NPG Asia Materials
·
2022
·Vol. 14
·期 1
·1-12
·
IF 10.761
Shen Peiyan,Li Mengru,Li Ran,Han Bo,Ma Haojie,Hou Xueyan,Zhang Yuqi,Wang Ji-Jiang
Smart photonic hydrogels based on two-dimensional photonic crystals (2DPC) provide a promising sensing platform for constructing novel chemical and biological sensors due to their facile optical signal readout and highly sensitive responsivity toward target analytes. Aptamers, as recognition elements with high selectivity and affinity, are extensively used to construct a variety of sensors. Herein, we developed two partially base complementary aptamer-functionalized 2DPC hydrogels as aptasensors for the detection of thrombin (TB) in human serum. The photonic hydrogel aptasensors swelled upon exposure to TB solution, leading to an increase in the particle spacing of the 2DPCs. The particle spacing changes were acquired by simply measuring the diameters of the Debye ring diffracted by the 2DPCs without the requirement of sophisticated instruments. The aptasensor swelling resulted from the decrease in the hydrogel cross-linking density induced by the specific binding between one of the aptamers and TB and the increase in hydrogel mixing free energy induced by the introduction of TB. The particle spacing increase of the optimized aptasensor was linear over the TB concentration range of 1–500 nM, and the limit of detection was 0.64 nM. The constructed 2DPC hydrogel aptasensor was used to detect TB in human serum and achieved recoveries of 95.74–104.21% and a relative standard deviation of 2.52–6.58%, showing the practicability and accuracy of the sensor. The aptamer-actuated 2DPC hydrogel biosensor provides a new strategy for designing other target molecule-sensitive aptasensors, showing great potential for development into home kits.
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Nature Communications
·
2025
·
IF 15.7
Xiao-Rui Ren,Baichuan Kou,Qing Hao,Francesco Bertocchi,Ying Xu,Lu Wang,Zhen-Lian Zhao,Ting Chen,Li-Jun Wan,Dong Wang
Engineering symmetry breaking is a fertile ground to develop innovative materials with fascinating properties. Herein, we introduce crystallographically incompatible rotation element into two-dimensional polymers and construct twist-stacked covalent organic framework nanofibers (abbreviated as t -COFs). These t -COFs are derived from polymerization between 2,4,6-triformylphloroglucinol and diamines modulated by monodentate alkylamine. Comprehensive electron microscopy analysis reveals their well-defined fibrous morphology and twist-stacking details. The time-dependent growth process study discloses a new radial growth pattern, which enables t -COFs to inherit twist-stacking from hexagonal macrocycle nanotube intermediate. Intriguingly, the twist-stacking mode is found to be intrinsic when using amine modulator, and enantiomeric bias towards one stacking direction can be achieved using chiral amine. Disrupting the inversion symmetry, the consecutive regular rotation of COF layers endows COF nanofibers with structural chirality and large chiroptical response. Twisted two-dimensional materials are materials with fascinating properties. Here, the authors report on the incorporation of a crystallographically incompatible rotation element into two dimensional polymers to construct twist-stacked covalent organic framework nanofibers with interesting chiroptical properties.
