Materials
·
2021
·Vol. 14
·期 11
·3040
·
IF 3.623
Wei Zhang,Kaikai Wang,Xiaodan Hu,Xiaohong Zhang,Shuquan Chang,Haiqian Zhang
A shortage in the supply of3He used for thermal neutron detector makes researchers to find3He alternatives for developing new neutron detectors. Here, we prepared a neutron-sensitive composite liposome with tributyl borate and encapsulating with Fe3O4@oleic acid nanoparticles (Fe3O4@OA NPs), methylene blue (MB), or anti-albumin from bovine serum (anti-BSA). The tributyl borate compound was characterized by Fourier transform infrared spectroscopy (FT-IR). In addition, the morphology, element compositions, and magnetic properties of the composite liposome were investigated with transmission electron microscopy (TEM), energy-dispersive X-ray spectroscopy (EDS), and vibrating sample magnetometer (VSM), respectively. The results indicated that a typical ellipsoidal magnetic liposome structure was obtained, and the lengths of the minor axis and major axis were 49 ± 1 nm and 87 ± 3 nm, respectively. Under thermal neutron irradiation, the structure of composite liposome was destroyed, and encapsulated reporter molecules were released, which was detected by ultraviolet–visible (UV–vis) spectroscopy and surface plasmon resonance (SPR) technology. The response of this sensor based on a destructive assay shows a good correlation with neutron doses. Besides, the sensor has a neutron to gamma-ray rejection ratio of 1568 at a thermal neutron flux rate of 135.6 n/cm2·s, which makes it a promising alternative to3He.Keywords:neutron detector;magnetic liposome;methylene blue (MB);anti-BSA;surface plasmon resonance
研究领域:
核物理
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材料科学
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纳米技术
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辐射检测
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DOI
PubMed
高影响因子
APPLIED BIOCHEMISTRY AND BIOTECHNOLOGY
·
2019
·Vol. 188
·期 3
·769-786
·
IF 2.14
Li Chan,Zhang Wen,Yang Ning,Zhang Qing Song
As one of the typical phyllosilicate clays, hectorite (Hec) has some excellent characteristics and has been greatly applied in adsorption field for the removal of dye, endotoxin, etc. In this study, organic Hec nanocomposites modified with l -Lysine (Lys/Hec NCs) were prepared via solution intercalation method for BR removal. The effects of ionic strength, pH values, initial concentration of BR, and BSA concentration on the adsorption capacity for BR of Lys/Hec NCs were investigated. Results indicated that the adsorption capacity for BR of nanocomposites could reach 40 mg/g when the initial bilirubin concentration was 200 mg/L. However, the adsorption amount of Lys/Hec NCs decreased with increasing the concentration of BSA, but Lys/Hec NCs could still maintain a higher adsorption rate. The adsorption kinetics and adsorption isotherms indicated that the adsorption process of Lys/Hec NCs agreed well with the pseudo-second-order model and the Langmuir isotherm, respectively. Moreover, Lys/Hec NCs also exhibited excellent cytocompatibility. These obtained results demonstrate that Lys/Hec NCs prepared in this study had great potential to be used in hemoperfusion.
