Innovative Food Science & Emerging Technologies
·
2019
·
IF 4.085
Yao Xiong,Qianru Li,Song Miao,Yi Zhang,Baodong Zheng,Longtao Zhang
To investigate the effects ultrasound (20 kHz, 150–600 W) on physicochemical properties of emulsion stabilized by myofibrillar protein (MP) and xanthan gum (XG), the emulsions were characterized by Fourier transform infrared (FT-IR) spectroscopy, ζ-potential, particle size, rheology , surface tension, and confocal laser scanning microscopy (CLSM). FT-IR spectra confirmed the complexation of MP and XG, and ultrasound did not change the functional groups in the complexes. The emulsion treated at 300 W showed the best stability, with the lowest particle size, the lowest surface tension (26.7 mNm −1 ) and the largest ζ-potential absolute value (25.4 mV), that were confirmed in the CLSM photos. Ultrasound reduced the apparent viscosity of the MP-XG emulsions, and the changes of particle size were manifested in flow properties. Generally, ultrasound was successfully applied to improve the physical stability of MP-XG emulsion, which could be used as a novel delivery system for functional material.
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高影响因子
Frontiers in Immunology
·
2019
·
IF 4.716
Wu Liting,Kong Linghe,Yang Yanjian,Bian Xia,Wu Siwei,Li Bingxi,Yin Xiaoxue,Mu Liangliang,Li Jun,Ye Jianmin
Teleost B cells have phagocytic activities for ingesting particulate antigens, such as bacteria, in addition to the functional secretion of immunoglobulins (Igs). In the present study, the phagocytic activities of IgM+ B cells under various differentiational conditions residing in peripheral blood leukocytes were investigated in a teleost fish Nile tilapia (Oreochromis niloticus). The IgM+ B cells were recognized as IgMlo or IgMhi subsets based on their membrane IgM (mIgM) levels. The mIgM, secreted IgM (sIgM), major histocompatibility complex class II and reactive oxygen species were detected. Expressions of transcription factors (Pax5 and Blimp-1) and B cell signaling molecules (CD79a, CD79b, BLNK, and LYN) suggested that IgMlo B cells were resembling as plasma-like cells and IgMhi resembling as naïve/mature B cells, respectively. Analysis of phagocytic activities demonstrated that both IgMlo and IgMhi B cells have a similar phagocytic ability (phagocytosis percentage); however, the phagocytic capacity [phagocytic index and the mean fluorescence intensity (MFI)] of IgMhi B cells was significantly higher than that of IgMlo B cells. Taken together, the results indicated that B cell differentiation may cause the decrease of phagocytic capacity but not phagocytic ability of phagocytic IgM+ B cells in teleost. The finding may provide an evolutionary evidence for understanding the greater specialization of the B cell in more sophisticated adaptive humoral immunity, by decreasing phagocytic activity in order to contribute its function more specifically into antibody-secreting.
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PubMed
高影响因子
Micro & Nano Letters
·
2018
·Vol. 13
·期 6
·868-871
·
IF 0.841
Yan-meng Li,Li-wei Wang,Ruo-ping Li,Jun-he Han,Ming-ju Huang
Malachite green (C 23 H 25 ClN 2 ) is an illegal fungicide of aquatic products due to its potential carcinogenic, teratogenic, and mutagenic effects. It is typically challenging to detect C 23 H 25 ClN 2 in fish with conventional techniques. The study reported a simple and rapid method for detecting C 23 H 25 ClN 2 on fish surface with surface-enhanced Raman scattering (SERS). Using the seed growth method with trometamol as the modifier, 110 nm in-diameter gold nanoparticles on thin glass as SERS substrate were prepared, which exhibited high SERS activity, reproducibility, stability, and flexibility in SERS measurement. The fish samples required no pretreatment and the entire measurement took <5 min. Quantitative analyses were also conducted. Excellent linear relationships ( R 2 = 0.9397–0.9977) were obtained between the concentrations and the peak intensities of C 23 H 25 ClN 2 at some characteristic peaks with a wide concentrations range (0.5–10 μM). The detection limit can reach 0.5 μM. This method provided an easy and fast way to detect C 23 H 25 ClN 2 on fish surface for food safety.
