MATERIALS AND STRUCTURES
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2023
·Vol. 56
·期 1
·1-15
·
IF 3.8
Song Yufeng,Zhang Yi,Shen Siyi,Pan Chenyu,Yan Dongming,Wang Zhiguang,Wang Su,Ruan Shaoqin
This study aims to explore the effects of different Ca(OH) 2 contents on the mechanical performance and corrosion behaviors of steel in Calcium sulfoaluminate (CSA) cement, where compressive strength, corrosion potentials of reinforcements, corrosion current density, pH of pore solution as well as mortar resistivity of CSA/Ca(OH) 2 mortar were researched, and the corrosion behaviors were further interpreted by the clarification of hydration phases through microstructural analyses. The initial results highlighted the positive effects of using adequate Ca(OH) 2 (~ 6%) in CSA mortar in terms of strength, yet its excessive addition could delay the subsequent hydration reaction of CSA. Meanwhile, with an increased quantity of Ca(OH) 2 , the amount of some hydration phases, such as Al(OH) 3 reduced, resulting from their reaction with Ca(OH) 2 . Finally, the corrosion current density increased with the reduced mortar resistivity when the reinforcement in CSA/Ca(OH) 2 system was corroded, and their relationship was also provided in this study.
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Phytochemistry Letters
·
2014
·
IF 1.542
Guoyong Luo,Min Zhou,Yan Liu,Qi Ye,Jian Gu,Tianfang Huang,Guolin Zhang,Yinggang Luo
Two new 3-formyl-2-arylbenzofurans, iteafuranals A and B, were isolated from the 95% EtOH extract of the aerial parts of Itea ilicifolia . Their structures were elucidated as ( E )-2-(3,4-dihydroxyphenyl)-3-formyl-4-hydroxy-5-(prop-1-en-1-yl)benzofuran ( 1 , iteafuranal A) and ( E )-2-(3-hydroxy-4-methoxyphenyl)-3-formyl-4-hydroxy-5-(prop-1-en-1-yl)benzofuran ( 2 , iteafuranal B) by comprehensive analyses of spectroscopic data including 1- and 2-D NMR, UV, IR, and HRMS data. The structure of 1 was confirmed by X-ray crystallography analysis. Compound 1 showed DPPH free radical scavenging capacity with an IC 50 value of 0.34 mg/mL. Compounds 1 and 2 showed ABTS cation radical scavenging capacity with IC 50 values of 0.23 and 0.30 mg/mL, respectively.
研究领域:
植物化学
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药理学
·
天然产物研究
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Translational Research
·
2024
·
IF 6.4
Hao Chen,Qianchao Hu,Zhongshan Lu,Jie Zhao,Anxiong Liu,Zhongzhong Liu,Jun Luo,Qifa Ye,Zibiao Zhong
Renal ischemia-reperfusion injury (IRI) is a prevalent clinical syndrome, yet its underlying pathogenesis remains largely unknown. Aldehyde dehydrogenase 2 (ALDH2), an enzyme responsible for detoxifying lipid aldehydes, has been suggested to play a protective role against IRI. In our study, we observed that Aldh2 knock-out C57BL/6 mice experienced more severe renal functional impairment following IRI. This was characterized by elevated levels of creatinine and blood urea nitrogen, as well as increased apoptosis. Proteomic analysis further revealed that ALDH2 deficiency significantly disrupted lipid metabolism, resulting in higher levels of the proinflammatory protein CYP4A and its metabolic byproduct, 20-HETE. This metabolic disruption exacerbated renal inflammation and triggered endoplasmic reticulum stress. However, we found that administration of the CYP4A inhibitor, HET0016, could ameliorate these effects. Mechanistically, we discovered that after IRI, ALDH2 translocates to the nucleus and interacts with nuclear receptor corepressor 1 (NCOR1) to repress Cyp4a transcription. ALDH2 specifically interacts with the N-terminal domain of NCOR1, which is responsible for its interaction with its E3 ligase SIAH2. This interaction inhibits the proteasome degradation of NCOR1, ultimately stabilizing the NCOR1 transcriptional repression complex. In summary, our research uncovers the role of ALDH2 in mitigating renal IRI by inhibiting 20-HETE synthesis through the transcriptional repression of Cyp4a .