研究领域:
生物医学工程
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材料科学
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DOI
PubMed
高影响因子
Scientific Reports
·
2024
·Vol. 14
·期 1
·1-10
·
IF 3.8
Sun Huiwen,Wei Moris,Guo Amber,Zhang Ci,Wang Yuefeng,Huang Renhui,Li Xiaoxiao,Zhan Jeffrey,Wu Jonny,Jiang Bruce
Conjugation to a carrier protein is essential to give rise to the antigenicity of hapten. Three carrier proteins e.g. KLH (Keyhole Limpet hemocyanin), BSA (bovine serum albumin), and OVA (Ovalbumin) were used mostly. KLH is advantageous to the others, majorly owing to its strong immunogenicity and limited usage in other biological assays. However, the cost of obtaining Keyhole Limpet is high and the solubility of KLH is not as well as the other carriers, especially after hapten conjugation. Here, we extracted the shrimp hemocyanin (SHC) from Litopenaeus vannamei (L. vannamei), which is a commonly sea product worldwide. The high pure SHC could be acquired by two-step purification, with a production yield of > 1 g proteins (98% pure) per 1 kg shrimp. Compared to KLH, the peptide-SHC conjugates exhibit higher solubility after hapten conjugation. Meanwhile, compared with KLH, SHC induces comparable antibody production efficiency in mammals, with or without conjugation. Furthermore, rabbit polyclonal antibodies or mouse monoclonal antibodies were generated by immunizing SHC-peptide conjugates, and the subsequent antibodies were confirmed to be used in western blot, immunofluorescence and immunohistochemistry. Therefore, we demonstrated that SHC may be used as a substitute for KLH in future antibody and vaccine development.
研究领域:
免疫学
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蛋白质化学
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生物技术
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DOI
PubMed
高影响因子
JOURNAL OF ENVIRONMENTAL SCIENCE AND HEALTH PART A-TOXIC/HAZARDOUS SUBSTANCES & ENVIRONMENTAL ENGINEERING
·
2022
·
IF 2.582
Hongrui Liu,Yanxuan Ma,Xiang Li,Jiali Gu,Dianbo Dong
Benzophenone (BP)-type UV filters can cause structural changes of carrier protein in plasma. The binding process of five BP-type UV filters with bovine serum albumin (BSA) was investigated by multiple characterization methods, along with their structure-affinity relationship involving the structure of the five BP-type UV filters and their binding affinity for BSA. The BP-type UV filters investigated bound to BSA spontaneously, and altered conformation of BSA. The binding constants and number of binding sites between BP-type UV filters and BSA were 103–106 M−1 and 0.82–1.26, respectively. These BP-type UV filters and BSA interacted with the same binding forces and went through the similar binding process, suggesting that the benzophenone skeleton structure was primarily responsible for the BP-type UV filters and BSA binding, and changes in the structure of the BSA. The BP-type UV filters with hydroxyl substituent (BP-1 and BP-9) and non-polar molecules (BP-6) had a high affinity for binding BSA and had a greater impact on BSA conformation.
研究领域:
化学
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生物化学
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药学
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DOI
高影响因子
RSC Advances
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2021
·Vol. 11
·期 25
·15231-15244
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IF 3.361
Qi Wang,Fengna Dai,Shangying Zhang,Mengxia Wang,Chunhai Chen,Youhai Yu
Zwitterionic nano-silica (SiO2 NPs) obtained by lysine surface modification was used as a hydrophilic inorganic filler for preparing a poly(aryl ether nitrile) (PEN) nanocomposite membrane via an immersion precipitation phase inversion method. The effects of zwitterionic SiO2 NPs addition on the morphology, separation and antifouling performance of the synthesized membranes were investigated. Zwitterionic surface modification effectively avoided the agglomeration of SiO2 NPs. The PEN/zwitterionic SiO2 NPs composite membranes exhibited improved porosity, equilibrium water content, hydrophilicity and permeability due to the introduction of hydrophilic SiO2 NPs in the casting solution, and the optimal pure water flux was up to 507.2 L m−2 h−1, while the BSA rejection ratio was maintained at 97.4%. A static adsorption capacity of 72.9 μg cm−2 and the FRR up to 85.3% in the dynamic antifouling experiment proved that the introduction of zwitterionic SiO2 NPs inhibited irreversible fouling and enhanced the antifouling ability of the PEN membrane.
