LWT-FOOD SCIENCE AND TECHNOLOGY
·
2025
·
IF 6.6
Jiagang Guo,Yuhan Wu,Siyu Sun,Qian Zhu,Jingjing Du,Jiayu Gu,Song Yang,Jian Jiang
This study aims to evaluate the physicochemical, functional, and antioxidant properties of bamboo shoot protein (BSP) produced by alkali isoelectric precipitation and ultrasound- and enzyme-assisted alkali extraction methods. The extraction method significantly affected the properties of BSP. Both ultrasound- and enzyme-assisted extractions resulted in higher protein recovery yield and purity compared to alkali isoelectric precipitation. SDS-PAGE results indicated that BSP exhibited low molecular weight (12–65 kDa). However, ultrasonication and enzymatic digestion disrupted the secondary and tertiary structures of proteins, leading to smaller particle sizes and higher levels of β-turn and random coil content. In this study, BSP isolated using enzymatic-assisted alkali extraction (E-BSP) showed the highest solubility, foaming stability, emulsion activity index, water-holding capacity, and fat absorption capacity. Moreover, E-BSP exhibited the lowest IC 50 values for scavenging hydroxyl radicals (2.90 mg/mL) and ABTS + radicals (0.39 mg/mL). These findings provide a basis for the high-value utilization of bamboo shoots in the food industry.
研究领域:
食品科学
·
蛋白质化学
·
功能食品
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产品关联
DOI
高影响因子
Advanced Healthcare Materials
·
2024
·
IF 10
Ke Yang,Li Wang,Jinyi Chen,Zefeng Wang,Junqin Li,Xi Chen,Shuting Fu,Luo Hai,Le Deng,Dinggeng He
High H 2 O 2 levels are widely present at the infection sites or in the biofilm microenvironment. Herein, hemin with peroxidase-like catalytic activity and its substrate, 2,2′-azino-bis(3-ethylbenzothiazoline-6-sulfonic acid) (ABTS), are simultaneously introduced into a liposomal nanoparticle containing thermosensitive 2,2′-azobis[2-(2-imidazolin-2-yl) propane] dihydrochloride (AIBI)-loaded bovine serum albumin (BAG), rationally constructing an H 2 O 2 -activatable liposomal nanobomb (Lipo@BHA) for combating biofilm-associated bacterial infections with high performance. In the presence of H 2 O 2 , hemin can catalyze the conversion of ABTS into its oxidized form (ABTS· + ) with strong near-infrared (NIR) absorption, which produces photonic hyperpyrexia to cause the decomposition of AIBI into oxygen-independent alkyl radicals (·R) and nitrogen (N 2 ) microbubbles. The former not only directly damage bacterial cells but also significantly accelerates the oxidization of ABTS to ABTS· + for augmenting photothermal-triggered generation of ·R. Interestingly, the released N 2 can induce transient cavitation to rupture lysosomal nanoparticle and improve the biofilm permeability, thereby enhancing the antibiofilm effect of Lipo@BHA. The proposed Lipo@BHA exhibits satisfactory multi-mode combination antibacterial properties. Through endogenous H 2 O 2 -activated cascade reaction, Lipo@BHA achieves remarkable hypoxia-irrelevant ·R therapy of biofilm-associated wound infections with low cytotoxicity and good in vivo biosafety. Therefore, this work presents a versatile H 2 O 2 -activatable cascade ·R generation strategy for biofilm-specific therapeutic applications.
研究领域:
纳米技术
·
生物医学工程
·
药学
·
微生物学
·
材料科学
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产品关联
DOI
PubMed
高影响因子
JOURNAL OF WOOD CHEMISTRY AND TECHNOLOGY
·
2018
·
IF 1.418
Hui Chen,Wei Song,Kun-Kun Sun,He-Wei Du,Shu-Dong Wei
This study aimed to investigate the structural features, antioxidant activity, tyrosinase inhibitory effect, and mechanism of proanthocyanidins from leaf and fruit of Leucaena leucocephala. MALDI-TOF-MS, thiolysis coupled with HPLC-ESI-MS, and 13C-NMR confirmed that these proanthocyanidins were complex mixtures of propelargonidins, procyanidins, and prodelphinidins. (Epi)catechin, (epi)gallocatechin, (epi)catechin gallate, and (epi)gallocatechin gallate were the main constitutive units. The findings obtained from enzyme analysis revealed that the proanthocyanidins had inhibitory effects on both monophenolase and diphenolase of tyrosinase. The IC50 values of leaf and fruit proanthocyanidins were 73.5 ± 2.7 and 52.3 ± 3.5 µg/mL for monophenolase activity, and 27.2 ± 1.4 and 16.1 ± 1.1 µg/mL for diphenolase activity, respectively. The inhibition of diphenolase by proanthocyanidins were proved to be reversible and mixed type. In addition, it was also found from various antioxidant methods that the proanthocyanidins in leaf and fruit possessed potent DPPH radical scavenging activity with IC50 values of 103.4 ± 0.8 and 87.8 ± 1.1 µg/mL, ABTS radical scavenging activity with IC50 values of 72.9 ± 0.4 and 65.7 ± 0.9 µg/mL, and ferric reducing antioxidant power with FRAP values of 3.74 ± 0.03 and 4.02 ± 0.15 mmol AAE/g, respectively. The results obtained suggested that the proanthocyanidins from leaf and fruit of L. leucocephala might have the potential to be exploited as natural tyrosinase inhibitors and antioxidants.
