FOOD CHEMISTRY
·
2024
·
IF 8.5
Min Zhuang,Jiaqing Li,Anqi Wang,Gaoheng Li,Sheng Ke,Xuanyu Wang,Ming Ning,Zhanwu Sheng,Bing Wang,Zhongkai Zhou
Bioactive peptide’s development is facing two challenges in terms of its lower yield and limited understanding of structurally orientated functionality. Therefore, peptides were prepared from wheat bran via a cocktail enzyme for achieving a higher level of hydrophobic amino acids than traditional method. The obtained peptides exhibited great antioxidant activities against H 2 O 2 -induced oxidative stress in HepG2 cells. Among them, 91 bioactive peptides were selected through the virtual screening, and their N -terminal and C -terminal contained many hydrophobic amino acids. Then the peptides with capacity to interact with Keap1 were identified by in silico simulation, because Keap1 acts as a sensor of redox insults. The results revealed that peptides DLDW and DLGL demonstrated the highest binding affinities, and a bridge was formed between Asp of DLGL and Arg415 of Klech domain, contributing to interfering Keap1-Nrf2 interaction. These findings implied a potential application of wheat bran peptides as nutraceuticals and health-promoting ingredients.
研究领域:
食品科学
·
分子生物学
·
营养保健品
·
药理学
✓ 已验证
产品关联
DOI
PubMed
高影响因子
Advanced Science
·
2024
·
IF 14.3
Wen Zhou,Yuwan Chen,Wenxin Fu,Xinwei Li,Yufei Xia,Qun Zhao,Baofeng Zhao,Yukui Zhang,Kaiguang Yang,Lihua Zhang
The study of in situ conformations and interactions of mitochondrial proteins plays a crucial role in understanding their biological functions. Current chemical cross-linking mass spectrometry (CX-MS) has difficulty in achieving in-depth analysis of mitochondrial proteins for cells without genetic modification. Herein, this work develops the reactive oxygen species (ROS)-responsive cross-linker delivery nanoparticles (R-CDNP) targeting mitochondria. R-CDNP contains mitochondria-targeting module triphenylphosphine, ROS-responsive module thioketal, loading module poly(lactic-co-glycolic acid) (PLGA), and polyethylene glycol (PEG), and cross-linker module disuccinimidyl suberate (DSS). After targeting mitochondria, ROS-triggered cross-linker release improves the cross-linking coverage of mitochondria in situ. In total, this work identifies 2103 cross-linked sites of 572 mitochondrial proteins in HepG2 cells. 1718 intra-links reveal dynamic conformations involving chaperones with ATP-dependent conformation cycles, and 385 inter-links reveal dynamic interactions involving OXPHOS complexes and 27 pairs of possible potential interactions. These results signify that R-CDNP can achieve dynamic conformation and interaction analysis of mitochondrial proteins in living cells, thereby contributing to a better understanding of their biological functions.
研究领域:
生物化学
·
细胞生物学
·
分子生物学
·
纳米技术
✓ 已验证
产品关联
DOI
PubMed
高影响因子
Food Bioscience
·
2024
·
IF 4.8
Cong Teng,Suling Li,Lujing Xu,Kaiyang Ma,Yifei Lu,Jin Feng,Zhi Chai,Xindi Hu,Wei Zhou,Ying Li
A sulfate polysaccharide fraction (PYSP) was extracted and purified through DEAE cellucose-52 and Sephadex G-100 from Porphyra yezoensis . The PYSP is an acidic polysaccharide with a Mw of 152.93 kDa. PYSP was composed of galactose, glucose, and Glc-UA sugars with a molar ratio of 82.38:5.02:12.59. PYSP has an irregular fragmentation structure, with a sulfuric acid group content of 43.93% ± 0.47%. It suggested that PYSP has effective cholate and cholesterol adsorption capacity, α-glucosidase inhibition activity. When the concentration of PYSP was 1.0 mg/mL, the inhibition rates of α-glucosidase were 45.02%. PYSP could decrease α-helix and increase β-fold and random curl of α-glucosidase. In addition, the in vitro hypoglycemic activities of PYSP were dose-dependent. PYSP could improve glucose consumption, glycogen synthesis, and pyruvate kinase activity in insulin-resistant HepG2 cells. These results suggested that PYSP is a novel material for functional hypoglycemic foods.
