FOOD CHEMISTRY
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2026
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IF 10.4
Wen Qing Jia,Jian Bing Li,Jiang Zhu,Qiao Wan,Zhen Chen,Lei Han
Noble-metal-free high-entropy alloy nanozyme was synthesized in a facile way. • A bioenzyme-free sensor was constructed for direct and rapid sensing of Gly. • This sensor shows high sensitivity and selectivity for Gly detection. • Smartphone-assisted hydrogel kit can realize point-of-care testing of Gly. It is urgently needed to exploit an effective technique for point-of-care testing (POCT) of glyphosate (Gly). Herein, we synthesized a high entropy alloy aerogel (FeCoNiCuMn HEAAs) nanozyme for quick and portable detection of Gly. The FeCoNiCuMn HEAAs with excellent peroxidase (POD)-mimetic activity can catalyze 3,3′,5,5′-tetramethylbenzidine (TMB) oxidation to blue oxTMB in the presence of H 2 O 2 . Gly can restrain the POD-mimetic activity of FeCoNiCuMn HEAAs due to the coordination between the FeCoNiCuMn HEAAs and Gly via P–O–Cu and P–O–Fe, leading to a decreased absorbance of the system. Leveraging this mechanism, a bioenzyme-free mediated colorimetric sensor was designed for Gly detection. This colorimetric sensor displays high specificity and sensitivity for Gly. Given its simplicity and bioenzyme-free participation, a smartphone-assisted hydrogel kit was also constructed for the POCT of Gly. The potential application of the colorimetric sensor and hydrogel kit was verified by detecting the Gly in foods. Download: Download high-res image (157KB) Download: Download full-size image
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ANALYTICAL AND BIOANALYTICAL CHEMISTRY
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2017
·Vol. 409
·期 30
·7133-7144
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IF 3.431
Zhang Chao,She Yongxin,Li Tengfei,Zhao Fengnian,Jin Maojun,Guo Yirong,Zheng Lufei,Wang Shanshan,Jin Fen,Shao Hua,Liu Haijin,Wang Jing
An electrochemical sensor based on molecularly imprinted polypyrrole (MIPPy) was developed for selective and sensitive detection of the herbicide glyphosate (Gly) in cucumber and tap water samples. The sensor was prepared via synthesis of molecularly imprinted polymers on a gold electrode in the presence of Gly as the template molecule and pyrrole as the functional monomer by cyclic voltammetry (CV). The sensor preparation conditions including the ratio of template to functional monomers, number of CV cycles in the electropolymerization process, the method of template removal, incubation time, and pH were optimized. Under the optimal experimental conditions, the DPV peak currents of hexacyanoferrate/hexacyanoferrite changed linearly with Gly concentration in the range from 5 to 800 ng mL −1 , with a detection limit of 0.27 ng mL −1 ( S/N = 3). The sensor was used to detect the concentration of Gly in cucumber and tap water samples, with recoveries ranging from 72.70 to 98.96%. The proposed sensor showed excellent selectivity, good stability and reversibility, and could detect the Gly in real samples rapidly and sensitively.
Schematic illustration of the experimental procedure to detect Gly using the MIPPy electrode
研究领域:
分析化学
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环境科学
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TALANTA
·
2023
·
IF 6.1
Qing Li,Yu-Meng Guo,Xiang-Yi He,Guang-Li Li
The reliable and accurate detection of glyphosate is urgently demanded because it is related to food and environmental safety. In this contribution, a PDA-PEI/Cu 2+ complex that possesses peroxidase-mimetic activity and stimulus-responsive fluorescence was fabricated by coordinating Cu 2+ with polydopamine-polyethyleneimine copolymer dots (PDA-PEI CPDs). With the introduction of Cu 2+ , the fluorescence intensity of PDA-PEI CPDs dropped sharply owing to the electron transfer effect. As a peroxidase-mimicking nanozyme , the PDA-PEI/Cu 2+ complex owns catalytic capacity to oxidize the colorless 3,3′,5,5′-tetramethylbenzidine (TMB) into blue oxTMB, leading a further fluorescence quenching by internal filtering effect by oxTMB. Once the glyphosate participated, the fluorescence signal of PDA-PEI CPDs is recovered significantly because of the formation of more stable Glyp-Cu 2+ complexes, meanwhile the peroxidase-mimicking activity of PDA-PEI/Cu 2+ complex could be strongly hindered. According to this principle, a novel and extremely convenient ‘turn off’ colorimetric and ‘turn on’ fluorescence sensing platform can be established for dual-mode detection of glyphosate. The favorable sensitivity and selectivity and were verified in the analysis of glyphosate in the environment through the marriage of dual-signal sensing platform. The detection limit of the dual-mode glyphosate sensing platform was 103.82 ng/mL for colorimetric assay and 16.87 ng/mL for fluorescent assay, respectively. Satisfactory recoveries in the range of 96.40%–104.66% were obtained, indicating the potential of this method for application in complicated real sample. Thereby, this strategy broadens the applications of polydopamine nanomaterials and holds a promising application in determination of pesticide residues .
