ANALYST
·
2021
·Vol. 146
·期 8
·2664-2669
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IF 4.616
Liling Lu,Xiao Han,Jingwen Lin,Yingxin Zhang,Minghao Qiu,Ying Chen,Meijin Li,Dianping Tang
Herein a rapid and sensitive fluorometric bioanalysis platform for mercury(II) (Hg2+) detection was innovatively developed using ultrathin two-dimensional MXenes (Ti3C2) as fluorescence quencher and Hg2+-induced exonuclease III (Exo III)-assisted target recycling strategy for efficient signal amplification. Initially, fluorophore-labeled single-stranded DNA (FAM-labeled probe) can be easily adsorbed onto the surface of ultrathin Ti3C2 nanosheets by hydrogen bonding and metal chelating interaction, and the fluorescence signal emitted by the FAM-labeled probe is quenched strongly owing to the fluorescence resonance energy transfer between the FAM and ultrathin Ti3C2 nanosheets. Upon sensing the target Hg2+, the protruding DNA fragment at the 3′ end of hairpin will hybridize with primer (hairpin-Hg2+-primer), and then further digested by Exo III to produce a probe (nicker). The released target Hg2+ and primer continue to participate in the next recycling, resulting in more hairpin probes becoming nickers. The combination of a large number of nickers and FAM-probe resulted in a significant increase in the fluorescence signal of the system, which was attributed to the fact that the double helix DNA was more rigid and separated from the surface of the ultrathin Ti3C2 nanosheets. The obvious fluorescence signal change of the Ti3C2-based Exo III-assisted target recycling can be accurately monitored by fluorescence spectrometry, which is also proportional to the concentration of Hg2+. Under optimum operating conditions, the peak intensity (520 nm wavelength) of fluorescence increased with increasing Hg2+ within a wide dynamic working range from 0.05 nM to 50 nM (R2 = 0.9913) with a limit of detection down to 42.5 pM. The proposed strategy uses ultrathin MXenes as a platform for binding nucleic acids, which contributes to its potential in nucleic acid hybridization-based biosensing and/or nucleic acid signal amplification bio-applications.
研究领域:
分析化学
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环境科学
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纳米技术
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生物传感
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ACS Applied Nano Materials
·
2022
·
IF 6.14
Jiaojiao Zhou,Weijia Zhu,Xuqin Lv,Xiaoping Du,Jiangling He,Jie Cai
Mercury ion (Hg2+) is toxic and can severely damage the environment and human health. Therefore, sensitive and selective Hg2+ detection methods are needed. Here, we synthesized dendritic silica nanospheres (DSN) with central-radial porous structures and immobilized Au and Pt nanoparticles into their pores. The synthesized DSN with Au and Pt nanoparticles (AuPt@DSN) exhibited superior peroxidase-like activity, stability, and dispersibility. We found that AuPt@DSN can specifically reduce Hg2+, leading to the decreased catalytic activity of the nanozymes. Under optimal testing conditions, the AuPt@DSN material was efficiently used for the visual detection of Hg2+. The detection limit was 8.58 pM with a linear range from 0.1 nM to 10 μM. Selectivity toward Hg2+ has also been demonstrated in aqueous solutions in the presence of other metal ions. This strategy is simple to utilize and has a low cost and rapid response. The practicability was further investigated by analyzing complex water samples, which suggests its potential for mercury evaluation in environmental samples.
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CARBOHYDRATE POLYMERS
·
2022
·
IF 10.723
Congwei Li,Lian Duan,Xinjian Cheng
Fluorescent chitosan-based hydrogel for the selective detection and adsorption of Hg 2+ /Hg + in aqueous environment was prepared through three-step synthesis strategy. NO 2 -Boron-dipyrrolemethene (BODIPY) was prepared firstly, and then the -NO 2 group was reduced to -NH 2 group. Finally, the NH 2 -BODIPY was introduced to chitosan by Schiff base formation reaction through bi-aldehyde. Eventually, fluorescent chitosan hydrogel was obtained. The as-prepared fluorescent hydrogel probe could detect Hg 2+ /Hg + through PET mechanism with the detection limit of 0.3 μM. The recognition site which combines Hg 2+ /Hg + is C N, it is just formed in the reaction with chitosan and the amino group on BODIPY. Adsorption capacity of the fluorescent hydrogel is 121 mg·g −1 , which is almost seven times of the original chitosan. The isotherm and kinetics of Hg 2+ /Hg + removal follows Langmuir isotherm and pseudo-second order kinetics , respectively. Besides, a series of fluorescent hydrogels were prepared to compare the elasticity, hydropHilicity , fluorescence intensity and adsorption capacity.