研究领域:
海洋生物学
·
生物化学
·
药理学
·
食品科学
✓ 已验证
产品关联
DOI
高影响因子
Foods
·
2022
·Vol. 11
·期 12
·1771
·
IF 5.561
Peichun Lin,Suhua Chen,Siyan Zhong
Fucoidan is a marine sulfated polysaccharide that is rich inSargassumand has a wide range of biological activities. In this study, the chemical composition and bile acid binding ability of six crude fucoidans were compared, the nutrition and chemical composition ofSargassum zhangiiwere analyzed, and fucoidan fromSargassum zhangiiwas extracted and purified. The purified fractions (ZF1, ZF2, and ZF3) were analyzed by physicochemical characterization, and the ability of binding bile acid and cholesterol lowering in HepG2 cells were evaluated. The results showed that the contents of sulfate in crude fucoidan fromSargassum Zhangii(ZF) was as high as13.63%. Its ability of binding bile acid was better than other five crude fucoidans.Sargassum zhangiiwas a kind of brown seaweed with high carbohydrate, and low fat and rich in minerals. The sulfate content of ZF1, ZF2, and ZF3 was 3.29%, 19.39%, and 18.89% respectively, and the molecular weight (Mw) was 4.026 × 105, 2.893 × 105, and 3.368 × 105, respectively. Three fucoidans all contained the characteristic absorption bands of polysaccharides and sulfate groups and were rich in fucose. Three fucoidans can bind to bile acid, and ZF2 showed the best binding capability. In vitro experiments showed that ZF1, ZF2, and ZF3 could reduce intracellular total cholesterol (TC) content in HepG2 cells without affecting their viability. ZF2 showed the best ability to reduce TC.Keywords:Sargassum zhangii;nutritional and chemical composition;fucoidan;physicochemical characterization;bile acid;cholesterol-lowering activity
研究领域:
海洋生物学
·
生物化学
·
药理学
✓ 已验证
DOI
PubMed
高影响因子
Frontiers in Pharmacology
·
2022
·
IF 5.988
Zhang Xuguang,Zhang Yuxin,Zhou Mingyan,Xie Yiqiang,Dong Xiujuan,Bai Feihu,Zhang Junqing
(R)-5-hydroxy-1,7-diphenyl-3-heptanone (DPHC) from the natural plant Alpinia officinarum has been reported to have antioxidation and antidiabetic effects. In this study, the therapeutic effect and molecular mechanism of DPHC on type 2 diabetes mellitus (T2DM) were investigated based on the regulation of oxidative stress and insulin resistance (IR) in vivo and in vitro. In vivo, the fasting blood glucose (FBG) level of db/db mice was significantly reduced with improved glucose tolerance and insulin sensitivity after 8 weeks of treatment with DPHC. In vitro, DPHC ameliorated IR because of its increasing glucose consumption and glucose uptake of IR-HepG2 cells induced by high glucose. In addition, in vitro and in vivo experiments showed that DPHC could regulate the antioxidant enzyme levels including superoxide dismutase (SOD), catalase (CAT), and glutathione peroxidase (GSH-Px), thereby reducing the occurrence of oxidative stress and improving insulin resistance. Western blotting and polymerase chain reaction results showed that DPHC could promote the expressions of nuclear factor erythroid 2-related factor 2 (Nrf2), the heme oxygenase-1 (HO-1), protein kinase B (AKT), and glucose transporter type 4 (GLUT4), and reduced the phosphorylation levels of c-Jun N-terminal kinase (JNK) and insulin receptor substrate-1 (IRS-1) on Ser307 both in vivo and in vitro. These findings verified that DPHC has the potential to relieve oxidative stress and IR to cure T2DM by activating Nrf2/ARE signaling pathway in db/db mice and IR-HepG2 cells.
