Plasmonics
·
2020
·Vol. 15
·期 6
·1827-1833
·
IF 2.335
Wang Junren,Li Hua,Yu Haili,He Yi
Considerable efforts have been made to develop reactive oxygen species (ROS) scavengers for removing high level of ROS. However, most of the reported ROS scavengers are nondegradable and involve harsh reaction conditions as well as utilize various surface ligands. In order to overcome these drawbacks, in the present work, we develop a facile and mild synthesis avenue for preparation of surface-clean hydrogen-doped molybdenum oxide (H 0.34 MoO 3 ) via simply mixing MoO 3 dispersion with aluminum foil under an acidic environment without any surface capping reagents at room temperature. The resulting H 0.34 MoO 3 can act as a broad-spectrum ROS scavenger, including . OH, H 2 O 2 , O 2 − , and 1 O 2 as well as 2, 2-diphenyl-1-picrylhydrazyl (DPPH). The free radical scavenging activity of H 0.34 MoO 3 achieves as high as 71.6% and 99.1% for . OH and DPPH scavenging, which is comparable and superior to that of ascorbic acid that is a classic free radical scavenger. More significantly, the resulting H 0.34 MoO 3 is degrade, which can be degraded into molybdate ions under a neutral environment (pH 7.4).
✓ 已验证
DOI
高影响因子
MICROCHIMICA ACTA
·
2022
·Vol. 189
·期 9
·1-11
·
IF 6.408
Zhai Hongguo,Wang Yue,Yin Jiaqi,Zhang Yuhao,Guo Qi,Sun Xia,Guo Yemin,Yang Qingqing,Li Falan,Zhang Yanyan
MXene@Au as the base and Au@SiO 2 as signal amplification factor were used for constructing an ultrasensitive “on–off” electrochemiluminescence (ECL) biosensor for the detection of Pb 2+ in water. The use of MXene@Au composite provided a good interface environment for the loading of tris(2,2-bipyridyl)ruthenium(II) (Ru(bpy) 3 2+ ) on the electrode. Based on resonance energy transfer, the Au (core) SiO 2 (shell) (Au@SiO 2 ) nanoparticles stimulate electron transport and promote tripropylamine (TPrA) oxidation. The luminescence effect of Au@SiO 2 was five times that of AuNPs and SiO 2 nanomaterials alone, and the ECL intensity was greatly improved. In addition, Pb 2+ activated the aptamer to exert its endonuclease activity, which realized the signal cycle amplification in the process of Pb 2+ detection. When Pb 2+ was added, the ECL signal weakened, and the Pb 2+ concentration was detected according to the decreased ECL intensity. Under optimized experimental conditions, this aptamer sensor for Pb 2+ has a wide detection range (0.1 to 1 × 10 6 ng L −1 ) and a low detection limit (0.059 ng L −1 ). The relative standard deviation (RSD) of the sensor is 0.39–0.99%, and the recovery of spiked standard is between 90.00 and 125.70%. The sensor shows good selectivity and high sensitivity in actual water sample analysis. This signal amplification strategy possibly provides a new method for the detection of other heavy metal ions and small molecules. Graphical abstract
✓ 已验证
DOI
PubMed
高影响因子
MICROCHIMICA ACTA
·
2022
·Vol. 189
·期 8
·1-10
·
IF 6.408
Zhao Tingting,Yan Weizhen,Dong Fengqi,Hu Xinlong,Xu Yanli,Wang Zhenyu,Shen Yating,Wang Wanrong,Zhao Ye,Wei Wenmei
COVID-19 necessitates the development of reliable and convenient diagnostic tools. In this work, a facile 3D-printed smartphone platform was constructed that achieved reliable visual detection of SARS-CoV-2 by eliminating the effect of ambient light and fixing the camera position relative to the sample. The oligonucleotide probe is modified with orange-red-emitting TAMRA working as an internal standard and green-emitting FAM serving as a sensitive sensing agent. Under 365-nm UV excitation, the emission wavelengths of TAMRA and FAM are 580 nm and 518 nm, respectively. When the probes interact with the targets, the green fluorescence gradually restores while the orange-red fluorescence remains stable. Thus, a striking color transition from orange-red to green could be observed by the naked eye. The detection limit of SARS-CoV-2 nucleic acid is 0.23 nM, and the entire process of color change could be completed in 25 min. Furthermore, the RGB value analysis of the sample solution was conducted using a smartphone for reliable and reproducible discrimination of SARS-CoV-2. The proposed smartphone platform might establish a general method for visual detection of SARS-CoV-2 nucleic acid as well as other virus-related diseases. Graphical abstract
✓ 已验证
DOI
PubMed
高影响因子
BMC MICROBIOLOGY
·
2022
·Vol. 22
·期 1
·1-16
·
IF 4.465
Wu Xinying,Cai Wen,Zhu Pengcheng,Peng Zheng,Zheng Tianfei,Li Dongliang,Li Jianghua,Zhou Guanyu,Du Guocheng,Zhang Juan
