PROCESS BIOCHEMISTRY
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2024
·
IF 3.7
Fanjin Meng,Qian Zheng,Yuqiu Wu,Lishi Yan,Zhi Chen,Xin Ju,Liangzhi Li
The enzymes used for D-allose production generally failed to meet the requirements of industrial production of D-allose in terms of enzyme activity and catalytic efficiency. In this study, 13 single mutants of CfRpiB (Ribose-5-phosphate isomerase from Curtobacterium flaccumfaciens ) were constructed by combining site-specific mutations and ring-open residue mutations with rational design. It was found that the enzyme activities of all 13 single mutants were positively changed relative to the wild type. Among them, the single mutant with the highest increase in enzyme activity was C72M, which increased its enzyme activity by 30 %. On this basis, three iterative mutant strains were constructed, among which C72M/S105H increased the yield of D-allose by 2.62 times. Especially, enzymatic property measurements showed that its enzyme activity increased by 50 %. Molecular docking of the C72M/S105H mutant with D-allulose revealed that the increased enzymatic activity of the mutant C72M/S105H could be attributed to the smaller substrate binding site and tighter binding to the substrate. This study further reveals the relationship between mutation sites and enzymatic properties, demonstrating the feasibility of improving the efficiency of preparing rare sugars through enzyme molecular modification.
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TALANTA
·
2024
·
IF 5.6
Jiayu Zhang,Haiyan Qi,Tonghui Yi,Tao Jing,Ming Zhao,Jun Li,Maoxia Ran,Xiaochen Zhu,Chao Luo
A highly sensitive ultra-small ratiometric fluorescence nanosphere probe was successfully manufactured to detect Sunset Yellow (SY). The probe, CMCS@N, S-CDs/Rh6G, was formed through the encapsulation of N, S-CDs and Rh6G within carboxymethyl chitosan (CMCS) through in situ cross-linking. Remarkably, our nanosphere probe had an average grain diameter of 6.80 nm and exhibited excellent dispersibility without the need for additional solvents. The probe exhibited a strong linear relationship with SY concentration in the range of 0.26–100 μM, with a low detection limit of 0.078 μM. Furthermore, SY demonstrated strong fluorescence quenching capability on our nanosphere probe, with the fluorescence quenching mechanism involving a combined effects of inner filter effect (IFE) and static quenching. Notably, our nanosphere probe retained the bacteriostatic properties of CMCS , with a substantial bacteriostasis rate of 77.58 %, introducing novel potential applications.
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Processes
·
2024
·Vol. 12
·期 4
·687
·
IF 2.8
Yannan Xiang,Siyi Tian,Xinyu Luo,Chenggang Cai,Yaowen Du,Hailong Yang,Haiyan Gao
The content of differentially abundant metabolites in the fermentation broth of grapefruit peels fermented byCordyceps militarisat different fermentation times was analyzed via LC–MS/MS. Small molecule metabolites and differential metabolic pathways were analyzed via multivariate analysis and Kyoto Encyclopedia of Genes and Genomes (KEGG) enrichment. A total of 423 metabolites were identified at 0, 2, 6, and 10 days after fermentation. Among them, 169 metabolites showed differential abundance, with significant differences observed between the fermentation liquids of every two experimental groups, and the metabolite composition in the fermentation liquid changed over the fermentation time. In summary, the upregulation and downregulation of metabolites in cancer metabolic pathways collectively promote the remodeling of cancer cell metabolism, facilitating increased glycolysis, alterations in TCA cycle flux, and enhanced biosynthesis of the macromolecules required for rapid proliferation and survival. This study provides new perspectives on the development of high-value-added agricultural and forestry byproducts and the development and research of functional foods.
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DIAMOND AND RELATED MATERIALS
·
2025
·
IF 5.1
Jinyan Du,Shiji Liu,Jinrui Hou,Xinyue Wu,Haojie Si,Xingya Guo,Shujuan Zhuo
Due to the abuse of traditional antibiotics, the increasing prevalence of antibiotic resistance poses a significant challenge to the finding of novel antimicrobial materials. Curcumin, a natural polyphenolic compound, exhibits broad-spectrum antibacterial, anti-inflammatory, and antioxidant properties. However, its therapeutic utility is limited by its bioavailability. To improve the biological activity of curcumin, the highly water-soluble carbon dots ( E -CDs) were synthesized by a one-step hydrothermal method using curcumin and ethylenediaminetetraacetic acid disodium salt (Na 2 EDTA) as precursors. It was shown that the surfaces of E -CDs still remain the structural and functional groups of their precursors. The as-obtained E -CDs exhibited a higher antimicrobial efficacy against Staphylococcus aureus ( S. aureus ) and Escherichia coli ( E. coli ) with the minimum inhibitory concentration (MIC) of only 40 and 150 ppm, respectively. Strikingly, the E -CDs did not induce significant bacterial resistance while exhibiting excellent biocompatibility. In a mouse model of bacterial-induced wound infection, the wound closure had approached 60 % within just four days, highlighting their significant potential for accelerating wound healing. Our experimental results convincingly demonstrated that the E -CDs are a promising candidate for clinical applications in treating infected wounds and combating drug-resistant bacterial infections.
