EUROPEAN JOURNAL OF PHARMACEUTICAL SCIENCES
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2018
·
IF 3.466
Wen-Bin Zhao,Chi-Xiao Qiu,Ying Shen,Wen-Hui Liu,Jie Zhou,Ying-Chun Xu,Zhan Zhou,Shu-Qing Chen
Antibody-drug conjugates (ADCs) consist of cytotoxic agents covalently conjugated to monoclonal antibodies that substantially improve antitumour activity and reduce systemic toxicity. With the growing number of ADCs in clinical applications, more accurate bioanalysis data are urgently needed to facilitate the development and rational use of ADCs. Herein, we used antigen-positive cells as antigen carriers and ofatumumab (OFA-HL) and ofatumumab-based ADC (OFA-HL-MMAE) as examples to establish a new ligand-binding assay (LBA) method based on flow cytometry. We proved that the new method met the required analytical performance criteria and the lower limit of quantitation (LOQ) was 0.2 μg/mL. In addition, the LOQ of the quantitative OFA-HL flow cytometry method was reduced to 0.025 μg/mL by choosing an optimized fluorescent antibody, which indicated that the LOQ of the new method can be improved. What's more, the new method showed good stability and specificity when we used it to determine the concentrations of OFA-HL and OFA-HL-MMAE in mouse serum. During the bioanalysis of ADCs, various factors should be considered. Therefore, choosing optimal methods for ADC bioanalysis is necessary. This new method using in situ antigens not only extends the scope of application of the conventional LBA methods by avoiding the need for soluble antigens, but also improves the authenticity of ADC bioanalysis as a supplementary approach, which is valuable for developing accurate ADC assays.
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SPECTROSCOPY LETTERS
·
2015
·
IF 0.852
Bo Zhang,Yanbin Su,Lei Chen,Zhuofu Wu,Fengrong Zhang,Yanwen Su,Gang Zhao,Zhengqiang Li
The characteristic changes in UV-visible and Raman spectra of lithocholic acid on E-2-Butenal for antiglioma are the maximum absorption peak of E-2-Butenal from 302 to 319 nm, the decreases of Raman signal of 1688 cm−1 and 1641 cm−1 in E-2-Butenal by lithocholic acid with the phenomenon of 1688 cm−1 splitting, and the disappearance of the band at 932 cm−1, which clearly displays the change of wagging in methyl. In the presence of mitochondrial protein deriving from glioma, the above mentioned effects of lithocholic acid on E-2-Butenal are more significant by Raman spectra detections. The molecular targets of lithocholic acid on E-2-Butenal are carbonyl group, conjugated double bonds, and methyl, respectively. The study concludes that the interaction between lithocholic acid and E-2-Butenal decreases the carbonyl group bond strength in E-2-Butenal and then changes the structure and function of E-2-Butenal. The internal cause of the interaction between lithocholic acid and E-2-Butenal leading to the change of the carbonyl group bond strength is the electronic delocalization near the carbonyl group bond in E-2-Butenal. Since E-2-Butenal derives from glioma in the process of lipid peroxidation of mitochondria, our investigation significantly contributes to understanding the mechanism of action of lithocholic acid as a potential antiglioma drug.
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ECS Journal of Solid State Science and Technology
·
2024
·Vol. 13
·期 3
·037005
·
IF 1.8
Ning Lu,Boyu Guo,Zhikang Rao,Juntao Chen,Ying Xu
Electrochemical impedance sensors are widely used for bacterial detection and interpreted by the equivalent circuit model (ECM) to evaluate the concentration changes on the electrode interface. However, this method is mainly based on prior models and focuses on frequency domain analysis which cannot precisely describe unknown dynamic processes and a fractional surface coverage of electrode. Distribution of Relaxation Times (DRT) method, as an alternative to interpret impedance spectra can be applied to solve the problem. In this research, two electrode schemes with different modification steps were designed to collect impedance data. An Ensemble Tree-SHAP model was then adopted to quantitatively analyze the impedance data processed by ECM-DRT method in time-frequency domain and applied for the detection of Escherichia coli (E.coli). The results showed that using a dataset integrated with time-frequency domain features significantly improved the accuracy of bacterial concentration prediction. As a result, the RMSE of untrained concentration with LightGBM model was 6.4\times 1{0}^{-4}\,log CFU ml−1, with the sensor detection limit about 2.69 log CFU ml−1. The research provided a tool combining time-frequency analysis for accurate prediction of bacterial concentration, which is crucial for evaluation of the antibacterial effect of long-term antibiotics on E.coli in future.