Background The aging process in the tobacco production, as in other food industries, is an important process for improving the quality of raw materials. In the spontaneous aging, the complex components in flue-cured tobacco (FT) improve flavor or reduce harmful compounds through chemical reactions, microbial metabolism, and enzymatic catalysis. Some believed that tobacco-microbe played a significant part in this process. However, little information is available on how microbes mediate chemical composition to improve the quality of FT, which will lay the foundation for the time-consuming spontaneous aging to seek ways to shorten the aging cycle. Results Comparing aged and unaged FT, volatile and non-volatile differential compounds (DCs) were multi-dimensionally analyzed with the non-targeted metabolomes based on UPLC-QTOP-MS (the ultra-performance liquid chromatography quadrupole time-of-flight mass spectrometry), GC–MS (gas chromatography-mass spectrometer) assisted derivatization and HP-SPME-GC/MS (headspace solid-phase micro-extraction assisted GC–MS). Products associated with the degradation pathways of terpenoids or higher fatty acids were one of the most important factors in improving FT quality. With the microbiome, the diversity and functions of microbial flora were analyzed. The high relative abundance function categories were in coincidence with DCs-related metabolic pathways. According to the correlation analysis, Acinetobacter , Sphingomonas and Aspergillus were presumed to be the important contributor, in which Aspergillus was associated with the highest number of degradation products of terpenoids and higher fatty acids. At last, the screened Aspergillus nidulans strain F4 could promote the degradation of terpenoids and higher fatty acids to enhance tobacco flavor by secreting highly active lipoxygenase and peroxidase, which verified the effect of tobacco-microbes on FT quality. Conclusions By integrating the microbiome and metabolome, tobacco-microbe can mediate flavor-related substances to improve the quality of FT after aging, which provided a basis for identifying functional microorganisms for reforming the traditional spontaneous aging.
✓ 已验证
DOI
PubMed
高影响因子
MICROCHIMICA ACTA
·
2018
·Vol. 185
·期 7
·1-8
·
IF 5.705
He Baoshan
Graphene oxide doped with nitrogen and sulfur was decorated with gold nanoparticles (AuNP-SN-GO) and applied as a substrate to modify a glassy carbon electrode (GCE). An aptamer against the model protein thrombin was self-assembled on the modified GCE which then was exposed to thrombin. Following aptamer-thrombin interaction, biotin-labeled DNA and aptamer 2 are immobilized on another AuNP-SN-GO hybrid and then are reacted with the thrombin/AuNP-SN-GO/GCE to form a sandwich. The enzyme label horseradish peroxidase (HRP) was then attached to the electrode by biotin–avidin interaction. HRP catalyzes the oxidation of hydroquinone by hydrogen peroxide. This generates a strong electrochemical signal that increases linearly with the logarithm of thrombin concentration in the range from 1.0 × 10 −13 M to 1.0 × 10 −8 M with a detection limit of 2.5 × 10 −14 M (S/ N = 3). The assay is highly selective. It provides a promising strategy for signal amplification. In our perception, it has a large potential for sensitive and selective detection of analytes for which appropriate aptamers are available.
A sandwich-type electrochemical aptasensor is fabricated for detection of thrombin using a glassy carbon electrode modified with nitrogen- and sulfur-doped graphene oxide and gold nanoparticles.
✓ 已验证
DOI
PubMed
高影响因子
Frontiers of Medicine
·
2021
·Vol. 15
·期 6
·887-902
·
IF 4.592
Wu Xin,Wang Zongqi,Li Haiying,Xie Xueshun,Wu Jiang,Shen Haitao,Li Xiang,Wang Zhong,Chen Gang
Monocarboxylic acid transporter 1 (MCT1) maintains axonal function by transferring lactic acid from oligodendrocytes to axons. Subarachnoid hemorrhage (SAH) induces white matter injury, but the involvement of MCT1 is unclear. In this study, the SAH model of adult male Sprague-Dawley rats was used to explore the role of MCT1 in white matter injury after SAH. At 48 h after SAH, oligodendrocyte MCT1 was significantly reduced, and the exogenous overexpression of MCT1 significantly improved white matter integrity and long-term cognitive function. Motor training after SAH significantly increased the number of ITPR2 + SOX10 + oligodendrocytes and upregulated the level of MCT1, which was positively correlated with the behavioral ability of rats. In addition, miR-29b and miR-124 levels were significantly increased in SAH rats compared with non-SAH rats. Further intervention experiments showed that miR-29b and miR-124 could negatively regulate the level of MCT1. This study confirmed that the loss of MCT1 may be one of the mechanisms of white matter damage after SAH and may be caused by the negative regulation of miR-29b and miR-124. MCT1 may be involved in the neurological improvement of rehabilitation training after SAH.
