Nano Research
·
2023
·Vol. 16
·期 12
·13366-13374
·
IF 9.9
Lin Chaoxin,Kim Kumchol,Wang Zuochao,Yan Zhuang,Tang Zhiyong,Liu Yaling
Integration and synergy of the unique functions of different components have been developed into one of the most convenient and effective ways to construct the composite advanced materials with collective properties and improved performances. In this work, the mace-like tetrathiafulvalene-tetracyanoquinodimethane (TTF-TCNQ)/HKUST-1 composite structures with single single-crystalline TTF-TCNQ submicrorods covered by ordered HKUST-1 nanosheet arrays were successfully constructed by an efficient TTF-TCNQ seed-mediated growth approach. Impressively, thanks to the synergetic and complementary effects between TTF-TCNQ and HKUST-1, the sensors based on such mace-like TTF-TCNQ/HKUST-1 composite structures not only displayed an experimental detection limit of 10 part per billion (ppb) for NO 2 detection, but also exhibited outstanding selectivity even if the concentration of the interfering gases was 10 times that of NO 2 . Meanwhile, good reproducibility and rapid response were also achieved. This work opens the avenue for creation of novel high-performance sensing materials for application in gas sensing.
研究领域:
材料科学
·
化学传感
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纳米技术
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气体传感器
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DOI
高影响因子
JOURNAL OF CHEMICAL AND ENGINEERING DATA
·
2022
·
IF 3.119
Zhen Jiao,Mengnan Zhang,Lixia Yang,Yuxuan Wang,Jiajie Fu
To evaluate the feasibility of extraction of 1-methylimidazole from its low-concentration aqueous solution by ionic liquids, the liquid–liquid equilibrium of the ternary system of water + 1-methylimidazole + ionic liquid ([Hmim][PF6], [Omim][PF6], or [Omim][BF4]) was determined at 303.15 K and atmospheric pressure. The distribution coefficient and selectivity were calculated in accordance with the experimental results. The effects of the anion and cation of ionic liquids on the separation ability were discussed. The results indicated that the extraction capacity increased with increasing cationic carbon chain length, and the change of anions significantly affected the distribution coefficient and selectivity. As expected, the three ionic liquids were feasible as extractants for separating 1-methylimidazole from water. It also indicated that [Omim][PF6] was a more suitable extractant than [Hmim][PF6] and [Omim][BF4] for the extraction. The highest distribution coefficient and selectivity of [Omim][PF6] could reach 1.71 and 111.92, respectively, indicating [Omim][PF6] as a good choice for extracting 1-methylimidazole from water. In addition, the non-random two-liquid model was employed to successfully correlate the experimental data with a root-mean-square deviation of less than 0.0188. Moreover, a GUI-MATLAB tool was used to verify the consistency of the correlated parameters.
研究领域:
化学工程
·
材料科学
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DOI
高影响因子
JOURNAL OF CHEMICAL AND ENGINEERING DATA
·
2022
·
IF 3.119
Yingmin Yu,Xiaocheng Zhang,Lingxiao Yi,Min Li,Fangyu Zhang,Jiajun Wei
Liquid–liquid equilibrium (LLE) data of ternary systems {2-ethyl-1-hexanol + ethylene glycol + water/n-heptane} were measured at 298.2, 308.2, and 318.2 K under 101.3 kPa. The distribution coefficient (D) and selectivity coefficient (S) of the systems were calculated to analyze the extraction ability of the two extractants that separate 2-ethyl-1-hexanol and ethylene glycol. Meanwhile, the NRTL and UNIQUAC activity coefficient models were used to correlate the experimental data and the different model parameters were obtained by data regression. The root-mean-square deviation (RMSD) between the calculated and experimental values was less than 0.96%, indicating that both models have a reasonable correlation with the experimental data of the ternary system and can accurately predict the experimental data. GUI-MATLAB has validated the binary interaction parameters.