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MICROCHIMICA ACTA
·
2022
·Vol. 189
·期 11
·1-12
·
IF 6.408
Sha Zhou,Wang Chunnan,Ma Rui,Gao Xiaochun,Sun Shuqing
Uniform and monodisperse quantum dot (QD)–encoded magnetic microbeads with Janus structure were produced in a microfluidic device via photopolymerization. UV light through a microscope objective was used to solidify the microbeads which showed sharp interfaces and excellent magnetic responses. QDs with different emission peaks (450 nm for blue and 640 nm for red) were mixed at different ratios to provide three spectral codes. The QD-encoded microbeads can be distinguished by analyzing their fluorescent images in HSV color space. After hydrolysis of the anhydride group in alkaline solution, protein was immobilized on microbeads via activation of carboxyl groups using (1-ethyl-3(3-dimethylaminoprophyl) carbodiimide/N-hydroxysuccinimide (EDC/NHS). A microhole array in polydimethylsiloxane (PDMS) substrates with a specific size was fabricated to trap individual microbeads in a single microhole. The combination of Janus-structured QD-encoded magnetic microbeads and microhole arrays facilitates both flexibility, binding kinetics, sensitivity for suspension assay, and fluorescence mapping analysis for conventional biochips, thus providing a novel platform for multiplex bioanalysis. The capability of this integration for multiplex immunoassays was verified using three kinds of IgG and their corresponding anti-IgG. A detection limit of 0.07 ng/mL was achieved for human IgG, indicating practical applications in various fields. Graphical abstract
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Journal of Orthopaedic Surgery and Research
·
2023
·Vol. 18
·期 1
·1-12
·
IF 2.6
Su Hairong,Yang Yulan,Lv Wanchun,Li Xiaoli,Zhao Binxiu
Background Osteoporosis (OP) is a systemic skeletal disorder with increased bone fragility. Human bone marrow mesenchymal stem cells (hBMSCs) have multi-lineage differentiation ability, which may play important roles in osteoporosis. In this study, we aim to investigate the role of hBMSC-derived miR-382 in osteogenic differentiation.Methods The miRNA and mRNA expressions in peripheral blood monocytes between persons with high or low bone mineral density (BMD) were compared. Then we collected the hBMSC-secreted sEV and examined the dominant components. The over-expression of the miR-382 in MG63 cell and its progression of osteogenic differentiation were investigated by qRT-PCR, western blot and alizarin red staining. The interaction between miR-382 and SLIT2 was confirmed by dual-luciferase assay. The role of SLIT2 was also confirmed through up-regulation in MG63 cell, and the osteogenic differentiation-associated gene and protein were tested.Results According to bioinformatic analysis, a series of differential expressed genes between persons with high or low BMD were compared. After internalization of hBMSC-sEV in MG63 cells, we observed that the ability of osteogenic differentiation was significantly enhanced. Similarly, after up-regulation of miR-382 in MG63 cells, osteogenic differentiation was also promoted. According to the dual-luciferase assay, the targeting function of miR-382 in SLIT2 was demonstrated. Moreover, the benefits of hBMSC-sEV in osteogenesis were abrogated through up-regulation of SLIT2.Conclusion Our study provided evidence that miR-382-contained hBMSC-sEV held great promise in osteogenic differentiation in MG63 cells after internalization by targeting SLIT2, which can be served as molecular targets to develop effective therapy.
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MOLECULAR AND CELLULAR BIOCHEMISTRY
·
2021
·Vol. 476
·期 5
·2061-2073
·
IF 3.396
Wang Gang,Wang Hefeng,Zhang Lijuan,Guo Feng,Wu Xiangyu,Liu Yang
Studies have reported that miR-195-5p plays a role in the Hirschsprung disease (HSCR). Our previous work found GDNF family receptor alpha 4 (GFRA4) is also associated with HSCR. In this study, we focused on whether miR-195-5p induces the absence of enteric neurons and enteric neural crest in HSCR by regulating GFRA4. The expression levels of GFRA4 and miR-195-5p in colon tissues were evaluated by real-time PCR (RT-PCR) assay. We overexpressed GFRA4 or miR-195-5p in SH-SY5Y cells, the cell proliferation, cell cycle, apoptosis and invasion were subsequently investigated by CCK-8 assay, EdU staining, Flow cytometry analysis and Transwell assay, respectively. We also established the xenograft model to detect the effect of miR-195-5p on tumor growth and GFRA4 and p-RET expressions. GFRA4 expression was significantly downregulated in the HSCR colon tissues when compared with that in the control tissues. Overexpression of GFRA4 significantly promoted proliferation, invasion and cell cycle arrest, and inhibited apoptosis of SH-SY5Y cells. We also proved that GFRA4 is a direct target of miR-195-5p, and miR-195-5p inhibited proliferation, invasion, cell cycle arrest and differentiation, and accelerated apoptosis in SH-SY5Y cells which can be reversed by GFRA4 overexpression. Furthermore, we demonstrated that miR-195-5p suppressed tumor growth, and observably decreased GFRA4 and p-RET expressions. Our findings suggest that miR-195-5p plays an important role in the pathogenesis of HSCR. MiR-195-5p inhibited proliferation, invasion and cell cycle arrest, and accelerated apoptosis of nerve cells by targeting GFRA4.
