ECOTOXICOLOGY AND ENVIRONMENTAL SAFETY
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2024
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IF 6.2
Danni Jiang,Yang Yang,Xiaojuan Han,Qianni Li,Pengfei Li,Yang Xu,Lan Chao
Past studies have observed that BHPF induces multi-organ toxicity, however, whether it induces damage to male reproductive system and the specific mechanism remains unclear. In the present study, male mice were given 0, 2, 10 or 50 mg/kg/day of BHPF by gavage for 35 days to observe its effect on reproductive organ and sperm quality. The results indicated that BHPF decreased sperm count and sperm motility in a dose-dependent manner. Besides, our results demonstrated that BHPF triggered the proliferation inhibition and cell death of germ cells in vivo and in vitro. Also, BHPF reduced the expression of function markers for germ cells, Sertoli cells, and Leydig cells, indicating its damage to function of testis cells. Simultaneously, testicular microenvironment was found to be altered by BHPF, as presented with declined testosterone level and decreased expression of local microenvironment regulators. Overall, our findings indicated the detrimental effects of BHPF on male reproductive function in mice, suggesting testicular function and local microenvironment disturbance as mechanism underlying testicular damage.
研究领域:
毒理学
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生殖生物学
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内分泌学
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高影响因子
BIOLOGICAL TRACE ELEMENT RESEARCH
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2025
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IF 3.6
Liang Chen,Chen Mingyang,Mu Zhidan,Tian Xinyan,Zhao Wenzhen,Hu Yarong,Su Juan
The aim of this study was to explore the role of the ZnT9 protein in obesity-induced sperm maturation disorders in men. We generated a mouse model of obesity-induced weak spermatogenesis via a high-fat diet (HFD) for 10 weeks. In addition to the HFD, a 5-week intervention of salubrinal (SAL) (an inhibitor of endoplasmic reticulum stress) (1 mg/kg/day), ZnSO 4 (15 mg/kg/day), and their combination was started at week 6, after which sperm viability and epididymal tissue damage were assessed. To investigate the role of the ZnT9 protein in spermatogenesis, the expression levels of the ZnT9 protein, endoplasmic reticulum stress (ERS)–related protein, Wnt pathway protein, and apoptosis-related protein in epididymal tissue were measured. Compared with those in the normal (N) group, the mice in the HFD group presented decreased sperm motility, damaged epididymal tissue, epididymal tissue showed decreased expression of ZnT9, β-catenin, LEF protein and mRNA, and increased expression of total cholesterol (TC) and triglycerides (TG), GRP78, Caspase-3, BAX protein and mRNA, as well as increased apoptosis as shown by TUNEL staining. Compared with the HFD group, HFD + ZnSO 4 group, HFD + SAL group, and HFD + ZnSO 4 + SAL groups resulted in reduced epididymal damage, improved decreased total cholesterol (TC) and triglycerides (TG), sperm viability, increased expression of ZnT9, β-catenin, LEF protein and mRNA, and decreased expression of GRP78, Caspase-3, and BAX protein and mRNA, as well as decreased apoptosis as shown by TUNEL staining in epididymal tissues. According to this study, obesity leads to elevated ERS and affects ZnT9 protein synthesis. Inhibition of the Wnt pathway ultimately leads to cell death and damage in epididymal tissue and decreased sperm viability.
