JOURNAL OF CHEMICAL AND ENGINEERING DATA
·
2020
·
IF 2.369
Shengzheng Guo,Yang He,Gang Wang,Weibing Dong,Xuxing Wan,Yanxiao Zhao,Shichao Du
The solubility of N-acetyl-l-leucine in 12 mono organic solvents (methanol, ethanol, n-propanol, 2-propanol, 1-butanol, isobutanol, 2-butanol, methyl acetate, ethyl acetate, propyl acetate, acetone, and acetonitrile) was determined by the gravimetric method over the temperatures ranging from 283.15 to 323.15 K at 0.1 MPa. The solubility of N-acetyl-l-leucine in each experimental solvent increases with increasing temperature. Moreover, the solubility is an order of magnitude higher in alcohol solvents than in other solvents, which is mainly attributed to the hydrogen bond formation. In addition, the experimental solubility data are correlated by three models, including the modified Apelblat equation, λh equation, and the nonrandom two-liquid (NRTL) model. All of the average relative deviation % values are less than 3.72%, which indicates that the three models have satisfactory correlations. Furthermore, the mixing thermodynamic properties of N-acetyl-l-leucine in 12 mono solvents including ΔmixG0, ΔmixH0, and ΔmixS0 are calculated based on the NRTL model, indicating that the mixing process was spontaneous.
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Journal of Food Measurement and Characterization
·
2020
·Vol. 14
·期 3
·1461-1469
·
IF 1.648
Tian Yanhua,Du Huizhi,Qing Xiaohuan,Zhang Liwei
The leaves of Diospyros lotus L. have many bioactivities such as antioxidant, liver protection and anti-inflammation, in which flavonoids are the main active components. In the study, high performance liquid chromatography (HPLC) method was established to simultaneously determine the contents of eight flavonoids, including myricetin, quercetin and their monoglycoside in the Diospyros lotus L. leaves. The methodological verification showed that the developed method had good linearity, precision, accuracy and reproducibility. During the leaves of Diospyros lotus L. picked from April to October, the contents of eight flavonoids in the samples picked in May were significantly higher than those in other times, and myricitrin content was highest. The total amount of eight flavonoids in freeze-dried samples was significantly higher than those of samples processed by shadow-dried at room temperature and oven-dried at 40 °C. Antioxidant activity of the sample was significantly affected by the picking time and drying method. The antioxidant activities were linearly correlated with the contents of the total flavonoids and myricitrin (Mytr), indicating that Mytr was the main antioxidant ingredient of sample. In a word, the paper lays a foundation for the qualitative and quantitative analysis of eight flavonoids in the leaves of Diospyros lotus L., the preparation of active ingredients and the study on the quality standards of the leaves.
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ACS Biomaterials Science & Engineering
·
2020
·
IF 4.152
Qi Wang,Zhen Shi,Yufeng Shou,Kunxi Zhang,Guifei Li,Pengfei Xia,Shifeng Yan,Jingbo Yin
Supramolecular hydrogels formed by noncovalent bonds are attractive “smart” materials, which can rapidly respond to external stimuli. However, only a handful of supramolecular hydrogels is applicable in tissue engineering, due to the instability and poor mechanical strength of noncovalent cross-linking hydrogels. Thus, a rigid and stable supramolecular hydrogel has been developed based on poly(l-glutamic acid) and 2-ureido-4[1H]pyrimidinones (UPy), and the UPy stacks are noncovalent cross-linking interactions. The hydrogels show excellent mechanical strength and stability, in sharp contrast to noncovalent hydrogels cross-linked by UPy dimers and covalent hydrogels cross-linked by esterification. The hydrogels also exhibit remoldability, self-healing, and thermoplastic printing characteristics, which are caused by the reversible supramolecular property of UPy stacks. Also, the formation of hydrogels dependent on UPy stacks is further investigated by atomic force microscope, small-angle X-ray scattering, in situ X-ray diffraction, circular dichroism, and UV–vis spectroscopies. Finally, the hydrogels show commendable biocompatibility and degradability, which have high potential applications in regenerative medicine.