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JOURNAL OF MATERIALS SCIENCE
·
2018
·Vol. 54
·期 3
·2535-2551
·
IF 2.993
Shen Hao,Song Jiayi,Yang Ye,Su Ping,Yang Yi
The exploration of new tactics for manufacturing artificial immobilized multi-enzyme systems based on enzyme cascades has recently attracted considerable interest because of the urgent need for multi-enzyme catalysts and the high cost of free enzymes. Because of the inevitable limitations of native enzymes such as instability and storage issues, the development of nanozyme–enzyme cascades is needed. A versatile strategy was developed for fabricating an efficient multi-catalyst system by immobilizing glucose oxidase (GO x ) on ferriferous oxide nanocomposites functionalized with nitrogen-doped graphene quantum dots (Fe 3 O 4 @N-GQDs) through DNA-directed immobilization. The Fe 3 O 4 @N-GQDs acted as a carrier for the natural enzyme and showed high peroxidase activity which enabled an enzyme cascade that included GO x to be set up. This multi-catalyst system showed great catalytic activity, reversibility and operational stability. The surfaces of GO x -targeted magnetic nanoparticles were regenerated by mild dehybridization of DNA. The Michaelis constant ( K m ) and maximum initial velocity ( V max ) of the multi-catalyst system were 1.069 mM and 11.2 × 10 −8 M s −1 , respectively, which are considerably better than the corresponding values for adsorbed and free bienzyme combinations. The increased bioactivity of the multi-catalyst system is ascribed to the satisfactory peroxidase-like activity of Fe 3 O 4 @N-GQDs, the enzyme–promoting effect of the QDs and enhancement by DNA-directed immobilization. Because of the diverse range of possible nanozyme–enzyme combinations and high efficiency of this approach, this work provides a novel pathway for the manufacturing of synthetic enzyme catalyst systems, which have great potential in the field of biotechnology.
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Scientific Reports
·
2020
·Vol. 10
·期 1
·1-11
·
IF 3.998
Chen Minjian,Yang Jing,Li Lei,Hu Yanhui,Lu Xiaomei,Sun Rongli,Wang Yubang,Wang Xinru,Zhang Xiaoling
Recently, celastrol has shown great potential for inducing apoptosis in acute myeloid leukemia cells, especially acute promyelocytic leukaemia cells. However, the mechanism is poorly understood. Metabolomics provides an overall understanding of metabolic mechanisms to illustrate celastrol's mechanism of action. We treated both nude mice bearing HL-60 cell xenografts in vivo and HL-60 cells as well as NB-4 cells in vitro with celastrol. Ultra-performance liquid chromatography coupled with mass spectrometry was used for metabolomics analysis of HL-60 cells in vivo and for targeted L-cysteine analysis in HL-60 and NB-4 cells in vitro . Flow cytometric analysis was performed to assess mitochondrial membrane potential, reactive oxygen species and apoptosis. Western blotting was conducted to detect the p53, Bax, cleaved caspase 9 and cleaved caspase 3 proteins. Celastrol inhibited tumour growth, induced apoptosis, and upregulated pro-apoptotic proteins in the xenograft tumour mouse model. Metabolomics showed that cysteine metabolism was the key metabolic alteration after celastrol treatment in HL-60 cells in vivo . Celastrol decreased L-cysteine in HL-60 cells. Acetylcysteine supplementation reversed reactive oxygen species accumulation and apoptosis induced by celastrol and reversed the dramatic decrease in the mitochondrial membrane potential and upregulation of pro-apoptotic proteins in HL-60 cells. In NB-4 cells, celastrol decreased L-cysteine, and acetylcysteine reversed celastrol-induced reactive oxygen species accumulation and apoptosis. We are the first to identify the involvement of a cysteine metabolism/reactive oxygen species/p53/Bax/caspase 9/caspase 3 pathway in celastrol-triggered mitochondrial apoptosis in HL-60 and NB-4 cells, providing a novel underlying mechanism through which celastrol could be used to treat acute myeloid leukaemia, especially acute promyelocytic leukaemia.