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ENVIRONMENTAL TOXICOLOGY
·
2024
·
IF 4.4
Ling-Zi Xia,Li-Lan Liu,Jun-Zhe Yue,Zhen-Yu Lu,Ru-Ya Deng,Xi He,Can-Can Li,Burong Hu,Hai-Tao Gao
Objective Phthalates (PEs) could cause reproductive harm to males. A mixture of three widely used PEs (MPEs) was used to investigate the ameliorative effects of zinc (Zn) and vitamin E (VE) against male reproductive toxicity. Methods Fifty male SD rats were randomly divided into five groups ( n = 10). Rats in MPEs group were orally treated with 160 mg/kg/d MPEs, while rats in MPEs combined Zn and/or VE groups were treated with 160 mg/kg/d MPEs plus 25 mg/kg/d Zn and/or 25 mg/kg/d VE. After intervention for 70 days, it's was measured of male reproductive organs' weight, histopathological observation of sperms and testes, serum hormones, PIWI proteins and steroidogenic proteins. Results Compared with control, anogenital distance, testes weight, epididymides weight, and sex hormones were significantly decreased, while the sperm malformation rate was markedly increased in MPEs group ( p < .05); the testicular tissues were injured in MPEs group with disordered and decreased spermatids, and arrested spermatogenesis. PIWIL1, PIWIL2, StAR, CYP11A1 and CYP19A1 were down-regulated in MPEs group ( p < .05). However, the alterations of these parameters were restored in MPEs combined Zn and/or VE groups ( p < .05). Conclusion Zn and/or VE improved steroid hormone metabolism, and inhibited MPEs' male reproductive toxicity.
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APPLIED THERMAL ENGINEERING
·
2025
·
IF 6.9
Yanjun Sun,Haiqi Zheng,Bohao Yin,Xiaopo Wang,Maogang He
With global energy consumption surging and environmental concerns escalating, absorption refrigeration systems are receiving increasing attention. This study investigates the absorption behavior and thermodynamic performance of absorption refrigeration systems using trans -1-chloro-3,3,3-trifluoro-1-propene (R-1233zd(E)) paired with phosphonium-based ionic liquids (ILs), trihexyltetradecylphosphonium bis(trifluoromethylsulfonyl) amide ([P 66614 ][Tf 2 N]) and trihexyltetradecylphosphonium bis(2,4,4-trimethylpentyl) phosphinate ([P 66614 ][TMPP]). Experimental solubility measurements of R-1233zd(E) in the ILs were conducted using an isochoric saturation method across a temperature range of 293.15–343.15 K and pressures up to 0.61 MPa. The Non-Random Two-Liquid (NRTL) thermodynamic model was employed to correlate solubility data, yielding excellent agreement with deviations of less than 3.84 %. Results revealed that [P 66614 ][TMPP] exhibits higher solubility due to its branched alkyl chains, increased free volume, and favorable van der Waals interactions. Thermodynamic analyses were performed for single-effect (SARS), double-effect (DARS), and compression-assisted cycles (LC-SARS, single-effect cycle with a compressor added in the low-pressure side; HC-SARS, single-effect cycle with a compressor added in the high-pressure side; LC-DARS, double-effect cycle with a compressor added in the low-pressure side). It demonstrated that [P 66614 ][TMPP] exhibits a higher coefficient of performance (COP) compared to [P 66614 ][Tf 2 N]. Compression assistance reduced the minimum generation temperature and improved COP by leveraging electrical energy. Among all configurations, LC-DARS with a compression ratio of 1.9 demonstrated the optimal balance between COP (0.82) and thermal energy utilization efficiency. These findings highlight the potential of R-1233zd(E)/[P 66614 ][TMPP] as a sustainable working pair for absorption systems, particularly in low-grade heat recovery applications.