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高影响因子
AQUACULTURE RESEARCH
·
2020
·Vol. 51
·期 3
·1012-1025
·
IF 1.748
Xiao Yan,Chaobin Qin,Dapeng Deng,Guokun Yang,Junchang Feng,Ronghua Lu,Liping Yang,Xiaolin Meng,Xianglin Cao,Guoxing Nie,Guiqin Wang
Apelin and its receptor apj have a variety of biological functions and are involved in appetite and energy regulation. However, the comparative studies of apelin/apj system in fish are still absent. In this study, the open reading frames of apelin and apja and apjb were obtained. Apelin and apj mRNAs were widely distributed in all tissues detected, with the highest level in the brain, followed by the spleen, kidney and foregut. The mRNA expression of apelin and apj s increased rapidly at mealtime and significantly decreased after 30 min of feeding. In addition, there were obvious circadian rhythms (higher at nighttime and lower at daytime) in the expression of apelin and apja and apjb . Furthermore, fasting elevated the mRNA levels of apelin and apj s in the hypothalamus, foregut and hepatopancreas. When the 8-day fasted fish were refed, apelin and apj s mRNA expression significantly decreased. However, there were differences in the response to fasting and refeeding between apja and apjb . The roles of apelin and its receptor apjs in fish are poorly understood. Herein, we employed molecular and physiological studies to characterize their structures, distribution and mRNA expression responses to periprandial changes and fasting–refeeding in Cyprinus carpio L. for the first time.
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COLLOIDS AND SURFACES B-BIOINTERFACES
·
2020
·
IF 4.389
Anlin Yin,Weihua Zhuang,Gongyan Liu,Xiaorong Lan,Zhonglan Tang,Yan Deng,Yunbing Wang
The fabrication of a functional small-diameter vascular graft with good biocompatibility, in particular hemocompatibility, has become an urgent clinical necessity. We fabricated a native bilayer, small-diameter vascular graft using PEGylated chitosan (PEG-CS) and poly (L-lactic acid-co-ε-caprolactone; PLCL). To stabilize the inner layer, a PEG-CS blend with PLCL at ratio of 1:6 was casted on a round metal bar by a drip feed, and the outer layer, a PLCL blend with water-soluble PEG that acted as a sacrificial part to enhance pore size, was fabricated by electrospinning. The results showed excellent hemocompatibility and strong mechanical properties. In vitro , the degradation of the graft was evaluated by measuring the graft structure, mass loss rate, and changes in molecular weight. The results indicated that the graft had adequate support for the regeneration of blood vessels before collapse. An in vivo study was performed in a canine femoral artery model for up to 24 weeks, which demonstrated that the PEGylated bilayer grafts possessed excellent structural integrity, high compatibility with blood, good endothelial cell (EC) and smooth muscle cell (SMC) growth, and high expression levels of angiogenesis-related proteins, features that are highly similar to autologous blood vessels. Moreover, the results showed almost negligible calcification within 24 weeks. These findings confirm that the bilayer graft mimics native cells, thereby effectively improving vascular remodeling.
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FOOD CHEMISTRY
·
2021
·
IF 7.514
Limin Wang,Yunsen Zhang,I. Agbaka Johnpaul,Kai Hong,Yumei Song,Xiao Yang,Chenyan Lv,Changwei Ma
Hops are abundant in natural bioactive compounds. In this work, nine prenylated bitter compounds from hop were evaluated for their inhibitory activity against α-glucosidase. As a result, four flavonoids and one phloroglucinol (lupulone, LP) outperformed acarbose in inhibiting α-glucosidase. Isoxanthohumol (IX) and LP with two types of structures were selected for inhibition mechanism studies by spectroscopic methods and molecular dynamics simulation (MD). Results showed that IX acted as noncompetitive inhibitor and bound to α-glucosidase in allosteric sites via hydrogen bonds, hydrophobic, van der Waals (vdW), and electrostatic force, whereas LP was uncompetitive inhibitor and bound to catalytic sites via hydrophobic and vdW interactions. Notably, the conformation around binding site of α-glucosidase formed stable α-helix and tightened after binding IX and LP, respectively, which helped to elucidate noncompetitive and uncompetitive inhibitory mechanisms. This work demonstrated that two types of prenylated bitter compounds are discrepant in their mechanisms of interaction with α-glucosidase.
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高影响因子
INTERNATIONAL JOURNAL OF FOOD SCIENCE AND TECHNOLOGY
·
2021
·Vol. 57
·期 1
·92-100
·
IF 3.713
Wei Wang,Lijun Fan,Xuewen Li,Zirong Wang,Jianzhong Zhou,Buligen Jialengbieke,Jianye Ren,Xiaodong Hu
In this study, the digestion of pectic polysaccharide from Brassica rapa L. (BRP) in vitro and its effect on the intestinal microbiota in cyclophosphamide (Cy)-treated mice were investigated. As a result, the molecular weight ( M w ) of BRP was significantly decreased after simulated gastrointestinal digestion, and content of reducing sugars ( C R ) was significantly increased ( P < 0.05), implying that BRP was gradually degraded under gastrointestinal digestion conditions in vitro . In vivo , both 50 or 200 mg kg −1 ·BW/d of BRP significantly improved SCFAs concentrations that decreased by Cy treatment ( P < 0.05). Meanwhile, BRP enriched the alpha-diversity of intestinal microbiota and altered the microbial composition with an increase of Lactobacillus and a decrease of Clostridium, Ruminococcus, Oscillospira ( P < 0.05). Besides, BRP intervention was conducive to the recovery of mouse body weight, thymus and spleen indices, and contributed to improve the intestinal barrier.