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JOURNAL OF WUHAN UNIVERSITY OF TECHNOLOGY-MATERIALS SCIENCE EDITION
·
2021
·Vol. 36
·期 6
·934-941
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IF 0.957
Yan Chunchao,Xiao Feng,Qiu Tong,Zhang Xueqiong,Zhan Siwen,Lu Mengli,Yang Mengjia,Zhang Juan
To synthesize three different grafting ratios of gallic acid (GA)-chitosan (CS) copolymer by a free radical mediated grafting method, which is further applied to the field of antibacterial materials, crosslinking structures of the compound GA-CS copolymer were characterized, fully indicating that gallic acid is resoundingly grafted onto chitosan. The grafting ratios of three copolymers GA-CS are 45.71% (I), 36.12% (II), and 18.96% (III) were determined by UV-Vis spectrophotometer. The minimum inhibitory concentrations of three GA-CS copolymers are 30 µg/mL against Escherichia coli and are ranged from 250 to 550 µg/mL against Staphylococcus aureus. By counting viable bacterial colonies, it can be found that antibacterial property is preferable by increasing the grafting ratio of GA-CS copolymers. Findings of investigation on aforementioned bacteria experiment indicate that the CFU/mL values of GA-CS (I, II, III) are 2.04×10 6 , 7.56×10 6 , 1.48×10 7 to Staphylococcus aureus , and 2.96×10 6 , 1.01×10 7 , 2.14×10 7 to Escherichia coli after 12 h treatment. In addition, the interaction process between GA-CS copolymer and bacteria can be observed through a transmission electron microscope. The specific manifestation is that the bacterial cell membranes are ruptured after being treated with the copolymer, which causes the cell contents to flow out, and the cell morphology is shrunk and out of shape.
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Nanoscale Research Letters
·
2018
·Vol. 13
·期 1
·1-10
·
IF 3.125
Zhang Shiyu,Zhang Chao,Liu Mingyue,Huang Renliang,Su Rongxin,Qi Wei,He Zhimin
Nanoscale zero-valent iron (nZVI) has shown considerable promise in the treatment of chlorinated organic compounds, but rapid aggregation and inactivation hinder its application. In this study, palladium-doped zero-valent iron nanoparticles involving poly (γ-glutamic acid) (Fe-Pd@PGA NPs) were synthesized. The nanoparticles were small (~100 nm), uniformly distributed, and highly stable. The dechlorination performance of Fe-Pd@PGA NPs was evaluated using p -CP as a model. The results demonstrated that Fe-Pd@PGA NPs show high activity even in weakly alkaline conditions. The maximum rate constant reached 0.331 min − 1 at pH 9.0 with a Fe to p -CP ratio of 100. Additionally, the dechlorination activity of Fe-Pd@PGA NPs is more than ten times higher than that of the bare Fe-Pd NPs, demonstrating the crucial role of PGA in this system. Furthermore, we investigated the dechlorination performance in the presence of different anions. The results indicated that Fe-Pd@PGA NPs can maintain high activity in the presence of Cl − , H 2 PO 4 − , and humic acid, while HPO 4 2− and HCO 3 − ions slightly reduce the dechlorination activity. We believed that PGA is a promising stabilizer and promoter for Fe-Pd NPs and the Fe-Pd@PGA NPs have the potential for practical applications.
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JOURNAL OF MATERIALS SCIENCE-MATERIALS IN MEDICINE
·
2018
·Vol. 29
·期 8
·1-14
·
IF 2.448
Liu Chundong,Wang Jianhua,Huang Sheng,Yu Lin,Wang Yan,Chen Hang,Wang Dong
Peptide nucleic acid (PNA) holds enormous potentials as antisense/antigenic drug due to its specific binding ability and biostability with DNA or RNA. However, the poor cellular delivery is the key obstacle in development of PNA therapy. To overcome this difficulty, we developed self-assembled nanoparticles (NPs) for delivery of PNA to living cells using amphiphilic CS derivatives. A series of N,N,N-trimethyl-O-alkyl chitosans (TMACs) with different lengths of alkyl chains were synthesized. The structures of these synthesized chemicals were characterized with FT-IR and 1 H NMR. We found that the TMACs were all able to self-assemble in aqueous condition to form nano-size NPs. These nano-size NPs are spherical shape with a size range of around 100 nm and a zeta potential above +30 mV. PNA was easily encapsulated into chitosan derivative NPs by an ultrasonic method with entrapment efficiency up to 75%. The PNA-loaded TMAC NPs released the drug in a sustained manner in PBS (pH 7.4) at 37 °C. N,N,N-trimethyl-O-cetyl chitosan (TMCC) showed the best in vitro hemocompatibility and cell viability. These TMCC based NPs were able to dramatically increase the cellular uptake of PNA, specifically, 66-fold higher compared to without using these nanoparticles. The results suggest that the designed TMCC NPs might be a promising solution for improving cellular delivery of PNA.
