JOURNAL OF COLLOID AND INTERFACE SCIENCE
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2025
·
IF 9.7
Hao-yu Zhang,Jing-yuan Wang,Jian-jun Li,Jian Zhu,Guo-jun Weng,Ya-li Li,Jun-wu Zhao
Non-specificity and inadequate quantitative capability are the primary challenges faced by the surface-enhanced Raman scattering (SERS) technique, especially when it comes to detecting bacteria in real samples. Herein, a novel face-centered Au Convex Polyhedral Nanocrystal (Au CPNC) with high-index facets and its assembly Au CPNCs microarray chip were designed and fabricated to address these challenges, within the process where 4-mercaptophenylboronic acid (4-MPBA) was utilized as a multifunctional element. The as-prepared Au CPNC possesses anisotropic raised edges enjoying tunable localized surface plasmon resonance modes for SERS enhancement. Then we obtained long-region ordered Au CPNCs microarrays equipping even greater “hot spots” with a SERS enhancement factor (EF) up to 5.38 × 10 7 . The constructed SERS probes excellently Leveraged the outstanding SERS performance of Au CPNC and the superior functions of 4-MPBA, which enabled the differences among the bacterial “fingerprints” to be highlighted. Through partial least squares discriminant analysis (PLS-DA), we successfully identified Escherichia coli, Staphylococcus aureus, Pseudomonas aeruginosa and Listeria monocytogenes with achieving limits of detection (LODs) in spiked whole blood samples of 3, 1, 2, and 2 cfu/mL, respectively. Notably, the LODs for all samples by SERS mapping visual readout mode were as low as 10 cfu/mL. In practical applications, our method demonstrated its efficacy by 100 % accurately classifying (20 cases) of real blood samples. Altogether, the theoretical significance and application value of this study reside in providing fundamental insights and approaches for the development of pathogenic bacteria detection field.
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高影响因子
TALANTA
·
2024
·
IF 5.6
Yutong Wang,Qianchun Zhang,Mengping Huang,Ganggang Ai,Xiaofeng Liu,Yuqi Zhang,Ran Li,Jie Wu
Stroke ranks as the second leading cause of disability and mortality globally. Biomarker detection represents a promising avenue for predicting disease severity and prognosis. The expression levels of metalloproteinase-9 (MMP-9), neuron-specific enolase (NSE), and N-terminal pro-brain natriuretic peptide (NT-pro BNP) in blood correlate with stroke severity. Hence, monitoring these biomarkers is crucial for stroke diagnosis and management. Point-of-care testing (POCT) offers on-site diagnostic capabilities, with lateral flow immunoassay (LFIA) being the most widely used method currently. However, traditional LFIA sensitivity requires enhancement. This study introduces an ultra-sensitive surface-enhanced Raman scattering-based lateral flow immunoassay (SERS-based LFIA) strip for simultaneous detection of the three stroke biomarkers using SERS immune tags. Bimetallic core-shell structured SERS immune tags leverage the advantages of two metals, ensuring stability and enhancing Raman signals through plasmon resonance. This development of a POCT based on SERS-based LFIA strips offers rapid, sensitive, and multiplex detection of stroke biomarkers. The limits of detection (LODs) for MMP-9, NSE, and NT-pro BNP were 0.00020 ng mL −1 , 0.00016 ng mL −1 , and 0.00012 ng mL −1 , respectively. Furthermore, enzyme-linked immunosorbent assay (ELISA) validated the accuracy of SERS-based LFIA. Clinical sample analysis demonstrated consistency between outcomes obtained by SERS-based LFIA and ELISA. Thus, SERS-based LFIA presents a novel POCT approach for stroke diagnosis.