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INTERNATIONAL JOURNAL OF BIOLOGICAL MACROMOLECULES
·
2023
·
IF 8.2
Fanping Kong,Qian Zhang,Yuan Xie,Jiyue Ding,Hui Zhao,Zihan Zhang,Zequn Ma,Hailin Cong,Zilin Meng
Nanocarrier preparations could effectively improve the utilization rate of pesticides, and reduce pesticide loss. In this study, glyphosate (GLY)-loaded Mg Al layered double hydroxide (GLY@LDH) was synthesized via an in-situ method. Subsequently, GLY@LDH composite samples were prepared using a layer-by-layer self-assembly approach and modified with poly-L-aspartic acid (PASP) and chitosan (CS). XRD, FT-IR, SEM, and Zeta potential characterization confirmed that GLY was successfully loaded in the interlayer of LDHs and PASP/CS were successfully encapsulated on the surface of the composite sample. The release effect in different ionic solutions and soils was studied and analyzed. The release behavior conforms to the Ritger-Peppas kinetic model, and the release mechanism was ion exchange, which was further explored by means of XRD, SEM, and molecular simulation. The results of the anti-scouring experiment and contact angle measurement indicated that the layered self-assembly material enhanced the washing resistance of the material. The practical application effect of the sample was verified through a pot experiment. This study provides new insights into the simple preparation of pesticide-controlled release formulations that reduce leaching losses.
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SEPARATION AND PURIFICATION TECHNOLOGY
·
2023
·
IF 8.6
Lei Jin,Yunlong Chen,Hailin Tian,Xiang Liu,Yingping Huang,Ruiping Li,Chuncheng Chen,Zhongxu Dai,Di Huang
The selective cleavage-bond of glyphosate is still a major challenge, especially in biodegradation and chemical degradation like advanced oxidation process (AOP). In this work, we found that the BiVO 4 and BiVO 4 /BiPO 4 heterogeneous photocatalysts generated different intermediates during the photocatalytic degradation of glyphosate. The main intermediate product by the BiVO 4 photocatalyst was aminomethylphosphonic acid (AMPA, reaching to 81%) with high biological toxicity, which means glyphosate was degraded through the AMPA pathway. While the BiVO 4 /BiPO 4 heterogeneous photocatalysts showed a significant inhibition of the accumulated AMPA (31%) at pH = 4, leading to the generation of benign products, such as sarcosine, acetic acid and PO 4 3- . More importantly, the BiVO 4 /BiPO 4 -2 photocatalyst barely generated AMPA (less than 5%) at pH = 10. The lower AMPA concentration in our experiments was not attributed to its rapid degradation rate because AMPA could not be degraded from acidic (pH = 4) to alkaline (pH = 10). It indicated that glyphosate suffered from two cleavage-bond pathways between the BiVO 4 and BiVO 4 /BiPO 4 heterogeneous photocatalysts, which had been proved by mass spectrometry (MS) results. The attenuated total reflectance Fourier transforms infrared (ATR-FTIR) spectrum and density function theory (DFT) demonstrated that the adsorption sites of the BiVO 4 /BiPO 4 -2 photocatalyst occurred on the phosphate group of glyphosate molecules at pH = 10, which facilitated the C–P bond cleavage and inhibited the formation of AMPA. We found that pH-mediated BiVO 4 /BiPO 4 dipole controlled and changed glyphosate electron cloud distribution, played an important role in the selective cleavage-bond of glyphosate. These results revealed both scientific and environmental significance for the efficient degradation of glyphosate through benign intermediates in soil and water.
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JOURNAL OF AGRICULTURAL AND FOOD CHEMISTRY
·
2021
·
IF 5.279
Danqiu Luo,Xiaohuan Huang,Bangyan Liu,Wenying Zou,Yuangen Wu
The rapid and onsite detection of glyphosate herbicides in agricultural products is still a challenge. Herein, a novel colorimetric nanozyme sheet for the rapid detection of glyphosate has been successfully prepared through the physical adsorption of porous Co3O4 nanoplates on a polyester fiber membrane. Glyphosate can specifically inhibit the peroxidase-mimicking catalytic activity of porous Co3O4 nanoplates, thereby the visual detection of glyphosate can be realized by distinguishing the change in the color intensity of the established nanozyme sheet. The prepared nanozyme sheet has good sensitivity and selectivity, with a detection limit of 0.175 mg·kg–1 for glyphosate detection just by the naked eyes. It can effectively detect glyphosate within 10 min, and the color spots can maintain more than 20 min. The nanozyme sheet is not easily affected by the external environment in detection and storage. The merits of the nanozyme sheet facilitate its practical application in the large-scale preliminary screening of glyphosate residues in agricultural products.
