INTERNATIONAL JOURNAL OF BIOLOGICAL MACROMOLECULES
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2025
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IF 8.5
Huan Liu,Aminov Nail,Decheng Meng,Liran Zhu,Xiaohan Guo,Cong Li,Huanjun Li
Dissolving microneedles (DMNs) represent promising platforms for painless transdermal drug delivery and biosensing, yet integrating multifunctionality with sustainable recyclability via photo-curable additive manufacturing strategy remains challenging. Here we report a multifunctional, pioneering 3D-printed DMNs patch combining a ternary polymerizable deep eutectic solvent (PDES) system with a recyclable eutectogel backing layer. DMNs, formulated with biocompatible choline chloride, itaconic acid, and N-vinyl-2-pyrrolidone, enable rapid photopolymerization and excellent mechanical strength for effective skin penetration. Chitosan-based glucose-responsive nanoparticles incorporated into the DMNs facilitate controlled and responsive drug release, demonstrating in diabetic rats a rapid blood glucose reduction to 18.7 % of initial levels within 3 h post-administration, sustained for 4 h while minimizing hypoglycemic risk. The conductive, adhesive, and antibacterial eutectogel backing layer offers superior properties for wound dressing and wearable sensors, also serving as a real-time health monitor for human movements. In diabetic mouse models, the CHPG-TA@CUR-DMN@NPs system achieved 68.08 % wound closure by day 10, significantly accelerating healing with reduced inflammation and scarring. The backing layer is designed for easy dissolution and recycling after use, highlighting the eco-friendly and cost-effective nature of our all-in-one integrated multi-material DMNs platform.
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高影响因子
Acta Biomaterialia
·
2024
·
IF 9.4
Kaidi Ding,Wenlong Cong,Ying Liu,Changtong Song,Haoyang Mi,Chuntai Liu,Yuhong Ma,Changyu Shen
Endoscopic surgery is an effective and common clinical practice for chronic sinusitis. Nasal packing materials are applied in nasal surgery to prevent hemorrhage and promote wound healing. In this study, a degradable polyurethane foam dressing is successfully developed as a promising nasal packing material with good biocompatibility and antibacterial capability. Specifically, quaternized chitosan (QCS) serves as the crosslinker instead of polyols to offer polyurethane foam (PUF-QCS) antibacterial capability. The PUF-QCS2.0 % (with 2.0 wt% QCS) exhibits satisfactory liquid absorption capacity (19.4 g/g), high compressive strengths at both wet (14.5 kPa) and dry states (7.7 kPa), and a good degradation rate (8.3 %) within 7 days. Meanwhile, PUF-QCS2.0 % retains long-term antibacterial activity for 7 days and kills 97.3 % of S. aureus and 91.8 % of E. coli within 6 hours in antibacterial testing. Furthermore, PUF-QCS2.0 % demonstrates a positive hemostatic response in the rabbit nasal septum mucosa trauma model by reducing hemostatic time over 50.0 % and decreasing blood loss up to 76.1 % compared to the commercial PVA nasal packing sponge. Importantly, PUF-QCS also exhibits a significant antibacterial activity in nasal cavity. This nasal packing material has advantages in post-surgery bleeding control and infection prevention. Statement of Significance The performance of a nasal packing sponge requires good mechanical properties, fast and high liquid absorption rate, effective degradability and strong antibacterial activity. These features are helpful for improving the postoperative recovery and patient healing. However, integrating these into a single polyurethane foam is a challenge. In this study, quaternized chitosan (QCS) is synthesized and used as a chain extender and antibacterial agent in preparing a degradable polyurethane foam (PUF-QCS) dressing. PUF-QCS undergoes partial degradation and exhibits effective broad-spectrum antibacterial activity in 7 days. The reduction of postoperative bleeding and infection observed in the animal experiment further demonstrates that the PUF-QCS developed here outperforms the existing commercial nasal packing materials.