研究领域:
材料科学
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环境工程
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DOI
PubMed
高影响因子
FOOD RESEARCH INTERNATIONAL
·
2025
·
IF 8
Yujiao Cui,Xiaofei Li,Qingyan Gu,Zhixiang Xu,Longhua Xu
The residual presence of ciprofloxacin (CIP) in aquatic products raises, increasing concerns regarding bioaccumulation and antimicrobial resistance, necessitating the development of highly sensitive and selective detection methods. Here, we present a novel ratiometric fluorescence sensor based on bovine serum albumin-stabilized copper nanoclusters (BSA-CuNCs) and Al 3+ for CIP detection. CIP complexes with Al 3+ to simultaneously quench the orange-red emission of BSA-CuNCs at 600 nm and enhance its intrinsic blue emission at 490 nm, thereby producing a self-calibrated ratiometric signal (F 490 /F 600 ) with a clear concentration-dependent response and an observable color transitions from orange-red to blue. Benefiting from macromolecular surface modification of CuNCs and the ratiometric sensing strategy, the sensor displays good stability, high selectivity, and strong anti-interference capacity. It achieves a wide linear dynamic range of 0.025–50.0 mg L −1 (R 2 = 0.9935), a low limit of detection (0.01 mg L −1 ), and practical potential in complex matrices. Validation in diverse aquatic food samples has yielded satisfactory recoveries of 87.0%–108.8% with RSD < 2.4%. To further expand its practical applicability, a smartphone-assisted sensing platform has been developed, enabling accurate RGB-based quantification (R 2 = 0.9929). Statistical comparison with HPLC using paired t -test showed no significant difference between the two methods ( p > 0.05), indicating high analytical concordance. This work establishes a reliable and versatile approach for accurate quantification and intuitive visual screening of CIP residues, and also suggests the potential for future extension toward simultaneous detection of Al 3+ and CIP in real-world applications.
研究领域:
分析化学
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食品安全
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纳米生物技术
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药物残留检测
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环境监测
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荧光传感
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转化医学
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产品关联
DOI
PubMed
高影响因子
SENSORS AND ACTUATORS B-CHEMICAL
·
2021
·
IF 7.46
Jiayu Jian,Xiao Xiao,Jinxuan Cao,Yizhong Shen
Owing to lack of the effective chromogenic groups, a convenient, precise, and real-time assay for the detection of methyl mercaptan (CH 3 SH) still remains many huge challenges, but it is of great importance to the food and agribusiness related industries. In this work, we proposed an interesting and efficient fluorescence sensing strategy-based alcohol oxidase (AOX)-triggered in situ synthesis of bovine serum albumin-stabilized copper nanoclusters (BSA-CuNCs) for sensitive and selective detection of CH 3 SH. In the presence of CH 3 SH, AOX-assisted O 2 could catalyze it to be H 2 O 2 , which could further trigger BSA-Cu 2+ system to in situ synthesize of BSA-CuNCs, emitting fluorescence signals. On this basis, CH 3 SH concentration-dependent fluorescence emission of BSA-CuNCs was observed, thus allowing for developing a novel fluorescence sensing platform for reporting the levels of CH 3 SH with a broad linear range from 1.0 to 300.0 μM and a low detection limit of 0.19 μM. More significantly, we exploited this sensing strategy to monitor CH 3 SH in real ham samples with acceptable results. This work gives a new insight into applying in situ synthesis strategy to develop a new category of fluorescence sensing platform, achieving convenient, low-cost, reliable, and precise measurements in real food and agriculture-related systems, thereby manifesting a promising potential in food safety monitoring.
