TALANTA
·
2017
·
IF 4.162
Zhong Feng Gao,Asmerom Yohannes Ogbe,Ei Ei Sann,Xudong Wang,Fan Xia
Monitoring blood glucose has attracted considerable attention because diabetes mellitus is a global public health problem. Herein, we reported a turn-on fluorescence detection strategy based on manganese dioxide (MnO 2 )-phenol formaldehyde resin (PFR) nanocomposite for rapid, sensitive, and selective detection of glucose levels in human blood. In this biosensing system, MnO 2 nanoshell on the PFR nanoparticle surfaces serve as a quencher. PFR fluorescence can make a recovery in the presence of H 2 O 2 , reducing MnO 2 to Mn 2+ . The sensor shows a linear range from 50 nM to 90 μM with a low detection limit of 20 nM for H 2 O 2 detection. Thus, the glucose can be detected on the basis of the enzymatic conversion of glucose by glucose oxidase to produce H 2 O 2 . This method exhibits a wide linear range from 5 μM to 1 mM with a low detection limit of 1.5 μM. Because of the excellent photostability offered by PFR, the developed strategy has been successfully applied for the diagnosis of diabetes mellitus in human blood samples. Compared with commercial glucometer, our method showed satisfactory results, indicating the significant reliability. The developed turn-on fluorescent sensor might hold great promise in nanomedicine and bioanalysis .
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高影响因子
CHROMATOGRAPHIA
·
2018
·Vol. 81
·期 4
·657-667
·
IF 1.401
Yu Guo-Wei,Cheng Qiang,Nie Jing,Wang Xia-Jun,Wang Peng,Li Zu-Guang,Lee Maw-Rong
Abstract Deep eutectic solvent has been gaining much attention in recent years due to its various novel properties. The present study develops a novel microwave-assisted hydrodistillation method based on pretreatment using deep eutectic solvent, for the extraction of essential oil (EO) from dry fruits of Amomum kravanh , Amomum tsaoko, and Amomum villosum , respectively. The process is optimized by single-factor experiments taking the improvement in the essential oil yield of A. kravanh as the target. The results indicate that using the reaction product of choline chloride and ethylene glycol as the deep eutectic solvent with 7:1 of mass ratio to fruit powder, the optimal conditions are (1) pretreatment stage: 500 W of microwave power, 50 °C of temperature, and 7 min of duration; (2) fast heating stage: 600 W of microwave power, 110 °C of temperature, and 5 min of duration; (3) hydrodistillation stage: 300 W of microwave power, 110 °C of temperature, and 30 min of duration. Under these conditions, the EO yields of A. kravanh , A. tsaoko, and A. villosum are 3.64, 2.16, and 1.62%, respectively. The EOs are analyzed by gas chromatography–mass spectrometry, with totally 63 compounds identified. In most cases, the new method brings about more identified compounds than the traditional methods. Moreover, the relative contents of hydrocarbons in most essential oils are improved by applying the new method. Thus, the approach based on deep eutectic solvent has been proved superior in the enhancement of essential oil production. Graphical abstract
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Microorganisms
·
2024
·Vol. 12
·期 8
·1526
·
IF 4.1
Jianzhi Zhao,Yuping Zhao,Longhao Wu,Ning Yan,Shuo Yang,Lili Xu,Deyun He,Hongxing Li,Xiaoming Bao
The economical and efficient commercial production of second-generation bioethanol requires fermentation microorganisms capable of entirely and rapidly utilizing all sugars in lignocellulosic hydrolysates. In this study, we developed a recombinantSaccharomyces cerevisiaestrain, BLH510, through protoplast fusion and metabolic engineering to enhance its ability to co-ferment glucose, xylose, cellobiose, and xylooligosaccharides while tolerating various inhibitors commonly found in lignocellulosic hydrolysates. The parental strains, LF1 and BLN26, were selected for their superior glucose/xylose co-fermentation capabilities and inhibitor tolerance, respectively. The fusion strain BLH510 demonstrated efficient utilization of mixed sugars and high ethanol yield under oxygen-limited conditions. Under low inoculum conditions, strain BLH510 could completely consume all four kinds of sugars in the medium within 84 h. The fermentation produced 33.96 g/L ethanol, achieving 84.3% of the theoretical ethanol yield. Despite the challenging presence of mixed inhibitors, BLH510 successfully metabolized all four sugars above after 120 h of fermentation, producing approximately 30 g/L ethanol and reaching 83% of the theoretical yield. Also, strain BLH510 exhibited increased intracellular trehalose content, particularly under conditions with mixed inhibitors, where the intracellular trehalose reached 239.3 mg/g yeast biomass. This elevated trehalose content contributes to the enhanced stress tolerance of BLH510. The study also optimized conditions for protoplast preparation and fusion, balancing high preparation efficiency and satisfactory regeneration efficiency. The results indicate that BLH510 is a promising candidate for industrial second-generation bioethanol production from lignocellulosic biomass, offering improved performance under challenging fermentation conditions. Our work demonstrates the potential of combining protoplast fusion and metabolic engineering to develop superiorS. cerevisiaestrains for lignocellulosic bioethanol production. This approach can also be extended to develop robust microbial platforms for producing a wide array of lignocellulosic biomass-based biochemicals.