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RSC Advances
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2022
·Vol. 12
·期 5
·2623-2631
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IF 4.036
Kexin Zhou,Xing-peng Liu,Hongyun Guo,Hui-qiang Li,Ping Yang
Heterogeneous electro-Fenton is one of the promising technologies to degrade refractory organic phosphonates. In this work, CoNWs@CoAl-LDHs/Fe3O4 and CoNPs@CoAl-LDHs/Fe3O4 were successfully synthesized by a co-precipitation method and applied to degrade 1-hydroxyethane-1,1-diphosphonic acid (HEDP) and glyphosate (PMG) via an electro-Fenton process. The results indicated that the removal rate of HEDP (100 mg L−1) and PMG (100 mg L−1) by CoNWs@CoAl-LDHs/Fe3O4 increased from 62.09% and 95.31% to 82.45% and 100%, respectively. The CoNPs@CoAl-LDHs/Fe3O4 electro-Fenton system could remove 70.03% of HEDP and nearly 100% of PMG within 2 hours at a pH of 3. Moreover, we compared the SEM, EDS, XRD and BET results of CoNWs@CoAl-LDHs/Fe3O4 with those of CoNPs@CoAl-LDHs/Fe3O4. The effects of initial pH, CoNW dosage and reaction time on the degradation of HEDP and PMG were discussed. CoNWs@CoAl-LDHs@Fe3O4 could even remove 71.03% of HEDP at a neutral pH. After four cycles of repeated use at a pH of 3, the removal rate of HEDP by CoNWs@CoAl-LDHs/Fe3O4 was still higher than 70%. Radical quenching experiments revealed that ˙OH is the dominant active species participating in the heterogeneous electro-Fenton process. Finally, we would talk about the mechanism of the CoNWs@CoAl-LDHs/Fe3O4-based electro-Fenton system.
研究领域:
环境科学
·
材料科学
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AQUATIC TOXICOLOGY
·
2026
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IF 4.3
Junyue Zheng,Chen Wang,Miaomiao Teng,Tong Li,Jiazhen Jiang,Xiangguang Chen,Fengchang Wu
Glyphosate (Gly) is the most commonly used herbicide in the world. Glyphosate and its main degradation product, aminomethylphosphonic acid (AMPA), are often detected in aquatic environments and may pose a threat to the health of aquatic organisms. It is well established that Gly is mainly enriched in the intestine and degraded into AMPA. However, it remains unclear whether they disrupt the gut microbiota and cause liver health effects, as well as whether they have a synergistic effect with antibiotics. We investigated herein the effects of zebrafish exposure to Gly, AMPA and oxytetracycline (OTC) alone or in combination on the gut-liver axis. Our results suggest that Gly, AMPA, and OTC induce lipid accumulation, inflammatory response and oxidative damage in the liver, which is associated with a higher Firmicutes/Bacteroidetes ratio in the intestine and a higher levels of lipopolysaccharide in serum. Compared with the single exposure groups (OTC, Gly, and AMPA), the toxic effects of the gut-liver axis were more severe in the combined exposure groups (Gly+OTC, AMPA+OTC). Our results indicate that the health risks posed by Gly, AMPA and OTC through the gut microbiota of aquatic organisms are a neglected hot spots.
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MICROCHEMICAL JOURNAL
·
2025
·
IF 5.1
Xiudan Hou,Zirou Wang,Hui Yu,Yufei Liu,Qingli Yang
Excess residues of organophosphorus pesticides in traditional Chinese medicinal herbs has been driving the development of rapid and specific detection methods. Herein, a dual-mode sensing platform was established for the simultaneous determination of glyphosate and profenofos using the colorimetric and fluorescence sensors, respectively. A Zn-incorporated porous coordination network-224 (PCN-224) was prepared to selectively adsorb glyphosate based on the coordination effect of Zr and phosphate ion, which would inhibit the peroxidase-like activity of PCN-224(Zn). An aptamer-modified nitrogen-carbon quantum dots (N-CQDs) was used to specifically recognize profenofos, further adopting the quenching effect of PCN-224(Zn) towards N-CQDs to realize the quantitative analysis. The colorimetric sensing mechanism was also studied. Coupled with a smartphone application, the established method exhibited the linearity of 0.06–3.1 μg mL −1 (glyphosate), and 0.02–3.9 μg mL −1 (profenofos). The matrix effect was 100.58 % and 95.46 % in Angelica sinensis and Codonopsis , presenting a new horizon for the multi-model sensor in real samples.
