ANALYTICA CHIMICA ACTA
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2026
·
IF 6.1
Jian Zhang,Jin Wang,Jiarong Wei,Jiaxin Li,Maofang He,Chunye Liu
AuNP-mediated oriented immobilization enhances AChE loading capacity by 8.6-fold. • Rotating-splicing design improves operational convenience and enzyme reusability. • AuNPs@enzyme OIMERs show superior performance: K m = 0.051 ± 0.001 mM and RSD < 1.0%. • AuNPs@enzyme OIMERs retain 87.0% after 80 runs and 93.3% after 40 days storage. • System enables bioassay-guided fractionation via visual/quantitative evaluation. Background Acetylcholinesterase (AChE) inhibitors are key therapeutic agents for neurological disorders such as Alzheimer's disease, yet conventional screening of natural product inhibitors are often time-consuming, instrument-dependent, and limited in reusability. To address this, we developed a simple, colorimetric platform based on a reusable microfluidic paper-based analytical device integrated with Au nanoparticle (AuNPs)-mediated enzyme microreactors, coupled with smartphone-based RGB values (blue (B), green (G), and red (R) channel) analysis for rapid and on-site screening of AChE inhibitors from natural products. The microreactors feature an “apple tree”-like architecture, in which AuNPs serve as the “fruits” enabling high-density oriented enzyme immobilization, enhanced substrate accessibility, and excellent reusability. Results AChE was oriented immobilized on concanavalin A-modified AuNPs surface via affinity self-assembly, achieving an 8.6-fold increase in enzyme loading compared to direct adsorption. The immobilized enzyme retained 87.0% of its initial activity after 80 consecutive assays, demonstrating excellent reusability and stability. The method showed superior repeatability with intraday, interday, and batch-to-batch relative standard deviations (RSD) of the colorimetric readouts all below 1.0%. The system enabled visual, quantitative evaluation during bioassay-guided fractionation, substantially reducing unnecessary separation steps. Moreover, half-maximal inhibitory concentration (IC 50 ) values of seven compounds from natural products were determined, correlating well with the reference UV method, confirming the reliability for practical inhibitor screening. Significance This platform offers a cost-effective, reusable, and user-friendly tool for early-stage screening of AChE inhibitors in natural extracts, with potential to accelerate the discovery of lead compounds for neurodegenerative disease therapy while minimizing resource consumption and analytical workload. Download: Download high-res image (291KB) Download: Download full-size image
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ANALYTICAL AND BIOANALYTICAL CHEMISTRY
·
2023
·
IF 4.3
Zou Rong,Cao Lei,Wu Nan,Chang Gang,Li Li,Xiao Lu,Yan Huiling,Li Hongjie,Wang Ping,Bao Ting,Zhang Xiuhua,Wang Shengfu,Wang Yaping,He Hanping
Alpha-fetoprotein (AFP) is inextricably linked to various diseases, including liver cancer. Thus, detecting the content of AFP in biology has great significance in diagnosis, treatment, and intervention. Motivated by the urgent need for affordable and convenient electronic sensors in the analysis and detection of aqueous biological samples, we combined the solution-gated graphene transistor (SGGT) with the catalytic reaction of enzyme nanoprobes (HRP-AuNPs-Ab2) to accurately sense AFP. The SGGT immunosensor demonstrated high specificity and stability, excellent selectivity, and excessive linearity over a range of 4 ng/mL to 500 ng/mL, with the lower detection limit down to 1.03 ng/mL. Finally, clinical samples were successfully detected by the SGGT immunosensor, and the results were consistent with chemiluminescence methods that are popular in hospitals for detecting AFP. Notably, the SGGT immunosensor is also recyclable, so it has excellent potential for use in high-throughput detection. Graphical abstract
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SENSORS AND ACTUATORS B-CHEMICAL
·
2023
·
IF 8.4
Fuchun Si,Xiaojing Cui,Yaping Zhang,Yifan Li,Huaixia Yang,Yanju Liu
Down-regulator of transcription 1 (DR1) is a crucial biomarker closely related to Hashimoto's Thyroiditis (HT). In this study, a highly selective electrochemical biosensor for ultrasensitive detection of DR1 was prepared by combining nanomaterials CMK-3 @AuNPs and atom transfer radical polymerization (ATRP) signal amplification strategy for the first time. Based on the specific recognition of antigen and antibody, the sensor has strong selectivity. CMK-3 @AuNPs can not only increase the conductivity of the electrode, but also provide more active sites for biological molecules, so as to effectively amplify the output signal. The FMMA monomers were polymerized on the electrode surface through ATRP reaction, which made the electrochemical signal increased significantly. Under optimal conditions, the fabricated electrochemical biosensor showed a wide detection range of 5 × 10 -4 ng/mL ∼ 5 × 10 2 ng/mL with a detection limit as low as 0.159 pg/mL. In addition, the electrochemical biosensor has good stability and anti-interference ability. Therefore, the sensor has broad prospects in the detection of biomarkers.