研究领域:
药理学
·
分子生物学
·
糖尿病研究
·
细胞生物学
✓ 已验证
DOI
PubMed
高影响因子
Foods
·
2026
·Vol. 15
·期 8
·1344
·
IF 6
Ningxin Ding,Xiangyu Pang,Yixuan Yan,Mingcong Fan,Haifeng Qian,Li Wang,Yan Li
Hypercholesterolemia is a major modifiable risk factor for cardiovascular disease. Although germinated brown rice (GBR) is rich in bioactive constituents and has shown preliminary lipid-regulating potential, the active compounds and underlying mechanisms remain unclear. Therefore, this study comparatively investigated extracts from four GBR cultivars and evaluated their cholesterol-regulating activity using untargeted metabolomics in combination with a cholesterol-induced HepG2 cell model. After 36 h of germination, the extracts showed significantly increased total phenolic content, total flavonoid content, and antioxidant activities (p< 0.05), with cultivar Q showing the strongest response. In the in vitro cholesterol-induced HepG2 cell model, GBR extract at 150 μg/mL significantly reduced intracellular total cholesterol (TC), triglyceride (TG), and low-density lipoprotein cholesterol (LDL-C) levels by approximately 69%, 73%, and 46%, respectively, while increasing high-density lipoprotein cholesterol (HDL-C) levels by approximately 125% (allp< 0.05). Untargeted metabolomics and subsequent validation suggested that p-coumaric acid is a candidate bioactive compound contributing to the cholesterol-lowering effect of GBR. Further analysis indicated that this effect may be associated with modulation of the mRNA expression levels of SREBP2, HMGCR, and LDLR. These findings indicate that germination enhances the bioactivity of brown rice in a cultivar-dependent manner and provides supporting evidence for the potential application of GBR as a functional food ingredient for lipid management.
✓ 已验证
DOI
PubMed
高影响因子
Journal of Functional Foods
·
2023
·
IF 5.6
Kun Hu,Yongjin Sun,Jie Wang,Shaojun Wu,Jie Ren,Dan Su,Lidan Tang,Jinhong Gong,Hufeng Fang,Shan Xu,Hao Yang
Hepatocyte lipid accumulation in type 2 diabetes mellitus (T2DM) promotes the development of metabolic dysfunction-associated steatosis liver disease (MASLD), whereas no specific drug treatment is available. Pinocembrin assists in the treatment of many diseases, however, little is known about its protective effect on hepatic lipid metabolism disorders induced by T2DM. In this study, the ameliorative effects of pinocembrin on T2DM-induced hepatocyte lipid accumulation were investigated by in vitro experiments combined with a comprehensive analysis strategy of network pharmacology and molecular docking. High glucose and free fatty acid co-cultured HepG2 cell line was used. The results indicated that pinocembrin reduced lipid deposition by modulating the release of Cyclooxygenase-2 (COX-2)-mediated inflammatory factors and improving insulin resistance (IR) in HepG2 cells. Overall, the findings of this study suggested that pinocembrin ameliorated lipid accumulation and IR in vitro , and thus may be a potential therapeutic agent for the treatment of T2DM-induced hepatic steatosis.