研究领域:
肾病学
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分子生物学
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药理学
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PubMed
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ANALYTICAL CHEMISTRY
·
2021
·
IF 6.986
Wei S. Zhang,Jianbin Pan,Feng Li,Min Zhu,Mengting Xu,Hongyan Zhu,Yanyan Yu,Gaoxing Su
The outbreak of the pandemic caused by the severe acute respiratory syndrome coronavirus-2 (SARS-CoV-2) calls for an urgent unmet need for developing a facial and cost-effective detection method. The requirement of well-trained personnel and sophisticated instrument of current primary mean (reverse transcription polymerase chain reaction, RT-PCR) may hinder the practical application worldwide. In this regard, a reverse transcription recombinase polymerase amplification (RT-RPA) coupled with CRISPR-Cas12a colorimetric assay is proposed for the SARS-CoV-2 detection. The methodology we have described herein utilizes DNA-modified gold nanoparticles (AuNPs) as a universal colorimetric readout and can specifically target ORF1ab and N regions of the SARS-CoV-2 genome. After the virus genome is amplified through RT-RPA, the resulting abundant dsDNA will bind and activate Cas12a. Under trans-cleavage degradation, the capped DNA substrate will be hydrolyzed gradually from AuNPs, demonstrating a change in the surface plasmon resonance (SPR), which can be facially monitored by UV–vis absorbance spectroscopy and naked eye observation. The high amplification efficiency from RT-RPA and Cas12a trans-cleavage process bring the sensitivity of our method to 1 copy of viral genome sequence per test. Notably, under the dual variations inspecting from the isothermal amplification and Cas12a activation process, the false positive events from other beta coronavirus members can be effectively avoided and thus significantly improve the specificity. Furthermore, the reliability of this colorimetric assay is validated by standard clinical samples from the hospital laboratory department. Through integration of the inherently high sensitivity and specificity from an RPA-coupled Cas12a system with the intrinsic simplicity of AuNP-based colorimetric assay, our method increases the practical testing availability of SARS-CoV-2.
研究领域:
分子生物学
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病毒学
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诊断学
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PubMed
高影响因子
SENSORS AND ACTUATORS B-CHEMICAL
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2020
·
IF 7.1
Wenxi Xia,Shuangzhe Zhang,Jiangli Fan,Yueqing Li,Xiaojun Peng
Combining imaging and treatment of cancer into one molecule has become an efficient strategy for studies on pathology and cancer therapeutics. Herein, a novel highly selective and sensitive green-emitting probe ( APLN ), which contained a fluorophore (Naphthalimide), a linker, and a COX-2 kinase inhibitor (aspirin) has been designed and evaluated. Cyclooxygenase-2 (COX-2), as one of the typical rate-limiting enzymes, has been utilized as imaging and therapeutic target since this enzyme is overexpressed in various cancer cell lines. Aspirin, a non-steroidal anti-inflammatory drug (NSAID), has been reported to reduce the incidence and risk of cancers by down-regulating the expression of COX-2 in cancer cells. The APLN probe has been proved to successfully label the COX-2 enzyme to identify tumors and significantly inhibit tumor growth in a xenotransplantation model of nude mice. Therefore, this enzyme-targeted fluorescence probe ( APLN) is a potential reagent for in situ imaging and fluorescent-visible cancer therapy.
研究领域:
医学
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生物化学
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肿瘤学
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高影响因子
ACS Omega
·
2025
·
IF 4.3
Tao Shen,Guodong Wu,Li Yin,Tianen Chen,JiYe Fan,Yuanhao Wang,Shifeng Wang
Developing efficient adsorbents for removal of organic pollutants and heavy metals is essential for environmental protection. In this study, a series of GO/UiO-66-(OH)2 composites were synthesized and characterized using XRD, FT-IR, and SEM, confirming enhanced thermal stability, reduced aggregation, and maintained crystallinity of UiO-66-(OH)2 due to GO incorporation. Batch adsorption experiments showed that GO increased the adsorption capacity, with GO-8/UiO-66-(OH)2 achieving 324.2 mg/g for diclofenac sodium (DCF) and 429.3 mg/g for Hg2+. DCF adsorption was primarily governed by physisorption, while Hg adsorption was chemisorptive, involving strong coordination with oxygen functional groups. Kinetic and isotherm studies revealed the suitability of the pseudo-second-order and Freundlich models, with thermodynamic analysis showing exothermic DCF adsorption and endothermic Hg2+ adsorption. The composites also exhibited high selectivity and stable regeneration, making them promising candidates for practical environmental remediation.
