FOOD CHEMISTRY
·
2026
·
IF 10.4
Tong Sun,Wenyue Sun,Shutong Lin,Yan Liu,Lili Lu
α-Galactosidases with high activity and stability are crucial for industrial applications, such as the hydrolysis of raffinose family oligosaccharides (RFOs) in soymilk. In this study, four novel cross-linkers, including dimethyl pimelimidate (DMP), dimethyl suberimidate (DMS), bis(sulfosuccinimidyl)suberate (BS 3 ), and bis- N -succinimidyl-(pentaethylene glycol) ester [BS(PEG) 5 ], were first applied to directly cross-link active α-galactosidase inclusion bodies. This simple approach generated stable and recyclable cross-linked enzyme aggregates (CLEAs) without precipitation or the need for carrier matrices. The resulting CLEAs showed 95–100% activity recovery, far exceeding that of glutaraldehyde (45%). All CLEAs exhibited broad pH and thermal stability, holding over 94% of their initial activity after 10 batches for RFOs hydrolysis in soymilk. Specifically, DMS-CLEAs maintained nearly 100% hydrolysis efficiency throughout 10 cycles. CLEAs prepared with the short-arm cross-linkers demonstrated superior enzyme activity recovery compared to those formed with the long-arm ones. These findings highlight the potential of α-galactosidase CLEAs for legume-based food processing.
✓ 已验证
DOI
PubMed
高影响因子
BIOORGANIC CHEMISTRY
·
2026
·
IF 5.1
Qian Zhang,Wenyu Wang,Chenhan Wu,Qingting Du,Weili Yin,Jiaheng Chen,Jian Ma,Chong Li,Shaojie Zhang
Ulcerative colitis (UC) is characterized by recurrent inflammation of the gastrointestinal tract, and currently available treatment drugs are limited. Plant-derived polysaccharides hold promise for UC prevention. A water-soluble α -D-glucan (CLP80–1) with a molecular weight of 8.17 × 10 5 Da was isolated and characterized from the roots of traditional Chinese medicinal plant Crepis lignea . Structural analysis revealed that its repeating units comprise →6)- α -D-Glc p -(1 → and →3,6)- α -D-Glc p- (1 → residues as the backbone, with α -D-Glc p -(1 → as side chains substituted at C-3. The protective effects of CLP80–1 on dextran sulfate sodium (DSS)-induced ulcerative colitis (UC) in Drosophila melanogaster were investigated. Our results demonstrated that treatment with 1% CLP80–1 significantly improved survival rates, attenuated gut atrophy, reduced intestinal permeability, enhanced intestinal barrier function, suppressed excessive proliferation of intestinal stem cells (ISCs) and enteroblasts (EBs), and inhibited the death of intestinal epithelial cells (IECs). Metabolomic and transcriptomic analyses revealed that CLP80–1 mainly affected amino acid metabolism, TCA cycle, oxidative phosphorylation and JAK/STAT signaling pathway. Furthermore, CLP80–1 reduced reactive oxygen species (ROS) levels, suppressed the expression of pro-inflammatory factors, and downregulated the JAK-STAT signaling pathway, thereby mitigating oxidative stress and inflammatory responses. These findings suggest that CLP80–1 is effective in alleviating intestinal inflammation and may serve as a promising therapeutic candidate for addressing gut inflammatory conditions.
