Frontiers in Pharmacology
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2019
·
IF 3.845
Luo Zhuo,Liu Li-Fang,Wang Xiao-Hua,Li Wen,Jie Chong,Chen Huan,Wei Fan-Qin,Lu Dan-Hua,Yan Chang-Yu,Liu Bo,Kurihara Hiroshi,Li Yi-Fang,He Rong-Rong
Stress has been proven to modulate an individual’s immune system through the release of pituitary and adrenal hormones such as the catecholamines, growth hormone, and glucocorticoids. These signal molecules can significantly alter the host immune system and make it susceptible to viral infection. In this study, we investigate whether epigoitrin, a natural alkaloid from Isatis indigotica, provides protection against influenza infection by reducing the host’s susceptibility to influenza virus under stress and its underlying mechanism. To support it, the mouse restraint stress model and the corticosterone-induced stress model were employed. Our results demonstrated that epigoitrin significantly decreased the susceptibility of restraint mice to influenza virus, evidenced by lowered mortality, attenuated inflammation, and decreased viral replications in lungs. Further results revealed that epigoitrin reduced the protein expression of mitofusin-2 (MFN2), which elevated mitochondria antiviral signaling (MAVS) protein expression and subsequently increased the production of IFN-β and interferon inducible transmembrane 3 (IFITM3), thereby helping to fight viral infections. In conclusion, our study indicated that epigoitrin could reduce the susceptibility to influenza virus via mitochondrial antiviral signaling.
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高影响因子
CELL CALCIUM
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2018
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IF 3.718
Bin Liu,Wanxin Cao,Jiping Li,Jun Liu
Adenosine triphosphate (ATP) is stored as lysosomal vesicles in marginal cells of the stria vascular in neonatal rats, but the mechanisms of ATP release are unclear. Primary cultures of marginal cells from 1-day-old Sprague–Dawley rats were established. P2Y 2 receptor and inositol 1,4,5-trisphosphate (IP3) receptor were immunolabelled in marginal cells of the stria vascular. We found that 30 μM ATP and 30 μM uridine triphosphate (UTP) evoked comparable significant increases in the intracellular Ca 2+ concentration ([Ca 2+ ] i ) in the absence of extracellular Ca 2+ , whereas the response was suppressed by 100 μM suramin, 10 μM 1-(6-(17β-3-methoxyester-1,3,5(10)-trien-17-yl)amino)-hexyl)-1H-pyrrole-2,5-dione(U-73122), 100 μM 2-aminoethoxydiphenyl borate (2-APB) and 5 μM thapsigargin (TG), thus indicating that ATP coupled with the P2Y 2 R-PLC-IP3 pathway to evoke Ca 2+ release from the endoplasmic reticulum (ER). Incubation with 200 μM Gly-Phe-β-naphthylamide (GPN) selectively disrupted lysosomes and caused significant increases in [Ca 2+ ] I ; this effect was partly inhibited by P2Y 2 R-PLC-IP3 pathway antagonists. After pre-treatment with 5 μM TG, [Ca 2+ ] i was significantly lower than that after treatment with P2Y 2 R-PLC-IP3 pathway antagonists under the same conditions, thus indicating that lysosomal Ca 2+ triggers Ca 2+ release from ER Ca 2+ stores. Baseline [Ca 2+ ] i declined after treatment with the Ca 2+ chelator 50 μM bis-(aminophenolxy) ethane- N , N , N ʹ, N ʹ-tetra-acetic acid acetoxyme-thyl ester (BAPTA-AM) and 4 IU/ml apyrase. 30 μM ATP decrease of the number of quinacrine-positive vesicles via lysosome exocytosis, whereas the number of lysosomes did not change. However, lysosome exocytosis was significantly suppressed by pre-treatment with 5 μM vacuolin-1. Release of ATP and β-hexosaminidase both increased after treatment with 200 μM GPN and 5 μM TG, but decreased after incubation with 50 μM BAPTA-AM, 4 IU/ml apyrase and 5 μM vacuolin-1. We suggest that ATP triggers Ca 2+ release from the ER, thereby contributing to secretion of lysosomal ATP via lysosomal exocytosis. Lysosomal stored Ca 2+ triggers Ca 2+ release from the ER directly though the IP3 receptors, and lysosomal ATP evokes Ca 2+ signals indirectly via the P2Y 2 R-PLC-IP3 pathway.