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高影响因子
ENERGY
·
2024
·
IF 9
Bingbing Qiu,Jicheng Shi,Wei Hu,Yanfang Wang,Donghui Zhang,Huaqiang Chu
Hydrothermal liquefication of biomass to produce high-quality chemicals and liquid fuels is considered as a renewable and sustainable technology. Herein, a one-step hydrothermal method was used to prepare microcrystalline cellulose supported carbon-based catalyst PTA-BC with the activation factor of phosphotungstic acid. It was demonstrated by Py-FTIR, NH 3 -TPD and XPS that PTA-BC is not only rich in functional groups but also contains Brønsted acid sites (hydroxyl groups) and Lewis acid sites (C C, carboxyl groups). After experimental optimization, the optimum yield of furfural obtained from xylose catalyzed by PTA-BC was 81.02% at the reaction temperature of 180 °C and the reaction time of 20 min. The results show that PTA-BC contains –COOH, –OH and C C as catalytic sites and that the synergistic effect of these functional groups. The nature of the reaction solvent, appropriate reaction temperature and residence time are the main reasons for the good catalytic performance. This work provides a promising way for the synthesis of solid acid catalyst, which has a wide application prospect for the production of furfural.
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ULTRASONICS SONOCHEMISTRY
·
2025
·
IF 9.7
Xinxin Yu,Ke Zhu,Faguang Hu,Rongsuo Hu,Wenjiang Dong
Coffee peel (CP) is an important by-product of coffee processing which is rich in bioactive components. In the present work, we extracted coffee peel soluble dietary fiber (CSDF) via ultrasound-assisted alcohol precipitation to reduce resource waste as well as environmental pollution and evaluate its structure, monosaccharide composition, and functional and physicochemical characters. Based on our findings, ultrasonic treatment decreased particle size of CSDF from 131.49 ± 7.67 μm to 75.84 ± 1.20 μm, and markedly improved the homogeneity. The ultrasonic power of 200–300 W resulted in increased thermal stability due to a higher crystallinity of the CSDF. Increasing the ultrasonic power (0–500 W) reduced rhamnose level from 8.25 % to 7.61 % ( p < 0.05) and the SDF treated with 100 W of ultrasonic power had the highest fructose content (21.37 %). The ultrasonic treatment improved the water-solubility, oil-holding capacities and water-holding capacities of CSDF, with highest water-holding capacity being obtained at 300 W (8.92 ± 1.57 g/g). Additionally, the glucose and nitrite ion adsorption capacities significantly improved after ultrasonic modification. These findings may expand the application of CSDF in the food industry.
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CHEMICAL ENGINEERING JOURNAL
·
2024
·
IF 13.3
Xichuang Cheng,Chi Feng,Zhiqiang Li,Weiwei Zhang,Li Ji,Kun Wang,Jianxin Jiang
Lignocellulosic biomass, being a renewable carbon-based resource, holds paramount practical significance in terms of its comprehensive conversion and utilization for effectively substituting petroleum-based energy sources. Herein, we present a sustainable and environmental-friendly vanillic acid (VA) pretreatment approach for efficient and comprehensive utilization of sugarcane bagasse (SCB). The xylo -oligosaccharides (XOS) DP2-6 yield of 60.4 % (wt.% of xylan) was achieved under the conditions of 180 °C/15 min/6% VA (wt.% of SCB), while lignin condensation was simultaneously inhibited in this initial biorefinery stage. Subsequently, the enzymatic saccharification of the pretreatment residue was significantly enhanced due to the reduced cellulase adsorption of lignin. Lignin structural characterization demonstrated that VA could effectively incorporate to the lignin structure, resulting in the lignin exhibiting high preservation of β -O-4′ linkages (39.38/100 Ar), low molecular weight (2410 Da), abundant phenolic hydroxyl groups (3.70 mmol/g), and less degree of condensation (0.33). Importantly, the resulting less-condensed lignin (LCL) could effectively undergo melt-blending with poly(butylene adipate- co -terephthalate) (PBAT) owing to its low glass transition temperature, thereby facilitating the establishment of enhanced intermolecular interactions (π–π interaction and hydrogen bond) between LCL and PBAT. These interactions enhanced the plasticization of PBAT on LCL, and as a result, the prepared P/LCL 30 composite film possessed excellent tensile properties without requiring lignin modification or additional additives. The study presents a straightforward and efficient biorefinery conversion strategy for lignocellulosic biomass, wherein hemicellulose was degraded into XOS, cellulose was enzymatically hydrolyzed to glucose, and the remaining lignin with lower condensation was utilized as a filler of PBAT composite film.