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高影响因子
ECOTOXICOLOGY AND ENVIRONMENTAL SAFETY
·
2018
·
IF 3.974
Suiliang Huang,Wenwen Kong,Zhenjiang Yang,Hui Yu,Fengyuan Li
The ecological health of aquaculture water is threatened by wasted fish feed and herbicides. In order to study the effect of prometryn and fish feed on Microcystis aeruginosa growth based on Monod and Logistic functions, four different concentrations of prometryn (0, 50, 100 and 200 μg L −1 ) and two different dosages of fish feed (0.075 g, 0.15 g; d < 0.85 mm) were added into the culture medium, and the fish feed was the source of nitrogen and phosphorus in the MII medium. Results showed that Microcystis aeruginosa growth can be fitted well by Logistic and modified Logistic functions with 0–200 μg L −1 prometryn ( R 2 = 0.981–0.998 and R 2 = 0.989–0.999, respectively). With the same concentration of prometryn, the maximum algae density ( N max ) of Microcystis aeruginosa calculated by both Logistic and modified Logistic functions increased with increasing dosage of fish feed and with the same dosage of fish feed, N max declined with increasing concentrations of prometryn. Inhibition of prometryn on algae growth stimulated by fish feed is of double concentration-dependence, inhibition rates ( I ) are lower in 0.15 g fish feed medium than 0.75 g ones generally, implying that more nutrients can alleviate the stress caused by prometryn on algae. Derived formula for the specific growth rate, growth rate and inhibition rate using modified Logistic function agreed reasonably well with measured data. Jointly application of modified Monod and Logistic functions can better describe the relationship between specific growth rates and nutrients concentrations compared to combination of Monod and Logistic functions. In addition, equations for describing variations of nutrients concentrations (PO 4 3- -P and NH 4 + -N) with time were also derived based on both modified Monod and Logistic functions, which agree reasonably well with the measured data. In sum, the combination of modified Monod and Logistic functions provides a promising and robust method in studying algal growth stimulated by fish feed in incubator experiments.
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高影响因子
CHROMATOGRAPHIA
·
2018
·Vol. 81
·期 6
·881-889
·
IF 1.401
Li Shan,Yang Zhaoguang,Cao Junfei,Qiu Bo,Li Haipu
Abstract The content of metallothionein (MT) in fish can serve as a biomarker for heavy metal contamination in an aquatic environment. In this paper, an improved sample separation and quantification method using cadmium saturation combined with anion exchange high performance liquid chromatography–inductively coupled plasma mass spectrometry (HPLC–ICP-MS) was developed to measure the two major isoforms of MT (MT-I and MT-II) in fish. Replacing sulfur with cadmium as the quantitative element by cadmium saturation reduced the instrumental requirement and analysis cost. In the chromatographic separation, NH 3 –NH 4 Cl buffer was applied as the mobile phase instead of Tris–HCl buffer to prevent carbon deposition on the ICP cone. The chloride concentration was found to be a key factor to the separation of MT-I and MT-II, and the optimum chromatographic condition was 27 mM of NH 3 –NH 4 Cl in isocratic mode, with a pH of 8.6 and a flow rate of 0.8 mL min −1 . The limits of detection were 0.7 μg g −1 for MT-I and 1.0 μg g −1 for MT-II, with recoveries ranging from 96.2 to 104.2% for MT-I and 84.7 to 90.4% for MT-II in different fish tissues. The relative standard deviations were less than 8% for both isoforms. Graphical abstract
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高影响因子
Food Analytical Methods
·
2018
·Vol. 11
·期 6
·1668-1676
·
IF 2.245
Jia Jie,Yan Shang,Lai Xiaoxia,Xu Yongzhen,Liu Tong,Xiang Yuhong
A facile colorimetric aptasensor based on unmodified gold nanoparticles (AuNPs) for rapid, highly sensitive and selective detection of malachite green (MG), one of triphenylmethane dyes and antibiotics, was successfully developed. Malachite green RNA aptamer having the binding ability with high affinity and specificity would be the ideal recognition receptor for MG. RNA aptamer can adsorb on the AuNPs and protect AuNPs against salt-induced aggregation. When malachite green were added in a solution, aggregation of AuNPs was specifically induced by desorption of aptamer from the surface of gold nanoparticles as the formation of aptamer-target complex. Therefore, the salt could trigger AuNP aggregate and the solution color changed from red to blue. The solution colors and absorbance were related with the concentrations of MG, and a well linear relationship in the wide range was obtained. The detection limit of MG was down to 15.95 nM. This colorimetric method was better than other traditional methods in terms of its reduced detection time, simple signal generation, high selectivity, lower detection limit, and observation with the naked eye. The proposed one-step, label-free method was successfully used to determine MG in actual fish samples. The high recoveries of MG in fish sample detection were satisfied.
