查找使用阿拉丁产品的科研文献

A Bruton tyrosine kinase inhibitor-resistance gene signature predicts prognosis and identifies TRIP13 as a potential therapeutic target in diffuse large B-cell lymphoma

Ding Yangyang,Huang Keke,Sun Cheng,Liu Zelin,Zhu Jinli,Jiao Xunyi,Liao Ya,Feng Xiangjiang,Guo Jingjing,Zhu Chunhua,Zhai Zhimin,Xiong Shudao

Bruton tyrosine kinase inhibitor (BTKi) combined with rituximab-based chemotherapy benefits diffuse large B-cell lymphoma (DLBCL) patients. However, drug resistance is the major cause of relapse and death of DLBCL. In this study, we conducted a comprehensive analysis BTKi-resistance related genes (BRRGs) and established a 10-gene (CARD16, TRIP13, PSRC1, CASP1, PLBD1, CARD6, CAPG, CACNA1A, CDH15, and NDUFA4) signature for early identifying high-risk DLBCL patients. The resistance scores based on the BRRGs signature were associated with prognosis. Furthermore, we developed a nomogram incorporating the BRRGs signature, which demonstrated excellent performance in predicting the prognosis of DLBCL patients. Notably, tumor immune microenvironment, biological pathways, and chemotherapy sensitivity were different between high- and low-resistance score groups. Additionally, we identified TRIP13 as a key gene in our model. TRIP13 was found to be overexpressed in DLBCL and BTKi-resistant DLBCL cell lines, knocking down TRIP13 suppresses cell proliferation, promotes cell apoptosis, and enhances the apoptosis effect of BTKi on DLBCL cells by regulating the Wnt/β-catenin pathway. In conclusion, our study presents a novel BRRGs signature that could serve as a promising prognostic marker in DLBCL, and TRIP13 might be a potential therapeutic target for resistant DLBCL.

Ionic-liquid-based ultrasound-assisted extraction combined with countercurrent chromatography and semipreparative LC for the preparation of monoamine oxidase B inhibitors from Pueraria thomsonii

Sainan Li,Yanjie Li,Ying Wang,Ruizhe Li,Huazhou Niu,Chunming Liu,Yuchi Zhang

A simple and efficient method was developed for the rapid screening and identification of ligands for monoamine oxidase B. A new ionic-liquid-based ultrasound-assisted extraction method for medicinal herbs was also developed and validated. In addition, the hyphenated technique of countercurrent chromatography and semipreparative-LC was developed and applied to the isolation of the chemical constituents for Pueraria thomsonii Benth. Three potent monoamine oxidase B inhibitors, namely, daidzein-4′,7-diglucoside (42.2 mg), puerarin 6′′- O -xyloside (88.3 mg), and 3′-hydroxypuerarin (48.5 mg) with purities of 98.2, 96.3, and 97.1%, respectively, were obtained from 500 g of P. thomsonii raw material using semi-preparative high-performance liquid chromatography, whereas 3′-methoxypuerarin (76.2 mg), daidzein-8-C-apiosyl (1→6) glucoside (84.2 mg), and tectorigenin (75.1 mg) with purities of 98.5, 96.4, and 96.8%, respectively, were obtained from 500 g raw material via countercurrent chromatography using a two-phase solvent system comprising n -hexane–ethyl acetate–methanol–water at a volume ratio of 1.85:1.00:0.86:3.69 (v/v/v/v). Then, the anti-Alzheimer activity of the phytochemicals was assessed using a PC12 cell model. Treatment with tectorigenin, daidzein-4′,7-diglucoside, puerarin 6′′- O -xyloside, 3′-hydroxypuerarin, 3′-methoxypuerarin, and daidzein-8-C-apiosyl (1→6) glucoside (100 μg/mL), resulted in cell viabilities of 69.00, 65.81, 59.69, 57.90, 55.61, and 54.59%, respectively ( p < 0.001). The protocol was proved to be very accurate and efficient.

Chinas Unlimited: Making the Imaginaries of China and Chineseness. By Gregory B. Lee. Honolulu: University of Hawai'i Press, 2003. xi, 121 pp. $45.00 (cloth).