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INTERNATIONAL JOURNAL OF BIOLOGICAL MACROMOLECULES
·
2024
·
IF 7.7
Xubing Yang,Wei Liu,Pengfei Han,Yin You,Ju Lv,Xinyue Zhang,Ziyu Qin,Xueqiong Yin
Uranium recovery from wastewater or seawater is important for both pollution control and uranium supply. Due to the complexity of the water body, it requires that the adsorbent should not only be highly efficient for selective adsorption but also have good antimicrobial properties. In this study, an antimicrobial thermosensitive hydrogel (UITAC) for uranium adsorption was prepared by one-step ion-imprinted polymerization using chitosan as a substrate and allyl trimethylammonium chloride as the antimicrobial modifier. UITAC showed excellent antibacterial rate against Escherichia coli and Staphylococcus aureus , being 98.8 % and 89.1 %, respectively. Endothermic and exothermic peaks respectively showed up at 36.3–38.5 °C and 30.5–34.1 °C in the DSC curves. UITAC quickly achieved its adsorption equilibrium in 30.0 min at 50 °C, pH 5.0 in the 0.8 mg/mL UO 2 2+ solution, with an adsorption capacity of 81.2 mg/g. The adsorption capacity could remain at 80 % after 5 cycles of repeated use. UITAC showed better adsorption selectivity to UO 2 2+ than vanadium and other metal ions, with selectivity coefficients α(UO 2 2+ /M n+ ) being 1.4–10.3. The pseudo-second-order kinetics and Langmuir adsorption model had a better fit for UO 2 2+ adsorption by UITAC. The adsorption was a spontaneous process. The Gibbs Free Energy change, enthalpy change, and entropy change at 323.2 K were − 16.0 kJ/mol, 64.3 kJ/mol, and 248.4 J/mol·K, respectively. UITAC showed high potential in practical application environment.
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Journal of Orthopaedic Translation
·
2024
·
IF 5.9
Peng He,Yanbin Zhao,Bin Wang,Guoyin Liu,Lei Zhang,Mei Li,Bin Xu,Weihua Cai,Chenglin Chu,Yu Cong
Background The repair of bone defects has always been a significant challenge in clinical medicine. To address this challenge, doctors often utilize autologous bone grafts, allogeneic bone grafts and artificial bone substitutes. However, the former two methods may result in additional trauma and complications, while allogeneic bone grafts carry the risks of immune rejection and disease transmission. Magnesium phosphate cement (MPC), as a artificial bone substitutes, has been a potential biomaterial for repairing bone defects, but its clinical application is limited by insufficient mechanical strength and poor osteoinductive activity. Methods In this study, the cement liquid phase base on rhBMP-2 and chitosan solution into MPC were obtained and investigated. After mixing with a cement liquid, the structural and phase composition, morphology, chemical structure, setting time, compressive strength, degradation behavior, solubility, and cellular responses and bone regeneration in response to CHI-rhBMP2 MPC were investigated in vitro and in vivo. Results After the chemical component modification, CHI-rhBMP2 MPC possessed controllable degradation rate, moderate setting time, appropriate cuing temperature, good injectability, and improved initial strength. In vitro tests showed that the CHIrhBMP2 MPC could promote cell proliferation and adhesion, as well as that contribute to osteoblast differentiation and mineralization. In addition, cement materials were implanted into the rabbit femoral condyles for in vivo osseointegration evaluation. The results displayed that more new bone grew around CHI-rhBMP2 MPC, verifying improved osseointegration capacity. Transcriptome analysis revealed that focal adhesion, Forkhead box O（FoxO） signaling pathway and P13K/AKT signaling pathway were may involved in CHI-rhBMP2 MPC induced new bone formation. Conclusion This work provides a new strategy for the rational design of potential bone repair candidate materials.
