FOOD CHEMISTRY
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2024
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IF 8.5
Fengli Lian,Jun-Hu Cheng,Ji Ma,Da-Wen Sun
Fat reduction due to heating or cooking is an important issue in a healthy diet. In the current study, pork subcutaneous back fat was treated via microwave heating (MH) within 10—90 s and roasting - steam heating (RSH) within 2—30 min and their dynamic changes of individual adipocytes were explored by using vesicles as a bio-membrane model. The result showed that MH and RSH significantly increased fat loss ( P < 0.05), with the maximum losses being 74.1 % and 65.6 %, respectively. The mechanical strength of connective tissue decreased and then increased slightly. The microstructure demonstrated that MH and RSH treatments facilitated a large outflow of fat, showing that the particle size of the vesicle and individual adipocytes increased and then decreased. It is thus feasible to study the dynamic changes of individual adipocytes in regulating fat reduction using cell membrane simulation.
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JOURNAL OF CHROMATOGRAPHY B-ANALYTICAL TECHNOLOGIES IN THE BIOMEDICAL AND LIFE SCIENCES
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2025
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IF 2.8
Yingxia Guo,Meichen Liu,Jiye Tian,Chunpeng Feng,Qingbin Wang,Xuan Zhao,Lei Yin
As an important chemical reagent, methoxy polyethylene glycol amine (mPEG-NH 2 ) is widely used in biomedical field. Unraveling the pharmacokinetic behavior of mPEG-NH 2 polymers is essential for revealing the toxicity and efficiency of mPEG-NH 2 related drug delivery systems. In this study, a simple analytical assay based on mass spectrometry (MS) was first established and validated for quantification of mPEG 5 -NH 2 in biological matrix. The multiple reaction monitoring (MRM) transitions at m / z 252.2 (precursor ions) → 87.7 (fragment ions) and m / z 371.2 (precursor ions) → 89.2 (fragment ions) were chosen to determine mPEG 5 -NH 2 and OH-PEG 8 -OH, respectively. The UHPLC-MS/MS assay showed excellent linearity over the range of 0.01–10 μg/mL. Intra-day and inter-day accuracies and precisions of the assay were all within ± 6.44 %. The analytical assay was successfully applied to reveal the in vivo pharmacokinetic behavior of mPEG 5 -NH 2 in rats.
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SPECTROCHIMICA ACTA PART A-MOLECULAR AND BIOMOLECULAR SPECTROSCOPY
·
2024
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IF 4.3
Ruo-Fei Ma,Qi Zhang,Yue Wang,Zhang-Run Xu
An ultrasensitive strategy for in-situ visual monitoring of ATP in a single living tumor cell during mitochondria-targeted photodynamic therapy (PDT) process with high spatiotemporal resolution was proposed using surface-enhanced Raman scattering (SERS) 3D imaging technique. The nanostructures consisting of Au-Ag 2 S Janus nanoparticles functionalized with both Au nanoparticles linked by a DNA chain and a mitochondrial-targeting peptide (JMDA NPs) were deliberately employed to target mitochondria. The JMDA NPs exhibit excellent SERS activity and remarkable antitumor activity. The quantization of ATP relies on the intensity of the SERS probes bonded to the DNA, which shows a strong correlation with the generated hot spot between the Janus and the Au. Consequently, spatiotemporally controlled monitoring of ATP in the mitochondria of single living cells during the PDT process was achieved. Additionally, the JMDA NPs demonstrated remarkable capability for mitochondria-targeted PDT, providing significant antitumor effects and superior therapeutic safety both in vitro and in vivo . Our work presents an effective JMDA NPs-based SERS imaging strategy for in-situ and real-time 3D visualization of intracellular ATP in living tumor cells during the mitochondria-targeted PDT process, which enables significant information on the time point of PDT treatment and is beneficial to precious PDT applications in tumor therapy.