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ENERGY & FUELS
·
2024
·
IF 5.2
Bo Wang,Wen Cai,Huai Liu,Rui Zhang,Wenlong Jia,Junhua Zhang,Lincai Peng
Catalytic hydrogenation of carbohydrates over the Raney Ni catalyst is an important industrial process to produce valuable polyols. However, this process generally encounters challenges such as harsh reaction conditions, low catalytic activity, and catalyst deactivation. In this contribution, metallic nickel-rich nickel borides (NiBO-nKH2PO4) were successfully constructed via a simple chemical reduction method with potassium dihydrogen phosphate (KH2PO4) as the buffering agent and sodium borohydride (NaBH4) as the reducing reagent. Especially, the obtained NiBO-10KH2PO4 afforded quantitative sorbitol yield from the catalytic hydrogenation of glucose under mild reaction conditions within 1 h (80 °C and 1 MPa H2). Compared with the reported literature for the production of sorbitol from glucose, the developed NiBO-10KH2PO4 catalyst circumvents the use of a high hydrogen pressure and reaction temperature while delivering excellent catalytic performance and stability. Moreover, the experimental analysis demonstrated that the obtained nickel borides, with the assistance of KH2PO4, possessed abundant metallic nickel species, which favored the adsorption and activation of hydrogen molecules and thus largely promoted the hydrogenation of glucose. This work provides new insights into the rational design of practical nickel-based catalysts for the efficient catalytic hydrogenation of carbohydrates.
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INTERNATIONAL JOURNAL OF BIOLOGICAL MACROMOLECULES
·
2024
·
IF 7.7
Yuanqi Duan,Zhengyu Hu,Long Jin,Tieqiang Zong,Xiaohui Zhang,Yanan Liu,Pengcheng Yang,Jinfeng Sun,Wei Zhou,Gao Li
The degradation of Belamcanda chinensis (L.) DC. polysaccharides was carried out by five concentrations of trifluoroacetic acid (TFA) (1–5 mol/L), and their physicochemical properties, degradation kinetics and anticomplementary activity were investigated. The findings revealed a notable reduction in the molecular weight of BCP, from an initial value of 2.622 × 10 5 g/mol to a final value of 6.255 × 10 4 g/mol, and the water solubility index increased from 90.66 ± 0.42 % to 97.78 ± 0.43 %. The degraded polysaccharides of B. chinensis exhibited a comparable monosaccharide composition comprising Man, GalA, Glc, Gal, and Ara. As the concentration of TFA increased, the degradation rate constant increased from 1.468 × 10 −3 to 5.943 × 10 −3 , and the process followed the first-order degradation kinetic model (R 2 > 0.97) and the random fracture model (R 2 > 0.96). Furthermore, the five degraded polysaccharides still exhibit good thermal stability. In vitro experiments showed that DBCP-3 exhibited more potent anticomplementary activity than the original polysaccharides and positive drugs, which was strongly correlated with its Mw ( r = 0.6–0.8), inhibiting complement activation by blocking C2 and C4. These results indicated that TFA degradation has a positive effect on polysaccharides, of which DBCP-3 is expected to treat diseases involving hyperactivation of the complement system.
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高影响因子
INDUSTRIAL CROPS AND PRODUCTS
·
2025
·
IF 6.2
Guoqiang Han,Wanqi Wang,Jialong Lv,Baibing Zhao,Zijian Zhang,Yan Zeng,Yongzhuang Liu,Haipeng Yu,Xiaoxue Song
Fractionation of softwood lignocellulose poses challenges due to its relatively high lignin content and complex interlinkages. In this study, an acidified deep eutectic solvent (choline chloride/ethylene glycol with 1 wt% AlCl₃) was employed to effectively fractionate Chinese fir chips into cellulose-rich and lignin fractions, achieving good to excellent yields. The cellulose-rich fraction was directly valorized into lignin-containing cellulose nanofibers (LCNFs) with a high yield. The prepared LCNFs were utilized as functional nanofillers in waterborne polyurethane wood coatings (WWC), resulting in an increase in tensile strength from 15.2 ± 0.35 MPa to 18.5 ± 0.43 MPa with the optimal addition of 0.5 wt% LCNFs. Furthermore, the addition of LCNFs significantly improved the coating properties, including hardness and wear resistance. The remaining lignin in the LCNFs imparted excellent optical properties to the WWC film, imparting a warm amber hue and improved texture convexity after coating. Analysis of the lignin fractions revealed a high total content of β-O-4 linkages. Following a catalytic hydrogen transfer depolymerization process, 9.8 wt% of propylguaiacol was obtained with near-quantitative selectivity. This study highlights the effectiveness of acidified deep eutectic solvents in the refinery of softwood lignocellulose, providing a novel pathway for the production of value-added bio-based nanomaterials and chemicals.