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INTERNATIONAL JOURNAL OF BIOLOGICAL MACROMOLECULES
·
2024
·
IF 7.7
Xiumei Zhang,Miao Qin,Jinqiao Jia,Akhlaq Ahmed,Liqin Zhao,Weiwei Lan,Yan Wei,Ziwei Liang,Xudong Ma,Yiwei Shi,Di Huang
Adhesive hydrogels have been widely studied as wound dressings due to their excellent biocompatibility and biological activity. However, most designed hydrogels still exist limitations including potentially toxic monomer, complex preparation process and non-degradable property. Here, a natural and degradable gelatin/casein hydrogel was prepared by enzymatic cross-linking. The hydrogel could adhere to tissue on-demand with the mediation of chitosan (CS) solution. It was found that the adhesion strength of hydrogel could be controlled by adjusting gelatin/casein ratio, EDC&NHS concentration, CS concentration, glycerol content and crosslinking degree. To further expand the applicability of hydrogels, the degradation and drug release rate of hydrogels could be modulated by changing transglutaminase (TG) concentration. Moreover, tetracycline hydrochloride (TH) was loaded into hydrogel as a drug model, which endowed hydrogel with good antibacterial properties. It was shown that the 0.03 % TH hydrogel had excellent blood compatibility and cell compatibility, and can promote the healing of infected wounds in mice. This research provides a new natural adhesive hydrogel for biomedical engineering field.
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CARBOHYDRATE POLYMERS
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2010
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IF 3.167
Tao Xu,Meihua Xin,Mingchun Li,Huili Huang,Shengquan Zhou
O -Methyl free N , N , N -trimethyl chitosan (TMC) was synthesized by treating chitosan with formic acid and formaldehyde firstly, followed by methylation with CH 3 I. TMC was further carboxymethylated by monochloroacetic acid to obtain N , N , N -trimethyl- O -carboxymethyl chitosan (TMCMC). The products were characterized by FTIR, 1 H NMR, EA and TGA. Their antibacterial activity was investigated against Staphylococcus aureus and Escherichia coli . The antibacterial activity of TMC decreased as the degree of substitution increased at pH 5.5. But the structure activity relationship was reversed at pH 7.2. TMCMC acted weaker than TMC, and its activity decreased as the degree of carboxymethylation increased. The experimental results showed that the activity of N , N , N -trimethyl amino group was weaker than other non-quaternized amino groups, and carboxymethylation didnot enhance the antibacterial activity directly.
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COMPOSITES PART B-ENGINEERING
·
2024
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IF 12.7
Ying Han,Mouyuan Sun,Xingchen Lu,Kailei Xu,Mengfei Yu,Huayong Yang,Jun Yin
In neural tissue engineering , biomaterial scaffolds that have high conductivity and customized structures are crucial in promoting nerve regeneration. Poly(3,4-ethylenedioxythiophene) (PEDOT) has emerged as a promising conductive polymer with excellent chemical stability and biocompatibility. However, traditional three-dimensional (3D) printing of PEDOT-based conductive scaffolds faces challenges in limited printing resolution, poor solubility, and brittleness of conductive materials . Herein, digital light processing (DLP) printing was used to fabricate complex hydrogel structures using gelatin methacryloyl (GelMA) and chitosan (CS) while incorporating PEDOT nanoparticles through interfacial polymerization to create conducting pathways within a hydrogel structure. The integration of PEDOT significantly enhanced the electrical conductivity and mechanical properties of the GelMA/CS hydrogel while preserving printed details. The GelMA/CS-PEDOT hydrogel promoted cell proliferation and facilitated axon outgrowth of PC12 cells and Schwann cells during in vitro culture. Moreover, in vitro direct current electrical stimulation promoted axon elongation of PC12 cells cultured on a conductive substrate. In vivo studies used a conductive nerve conduit to repair a 10-mm rat sciatic nerve defect, validating the efficacy of GelMA/CS-PEDOT scaffold in peripheral nerve injury repair. These findings highlight the significant potential of conductive GelMA/CS-PEDOT hydrogel in the field of neural tissue engineering.