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SCIENCE OF THE TOTAL ENVIRONMENT
·
2025
·
IF 8
Shuteng Diao,Wenqing Ma,Yiping Wang,Xuhui Zhao,Fazhi Zhang,Xiaodong Lei
Improving saline-alkali soil requires cost-efficient and stable technologies. In this study, a novel technology combining in-situ super-stable mineralization with Ca-bentonite was successfully applied to ameliorate saline-alkali soil. A simulation experiment was conducted on Ca-bentonite in solution to validate its feasibility, and in-situ mineralization using humic acid, Fe(NO 3 ) 3 ·9H 2 O, and Ca-bentonite was performed to treat saline-alkali soil. Additionally, climate modeling and field experiments were employed to investigate the effects of this technology on soil physicochemical properties, crop growth, and crop yield. Under natural conditions, Ca-bentonite can transform into Na-bentonite via cation exchange with Na + . The results from the in-situ mineralization experiment showed that the soil pH, total content of CO 3 2− and HCO 3 − , Na + content, electrical conductivity, and bulk density decreased from approximately 10.30 to below 9.00, 7.81 to 1.53 g/kg, 7.20 to 1.51 g/kg, 2741 to 552 μS/cm, and 1.63 to 1.24 g/cm 3 , respectively. Furthermore, the germination rate of corn increased from 0 % to 83.3 % in climate simulation experiments. Field trials conducted in Inner Mongolia and Jilin, China, further demonstrated significant improvements in soil properties. The seedling emergence rates for corn and oats significantly increased, rising from 0 % to over 85 % and 95 %, respectively. Correspondingly, crop yields reached 323 kg/hm 2 for corn and 182 kg/hm 2 for oats. Together, our study introduces a novel, cost-effective, and efficient technology to enhance crop growth by mitigating soil salinity and alkalinity. This approach provides a new perspective for alleviating salt-alkali stress and contributes to the advancement of healthy and sustainable agricultural practices.
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ENVIRONMENTAL SCIENCE & TECHNOLOGY
·
2025
·
IF 11.3
Yang Li,Bo He,Jinbin Lin,Baoxue Zhou,Francesco Parrino,Mingce Long
Polymerization-based abatement of phenolic pollutants triggered by thermally activated sodium peroxydisulfate (PDS) is an effective remediation approach characterized by low oxidant consumption but restricted by narrow reaction conditions and risk of overoxidation of the organics. In particular, the polymerization is ineffective for recalcitrant phenolics and at low temperature, low initial concentration of phenolics, and high PDS dosage. The present contribution demonstrates that adding ferric chloride significantly enhances the removal efficiency, polymerization ratio, and PDS utilization efficiency, especially under the unfavorable conditions mentioned above. In the FeCl3-enhanced heat/PDS system, polymerization is promoted by iron-phenolic complexes, which favor the generation of more stable radical monomers through ligand to metal charge transfer. The ferrous ions thereby formed can accelerate PDS activation, thus synergistically contributing to polymerization. In comparison to the conventional mineralization-based advanced oxidation technologies, this process exhibits significantly higher oxidant utilization efficiency under a broad range of conditions, reducing PDS consumption by 85% when used for the treatment of real coking wastewater. Results highlight the great potentiality of this technology for the removal of phenolic pollutants and bring fundamental insights for low-carbon emission and sustainable remediation of industrial wastewater.
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FOOD CHEMISTRY
·
2025
·
IF 9.8
Yangzheng He,Xianbiao Liu,Jing Li,Liufeng Zheng,Yong Sun,Zeyuan Deng
Our previous research revealed that human milk fat substitute (HMFS) affects hepatic lipid metabolism in mice, but knowledge concerning its in vivo digestion and metabolism is limited. An in vitro infant digestion simulation and an in vivo metabolism model using Sprague Dawley rats were conducted in this study. In the in vitro model, HMFS showed better digestibility than blended vegetable oil (BVO) or blended vegetable oil plus 1,3-dioleoyl-2-palmitoyl-glycerol (BVO + OPO). In in vivo metabolism experiments, the serum concentrations of unsaturated fatty acids (C18:1 and C18:2) were markedly higher in HMFS than in BVO and BVO + OPO groups. In vivo imaging analysis confirmed that emptied from the gastrointestinal tract significantly faster than BVO and BVO + OPO. These findings suggest that incorporating HMFS in infant formula may enhance nutrient absorption and support infant development.
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APPLIED SURFACE SCIENCE
·
2025
·
IF 6.9
Wang Lin,Xianyi Lv,Qiliang Wang,Zhangcheng Liu,Liuan Li,Guangtian Zou
This study proposes a novel strategy to in-situ suppress the nitrogen incorporation during microwave plasma chemical vapor deposition (MPCVD) growth of single-crystal diamond (SCD) through nickel acetate solution bubbling. By systematically optimizing the growth parameters, SCD with nitrogen (<5 ppb) and silicon impurity concentrations below the detection limit of photoluminescence spectrum is achieved. We propose that nitrogen radicals react with nickel species in the plasma to form nickel nitrides, which are subsequently evacuated from the CVD chamber. It demonstrates that a lower dark current and a higher photocurrent are obtained for the metal–semiconductor–metal UV photodetector after nitrogen suppression. The simulation results confirm that the nitrogen-related shallow defect level ( E c − 0.6 eV) predominantly contributes to the dark current, while deep defect level ( E c − 2.5 eV) determine the photocurrent. This nickel-mediated nitrogen suppression strategy establishes a pathway for developing high-performance diamond UV photodetectors.