研究领域:
药用植物学
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生物化学
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分析化学
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药理学
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产品关联
DOI
高影响因子
FOOD CHEMISTRY
·
2025
·
IF 9.8
Jiao Wu,Xiang-Tan Xu,Cheng Xing,Xin-Bao Hao,Xing-Yue Fang,Zhi-Hao Xie,Shan Zhao,Jing-Lin Gao,Lu Xu,Shi-Jie Wang
Honey has been recognized for its role in disease prevention through nutritional modulation. In this study, we comprehensively assessed the physicochemical parameters, metabolic profile, antioxidant and anti-inflammatory activities of multifloral honeys produced by Apis cerana cerana in Sansha City (SS), Hainan Province. Metabolomic analysis identified SS honey had multiple bioactive compounds known for antioxidant and anti-inflammatory properties. Compared to A. cerana cerana honey from central regions of Hainan Province, SS honey showed higher levels of TFC (113.80 mg RE/100 g) and TPC (45.86 mg GAE/100 g). Furthermore, SS honey demonstrated greater antioxidant activity, as evidenced by FRAP (1503.16 μmol TE/kg), DPPH IC 50 (36.13 mg/mL), and ABTS IC 50 (11.03 mg/mL). SS honey significantly reduced the levels of cyclooxygenase-2 (COX-2), interleukin-1β (IL-1β), interleukin-6 (IL-6), inducible nitric oxide synthase (iNOS), and tumor necrosis factor-α (TNF-α) in lipopolysaccharide (LPS)-induced RAW 264.7 cells, along with suppressing the mRNA expression of these inflammatory markers.
研究领域:
养蜂学
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生物化学
·
药理学
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营养科学
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代谢组学
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产品关联
DOI
PubMed
高影响因子
Journal of Future Foods
·
2025
·
IF 7.2
Lei Dou,Zimu Zhang,Xiufang Huang,Yaobing Chen,Kai Luo,Jianquan Kan
Considering that the peptide from Idesia polycarpa Maxim.cake meal polypeptide (CIP) is a novel antioxidant agent, this study aimed to develop an efficient and safe food film by incorporating the CIP agent into a gelatin matrix. The films with different concentrations of CIP were characterised and applied in the preservation of nectarines. The results indicated that the CIPF/3% fraction exhibited significant moisture barrier properties, with a water vapor permeability (WVP) of only 8.12 % ± 0.09 %. Additionally, it demonstrated excellent mechanical and antioxidant properties, with scavenging capacities of 76.31 % ± 0.597 %, 65.98 % ± 0.444 %, and 60.98 % ± 0.231% for DPPH, ABTS, and hydroxyl radicals, respectively, surpassing those of other fractions. In nectarine freshness preservation, the Coating/3% fraction showed the best performance. It significantly reduced the weight loss rate of nectarines, improved fruit firmness, maintained the pH value, and minimized nutrient losses, including ascorbic acid and Total soluble sugars (TSS). Furthermore, it effectively preserved the bright surface color of nectarines and extended their shelf life. In summary, the specific environmental protection, safety, and preservation qualities of the CIPF/Coating in food packaging suggest a promising potential for its application.