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高影响因子
Fishes
·
2025
·Vol. 10
·期 3
·92
·
IF 2.4
Yijun Jia,Zhao Zhao,Leyang Chen,Yongqi Liu,Bin Zhu
A number of viral diseases have significantly impeded the growth of the aquaculture industry. Antiviral drugs represent an effective means of controlling infection. However, the efficacy of the entire therapeutic process is contingent upon the availability of an efficient delivery system. This study selected three common antiviral drugs and constructed corresponding drug delivery systems utilising single-walled carbon nanotubes (SWCNTs) as carriers. The reliability of carbon nanotubes as delivery carriers was evaluated by detecting the therapeutic effect on infectious splenic and renal necrosis virus (ISKNV). The findings demonstrated that SWCNTs can effectively enhance the absorption of the three drugs into the body, prolong their metabolic half-life, and improve the survival rate of fish infected with ISKNV. The Ribavirin-SWCNTs (RBV-SWCNTs) group exhibited the most pronounced protective effect, with a mortality rate of less than 25%. It was observed that SWCNTs facilitated the rapid transportation of ribavirin, with the drug content in the RBV-SWCNTs group being approximately double that of the free ribavirin group. Furthermore, this system markedly diminished the viral load, augmented enzyme activities, and elevated antiviral gene expression. This study indicated that carbon nanotubes are optimal carriers for antiviral drugs, which have considerable potential as a delivery vehicle for antiviral drugs to prevent viral infections in aquaculture.
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高影响因子
LWT-FOOD SCIENCE AND TECHNOLOGY
·
2024
·
IF 6
Xiao-Chen Huang,Hao Chen,Shou-Lian Wei,Jin-Kui Ma
Sulfonamide antibiotics with superior performance are widely used in aquaculture. However, the residues of sulfonamide antibiotics in aquatic products may pose a threat to human health. In this work, a novel type of magnetic molecularly imprinted polymer (mMIP) was synthesized and coupled with ultra-high performance liquid chromatography–tandem mass spectrometry (UHPLC-MS/MS) for the selective separation and determination of 4 sulfonamide antibiotics residues in tilapia. The structure and adsorption properties of the polymers were characterized and evaluated. The key mMIP variables were investigated to determine the optimal magnetic solid phase extraction conditions. Results showed that the mMIPs had saturated adsorption capacities of 137.4, 78.7, 65.2, and 58.4 mg/g for trimethoprim, sulfadiazine, sulfamethazine, and sulfamethoxazole, respectively. Under optimized conditions, the linear range of the method for the detection of the 4 sulfonamides was 0.1–100 ng mL −1 ( R > 0.991), the detection limit was 0.015 μg kg −1 (S/N = 3), the recovery rate was 78.6–101%, and the relative standard deviation (RSD%) was 4.2–7.8%. This new rapid and ultra-sensitive pretreatment method was successfully applied to the monitoring of trace sulfonamide antibiotics residues in fish samples.
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高影响因子
GLYCOCONJUGATE JOURNAL
·
2017
·Vol. 34
·期 5
·625-632
·
IF 2.186
Song Shuang,Yu Qi,Zhang Bao,Ai Chunqing,Sun Yujiao,Fu Yinghuan,Zhao Meiyu,Wen Chengrong
Fish intestines and livers are usually considered as delicious and nutritious food in China. Acidic polysaccharides are important nutrients in these food of animal origin, but there is currently little information regarding their quantitative distributions. The present study demonstrated a method to quantify acidic polysaccharides simultaneously by analyzing their disaccharides produced from the acid hydrolysis using high-performance liquid chromatography (HPLC) coupled with triple quadrupole mass spectrometry. The recoveries for these acidic polysaccharides were all 97%–115% with relative standard deviation of 3.0%–9.0%. All of the acidic polysaccharides had good linearities. Then this method was applied to determine the composition of acidic polysaccharides in 5 edible fish livers and intestines. Besides well-known glycosaminoglycans (GAGs) including hyaluronic acid (HA), Chondroitin sulfate (CS), dermatan sulfate (DS) and heparin (HP), 4 novel acidic polysaccharides including 2 GAGs and 2 non-GAGs comprised of hexose-hexuronic acid repeating units were also found. CS and HP were the major acidic polysaccharides components in fish intestines and livers, respectively. The absolute amounts of acidic polysaccharides differed greatly in these fish tissues, but their proportions showed similarity in the same type of tissues. The present study demonstrated an effective method for acidic polysaccharides quantification, and revealed acidic polysaccharides compositions of edible fish livers and intestines.