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Surfaces and Interfaces
·
2024
·
IF 5.7
Cijun Shuai,Gao Pan,Qi Zhong,Shuping Peng
Bacterial infections considerably limit the success of scaffold transplantation. CuO 2 may generate hydroxyl radicals through a self-cascade catalytic process, which is effective for sterilization. However, reducing CuO 2 -derived Cu + ions to Cu° can lead to a decline in catalytic activity. To overcome these limitations, we anchored CuO 2 onto MoS 2 to establish stable Cu-S-Mo interfacial sites. The CuO 2 /MoS 2 nanozyme was fabricated using a low-temperature co-precipitation technique and introduced into poly- l -lactic acid scaffolds using selective laser sintering. The Cu-S-Mo interfacial sites showed a triad of functions that synergistically enhanced catalytic performance. These sites stabilized the Cu atoms, augmented catalytic activity by facilitating efficient electron transfer through the Mo 4+ /Mo 6+ redox couple, and improved the conduction of heat derived from the photothermal response of MoS 2 to the Cu catalyst. The synergistic action of chemodynamic and photothermal therapy, mediated by the Cu-S-Mo sites, resulted in remarkable antibacterial efficacy, with 92.3 % and 93.5 % inhibition rates against Staphylococcus aureus and Escherichia coli , respectively. Experimental evidence and simulations demonstrated that Cu-S-Mo structure stabilized the Cu atoms and improved electron transfer via interatomic charge transfer. These results highlight the potential of the CuO 2 @MoS 2 /PLLA scaffold in eliminating bacterial infections.
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IRANIAN POLYMER JOURNAL
·
2024
·
IF 2.4
Li Manli,Zhu Shengbin,Li Xiaohong,Jin Enqi
Graft polymerization-quaternization double modification has become a new effective method for preparing antibacterial chitosan (CS) in recent years. To improve the properties of double modified CS, the effects of molecular structures of amino-containing acrylate (ACA) monomer grafted onto CS (CS- g -PACA) and a quaternary ammonium reagent used for the quaternization of CS- g -PACA have been investigated in the study. A series of ACA monomers, i.e., dimethylaminoethyl methacrylate (DMAEMA), diethylaminoethyl methacrylate (DEAEMA) and 2-( tert -butyl amino)ethyl methacrylate (TBAEMA), were firstly grafted onto the molecular chain of CS. Then, CS- g -PACA was quaternized by various quaternary ammonium reagents, i.e., three kinds of chloralkane include 1-chlorobutane, 1-chlorooctane and 1-chlorododecane. The modified CS was blended with PVA to prepare nanofiber membranes by electrospinning. The major functional properties of the nanofiber membranes, e.g., fiber morphology, thermal stability, tensile property, water stability, and inhibition of E.coli and S.aureus as representatives of gram negative and positive bacterial, were evaluated systematically. The results showed that when ACA and chloralkane were TBAEMA and 1-chlorobutane, respectively, the graft-quaternization double modified CS/PVA blend nanofiber membrane possessed a more uniform and smooth fiber structure, higher thermal stability, tensile strength, water stability, and better inhibition function to the bacteria. For instance, the diameter of the fiber could reach ~ 180 nm and tensile strength of the fiber membrane could touch the value of 20.1 MPa as well. The inhibition rate of E.coli and S.aureus was as high as 95.9% and 99.6%, respectively. Double modified CS nanofibers have shown great potential to be used as biocompatible and environment-friendly medical and hygiene textiles. Graphical abstract
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Horticulturae
·
2024
·Vol. 10
·期 6
·587
·
IF 3.1
Jiangmei Hu,Junhuo Cai,Xinrui Hu,Lijun Wang,Qiangqiang Cheng,Xiuhua Tao
Turpinia argutais an excellent medicinal plant mainly used for the treatment of pharyngitis, tonsillitis, and tonsillar abscesses. However, an efficient regeneration protocol using tissue cultures forT. argutadoes not exist. Its main medicinal constituents are flavonoids, particularly ligustroflavone and rhoifolin. Here, we aimed to establish a tissue culture system forT. argutafor the first time using annual stem segments with axillary buds harvested from the field of the Jiangxi Academy of Forestry as explants by dynamically determining the accumulation of effective functional components in the tissue culture plantlets. Orthogonal tests were conducted to compare the effects of different explant disinfection times, media, and exogenous hormone ratios on the induction of the axillary bud growth, successional proliferation, and rooting ofT. argutastem segments. The best explant disinfection effect was achieved by disinfecting theT. argutaexplant with 75% ethanol for 50 s, followed by 0.1% mercuric chloride (HgCl2) for 6 min, and the optimal media for successional proliferation and rooting were Murashige and Skoog (MS) + 0.2 mg/L of 6-benzyladenine (6-BA), + 0.03 mg/L of naphthaleneacetic acid (NAA), and ½ MS + 2.5 mg/L of indole-3-butyric acid + 0.5 mg/L of NAA, respectively. The detection of ligustroflavone and rhoifolin in tissue culture plantlets 0, 3, and 5 months after transplanting showed a significant increasing trend and eventually exceeded the content requirements of the 2020 Edition ofChinese Pharmacopoeia forT. arguta. Our findings provide, for the first time, an effective tissue culture system forT. arguta, thereby providing important information to support the germplasm preservation, innovation, and application ofT. argutain the future.