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Materials Science & Engineering C-Materials for Biological Applications
·
2017
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IF 4.164
Tian-Ming Liu,Jing-Jie Xu,Yun-Ren Qiu
In order to ameliorate the biocompatibility of polysulfone (PSf), sulfonated hydroxypropyl chitosan (SHPCS) was grafted from PSf membrane material by Schiff-Base reaction. The original and modified membranes were characterized by attenuated total reflectance-Fourier transform infrared spectroscope (ATR-FTIR), X-ray photoelectron spectroscopy (XPS), scanning electron microscopy (SEM), water contact angle (WCA) measurement, tensile strength test and antibacterial test in vitro, and the results indicated that the PSf modified by SHPCS (PSf-SHPCS) was synthesized successfully, the hydrophilicity of PSf-SHPCS membrane was improved to a great extent, all the membranes possessed good stability in physiological condition and the PSf-SHPCS membrane had good antibacterial property. Protein adsorption, platelet adhesion, hemolysis assay, plasma recalcification time, activated partial thromboplastin time (APTT), prothrombin time (PT), thrombin time (TT) and whole blood clotting time were executed to evaluate the hemocompatibility of membranes decorated by SHPCS, and the results demonstrated that the modified membrane had fine hemocompatibility.
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JOURNAL OF APPLIED POLYMER SCIENCE
·
2017
·Vol. 134
·期 34
·45229
·
IF 1.86
Chunyang Shang,Yanjun Chen,Chaocan Zhang,Zongyi Deng,Zhihui Wu
This article describes a new and simple method to control the release of payloads encapsulated in chitosan capsules. The capsules with the liquid core and rigid shell are performed by drop-wisely adding chitosan solution into anionic surfactant solution. The capsules can be destroyed by β-CD because β-CD binds and sequesters surfactant from the shell through the host-guest interaction. The destruction process is studied by optical microscopy and scanning electron microscopy. The capsules can swell in acid solution and shrink in alkaline solution. It is found that the destruction speed increases with increasing acidity or alkalinity of β-CD solution. Rhodamine B encapsulated in the liquid-core of chitosan capsules can be released from the capsules in β-CD solution. The release rate can be adjusted by the pH value of β-CD solution. The release speed of the capsule increases with the increase of acidity or alkalinity in β-CD solution. The above concept can provide more versatility for controlled release of a variety of payloads such as drugs, cosmetics, and so on. © 2017 Wiley Periodicals, Inc. J. Appl. Polym. Sci. 2017 , 134 , 45229.
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INTERNATIONAL JOURNAL OF BIOLOGICAL MACROMOLECULES
·
2024
·
IF 7.7
Xiaoyu Wu,Chuanbo Ding,Xinglong Liu,Qiteng Ding,Shuai Zhang,Yue Wang,Chenran Xin,Hewei Wei,Rui Mao,Guiping Zhang,Ting Zhao,Wencong Liu
Wound healing in diabetic patients is often complicated by issues like inflammation, infection, bleeding, and fluid retention. To tackle these challenges, it is essential to create hydrogel dressings with anti-inflammatory, antibacterial, and antioxidative properties. This study aimed to synthesize Phlorizin-Liposomes (PL) through the thin-film dispersion method and integrate them into an oxidized sodium alginate (OSA) and carboxymethyl chitosan (CMCS) hydrogel scaffold, resulting in an OSA/CMCS/PL (PLOCS) composite hydrogel via a Schiff base reaction. Characterization of the composite was performed using FTIR, TEM, and SEM techniques. The research assessed the swelling behavior, antibacterial effectiveness, and biocompatibility of the PLOCS composite hydrogel, while also investigating how PLOCS facilitates diabetic wound healing. The results demonstrated that PLOCS effectively controls drug release, possesses favorable swelling and degradation characteristics, and shows significant antioxidative properties along with in vitro biocompatibility. Histological analysis confirmed that PLOCS supports the proliferation of healthy epithelial tissue and collagen production. Western blotting indicated that PLOCS diminishes inflammation by inhibiting the TLR4/NF-κB/MyD88 pathway and activates Nrf2 to boost wound healing, increasing the levels of antioxidative enzymes such as HO-1, NQO1, and GCLC. In summary, PLOCS presents a promising new option for advanced wound dressings aimed at treating diabetic ulcers.