✓ 已验证
产品关联
DOI
PubMed
高影响因子
RSC Advances
·
2026
·Vol. 16
·期 31
·28230-28242
·
IF 6.1
Jin-tuo Yin,De-mao Wang,Xue-chun Wu,Juan Lu,Zheng-ming Qian,De-qiang Li,Rui Feng
Taihangia rupestris Yu & Li (T. rupestris) was a nationally protected plant and its active compounds exhibit antidiabetic potential, but systematic phytochemical and pharmacological investigations remain limited. To balance conservation and utilization, the chemical composition and bioactivities (antioxidant and α-glucosidase inhibitory effects) of wild and cultivated T. rupestris were compared, followed by active compound screening. UPLC-MS/MS was used to identify chemical constituents in wild, mountain-cultivated, and foothill-cultivated samples, with multivariate analysis for differential components. The TFC and TPC, and the antioxidant capacity were evaluated via FRAP, CUPRAC, TRC, and DPPH assays. Additionally, α-glucosidase inhibition was assessed via IC50 determination. Online HPLC-ABTS and ultrafiltration-LC/MS (UF-LC/MS) were employed to screen antioxidants and α-glucosidase inhibitors (α-GIs), respectively, with molecular docking validating binding mechanisms. As a result, among 114 identified compounds, 111 showed significant environment-dependent variations, primarily flavonoids, phenolics, and terpenoids. Foothill-cultivated plants exhibited upregulated flavonoids/phenolics (e.g., rutin and gallic acid derivatives, P < 0.05) and superior antioxidant activity (Trolox equivalents: FRAP 367.18 ± 1.03; CUPRAC 572.40 ± 0.82) and α-glucosidase inhibition (IC50 0.2775 µg mL−1) versus wild (IC50 0.4948 µg mL−1) and mountain-cultivated samples (IC50 0.5425 µg mL−1). Ten antioxidants were screened, with seven also acting as α-GIs. UF-LC/MS and docking confirmed 8 α-GIs (binding energy < −5 kcal mol−1), where phenolic hydroxyl groups formed hydrogen bonds with ASP residues of α-glucosidase. Cultivated T. rupestris (especially foothill-grown) outperformed wild plants in bioactives composition and efficacy, serving as a sustainable alternative. Flavonoids and phenolics contributed to its antidiabetic potential via dual antioxidant and enzyme-inhibitory effects, supporting further pharmaceutical development.
✓ 已验证
DOI
PubMed
高影响因子
JOURNAL OF ALLOYS AND COMPOUNDS
·
2020
·
IF 4.65
Hailiang Li,Dechang Jia,Zhihua Yang,Yu Zhou
A facile heat treatment strategy suitable for TiB reinforced titanium matrix composites (TMCs) fabricated by selective laser melting (SLM) is proposed. The effects of boron modification and heat treatment on the microstructure and mechanical properties of the selective laser melted TMC samples were systematically investigated. The ultrafine ∼2 vol% TiB whiskers that formed in situ exhibited a much more homogeneous distribution in the matrix compared to that of conventionally processed composites containing similar volume fractions, further promoting the formation of fine equiaxed α-Ti grains throughout the matrix. An excellent combination of strength and fracture strain of 1428 MPa and 43.6%, respectively, was simultaneously achieved for the heat-treated TMC, in which the plasticity was improved by 126% over that of the as-fabricated composite (19.3%). This finding provides significant guidance for tailoring the microstructure in selective laser melted TMCs to achieve the desired mechanical properties.
✓ 已验证
DOI
高影响因子
NEOPLASIA
·
2026
·
IF 4.8
Hongming Teng,Yuxin Guo,Xinran Chen,Anlian Fan,Chengfei Zhang,Ting Zhang,Yuanyuan Luo,Lin Huang
Colorectal cancer (CRC) progression could be fueled by the activation of the unfolded protein response (UPR) triggered by endoplasmic reticulum (ER) stress. The proline-rich Akt1 substrate of 40 kDa (PRAS40) is implicated in cancer progression, but its role in the UPR remains unclear. Herein, we demonstrate that PRAS40 promotes the inositol-requiring enzyme 1α (IRE1α)-X-box binding protein 1 (XBP1) axis-dependent UPR in driving CRC progression. Mechanistically, PRAS40 interacts with ER chaperone glucose-regulated protein 78 (GRP78) and enhances its N-glycosylation. Moreover, PRAS40 improves the interaction between GRP78 and ST6 β-galactoside α-2, 6-sialyltransferase 1 (ST6Gal1), leading to increased α-2, 6-sialylation of GRP78 and the UPR triggered by ER stress. Furthermore, we identified the natural compound β-sitosterol as a novel ST6Gal1 inhibitor, which attenuated PRAS40-triggered tumor growth. Collectively, these findings unveil a PRAS40-ST6Gal1-GRP78 axis that drives CRC progression through activating the IRE1α-XBP-1-mediated UPR and nominate ST6Gal1 as a promising therapeutic target.
