 μg/mL and a Mean Squared Error of Prediction (MSEP) of 0.0001. This method was applied to quantify Hg 2+ leaching from cinnabar and four cinnabar-containing preparations after 12 h of immersion in artificial gastric juice. The mean Hg 2+ concentrations from three cinnabar batches were 0.691, 0.557, and 0.546 μg/mL, while for the preparations, the concentrations were 0.085, 0.047, 0.038, and 0.023 μg/mL. These results showed excellent agreement with atomic fluorescence spectrometry (AFS), confirming the method's reliability and accuracy. This simple, accurate, and safe approach offers a novel solution for mercury safety assessment in cinnabar and its preparations, with significant potential for traditional Chinese medicine safety evaluation and environmental monitoring.
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SENSORS AND ACTUATORS B-CHEMICAL
·
2025
·
IF 7.7
Jian Chen,Jiaying Yang,Yanbin Lu,Zhiyuan Dai,Huan Li
Okadaic acid (OA), a diarrhetic shellfish toxin, poses severe health risks to humans and causes substantial economic losses in aquaculture, creating an urgent need for rapid, sensitive, and field-deployable detection technologies for tracing its contamination in shellfish. Herein, we proposed a surface-enhanced Raman scattering (SERS) sensing method for OA in shellfish. The system was constructed by integrating Raman-active aptamer-modified gold/magnetic nanohybrids (Apt-Au/Fe 3 O 4 NHs) with a complementary sequence-modified silver nanoparticle-functionalized bacterial cellulose film (CS-Ag NPs@BC-film). Upon the introduction of OA, it competitively bound with the Apt, leaving the CS-Ag NPs@BC-film after magnetic separation, thereby inducing a reduction in the Raman signal. Sensitive quantification of OA was achieved by measuring the difference in the Raman signal, achieving a detection limit as low as 2.82 × 10 −2 nM (S/N = 3). Analysis of the spiked real samples demonstrated good accuracy and precision of the method. Compared with commercial ELISA kits, the method presented with comparable detection results while offering simplified operational workflows. These findings highlight its potential as a practical tool for monitoring hazardous contaminants in aquatic environments and ensuring seafood safety.
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FOOD CHEMISTRY
·
2025
·
IF 9.8
Guotong Chen,Fang Mi,Shan Zhang,Xuehui Rao,Jian Sun,Zhong Yang,Ming Guan
This study reports the design and fabrication of a magnetic nanoporous carbon-based recyclable surface-enhanced Raman scattering (SERS) substrate Co/C@Ag/g-C 3 N 4 , which can simultaneously achieve SERS detection and visible-light photocatalytic degradation of malachite green (MG). Co/C and g-C 3 N 4 can spontaneously adsorb and concentrate MG near plasmonic nanoparticles, thereby enhancing the SERS response. The substrate exhibited a SERS enhancement factor of approximately 1.3 × 10 6 . The limit of detection (LOD) for MG in aquaculture water, prawn, and tilapia was 8.2 × 10 −11 M, 0.08 μg/kg, and 0.06 μg/kg, respectively. The substrate can be magnetically collected from the complex matrix and regenerated through photocatalytic degradation. Eliminates the co-centrifugation of the SERS substrate with the complex matrix. Even after 4 regeneration cycles, the substrate retained nearly 80 % of its original SERS intensity. This study presents a novel strategy for constructing self-enriched, self-cleaning, and recyclable substrates with promising applications in food safety and environmental protection.