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CARBOHYDRATE POLYMERS
·
2024
·
IF 10.7
Haihua Luo,Huan Xu,Hongli Zhang,Xiangming Li,Qiong Wu,Tian Gao
Drug-resistant bacterial infections represent a critical global public health challenge, driven largely by the misuse and overuse of antibiotics. Tackling the growing threat of bacterial resistance necessitates the development of innovative antibacterial agents that function independently of traditional antibiotics. In this study, novel antibacterial nano-micelles were rationally designed by conjugating quaternized chitosan with the photosensitizer chlorin e6. These nano-micelles promoted the solubility and stability of chlorin e6 while maintaining robust singlet oxygen generation under 660 nm laser irradiation. The positively charged nano-micelles facilitated strong electrostatic interactions with bacterial surfaces, promoting efficient adhesion and enabling effective photodynamic antibacterial activity mediated by singlet oxygen. In vitro experiments revealed that the nano-micelles exhibited instant and prolonged antibacterial effects, effectively suppressing bacterial proliferation without inducing resistance and disrupting mature biofilms. Furthermore, in conjunction with laser treatment, nano-micelles exhibited remarkable in vivo antibacterial efficacy, significantly accelerating the healing of skin wounds infected with Methicillin-resistant Staphylococcus aureus while maintaining favorable biocompatibility. These findings highlight the potential of the nano-micelles as a promising non-antibiotic antibacterial formulation, offering a powerful strategy to combat drug-resistant bacterial infections and paving the way for their clinical application in infection management.
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COLLOIDS AND SURFACES B-BIOINTERFACES
·
2024
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IF 5.4
Li Li,Fengting Xiang,Fan Wang,Anqi Chen,Yu Liu
The current situation of diabetes prevention and control is extremely severe. For instance, glimepiride (GLM), a third-generation sulfonylurea, demonstrates suboptimal clinical efficacy in oral dosage forms, which underscores the pressing need for the development of a new dosage form. Recently, in situ gel subcutaneous implants have garnered considerable attention. Hydroxybutyl chitosan (HBC) can spontaneously crosslink to form a thermosensitive hydrogel and has good biocompatibility. However, its application is hindered by its limited mechanical properties. Graphene oxide (GO), known for its stable dispersion in water, can load GLM through π–π stacking interactions. When combined with HBC, GO enhances the mechanical properties and stability of the hydrogel. Therefore, an HBC–GO@GLM hydrogel was prepared. Rheological analysis revealed that the incorporation of GO increased the critical gelation temperature of the 5 wt% HBC hydrogel from 19.1°C to 27.2°C, considerably enhancing the mechanical properties of the hydrogel. Using encapsulation efficiency as an evaluation index, the optimal encapsulation efficiency of GO@GLM was determined to be 73.53% ± 0.45% with a drug loading capacity of 27.39 ± 0.17% using the Box–Behnken design model. Computer simulation technology validated the interaction between the materials and the drug release mechanism. Pharmacokinetic results showed that compared to the HBC@GLM group, the half-life (t1/2), mean residence time and the area under the curve for the HBC–GO@GLM group were approximately 3 times those of the HBC@GLM group. Subcutaneous implantation of the HBC–GO@GLM hydrogel for drug delivery considerably extended the drug’s action time in the body, thereby maintaining blood sugar levels within a normal and stable range for an extended period.
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