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ACS Sensors
·
2021
·
IF 7.711
Qiuni Zhao,Deming Sun,Si Wang,Zaihua Duan,Zhen Yuan,Guangfen Wei,Jian-Long Xu,Huiling Tai,Yadong Jiang
Titanium carbide (Ti3C2Tx) with a distinctive structure, abundant surface chemical groups, and good electrical conductivity has shown great potential in fabricating superior gas sensors, but several challenges, such as low response kinetics, poor reversibility, and serious baseline drift, still remain. In this work, γ-poly(l-glutamic acid) (γ-PGA) with a blocking effect is exploited to modify Ti3C2Tx, thereby stimulating the positive response behavior of Ti3C2Tx and improving its gas sensing performance. On account of the unique synergetic interaction between Ti3C2Tx and γ-PGA, the response of the flexible Ti3C2Tx/γ-PGA gas sensor to 50 ppm NO2has been improved to a large extent (average 1127.3%), which is 85 times that of Ti3C2Tx (only 13.2%). Moreover, the as-fabricated Ti3C2Tx/γ-PGA sensor not only exhibits a shorter response/recovery time (average 43.4/3 s) compared with the Ti3C2Tx-based sensor (∼18.5/18.3 min) but also shows good reversibility and repeatability (relative standard deviation (RSD) <1%) at room temperature within 50% relative humidity (RH). The improved gas sensing properties of the Ti3C2Tx/γ-PGA sensor can be attributed to the enhancement of effective adsorption and the blocking effect assisted by water molecules. Furthermore, the gas sensing response of the Ti3C2Tx/γ-PGA sensor is studied at different RHs, and humidity compensation of the sensor is carried out using the multiple regression method. This work demonstrates a novel strategy to enhance the gas sensing properties of Ti3C2Tx by γ-PGA modification and provides a new way to realize highly responsive gas detection at room temperature.
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Biofabrication
·
2021
·Vol. 13
·期 4
·045018
·
IF 10.02
Shaohua Wu,Jiao Liu,Jiangyu Cai,Jinzhong Zhao,Bin Duan,Shaojuan Chen
Fiber constructed yarns are the elementary building blocks for the generation of implantable biotextiles, and there are always needs for designing and developing new types of yarns to improve the properties of biotextile implants. In the present study, we aim to develop novel nanofiber yarns (NYs) by combining nanostructure that more closely mimic the extracellular matrix fibrils of native tissues with biodegradability, strong mechanical properties and great textile processibility. A novel electrospinning system which integrates yarn formation with hot drawing process was developed to fabricate poly(L-lactic acid) (PLLA) NYs. Compared to the PLLA NYs without hot drawing, the thermally drawn PLLA NYs presented superbly-orientated fibrous structure and notably enhanced crystallinity. Importantly, they possessed admirable mechanical performances, which matched and even exceeded the commercial PLLA microfiber yarns (MYs). The thermally drawn PLLA NYs were also demonstrated to notably promote the adhesion, alignment, proliferation, and tenogenic differentiation of human adipose derived mesenchymal stem cells (hADMSCs) compared to the PLLA NYs without hot drawing. The thermally drawn PLLA NYs were further processed into various nanofibrous tissue scaffolds with defined structures and adjustable mechanical and biological properties using textile braiding and weaving technologies, demonstrating the feasibility and versatility of thermally drawn PLLA NYs for textile-forming utilization. The hADMSCs cultured on PLLA NY-based textiles presented enhanced attachment and proliferation capacities than those cultured on PLLA MY-based textiles. This work presents a facile technique to manufacture high performance PLLA NYs, which opens up opportunities to generate advanced nanostructured biotextiles for surgical implant applications.