✓ 已验证
DOI
PubMed
高影响因子
Communications Earth & Environment
·
2026
·
IF 9.3
Wang Xuemeng,Li Lingyan,Li Huiju,Li Jie,Thies Janice,Xue Kai,Dong Xiuzhu
Wetlands are a significant source of methane, a potent greenhouse gas that contributes to global warming. Acetate and H 2 /CO 2 are generally considered the dominant precursors for methane formation, while H 2 , generally considered as the electron donor, is considerably lower in wetlands. This work investigated alternative reductive mechanisms of methanogenesis in the soil microcosms of Zoige wetland on Tibetan Plateau. Carbon monoxide inhibition of hydrogenase combined with isotope tracing experiments indicated only 2.9%-12.5% of the wetland H 2 was used in methanogenesis. Electron shuttles (biochar and magnetite) promoted methane production, and electrochemical experiments detected current generation from bacteria and current consumption coupled to methane production, confirming that electromethanogenesis occurred in the Zoige wetland and contributed 27.7%-50.9% of CH 4 production. Moreover, electromethanogenesis exhibited 2.6-16.1-fold higher CO 2 - and methanol-reductive methanogenic efficiencies than using H 2 . Uncultured Rice Cluster Ⅱ methanogens and a family of H 2 -dependent methylotrophic methanogen called Methanomassiliicoccaceae were enriched in electrochemical microcosms. Remarkably, oxidized anthraquinone-2,6-disulfonate decreased methane production. This work verifies the role of electromethanogenesis in a Tibetan wetland and provides a potential strategy for reducing methane emission.
✓ 已验证
DOI
高影响因子
Science China-Materials
·
2026
·
IF 7.7
Zhang Jianhao,Jia Yi,Li Quan,Wang Yang,Pan Limei,Yang Jian
Development of flexible materials integrating broadband electromagnetic wave (EMW) absorption, infrared stealth, and hydrophobicity is required for complex environments. In this work, flexible SiTiOC ceramic fiber felts embedded with Sn nanoparticles were fabricated via electrospinning followed by in-situ carbothermal reduction. The metallic Sn promotes carbon graphitization, forming a three-dimensional conductive network and establishing heterogeneous interfaces within the SiTiOC matrix. First-principles calculations indicate that the work function gradient ( Φ Sn < Φ C ) drives electron migration at the Sn/C interface, inducing interfacial polarization, while turbostratic carbon in the SiTiOC phase provides defect dipole polarization. The fiber felt exhibits an effective absorption bandwidth of 7.3 GHz at a thickness of 3.75 mm. The material integrates radar-infrared compatible stealth through low thermal conductivity and low emissivity, with a water contact angle exceeding 138°. These results provide an interface engineering strategy for multifunctional stealth materials in complex environments.
✓ 已验证
DOI
高影响因子
npj Vaccines
·
2026
·
IF 7.2
Yang Wenqi,Zhao Qingqing,Shi Wendi,Yan Jiacheng,Tang Di,Hu Yu,Zhang Linbo,Song Yueqi,Zhou Rui,Ren Qian,Li Yanyi,Wang Xintong,Cao Yufeng,Shi Li,Liang Zhihui,Xue Changying,Sun Bingbing
As a critical advancement in modern immunization strategies, multivalent vaccines combine multiple antigenic components to seamlessly broaden protection against diverse pathogens within a single dose. During the formulation of aluminum-adjuvanted vaccines, antigen adsorption onto the adjuvant surface serves as a critical determinant of vaccine performance. However, it remains unclear how the sequence of adsorption during formulation influences the particle-to-particle antigen loading heterogeneity on adjuvant particles and whether such differences are associated with immune outcomes. In this study, we systematically investigate how the adsorption sequence modulates particle-to-particle antigen-loading heterogeneity and antibody binding in bivalent vaccines. Using human papillomavirus (HPV16/18) and norovirus (NoV GI.1/GII.4) virus-like particles (VLPs) as model antigens with different adsorption strengths for amorphous aluminum hydroxyphosphate sulfate (AAHS), we demonstrate that the strength of antigen–adjuvant interaction is associated with how adsorption sequence influences antigen distribution. Antigens with weaker binding affinity exhibit distinct particle-to-particle antigen loading heterogeneity depending on the formulation sequence, whereas those with strong interactions remain largely unaffected. Importantly, these variations in particle-to-particle antigen-loading heterogeneity were associated with differences in antibody and monoclonal antibody binding, and with differences in in vivo humoral immune responses. These findings highlight adsorption sequence as an important formulation parameter associated with antigen distribution and antibody responses in multivalent vaccines.