研究领域:
化学工程
·
热力学
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DOI
高影响因子
SEPARATION AND PURIFICATION TECHNOLOGY
·
2019
·
IF 5.107
Rui Wang,Xingang Li,Jian Na,Yan Wu,Runnan Zhao,Yutao Yan,Hong Li,Xin Gao
Reversible reaction-assisted technology for ethanediol (EG) and 1, 2-butanediol(1,2-BDO) azotropic mixture separation was proved effective. However, the missing acetalization kinetic parameters hinder the process design and industrialization. This paper addresses the competitive kinetics of EG and 1,2-BDO mixture towards propyl aldehyde. The reaction mechanism was illustrated, and the impacts of feed composition, temperature and solution inhomogeneity were explored. The pseudo-homogeneous model was derived for the system, and the kinetic parameters were obtained. Results demonstrated that the aldehyde and 2-ethyl-1,3-dioxione (2ED) inputs were crucial manipulation variables for reversible reaction-assisted separating (RRAS) process design. The RRAS process was proposed, based on the competitive kinetics, to evaluate the techno-economic performance of RRAS. Two promising processes, which differed in the conversion of EG, were proposed to implement the separation. The economic evaluation revealed that the flowsheet with EG and 1,2-BDO both being converted to acetals and EG then being recovered through 2ED hydrolyzation, has more potential for industrial application.
研究领域:
化学工程
·
工业催化
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DOI
高影响因子
DIGESTIVE DISEASES AND SCIENCES
·
2022
·Vol. 67
·期 10
·4780-4796
·
IF 3.487
Duan Houyu,Ding Xiang,Luo Hesheng
Background and Aims KISS-1 is an established tumor suppressor that inhibits metastases in various malignancies. However, little is known regarding its role in esophageal squamous cell carcinoma (ESCC). The aim of the present study was to identify the possible mechanisms of KISS-1 in ESCC metastasis. Methods The expression levels of KISS-1 mRNA and protein in ESCC samples and cell lines were analyzed by qRT-PCR, IHC, and western blotting. Bisulfite sequencing PCR (BSP) and methylation-specific PCR (MSP) were used to analyze the methylation pattern of KISS-1 promoter in ESCC cells with or without 5-Aza-dC treatment. The role of KISS-1 in the progression and metastasis of ESCC was analyzed through in vitro functional assays. Results KISS-1 mRNA and protein were markedly downregulated in ESCC tissues and cell lines compared to the respective controls. Hypermethylation of KISS-1 promoter correlated to its lower expression levels in ESCC, and KISS-1 demethylation inhibited tumor progression. Ectopic KISS-1 overexpression inhibited tumor cell metastasis in vitro. In addition, KISS-1 overexpression downregulated the matrix metalloproteinase 2 and 9 (MMP2 and 9) and inhibited epithelial–mesenchymal transition (EMT). Finally, KISS-1 downregulated phosphorylated extracellular regulated protein kinase 1/2 (ERK1/2) and phosphorylated p38 mitogen-activated protein kinase (MAPK) without affecting their total expression levels in the ESCC cells. MAPK/ERK and p38 MAPK agonists reversed the suppressive effects of KISS-1. Conclusions The hypermethylation of KISS-1 promoter partly contributed to its downregulation in ESCC. KISS-1 inhibits the metastasis of ESCC cells by targeting the MMP2/9/ERK/p38 MAPK axis.