Kathleen Erwin

Research on the Synergistic Enhancement of Corrosion Resistance and Wear Resistance of Ni–W–B-Based Coatings by Nano CeO2 and PDA Functionalized VC

Xianmin Gong,Shijun Xu,Yiling He,Kaijun Wei,Quangang Chen,Qing Yuan,Yi He,Yi Fan,Xingtao Cheng

Metal-based composite coatings have been widely studied for their excellent properties. In order to improve the properties of nickel tungsten boron (Ni–W–B) matrix, Ni–W–B/PC coatings were prepared by electrodeposition in the presence of polydopamine functionalized VC (P–VC) and cerium oxide (CeO2) nanoparticles. The effect of cerium oxide content in the nanomaterials on the properties of Ni–W–B coatings was investigated. The results showed that the prepared coatings were dense, uniform, and crack-free, and the best performance was achieved at a cerium oxide content of 2 g/L. The addition of CeO2 nanoparticles can fill the tiny defects in the coating and optimize the coating structure. The addition of these two materials refines the size of the coating grains. In terms of coating performance, the corrosion resistance of the composite coating was enhanced with the addition of CeO2 at a certain P–VC concentration, and the total impedance of the composite coating was 24,030 Ω·cm2, which was 4.8 times higher than that of the Ni–W–B coating and 1.7 times higher than that of the Ni–W–B/P–VC coating. In addition, the hardness of the Ni–W–B/PC-2 composite coating reached 1033.93 HV at a CeO2 content of 2 g/L. The wear resistance of this composite coating was also optimized. Compared with the Ni–W–B/P–VC composite coating, the average coefficient of friction was reduced by 8.8%, and the wear resistance of the composite coating was further improved, which was attributed to the synergistic effect of the two materials. Moreover, the Ni–W–B/PC film has excellent hardness and corrosion resistance, which is promising for the application in harsh environments.

CircRNA CTNNB1 (circCTNNB1) ameliorates cerebral ischemia/reperfusion injury by sponging miR-96-5p to up-regulate scavenger receptor class B type 1 (SRB1) expression

Chun Chen,Xiaolong Chang,Shifei Zhang,Qi Zhao,Chunyan Lei

Emerging studies show that circRNA catenin beta 1 (circCTNNB1) plays a critical role in cancer. However, the expression and function of circCTNNB1 in cerebral ischemia/reperfusion injury (IRI) have not been reported. The present study discovered that circCTNNB1 and scavenger receptor class B type 1 (SRB1) expression levels were significantly down-regulated in mouse astrocytes (mAS) treated with oxygen glucose deprivation and reperfusion (OGD/R), and similar results were observed in a mouse middle cerebral artery occlusion model. Overexpression of circCTNNB1 alleviated cell apoptosis, oxidative stress and the inflammatory response induced by OGD/R in vitro. Up-regulation of circCTNNB1 increased SRB1 expression levels to protect mAS cells from OGD/R-induced damage. CircCTNNB1 and SRB1 interacted with miR-96-5p, and the overexpression of miR-96-5p efficiently reversed the function of circCTNNB1 in OGD/R-treated mAS cells. CircCTNNB1 protected against cerebral ischemia-reperfusion injury by up-regulating SRB1 in vivo. In conclusion, our findings suggest that circCTNNB1 acts as a competitive endogenous RNA for miR-96-5p to alleviate cerebral IRI, which provides novel evidence that circCTNNB1 and SRB1 may be biomarkers and therapeutic targets for cerebral IRI.

Ban+2Znn(BO3)n(B2O5)Fn (n = 1, 2): new members of the zincoborate fluoride series with two kinds of isolated B–O units