研究领域:
环境科学
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材料科学
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PubMed
高影响因子
CHEMICAL PHYSICS
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2020
·
IF 1.771
Yuejun Li,Tieping Cao,Zemin Mei,Xiaoping Li,Dawei Sun
Among tremendous efforts for enhanced photocatalysis, construction of heterojunction has been deemed as an effective means. In comparison with type II heterojunction, type I heterojunction receives less attention due to accumulation and increased recombination of charge carriers in one side of heterojunction. Herein, we take NaBi(MoO 4 ) 2 /Bi 2 MoO 6 as an example of type I heterojunction and explore means to improve the photocatalytic performance of NaBi(MoO 4 ) 2 /Bi 2 MoO 6 heterojunction. We employ TiO 2 electrospun nanofibers as substrates and separately grow NaBi(MoO 4 ) 2 and Bi 2 MoO 6 nanostructures on the skeleton of TiO 2 . The characterization results show that TiO 2 changes type I heterojunction into multiheterojunction integrating two type II heterojunctions. Photocatalytic tests demonstrate that as-constructed multi-heterojunction is of higher activity than that of single type I or II heterojunction, respectively, where photogenerated electrons are accumulated on the surface of TiO 2 and photogenerated holes are accumulated on NaBi(MoO 4 ) 2 and Bi 2 MoO 6 , respectively. This study highlights the potential application of TiO 2 electrospun nanofibers in the construction of multiheterojunction for enhanced visible light photocatalysis.
研究领域:
材料科学
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环境科学
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BIOTECHNOLOGY LETTERS
·
2021
·Vol. 43
·期 8
·1617-1624
·
IF 2.461
Wang Liuzhu,Jiang Shuiqin,Sun Yangyang,Yang Zeyu,Chen Zhi,Wang Hualei,Wei Dongzhi
Objectives Catalytic promiscuity, or the ability to catalyze a secondary reaction, provides new opportunities for industrial biocatalysis by expanding the range of biocatalytic reactions. Some nitrilases converting nitriles to amides, referred to as the secondary activity, show great potential for amides production. And our goal was exploiting the amide-forming potential of nitrilases. Results In this study, we characterized and altered the secondary activity of nitrilase from Acidovorax facilis 72 W (Nit72W) towards different substrates. We increased the secondary activity of Nit72W towards 2-cyanopyridine by 196-fold and created activity toward benzonitrile and p-nitrophenylacetonitrile by modifying the active pocket. Surprisingly, the best mutant, W188M, completely converted 250 mM 2-cyanopyridine to more than 98% 2-picolinamide in 12 h with a specific activity of 90 U/mg and showed potential for industrial applications. Conclusions Nit72W was modified to increase its secondary activity for the amides production, especially 2-picolinamide.
研究领域:
生物化学
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工业生物技术
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JOURNAL OF ORGANIC CHEMISTRY
·
2020
·
IF 4.335
Wubing Yao,Jiali Wang,Lili He,Dongdong Cao,Jianguo Yang
A ruthenium catalytic ortho-C–H diborylation of 2-arylpyridine derivatives, including challenging 2-phenoxypyridine functions, using a remarkably low catalyst loading and a low-cost and bench-stable boron source, has been developed. The novel strategy shows high activity with excellent selectivity and may offer a versatile and green alternative to currently employed high loadings of noble metals or extra additives for the selective borylations.