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NanoImpact
·
2016
·
IF 0
Xiaomei Shi,Zaixing Li,Wei Chen,Liwen Qiang,Junchao Xia,Meng Chen,Lingyan Zhu,Pedro J.J. Alvarez
Wastewater treatment plants (WWTPs) can be important point sources for the discharge of engineered nanomaterials to natural surface waters. In this study, we investigated the fate of nano-TiO 2 in two full scale WWTPs and their receiving streams in northern China (i.e., the Binhe and Minxin rivers, which flow to the Xiaohe River). High resolution transmission electron microscopy , energy dispersion X-ray spectroscopy and X-ray photoelectron spectroscopy analyses indicated that Ti in colloids present in the WWTP effluents and receiving streams originated mainly from engineered nano-TiO 2 . Most of the nano-TiO 2 (74–85%) in the influent sewage was removed by the activated sludge , but significant concentrations (27–43 μg/L Ti) remained associated with colloids in the effluents. Surprisingly, the total concentration of Ti in the receiving river streams was higher (52–86 μg/L), indicating the importance of other sources such as urban runoff. These relatively high Ti concentrations are unlikely to originate from natural sources since they are significantly higher than those in the nearby Yuqiao Reservoir (< 5 μg/L), representing regional background levels. Consistently, Ti levels in fish collected from the Xiaohe River (including muscle and other tissues) were significantly higher than those of fish from the reservoir. Overall, TiO 2 releases through WWTP effluents and other sources (including runoff) are contributing to the relatively high observed concentrations of suspended Ti in the Xiaohe River, which results in accumulation in edible fish tissue.
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LWT-FOOD SCIENCE AND TECHNOLOGY
·
2017
·
IF 2.329
Huan Yang,Kun Feng,Peng Wen,Min-Hua Zong,Wen-Yong Lou,Hong Wu
A composite zein nanofibrous mat containing fish oil and ferulic acid was successfully fabricated by electrospinning. The process became more fluent and continuous by adding 30 g/L glycerol into the polymer solutions and using modified coaxial electrospinning. The average diameter of nanofibers was 440 nm. The loading capacity and encapsulation efficiency of fish oil were 20% and 94%, respectively. FTIR data demonstrated that fish oil and ferulic acid were successfully embedded into the nanofibers and there were interactions among the molecules of zein, fish oil and ferulic acid. Addition of ferulic acid into the nanofibers significantly improved the oxidative stability of encapsulated fish oil; moreover, it did not change the release behavior of fish oil. The release of encapsulated fish oil was controlled by a combination of diffusion and macromolecular chain relaxation. This composite nanofibrous mat with favorable oxidation stability and release property is potential in application as nutrition additive.
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高影响因子
INTERNATIONAL JOURNAL OF BIOLOGICAL MACROMOLECULES
·
2017
·
IF 3.671
Nan Wang,Ru-Na Jin,A.M. Omer,Xiao-kun Ouyang
In the present study, a new adsorbent based on carboxylated cellulose nanocrystal (CCN) was developed for the adsorption of Pb(II) from fish sauce. The prepared adsorbent material was characterized by zeta potential, FT-IR, XRD, and XPS tools. The changes in the morphological structure of the developed CCN surface were evidenced by SEM and TEM. The favorable adsorption conditions were selected by studying the contact time, initial concentration, temperature, and concentration of the used glutamic acid and NaCl. The results indicated that the Langmuir isotherm model agrees very well with experimental adsorption data ( R 2 = 0.9962) with a maximum adsorption capacity 232.56 mg/g of Pb(II) at 293.2 K. Additionally, data of the adsorption kinetics follow the pseudo-second-order kinetics ( R 2 > 0.9990). On the other hand, the thermodynamics studies show that the adsorption process is spontaneous and endothermic. Furthermore, the developed CCN could be regenerated using acid treatment with a good reusability for Pb(II) adsorption. The results clearly indicated that the synthesized CCN could be effectively applied as a new material for Pb(II) adsorption from fish sauce solutions.
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高影响因子
危险品化学品经营许可证（带存储）