研究领域:
食品科学
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高分子科学
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抗氧化技术
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食品保鲜
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材料表征
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可食用涂层
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农业副产物利用
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产品关联
DOI
高影响因子
Food & Nutrition Research
·
2020
·
IF 3.647
Hai-Yan Wang,Jin-Ge Zhao,Yu-Qing Zhang
The green cocoon shell of a novel variety of silkworm, Bombyx mori, is rich in two types of quercetin and kaempferol flavonoids. The aim of this study was to identify these flavonoids in the ethanolic extract (EE) from green cocoons and develop EE applications in healthy foods. The experimental results indicated that the amount of total amino acids in EE was 27.06%. The flavonoids in EE are presented in quercetin and kaempferol glycosides. The total amount of the two aglycones was 33.42 ± 0.08 mg/g. The IC 50 values of 2,2-diphenyl-1-picrylhydrazyl (DPPH), 1,2’-azino-bis (3-ethylbenzthiazoline-6-sulphonicacid) (ABTS), and hydroxyl radical scavenging abilities were 296.95 ± 13.24 μg/mL, 94.31 ± 9.13 μg/mL, and 9.21 ± 0.15 mg/mL, respectively. The IC 50 values of the inhibitory activities of α-amylase and α-glucosidase were 37.57 ± 6.45 μg/mL and 212.69 ± 22.94 μg/mL, respectively. EE also reduced the level of reactive oxygen species (ROS) and oxidative stress in L02 cells induced by high glucose levels. It also effectively decreased the content of 8-hydroxyl deoxyguanosine (8-OHdG), nuclear factor κB (NF-κB), and tumour necrosis factor alpha (TNF-α) in cells with a good dose effect. These results clearly indicated that the flavonoid-rich EE with excellent antioxidant and glucosidase inhibition abilities significantly reduced the damage to cells caused by oxidative stress and inflammatory reactions. It is suggested that EE might serve as useful functional foods for the treatment of related diseases induced by oxidative stress such as diabetes mellitus.
研究领域:
药理学
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生物化学
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营养学
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食品科学
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产品关联
DOI
PubMed
高影响因子
Frontiers in Nutrition
·
2022
·
IF 6.59
Yuxiao Wu,Yu Liu,Jiayi Wu,Kaiqi Ou,Qian Huang,Junxian Cao,Tao Duan,Lin Zhou,Yufang Pan
This study aimed to establish a bidirectional fermentation system using Tremella fuciformis and Acanthopanax trifoliatus to promote the transformation and utilization of the synthesized antioxidant metabolites from fermentation supernatant. The effect of fermentation conditions on the total phenolic content was investigated using response surface methodology in terms of three factors, including temperature (22-28°C), pH (6-8), and inoculum size (2-8%, v/v). The optimized fermentation parameters were: 28°C, pH 8, and an inoculum size of 2%, which led to a maximum total phenolic content of 314.79 ± 6.89 μg/mL in the fermentation supernatant after 24 h culture. The content of total flavonoids and polysaccharides reached 78.65 ± 0.82 μg/mL and 9358.08 ± 122.96 μg/mL, respectively. In addition, ABTS+, DPPH⋅, and ⋅OH clearance rates reached 95.09, 88.85, and 85.36% at 24 h under optimized conditions, respectively. The content of total phenolics, flavonoids and polysaccharides in the optimized fermentation supernatant of T. fuciformis - Acanthopanax trifoliatus increased by 0.88 ± 0.04, 0.09 ± 0.02, and 33.84 ± 1.85 times that of aqueous extracts from A. trifoliatus , respectively. Simultaneously, 0.30 ± 0.00, 0.26 ± 0.01, and 1.19 ± 0.12 times increase of antioxidant activity against ABTS+, DPPH⋅, and ⋅OH clearance rates were observed, respectively. Additionally, the metabolite composition changes caused by fermentation were analyzed using ultra-high performance liquid chromatography-tandem mass spectrometry (UPLC-MS/MS) based on untargeted metabolomics and the phytochemical profile of fermentation supernatant differentiated significantly based on unsupervised principal component analysis (PCA) during fermentation from 24 to 96 h. Furthermore, a significant increase in antioxidant phenolic and flavonoid compounds, such as ellagic acid, vanillin, luteolin, kaempferol, myricetin, isorhamnetin, and (+)-gallocatechin, was observed after fermentation. Thus, these results indicated that the fermentation broth of T. fuciformis and A. trifoliatus had significant antioxidant activity, and may have potential application for health products such as functional beverages, cosmetics, and pharmaceutical raw materials.