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高影响因子
MICROCHEMICAL JOURNAL
·
2022
·
IF 5.304
Mingchun Lv,Da-Wen Sun,Hongbin Pu,Haofan Zhu
Recyclable surface-enhanced Raman scattering (SERS) substrates are beneficial to cost reduction and environmental protection and have always been a research topic with great interest. Herein, a core–shell-satellite structured SERS substrate Fe 3 O 4 @MIL-100(Fe)@Ag nanoparticles (FMAs) was developed, possessing versatile functionalities of adsorption, detection, degradation and recovery. Results showed that the MIL-100(Fe) with peroxidase-like activity as shell catalyzed H 2 O 2 to generate ·OH degraded pollutants within 5 min to achieve substrate cleaning, making FMAs recyclable for at least 5 times to sequentially detect different cationic dyes with high sensitivity, having low detection limits as low as 15.24 ng/L, 387.87 ng/L and 0.71 ng/g for crystal violet in deionized water, aquaculture water and tilapia, respectively. FMAs also showed good uniformity, reproducibility and stability, with a relative standard deviation value of 5.58 % between substrates and about 78.60 % SERS activity remained after 20 d storage at 4 °C. This FMAs substrate thus has great potential for selective detection of trace cationic dyes in food or other fields.
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SENSORS AND ACTUATORS B-CHEMICAL
·
2022
·
IF 9.221
Xia Zhou,Xiaoling Lin,Haiqiu Huang,Ping Li,Jiamin Wu,Xueqin Huang,Huaihong Cai,Hai Han,Junxia Zheng,Haibo Zhou
Visual, highly selective, and sensitive detection of sulfur dioxide (SO 2 ) and its derivatives (SO 3 2- ) in vitro/in vivo is of great significance in maintaining cell homeostasis. A new Au@Ag@ZIF-8 optical sensor with o-phenylenediamine (OPD) internal coding surface-enhanced Raman scattering (SERS) tags based on the free radical reaction was synthesized for multi-mode detection of sulfite . First, Co 2+ is oxidized to Co 3+ . Second, the oxidoreduction between SO 3 2- and Co 3+ produces SO 4 -• and Co 2+ to complete the cycle. The resulting SO 4 -• is further reacted with the chromogenic substrate OPD to form the polymer. Due to the UV–vis absorbance of OPD polymer being stronger than that of OPD, but the fluorescence intensity is lower, and their Raman peaks are obviously different, this sensor can be used for the detection of SO 3 2- through colorimetric, fluorescence, and Raman multi-mode. The main advantages are as follows: (1) high selectivity; (2) achieve fast, visual, and highly sensitive detection of SO 3 2- , and the low limit of detection (LOD) is 1 nM; (3) improve the reliability of detection and expands the detection range (1 nM-800 μM); (4) demonstrate the applicability in recognizing SO 3 2- in HeLa cells to provide accurate information on the dynamic distribution of sulfur in cells.
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BIOSENSORS & BIOELECTRONICS
·
2022
·
IF 12.545
Xueqin Huang,Zhubao Zhang,Lingzhi Chen,Yongjian Lin,Runmin Zeng,Jun Xu,Shanze Chen,Jianglin Zhang,Huaihong Cai,Haibo Zhou,Pinghua Sun
Ultra-sensitive detection of pathogenic bacteria is of great significance in the early stage of bacterial infections and treatment. In this work, we report a novel strategy using multifunctional Au nano-bridged nanogap nanoparticles (Au NNPs)-based sandwich nanocomposites , that made of Concanavalin A-conjugated Fe 3 O 4 @SiO 2 NPs (ConA-Fe 3 O 4 @SiO 2 NPs)/bacteria/aptamer-modified Au NNPs (apt-Au NNPs), for bacteria discrimination and quantitative detection by surface-enhanced Raman scattering (SERS) and inductively coupled plasma mass spectrometry (ICP-MS), and subsequently photothermal antibacterial assay. The sandwich nanocomposite consists of ConA-Fe 3 O 4 @SiO 2 NPs to magnetically enrich and photothermal killing bacteria , and dual-signal tags of apt-Au NNPs for both SERS sensing and ICP-MS quantification. This strategy can specifically distinguish different kinds of pathogenic bacteria, and provided a good linear relationship of Staphylococcus aureus ( S. aureus ) in the range from 50 to 10 4 CFU/mL with a detection limit of 11 CFU/mL, as well as realized ultralow amounts of bacterial detection in serum sample with high accuracy. Based on the quantitative detection, high antibacterial efficiency was monitored by ICP-MS. Overall, the established method combines bacteria discrimination, quantitative detection, and photothermal elimination with a simple and rapid process, which provides a novel way for the early diagnosis and treatment of bacterial infection.