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MICROCHEMICAL JOURNAL
·
2025
·
IF 5.1
Yi He,Xiaoyu Yang,Shunbi Xie,Runzi Zhang,Yao Liu,Mengjun Wang
Roxithromycin (ROX) is an effective antibiotic, but its prolonged administration may lead to hepatotoxicity and other side effects. To address this concern, the present study introduced a novel strategy that integrated a CHA-mediated dual-end DNA walker with hydrogen-bonded organic frameworks (HOFs) combined with Au nanoparticles (NPs) as efficient SERS substrates, enabling highly sensitive detection of ROX in food. Notably, HOF possessed a large specific surface area and strong adsorption properties that making it excellent platforms for the in-situ synthesis of abundant Au NPs and contributed to robust and uniform SERS signals. When ROX was present, it can trigger the activation of CHA and resulting the formation of a dual-end DNAzyme structure. Meanwhile, under the catalytic action of Mg 2+ , DNAzyme initiated the DNA walker mechanism and generated a substantial amount of Output DNA. Moreover, the Au@4-NTP@Ag core-shell structure generated numerous SERS “hot spots” and combined with the signal-amplifying capability of HOF@Au, leading to a remarkable improvement in SERS sensitivity. Based on this mechanism, the developed sensor employs a “signal-on” strategy for ultra-sensitive detection of ROX, achieving a detection range from 1.0 × 10 −6 to 1.0 × 10 −13 mol/L with a detection limit of 3.72 × 10 −14 mol/L. Furthermore, the method had been successfully applied to the detection of ROX in real food samples, including milk and honey, offering a promising and innovative framework for the development of next-generation SERS biosensors. This approach demonstrated significant potential for highly selective and sensitive antibiotic monitoring in food safety applications.
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JOURNAL OF FOOD COMPOSITION AND ANALYSIS
·
2025
·
IF 4.6
Xiaoyu Yang,Youlin Tang,Runzi Zhang,Yao Liu,Mengjun Wang,Juan Zhang,Yi He
Chloramphenicol (CAP) is an antibiotic that has the potential to induce irreversible aplastic anemia. Therefore, developing sensitive detection methods for CAP has become crucial. In this study, an aptasensor for the detection of CAP was developed by integrating catalytic precipitation reactions using a gold (Au)-manganese (Mn)-copper (Cu) nanozyme with surface enhanced Raman spectroscopy (SERS). First, a trimetallic nanozyme composed of Au, Mn, and Cu was synthesized, exhibiting horseradish peroxidase-like activity. Subsequently, target CAP induced catalytic hairpin assembly (CHA) cycle can open a large number of hairpin H1, enabling the binding of nanozyme@H2 to the SERS substrate Fe 3 O 4 @SiO 2 @Ag@4-chloro-1-naphthol (4-CN). The trimetallic nanozyme can catalyze the conversion of 4-CN into benzo-4-chloro-hexadienone (4-CD), which further initiates catalytic precipitation reactions (NCP), resulting in a decrease in the Raman signal of 4-CN. By this way, the aptasensor could achieve a sensitivity at 3.52 × 10 -13 mol/L, offering a novel strategy for the detection of CAP in food products.
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MICROCHEMICAL JOURNAL
·
2025
·
IF 5.1
Yao Liu,Runzi Zhang,Xiaoyu Yang,Yi He,Xiao Yang
Herein, a dual-enhanced SERS biosensor has been developed for the detection of bisphenol S (BPS) based on silver nanoflowers coated zeolitic imidazolate-based metal-organic framework (AgNFs@ZIF-8) cooperate with host-guest recognition amplification technology. The biosensor utilizes the molecularly imprinted polymer (MIP) and BPS aptamer chain (APT) as dual recognition element to achieve a significant increase in selectivity. In the presence of the target, the APT on AgNFs@ZIF-8@β-cyclodextrin(β-CD) specifically binds with BPS, which further recognized by MIP to form a sandwich structure. At this time, due to the large surface area, and stable Raman enhancement ability, AgNFs@ZIF-8@β-CD could not only provide multiple active “hot spots” as the surface-enhanced Raman scattering (SERS) enhanced substrate, but also specific capture large number of methylene blue (MB, SERS tag) through the host-guest recognition. Moreover, multiple host-guest recognition can be achieved by further addition of AgNFs@ZIF-8@β-CD and MB, which greatly improve the detection sensitivity. This SERS sensor, characterized by an extensive linear detection range from 1.00 × 10 −13 to 1.00 × 10 −6 M and an exceptionally low limit of detection (LOD) of 1.36 × 10 −14 M, has been effectively utilized for the detection of BPS in plastic pacifiers, receipts, and river water samples. As an extremely sensitive and highly specific analytical method for detecting BPS, this SERS-based method has promising applications in the fields of environmental monitoring and food safety.