Miriding Mutailipu,Xin Su,Min Zhang,Zhihua Yang,Shilie Pan

Two new members of zincoborate fluoride series Ban+2Znn(BO3)n(B2O5)Fn (n = 1, 2), namely, Ba3Zn(BO3)(B2O5)F and Ba4Zn2(BO3)2(B2O5)F2 were firstly synthesized as high-quality single crystals via a high-temperature solution method by adjusting the molar ratios of reactants. They were structurally determined by single-crystal X-ray diffraction and their structures can be regarded as three dimensional (3D) framework stacking of 2∞[Zn2B6O16]10− layers for Ba3Zn(BO3)(B2O5)F and 2∞[Zn2B4O8O5/2O1/3]17/3− layers for Ba4Zn2(BO3)2(B2O5)F2. To the best of our knowledge, the two title compounds are the first examples of two kinds of isolated B–O groups that coexist in one zincoborate halide. The structure comparisons were carried out among all the available anhydrous-zincoborates, and it is interesting to conclude that about 80% of zincoborates contain isolated B–O clusters and notably the molar ratio of cation/boron (A/B) affects the polymerization of isolated B–O configuration. What's more, the first-principles calculations, infrared spectra, and UV-vis-NIR diffuse reflectance spectra of title compounds have also been performed.

Two T-Shaped Donor–Acceptor Small Molecules Based on 4,9-Di(thiophen-2-yl)naphtho[2,3-b]thiophene for Solution-Processed Organic Solar Cells

Hua Tan,Wenhong Peng,Hongjian Liu,Yuhong Luo,Yu Chen,Linrui Duan,Junting Yu,Youming Zhang,Jun Zhang,Yafei Wang,Renqiang Yang,Weiguo Zhu

Two novel T-shaped π-conjugated small molecules of DTNT-3DPP and DTNT(DPP-PN) 3 with 4,9-di(thiophen-2-yl)naphtho[2,3- b ]thiophene (DTNT) as the donor core and diketopyrrolopyrrole (DPP) as acceptor arms, in the latter case incorporating phenanthrene (PN) units, have been designed and synthesized. The effects of the central core and terminal units on the optical, thermal, electrochemical, and photovoltaic properties have been investigated. It was found that DTNT-3DPP and DTNT(DPP-PN) 3 exhibit better light-harvesting and charge-transport properties than triphenylamine (TPA)-based star-shaped molecules. DTNT-3DPP and DTNT(DPP-PN) 3 were used as donor materials and methyl [6,6]-phenyl-C 71 -butyrate (PC 71 BM) as the acceptor for the fabrication of solution-processed bulk heterojunction (BHJ) solar cells. The power conversion efficiencies (PCEs) of solar cells based on DTNT-3DPP and DTNT(DPP-PN) 3 are 3.47 and 3.69 %, respectively. These PCEs are higher than those of the corresponding TPA-based solar cells (1.91 and 3.67 %). Our work indicates that the planar DTNT unit can improve the photovoltaic properties of organic small molecules relative to those of the TPA unit. Graphical Two novel T-shaped π-conjugated small molecules (SMs) based on 4,9-di(thiophen-2-yl)naphtho[2,3- b ]thiophene as the donor core and diketopyrrolopyrrole as acceptor arms have been synthesized. The SMs exhibit better light-harvesting and charge-transport properties than triphenylamine-based molecules. The power conversion efficiencies of solar cells based on the two SMs are 3.47 and 3.69 %.

Integrated microneedle patch and machine learning in time-resolved fluorescent immunochromatography test strip for accurate and rapid detection of Staphylococcus aureus enterotoxin B in salmon

Hui Jiang,Hui Du,Donglei Jiang,Xinmei Liu,Haijing Wu,Mengjing Huang,Rui Sun,Xiaojie Sun,Siqi Zeng,Yanmin Ju,Jun Yang

Staphylococcus aureus enterotoxin B (SEB) is a leading cause of foodborne illness. In this study, we developed a time-resolved fluorescent immunochromatography test strip (TRFITS) combined with a hydrogel microneedle patch (HMNP) and machine learning (ML) for the rapid detection of SEB in salmon. The developed HMNP, fabricated from methacrylated gelatin, can automatically extract 37 mg of interstitial fluid within 6 min. We then conducted TRFITS analysis on salmon samples to detect SEB and ML algorithms so as to predict its concentration. This method enabled efficient sampling and accurate quantification of SEB within 32 min, with a limit of detection (LOD) of 4.63 ng/mL. In addition, ML-assisted TRFITS applied to salmon demonstrated that the YOLOv11 model achieved a prediction accuracy of 91.3% and a lower LOD of 2 ng/mL. These findings highlight a novel approach toward on-site SEB detection with significant implications for food safety monitoring and point-of-care diagnostic applications.

