查找使用阿拉丁产品的科研文献

A Western dietary pattern is related to higher risk of allergic rhinitis in young women

H. Rezapour,M. Fereidouni,Z. Asadi,M. Saharkhiz,M. Ayadilord,S. Mahmoudzadeh,M.R. Shadmand Foumani Moghadam,G.A. Ferns,A. Bahrami

The prevalence of allergic disorders has been increasing globally, and it may be useful to identify the modifiable factors associated with it. Diet has been identified as one possible lifestyle factor implicated in the pathogenesis of allergic diseases. The goal of this study was to evaluate the associations between dietary patterns and the presence of allergies in a group of young women in eastern Iran. In all, 181 female students were enrolled. Screening for presence of allergic diseases, including allergic rhinitis (AR), asthma and eczema, was performed by an expert allergist. Information about dietary intake was collected using a validated 65-item food frequency questionnaire. Exploratory factor analysis was used to evaluate the relationships between food/nutrition variables. Serum immunoglobulin (Ig)E levels were significantly higher in the group in the third tertile (highest adherence) for a Western dietary pattern compared to the group in the first tertile (lowest adherence). The multivariate odds ratio for presence of allergic rhinitis (AR) was 2.5 (95% CI:1.1-5.1) for the highest compared to lowest tertile of the Western dietary pattern score. However, no significant relationship was found between a traditional dietary pattern and AR, asthma or eczema. Our findings indicate a potential role in the development of AR of the Western dietary pattern, which is characterized by high intake of dairy products, snacks, nuts and sugar. Since diet is a modifiable factor, the relationship between AR and a Western dietary pattern may have clinical implications, particularly in subjects with other risk factors for developing AR. Résumé La prévalence des troubles allergiques a augmenté à l’échelle mondiale, et il peut être utile d’identifier les facteurs modifiables qui y sont associés. L’alimentation a été identifiée comme un facteur de style de vie possible impliqué dans la pathogenèse des maladies allergiques. Le but de cette étude était d’évaluer les associations entre les habitudes alimentaires et la présence d’allergies dans un groupe de jeunes femmes de l’est de l’Iran. Au total, 181 étudiantes se sont inscrites. La présence de maladies allergiques dont la rhinite allergique (RA), l’asthme et l’eczéma a été identifiée par un allergologue expert. Les informations sur l’apport alimentaire ont été recueillies à l’aide d’un questionnaire de fréquence alimentaire validé de 65 éléments. Une analyse factorielle exploratoire a été utilisée pour évaluer les relations entre les variables alimentaires/nutritionnelles. Les taux sériques d’immunoglobulines (Ig) E étaient significativement plus élevés dans le groupe du troisième tertile (adhérence la plus élevée) pour un régime alimentaire occidental par rapport au groupe du premier tertile (adhérence la plus faible). L’odds ratio multivarié d’avoir une rhinite allergique (RA) était de 2,5 fois (IC à 95 % : 1,1 à 5,1) pour le tertile le plus élevé par rapport au tertile le plus bas du score de modèle alimentaire occidental. Mais, aucune relation significative n’a été trouvée entre un régime alimentaire traditionnel et la RA, l’asthme ou l’eczéma. Nos résultats indiquent un rôle potentiel du modèle alimentaire occidental, caractérisé par sa teneur élevée en produits laitiers, en collation, en noix et en sucre, dans le développement de la RA. Étant donné que le régime alimentaire est un facteur modifiable, la relation entre la RA et un modèle alimentaire occidental peut avoir des implications cliniques, en particulier chez ceux qui ont d’autres facteurs de risque de développer une RA.