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CHEMICAL ENGINEERING JOURNAL
·
2025
·
IF 13.2
Hui Ma,Qiuhong Wang,Siyan Li,Huidi Huang,Juan Zhang,Yuanfeng Wang,Wen Wu,Xiaohui Yu,Jiajia Shen
Achieving large-scale, ion-selective sensing remains a critical challenge in the development of wearable health-monitoring systems with clinical relevance. Here, we present a bio-inspired sea-island electrostatic heterostructure that enhances ion selectivity by engineering a spatially patterned charge interface, in which graphene (Gr) serves as a negatively charged sea and polylysine modified Fe 3 O 4 (PLL@Fe 3 O 4 ) nanoparticles act as positively charged islands. This heterostructure generates local electrostatic gradients that preferentially facilitate K + over Na + transport based on differences in hydration shell deformability. To enable scalable fabrication, we developed a shear-thinning PLL@Fe 3 O 4 /Gr ink suitable for screen printing, allowing the creation of flexible e-textile sensor arrays. The Na + /K + selectivity coefficient improved significantly from ~0.95 (Gr) to ~1.45 (PLL@Fe 3 O 4 /Gr), representing an enhancement of approximately 53 %. The printed sensors exhibited excellent stability during continuous sweat monitoring and successfully generated early warning signals under simulated hypokalemia conditions. This work provides a scalable and effective strategy for constructing high-performance, ion-selective textile biosensors, bridging fundamental ion transport principles with real-world applications in wearable healthcare platforms.
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INTERNATIONAL JOURNAL OF BIOLOGICAL MACROMOLECULES
·
2019
·
IF 4.784
Jianying Huang,Yuhong Liu,Lijun Yang,Fengyan Zhou
A sulfonated chitosan (SCS) was prepared via nucleophilic substitution and characterized by Fourier transform infrared spectroscopy, 1 H and 13 C NMR spectra, gel permeation chromatography, elemental analysis, and thermo gravimetric analysis. The inhibition activities of bacterial adhesion and biofilm formation against E. coli and S. aureus of SCS were assessed in comparison with those of unmodified chitosan hydrochloride (WCS) which was commercially available. The metabolic activity and secretion of exopolysaccharide in biofilms of E. coli and S. aureus were significantly decreased after the treatment of SCS. Scanning electron microscopy and confocal laser scanning microscopy also demonstrated that SCS and WCS at 1MIC concentrations could obviously inhibit the formation of biofilm. Upon the experimental data obtained, it can be concluded that the alkylsulfonation of chitosan could significantly improve efficacy in killing biofilm-embedded bacteria, and the inhibition activities against biofilm formation of E. coli and S. aureus . The exploitation of SCS in this study is helpful to extend the understanding to an alternative to antibiotics and chemical preservatives in food and medicine fields.