研究领域:
生殖生物学
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分子生物学
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营养学
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DOI
PubMed
高影响因子
Journal of Ocean University of China
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2026
·Vol. 25
·期 2
·586-598
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IF 1.6
Zeng Chu,Lyu Likang,Liu Mengqun,Yang Xiaodong,Zhao Hongkui,Wen Haishen,Li Jifang,Qi Xin
Caspase-9, the most intensively studied caspase, plays a crucial role in mitochondrial or intrinsic apoptosis of mammals as a protease. To explore the role of caspase-9 in fish, we cloned casp9 gene from rainbow trout ( Oncorhynchus mykiss ) and studied its characteristic and functions. Our in vivo experiments revealed that casp9 expression was significantly upregulated 24 h after bacterial challenge in the gills, accompanied by notable histopathological changes and apoptosis signals. Gene cloning identified that rainbow trout caspase-9 is a 455 amino acid protein encoded by a 1368 bp gene, characterized by a conserved CARD domain, as well as prominent large (P20) and small (P10) subunits. Functional assays of recombinant rainbow trout caspase-9 (rcaspase-9) showed dose-dependent proteolytic activity, with significantly higher activity at 2 mg/mL than at 0.2 mg/mL. Conversely, the CARD domain-deleted version exhibited minimal activity even at 2 mg/mL, demonstrating that the CARD domain is indispensable for homodimerization and self-activation. Additionally, RT-qPCR results indicated that casp9 was mainly expressed in gills and spleen. In vitro studies demonstrated significant upregulation of casp9, apaf-1 and cyt c , along with downregulation of bcl-2 in primary gill cells in response to LPS, peptidoglycan and poly I:C stimulation. Furthermore, rcaspase-9 induced upregulation of casp6 and casp7 in these cells. These findings underscore the importance of caspase-9 in immune defense and apoptosis triggered by pathogens in rainbow trout.
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高影响因子
LANGMUIR
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2013
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IF 4.187
Xinyu Wang,Yincong Zhang,Tianfu Li,Wende Tian,Qiang Zhang,Yiyun Cheng
Poly(amidoamine) (PAMAM) encapsulated platinum nanoparticles were synthesized and used as catalase mimics. Acetylated generation 9 (Ac-G9) PAMAM dendrimer with a molecular size around 10 nm was used as a template to synthesize platinum nanoparticles. The feeding molar ratio of Pt4+ and Ac-G9 is 2048, and the synthesized platinum nanoparticle (Ac-G9/Pt NP) has an average size of 3.3 nm. Ac-G9/Pt NP has a similar molecular size and globular shape with catalase (∼11 nm). The catalytic activity of Ac-G9/Pt NP on the decomposition of H2O2 is approaching that of catalase at 37 °C. Ac-G9/Pt NP shows differential response to the changes of pH and temperature compared with catalase, which can be explained by different catalytic mechanisms of Ac-G9/Pt NP and catalase. Ac-G9/Pt NP also shows horseradish peroxidase activity and is able to scavenge free radicals such as di(phenyl)-(2,4,6-trinitrophenyl)iminoazanium (DPPH). Furthermore, Ac-G9/Pt NP shows excellent biocompatibility on different cell lines and can down-regulate H2O2-induced intracellular reactive oxygen species (ROS) in these cells. These results suggest that dendrimers are promising mimics of proteins with different sizes and Ac-G9/Pt NP can be used as an alternative candidate of catalase to decrease oxidation stress in cells.
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PubMed
高影响因子
CHEMICAL ENGINEERING JOURNAL
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2024
·
IF 13.3
Wenhui Fang,Tingting Tang,Min Li,Shixian Xin,Ying Wu,Yutao Cao,Zhize Yu,Wei Cui,Zengxi Li,Hong Zhao
Designing catalysts at atomic level fine-tune the electronic structure of surface-active site for efficient water splitting, is a highly desirable approach but also poses significant challenges. This study focuses on the synthesis of a series of electronically tunable metal single-atom (M−SAs) catalysts: M 9 @S-Ni(OH) 2 (M=Pt, Mn, Fe or Cu). Experimental results reveal that introduction of trace S-heteroatoms and metal atoms into Ni(OH) 2 lattice can effectively modulate the electronic structure of M 9 @S-Ni(OH) 2 through modulation of p-d and d-d orbital hybridization, resulting in exceptional performance in water electrolysis. Among M 9 @S-Ni(OH) 2 , Pt 9 @S-Ni(OH) 2 and Fe 9 @S-Ni(OH) 2 exhibit excellent hydrogen evolution reaction (HER) and oxygen evolution reaction (OER) performance, respectively, the overpotential requiring only 7 mV and 269 mV, respectively, at current density of 10 mA cm −2 . (−) Pt 9 @S-Ni(OH) 2 ||Fe 9 @S-Ni(OH) 2 (+) presents a high current density of 531.07 mA cm −2 at cell-voltage of 1.80 V in overall-water splitting (OWS), which is 14.07 times higher than that of the commercial (−) Pt/C||IrO 2 (+) (37.86 mA cm −2 ) system. This work achieves the development of cost-effective and stable electrocatalysts, offering a solution for large-scale and sustainable water-hydrogen conversion.