✓ 已验证
产品关联
DOI
高影响因子
MOLECULES
·
2026
·Vol. 31
·期 10
·1560
·
IF 5.1
Sisi Huang,Wei Cai,Yan Wang,Xiaoliang Xing,Zaiqi Zhang
Objective:This study aimed to investigate whether pomolic acid (PA), a predicted bioactive metabolite of the Dong botanical drug Madeng’ai (MDA), suppresses inflammatory cytokine expression by inhibiting nuclear factor-κB (NF-κB) pathway activation in a tumor necrosis factor-α (TNF-α)-induced human rheumatoid arthritis fibroblast-like synoviocyte (RA-HFLS) model.Methods:PA content in MDA from different regions and harvest years was quantified via High-Performance Liquid Chromatography (HPLC). Network analysis was employed as a hypothesis-generating tool to predict potential targets and pathways, followed by molecular docking to validate the binding affinity of PA to core targets of the NF-κB pathway, and ADMET prediction to evaluate its pharmacokinetic properties and safety profile. The RA-HFLS inflammatory model was induced by TNF-α. Cell viability and inflammatory cytokine secretion were assessed using Cell Counting Kit-8 (CCK-8) and enzyme-linked immunosorbent assay (ELISA). NF-κB signaling pathway activation and downstream gene expression were examined by Western blot and reverse transcription quantitative polymerase chain reaction (RT-qPCR), respectively.Results:HPLC analysis revealed that MDA samples from Guizhou harvested in 2019 contained the highest PA content (0.1117 mg/g). Network analysis predicted the NF-κB signaling pathway as a candidate mechanism underlying PA’s potential anti-inflammatory effects. Molecular docking showed that PA stably bound to IKKβ, p65, and IκBα, while ADMET prediction indicated favorable intestinal absorption, low drug–drug interaction risk, and good genetic safety, albeit with potential hepatotoxicity and reproductive toxicity risks. In the TNF-α-induced RA-HFLS model, PA dose-dependently inhibited abnormal cell proliferation and significantly reduced the secretion of pro-inflammatory cytokines TNF-α and interleukin-6 (IL-6). Mechanistic studies indicated that PA suppressed the activation of the NF-κB signaling pathway, thereby downregulating the mRNA expression of inflammatory genes such as IL-6, TNF-α, and interleukin-1β (IL-1β).Conclusions:PA, a bioactive metabolite of the Dong botanical drug MDA, may inhibit NF-κB signaling pathway activation, thereby downregulating TNF-α-induced inflammatory cytokine expression in RA-HFLS, demonstrating its in vitro anti-inflammatory potential.
✓ 已验证
DOI
PubMed
高影响因子
FOOD CHEMISTRY
·
2026
·
IF 10.4
Caiyun Liu,Zengshuai Zhang,Juan Jiang,Mario Prejanò,Tiziana Marino,Nino Russo,Yongsheng Tao,Yunkui Li
α-CD significantly enhances the extraction of phenolics during wine fermentation. • α-CD promotes accumulation of key volatile esters, enhancing fruity and floral aroma. • α-CD is associated with amino acid metabolism changes linked to aroma formation. • α-CD-induced volatile enrichment strongly correlate with enhanced sensory perception. • α-CD offers a novel dual-function strategy to enhance wine aroma and sensory quality. This study investigated the potential mechanisms by which α-CD enhances phenolic extraction, modulates aroma compound accumulation, and improves sensory quality during wine fermentation. The addition of α-CD significantly increased phenolic extraction and promoted the accumulation of several key aroma-active volatiles. Sensory evaluation indicated that wines treated with α-CD exhibited enhanced fruity, floral, and berry aroma attributes compared with the control. PLSR analysis showed that multiple high-OAV volatiles were associated with fruity, floral, sweet, green, sour-fruit, and berry sensory attributes, indicating clear relationships between chemical composition and sensory perception. Untargeted metabolomic analysis further suggested that α-CD treatment was associated with changes in amino acid-related metabolism and aroma precursor availability, which may contribute to the accumulation of fermentation-derived aroma compounds. Collectively, these findings suggest that α-CD may improve phenolic content, aroma complexity, and sensory quality through complementary effects involving phenolic extraction, selected volatile retention, and fermentation-associated metabolic changes. Download: Download high-res image (229KB) Download: Download full-size image
✓ 已验证
DOI
高影响因子
JOURNAL OF CEREAL SCIENCE