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SENSORS AND ACTUATORS B-CHEMICAL
·
2014
·
IF 3.84
Haixia Zhang,Cuiyan Tong,Jie Sha,Bingxin Liu,Changli Lü
This paper reported a facile method for fabricating a functionalized fluorescent graphene oxide (GO) by covalently grafting the amine-modified mesoporous silica nanoparticles (MSNs) containing poly(p-phenylenevinylene) (PPV) and lactam of Rhodamine 6G (SRh6G) inside the channel of MSNs. The resulted GO-PPV@MSN-NH 2 @SRh6G fluorescent hybrid nanoparticles had a good dispersion and strong green fluorescence in aqueous solution. This hybrid material can be used as a fluorescent ratiometric probe to detect Hg 2+ in the water through fluorescence resonance energy transfer (FRET). Upon the addition of only Hg 2+ ions, the mission corresponding to the ring-opened form of rhodamine appeared at 550 nm with a simultaneous emission quenching of PPV at 494 nm. This ratiometric fluorescence change indicated that an FRET from the PPV to rhodamine was triggered by Hg 2+ ions. The hybrid nanoparticles of GO-PPV@MSN-NH 2 @SRh6G could be used as a novel fluorescent probe to carry out the quantitative detection of Hg 2+ with a detection limit of 7.1 × 10 −8 M.
研究领域:
化学
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环境科学
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材料科学
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JOURNAL OF PHOTOCHEMISTRY AND PHOTOBIOLOGY A-CHEMISTRY
·
2024
·
IF 4.1
Jinting Zhu,Linhan Fan,Wenqing Li,Xinru Qi,Changyan Sun,Wenjun Li,Zhidong Chang
The accumulation of heavy metal ions in nature will not only lead to the further deterioration of the environment, but also directly do harm to human health. And as a gas pollutant, excessive inhalation of formaldehyde is also harmful to human health. Herein, a novel Eu-MOF was synthesized by solvothermal method using Eu 3+ , 2-pyridine-4-yl-3H-benzimidazole-5-carboxylic acid (HL) and 1,10-phenanthroline (phen), which can be used as a ratio fluorescent probe to detect Hg 2+ , Cd 2+ and formaldehyde, and the limits of detection(LOD) can reach 34, 9.91 and 194 nM respectively. Among them, the LOD of Eu-MOF for Cd 2+ has reached the lowest level at present. Noticeable, Eu-MOF can detect formaldehyde in both liquid and gas phases, and the response time is extremely short. In addition, Eu-MOF has been applied to trace detection of Hg 2+ and Cd 2+ in tap water, lake water and sea water with satisfactory recoveries (ranging from 98.10 % to 103.0 %). A program has been developed for quickly calculating the concentration of the substances to be detected by capturing the fluorescent color of the solvents under the ultraviolet light.
研究领域:
环境科学
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分析化学
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材料科学
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OPTICAL MATERIALS
·
2013
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IF 1.918
Baocun Zhu,Jie Zhao,Haiqin Yu,Liangguo Yan,Qin Wei,Bin Du
Mercury (Hg) commonly found in the global environment poses a serious threat to the environment and human health because of its durability, easy transference and high biological accumulation. So, great attention has been paid to the quantification and removal of mercury. Herein, we present the synthesis and properties of a novel naphthalimide-functionalized magnetic fluorescent nanoparticle for simultaneous determination and removal of Hg 2+ . The proposed nanoparticle showed a good linear relation ( Y = −29.12216 X + 363.2597, correlation coefficient of R 2 = 0.9952) between the fluorescence intensity at 540 nm and the concentration of Hg 2+ varying from 0.1 μmol/L to 4.5 μmol/L. Additionally, our proposed nanoparticle exhibited excellent Hg 2+ -selectivity over other metal ions, which was ascribed to the formation of imide-Hg-imide complexes. The removal of Hg 2+ from contaminated water samples was achieved by the aggregation-induced sedimentation (AIS) strategy. Moreover, the suspended magnetic nanoparticle could be removed by external magnetic field, and the secondary pollution was avoided.