研究领域:
催化
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材料科学
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电化学
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可再生能源
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DOI
高影响因子
Acta Pharmaceutica Sinica B
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2024
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IF 14.7
Yongnian Zeng,Chenyuan Wang,Jiapeng Lei,Xue Jiang,Kai Lei,Yinli Jin,Tianshu Hao,Wen Zhang,Jianying Huang,Wei Li
High glucose level, bacterial infection, and persistent inflammation within the microenvironment are key factors contributing to the delay of diabetic ulcers healing, while traditional therapeutic methods generally fail to address these issues simultaneously. Here, we present a spatiotemporally responsive cascade bilayer microneedle (MN) patch for accelerating diabetic wound healing via local glucose depletion and sustained nitric oxide (NO) release for long-term antibacterial and anti-inflammatory effects. The MN patch (G/AZ-MNs) possesses a degradable tip layer loading glucose oxidase (GOx), as well as a dissolvable base layer encapsulating L-arginine (Arg)-loaded nanoparticles (NPs). After wound administration, the base part rapidly dissolved, resulting in prompt separation of the MN tip within the wound tissue, which subsequently responded to the overexpressed matrix metalloproteinase-9 (MMP-9) in diabetic lesions, leading to the responsive release of GOx. The released enzyme catalyzed glucose into gluconic acid and hydrogen peroxide (H 2 O 2 ), which not only reduced glucose level within the diabetic wound, but also initiated the cascade reaction between H 2 O 2 with the Arg that was released from NPs, thereby achieving continuous production of NO for 7 days. Our findings demonstrate that a single administration of the MN patch could effectively heal non-infected or biofilm-infected diabetic wounds with the multifunctional properties.
研究领域:
生物医学工程
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药学
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材料科学
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DOI
PubMed
高影响因子
JOURNAL OF CHEMICAL RESEARCH
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2015
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IF 0.633
Fang-Ming Wang,Dan Bao,Bing-Xiang Hu,Ze-Yu Zhou,Deng-Deng Huang,Li-Zhuang Chen,Yuan-Yuan Dan
A series of novel 10-substituted 3,6-diphenyl-9-aryl-3,4,6,7,9,10-hexahydroacridine-1,8(2H,5H)-dione derivatives synthesised by condensation of different amines with xanthenes, was prepared from 5-phenylcyclohexane-1,3-dione and aromatic aldehydes by a Knoevenagel and Michael reaction and cyclisation catalysed by a trace of l-proline at room temperature. In addition, the molecular structure of a xanthene was determined by single-crystal X-ray diffraction analysis.
研究领域:
有机化学
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药物化学
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DOI
高影响因子
FULLERENES NANOTUBES AND CARBON NANOSTRUCTURES
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2026
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IF 2.2
Hai-Wen Tang,Jin-Jin Jiang,Ke-Ying Wu,Shi-Yong Mou,Lian-Xiang Chen,Yun-Xiang Chen,Su-Jun Wang,Yun-Qian Zhong,Liu-Si Yu
Sodium-ion batteries (SIBs) are promising alternatives to lithium-ion systems due to abundant resources and good low-temperature performance. However, iron-based sulfate cathodes suffer from poor electronic conductivity and unstable electrode–electrolyte interfaces. To address this, we combined DFT-guided interface design with a scalable synthesis method to build an optimized conductive network. Density functional theory (DFT) calculations identified the interfacial characteristics of weak sodium dodecyl sulfonate (SDS) adsorption and strong carbon adsorption, providing theoretical guidance for achieving uniform carbon dispersion and coating. Using sand-milling and spray-drying, we fabricated a Ketjenblack (KB)-coated core–shell cathode (NFS@KB-4%). The high-surface-area carbon enabled a continuous conductive network and self-assembly of primary particles into uniform spherical secondary particles (5–7.8 μm). This structure simultaneously enhanced electronic conductivity and structural stability. The NFS@KB-4% cathode delivered 84.03 mAh g−1 at 1 C, retained 91.1% capacity after 200 cycles, and exhibited a Na+ diffusion coefficient of 8.24 × 10−8 cm2 s−1—twice that of the control. A soft-pack full cell with hard-carbon anode maintained 97.2% capacity over 60 cycles, demonstrating practical viability. This work paves the way for large-scale use of high-rate, long-life iron-based sulfate cathodes in SIBs.