Chemical Compositions and Oxidative Stabilities of Ginkgo biloba Kernel Oils from Four Cultivated Regions in China

Liangliang Xie,Tao Zhang,Liyou Zheng,Dan Xie,Jun Jin,Xingguo Wang,Qingzhe Jin

Ginkgo biloba is a living fossil and traditionally known as a source of pharmaceuticals and cosmetics, largely because of the lipids in the kernels. In the present study, fatty acids, triacylglycerols, micronutrients, and oxidative stabilities of G. biloba kernel oils from four major different cultivated regions in China were systematically analyzed. The results showed that the kernel oils contained more than 26 triacylglycerol species with high contents of linoleic acid (42.04–43.01%), oleic acid (14.33–18.00%), and trans- vaccenic acid (15.13–16.86%). Functional fatty acids such as trans- vaccenic acid, palmitoleic acid, and podocarpic acid were particularly enriched in the oils. In addition, high levels of tocopherols (173.41–272.18 mg/100 g) and 304.09–673.26 mg/100 g of phytosterols in the oils contributed to significantly improve their oxidative stabilities. In particular, α-tocopherol (106.63–127.35 mg/100 g), γ-tocopherol (41.41–133.65 mg/100 g), campesterol (53.22–139.88 mg/100 g), and β-sitosterol (245.50–522.62 mg/100 g) were the dominant micronutrients; while γ-tocopherol significantly affected oxidative stabilities of the four oils from different regions. It is suggested that the G. biloba kernel oils are ideal sources for high-valued usages in skin care purpose, physical therapy, functional food, and nutritional supplements.

Microbe-imprinted polymers for rapid drug-resistant bacteria recognition

Yuanzi Wu,Kaiqiang Zhou,Wenhui Li,Min Huan,Zhichao Yu,Fen Yan,Huixiao Hong,Dianping Tang,Yansong Guo,Zuquan Weng

The rise of antibiotic-resistant bacteria poses a serious global health threat, highlighting the urgent need for novel strategies beyond conventional antibiotic therapies. This study explores the potential of microbe-imprinted polymers (MIPs) as innovative, pathogen-specific affinity agents. Utilizing microbial surface-initiated polymerization, MIPs are in-situ synthesized on the surface of target microbes, creating flexible heteropolymers that precisely replicate microbial surface structures. This method exhibits high affinity ( K D = 2.7×10 8 CFU/mL for E. coli ) and selectivity at the strain level. MIPs offer significant advantages over traditional antibodies, including greater stability, cost-effectiveness, and a broader spectrum of binding capabilities, making them effective for identifying and targeting various microbial strains, including unidentified or drug-resistant variants. Moreover, their favorable biocompatibility and functional resilience in diverse environments position MIPs as promising candidates for rapid pathogen detection and therapeutic applications. This research paves the way for advanced biomimetic materials in microbe-specific diagnostics and combating infections, addressing the critical need for effective tools in antibiotic resistance surveillance.

Hyaluronic-acid-based β-cyclodextrin grafted copolymers as biocompatible supramolecular hosts to enhance the water solubility of tocopherol

Parbeen Singh,Li Wu,Xiaohong Ren,Wei Zhang,Yan Tang,Yongli Chen,Andrew Carrier,Xu Zhang,Jiwen Zhang

Hyaluronic acid (HA), a common biopolymer found in the extracellular fluid, was grafted with β-cyclodextrin (β-CD) to form a composite polymer that could form inclusion complexes with tocopherol (VE), enhancing its water-solubility and serving as a model drug delivery system . Herein, different copolymers were prepared with varying HA:β-CD ratios and characterized. VE loading capacity was directly correlated with increased β-CD composition in the polymers and morphological changes were observed upon VE binding. The host materials and their VE inclusion complexes are not cytotoxic, and are thus useful for VE and drug delivery.