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ANALYTICAL CHEMISTRY
·
2025
·
IF 6.7
Lu Zhang,Yuqi Li,Jing Hu,Hanjiao Chen,Xiandeng Hou
Photochemical vapor generation (PCVG) is an effective alternative to traditional chemical vapor generation (CVG), offering superior sample introduction and matrix separation. The mechanism of PCVG involves radical reactions initiated by photolysis of the medium, which play a crucial role in the reduction and vaporization of analytes in solution. While electron paramagnetic resonance (EPR) is widely used for free radical analysis, traditional EPR methods lack in situ and real-time monitoring and may introduce instability. In this work, a comprehensive online/in situ EPR detection strategy was developed to monitor the dynamic change of free radicals during PCVG in a formic acid system. Both methods monitored the concentrations of •CO2–, •OH, •H, and •CHO radicals under light exposure. The online EPR method allows for quantification of free radicals in formic acid systems and those with common transition-metal ions. Additionally, in situ EPR tracking revealed the generation and transformation of free radicals. Decay analysis of radical adducts confirmed that the observed EPR signals result from competing processes of radical formation, interconversion, and DMPO-trap addition, indicating a dynamic equilibrium among these pathways. For the first time, the interconversion of •OH and •H radicals into •CO2– during the PCVG process was observed, shedding light on the reduction mechanism of analytes. Additionally, this finding also suggests that the reductive role of a substantial amount of •CHO in the formic-acid-based PCVG system may have been previously underestimated. Overall, this work introduces an innovative method for real-time monitoring and could provide new insight for mechanistic studies of PCVG systems.
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SEPARATION AND PURIFICATION TECHNOLOGY
·
2025
·
IF 9
Zhenxuan Zhang,Lianxiang Qiu,Yujian Li,Qingyao Zhu,Mengting Yang,Akihisa Inoue,Xiong Liang,Jiang Ma
While drinking water disinfection remains a cornerstone of public health by mitigating waterborne pathogens, the generation of carcinogenic disinfection by-products (DBPs) presents an escalating global challenge. This study systematically evaluates persulfate-driven advanced oxidation processes (AOPs) coupled with Fe-Si-B amorphous alloy ribbons as catalytic mediators in pre/post-chlorination treatment trains. Surface reconstruction of the metallic glass ribbons through persulfate exposure induced a hydrophilic transition (water contact angle: from 88.7° to 28.6°/43.9°), enhancing interfacial reactivity. Both peroxydisulfate (PDS, S 2 O 8 2– ) and peroxymonosulfate (PMS, HSO 5 – ) demonstrated effective activation by Fe-Si-B, achieving 61.2–88.7 % total organic carbon (TOC) removal via synergistic radical (SO 4 − / OH/ O 2 •− ) and non-radical (Fe(IV), 1 O 2 ) pathways. The best treatment group achieved with PDS/Fe-Si-B treatment followed by post-chlorination process which successfully reduced the formation of chlorinated-DBPs (Cl-DBPs) and brominated-DBPs (Br-DBPs) by 65.3 % and 67.4 %, respectively. Notably, asymmetric PMS preferentially oxidized bromide (Br – ) to hypobromous acid (HOBr), elevating Br-DBPs yields by 43.4 % during subsequent chlorination, indicating that PDS outperformed PMS in controlling the formation of DBPs. By contrast, the PDS/Fe-Si-B system suppressed more DBP formation by 44.1–49.5 % compared to conventional granular activated carbon (GAC) adsorption treatment, attributed to its superior pre-oxidation of DBP precursors. These findings establish metallic glass-activated persulfate systems as a dual-functional solution for simultaneous humic acid abatement and DBP mitigation in advanced water treatment.