研究领域:
分析化学
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环境科学
·
药学
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JOURNAL OF FOOD COMPOSITION AND ANALYSIS
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2025
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IF 4.6
Yi He,Runzi Zhang,Shunbi Xie,Xiang He,Xiaoyu Yang,Yao Liu,Mengjun Wang
Food safety has become a significant global concern, with particular attention on pesticide residues in agricultural products. Glyphosate, as a pesticide, poses a serious threat to human health and the environment when present as residue in agricultural products. In this study, we utilized MOF as a framework to prepare concave-structured bimetallic prussian blue nanozyme@silver (Co-Fe PBA@Ag). This nanozyme with sunken surface and synergistic effect of Ag nanoparticles exhibited high catalytic activity than common nanozyme and could catalyze TMB to generate oxTMB under the action of hydrogen peroxide, resulting in a color change from colorless to blue in the solution. However, when the target glyphosate was present, it could inhibit the activity of OH · and consequently blocked the color change reaction, causing the solution to turn from blue to colorless. Based on this principle, we established a triple-mode detection system: colorimetry, smartphone app analysis, and SERS for detecting glyphosate residues in agricultural products such as honey, potatoes, and tea leaves. This triple-mode sensor integrated point-of-care detection capability via colorimetry, sensitive monitoring through smartphone app and accurate detection using Raman technology. It showed potential for the implementation of practical on-site monitoring of pesticide residues in agricultural products.
研究领域:
食品安全
·
纳米生物学
·
环境科学
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ANALYTICA CHIMICA ACTA
·
2024
·
IF 5.7
Nan Zhang,Shuangming Guo,Yaxiling Wang,Chunyuan Zhu,Peiwen Hu,Hong Yang
Background Presently, glyphosate (Gly) is the most extensively used herbicide globally, Nevertheless, its excessive usage has increased its accumulation in off-target locations, and aroused concerns for food and environmental safety. Commonly used detection methods, such as high-performance liquid chromatography and gas chromatography , have limitations due to expensive instruments, complex pre-processing steps, and inadequate sensitivity. Therefore, a facile, sensitive, and reliable Gly detection method should be developed. Results A photoelectrochemical (PEC) sensor consisting of a three-dimensional polymer phenylethnylcopper/nitrogen-doped graphene aerogel (PPhECu/3DNGA) electrode coupled with Fe 3 O 4 NPs nanozyme was constructed for sensitive detection of Gly. The microscopic 3D network of electrodes offered fast transfer routes for photo-generated electrons and a large surface area for nanozyme loading, allowing high signal output and analytical sensitivity. Furthermore, the use of peroxidase-mimicking Fe 3 O 4 NPs instead of natural enzyme improved the stability of the sensor against ambient temperature changes. Based on the inhibitory effect of Gly on the catalytic activity Fe 3 O 4 NPs, the protocol achieved Gly detection in the range of 5 × 10 −10 to 1 × 10 −4 mol L −1 . Additionally, feasibility of the detection was confirmed in real agricultural matrix including tea, maize seedlings, maize seeds and soil. Significance This work achieved facile, sensitive and reliable analysis towards Gly, and it was expected to inspire the design and utilization of 3D architectures in monitoring agricultural chemicals in food and environmental matrix.
研究领域:
环境科学
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分析化学
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JOURNAL OF AGRICULTURAL AND FOOD CHEMISTRY
·
2021
·
IF 5.279
Yong Wang,Wanjun Gao,Yeyun Li,Yu Xiao,Wei Song,Ting Yao,Manhuan Cheng,Wenjuan Wang,Ruyan Hou
The tea shrub is grown in long-standing orchards, an environment that is suitable for persistent weed growth, which is increasingly controlled by herbicides. Therefore, there is increasing concern that tea consumers may be exposed to herbicide residues. In this study, the levels of glufosinate-ammonium (GLU), glyphosate [N-(phosphonomethyl) glycine; PMG], and its metabolite aminomethyl phosphoric acid (AMPA) were determined in tea samples by HPLC–MS/MS using several current purification methods and a new method that we developed herein. The matrix effect of our proposed method was between -27.3 and 27.7%, which was lower than that in other methods, indicating that this method effectively reduced the interference of tea matrix in the mass spectrometry process. This method was used to determine the levels of PMG, GLU, and AMPA in 780 samples, including six traditional Chinese teas (green tea, black tea, oolong tea, dark tea, white tea, and yellow tea) and a floral tea, from 14 provinces of China. Probability estimates showed that the 95th percentile risk entropy values of the three pesticide residues were far below the acceptable risk level. The risk assessment results showed that exposure to PMG, GLU, and AMPA caused by drinking tea beverages poses no significant risk to human health.
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