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MICROCHEMICAL JOURNAL
·
2023
·
IF 4.8
Huan Cheng,Xiaojun Wen,Kuang Luo,Lijun You,Jumei Li
In order to achieve widespread application of surface-enhanced Raman spectroscopy (SERS) technique for practical sensing illegal additives in food, it is crucial to construct a reliable SERS substrate that offers both high sensitivity and reusability. This work utilized a simple and rapid in-situ deposition method to deposit Ag nanoparticles onto hollow Cu 2 O nanospheres, resulting in the formation of Cu 2 O/Ag nanocomposites with photocatalytic and SERS properties. These nanocomposites were employed as a hopeful SERS analysis for effective sensing of colorant additives in fruit juice, including methylene blue (MB) and rhodamine 6G (R6G). Impressively, the nanocomposites exhibited an exceptional detection limit of 10 −9 M for the color agents and demonstrated a strong linear relationship between SERS intensities and the logarithmic concentration (R 2 ≥ 0.95). The hollow structure of Cu 2 O provided low density and long-term dispersion stability in solution, which are beneficial for real-time monitoring the additives in liquid food. The excellent SERS performance can be attributed to the electromagnetic effect of Ag metals and the chemical enhancement of Cu 2 O semiconductors, as confirmed by the N 1 s XPS spectra from MB. Furthermore, the Cu 2 O/Ag nanocomposites efficiently degraded dye molecules under UV light irradiation within a short period of 45 min. So, the nanocomposites exhibited practical recyclability, as demonstrated by their consistent performance over five cycles of SERS sensing. These findings highlight their significant potent for SERS sensing trace pollutants and ecological restoration by photocatalytic reaction in the future.
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APL Bioengineering
·
2023
·Vol. 7
·期 2
·
IF 6
Pan Ting,Zhang Dinghu,Wu Xiaoxia,Li Zihou,Zeng Hui,Xu Xiawei,Zhang Chenguang,He Yiwei,Gong Yuanchuan,Wang Pin,Mao Quanliang,Yao Junlie,Lin Jie,Wu Aiguo,Shao Guoliang
The efficiency of immunotherapy for triple-negative breast cancer (TNBC) is relatively low due to the difficulty in accurately detecting immune checkpoints. The detection of TNBC-related programmed cell death ligand-1 (PD-L1) expression is important to guide immunotherapy and improve treatment efficiency. Surface-enhanced Raman spectroscopy (SERS) and magnetic resonance (MR) imaging exhibit great potential for early TNBC diagnosis. SERS, an optical imaging mode, has the advantages of high detection sensitivity, good spatial resolution, and “fingerprint” spectral characteristics; however, the shallow detection penetration of SERS bioprobes limits its application in vivo . MR has the advantages of allowing deep penetration with no radiation; however, its spatial resolution needs to be improved. SERS and MR have complementary imaging features for tumor marker detection. In this study, gold nanorod and ultrasmall iron oxide nanoparticle composites were developed as dual-modal bioprobes for SERS-MRI to detect PD-L1 expression. Anti-PD-L1 (aPD-L1) was utilized to improve the targeting ability and specificity of PD-L1 expression detection. TNBC cells expressing PD-L1 were accurately detected via the SERS imaging mode in vitro , which can image at the single-cell level. In addition, bioprobe accumulation in PD-L1 expression-related tumor-bearing mice was simply and dynamically monitored and analyzed in vivo using MR and SERS. To the best of our knowledge, this is the first time a SERS-MRI dual-modal bioprobe combined with a PD-L1 antibody has been successfully used to detect PD-L1 expression in TNBC. This work paves the way for the design of high-performance bioprobe-based contrast agents for the clinical immunotherapy of TNBC.