Robust Superhydrophobic E-Textiles Based on a Low-Wetting Conductive Interface for Health Management

Peng Jun,Jia Jiru,Tang Jinglei,Tang Jicheng,Wang Chaoxia

Functional e-textiles have garnered increasing attention in both academic and commercial domains. However, the development of e-textiles that simultaneously exhibit efficient electrothermal physiotherapy, effective electromagnetic interference (EMI) shielding, and reliable sensing capabilities remains a significant challenge. In this study, a robust superhydrophobic e-textile is developed through the fabrication of a three-dimensional interlocked conductive coating comprising MWNTs, MXene, and AgNWs, along with a low-wettability surface interface. The low-wettability interface imparts pronounced superhydrophobicity, for which the water contact angle is 155.2°, effectively protecting the conductive components from exfoliation and chemical damage. The integration of the MWNTs/MXene/AgNWs conductive coating with the prestretching process endows the superhydrophobic e-textile with exceptional strain-sensing performance, characterized by high sensitivity (414.8), rapid response time (40 ms), and a low detection threshold (0.1%). Furthermore, the formation of the interlocked conductive coating confers excellent electrical conductivity of up to 1826 S·m–1, enabling remarkable EMI shielding effectiveness (45.4 dB) and electrothermal performance (102.3 °C at 4.0 V). These results indicate that the developed superhydrophobic e-textile holds significant potential for utilization in fields such as EMI shielding, electrothermal physiotherapy, and motion tracking.

Gold-Polyaniline Supercapacitive Biosensor for Selective Detection of E. Coli DH 5α

Md. Abdul Khaleque,Md. Hasibul Hassan,Md. Romzan Ali,Md. Anwar Hossain,Mohamed Aly Saad Aly,Waleed Alahmad,Munawar Sultana,Md. Zaved H. Khan

Bacterial identification is challenging due to several problems, such as low sensitivity, poor selectivity, and the lack of quantitative methods. To address these obstacles, a gold nanoparticles (AuNPs)@Polyaniline (PANI)/Phage electrode was developed. The bacteriophage was immobilized on the AuNPs@PANI modified electrode, serving as an electrochemical biosensor of Escherichia coli (E. coli) DH5 $\alpha$ . In application, this biosensor can detect of E. coli DH5 $\alpha$ within a dynamic range of 10–107 CFUmL-1 with the detection limit of 1.0 CFUmL-1. Modified AuNPs@ PANI/Phage electrode exhibits a signal/noise ratio equal to 3.0, but only AuNPs@ PANI displayed 1.025 in [Fe(CN)6]3−/4− This biosensor selectively detected of E. coli DH5 $\alpha$ bacteria in the presence of comparable concentrations of different bacterial strains. In addition, the modified electrode exhibited supercapacitive behavior during electrochemical characterization and showed a high specific capacitance of 782.61 Fg-1 at 1.0 Ag-1 and 94.7% retention over 10 000 GCD cycles. Further analysis was conducted using galvanostatic charge-discharge to measure capacitance and self-discharge to assess capacity retention over time, and the Dunn method to analyze the charge storage mechanism within the electrode material. In conclusion, the developed nanostructure displayed dual electrochemical behavior as a supercapacitor and biosensor. This is the first reported PANI functionalized capacitive-controlled electrodes for bacterial detection. The fabrication and detection process for a single glass carbon electrode (GCE)/AuNPs@PANI pernigraniline salt (PANI-PS)/Phage/E. coli electrode requires ~90 min, with an estimated material cost of U.S. ${\$}$ 5.28 per electrode. Furthermore, smartphone-based testing confirmed portable, realtime detectionwith <1.5% deviation, indicating promise for multichannel clinical biosensing.

E-Synapse Based on Lead-Free Organic Halide Perovskite (CH3NH3)3Sb2Cl9 for Neuromorphic Computing

Ziquan Ni,Yangbin Zhu,Songman Ju,Zhongwei Xu,Fengqing Tian,Hailong Hu,Tailiang Guo,Fushan Li

Roles of Indole and Its Derivative in Modulating E. coli–Candida albicans Biofilm Formation