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JOURNAL OF HAZARDOUS MATERIALS
·
2024
·
IF 12.2
Ying Liu,Jingfeng Gao,Qian Wang,Hao Chen,Yi Zhang,Xiaoyu Fu
Whether it’s necessary to extra chemical synthesis steps to modify nZVI in peroxymonosulfate (PMS) activation process are worth to further investigation. The 56 mg/L nZVI/153.65 mg/L PMS and 56 mg/L sulfidated nZVI (S-nZVI) (S/Fe molar ratio = 1:5)/153.65 mg/L PMS) processes could effectively attain 97.7% (with k obs of 3.7817 min −1 ) and 97.0% (with k obs of 3.4966 min −1 ) of the degradation of 20 mg/L sulfadiazine (SDZ) in 1 min, respectively. The nZVI/PMS system could quickly achieve 85.5% degradation of 20 mg/L SDZ in 1 min and effectively inactivate 99.99% of coexisting Pseudomonas . HLS-6 (5.81-log) in 30 min. Electron paramagnetic resonance tests and radical quenching experiments determined SO 4 •− , HO•, 1 O 2 and O 2 •− were responsible for SDZ degradation. The nZVI/PMS system could still achieve the satisfactory degradation efficiency of SDZ under the influence of humic acid (exceeded 96.1%), common anions (exceeded 67.3%), synthetic wastewater effluent (exceeded 90.7%) and real wastewater effluent (exceeded 78.7%). The high degradation efficiency of tetracycline (exceeded 98.9%) and five common disinfectants (exceeded 96.3%) confirmed the applicability of the two systems for pollutants removal. It’s no necessary to extra chemical synthesis steps to modify nZVI for PMS activation to remove both chemical and biological pollutants.
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POLYMER BULLETIN
·
2024
·
IF 3.1
Yang Se,Zhang Chunyan,Yong Liu,Niu Mei,Cheng Wei,Zhang Li,Xue Baoxia
Antibacterial dressings play an important role in wound management, especially in chronic wounds. Due to the excellent water retention, antibacterial hydrogel has become an ideal wound dressing. In this paper, the effective antibacterial dressing was constructed with the poly N-isopropylacrylamide (PNIPAM) hydrogel and graphene oxide loading nanosilver particles (GO-Ag) by the in situ chemical crosslinking method. The molecular structure, viscosity and mechanical properties, antibacterial agent content, antibacterial performance, temperature-sensitive antibacterial relationship and biocompatibility of PNIPAM-based GO-Ag composite hydrogel (PNIPAM/GO-Ag) were systematically characterized by the scanning electron microscopy (SEM), infrared spectroscopy (FT-IR), plate counting and other methods. The results showed that the introduction of GO-Ag increased the pore structure of PNIPAM/GO-Ag hydrogel and provided more active sites for the release of silver nanoparticles, wherein the content of nanosilver in PNIPAM/GO-Ag hydrogel was only 0.01%, meeting the requirements of application as antibacterial dressings. Moreover, when the hydrogel was subjected to heat contraction, silver nanoparticles could release more quickly and efficiently. The antibacterial rates of the PNIPAM/GO-Ag hydrogel against E. coli and S. aureus were 99.8 and 99% at 37 °C, respectively, higher than those at 25 °C (92, 95%). Compared with the pure PNIPAM hydrogel (1423.0%), the swelling rate of PNIPAM/GO-Ag hydrogel could reach 2024.6%, certifying its ability to absorb exudate of wound site. When the deformation was less than 80%, the hydrogel could maintain excellent elastic deformation recovery ability, and the viscosity of hydrogel at human body temperature was 38 Pa·s. These prove that it had a good fit with the skin, could dissipate more energy and restore shape. The hemolysis rate of PNIPAM/GO-Ag hydrogel was less than 5%, showing good bio-safety. These all demonstrated the PNIPAM/GO-Ag hydrogel can be used as a potential application for the skin wound dressing. Graphical abstract
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BIOMACROMOLECULES
·
2025
·
IF 5.4
Yujia Lu,Guanyi Li,Yanwen Zhang,Yuxuan Ge,Bin Hao,Yu Yin,Yaxue Zhao,Yin Wang
Antimicrobial peptides (AMPs) are a class of peptides consisting of cationic amino acid residues and a hydrophobic segment, which have been used as an alternative to antibiotics in treating multidrug-resistant bacteria. However, the relationship among the molecular design, assembled structures, and resultant efficacy remains elusive. Herein, we report a class of constitutionally isomeric AMPs assembled into filaments with similar dimensions. Spectroscopic characterizations demonstrated that subtle changes in the position of amino acids led to dramatic variations in molecular packing and surface charges, which were verified by molecular dynamics simulations. In vitro antibacterial assays showed that all AMPs exerted antibacterial activity against Gram-positive methicillin-resistant Staphylococcus aureus (MRSA), but the efficacy was dependent on the molecular design. Given the good biocompatibility to eukaryotic cells, these AMPs could be potentially used as antibacterial agents. We believe that this finding provides an avenue to tune the bioactivity of AMPs by rational molecular design.