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MICROCHEMICAL JOURNAL
·
2023
·
IF 4.8
Miao Zhang,Yin Chen,Shi Gang Liu,Xingbo Shi
L. monocytogenes (L. m) is one of the most common causes of foodborne diseases. To enable timely monitoring of L. m contamination, a highly efficient and sensitive electrochemical biosensor was developed for the precise determination of L.m . The biosensor was prepared by sequentially modifying glassy carbon electrode (GCE) with silicon methylene blue (Si@MB), gold nanoparticles (AuNPs) and aptamer (Apt). Differential pulse voltammetry (DPV) was used to quantify L.m . This biosensor could be finished in 1.5 h, which significantly reduced the detection time. The resulting electrochemical biosensor achieved good linear (10 2 -10 7 CFU/mL) and excellent detection limit of 2.6 CFU/mL. Moreover, the novel strategy has been successfully applied to actual samples, with the recoveries ranging from 84.7% to116.0% in lettuce and from 80.0% to 110% in fresh-cut fruit. This biosensor shows great potential application in monitoring bacterial food contamination and infectious diseases.
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JOURNAL OF ALLOYS AND COMPOUNDS
·
2020
·
IF 4.65
Xing Chen,Yao Liu,Xu-Xu Ke,Rohan Weerasooriya,Heng Li,Lian-Chao Wang,Yu-Cheng Wu
Methylated mercury is a bio-magnifier that readily crosses the blood-brain barrier exerting neurotoxic effects. Therefore, the development of a rapid and in situ method of methylmercury (CH 3 Hg + ) detection is research priority. We report a sensitive electrochemical method for the detection of CH 3 Hg + using a modified glassy carbon electrode with Au nanoparticles-graphitic carbon nitride (AuNPs/mpg-C 3 N 4 ). The AuNPs/mpg-C 3 N 4 nanocomposites was synthesized by photochemical method and characterized by X-ray diffraction (XRD), Scanning electron microscope (SEM), Transmission electron microscope (TEM), X-ray photoelectron spectroscopic (XPS), Fourier transform infrared spectrometer (FTIR). The results showed that Au nanoparticles were successfully loaded on the surface of mpg-C 3 N 4 and dispersed uniformly. AuNPs/mpg-C 3 N 4 combined the excellent catalytic properties of AuNPs and unique structure of mpg-C 3 N 4 to improve the electrochemical sensing performance of CH 3 Hg + . The AuNPs/mpg-C 3 N 4 GCE show a high sensitivity for CH 3 Hg + determination using Differential pulse stripping voltammetry (DPSV). The CH 3 Hg + calibration curve is linear within 1 μg/L ∼25 μg/L. The sensitivity and detection limit of CH 3 Hg + is 0.285 μA/μg −1 L and 0.103 μg/L (S/N = 3), respectively. The Hg 2+ shows chemical interference in CH 3 Hg + detection, which is minimized by the addition of SnCl 2 or Diethylene triamine pentaacetic acid (DTPA). We also determined the CH 3 Hg + concentration of natural waters with a ±106% spike recovery. A newly developed AuNPs/mpg-C 3 N 4 modified GCE sensor provides a promising platform for CH 3 Hg + detection in natural water.
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ANALYTICAL AND BIOANALYTICAL CHEMISTRY
·
2020
·Vol. 413
·期 3
·813-820
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IF 3.637
Dou Nannan,Qu Jianying
In this work, a hybrid of multiwalled carbon nanotubes, nanogold, and reduced graphene (rGO/AuNPs/MWCNTs) was synthesized rapidly with an easy method, and then combined with chitosan (CS), which was fixed on a glassy carbon electrode (GCE) to construct a new kind of electrochemical sensor to simultaneously determine 4-aminophenol (4-AP) and acetaminophen (AC). When detecting 4-AP and AC simultaneously, the linear range is 0.12~12 μM for acetaminophen and 0.05~25 μM for 4-aminophenol; the detection limit is 42 nM for acetaminophen and 2.95 nM for 4-aminophenol. Compared with previously related reports, the proposed sensor has an excellent electrocatalytic performance for the redox of 4-AP and AC, which can effectively determine 4-AP and AC simultaneously in actual samples and has potential application prospect.