研究领域:
微生物学
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药理学
·
生物化学
·
食品科学
·
代谢组学
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产品关联
DOI
PubMed
高影响因子
Journal of Functional Foods
·
2023
·
IF 5.6
Chunlei Li,Weijuan Huang,Hang Zheng,Hui Shi,Sixue Bi,Liyan Song,Jianhua Zhu,Rongmin Yu,Yurong Wang
A novel acidic homogeneous polysaccharide (AIPW50-1) with a molecular weight of 214.5 kDa was isolated by hot water extraction and several column chromatographic purification methods from Arca inflata Reeve. The repeating structural units of AIPW50-1 were elucidated by the combined chemical and instrumental analyses. AIPW50-1 is composed of glucose, glucuronic acid and galactose with molar ratio of 18.06: 1.00: 1.56. The backbone of AIPW50-1 consisted of →4)-α-D-GlcpA-(1→, →4)-α-D-Glcp-(1→ and →4,6)-α-D-Glcp-(1→ with two branches of α-D-Glcp-(1→ and →6)-β-D-Galp-(1→ terminated with α-D-Glcp-(1→, respectively. The antioxidant assay demonstrated that AIPW50-1 possessed strong ABTS free radical scavenging activity and prominently stimulated the secretion of a cytotoxic molecule (NO), cytokines (TNF-α and IL-6) by murine RAW 264.7 macrophages. The in vivo antioxidative effect of AIPW50-1 was further evaluated using Caenorhabdiyis elegans oxidative stress model. AIPW50-1 could prolong the lifespan, improve locomotory behavior and reproductive ability, and significantly enhance intracellular activities of catalase (CAT), superoxide dismutase (SOD) and glutathione peroxidase (GSH-Px) of C. elegans by 19.80 %, 17.05 % and 17.22 % at 4 mg/mL. These results suggested that AIPW50-1 has the potential to be used as a functional additive with antioxidant and immunomodulatory effects.
研究领域:
海洋生物学
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免疫学
·
生物化学
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药理学
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产品关联
DOI
高影响因子
INTERNATIONAL JOURNAL OF BIOLOGICAL MACROMOLECULES
·
2024
·
IF 7.7
Qianzi Chen,Xuekui Nie,Wangxiang Huang,Chen Wang,Ren Lai,Qiumin Lu,Qiyi He,Xiaodong Yu
Chicken meat processing generates a substantial number of byproducts, which are either underutilized or improperly disposed. In this study, we employed in silico approaches to identify antioxidant peptides in chicken liver byproducts. Notably, the peptide WYR exhibited remarkable 2,2-azino-bis-(3-ethylbenzothiazoline-6-sulfonic acid) diammonium salt (ABTS) radical scavenging activity with an IC 50 of 0.13 ± 0.01 mg/mL and demonstrated stability under various conditions, including thermal, pH, NaCl, and simulated gastrointestinal digestion. Molecular docking analysis revealed significant hydrogen bonding interactions, while molecular dynamics showed differential stability with ABTS and 2,2-Diphenyl-1-picrylhydrazyl (DPPH). WYR exhibited improved stress resistance, decreased levels of reactive oxygen species (ROS), elevated the activities of superoxide dismutase (SOD) and catalase (CAT), and modulated the expression of crucial genes through the insulin/insulin-like growth factor (IIS) signaling pathway, mitogen-activated protein kinase (MAPK), and heat shock transcription factor-1 (HSF-1) pathways. These effects collectively contributed to the extension of Caenorhabditis elegans ' lifespan. This study not only provides an effective method for antioxidant peptide analysis but also highlights the potential for enhancing the utilization of poultry byproducts.
研究领域:
食品科学
·
生物技术
·
分子生物学
·
衰老研究
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产品关联
DOI
PubMed
高影响因子
TheScientificWorldJOURNAL
·
2014
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IF 1.219
Yujuan Zhang,Ziwen Luo,Dongmei Wang,Fengyuan He,Dengwu Li
The ethanol crude extracts (ECE) and their subfractions from Zanthoxylum bungeanum leaves were prepared and their phytochemical profiles and antioxidant and antimicrobial activities were investigated. Moreover, the effective HPLC procedure for simultaneous quantification of twelve compounds in Z. bungeanum leaves was established. The correlation between the phytochemicals and antioxidant activity was also discussed. The ethyl acetate fraction (EAF) had the highest total phenolic (97.29 mmol GAE/100 g) and flavonoid content (67.93 mmol QE/100 g), while the greatest total alkaloid content (4.39 mmol GAE/100 g) was observed in the chloroform fraction (CF). Twelve compounds were quantified by RP-HPLC assay. EAF exhibited the highest content of quercitrin, kaempferol-3-rhamnoside, quercetin, sesamin, and nitidine chloride (125.21, 54.95, 24.36, 26.24, and 0.20 mg/g); acetone fraction (AF) contained the highest content of chlorogenic acid, rutin, hyperoside, and trifolin (5.87, 29.94, 98.33, and 31.24 mg/g), while kaempferol-3-rhamnoside, xanthyletin, and sesamin were rich in CF. EAF and AF exhibited significant DPPH, ABTS radical scavenging abilities and reducing power (FRAP), whereas CF exhibited significant antifungal activity. Moreover, EAF also showed stronger antibacterial activity. In conclusion, Z. bungeanum leaves have health benefits when consumed and could be served as an accessible source for production of functional food ingredients and medicinal exploration.