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Advanced Composites and Hybrid Materials
·
2025
·Vol. 8
·期 1
·1-12
·
IF 21.8
Liu Min,Ma Weihua,Zhang Mai,Huang Shuyi,Lin Shishi,Liao Jianjun,Zhang Ping,Zhang Linlin
Marine environmentally friendly antifouling materials are emerging as a viable and promising alternative to the conventional toxic antifouling agents. Within this field, the exploration of natural antifouling substances has become a significant research focus, representing an auspicious avenue for innovation in eco-friendly technologies. In this study, we delve into the development of eco-friendly antifouling coatings through a novel chemical modification process. By incorporating natural antifouling agents onto an acrylic acid substrate through a grafting process, we have successfully synthesized three distinct varieties of natural antifouling coatings: isobornyl acrylate polymer (IBAP), acrylate indole polymer (AIP), and indole isobornyl co-modified acrylate polymer (IAA-IBOMA). Through meticulous surface characterization, structural analysis, and a comprehensive suite of antifouling performance tests, our findings indicate that these coatings exhibit superior antifouling properties. Notably, the IAA-IBOMA coating demonstrated exceptional anti-adhesion effects. The specific inhibition rates against E. coli , S. aureus , and Pseudoalteromonas aeruginosa were impressive, achieving 93.5%, 92.8%, and 95.7%, respectively. Moreover, the anti-mussel selective adhesion inhibition rate was found to be 93.3%. Furthermore, environmental toxicity assessments have validated the eco-friendly and stable nature of the IAA-IBOMA coating. These results underscore the potential of these natural product-based coatings as sustainable solutions for the marine industry. This work offers valuable insights and holds significant implications for guiding the future development of environmentally friendly antifouling coatings, steering the industry towards a more sustainable and eco-conscious direction.
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Bioactive Materials
·
2024
·
IF 18
Yinghao Li,Dongling Xiao,Weixi Yan,Meilin Jiang,Ju Tan,Zhongliang Qin,Jingting Zhou,Yue Sun,Mingcan Yang,Guanyuan Yang,Yawei Gu,Yong Liu,Chuhong Zhu
Timely and stable sealing of uncontrolled high-pressure hemorrhage in emergency situations outside surgical units remains a major clinical challenge, contributing to the high mortality rate associated with trauma. The currently widely used hemostatic bioadhesives are ineffective for hemorrhage from major arteries and the heart due to the absence of biologically compatible flexible structures capable of simultaneously ensuring conformal tough adhesion and biomechanical support. Here, inspired by the principle of chromatin assembly, we present a tissue-conformable tough matrix for robust sealing of severe bleeding. This hierarchical matrix is fabricated through a phase separation process, which involves the in-situ formation of nanoporous aggregates within a microporous double-network (DN) matrix. The dispersed aggregates disrupt the rigid physical crosslinking of the original DN matrix and function as a dissipative component, enabling the aggregate-based DN (aggDN) matrix to efficiently dissipate energy during stress and achieve improved conformal attachment to soft tissues. Subsequently, pre-activated bridging polymers facilitate rapid interfacial bonding between the matrix and tissue surfaces. They synergistically withstand considerable hydraulic pressure of approximately 700 mmHg and demonstrate exceptional tissue adhesion and sealing in rat cardiac and canine aortic hemorrhages, outperforming the commercially available bioadhesives. Our findings present a promising biomimetic strategy for engineering biomechanically compatible and tough adhesive hydrogels, facilitating prompt and effective treatment of hemorrhagic wounds.
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