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MICROCHIMICA ACTA
·
2017
·Vol. 185
·期 1
·1-9
·
IF 4.58
Zhu Tingfeng,Hu Yongjun,Yang Kang,Dong Ning,Yu Meng,Jiang Ningjing
The authors report on a surface-enhanced Raman scattering (SERS) nanoprobe for the specific detection of Escherichia coli O157:H7 ( E. coli O157:H7). Gold nanoparticles (AuNPs) were coated with a thick silica shell, and the Raman reporter (4,4′-dipyridyl) was embedded between gold nanoparticle and silica shell. This technique prevents any external effects on the AuNPs from the environment and avoids any interaction between the Raman reporter and possible impurities. Compared to the SERS nanoprobe of the conventional AuNP/AgNP type, the present nanoprobe can be applied in complex biochemical circumstances without aggregation because of its excellent stability. The SERS nanoprobeis stable for up to 50 h. The probe was applied to the SERS detection of E. coli O157:H7 by exploiting the Raman band peaking at 1612 cm −1 of 4,4′-dipyridyl (DP). The limit of detection is as low as 10 CFU·mL −1 . The technique was successfully employed to the detection of E. coli O157:H7 in real samples, achieving recoveries between 95.5 and 114.8%.
A novel surface-enhanced Raman scattering (SERS) nanoprobe based on the silica encapsulated gold nanoparticles (SEGNs) combining with the functionalized magnetic nanoparticles (MNPs), detecting E. coli O157:H7 with high sensitivity and specificity.
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Chemosensors
·
2024
·Vol. 12
·期 7
·137
·
IF 3.7
Zhixiong Lao,Mingmin Zhong,Yin Liang,Jianrong Tan,Xiaoyan Liang,Wucheng Xie,Yong Liang,Jun Wang
Sulfide organics and phenols are ubiquitous in freshwater lakes all over the world. As two taste and odor (T and O) compounds, they are harmful to the environment and human body. The existing detection methods for T and O compounds mainly include sensory analysis and gas-phase mass spectrometry, which are cumbersome and time-consuming. Herein, a method for the simultaneous and rapid detection of two T and O compounds (methyl sulfide and 2,4-di-tert-butylphenol) based on surface-enhanced Raman spectroscopy (SERS) is firstly developed. The SERS substrate was prepared by coating Ag nanoparticles (Ag NPs), layered double hydroxide (LDH), and graphene oxide (GO) on the surface of an Ag-coated Au nanoparticle (Au@Ag NP) substrate. Under optimal conditions, this SERS substrate possessed low detection limits of 1.53 ppm for methyl sulfide and 0.39 ppm for 2,4-di-tert-butylphenol. In addition, it took only 20 min to complete the detection using this method, without complex sample pretreatment. Furthermore, it was successfully applied to simultaneously detect methyl sulfide and 2,4-di-tert-butylphenol in actual water samples and had good application prospects for the rapid detection of T and O compounds in water.