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产品关联
DOI
PubMed
高影响因子
Discover Oncology
·
2024
·Vol. 15
·期 1
·1-12
·
IF 2.8
Liu Yueli,Li Fan,Wang Qiongyu,Zhang Yunfei,Tian Shuhong,Li Biao
Anlotinib is effective in treatment of many kinds of malignant cancer, but its antineoplastic effects on esophageal cancer remains unclear. This study aims to investigate its impact on esophageal cancer and the underlying mechanisms. Anlotiniband 5-fluorouracil + cisplatin (5-FU + DDP) was administered separately to human esophageal cancer TE- 1 cells tumor xenograft mouse models every 3 days. Tumor size and body weight were measured before each treatment and at the end of the experiment. In vitro studies were conducted using TE- 1 cells to examine the effects of Anlotinib. Cell viability, migration, proliferation, apoptosis, cell cycle, their regulatory proteins and the transcriptomic changes were analyzed. Anlotinib reduced tumor size, tumor weight, and the ratio of tumor weight to body weight in vivo. It decreased the viability of TE- 1 cells, with a 50% growth-inhibitory concentration of 9.454 μM for 24 h, induced apoptosis, and arrested TE- 1 cell cycle in the S phase. It inhibited migration and proliferation while negatively regulating the PI3K/Akt signaling pathway. Enhanced expressions of P21, Bax, and lowered expressions of cyclin A1, cyclin B1, CDK1, PI3K, Akt, p-Akt, and Bcl-2 were observed after Anlotinib treatment. Anlotinib exhibits antineoplastic activity against human esophageal cancer TE- 1 cells by negatively regulating the PI3K/Akt signaling pathway, consequently altering the expressions of proteins related to proliferation, apoptosis, and the cell cycle.
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产品关联
DOI
PubMed
高影响因子
ANALYTICAL AND BIOANALYTICAL CHEMISTRY
·
2020
·Vol. 412
·期 11
·2413-2421
·
IF 3.637
Wang Xia,Zhang Yawen,Zhao Liying,Wang Dazhong,Yang Huaixia,Kong Jinming
An ultrasensitive fluorescence biosensor for detecting cytokeratin fragment antigen 21-1 (CYFRA 21-1) DNA of non-small cell lung carcinoma (NSCLC) is designed using polysaccharide and activator regenerated by electron transfer atom transfer radical polymerization (ARGET ATRP) signal amplification strategy. Thiolated peptide nucleic acid (PNA) is fixed on magnetic nanoparticles (MNPs) by a cross-linking agent and hybridized with CYFRA 21-1 DNA. Hyaluronic acid (HA) is linked to PNA/tDNA heteroduplexes in the form of carboxy-Zr 4+ -phosphate. Subsequently, multiple 2-bromo-2-methylpropionic acid (BMP) molecules are linked with HA to initiate ARGET ATRP reaction. Finally, a large number of fluorescein o-acrylate (FA) monomers are polymerized on the macro-initiators, and the fluorescence signal is significantly amplified. Under optimal conditions, this biosensor shows a significant linear correlation between the fluorescence intensity and logarithm of CYFRA 21-1 DNA concentration (0.1 fM to 0.1 nM), and the limit of detection is as low as 78 aM. Furthermore, the sensor has a good ability to detect CYFRA 21-1 DNA in serum samples and to recognize mismatched bases. It suggests that the strategy has broad application in early diagnosis by virtue of its high sensitivity and selectivity.
A novel and highly sensitive fluorescence biosensor for quantitatively detecting CYFRA 21-1 DNA via dual signal amplification of hyaluronic acid and ARGET ATRP reaction was developed. This proposed method has a low detection limit, wide detection range, high selectivity, and strong anti-interference.