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JOURNAL OF POLYMERS AND THE ENVIRONMENT
·
2022
·Vol. 30
·期 11
·4550-4561
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IF 4.705
Jiang Longwei,Wang Fenghui,Xie Cancan,Xie Xinyan,Meng Xiangyi,Zhang Huajiang
In this study, active packaging films were developed by incorporating nano TiO 2 and pomegranate peel extract (PPE) into high-amylose corn starch (HCS)/konjac glucomannan (KG) matrix for the first time. The aim of present work was to investigate the influence of nano TiO 2 and PPE on physicochemical and functional properties of HCS/KG-based films. The films were characterized by XRD, FT-IR and SEM, and the tensile, antibacterial and antioxidant properties were evaluated. The results showed that the crystallinity of the composite films was increased and the microstructure was more uniform and dense after adding TiO 2 and PPE, and the intermolecular hydrogen bonds were formed between TiO 2 , PPE, and film matrix. Incorporation of TiO 2 and PPE significantly improved tensile properties and decreased water solubility and water vapor permeability of the composite films (p < 0.05). HCS/KG films incorporated with TiO 2 and PPE presented remarkable antibacterial activity against Escherichia coli and Staphylococcus aureus , and exhibited strong antioxidant activity due to the polyphenol compounds in PPE. All films properties not only changed with the content of TiO 2 and PPE, but also improved synergistically when they were added together. The developed composite films were used as coating for the preservation of Agaricus bisporus , and the weight, firmness and total soluble solids of A. bisporus were significantly maintained and browning was effectively inhibited during storage. Therefore, HCS/KG composite film/coating containing TiO 2 and PPE has great potential as an attractive commercialization technology to ensure the quality and extend the shelf life of foods.
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ENVIRONMENTAL SCIENCE AND POLLUTION RESEARCH
·
2018
·Vol. 25
·期 15
·14656-14665
·
IF 2.8
Dai Shixiang,Zhao Ling,Teng Ying,Wang Xiaomi,Ren Wenjie
The fate and transport of polychlorinated biphenyls (PCBs), a class of persistent organic compounds, in soils was markedly affected by their sorption/desorption on soil organic matters (SOM) due to high hydrophobicity of PCBs. Humic acid (HA), an important fraction of SOM, has no steady composition and microstructure from different origins, resulting in their diverse sorption capacity. Therefore, the effect of composition and microstructure of HA on sorption of 3,3′,4,4′-tetrachlorobiphenyl (PCB77) was investigated in this study. The primary sorption mechanism of PCB77 on HAs was also demonstrated by using Fourier transform infrared (FTIR) and solid-state carbon-13 nuclear magnetic resonance ( 13 C-NMR) spectroscopy analysis. Elemental content analysis of three HAs showed that the content of unsaturated C (the total of aromatic, carboxylic, and carbonyl carbon) followed an order of YHA>SHA>AHA. Furthermore, YHA and SHA had remarkably rough and complicated particle surfaces but AHA had relatively smooth surface and the well-proportioned and interspersed particle sizes. The results of the sorption experiment showed that the sorption capacity of PCB77 on HAs followed a similar order of YHA>SHA>AHA, indicating the content of unsaturated C of HAs controlled the sorption of PCB77 on HAs. Sorption of PCB77 on either AHA or SHA did not change with increasing ionic strength of background solution, implying that there was no H-bond or electrostatic interaction between PCB77 and HAs. The result of FTIR and 13 C-NMR spectra showed the primarily possible mechanism was π-π conjugative interaction and hydrophobic binding between PCB77 and HAs.
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