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CHEMICAL ENGINEERING JOURNAL
·
2021
·
IF 13.273
Li Yang,Haoshuang Wu,Yuqi Liu,Qiongfen Xia,Yuan Yang,Nuoya Chen,Ming Yang,Rifang Luo,Gongyan Liu,Yunbing Wang
Endowing cardiovascular stents with multiple functionalities, such as anti–proliferative, and anti–inflammatory properties, is important for combating restenosis after stent implantation. The surface properties of stents play a key role in obtaining the desired post–implantation performance. Zwitterionic coatings are resistant to non–specific adsorption, and here, a mussel–inspired zwitterionic poly (carboxybetaine acrylate– co –dopamine methacrylate) copolymer (PCBDA) was synthesized and applied to the surface of a biodegradable poly( l –lactide) stent. To obtain a zwitterionic polymer coating, the surface of a PLA stent was pretreated by co–depositing polydopamine (PDA) and polyethyleneimine (PEI). The mussel–inspired catechol moieties in the PCBDA copolymer cross–linked with the PDA–PEI hybrid to form a robust PCBDA/PDA–PEI coating on the PLA stent. The zwitterionic PCBDA formed a hydration layer once it contacted blood, preventing the non–specific adsorption of serum proteins, which further blocked subsequent coagulation and inflammation. The anti–protein adsorption tests showed that the PCBDA/PDA–PEI coating resisted the non–specific adsorption of fibrinogen, which was a positive signal for enhanced anti–coagulation. Moreover, the platelet adhesion/activation tests confirmed its antithrombotic properties. The in vivo tissue response was also greatly suppressed due to the inhibited recognition of PCBDA/PDA–PEI–coated implants by macrophages. Interestingly, unlike traditional zwitterionic polymers, due to the introduction of adhesive catechol moieties, PCBDA supported the growth of endothelial cells, demonstrating its ability to selectively direct the fate of endothelial cells and smooth muscle cells. In vivo stent implantation results showed inhibited intimal hyperplasia due to the protection of PCBDA/PDA–PEI. Overall, due to the specific characteristics of the mussel–inspired PCBDA/PDA–PEI coating, anticoagulation, anti–inflammatory, and anti–proliferation properties were obtained; thus, it is expected that this coating can be used to inhibit restenosis and realize endothelialization after stent implantation.
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JOURNAL OF AGRICULTURAL AND FOOD CHEMISTRY
·
2019
·
IF 3.571
Dongwen Hu,Tao Bao,Yang Lu,Hongming Su,Huihui Ke,Wei Chen
This study was aimed to investigate the protective effects of three different mulberry fruit polysaccharide fractions (MFP-I, MFP-II, and MFP-III) against palmitic acid (PA)-induced hepatocyte lipotoxicity and characterize the functional polysaccharide fraction using gel permeation chromatography, high-performance liquid chromatography, Fourier transform infrared spectroscopy, and nuclear magnetic resonance analyses. MFP-I, MFP-II, and MFP-III were isolated from mulberry fruit by stepwise precipitation with 30, 60, and 90% ethanol, respectively. MFP-II at 0.1 and 0.2 mg/mL dramatically attenuated PA-induced hepatic lipotoxicity, while MFP-I and MFP-III showed weak protection. It was demonstrated that MFP-II not only increased nuclear factor erythroid-2-related factor 2 (Nrf2) phosphorylation and its nuclear translocation, thereby activating the Nrf2/ARE signaling pathway, but also enhanced heme oxygenase 1, NAD(P)H:quinone oxidoreductase 1, and γ-glutamate cysteine ligase gene expressions and promoted catalase and glutathione peroxidase activities, which protected hepatocytes against PA-induced oxidative stress and lipotoxicity. Further investigation indicated that the molecular weight of MFP-II was 115.0 kDa, and MFP-II mainly consisted of galactose (30.5%), arabinose (26.2%), and rhamnose (23.1%). Overall, our research might provide in-depth insight into mulberry fruit polysaccharide in ameliorating lipid metabolic disorders.
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AMB Express
·
2019
·Vol. 9
·期 1
·1-11
·
IF 2.226
Guo Zongren,Long Liangkun,Ding Shaojun
d -Mannose and l -ribose are two important monosaccharides, which have attracted public attention recently because of their great application potentials in food, cosmetic and pharmaceutical industries. Sugar isomerases catalyze the sugar isomerization and therefore can be used as the biocatalysts for production of the high-value sugars from inexpensive sugars. l -arabinose isomerase catalyzes the conversion of l -arabinose to l -ribulose, while d -lyxose isomerase catalyzes l -ribulose and d -fructose to l -ribose and d -mannose, respectively. In this paper, a putative d -LI from Bacillus velezensis (BvLI) was identified, characterized and used to produce d -mannose and l -ribose from d -fructose and l -arabinose, respectively. The recombinant BvLI exhibited a maximum activity at 55 °C and pH 6.5, in the presence of 0.1 mM Co 2+ . Approximately 110.75 g/L d -mannose was obtained from 500 g/L d -fructose in 6 h by the recombinant BvLI, and approximately 105 g/L l -ribose was obtained from 500 g/L l -arabinose in 8 h by the successive biocatalysis of l -arabinose isomerase from Bacillus licheniformis (BlAI) and BvLI.
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危险品化学品经营许可证（带存储）