✓ 已验证
DOI
高影响因子
CHINESE JOURNAL OF POLYMER SCIENCE
·
2019
·Vol. 38
·期 4
·342-348
·
IF 2.804
Yang Ying,Shan Tong,Cao Jian,Wang Hua-Chun,Wang Ji-Kang,Zhong Hong-Liang,Xu Yun-Xiang
Two conjugated polymers (PuIDTBD and PuIDTQ) with unsymmetric side chains have been prepared for polymer solar cells using two other polymers (PIDTBD and PIDTQ) with symmetric side chains as control compounds. The combination of methyl and 4-hexylphenyl side chains on the same bridged carbon can ensure good solubility, decrease π - π stacking distances, and bring proper miscibility with PC 71 BM simultaneously. Therefore, the corresponding polymer solar cells (PSCs) based on donor polymers with unsymmetric side chains exhibited enhanced short-circuit current density ( J SC ) and power conversion efficiency (PCE) compared with those of control polymers. The PIDTBD and PIDTQ based devices possessed low PCE of 2.13% and 1.48%, while PCEs of devices based on PuIDTBD and PuIDTQ were improved to 3.93% and 4.12%, respectively. The results demonstrate that unsymmetric side chain engineering of conjugated polymers is an effective approach to achieve high performance PSCs.
✓ 已验证
DOI
高影响因子
ANALYTICAL AND BIOANALYTICAL CHEMISTRY
·
2022
·Vol. 414
·期 26
·7675-7681
·
IF 4.478
Sheng Yilun,Huang Zhongnan,Chen Yaling,Guo Xiaoyun,Chen Lan,Peng Huaping,Chen Wei
Monitoring of dihydropyridine drugs, such as nifedipine (NIF), has attracted considerable attention owing to the side effects arising from the consumption of such drugs. Herein, a highly sensitive and facile fluorescence-sensing platform based on a high-quantum-yield sulfur quantum dot (SQDs) probe for NIF detection is proposed. Based on the principle of the inner filter effect, the rapid detection of NIF with high sensitivity is successfully realized on the basis of the change in the fluorescence signal due to the quenching effect of NIF on SQDs. The results show a good linear relationship between the NIF concentration and fluorescence intensity within the range of 5–150 μmol/L, with a low detection limit of 1.63 μmol/L (S/N = 3). Moreover, because no surface modification or establishment of any coupling between the receptor and the fluorophore is necessary, this approach provides considerable flexibility and simplicity for the construction of a fluorescence sensor and substantially reduces the detection time. A systematic investigation was conducted to verify the applicability of this method for the analysis of pharmaceutical components in NIF tablets. This study not only promotes the design and development of a fluorescence analysis platform for NIF detection, but also facilitates the fabrication of novel SQD-based fluorescence-sensing systems for the molecular detection of drugs. Graphical abstract
Proposal for a facile nifedipine assay method based on the inner filter effect of nifedipine to high-quantum-yield sulfur quantum dots, and realizing nifedipine detection in tablets and human urine samples.
✓ 已验证
DOI
PubMed
高影响因子
Frontiers of Chemical Science and Engineering
·
2023
·Vol. 17
·期 7
·906-917
·
IF 4.5
Zou Min,Tan Chao,Yuan Zhengqiu,Wu Ming,Jian Jian,Zhang Lei,Zhang Yan,Ma Zhou,Zhou Hu
The industrial application of nano-photocatalysts in wastewater treatment has been severely restricted for a long time due to their difficult separation, poor reusability, and low efficiency. In this work, a facile strategy was proposed to enhance the photocatalytic activity and recovery performance of Ag@AgCl nanocatalysts. Biological veins (Bio-veins) with a unique 3D porous construction were used as carriers for the in-situ growth of Ag@AgCl nanoparticles. Scanning electron microscopy results showed that the Ag@AgCl nanoparticles were uniformly loaded on the surface and interior of the Bio-veins, and the size of the Ag@AgCl nanoparticles immobilized on the Bio-veins (50–300 nm) was significantly smaller than Ag@AgCl obtained by the co-precipitation method (1–3 µm). The Bio-veins played a vital role in the photocatalysis reaction system. The degradation efficiency of the Ag@AgCl/Bio-veins(CI4) was up to 3.50 times as high as pure Ag@AgCl. Furthermore, the composites also exhibited excellent recyclability and stability under both visible and solar light. This work provided a suitable strategy for nanophotocatalysts for practical application and may also offer new possibilities for the high-value utilization of biomass materials.
✓ 已验证
DOI
高影响因子
危险品化学品经营许可证（带存储）