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产品关联
DOI
PubMed
高影响因子
Cancer Nanotechnology
·
2024
·Vol. 15
·期 1
·1-16
·
IF 4.5
Jiang Qiping,Yao Fengjiao,An Yacong,Lai Xialian,Li Xundou,Yu Zhen,Yang Xian-Da
Background PD-1/PD-L1 blockade plays a crucial role in cancer immunotherapy. Exploration of new technologies to further enhance the efficacy of PD-1/PD-L1 blockade is therefore of potential medical importance. Nanotherapeutics can accumulate in tumor tissues due to enhanced permeability and retention (EPR) effects. In this study, a novel nanotherapeutic for cancer immunotherapy was implemented with albumin nanoparticles functionalized by both PD-1 and PD-L1 aptamers.Results Albumin nanoparticles (NP) were functionalized with either PD-1 aptamers (PD1-NP), PD-L1 aptamers (PDL1-NP), or both types of aptamers (PD1-NP-PDL1). Average sizes of PD1-NP, PDL1-NP, and PD1-NP-PDL1 were 141.8 nm, 141.8 nm, and 164.2 nm, respectively. PD1-NP had good affinity for activated T cells that expresses PD-1. Similarly, PDL1-NP could bind with MDA-MB-231 or CT26 tumor cells that express PD-L1. Moreover, the bispecific PD1-NP-PDL1 could bind with both the activated T cells and the PD-L1-expressing tumor cells, and tether the two type of cells together. Functionally, aptamer-modified nanoparticles exhibited stronger immune-stimulating effects vs. free aptamers. Specifically, PD1-NP or PDL1-NP induced stronger lymphocyte-mediated cytotoxicity against PD-L1-expressing tumor cells in vitro vs. free PD-1 or PD-L1 aptamers. Animal studies also showed that PD1-NP or PDL1-NP significantly improved antitumor efficacy against CT26 colon cancer in vivo vs. free PD-1 or PD-L1 aptamers. Importantly, the bispecific PD1-NP-PDL1 further boosted the in vivo antitumor efficacy compared with PD1-NP or PDL1-NP, without raising systemic toxicity.Conclusion The results suggest that the bispecific PD1-NP-PDL1 is a promising nanotherapeutic to improve the efficacy of PD-1/PD-L1 blockade, and may have application potential in colon cancer treatment.
研究领域:
肿瘤学
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纳米医学
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免疫学
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DOI
高影响因子
RSC Advances
·
2016
·Vol. 6
·期 30
·25427-25434
·
IF 3.289
Zulin Hua,Qin Qin,Xue Bai,Xin Huang,Qi Zhang
A highly efficient enzyme-based screen printed electrode (SPE) was developed based on the covalent immobilization of tyrosinase (Tyr) on 1-formylpyrene (1-FP) functionalized reduced graphene oxide (rGO). Here, the bifunctional molecule 1-FP was assembled onto rGO sheets. Subsequently, a Tyr molecule was immobilized on the 1-FP forming a biocompatible nanocomposite, which was further coated onto the working electrode surface of the SPE. The performance of as-prepared biosensor was investigated by the detection of phenol in the presence of molecular oxygen. Wide linear range, low detection limit and high sensitivity were obtained with this biosensor due to the good conductivity of rGO as well as the high bioactivity of Tyr well retained by the 1-FP/rGO platform. Finally, the proposed biosensor was successfully employed for the detection of phenol in real water samples with satisfactory results. These findings suggest that this novel biosensor could offer great potential for rapid, cost-effective and on-field analysis of phenolic compounds.
研究领域:
分析化学
·
环境科学
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DOI
高影响因子
npj Regenerative Medicine
·
2023
·Vol. 8
·期 1
·1-17
·
IF 7.2
Zhao Chuanrong,Yang Qianru,Tang Runze,Li Wang,Wang Jin,Yang Fangfang,Zhao Jianan,Zhu Juanjuan,Pang Wei,Li Ning,Zhang Xu,Tian Xiao Yu,Yao Weijuan,Zhou Jing
Healing of the cutaneous wound requires macrophage recruitment at the sites of injury, where chemotactic migration of macrophages toward the wound is regulated by local inflammation. Recent studies suggest a positive contribution of DNA methyltransferase 1 (Dnmt1) to macrophage pro-informatory responses; however, its role in regulating macrophage motility remains unknown. In this study, myeloid-specific depletion of Dnmt1 in mice promoted cutaneous wound healing and de-suppressed the lipopolysaccharides (LPS)-inhibited macrophage motility. Dnmt1 inhibition in macrophages eliminated the LPS-stimulated changes in cellular mechanical properties in terms of elasticity and viscoelasticity. LPS increased the cellular accumulation of cholesterol in a Dnmt1-depedent manner; cholesterol content determined cellular stiffness and motility. Lipidomic analysis indicated that Dnmt1 inhibition altered the cellular lipid homeostasis, probably through down-regulating the expression of cluster of differentiation 36 CD36 (facilitating lipid influx) and up-regulating the expression of ATP-binding cassette transporter ABCA1 (mediating lipid efflux) and sterol O-acyltransferase 1 SOAT1 (also named ACAT1, catalyzing the esterification of cholesterol). Our study revealed a Dnmt1-dependent epigenetic mechanism in the control of macrophage mechanical properties and the related chemotactic motility, indicating Dnmt1 as both a marker of diseases and a potential target of therapeutic intervention for wound healing.