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BIOSENSORS & BIOELECTRONICS
·
2018
·
IF 8.173
Shengping Wen,Yu Su,Rong Wu,Shiwei Zhou,Qianhao Min,Gao-Chao Fan,Li-Ping Jiang,Rong-Bin Song,Jun-Jie Zhu
The accurate therapeutic evaluation for chronic myeloid leukemia (CML) drug is of great importance to minimize side effects and enhance efficacy. Herein, a facile and precise surface-enhanced scattering (SERS) approach based on coupled plasmonic field has been introduced to evaluate the therapeutic outcomes of antileukemia drug through ultrasensitive assay of caspase-3 activity in apoptotic cells. Caspase-3 as an apoptosis indicator could specifically cleave the N-terminus of biotinylated DEVD-peptide (biotin-Gly-Asp-Gly-Asp-Glu-Val-Asp-Gly-Cys) immobilized on the Au nanoparticle-decorated TiO 2 nanotube arrays (TiO 2 /Au NTAs) substrate. After the enzyme cleavage with caspase-3, Raman-labelled Au nanostar (AuNS) probes captured the residual DEVD-peptides via the recognition between streptavidin and biotin , thus resulting in an enhanced Raman response on the SERS platform. The variation of Raman intensity revealed caspase-3 activity that reflected the chemotherapeutic effect. On this platform, AuNS nanoprobes offered a large number of binding sites and intrinsic “hot spots” for Raman reporters, while TiO 2 /Au NTAs rendered a homogenously coupled electromagnetic field between the adjacent repeated units over the large area. In particular, a spatially expanding plasmonic field formed by coupling AuNSs with TiO 2 /Au NTAs would further heighten Raman enhancement. Taking these advantages, the strong and uniform Raman signals were achieved. Furthermore, the practicability investigation witnessed that the proposed SERS strategy was available to evaluate the therapeutic effect of dasatinib on CML K562 cells . The developed method possesses fascinating advantages of cost-effectiveness, excellent reproducibility and high sensitivity, which endows it with promising potential in apoptosis monitoring and anticancer drug development.
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ACS Omega
·
2018
·
IF 0
Kaisong Yuan,Junxia Zheng,Danting Yang,Beatriz Jurado Sánchez,Xiangjiang Liu,Xinjie Guo,Chusheng Liu,Nicoleta Elena Dina,Jingyi Jian,Zhijun Bao,Ziwei Hu,Zhihong Liang,Haibo Zhou,Zhengjin Jiang
Herein, we developed a natural surface-enhanced Raman scattering (SERS) substrate based on size-tunable Au@Ag nanoparticle-coated mussel shell to form large-scale three-dimensional (3D) supercrystals (up to 10 cm2) that exhibit surface-laminated structures and crossed nanoplates and nanochannels. The high content of CaCO3 in the mussel shell results in superior hydrophobicity for analyte enrichment, and the crossed nanoplates and nanochannels provided rich SERS hot spots, which together lead to high sensitivity. Finite-difference time-domain simulations showed that nanoparticles in the channels exhibit apparently a higher electromagnetic field enhancement than nanoparticles on the platelets. Thus, under optimized conditions (using Au@AgNPs with 5 nm shell thickness), highly sensitive SERS detection with a detection limit as low as 10–9 M for rhodamine 6G was obtained. Moreover, the maximum electromagnetic field enhancement of different types of 3D supercrystals shows no apparent difference, and Au@AgNPs were uniformly distributed such that reproducible SERS measurements with a 6.5% variation (613 cm–1 peak) over 20 spectra were achieved. More importantly, the as-prepared SERS substrates can be utilized for the fast discrimination of Escherichia coli, Staphylococcus aureus, and Pseudomonas aeruginosa by discriminant analysis. This novel Au@Ag self-assembled mussel shell template holds considerable promise as low-cost, durable, sensitive, and reproducible substrates for future SERS-based biosensors.