研究领域:
分析化学
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食品科学
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纳米技术
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DOI
高影响因子
Chemosensors
·
2026
·Vol. 14
·期 3
·
IF 4.4
Yu-Meng Dai,Weidong Ruan,Hong-Wei Li
This study reports the precise synthesis of red-emitting gold–silver bimetallic nanoclusters (Au-AgNCs) via a one-pot hydrothermal method using thiolactic acid as both the ligand and reducing agent. The Au-AgNCs possess an average diameter of 1.85 nm and exhibit strong fluorescence emission at 687 nm. Furthermore, they display notable assembly-induced emission enhancement (AIEE) properties. Upon assembly with bovine serum albumin (BSA), their fluorescence quantum yield significantly increases from 2.50% to 7.78%. Then Au-AgNCs@BSA assembly was employed as a ratiometric fluorescence sensor for the detection of chlortetracycline (CTC). In the presence of CTC, the original red emission of the assembly at 687 nm remained stable, while a new blue emission emerged at 420 nm and intensified progressively with CTC concentration. The ratio of the two emission intensities (I420/I687) exhibited an excellent linear correlation with CTC concentration over the range of 0.10 to 15 μM, with a limit of detection (LOD) of 20 nM. Notably, the sensor demonstrated exceptional selectivity for CTC, showing negligible response to common interfering substances such as metal ions, anions, amino acids, and crucially, other tetracycline antibiotics (tetracycline, oxytetracycline, and doxycycline). The practical applicability of the sensor was validated through the determination of spiked CTC in real water samples, achieving satisfactory recovery rates. In conclusion, this work accomplishes two key objectives: the development of novel AIEE-active Au-Ag bimetallic nanoclusters and the design of an efficient ratiometric sensing strategy. This approach enables the highly selective and sensitive detection of CTC, offering a promising tool for environmental monitoring.
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高影响因子
INTERNATIONAL JOURNAL OF BIOLOGICAL MACROMOLECULES
·
2025
·
IF 8.5
Jiaojiao Su,Yaning Zhang,Mingli Yue,Jian Liu
Cell chirality is a complex phenomenon that imparts unique properties. The creation of biomimetic artificial cells chirality is a rapidly evolving research topic that has had a profound impact in multiple fields, including materials science, catalysis, biotechnology, and biomedicine. Herein, we report a simple method that combines L- or D-penicillamine (L/D-Pen) with proteinosomes on the surface of gold nanoparticles to afford nanoparticle hybrid microcapsules. Encapsulation of the enzyme in the microcapsule enables a chiral recognition effect on the membrane surface, thereby affording control over the enantioselective catalytic reaction of the d -glucose (D-Glu) substrate loaded inside the hybrid microcapsule. The adsorption energy of D-Glu and D-Pen (−0.095) calculated by MS is less than that of D-Glu and L-Pen (−0.064). Thus enantiomeric selective recognition is a stronger interaction force than adsorption for the same configuration. These findings highlight the development of biomimetic chiral microcompartments, and provide a platform for driving cellular chirality in various organisms.
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DOI
PubMed
高影响因子
Frontiers in Molecular Biosciences
·
2020
·
IF 4.188
Han Xiao,Xu Xiaoquan,Tang Yuxia,Zhu Feipeng,Tian Ying,Liu Wei,He Doudou,Lu Guangming,Gu Yunfei,Wang Shouju
Anaplastic thyroid cancer (ATC) is a highly aggressive and the most lethal type of thyroid cancer. The standard-of-care for unresectable ATC is radiotherapy and chemotherapy, usually based on doxorubicin (Dox). However, most patients develop resistance shortly after treatment. To overcome the drug resistance, we synthesized the mesoporous organosilica nanoparticles (MONPs) loaded with Dox and stabilized the nanocomposites by bovine serum albumin (BSA). The surface area and pore volume of MONPs were 612.653 m2/g and 0.589 cm3/g. The loading capacity of Dox-MONPs reached 47.02%. Compared to Dox-MONPs and free Dox, BSA-Dox-MONPs had more durable tumor-killing power on both drug-sensitive cell line HTh74 and drug-resistant cell line HTh74R. The cellular uptake of BSA-Dox-MONPs was 28.14 and 65.53% higher than that of Dox-MONP in HTh74 and HTh74R. Furthermore, the BSA coating decreased the efflux rate of nanocomposites in HTh74 (from 38.95 to 33.05%) and HTh74R (from 43.03 to 32.07%). In summary, BSA-Dox-MONPs reversed the chemotherapy resistance of ATC cells via increased drug uptake and inhibited drug efflux, offering a promising platform for the treatment of chemo-resistant ATC.