研究领域:
免疫学
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细胞生物学
·
分子生物学
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药理学
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DOI
PubMed
高影响因子
JOURNAL OF POLYMER RESEARCH
·
2018
·Vol. 25
·期 1
·1-9
·
IF 1.434
Li Hanyuan,Wang Wei,Chen Guofei,Liu Yinfeng,Fang Xingzhong
Novel highly soluble phenylethynyl-endcapped oligoimides derived from mixed thioetherdiphthalic anhydride isomers ( m -TDPA) and 5(6)-amino-1-(4-aminophenyl)-1,3,3-trimethylindane (DAPI), 4,4′-oxydianiline (4,4’-ODA) with 4-phenylethynylphthalic anhydride (PEPA) as a reactive endcapping agent were synthesized. The calculated molecular weights of all the oligoimides were 2500 g·mol −1 .The effect of the mole ratio of DAPI/4,4’-ODA on solubility and melt viscosity of oligoimides as well as the thermal and mechanical properties of cured polyimide films was investigated. Experimental results indicated that DAPI greatly improved the solubility (>30 wt.%) of the oligoimides in low boiling points solvents when the content of DAPI was more than 10 mol% per total diamine. All the oligoimides exhibited very good processability with minimum melt viscosity lower than 60 Pa·s at about 330 °C. Tough and brown films were obtained after thermally cured at 370 °C for 1 h and dynamic mechanical analysis (DMA) showed that glass transition temperatures of the cured films increased up to 340 °C with the increasing content of DAPI. However, the thermal stability and mechanical properties decreased with the increase of DAPI content. The temperature of 5% weight loss was higher than 470 °C in both air and N 2 atmosphere obtained by thermogravimetric analysis (TGA). And the tensile strengths of the cured films were about 60 MPa.
研究领域:
材料科学
·
化学工程
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DOI
高影响因子
Journal of Functional Foods
·
2024
·
IF 3.8
Shuwei Ke,Siyu Zhang,Dongsheng Liu,Tengjiao Zhao,Xiayan Lou,Sunying Cheng,Tingheng Zhu,Hanbing Li
Ectopic olfactory receptors, such as OR1A1, are involved in the regulation of lipid metabolism homeostasis. In this study, we investigated the effects of three monoterpenes, (+)-limonene, (−)-carvone and thymol, upon hepatic lipid deposition. Oral administration of (+)-limonene, (−)-carvone and thymol for 8 weeks in high-fat-diet (HFD)-fed mice significantly lowered the levels of fasting blood glucose, improved glucose intolerance, serum lipid parameters, adiposity, and hepatic steatosis. In LO2 cells, (+)-limonene, (−)-carvone and thymol increased the cyclic adenosine monophosphate (cAMP), induced protein kinase A (PKA) activity and phosphorylation of AMPK, downregulated the expression of SREBP-1 and FASN, and eventually led to a reduction in hepatic lipid deposition. Meanwhile, these beneficial effects in cultured LO2 cells were negated by OR1A1 knockdown and the adenylyl cyclase inhibitor SQ22536, respectively, indicating that they can ameliorate disorders of lipid metabolism through activating OR1A1 and subsequent AMPK/SREBP-1/FASN pathway, indicating OR1A1 as a potential target for counteracting hepatic steatosis.
研究领域:
药理学
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代谢学
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肝病学
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分子生物学
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DOI
高影响因子
危险品化学品经营许可证（带存储）