·
2022
·
IF 4.075
Yake Xiang,Xiao Chen,Hailan Sun,Qiping Zhan,Lei Zhong,Qiuhui Hu,Liyan Zhao
To preserve the nutrients while maximizing the value of black waxy corn , beverage is the most promising manufacturing product. However, the poor stability and bland flavor profile are significant issues limiting its popularization. This study, firstly profiled the anthocyanidins of black waxy corn and the effects of α-amylase and amyloglucosidase treatments on the anthocyanidin levels, color properties, and flavor qualities were systematically studied. A total of 5 anthocyanidins and 11 anthocyanins were identified. The concentration of cyanidin , petunidin , peonidin , pelargonidin , and malvidin in liquefied liquids (YHY) and saccharified liquids (THY) were significantly increased by 55.46% and 56.62% after α-amylase and amyloglucosidase treatments. The enzymatic hydrolysis was found to enrich the volatile profile. The relative odor activity value and multivariate statistical analyses further suggested isoamyl acetate , benzaldehyde , 2-nonenal, 2,4-decadienal, dodecanal , 2-buten-1-one, 3-buten-2-one, and dimethyl trisulfide as key odorants to discriminate black waxy corn beverages produced by α-amylase or amyloglucosidase.
✓ 已验证
产品关联
DOI
高影响因子
Food & Function
·
2023
·Vol. 14
·期 4
·1952-1961
·
IF 6.1
Jinfang Xu,Zhiyu Fan,Gangqiang Yang,Yanan Yang,Xinjie Wu,Tongtong Li,Qili Wang,Jianping Gao
In this study, a ligand fishing technique based on magnetic mesoporous silicon was established and used to screen α-glucosidase inhibitors from Pueraria lobata. To clarify quantity–activity relationships in a holistic view, the knock-out/knock-in technology was used to analyse the interactions of several active constituents in P. lobata. Magnetic mesoporous silicon with a large specific surface area and better biocompatibility was synthesised. Subsequently, α-glucosidase was immobilised on –NH2-modified magnetic mesoporous silicon, and the compounds in the crude extract of P. lobata were screened across enzyme binding. The structures of the ligands were elucidated using UPLC-Q-TOF-MS/MS, and their activities were verified by knock-out/knock-in experiments and molecular docking. Daidzein and puerarin showed α-glucosidase inhibitory activities with an IC50 of 0.088 ± 0.003 mg mL−1 and 0.414 ± 0.005 mg mL−1, respectively. Among them, puerarin, which accounted for more than 40% of the total content, showed synergistic effects with other components and was the main contributor to the α-glucosidase inhibitory activity of P. lobata.
✓ 已验证
产品关联
DOI
PubMed
高影响因子
CHEMICAL ENGINEERING JOURNAL
·
2023
·
IF 15.1
Liqin Liu,Xingye An,Xiaohong Zhang,Zhengbai Cheng,Jinhuan Guo,Shuangxi Nie,Haibing Cao,Hongbin Liu,Yonghao Ni
Recently starch based bio-latexes are facing serious bottlenecks in their preparation and applications especially at the aspects of high concentration, high colloidal stability and high rheological performance. Starch paste for bio-latex production at high concentration would be prone to retrogradation and inevitably deteriorate its performance. The excessive and uncontrollable α-amylase hydrolysis behavior of starch granules at low concentration below 30 wt% could severely affect the strengthening or binding ability of bio-latex. Herein, this study proposes a practical and effective strategy by using cationic cellulose nanocrystals (CCNC) as versatile carriers of α-amylase for the preparation of starch based bio-latex (SBL) to improve starch hydrolysis efficiency even at 50 wt% concentration. CCNC immobilized α-amylase (CCNC/E) can effectively regulate starch hydrolysis behavior with controllable amylose/ amylopectin molecular weight/ distribution for enhanced colloidal stability and viscoelasticity. Furthermore, the multifunctional roles of CCNC as excellent dispersants, nano-fillers and enhancers within starch colloids could impart SBL excellent rheological property and printability in pigmented coating and printing performance, by improving the coating surface, higher printing density/ picking velocity, and lower dot gain phenomenon, compared with the control latex samples. This study proposes a promising and practical approach for efficient production of 50 wt% starch based bio-latex with advanced performance and further valorizes the cellulose nanocrystals in the starch based biomaterials applications.