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JOURNAL OF ELECTROANALYTICAL CHEMISTRY
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2020
·
IF 3.807
Qixia Guan,Hao Guo,Rui Xue,Mingyue Wang,Xin Zhao,Tian Fan,Wenhu Yang,Mengni Xu,Wu Yang
An electrochemical sensing platform for simultaneous determination of dopamine (DA) and uric acid (UA) is constructed based on covalent organic framework materials (IL COF-1), aminofunctionalized carbon nanotubes (MWCNT-NH 2 ), and gold nanoparticles (AuNPs). The prepared sensor shows a good performance in detection of DA and UA due to porosity, large specific surface area, high conductivity and more active sites of the composite modified material. Under optimized experimental conditions, the constructed electrochemical sensor has sensitive response and good linearity to both DA and UA. Their wide linear ranges are 0.7–108 μM and 0.97–200 μM, and the detection limits are 0.21 μM and 0.29 μM (S/N = 3), respectively. The developed method has been successfully employed to determine the contents of DA and UA in dopamine injection and human urine samples. It has a satisfactory recovery rate, and the relative standard deviations (RSD) of three parallel determinations are all below 5.0%. In comparison, the constructed sensor platform has superior performance including low cost, simple structure, wide linear range and high sensitivity than some traditional methods, which is expected to be widely used in real sample analysis.
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MICROCHIMICA ACTA
·
2020
·Vol. 187
·期 5
·1-10
·
IF 6.232
Yang Yan,Li Guoliang,Wu Di,Wen Aying,Wu Yongning,Zhou Xuxia
β-Cyclodextrin-functionalized magnetic covalent organic framework (Fe 3 O 4 @COF@Au-β-CD) was developed as sorbent for magnetic solid phase extraction of trace sulfonamides in meat samples prior to HPLC-MS/MS analysis. The sorbent was synthesized by loading gold nanoparticles onto a Fe 3 O 4 @COF surface and then functionalized by thiolated-β-cyclodextrin immobilization via Au-S bonding formation. The prepared composite material was employed for sulfonamides extraction. The main parameters were optimized to obtain the best extraction efficiency. The experiments of adsorption kinetics were carried out to investigate the adsorption mechanism. Results showed the pseudo-second-order kinetic was better fitted with the adsorption kinetics of sulfonamides. Under optimized conditions, the magnetic solid phase extraction-HPLC method showed good linearity ( R 2 ≥ 0.9936), and the limits of detection were in the range of 0.8–1.6 μg kg −1 . The proposed method was successfully used for quantitation of sulfonamides in real samples. The recoveries ranged from 78.9 to 112.0% with relative standard deviations (RSDs) < 10% ( n = 5). The proposed method exhibited great potential for enrichment and determination of sulfonamides in many other food or environment samples.
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JOURNAL OF INDUSTRIAL AND ENGINEERING CHEMISTRY
·
2018
·
IF 4.841
Yuanyuan Liu,Cui Du,Chen Zhou,Shengyang Yang
The hierarchical AuNPs/Cd 0.5 Zn 0.5 S hybrids are directly fabricated via a facile one-step in-situ hydrothermal method. The as-prepared AuNPs/Cd 0.5 Zn 0.5 S hybrids demonstrate superior photocatalytic performance toward hydrogen production under visible light irradiation. The hydrogen evolution rate of the 5 wt% AuNPs/Cd 0.5 Zn 0.5 S synthesized with in-situ hydrothermal method can be 7.1 times greater than that of pure Cd 0.5 Zn 0.5 S and as much as 2.5 times of 5 wt% AuNPs loaded Cd 0.5 Zn 0.5 S synthesized with photodeposition method. Systematical investigations reveal that the enhanced photocatalytic performance of the one-step in-situ prepared AuNPs/Zn 0.5 Cd 0.5 S can be attributed to the inherent SPR effect and favorable electron transfer properties of AuNPs, as well as the rational hierarchical nanoarchitectures that allow AuNPs to be uniformly incorporated into Zn 0.5 Cd 0.5 S matrix. This one-step in-situ fabrication method provides a simple and efficient route to synthesize well-defined heterocatalysts.