You-Quan Ma,Lan Lin

Candida albicansis the causal agent of invasive candidiasis, which might be lethal in immunocompromised patients. Biofilm formation is considered a key virulence factor ofC. albicansand is associated with its elevated resistance to antifungals.C. albicansand bacteria likeE. coliare frequently found to form mixed biofilms on biotic or abiotic surfaces, rendering them more refractory to existing antifungals. To investigate howE. coliendogenous indole interplaying with exogenous IAA exerts modulatory effects on dual-species biofilm withC. albicans, anE. colistrain deficient in the indole biosynthetic genetnaAwas constructed, and the enzyme TnaA inhibitor was administered to block the indole production inE. colimonoculture and/orE. coli–C. albicansdual culture. Phenotypic assay revealed that indole deficiency attenuatedE. colimono-species biofilm by 12% (tnaA∆ versus WTE. coli), and the lack of indole in theE. colicell-free culture filtrate abolished the ability to promoteC. albicansbiofilms, evidenced by the fact that the treatment with WTE. coliculture supernatants exhibited a 1.7-fold promotive effect, while treatment withtnaA∆ displayed no significant difference from the broth control towardsC. albicansbiofilms. Furthermore, impairedE. coliindole production might disruptE. coli–C. albicansbiofilm, as examined by confocal laser scanning microscopy (CLSM). Moreover, indole-3-acetic acid (IAA) was found to exhibit more potent biofilm-modulatory activity than indole by CLSM imaging with dual biofilms of WTE. coli–C. albicans, in contrast to those ofE. coli tnaA∆–C. albicanspost-supplemented with exogenous IAA. This study provides evidence for indole as a signaling molecule mediating bacterial–fungal communication during mixed-biofilm formation. Indole and its derivatives, particularly in combination with existing antifungals, have potential in the development of anti-biofilm strategies to eradicate refractory fungal infections.

Extraction, Characterization, and Immunological Adjuvant Effect Evaluation of Δlpxl E. coli Outer Membrane Vesicles

Zhu Mingxue,Wei Lin,Han Xiangning,Li Guoqiang,Cao Limin,Wang Xiudan,Wang Kaiqiang,Lin Hong,Sui Jianxin

The preparation of specific antibodies is of critical importance in the immunoassay of small-molecule contaminants in food. However, due to the lack of immunogenicity, these small molecules must be conjugated to a carrier to form complete antigen in order to induce the generation of antibodies, and adjuvants are typically required during the immunization when traditional proteins were used as carriers. Therefore, identifying a substance that possesses both the characteristics of carrier and adjuvant is of significant research importance for antibody preparation. Outer membrane vesicles (OMVs) are nanoscale particles with a bilayer membrane and spherical structure produced by Gram-negative bacteria through natural budding. They contain various pathogen-associated molecular patterns (PAMPs), which could be recognized by pattern recognition receptors (PRRs) on the surface of immune cells, thereby triggering a robust immune response. In this study, OMVs derived from Δ lpxl E. coli and chloramphenicol sodium succinate (CSS) were used as the carrier and hapten to synthesize the complete chloramphenicol (CAP) antigen (CSS-Δ lpxl -OMV). BALB/c mice were subsequently immunized with this antigen to generate antibodies, with the aim of evaluating the adjuvant effects of Δ lpxl -OMV and its potential as a hapten carrier. The results indicated that Δ lpxl -OMV not only served as a carrier for binding the hapten to prepare the complete antigen but also exhibited significant adjuvant properties. The synthesized complete antigen possessed significant immunogenicity and could induce the mice to produce antibodies against the target without additional adjuvants.

Rapid Detection of Dextromethorphan in Saliva and E-Juice Based on Highly Selective Aptamers

He Yijing,Yang Yue,Wang Huixi,Zhang Fan,Cai Wentao,Yu Haixiang,Di Bin

Dextromethorphan (DXM) is a widely used cough suppressant, and its escalating abuse is a pressing global health concern that necessitates rapid screening tools. However, rapid methods for detecting DXM in complex matrices remain highly limited. Here, we report the first isolation and engineering of DNA aptamers specifically targeting DXM. Using library-immobilized SELEX, we identified high-affinity aptamers ( K D ∼ 1–2 μM) with exceptional specificity against various interferents. The lead candidate was rationally truncated into a minimal 27-nucleotide core (DM1–27) that retains full function. Systematic mutagenesis and molecular modeling further delineated the structure–activity relationship governing target recognition. Utilizing this engineered aptamer, we developed two distinct fluorescent platforms: a label-free thioflavin T displacement assay and a fluorophore-quencher labeled hybrid beacon. Both sensors enabled robust DXM quantification within 10 min in human saliva and e-cigarette juice (EJ), achieving limits of detection at low ng/mL-level. Notably, the hybrid beacon significantly improved matrix tolerance by bypassing short-wavelength background interference. This work provides a versatile molecular recognition tool and establishes practical, field-deployable sensing strategies for DXM monitoring.