✓ 已验证
产品关联
DOI
高影响因子
LWT-FOOD SCIENCE AND TECHNOLOGY
·
2024
·
IF 6
Yan Zhang,Yuyang Liu,Wannan Li,Weiwei Han
Antioxidant peptides can be obtained from diverse dietary protein sources with high safety. The Kelch-like ECH-associated protein 1(Keap1) is a key protein in the cellular oxidative stress signaling pathway and it plays an important role in the field of antioxidant. Soybeans are a vital source for the production of bioactive peptides. In this study, firstly, we screened 468 antioxidant peptides from soybean protein with low molecular weight, high hydrophobicity and non-toxicity. The top three antioxidant peptides were isolated and extracted from the fractions using UHPLC-QQQ/MS analysis. The free radical scavenging rate demonstrated significant antioxidant activity of PHHADS (EC 50 = 4.26 mmol/L). In addition, peptide PHHADS was demonstrated significantly protects HepG2 cells from oxidative stress-induced damage. Lastly, 200 ns molecular dynamics simulations were performed between the peptides and Keap1. The results revealed that PHHADS closely interacts with the active site of Keap1. Moreover, the interaction of the antioxidant peptides with Keap1 led to the disruption of the β-sheet structure of Keap1 in residues380-390, transforming into an irregularly curved structure. This particular region in Keap1 is crucial for substrates binding, suggesting a potential inhibitory mechanism by the peptides. These findings contribute to the understanding of antioxidant peptide interactions and their potential application in developing functional foods for health benefits.
研究领域:
食品科学
·
生物化学
·
分子生物学
·
药理学
✓ 已验证
产品关联
DOI
高影响因子
MOLECULES
·
2024
·Vol. 29
·期 17
·3990
·
IF 4.2
Shuang Tian,Yinxu Zhao,Siqi Deng,Liman Hou,Juan Song,Ming Wang,Ming Bu
In the following study, a series of new lupeol-3-carbamate derivatives were synthesized, and the structures of all the newly derived compounds were characterized. The new compounds were screened to determine their anti-proliferative activity against human lung cancer cell line A549, human liver cancer cell line HepG2, and human breast cancer cell line MCF-7. Most of the compounds were found to show better anti-proliferative activity in vitro than lupeol. Among them, obvious anti-proliferation activity (IC50= 5.39~9.43 μM) was exhibited by compound3iagainst all three tumor cell lines. In addition, a salt reaction was performed on compound3k(IC50= 13.98 μM) and it was observed that the anti-proliferative activity and water solubility of compound3k·CH3I(IC50= 3.13 μM), were significantly enhanced subsequent to the salt formation process. The preliminary mechanistic studies demonstrated that apoptosis in HepG2 cells was induced by compound3k·CH3Ithrough the inhibition of the PI3K/AKT/mTOR pathway. In conclusion, a series of new lupeol-3-carbamate derivatives were synthesized via the structural modification of the C-3 site of lupeol, thus laying a theoretical foundation for the design of this new anticancer drug.
研究领域:
药理学
·
肿瘤学
·
药物化学
✓ 已验证
DOI
PubMed
高影响因子
BIOMEDICAL CHROMATOGRAPHY
·
2024
·
IF 1.8
Lu-Lu Yang,Qi-Qi Zhou,Song Fan,Chun-Lu Liu,Hui-Jun Li
The saponin-enriched extract from Celosiae Semen is a promising resource owing to its lipid-lowering activity. However, triterpenoid saponins are difficult to extract owing to their high molecular weight and strong water solubility. The aim of this paper was to explore an eco-friendly and effective technology of extraction and enrichment of total triterpenoid saponins to obtain high lipid-lowering fractions. Initially, Box–Behnken design experiments were employed to optimize the heat reflux extraction process on the basic of mono-factor experiments. Afterwards, the crude extract was further purified using D-101 resin, and the purification parameters were investigated based on adsorption/desorption experiments and biological activity assay. Under optimal conditions, the purity of the finally obtained total triterpenoid saponins was increased by 7.28-fold. The lipid-lowering activities of the six main triterpenoid saponins were evaluated in HepG2 cells induced by palmitic acid. The results of Oil Red O staining showed that the compounds all exhibited potential lipid-lowering activity. The structure–activity relationship analysis suggested that the oligosaccharide chain at C-28 played an essential role in their lipid-lowering activity and the substituent group at C-23 site also showed important effects. The optimal extraction and purification methods may facilitate the utilization of Celosiae Semen for the industrial production as a functional food and drug.