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ACS Applied Engineering Materials
·
2023
·
IF 0
Yuchao Chen,Li Wang,Yufei Shu,Qi Han,Beizhao Chen,Mengxia Wang,Xun Liu,Danyal Rehman,Bei Liu,Zhongying Wang,John Lienhard
The economic value of recovering gold from electronic waste (e-waste) has generated significant interest, but selective capture of gold from complex acidic electronic leaching solutions remains challenging. Here, we synthesized poly-m-phenylenediamine (PmPD) nanoparticles with a positively charged surface and amino functional groups, resulting in an adsorption capacity of 2063 mg/g for Au(III) in acidic solutions, superior to most traditional adsorbents. Electrostatic adsorption and reduction were identified as the adsorption mechanism for Au(III) by zeta potential, X-ray diffraction, transmission electron microscope, Fourier transform infrared, and X-ray photoelectron spectroscopy analyses. To enable adsorbent recycling, PmPD nanoparticles were assembled into adsorptive membranes and used for gold recovery from e-wastewater via a continuous-flow membrane separation process. The PmPD membrane achieved a dynamic gold recovery capacity of approximately 530 mg/g and could be effectively regenerated after washing with a mixture of thiourea and HCl. We demonstrated the practical application of the adsorptive membranes by recovering about 100% of gold from the leaching solution of waste printed circuit boards of computers. Finally, the recovered gold nanoparticles on the PmPD membrane were used to catalyze the degradation of p-nitrophenol, showcasing the catalytic property of gold. The Au@PmPD membrane loaded with 4 mg gold exhibited a high catalytic reduction performance with an apparent rate constant of 0.59 min–1, one of the highest catalytic degradation rate constants of p-nitrophenol reported to date. Our study presents an effective and economical approach for recovering gold from e-waste, providing a prototype of resource recovery and reuse.
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JOURNAL OF CHROMATOGRAPHY B-ANALYTICAL TECHNOLOGIES IN THE BIOMEDICAL AND LIFE SCIENCES
·
2019
·
IF 2.813
Neng Xiong,Ru Yu,Tao Chen,Ya-Ping Xue,Zhi-Qiang Liu,Yu-Guo Zheng
Methionine is an essential sulfur-containing amino acid for organisms. The separation and purification are important for the production of l -methionine from fermentation broth. In this work, the adsorption properties for l -methionine separation of ten macroporous resins were firstly evaluated. Macroporous cation resin D72 showed the best adsorption capacity (52.37 mg/g) and desorption rate (99.12%). The adsorption kinetics of l -methionine on D72 resin followed the pseudo second-order model and adsorption isotherm fitted well to the Sips model, the adsorption process mainly followed a physisorption mechanism. D72 resin packed columns were then used in the batch separation of fermentation broth, the operation parameters were optimized during the dynamic adsorption and desorption experiments. Under the optimized conditions: fermentation broth adjusted to pH = 2, loading flow rate at 2 BV/h, loading volume 55 mL, 1 mol/L NH 3 ·H 2 O as eluent , elution flow rate at 2 BV/h, column height-diameter ratio at 14:1, excellent recovery and purity of l -methionine (82.37% and 85.69%, respectively) could be achieved.
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RSC Advances
·
2015
·Vol. 5
·期 37
·29185-29192
·
IF 3.84
He Li,Jun-Mian Tian,Hao-Yu Tang,Shi-Yin Pan,An-Ling Zhang,Jin-Ming Gao
Fifteen aromatic polyketide metabolites, including four new chromones, chaetosemins B–E (2–5), with compound 4 bearing a new skeleton, and two new natural products, chaetosemin A (1) and (+)-(S)-chaetoquadrin J (14), together with nine known ones (6–13, 15), were isolated from the organic extract of a solid fermented culture of an Ascomycete filamentous fungus, Chaetomium seminudum. The structures of 1–5 were determined by spectroscopic analysis, and the absolute configuration of chaetosemin A (1) was confirmed by X-ray crystallography, whereas the stereochemistry of 1–4 and 14 was assigned by comparison of their specific optical rotations with reported data for structurally related compounds. All compounds were isolated for the first time from Chaetomium seminudum. Compounds 1 and 2 consisted of L-cysteine and D-cysteine units, respectively. Most of them were evaluated for in vitro antifungal activities against some phytopathogenic fungi. Among them, chaetosemin B (2) was the most active against Magnaporthe oryzae and Gibberella saubinettii with MICs of 6.25 and 12.5 μM, respectively. In addition, chaetosemin C (3) showed antioxidant activity with 50.7% DPPH free radical scavenging activity at 50 μM, and chaetoquadrin J (14) displayed weak inhibition of soluble epoxide hydrolase (sEH).