研究领域:
环境科学
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分析化学
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材料科学
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JOURNAL OF APPLIED POLYMER SCIENCE
·
2023
·Vol. 141
·期 4
·e54853
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IF 3
Meng Xie,Ke Yang,Yuye Wang,Xinjian Cheng
Heavy metal (HM) pollution is getting worse with the society progress. Identification and separation of HM ions are urgent issues in environmental protection and social development. In this work, 4-bromo-1,8-naphthalic anhydride was first reacted with amino-propylene glycol to synthesize naphthimide fluorophores bearing two hydroxyl groups. And then, –Br was substituents by 8-mercaptoquinoline, 2-mercaptoimidazole, and 4-mercaptopyridine, respectively. Eventually, it was reacted with 1,5-naphthalene diisocyanate (NDI) to synthesize fluorescent polyurethane (PU) films through the reaction between –OH and –NCO. It is found that the PU film not only has a specific identification ability to Hg 2+ /Hg + and Fe 3+ ions, but also can efficiently remove Hg 2+ /Hg + and Fe 3+ ions through adsorption. In addition, selective identification by fluorescence quenching was investigated. It is due to the photoinduced electron transfer mechanism (PET). Furthermore, the lowest detection limits for Fe 3+ , Hg 2+ , and Hg + were 6.0 × 10 −7 M, 4.7 × 10 −7 M, and 9.7 × 10 −7 M, respectively, which reflected the sensitivity of the probe and made it fluorescence probes. The three fluorescent small molecules were incorporated into PU, which not only retain their identification function, but also serve the purpose of adsorption and separation of HM ions.
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SENSORS AND ACTUATORS B-CHEMICAL
·
2023
·
IF 8.4
Panwang Hu,Jinjin Liu,Changkun Xia,Bangxiang Liu,Hengjia Zhu,Xiangheng Niu
Given heavy metals pose huge threats to ecological system and human health, it is significant to monitor them. With the favorable feature of catalytic signal amplification , redox-type nanozymes have been widely used to detect heavy metals. However, the presence of redox substances in samples can interfere with these catalytic reactions , impacting the accuracy and repeatability of detection. To avoid the situation, here we designed a non-redox nanozyme (3-mercaptopropionic acid modified nanoceria, MPA-CeO 2 ) and developed a novel approach free from matrix redox interference to detect Hg 2+ . The well-dispersed MPA-CeO 2 showed high and stable phosphatase-mimetic activity to catalyze the hydrolysis of colorless p -nitrophenyl phosphate ( p -NPP) to yellow p -nitrophenol ( p -NP). The addition of Hg 2+ could bind specifically onto the MPA-CeO 2 particles through S–Hg bond and cause the latter to aggregate, thereby suppressing the ability of MPA-CeO 2 with shielded active surfaces to hydrolyze p -NPP. According to the simple principle, highly sensitive and specific colorimetric measurement of Hg 2+ was achieved, and excellent reliability and practicability were demonstrated by real sample analysis. To our best knowledge, this study is the first one of detecting Hg 2+ using phosphatase-mimicking nanozymes, and it will inspire the exploration of new analytical methods via designing non-redox nanozymes for various targets.
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Journal of Materials Chemistry C
·
2021
·Vol. 9
·期 14
·4877-4887
·
IF 7.393
Li Xiao,Kaiqi Liu,Lian Duan,Xinjian Cheng
The detection and removal of heavy metals in the environment is urgent and meaningful. In this work, two types of fluorescent functionalized silks OSP and ASP have been prepared using worm silk as the substrate. These fluorescent silk probes exhibit an obvious fluorescence quenching effect in the presence of Hg2+ or Ag+, enabling silk to specifically detect Hg2+ or Ag+. An obvious color response occurs under visible light, from yellow to brown or gray, thereby realizing dual-channel identification of fluorescence and colorimetry. In addition, its sensing mechanism has been studied, and it is found that the probe reacts with metal ions as a reactive response. Compared with the fluorescent probes bearing one CC/CC bond, the probes with two terminal CC/CC bonds are more sensitive. And the excellent recognition ability can make the limit of detection as low as 0.25 μM. This indicates that silk fluorescent probes can be used to detect Hg2+ and/or Ag+.