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CHEMICAL ENGINEERING JOURNAL
·
2018
·
IF 6.735
Yue Guo,Yanchun Tao,Xiaowei Ma,Jing Jin,Sisi Wen,Wei Ji,Wei Song,Bing Zhao,Yukihiro Ozaki
Mercuric ion (Hg 2+ ) is a toxic metal ion in the environment, which will seriously damage the people's health. Therefore, the simple sensitive detection of Hg 2+ is of great significance. In this work, Ag-CoFe 2 O 4 /reduced graphene oxide (rGO) nanocomposites were synthesized via a one-pot microwave-assisted reaction, which can directly oxidize 3, 3′, 5, 5′-tetramethylbenzidine (TMB) to produce a light blue. Then we have developed a dual colorimetric and SERS detection of Hg 2+ based on the stimulus of intrinsic oxidase-like catalytic activity of Ag-CoFe 2 O 4 /rGO nanocomposites. It is demonstrated that the interaction between Hg 2+ and Ag nanoparticles can occur in a short time, which includes the formation of Ag-Hg alloy due to the reduction of Hg 2+ . In addition, the formation of such alloy can enhance the oxide-like activity, which makes the detection of Hg 2+ more sensitive. By using the SERS detection approach, the assay can detect Hg 2+ as low as 0.67 nM. This detection ability is much better than previous reports based on the enzyme-like catalytic reaction, which is also lower than the maximum value of Hg 2+ permitted in drinking water by the World Health Organization (WHO) (30 nM) and United States Environmental Protection Agency (EPA) (10 nM). In addition, this detection system also shows an excellent selectivity toward Hg 2+ due to the affinity of Hg to Ag-CoFe 2 O 4 /rGO nanocomposites. Therefore, this approach is potentially applicable for the sensitive determination of Hg 2+ in real environmental conditions.
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TALANTA
·
2024
·
IF 5.6
Yutong Wang,Qianchun Zhang,Mengping Huang,Ganggang Ai,Xiaofeng Liu,Yuqi Zhang,Ran Li,Jie Wu
Stroke ranks as the second leading cause of disability and mortality globally. Biomarker detection represents a promising avenue for predicting disease severity and prognosis. The expression levels of metalloproteinase-9 (MMP-9), neuron-specific enolase (NSE), and N-terminal pro-brain natriuretic peptide (NT-pro BNP) in blood correlate with stroke severity. Hence, monitoring these biomarkers is crucial for stroke diagnosis and management. Point-of-care testing (POCT) offers on-site diagnostic capabilities, with lateral flow immunoassay (LFIA) being the most widely used method currently. However, traditional LFIA sensitivity requires enhancement. This study introduces an ultra-sensitive surface-enhanced Raman scattering-based lateral flow immunoassay (SERS-based LFIA) strip for simultaneous detection of the three stroke biomarkers using SERS immune tags. Bimetallic core-shell structured SERS immune tags leverage the advantages of two metals, ensuring stability and enhancing Raman signals through plasmon resonance. This development of a POCT based on SERS-based LFIA strips offers rapid, sensitive, and multiplex detection of stroke biomarkers. The limits of detection (LODs) for MMP-9, NSE, and NT-pro BNP were 0.00020 ng mL −1 , 0.00016 ng mL −1 , and 0.00012 ng mL −1 , respectively. Furthermore, enzyme-linked immunosorbent assay (ELISA) validated the accuracy of SERS-based LFIA. Clinical sample analysis demonstrated consistency between outcomes obtained by SERS-based LFIA and ELISA. Thus, SERS-based LFIA presents a novel POCT approach for stroke diagnosis.
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