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高影响因子
ANALYTICAL AND BIOANALYTICAL CHEMISTRY
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2026
·
IF 4.1
Liu Weiyi,Wang Xin,Cong Lili,Xu Shuping
Matrix metalloproteinase-9 (MMP-9) plays a crucial role in cancer cell invasion, metastasis, and tumor growth. However, the precise and sensitive detection of MMP-9 remains a significant challenge, impeding a deeper understanding of its multifaceted roles in cancer progression. This study developed a novel surface-enhanced Raman scattering (SERS) sensing strategy based on DNA bending for ultrasensitive, highly selective detection of MMP-9. In the sensing system, MMP-9 aptamers and methylene blue–labeled complementary DNA were co-modified on a magnetic/plasmonic bead to fabricate a SERS probe. When target MMP-9 was present, the aptamer bound to the target and detached from the SERS nanoprobe, and the complementary DNA chain modified with methylene blue, due to its bending and the resultant closer proximity to the SERS nanoprobe surface, led to enhanced SERS signals. Meanwhile, cell experiments have confirmed that intensified hypoxia can directly lead to a significant increase in MMP-9 expression. Due to the high affinity between the aptamer and the target, this sensing system has high selectivity. The detection limit arrives at 3.67 ng/mL. We applied this sensor to determine MMP-9 in patient sera and obtained consistent results with the standard method. This strategy is expected to develop into a new method for diagnosing specific cancers related to MMP-9 and for evaluating the occurrence, development, and extent of cancer metastasis. Graphical abstract
The alternative text for this image may have been generated using AI.
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PubMed
高影响因子
MICROCHIMICA ACTA
·
2026
·Vol. 193
·期 6
·417
·
IF 5.4
Xue Huiling,Cheng Guorong,Zhang Bing,Zhan Ying,Zhao Zhihuan,Lin Jianying,Cheng Yan
Carbohydrate antigen 19 − 9 (CA19-9) is a critical biomarker in clinical oncology, as its serum concentration is closely associated with tumor detection and therapeutic monitoring. In this study, a novel colorimetric immunoassay was developed for the sensitive detection of CA19-9, utilizing g-C₃N₄/Ag₂S nanocomposites as a photoactive signal probe. The nanocomposites, synthesized via a hydrothermal method, demonstrated strong absorption in the visible region and high photocatalytic activity. In the immunoassay, antibody-functionalized magnetic beads were used to capture the target antigen, followed by labeling with detection probes based on the g-C₃N₄/Ag₂S nanocomposites. Under visible light irradiation, the nanocomposites catalyzed the oxidation of 3,3’,5,5’-tetramethylbenzidine (TMB), producing a distinct color change that was quantitatively analyzed via UV–vis spectroscopy. The method exhibited a wide linear detection range from 4.0 to 400 U/mL, with a detection limit of 1.86 U/mL, and demonstrated excellent specificity, stability, and reproducibility. Successful application to human serum samples further validated the reliability and practical utility of the assay, highlighting its promising potential for clinical cancer diagnosis and screening. Graphical abstract The alternative text for this image may have been generated using AI.