研究领域:
肿瘤学
·
纳米技术
·
药学
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产品关联
DOI
PubMed
高影响因子
Theranostics
·
2020
·
IF 8.579
Qingqing Tian,Lu An,Qiwei Tian,Jiaomin Lin,Shiping Yang
Rationale : Chemodynamic therapy (CDT) based on the Fe(II)-mediated Fenton reaction is an emerging tumor treatment strategy. However, the catalytic efficiency in tumors is crucially limited by Fe(II). Herein, an endogenous hydrogen sulfide (H 2 S) accelerated Fe(III)/Fe(II) transformation and photothermal synergistically enhanced CDT strategy based on ellagic acid-Fe-bovine serum albumin (EA-Fe@BSA) nanoparticles (NPs) was developed for colon tumor inhibition. On the one hand, the Fe(III) with low catalytic activity in the EA-Fe@BSA NPs could be rapidly reduced to the highly active Fe(II) by the abundant H 2 S in colon cancer tissues. Thus, a rapid Fe(III)/Fe(II) conversion system was established, wherein highly active Fe(II) ions were continuously regenerated to improve the CDT efficiency. On the other hand, the photothermal effect of EA-Fe@BSA NPs also accelerated the production of hydroxyl radicals (•OH), thereby synergistically enhancing the CDT performance and improving the therapeutic efficacy. Methods : The endogenous H 2 S accelerated Fe(III)/Fe(II) conversion and PTT enhanced CDT were investigated by characterization of the Fe valence state and detection of •OH. T 1 -weighted magnetic resonance imaging (MRI) was tested both in vitro and in vivo . The biocompatibility of NPs were examined via MTT assay, hemolysis analysis and routine blood measurements. The enhanced CDT was investigated in HCT116 colon cancer cells by Calcein-AM/PI staining and MTT assay, and tumor inhibition was demonstrated in HCT116 tumor bearing mice. Results : In this work, EA-Fe@BSA NPs were constructed as a CDT theranostic reagent. The H 2 S accelerated Fe(III)/Fe(II) conversion was confirmed, more degradation of MB and generation of •OH demonstrated the enhanced CDT in vitro . EA-Fe@BSA NPs exhibited good T 1 -weighted MRI performance. More importantly, it displayed strong near-infrared (NIR) absorption and excellent photothermal efficiency, further promotes the production of •OH. Hence, the efficacy of CDT was enhanced, and the tumor growth was inhibited efficiently. Conclusion : All results demonstrate that this strategy based on endogenous H 2 S promoted Fe(III)/Fe(II) transformation together with PTT acceleration permits efficient Fenton-reaction- mediated CDT both in vitro and in vivo , which holds great potential for effective colon cancer theranostics.
研究领域:
生物医学工程
·
肿瘤治疗
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纳米医学
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产品关联
DOI
PubMed
高影响因子
DYES AND PIGMENTS
·
2011
·
IF 2.635
Shenguang Ge,Juanjuan Lu,Mei Yan,Feng Yu,Jinghua Yu,Xiujin Sun
Bovine serum albumin in micelles was effectively detected by a fluorescence resonance energy transfer -based molecular sensor using CdTe quantum dots as energy donors and neutral red as energy acceptors. The fluorescence resonance energy transfer efficiency was enhanced by the construction of cetyltrimethylammonium bromide micelles, which significantly reduced the distance between the donor and the acceptor. The results indicated that the addition of the albumin easily leads to the fluorescence quenching of CdTe-neutral red fluorescence resonance energy transfer due to the formation of CdTe-albumin complexes which have weak affinity to CdTe and repels the dyes from CdTe surface. The fluorescence intensity of the CdTe-neutral red sensor quenched with the increasing concentration of albumin. The linear range of albumin was from 0.4 × 10 −3 to 11.00 × 10 −3 g L −1 . The detection limit was 0.13 g L −1 .
研究领域:
生物化学
·
分析化学
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产品关联
DOI
高影响因子
危险品化学品经营许可证（带存储）