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Journal of Cleaner Production
·
2024
·
IF 9.7
Weichun Gao,Shengnan Chen,Cong Geng,Yinyan Guan,Yingjun Gao,Jiyan Liang
Given the limitations of unequal H 2 O 2 and O 3 distribution in the E-peroxone (EP) process, a novel dual-wavelength UV enhanced E-peroxone process (VUV/UVC/EP) with high performance for removing emerging contaminants was established. In this paper, carbamazepine (CBZ) removal rates by different oxidation processes were investigated, calculating the kinetic effects of the VUV/UVC/EP processes in various operating parameters, and the degradation mechanisms and pathways of CBZ were comprehensively discussed. Compared with conventional EP, ultraviolet (UVC, wavelength 254 nm), UVC/EP, and dual-wavelength ultraviolet (VUV/UVC, wavelength 185 and 254 nm) processes, the VUV/UVC/EP process exhibits an excellent ability for degradation of carbamazepine (CBZ), the synergy coefficient between VUV/UVC and EP process is 1.44 (>1). In particular, the VUV/UVC/EP process exhibits exceptional degradation performance even at 50 mg L −1 CBZ. It also demonstrates high adaptability to varying pH, humic acid concentration, and different inorganic anions (SO 4 2− , Cl − , NO 3 − , PO 4 3− and CO 3 2− ). In addition, ·OH, 1 O 2 , and·O 2 − were identified to exist in the VUV/UVC/EP process. The dominant reactive species ·OH content was increased by decomposing the excess dissolved O 3 to accelerate CBZ degradation. CBZ was broken down into a series of intermediates, NO 2 − , NO 3 − , and other inorganic compounds through ring reduction and oxidation reactions under the attack of reactive oxygen species (ROS) generated in the VUV/UVC/EP process. It also can remove more than 50% of TOC in 30 min and decrease biological toxicity. An electrical energy-per-order (EEO) value of 3.37 kW h m −3 indicated that the VUV/UVC/EP process is economically feasible. This comprehensive examination provides valuable insights into the CBZ removal in reclaimed water, contributing to the advancement of environmental water resources.
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BIOSENSORS & BIOELECTRONICS
·
2025
·
IF 10.5
An Tang,Jiachen Wang,Jia-Li Xu,Jun-Tao Liu,Tianpeng Xu,Qiang Zhang,Yu Tian
The integration of visualization and electrical feedback in biomimetic electronic skin (E-skin) for real-time motion tracking has attracted significant attention in wearable electronics. To facilitate human-readable interactive feedback and improve the overall performance of E-skin, particularly regarding mechanical compliance, sensitivity, and stability, we propose a novel high-performance photonic-ionic skin (PI-skin) based on mechanochromic photonic ionogel. The PI-skin, prepared by incorporating aligned magnetic colloids into an ionogel matrix, integrates interactive structural coloration and electrical responses to external stimuli, achieving dual-mode precise motion recognition. Due to the enhanced hydrogen bonding and electrostatic interactions within the ionogel matrix, the PI-skin exhibits excellent mechanical properties (tensile strain >1000%), remarkable adhesive strength (peeling force >7 kPa), high sensitivity (gauge factor = 1.42 at straining 0%–300% and 2.71 at straining 300%–500%), rapid response time (120 ms), and exceptional mechanical compliance (>500 continuous cycles). More importantly, such PI-skin demonstrates ultrafast and highly accurate optical/electrical dual-signal motion detection, thereby facilitating posture correction during sitting and encoding of vocal cord vibrations during speech. We anticipate that this versatile PI-skin will possess substantial potential in the field of wearable electronics, and may inspire the design of next-generation E-skin for human-computer interaction and personalized healthcare applications.
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