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JOURNAL OF CHROMATOGRAPHY B-ANALYTICAL TECHNOLOGIES IN THE BIOMEDICAL AND LIFE SCIENCES
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2019
·
IF 2.813
Yang Li,Ying Peng,Ping Ma,Mengyue Wang,Chongsheng Peng,Pengfei Tu,Xiaobo Li
Cistanche tubulosa , one species of Cistanches Herba, was recently confirmed to have antidepressant efficacy in chronic unpredictable stress (CUS) rats by restoring homeostasis of intestinal microbiota . In this paper, we aim to explore the metabolic profile of C. tubulosa in normal and CUS induced depressive model rats in vitro and in vivo . Using UPLC-Q-TOF-MS, the in vitro gastrointestinal metabolism of Cistanche tubulosa extract (CTE) was evaluated in both normal and CUS rats. At the same time, in vivo metabolism of CTE in normal and depressed rats were also investigated in rat urine and feces. A total of 20 and 26 metabolites were characterized from in vitro and in vivo metabolism in normal and CUS rats, respectively. CTE was metabolized to aglycones and degradation products of phenylethanoid glycosides (PhGs) and iridoid glycosides whether by normal or depressed rat intestinal microbiota in vitro . Phase II metabolites of aglycones and degradation products of PhGs and iridoid glycosides were the main metabolites in rat urine and feces. Additionally, the metabolic capability to generate secondary glycosides and aglycones in depressive rat intestinal microbiota was much weaker than that in normal rat intestinal microbiota, which was attributed to the disordered glycoside hydrolases produced by intestinal microbiota in CUS depressed rats. The results of this study laid the foundation for understanding the metabolic process and therapeutic mechanism of CTE's antidepressant property.
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Frontiers in Nutrition
·
2023
·
IF 5
Cao Feiwei,Ding Qinchao,Zhuge Hui,Lai Shanglei,Chang Kaixin,Le Chunyan,Yang Guorong,Valencak Teresa G.,Li Songtao,Ren Daxi
This present study was designed to explore the protective role of Lactobacillus plantarum ZJUIDS14 against Non-alcoholic Fatty Liver Disease (NAFLD) in a high-fat-diet (HFD)-induced C57BL/6 mice model. The probiotic (109 CFU/every other day) was administered by oral gavage for 12 weeks. We found that L. plantarum ZJUIDS14 intervention significantly alleviated HFD related hepatic steatosis, liver damage, insulin resistance, and increased hepatic expression of peroxisome proliferator activated receptor α (PPAR-α) while stimulating the activation of AMP-activated protein kinase (AMPK). Furthermore, L. plantarum ZJUIDS14 improved mitochondrial function as reflected by an increase in dynamin related protein 1 (DRP1) and a decrease of proteins associated with oxidative phosphorylation (OXPHOS) after the treatment. Additionally, mice from the L. plantarum ZJUIDS14 group had a restored intestinal flora and homeostasis involving Coprostanoligenes group, Ruminococcaceae UCG-014, Allobaculum, Ruminiclostridium 1, and Roseburia. Meanwhile, these five genera exhibited a significant (negative or positive) association with ileum inflammation mRNA levels and SCFA contents, by Spearman’s correlation analysis. In general, our data demonstrated that L. plantarum ZJUIDS14 mitigates hepatic steatosis and liver damage induced by HFD. Specifically, they strengthened the integrity of the intestinal barrier, regulated gut microbiota, and improved mitochondrial function. Our data provide an experimental basis for L. plantarum ZJUIDS14 as a promising candidate to prevent NAFLD.
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BIO-MEDICAL MATERIALS AND ENGINEERING
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2022
·Vol. 33
·期 1
·77-90
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IF 1.234
Chang Yili,Zhang Fubao,Liu Feng,Shi Lianshui,Zhang Lin,Zhu Hongshui
BACKGROUND:Craniofacial soft-tissue defects mostly have an impact on the treatment of various oral diseases. Tissue expander is an important technique for tissue reconstruction, especially for soft tissues in reconstructive surgery. OBJECTIVE:This research aimed to develop a new self-swelling tissue expander, namely hydrogel, for soft tissue reconstruction in craniofacial region. METHODS:In vitro, the chemical and physical characteristics of hydrogel were evaluated by SEM, swelling rate, mechanical testing, EDS, and FT-IR. In vivo, the craniofacial implant model of SD rats were divided into group A as control, group B with hydrogels for 1 week expansion, group C for 2 weeks and group D for 4 weeks (n = 5), and the effects were analyzed by HE staining, histological and radiographic evaluation. RESULTS:The in vitro results suggested that dry hydrogel possessed a uniform surface with micropores, the surface of post-swelling hydrogel formed three-dimensional meshwork. Within 24 hours, hydrogels expanded markedly, then slowed down. The mechanical property of hydrogels with longer expansion was better, whose main elements were carbon and oxygen. FT-IR also verified its molecular structure. In vivo, the wounds of rats recovered well, hydrogels could be removed as one whole piece with original shape and examined by radiographic evaluation, besides, the expanded skin and developed fibrous capsule formed surrounding hydrogels. CONCLUSION:The new expander was designed successfully with good chemical and physical characteristics, and could be applied in an animal model to help tissue reconstruction.