研究领域:
有机化学
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催化化学
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高影响因子
RSC Advances
·
2015
·Vol. 5
·期 57
·45693-45702
·
IF 3.84
Anming Li,Dekang Xu,Hao Lin,Shenghong Yang,Yuanzhi Shao,Yueli Zhang,Zhenqiang Chen
Well-crystallized and uniform NaGd(MoO4)2 microcrystals with morphologies of bipyramids, truncated bipyramids, quasi-cubes and tetragonal plates were selectively synthesized via a facile hydrothermal method without any additives under mild conditions. The effects of Na2MoO4/Gd(NO3)3 molar ratios and pH values of precursor solutions on the phase and morphology of the as-synthesized microcrystals were systematically investigated. The molar ratios and pH values played key roles in the selective synthesis of pure phase NaGd(MoO4)2 microcrystals with regular morphology. With increasing Na2MoO4/Gd(NO3)3 molar ratios in the appropriate pH range, the morphology of the products changed from bipyramids, truncated bipyramids, quasi-cubes to tetragonal plates, namely, the morphological truncation degree increased gradually. NaGd(MoO4)2 tetragonal microplates could be synthesized at the molar ratio of 9 : 1. A possible morphological formation mechanism of NaGd(MoO4)2 tetragonal microplates was proposed, i.e. nucleation-Ostwald ripening growth process. Color tunable photoluminescence properties of NaGd(MoO4)2:Eu3+,Tb3+ microcrystals were studied in detail. Among the different morphologies of NaGd(MoO4)2:5% Eu3+ microcrystals, tetragonal microplates had a broadened, red-shifted and enhanced charge transfer band in the excitation spectrum. What's more, the full-width at half-maximum for the charge transfer band of the tetragonal plates was highest (58 nm), which favored efficient excitation and absorption in the ultraviolet region. The introduction of a small amount of Tb3+ into NaGd(MoO4)2 microplates doped with Eu3+ would enhance the 5D0 → 7FJ transition of Eu3+ at 616 nm due to the energy transfer process of cross-relaxation from Tb3+ to Eu3+. Multicolor tunable luminescence from deep red, red, orange, yellow to green under 291 nm ultraviolet excitation and from red, reddish orange to pink under 380 nm near-ultraviolet excitation could be obtained in NaGd(MoO4)2 tetragonal microplates by simply adjusting the doping concentrations of Eu3+ and Tb3+, suggesting NaGd(MoO4)2:Eu3+,Tb3+ microcrystals might have practical application in optoelectronic devices, such as light emitting diodes and color display systems. This facile morphology-controlled hydrothermal synthesis strategy was simple, low-cost and environment-friendly, and might be extended to other inorganic materials.
研究领域:
材料科学
·
光学
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高影响因子
POWDER TECHNOLOGY
·
2021
·
IF 5.134
Mingyu Zheng,Tiefeng Peng,Yanhong Wang,Liqun Luo,Wei Shao,Jiaqi Gao,Qi Lu
Selective separation of pyrite and galena using 2-mercaptobenzimidazole (MBI) as a chelating collector, was investigated by flotation , scanning electron microscope (SEM), Fourier transform infrared (FT-IR), X-ray photoelectron spectroscopy (XPS) and density functional theory (DFT) calculations. Single-mineral flotation showed a significant flotability of pyrite at pH = 2–4 and galena at pH = 8–10 in the presence of MBI. The depressant effect of pyrite was observed to be more pronounced under alkaline conditions. Artificially mixed minerals flotation suggested the galena-pyrite could be separated under alkaline conditions, especially at pH = 10. SEM analysis indicates that the adsorption of MBI on galena surface is more intense than pyrite. However, the interaction between MBI and pyrite is stronger than that of MBI-galena. FT-IR analysis shows the iron and lead ions may chelate with -N = and -SH groups of MBI. Pyrite contains more iron hydroxide (or sulfate forms) in solution from XPS analysis, only half of the metallic ions on the surface function as active sites, thus reducing the amount of metal that can be chelated with MBI. DFT calculations revealed that MBI was often in the molecular form under acidic mediums and the ionic form under alkaline condition, while the ionic form of MBI was more active.
研究领域:
矿物学
·
化学工程
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高影响因子
JOURNAL OF AGRICULTURAL AND FOOD CHEMISTRY
·
2024
·
IF 5.7
Jie Zheng,Hongxiang Liu,Jiwen Zhang,Bo Yu
L-Azetidine-2-carboxylic acid (L-Aze), a natural nonproteinogenic amino acid found widely in plants, has recently been identified as an environmentally friendly agent for controlling powdery mildew with low toxicity. In this study, a biological route for L-Aze production via the methionine salvage pathway (Yang Cycle) was first in silico designed for Escherichia coli. Subsequently, systematic engineering strategies were employed to enhance the production efficiency, including the enhancement of the 5-phosphoribosyl 1-pyrophosphate (PRPP) supply, construction of the ATP-adenine cycle, and engineering of the strain’s resistance to L-Aze. The final strain produced L-Aze from glucose with a titer of 568.5 mg/L. The antifungal activity of the produced L-Aze in the fermentation broth was also confirmed for treating powdery mildew in cucurbits. This approach not only provides a sustainable and green route for pesticide production to control powdery mildew but also expands our understanding of the exogenous construction of the Yang Cycle in E. coli.
研究领域:
微生物学
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植物病理学
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环境科学
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PubMed
高影响因子
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