Study on dexamethasone-fast dissolving oral film based on pullulan-vitamin E succinic acid

Lan Tang,Xin Zhang,Ye Huang,Depeng Kong,Yiwen Yan,Zhimin Ou,Mulan Zhu,Yi Li,Jianwei Wang

Objective This study aimed to enhance the solubility and dispersion of poorly water-soluble dexamethasone (DEX) in oral films by developing a novel pullulan-vitamin E succinate (PUL-VES) coupling compound (designated PUVS3) as the film matrix. Methods The amphiphilic coupling compound PUVS3 was synthesized, characterized and evaluated. Using PUVS3 as a solubilizing/suspension agent, and PUL as the film-forming material, a dexamethasone-fast dissolving oral film (DEX-FDOF) was prepared by solvent casting method. The film was evaluated for appearance, microstructure, mechanical properties, weight variation, content uniformity, disintegration time and in vitro dissolution. Results The DEX-FDOF formulation exhibited a smooth surface with no observable drug precipitation and demonstrated excellent transparency. Both weight variation and content uniformity complied with the pharmacopoeial standards. The film displayed rapid disintegration (<15s) and favorable mechanical properties with an elastic modulus of 35–45 MPa and elongation at break of 17%–20%. Notably, in vitro dissolution reached approximately 47 % within 10 min, representing a significant improvement over the pure drug. This enhancement was achieved through the use of a self-synthesized PUL-VES coupling compound as the film matrix. This innovative approach enabled homogeneous dispersion of the poorly water-soluble DEX within the water-soluble oral film while simultaneously improving its solubility characteristics. Conclusion The DEX-FDOF, fabricated using the self-synthesized PUL-VES coupling compound as the matrix, exhibited excellent uniformity, mechanical strength, rapid disintegration (<15 s), and notably enhanced dissolution (47 % in 10 min), demonstrating its potential as an effective delivery system for poorly soluble drugs such as DEX.

Targeting threonine deaminase with chiral Au NPs: A novel strategy for E. coli inhibition

He Wang,Haidu Yuan,Jianhao Zhang,Wenjing Yan

Bacterial infections are becoming a significant threat to global human health due to the growing prevalence of biofilm-related infections and the rise in antibiotic resistance. D/ l -cysteine functionalized chiral gold nanoparticles (D/P-Au NPs or L/P-Au NPs) have demonstrated a potent antibacterial effect against E. coli , while the mechanism remains to be elucidated through additional research. Threonine deaminase (TD) is a crucial enzyme involved in branched-chain amino acid (BCAA) biosynthesis in E. coli and is involved in cysteine's antimicrobial effects. This study investigated the interaction between chiral Au NPs (D/P-Au NPs or L/P-Au NPs) and TD as well as its effect on enzyme activity. It demonstrates that chiral Au NPs interact with TD through hydrophobic forces, forming a ground state complex that induces changes in the secondary structure of TD and reduces enzyme activity in a concentration-dependent manner. We found that the exogenous supplementation of isoleucine and valine (2 mg/mL) significantly reduced the antibacterial activity of chiral Au NPs, especially for L/P-Au NPs. The proteomics results indicate that the expression of ilvA and ilvB was down-regulated after L/P-Au NPs treatment, which would interfere with the synthesis of BCAAs. These results demonstrate that chiral Au NPs cause cell death of E. coli partly due to inhibition of TD enzyme activity and the synthesis of branched-chain amino acids.