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JOURNAL OF THE SCIENCE OF FOOD AND AGRICULTURE
·
2025
·
IF 3.5
Lei Wang,Dankui Liao,Qian Zhou,Lixia Sun,Xueping Zhang,Xiongdiao Lan,Zhangfa Tong,Meiqun Suo,Guangzhi Zhou
BACKGROUND Food-derived angiotensin-converting enzyme inhibitory peptides (ACEIPs) are commonly utilized in functional food development. In-depth research on their interactions with serum albumin can enhance their bioavailability and in vivo functionality from functional food. RESULTS This study systematically investigated the impact of the ACEIP Gly-Arg-Pro (GRP) derived from sardine muscle protein on the conformation and interaction mechanisms of bovine serum albumin (BSA) under different physiological buffer environments using multispectral techniques, molecular docking theory and isothermal titration calorimetry. GRP demonstrated potent angiotensin-converting enzyme inhibitory activity and had good biosafety in cytotoxicity assays. Steady-state and time-resolved fluorescence spectroscopy indicated the formation of a moderately bound BSA–GRP complex in various buffer environments. The existence of the BSA–GRP complex was further confirmed by ultraviolet–visible spectroscopy. Three-dimensional fluorescence spectroscopy, synchronous fluorescence spectroscopy and circular dichroism spectroscopy demonstrated that adding GRP to BSA affected its microenvironment and structural conformation, with more significant changes observed in the HEPES buffer system due to its distinct physicochemical properties. The thermodynamic parameters indicated that the interaction was mainly driven by hydrophobic forces, making it a spontaneous process regarding Gibbs free energy change. Molecular docking theory showed that GRP is preferentially bound to site II of subdomain IIIA. CONCLUSION This work established a research system for BSA–GRP interactions and obtained relevant parameters, which will benefit the subsequent development of nutritional supplements and functional foods. © 2025 Society of Chemical Industry.
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Agronomy-Basel
·
2025
·Vol. 15
·期 8
·1789
·
IF 3.4
Kaiyan Zhang,Haitao Li,Hongtao Hang,Xinhua He,Yanyou Wu
Ammonium (NH4+) toxicity impairs plant growth, but nitrate (NO3−) can mitigate this effect through unresolved mechanisms. Using leaf δ13C values (photosynthetic capacity) and a bidirectional15N tracer (NH4+assimilation efficiency and source utilization), this study investigated these mechanisms in 35-day-oldOrychophragmus violaceusplantlets grown in modified Murashige and Skoog media under varying NH4+:NO3−ratios.15N isotope fractionation during NH4+(same fixed 20 mM NH4Cl) assimilation decreased with increasing NO3−supply (10, 20, and 40 mM NaNO3). Under 20 mM NH4+(δ15N = −2.64‰) at two15NO3−-labels (δ15N-NO3−= 8.08‰, low15N,L) and (δ15N-NO3−= 22.67‰, high15N,H), increasing NO3−concentrations enhanced NO3−assimilation, alleviating acidic stress from NH4+and improving photosynthesis. Higher NO3−levels also increased NH4+utilization efficiency, reducing futile NH4+cycling and decreasing associated15N fractionation during assimilation. Our results demonstrate that NO3−alleviates NH4+toxicity primarily by enhancing photosynthetic performance and optimizing NH4+utilization efficiency.
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PESTICIDE BIOCHEMISTRY AND PHYSIOLOGY
·
2025
·
IF 4
Benshui Shu,Yuting Huang,Xinyi Xie,Cuiting Liu,Jintian Lin,Ying Xiao,Jingjing Zhang
Azadirachtin, a highly effective botanical pesticide, demonstrated notable biological activities against Spodoptera frugiperda , including mortality induction, growth and development inhibition, and antifeedant effects. Neuropeptide F (NPF) has been shown to play a role in various physiological processes in insects. Nonetheless, the functions of Sf- NPF1 in regulating food intake and antifeedant induction by azadirachtin in S. frugiperda larvae are yet to be elucidated. In this study, the antifeedant effects induced by 0.5 and 1.0 μg/g azadirachtin treatments in S. frugiperda larvae were evaluated. The sequence of Sf-NPF1 was identified with two transcript variants, and the proteins exhibit high identity with NPFs found in lepidopteran insects. Spatiotemporal expression analysis indicated that Sf-NPF1 exhibited high expression levels in first instar larvae and male adults, while the lowest expression was observed in non-feeding stages (egg and pupa stages). Besides, Sf-NPF1 also demonstrated notable expression in the midgut tissue of larvae. Azadirachtin treatments inhibited the expression of Sf-NPF1 in the larval midgut. The injection of the Sf- NPF1a mature peptide resulted in increased larval food intake and weight gain during a two-day treatment period. The antifeedant effect induced by 1.0 μg/g azadirachtin was also mitigated by the injection of Sf- NPF1a mature peptide. Our results indicated that Sf- NPF1 may serve as a positive regulator of food intake in S. frugiperda larvae, while azadirachtin could exert an antifeedant effect in larvae via inhibiting the expression of Sf-NPF1 . These findings establish a foundation for comprehending the mechanism behind the antifeedant effect induced by azadirachtin in insect larvae and provide a potential target for developing novel insecticides.
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