Construction of high-capacity and high-stability La-doped VO2(B) supported on mesoporous hollow carbon spheres and their cathode performance in zinc-ion batteries

Han Shen,Xiaorong Meng,An Zhou,Zhifan Zhang,Pan Feng,Haigang Qin

This study employs a synergistic strategy combining La 3+ -doped modified VO 2 (B) (L x VO) with mesoporous hollow carbon spheres (MHCS) to prepare a high-capacity, highly stable MHCS-supported La-doped VO 2 (B) cathode material (HLVO). Optimizes the La 3+ doping ratio of L x VO and the MHCS content in HLVO, and the lattice expansion behavior of VO 2 (B) caused by La 3+ doping and the composite of carbon materials is clarified, which effectively contributes to the improvement of Zn 2+ diffusion kinetics rate and electrochemical performance of HLVO. The dynamic mechanism of LVO and HLVO is explained by density functional theory. The results show that the (110) interplanar spacing of 5 % La-doped L 0.05 VO increases to 0.355 nm. In the optimized H2L 0.05 VO, L 0.05 VO loose nanorods and MHCS are evenly distributed. The diffusion kinetic rate constant of Zn 2+ in HLVO is two orders of magnitude higher than that of VO 2 (B). At 1 A g −1 , the specific capacity of H2L 0.05 VO cathode remains at 403.5 mAh g −1 after 200 cycles, and the capacity retention rate is as high as 81.6 % after 10 A g −1 10000 cycles. The synergistic strategy of rare earth doping and carbon material composite provides a new idea for the development of high-performance aqueous zinc ion batteries (AZIBs) cathode materials.

Waste-to-wealth conversion of shrimp shells into B/N/O co-doped hierarchical porous carbon: Experimental realization and theoretical elucidation of energy storage mechanisms

Lu Luo,Shuxin Zou,Gangqi Lin,Jing Zhang,Diwei Shi

B/N/O co-doped hierarchical porous carbons were derived from waste shrimp shells. • SHPC-800 delivers an impressive specific capacitance of 426.8 F/g at 0.5 A/g. • SC device achieves a remarkable energy density of 19.58 Wh/kg at 750 W/kg. • DFT reveals differential contributions of functional groups to K adsorption capacity. The rational design of carbon derived from biomass for supercapacitors requires a deep understanding of the synergistic interplay between heteroatom doping and pore architecture. Herein, an integrated “pretreatment-co-doping-synchronous activation” strategy was proposed to fabricate B/N/O co-doped hierarchical porous carbon (SHPC) from waste shrimp shells. Leveraging the natural CaCO 3 as a template and exploiting etching effect of KOH and ammonium pentaborate tetrahydrate, the optimized SHPC-800 electrode exhibits a specific surface area of 612 m 2 /g, featuring a well-developed micro-/mesoporous network. The B/N/O co-doping upgrades the capability of the electrode by introducing additional pseudocapacitance and improving electronic conductivity, resulting in an exceptional specific capacitance of 426.8 F/g at 0.5 A/g. Moreover, a symmetric supercapacitor constructed with SHPC-800 demonstrates a remarkable energy density of 19.58 Wh/kg at 750 W/kg, along with extraordinary cycling stability. Additionally, density functional theory (DFT) results further reveal that the contributions of different functional groups to K atom adsorption and electronic regulation vary significantly, and that the performance depends not merely on the type or total amount of dopants but critically on the bonding configuration and coordination environment. These findings provide valuable insights for the structural regulation and performance optimization of porous carbons. This work not only advances the theoretical framework of heteroatom incorporation and pore architecture synergy in biomass-derived carbons but also provides a viable pathway for valorizing seafood waste into high-value electrode materials for next-generation energy storage systems. Download: Download high-res image (446KB) Download: Download full-size image

Rhodamine B/gold nanoparticles-coloaded UiO-66 as a novel probe for highly sensitive and dual-mode detection of Potato virus S in field samples