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CHEMICAL ENGINEERING JOURNAL
·
2026
·
IF 12.5
Shiqiang Wang,Changkun Qiu,Huiyun Jiang,Yan Jin,Haozhi Wang,Liang Zhu
An Au film–PIL nanohole–SiO 2 nanosphere–Au nanosphere SERS substrate integrated with machine learning was fabricated. • Dual electromagnetic enhancement was realized by waveguide modes of hybrid structure and LSPR of Au nanospheres. • PIL nanoholes around Au nanospheres promoted PAHs adsorption through hydrophobic effect and π-π stacking interaction. • The PCA-SVM model achieved over 95% prediction accuracy for PAHs of various concentrations and species in river water. Surface-enhanced Raman spectroscopy (SERS) is a promising trace detection technique, yet its practical application in environmental pollutant analysis remains severely restricted by insufficient electromagnetic enhancement and weak adsorption capacity toward target analytes. Herein, a novel hybrid SERS substrate with dual electromagnetic enhancement and highly efficient molecular enrichment was facilely fabricated, featuring a hierarchical structure of Au film–poly(ionic liquid) (PIL) nanohole–SiO 2 nanosphere–Au nanosphere. In this system, the waveguide optical modes of the Au film–PIL nanohole–SiO 2 nanosphere structure induce the primary electromagnetic enhancement, while Au nanospheres are precisely positioned in the primary enhancement regions to deliver a secondary electromagnetic enhancement through localized surface plasmon resonance (LSPR), thereby realizing a high enhancement factor of 2.35 × 10 7 for pyrene. Meanwhile, PIL nanoholes facilitate specific adsorption and enrichment of polycyclic aromatic hydrocarbons (PAHs) within hotspots via the hydrophobic effect and π-π stacking interactions, significantly amplifying the SERS response of analytes. The hybrid substrate exhibits excellent analytical performance, with limits of detection (LODs) as low as 5.33 × 10 −10 mol/L for pyrene and 3.97 × 10 −10 mol/L for anthracene, respectively. Furthermore, a combined principal component analysis (PCA) and support vector machine (SVM) algorithm was employed to efficiently extract spectral features and reduce data dimensionality, achieving a prediction accuracy exceeding 94% for varying concentrations and species of PAHs in actual river water samples. Taken together, the combination of this hybrid SERS substrate and the PCA-SVM algorithm provides a novel sensing platform, enabling ultrasensitive, accurate, and intelligent trace-level detection of environmental pollutants in complex matrices. Download: Download high-res image (185KB) Download: Download full-size image
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OPTICS AND LASER TECHNOLOGY
·
2024
·
IF 4.6
Meng Shi,Xiaobing Liu,Xinshuang Wang,Liang Shang,Wei Wang,Guangqiang Liu
In this manuscript, a uniform and well-organized array of sea anemone cap cavities array (SCCA) is meticulously fabricated on the fiber end face using nanosphere lithography (NSL) and reactive ion etching (RIE) techniques. The nano-cavities possess strong light capture capabilities, greatly improving light utilization through multiple reflections. The characterization, formation process and formation mechanism of SCCA were thoroughly investigated. Compared to traditional open cavities, the theoretical maximum electromagnetic field intensity at the base of the new close-packed cavity within SCCA is 6.15 times higher, while the local electric field within the cavity is 9.7 times greater, indicating superior light trapping capability and hot spot generation. The rugged sea anemone-like structure atop SCCA fosters abundant SERS “hot spots,” further enhancing local surface plasmon resonance (LSPR), resulting in exceptional SERS performance. By utilizing 4-aminothiophene (4-ATP) as an in-situ Raman probe molecule, SCCA demonstrates high-sensitivity detection capability down to a concentration of 10 −11 M with excellent reproducibility and stable performance across multiple detections. Therefore, fiber optic SERS probes offer promising potential for high-sensitivity in-situ detection methodologies in areas such as food safety.