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SPECTROCHIMICA ACTA PART A-MOLECULAR AND BIOMOLECULAR SPECTROSCOPY
·
2021
·
IF 4.098
Kaiyu Zhang,Yuxin Sang,Yingdi Gao,Qinxing Sun,Weina Li
In this paper, a simple and effective fluorescence turn-on approach for highly sensitive and selective monitoring Hg 2+ ions was designed by using carbon dots (CDs) and silver nanoparticles (AgNPs). It reveals that the fluorescence of CDs solution can be quenched in the presence of AgNPs through inner filter effect (IFE) and the quenched CDs-AgNPs system is turned on after addition of Hg 2+ ions, which is due to higher affinity of Hg 2+ and AgNPs than that of CDs and AgNPs, thus resulting the disappearance of AgNPs from the CDs-AgNPs composites and leading to the fluorescence turn-on of CDs. The developed fluorescence turn-on approach exhibited high selectivity and sensitivity for detection of Hg 2+ . Under the optimum experimental conditions, good linearity was achieved over the range of 100–160 μM and the limit of detection (LOD) was estimated to be 2.22×10 -8 M for Hg 2+ . The recoveries of Hg 2+ spiked in real samples ranged from 98.4% to 101.6%. Results of this study suggest that the fluorescence turn-on approach can be used to the detection of Hg 2+ in real water samples.
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DALTON TRANSACTIONS
·
2019
·Vol. 48
·期 48
·17800-17809
·
IF 4.052
Kan Li,Jing-jing Li,Ni Zhao,Ting-ting Xie,Bin Di,Li-li Xu
Hg2+ is highly toxic and hazardous and widely found in polluted water. To remove mercury ions from wastewater, there is an urgent need to investigate and develop new adsorbents. Herein, we synthesized three novel thioether-based metal–organic frameworks (MOFs) through a facile diffusion method or a solvothermal strategy, i.e. [(ZnCl2)3(L1)2·χ(solvent)]n (1), [(Cu2I3O2)4(CH4N0.5)4(L1)4(DMA)4·3(H2O)·χ(solvent)]n (2) and [(CuBr2)2(L2)2 CH3CN·χ(solvent)]n (3), where L1 = 1,3,5-tris((pyridin-4-ylthio)methyl)benzene and L2 = 2,4,6-trimethoxy-1,3,5-tris((pyridin-4-ylthio)methyl)benzene. The obtained thioether-based MOFs were characterized by single-crystal X-ray diffraction, Fourier transform infrared spectroscopy, elemental analysis and thermogravimetric analysis. Further studies revealed that they could remove Hg2+ from water. They have high adsorptivity (up to 362 mg g−1) and are highly efficient in removing Hg2+ (up to 95%). Besides, these MOFs can be recycled and can selectively remove Hg2+ from water in the presence of other metal ions. Consequently, these MOFs are highly promising candidates for the selective absorption and removal of mercury ions from water.
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NANO
·
2018
·
IF 1.1
Jing Liu,Xiao Li,Xin Gong,Taiping Qing,Peng Zhang,Bo Feng
The incorporation of dopant atoms alters the structure of MoS 2 , resulting in unique properties and practical applications. Herein, a facile and low-cost approach for producing N-doped molybdenum disulfide (N-MoS 2 ) was carried out using melamine and MoS 2 in a laboratory tube furnace at 400 ∘ C for 2 h. The N-MoS 2 fluorescent probe showed sensitive response to Hg 2 + in a wide concentration range of 0.4–10 μ M with a low limit of 28 nM. The practical applicability of detecting Hg 2 + in lake water samples may open a new way to evaluate Hg 2 + in wastewater.
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