Xiangting Li,Meng Ren,Shuhang Deng,Lingli Lei,Ping Ma,Yingshuai Liu

IntroductionPotato virus S (PVS) causes severe damage to Solanaceae plants, especially potato, due to its widespread infection and the reliance on laboratory-based reverse transcription-polymerase chain reaction (RT-PCR) testing. This work intends to develop a rapid, highly sensitive, and dual-signal platform for on-site PVS detection.MethodsWe developed a highly sensitive lateral-flow immunoassay (LFIA) capable of colorimetric and fluorescent dual-mode detection based on a novel core-shell structured probe, which consists of a porous metal-organic framework UiO-66 encapsulating rhodamine B inside and loading gold nanoparticles (AuNPs) outside with 3-aminopropyltriethoxysilane (APTES) as a spacer and glue, denoted as UR@APTES@AuNPs.ResultsThe developed LFIA enabled dual-mode PVS detection within 15 min with negligible cross-interference from Potato virus X and Potato virus Y. The platform achieved a limit of detection (LOD) of 31.2 pg/mL for the colorimetric mode and 7.6 pg/mL for the fluorescent mode, over one order of magnitude lower than the conventional AuNPs-based LFIA (LOD = 462.4 pg/mL). Furthermore, PVS in field-collected potato leaves was successfully detected by the new LFIA platform.DiscussionThe dual-mode LFIA demonstrated a rapid, cost-effective, and highly sensitive on-site screening of PVS in field samples, highlighting its great potential in practical application. This platform can be easily adapted to the detection of other pathogenic viruses by using the corresponding specific antibodies.

Network-guided identification and in vitro/in vivo validation of withaferin A as an inhibitor of nuclear factor kappa B for spinal cord injury repair

Chenghu Xie,Cunxin Zhang,Ying Zhang,Liyi Chen,Xiao He,Xinzhou Xie,Kang Li,Xiaodong Wang,Chaoliang Lv

Virtual screening and experimental validation identified Withaferin A as a candidate therapy for spinal cord injury. • Withaferin A attenuates NF‑κB signaling by reducing p65 phosphorylation and promoting an anti‑inflammatory macrophage phenotype. • Molecular modeling and experimental validation confirm covalent binding of Withaferin A to p65 Cys160 via Michael addition. • Withaferin A enhances neuronal regeneration, restores motor function, preserves axonal integrity, and promotes angiogenesis in SCI mice. Background Spinal cord regeneration after injury remains a major clinical challenge due to the persistent inflammatory microenvironment associated with immune cell infiltration. Withaferin A (WA), a natural anti-inflammatory steroidal lactone with potential NF-κB-modulating activity, was identified by network-based screening as a candidate for spinal cord injury (SCI) and selected for further study. Purpose This study aimed to identify and evaluate potential anti-inflammatory small-molecule therapeutics for SCI using a network-based drug screening strategy. Study Design Experimental study combining computational drug screening, in vitro macrophage assays and in vivo SCI mouse models. Methods Network pharmacology identified 296 candidate drugs targeting 113 SCI-related genes. Five top candidates, selected for chemical diversity and accessibility, were tested in LPS-stimulated in vitro models for effects on macrophage polarization and cytokine release. Molecular docking was used to predict drug–target interactions. The lead compound, WA, was then evaluated in SCI mice for inflammation, angiogenesis, neuroregeneration and motor recovery. Results WA showed the strongest anti-inflammatory activity, dose-dependently inhibiting LPS-induced M1 polarization and TNF-α/IL-6 secretion while promoting M2 polarization and IL-4/IL-10 secretion. Integrated computational and experimental analyses identified Cys160 in a hydrophobic pocket of NF-κB p65 as the covalent binding site of WA. In vivo , WA modulated macrophage polarization, reduced inflammatory mediator secretion, increased VEGF immunoreactivity and promoted neuroregeneration and motor recovery. Conclusion WA exerts significant anti-inflammatory and neuroregenerative effects in SCI models. It also increases VEGF immunoreactivity, suggesting a potential pro-angiogenic effect after injury. These findings support WA as a promising therapeutic candidate for SCI. Download: Download high-res image (272KB) Download: Download full-size image