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JOURNAL OF BIOMEDICAL MATERIALS RESEARCH PART A
·
2018
·Vol. 106
·期 12
·3239-3247
·
IF 3.231
Xiuzhen Wan,Pengcheng Liu,Xingxing Jin,Xuanxuan Xin,Pengfei Li,Jiang Yuan,Jian Shen
Nitric oxide (NO)-generating materials are beneficial for vascular tissue engineering (VTE) scaffold because the produced NO would enhance endothelial cells viability while inhibit smooth muscle cell (SMC) proliferation and reduce platelet adhesion, resulting in ideal hemocompatibility and endothelialization. Herein, poly(ε-caprolactone) (PCL)/keratin biocomposite mats were first fabricated, followed by in situ gold (Au) nanoparticles loading to afford PCL/keratin/AuNPs mats. These mats were characterized using field emission scanning electron microscopy (FE-SEM), X-ray photoelectron spectroscopy (XPS), X-ray diffraction (XRD), and thermogravimetric analysis (TGA). The PCL/keratin/AuNPs mats were demonstrated to be capable of catalyzing NO release in the mimicked blood microenvironments. The generated NO could enhance human umbilical vein endothelial cell growth and inhibit human umbilical arterial SMC viability. In addition, these mats maintained the antibacterial activity of Au nanoparticles with good blood compatibility. Taken together, these keratin-based composite mats have potential usage in the VTE. © 2018 Wiley Periodicals, Inc. J Biomed Mater Res Part A: 106A: 3239–3247, 2018.
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Journal of Biomedical Nanotechnology
·
2018
·Vol. 14
·期 10
·1688-1694
·
IF 5.068
Lai Yuxuan,Deng Yan,Yang Gaojian,Li Song,Zhang Chuanxiang,Liu Xueying
A novel molecular imprinted polymer (MIP) electrochemical sensor was successfully fabricated for sensitive detection of carcinomaembryonic antigen (CEA). We used CEA as template, dopamine (DA) as imprinted monomers. Through controlling electropolymerization, a "PDA-CEA"complex was achieved.
After elution, the specific cavities adsorbed the target molecules. In addition, polythionine (PTh) and AuNPs were applied as the electrode modifying materials to enhance electron transfer rate and improve detection signal. Using differential pulse voltammetry (DPV) detection, the peak current
decreased with the increase in concentration of CEA, and the linear response range of the MIP sensor was from 0.001 ng/mL to 1000 ng/mL with the detection limit as low as 0.2589 pg/mL. The MIP sensor had a low sample consumption, good stability, and high sensitivity, and could become a new
promising method for the detection of CEA. Furthermore, this MIP sensor was demonstrated in testing CEA in human serum sample with satisfactory results.
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Journal of the Taiwan Institute of Chemical Engineers
·
2017
·
IF 4.217
Xinna Zhao,Tifeng Jiao,Xilong Ma,Hao Huang,Jie Hu,Yuan Qu,Jingxin Zhou,Lexin Zhang,Qiuming Peng
The diamond-based hierarchical AuNPs-modified nanocomposites materials were prepared via the synthesis of diamond@graphite building block and polyallylamine hydrochloride (PAH)/polyacrylic acid (PAA) layer-by-layer (LbL) assembled strategy. And the modified gold nanoparticles were carried out by the reduction of aqueous HAuCl 4 solution using the reductant NaBH 4 . The obtained layer-by-layer nanocomposites demonstrate obvious advantages such as high specific surface area, pore diameter and pore volume, providing a higher chance of gold nanoparticles accessible to the reactants. Thus the core–shell nanostructures from of LbL-assembled and diamond-based composite materials are beneficial to improve the catalytic capacity of gold nanoparticles and separation rate from the reactants. In order to testify the enhancement of hierarchical diamond-based AuNPs-modified nanocomposites catalytic activity and utility, the catalytic experiments of 4-nitrophenol solution were well accomplished. The prepared nanocomposites exhibited high activity and demonstrated high recyclability without less weight of gold nanoparticles after eight runs of catalysis reduction of 4-nitrophenolm showing potential application in composite catalytic materials.
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