研究领域:
药学
·
生物化学
·
食品科学
·
天然产物化学
✓ 已验证
产品关联
DOI
PubMed
高影响因子
Royal Society Open Science
·
2018
·Vol. 5
·期 11
·
IF 2.504
Shuqian Wan,Long Zhang,Yunyun Quan,Kun Wei
Resveratrol (3, 4′, 5-trihydroxy-trans-stilbene, RSV), a nutraceutical, has recently attracted lots of attention because of its outstanding pharmacological potential. The effects of RSV on non-alcoholic fatty liver disease (NAFLD) remain inconclusive, although a wealth of research has been done. The major obstacle presented was RSV's poor bioavailability due to its poor aqueous solubility, chemical instability and intestinal metabolism. In this study, nanotechnology was used to encapsulate RSV to enhance its stability, water solubility and bioactivity, which can be used to treat NAFLD by HepG2 hepatocytes-induced in vitro. RSV-loaded poly (d, l-lactide-co-glycolide acid) (PLGA) nanoparticles (RSV-PLGA-NPs) were prepared according to an oil/water (O/W) emulsion technique. The RSV-PLGA-NPs were of spherical morphology with an average size of 176.1 nm and a negative charge of −22.6 mV. These nanoparticles exhibited remarkable encapsulation efficiency (EE%) (97.25%) and drug loading (14.9%) for RSV. A sustained RSV release from RSV-PLGA-NPs could be achieved especially in acidic conditions when simulating transporting through the gastrointestinal tract. In addition, these nanoparticles were stable enough to store at 4°C for a least six months with unchanged EE%. Moreover, RSV-PLGA-NPs were more efficient in alleviating lipogenesis, promoting lipolysis and reducing hepatocellular proliferation than free RSV due to its improved stability, water solubility and bioactivity. These findings indicated that the RSV-PLGA-NPs provided superb and stable drug delivery with small particle size, high capsulation efficiency, well-controlled drug release, which greatly enhanced the stability, water solubility and bioactivity. Besides, the discovery that the inhibitory effect of RSV-PLGA-NPs on hepatocellular proliferation and lipid accumulation in steatotic HepG2 cells may provide a new way to study the mechanism of NAFLD. Therefore, RSV-PLGA-NPs have a promising potential for NAFLD therapy.
研究领域:
药理学
·
纳米技术
·
肝病学
·
药物递送系统
✓ 已验证
DOI
PubMed
高影响因子
MICROCHEMICAL JOURNAL
·
2024
·
IF 4.9
Luyan Meng,Bing Wang,Sijia Zhang,Shun Zhang,Ting Cai,Chuan-Fan Ding,Yinghua Yan
A Ti 4+ -functionalized ionic liquid polymer microsphere was proposed and prepared through free radical precipitation polymerization. The polymer microsphere demonstrates excellent performance on phosphopeptide enrichment, with a low detection limit (0.5 fmol) and good selectivity (1:2500). Through the analysis of HepG2 cell lysate using nano LC-MS/MS after material enrichment, a total of 1100 phosphopeptides and 733 phosphoproteins were identified. Additionally, in the serum of liver cancer patients, 69 phosphopeptides, 47 phosphoproteins, and 52 phosphorylation sites were identified, while healthy controls exhibited 55 phosphopeptides, 31 phosphoproteins, and 40 phosphorylation sites. Label-free quantification revealed 10 differentially expressed proteins (DEPs), with five proteins (FN1, SERPIND1, SERPINF2, IGFBP3, and MENT) being up-regulated and five proteins (SELENOP, PLG, SPP2, SERPINC1, and GP1BB) being down-regulated. These findings highlight the potential of the MBA-VImDOPA-Ti 4+ polymer microsphere in specifically enriching phosphopeptides from clinical biological samples.
研究领域:
分析化学
·
生物化学
·
蛋白质组学
·
癌症研究
✓ 已验证
产品关联
DOI
高影响因子
危险品化学品经营许可证（带存储）