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PubMed
高影响因子
COLLOIDS AND SURFACES A-PHYSICOCHEMICAL AND ENGINEERING ASPECTS
·
2026
·
IF 5.4
Weiyong Hong,Haoyu Zhang,Xiuyuan Xia,Mengqian Li,Xugang Ji,Fangyuan Guo,Junbo Cai
PEGylation strategy determines protein corona composition on nanoparticles. • Surface heterogeneity leads to distinct corona-mediated cellular interactions. • Protein corona differentially affects and macrophage uptake tumor targeting. • Surface-dependent corona formation alters in vivo therapeutic efficacy. Active-targeting nanocarriers are promising for precision cancer therapy, but protein corona formation upon blood contact masks targeting ligands and triggers rapid clearance. Here, we investigate how PEG surface architecture (chemical grafting vs. physical coating) modulates protein corona formation and therapeutic outcomes. Using a star-shaped polymer (PET-PCL) functionalized with an MMP-9-targeted and responsive dual-functional peptide, we engineered two types of NPs with similar bulk properties but different surface poly (ethylene glycol) (PEG) configurations: chemical grafting versus physical coating. Quantitative proteomic analyses revealed that serum incubation led to markedly different protein corona profiles. Notably, chemically grafted NPs exhibited significantly lower adsorption of corona, effectively mitigating opsonin-mediated clearance and preserving targeting functionality. Consequently, these corona-coated NPs demonstrated superior cellular internalization in lung cancer cells (A549) and reduced phagocytosis by macrophages (RAW264.7) compared to their physically coated counterparts. Furthermore, in vivo biodistribution studies in A549 tumor-bearing mice confirmed that chemically grafted NPs achieved enhanced tumor accumulation, which corresponded with favorable antitumor activity. Collectively, these findings elucidate the critical role of surface PEG architecture in regulating protein corona formation and demonstrate that tailored surface engineering can harmonize stealth and targeting functionalities. This work provides a rational design strategy for next-generation targeted nanomedicines aimed at overcoming biological barriers in complex physiological environments. Download: Download high-res image (227KB) Download: Download full-size image
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高影响因子
SURGICAL LAPAROSCOPY ENDOSCOPY & PERCUTANEOUS TECHNIQUES
·
2019
·Vol. 29
·期 6
·426
·
IF 1.345
Lyu Yunxiao,Wang Bin,Cheng Yunxiao,Xu Yueming,Du Weibing
Background: Postendoscopic retrograde cholangiopancreatography (ERCP) pancreatitis (PEP) is one of the most common complications after ERCP. The optimal drugs for reducing the risk of PEP are still unclear. This study aimed to compare the efficacy of 9 major drugs used worldwide for the prevention of PEP through a network meta-analysis. Methods: We conducted a systematic search of the literature up to October 2018 on PubMed, Embase, Web of Science, the Cochrane Central Library, and ClinicalTrials.gov. Randomized controlled trials (RCTs) comparing allopurinol, diclofenac, gabexate (GAB), glyceryl trinitrate (GTN), indomethacin, nafamostat, octreotide, somatostatin, and ulinastatin for protection against PEP were included. Results: Eighty-six randomized controlled trials involving 25,246 patients were included in this network meta-analysis. Results indicated that diclofenac, GAB, GTN, indomethacin, somatostatin, and ulinastatin were more effective than placebo with odds ratios ranging between 0.48 (95% credible interval, 0.26-0.86) for GAB and 0.61 (0.39-0.94) for somatostatin. However, allopurinol, nafamostat, and octreotide showed similar efficacy as placebo in reducing the risk of PEP. No significant differences were found in the efficacy between diclofenac, GAB, GTN, indomethacin, somatostatin, and ulinastatin. In terms of prognosis, GAB may be the most effective treatment (surface under the cumulative ranking curve=70.6%) and the least effective was octreotide (surface under the cumulative ranking curve=28%). Conclusions: Although our analysis suggests that GAB may be the most effective drug in preventing PEP, the limitations of our study warrants more high-quality head-to-head trials of these clinical drugs in the future.
研究领域:
消化病学
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临床药理学
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荟萃分析
·
内镜
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DOI
高影响因子
危险品化学品经营许可证（带存储）