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SENSORS AND ACTUATORS B-CHEMICAL
·
2023
·
IF 8.4
Xing Dai,Xiaohan Liu,Tao Xu,Chenjie Gu,Feiyu Zhao,Tao Jiang
Controlled aggregation of noble metal nanoparticles is incredibly crucial for obtaining reliable surface-enhanced Raman scattering (SERS) properties. Here, a rational agglomeration of large Ag nanowires (NWs) and small Ag nanocubes (NCs) was readily facilitated by simply utilizing the intrinsic viscosity of adhesive tape , leading to the generation of abundant electromagnetic “hotspots” around the numerous crossed nanotips and ultra-small nanogaps. After the molecular enrichment efficiency was further promoted by exploiting a hydrophilic-hydrophobic strategy, an ultra-low limit of detection as 8.8 × 10 −11 M was demonstrated for rhodamine 6 G. Benefiting from the high sensitivity of the SERS chip, its application in the determination of clinical sample containing mixed gefitinib and ritonavir was finally proved, facilitating impressive specificity and accuracy. Considering the simplicity and reliability, our strategy has great potentials to be applied in clinical blood drug identification and concentration monitoring.
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ANALYTICA CHIMICA ACTA
·
2022
·
IF 6.911
Jieyu Hu,Xingxing Yu,Xiumei Zhuang,Yan Sun,Junjie Wang,Haiting Ren,Shenghao Zhang,Yueshou Zhang,Hongxing Qiu,Yongjun Hu
The p53 gene is a known cancer marker. We report a novel protocol for the SERS tandem strategy to detect the p53 gene with high sensitivity. Herein, the click reaction between azide and alkyne was catalyzed by utilizing copper oxide nanoparticles (CuONPs), which were enriched by a T-DNA-triggered hybridization chain reaction (HCR). The T-DNA signal was amplified by establishing the correlation between the T-DNA signal and the concentration of CuONPs in a nonenzymatic isothermal environment. In contrast to other Raman reporters, we used alkynyl compounds as Raman reporters, which showed excellent characteristics in the Raman-silent region (1800–2800 cm −1 ). Therefore, the highly sensitive and highly selective SERS signals could be obtained in complex biological matrices. Due to utilizing multistep amplification strategies, including the nanoparticle-modified HCR polymer and “click” reaction, the limit of detection (LOD) and the limit of quantification (LOQ) of this sensor could be as low as 0.0174 pM and 0.0583 pM, respectively. The accuracy of the strategy expressed as the RSD was in the range of 3.14%–6.21%. The results indicated that the constructed sensor has excellent performance for the detection of the p53 gene in serum samples in a low concentration range, which suggests that the proposed enzyme-free SERS analytical sensor has good clinical application prospects.
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FOOD CONTROL
·
2023
·
IF 6
Yangjie Chen,Li Zheng,Juan Hao,Lijuan Huang,Qinghui Wang,Youting Zhou,Zhihang Yin,Lingpu Jia,Huiming Li,Wenlong Liao,Kunping Liu
In this study, a magnetic metal-organic frameworks (MOFs) based hybrid substrate was designed and fabricated for SERS detection of enrofloxacin (ENR) in milk. The magnetic MOF (Fe 3 O 4 @UiO-66-NH 2 ) was synthesized by a facile microwave-assisted method, then loaded with popcorn-like Au nanoparticles (NPs) through electrostatic interaction. The as-prepared SERS substrate (Fe 3 O 4 @UiO-66-NH 2 /Au NPs) integrated the abundant hot spots of popcorn-like Au NPs with porous Fe 3 O 4 @UiO-66-NH 2 , which greatly improved its SERS activity and stability. More importantly, benefiting from the fingerprint characteristics of SERS and adsorption performance of Fe 3 O 4 @UiO-66-NH 2 , the SERS substrate exhibited high selectivity and sensitivity in detecting ENR. Under the optimal detection condition, a good linearity (R 2 = 0.996) was obtained with ENR concentration of 1.0 × 10 −9 –1.0 × 10 −6 M in spiked milk, the LOD was as low as 5.26 × 10 −10 M, and the recoveries were in the range of 76.1–86.1 % at three different spiking levels. The whole detection process including sample pretreatment took about 50 min, which indicating that the prepared substrate has great potential in sensitive and real-time detection of ENR in milk.
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