研究领域:
药用植物学
·
植物化学
·
食品科学
·
药物化学
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产品关联
DOI
PubMed
高影响因子
Scientific Reports
·
2017
·Vol. 7
·期 1
·1-12
·
IF 4.259
Jiang Bin,Zhang Xiaoqing,Yuan Yongqiang,Qu Yuxiao,Feng Zhibiao
An aqueous two-phase system (ATPS) consisting of poly(ethylene glycol-ran-propylene glycol) monobutyl ether (UCON)/phosphate was developed for the separation of the antioxidant peptides from pepsin hydrolysate of Whey Protein Isolate (WPI). The efficiency of the separation was evaluated based on the DPPH radical scavenging activity, ABTS radical scavenging activity and ferric reducing antioxidant power (FRAP) of the separated peptides. The effects of some parameters on the partition of antioxidant peptides were investigated. An efficient separation of antioxidant peptides was achieved using ATPS with pH of 4.0, 4 mL of UCON solution (40%, w / w ), 4 mL of KH 2 PO 4 solution (15.5%, w / w ), 2 mL of WPI hydrolysate and 0.40 g/10 mL of NaCl. Reversed-phase high-performance liquid chromatography (RP-HPLC), amino acid analyzer and matrix-assisted laser desorption ionization time-of-flight mass spectrometry (MALDI-TOF MS) were used to characterize the purified peptides separated by the ATPS. The peptides in top phase were less polar than those in bottom phase. More antioxidative and hydrophobic amino acids were extracted to the top phase of ATPS, and the peptides with the amino acid sequences with antioxidant activities moved to the top phase as well. In conclusion, antioxidant peptides were successfully separated from the WPI hydrolysate by UCON/phosphate ATPS.
研究领域:
生物化学
·
食品科学
·
分析化学
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产品关联
DOI
PubMed
高影响因子
ACS Applied Materials & Interfaces
·
2024
·
IF 8.3
Chen Chen,Fructueux Modeste Amona,Junhao Chen,Xiaohan Chen,Yongding Ke,Shuangcheng Tang,Jinming Xu,Xi Chen,Yipeng Pang
Wound healing is a complex biological process that can trigger inflammation and oxidative stress and impair myofibrillogenesis and angiogenesis. Several advanced wound-dressing nanocomposite materials have been designed to address these issues. Here, we designed a new multifunctional styrene–ethylene–butylene–styrene/silver nanowire (SEBS/AgNWs)-based nanocomposite film with antimicrobial, antioxidant, and anti-inflammatory properties to promote wound healing. The porous morphological structure of SEBS/AgNWs enhances their antimicrobial, antioxidant, and anti-inflammatory properties. SEBS/AgNWs significantly inhibited the growth of Staphylococcus aureus, methicillin-resistant Staphylococcus aureus, and Escherichia coli strains, effectively wiping out ABTS•+, DPPH•, hydrogen peroxide (H2O2), and hydroxyl (•OH) radicals, showing their effective ROS-scavenging properties. It further showed significant antioxidant properties by increasing the levels of enzyme-like catalase (CAT), superoxide dismutase (SOD), and glutathione (GSH), while decreasing malonaldehyde (MDA) levels. Additionally, SEBS/AgNWs reduced the expression of interleukin-1β (IL-1β), IL-6, and tumor necrosis factor-α (TNF-α), while increasing levels of transforming growth factor- β (TGF-β), vascular endothelial growth factor-A (VEGF), and CD31 in wound healing. This suggests that applying a multifunctional nanoplatform based on SEBS/AgNWs could enhance wound healing and improve patient outcomes in wound care management.
研究领域:
材料科学
·
生物医学工程
·
纳米技术
·
伤口护理
·
药学
✓ 已验证
DOI
PubMed
高影响因子
危险品化学品经营许可证（带存储）