✓ 已验证
产品关联
DOI
高影响因子
CARBOHYDRATE POLYMERS
·
2023
·
IF 11.2
Chunwang Hu,Hongliang Wei,Bingyan Hua,Yaqi Zhang,Gang Wang,Tao Guo
A thermal/pH-sensitive interpenetrating network (IPN) hydrogel was prepared facilely from starch and poly(α- l -lysine) through amino-anhydride and azide-alkyne double-click reactions in one pot. The synthesized polymers and hydrogels were systematically characterized using different analytical techniques such as Fourier transform infrared spectroscopy (FTIR), nuclear magnetic resonance (NMR), scanning electron microscope (SEM), X-ray diffraction (XRD), X-ray photoelectron spectroscopy (XPS), and rheometer. The preparation conditions of the IPN hydrogel were optimized via one-factor experiments. Experimental results indicated the IPN hydrogel possessed pH and temperature sensitivity. Effect of different parameters (pH, contact time, adsorbent dosage, initial concentration, ionic strength , and temperature) on adsorption behavior were investigated in monocomponent system with cationic methylene blue (MB) and anionic Eosin Y (EY) as model pollutants. The results indicated that the adsorption process of the IPN hydrogel for MB and EY followed pseudo-second-order kinetics. The adsorption data for MB and EY fitted well with the Langmuir isotherm model, indicating monolayer chemisorption. The good adsorption performance was due to various active functional groups (–COOH, –OH, –NH 2 , etc.) in the IPN hydrogel. The strategy described here opens up a new way for preparing IPN hydrogel. The as-prepared hydrogel exhibits potential application and bright prospects as an adsorbent in wastewater treatment.
✓ 已验证
产品关联
DOI
PubMed
高影响因子
FOOD RESEARCH INTERNATIONAL
·
2022
·
IF 7.425
Chao Qi,Yueying Jin,Shengyu Cheng,Lei Di,Xin Wang,Minghui Zhang,Lingli Zhang,Xi-Ling Li,Yu Han,Qingkun Ma,Jun Zhe Min
α-dicarbonyl compounds (α-DCs) serve as potential biomarkers for oxidative stress-related diseases but are difficult to detect. To study the metabolism of carbonyl compounds , we developed a new mass spectrometry probe, 3-benzyl-2-oxo-4λ 3 -thiazolidine-4-carbohydrazide (BOTC), containing hydrazyl groups for the targeted detection of carbonyl functional groups. In a novel approach, we used BOTC pre-column derivatization with ultrahigh performance liquid chromatography–tandem mass spectrometry (UHPLC-MS/MS) to simultaneously detect four kinds of α-DCs in red wine as well as in urine after drinking. The α-DCs were completely separated (R 2 ≥ 0.9995), detection was sensitive (detection limit was 12.5–50 fmol), consistent (intraday and interday precision was 0.1–5.7 %), and efficient (average recoveries were 103.3–110.2 %). The method was applied to the analysis of α-DCs in different wines and the dynamic monitoring of transit and excretion in vivo after drinking. Our novel method provides a new strategy for the detection of α-dicarbonyl compounds in red wine and dicarbonyl compounds produced in oxidative stress-related diseases.
✓ 已验证
产品关联
DOI
PubMed
高影响因子
危险品化学品经营许可证（带存储）