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RENEWABLE ENERGY
·
2024
·
IF 9
Qiuli Xia,Lin Zhang,Peng Zhan,Zhaohui Tong,Yan Qing,Jiaying He,Zhiping Wu,Hui Wang,Lishu Shao,Na Liu
The cost-effective and low-carbon fractionation of lignocellulosic biomass will enhance the economic viability of bio-refining. To achieve efficient reed fractionation under favorable conditions, microwave-assisted choline chloride and p -toluene sulfonic acid (M-ChCl/ p -TsOH) pretreatment was employed. The fractionation of reed components demonstrated higher effectiveness with M-ChCl/ p -TsOH pretreatment compared to conventional DES pretreatment. Under M-ChCl/ p -TsOH conditions, a high hemicellulose removal rate (∼90%) and delignification rate (∼65%) were achieved while achieving a saccharification rate of 88% during cellulase hydrolysis. Microwave assistance not only significantly reduced reaction time to less than 60 s but also enhanced the pretreatment efficacy. Furthermore, the obtained lignin products consisted of low-polymerization lignin (M W < 800 g/mol) and acid-soluble lignin, providing a solid foundation for subsequent high-value utilization of lignin. This study provides a promising strategy for the low carbon fractionation of reed using DES, aiming to simultaneously achieve efficient cellulose hydrolysis and obtain tractable lignin.
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Food Production Processing and Nutrition
·
2024
·Vol. 6
·期 1
·1-17
·
IF 4
Sun Yujiao,Li Baobao,Xue Yuanye,Wang Jiankang,Miao Bingbing,Liu Yang,Li Yanjun,Cao Yungang,Chang Dawei
Stipes are the major waste from the processing of Lentinus edodes . To make full use of L. edodes stipes (LES), different fractions of LES polysaccharides (LESPs) were first obtained by water extraction and gradient ethanol precipitation. Afterwards, the LES residues were treated with an optimal combination of Aspergillus niger and Saccharomyces cerevisiae for the preparation of soluble/insoluble dietary fibers (LESS/LESI) using the response surface methodology and the Box-Behnken design. Subsequently, the in vitro immunomodulatory activity of LESPs and LESS, as well as the adsorption capacities of LESI were evaluated. The results showed that LESPs were neutral polysaccharides, mainly containing glucose. The optimal parameters for modifying the residues of LES were the followings: 4% ( w / w ) A. niger , 8% ( w / w ) S. cerevisiae , 31 ℃, 3 d, and a solid–liquid ratio of 1:12.5 in a yield of 14.73%/82.45% of LESS/LESI, respectively. The in vitro immunomodulatory activity assays revealed that LESPs and LESS had potent immunostimulatory activity to increase phagocytosis, acid phosphatase activity and nitric oxide production of RAW264.7 murine cell macrophages. The evaluation of adsorption capacities revealed that LESI owned stronger water holding capacity, oil holding capacity and water swelling capacity. This research could provide an effective way to fully utilize discarded L. edodes stipes with high added-value. Graphical Abstract An effective strategy was built to fully utilize discarded L. edodes stipes by preparing polysaccharides (LESPs-20, LESPs-50 and LESPs-80), soluble and insoluble dietary fibers (LESS/LESI). Their products showed immunomodulatory activity and adsorption capacities.
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LWT-FOOD SCIENCE AND TECHNOLOGY
·
2024
·
IF 6
Dongsheng Hu,Gaoji Yang,Xiaogang Liu,Yuyue Qin,Fujie Zhang,Zhiqing Sun,Xinle Wang
Drying is a crucial process in the production of tea. The effects of sun drying (SD), hot air drying (HAD), microwave drying (MD), vacuum drying (VD) and freeze drying (FD) on the color, taste, bioactive, and aroma of coffee pulp tea (CPT) were evaluated systematically. The results showed that FD could better retain the original color, total phenolics (4.75 mg/mL), trigonelline (0.31 mg/mL), chlorogenic acid (0.067 mg/mL), protocatechuic acid (0.81 mg/mL), and total soluble sugars (24.33 mg/mL) of CPT, but its drying time was longer (32.56 h), and the taste and flavor were the worst. HAD and SD displayed the highest loss rates of most bioactive components, exceeding 40%. In contrast, MD can better improve the quality of CPT, giving it a better color, superior retention of bioactive components, and the most attractive taste and aroma, and decrease the drying time by 66.1% and 96.5%, respectively. Overall, MD is a promising production method for obtaining high-value-added CPT products.
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