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JOURNAL OF PHARMACEUTICAL SCIENCES
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2022
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IF 3.784
Luyu Bai,Fang Lei,Rui Luo,Qingsong Fei,Zhiyun Zheng,Ning He,Shuangying Gui
The purpose of this study was to develop a thermosensitive in situ gel delivery system based on Poloxamer 407 and Poloxamer 188 for ocular administration of vancomycin to treat systemic diseases . The vancomycin thermosensitive in situ gel was characterized using differential scanning calorimetry , rheological and drug release analyses. Additionally, pharmacokinetic studies and irritation tests of the gel were conducted after ocular administration in rabbits. The gel maintained a flowing liquid state under non-physiological conditions (25°C) to facilitate administration, and it transformed into a semi-solid state under physiological conditions (dilution with tears, 34°C), which prolonged its retention time in the eye. The gel erosion and drug release tests showed an excellent linear relationship between the cumulative drug release rate and the cumulative gel erosion rate, indicating a zero-order kinetic process. The pharmacokinetic analyses showed that the peak concentration, area under the curve, and bioavailability of the vancomycin thermosensitive in situ gel were 1.44, 1.98 and 1.93 times greater, respectively, that the values of vancomycin eye drops. Therefore, thermosensitive in situ gel may serve as a drug delivery system that can overcome the limitations of existing formulations of small-molecule peptides.
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Journal of Pharmaceutical Investigation
·
2022
·Vol. 52
·期 5
·639-648
·
IF 0
Lei Fang,Zhang Huimin,Luo Rui,Fei Qingsong,Bai Luyu,He Ning
Purpose The present study was designed to formulate a thermoreversible in situ gel system for sustained ocular delivery of desmopressin acetate (DDAVP) for the treatment of polyuria. Methods The cold method was employed for the preparation of in situ gel formulations using different levels of poloxamer 407 (F-127) and carboxymethyl chitosan (CMCTS). The optimal formulation was selected based on gelation temperature, rheological behavior, and in vitro drug release, and further evaluated by thermal, ocular irritation, residence time, in vivo pharmacokinetics, and pharmacodynamic analyses. Results The optimal formulation, composed of F-127 (20%) and CMCTS (1.5%), showed appropriate fluidity at room temperature (25 °C) and transitioned to semisolid gels at physiological temperature (34 °C). The optimal gels showed pseudoplastic fluid properties and favorable thixotropy characteristics in rheological tests. Differential scanning calorimetry analysis revealed even dispersal of DDAVP in the matrix of gels. Results from in vivo studies demonstrated that the newly prepared gel successfully prolonged the residence time of DDAVP, improved ocular bioavailability, and exerted the same antidiuretic effect as ophthalmic drops. Conclusion The developed thermoreversible ophthalmic in situ gel of DDAVP presents a promising formulation for the treatment of polyuria.
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Materials Research Express
·
2021
·Vol. 8
·期 8
·085002
·
IF 1.62
Xiangming Liu,Xiaoshi Peng,Tao Xu,Yulong Li,Huiyue Wei,Zanyang Guan,Feng Wang
Ternary Ag-In-S and quaternary Ag-In-Zn-S nanoparticles with different ratio of Ag/In/Zn/S are synthesized. The incorporation of Zn into Ag-In-S nanoparticles leads to the increase in the optical bandgap and the blue shift of photoluminescence (PL). The optical properties of these nanoparticles are significantly dependent on the chemical composition of nanoparticles. Time-resolved PL spectroscopy in nanosecond time regime is used to study the recombination processes of carriers, which involve the surface states and intrinsic crystallographic defects. These measurements support the donor-acceptor model, in which the PL is achieved by radiative recombination of the localized electron and hole.
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Journal of Cleaner Production
·
2021
·
IF 9.297
Li Ma,Yuhong Li,Lei Yao,Hongmei Du
Previous studies on the distribution patterns and health risks of polycyclic aromatic hydrocarbons (PAHs) from cities were focused on soils, leaving the soil-plant system less investigated. In this study, PAHs in the soil-turfgrass system in the urban area of the megacity Shanghai were investigated. The results indicated that total PAH concentrations in soils and turfgrasses ranged from 163.02 to 14,289.30 ng g −1 and 45.15 to 1076.98 ng g −1 , respectively. On average, four- and three-ring PAHs such as fluoranthene (14.4%) and pyrene (12.4%) were dominant in soils and naphthalene (37.1%) was especially enriched in turfgrasses. The significant differences in compositional profiles of PAHs between soils and turfgrasses implied selective uptake and translocation of PAHs by turfgrasses from soils. The in situ bioconcentration factors (BCFs) of ∑ 16 PAHs in turfgrasses ranged 0.004–1.3, with ryegrass the highest. BCFs decreased significantly with increasing PAH concentrations in soils, suggesting that turfgrass-assisted PAH removal vial root uptake may be more efficient in slightly contaminated soils. Source appointment results indicated that PAHs in Shanghai were majorly derived from fossil fuel/biomass combustion, vehicular emissions and coking. The incremental lifetime cancer risk (ILCR) analysis generally showed a potential cancer risk for adults (total ILCRs > 1 × 10 −6 for all sites) and children (total ILCRs > 1 × 10 −6 for most sites) especially through dermal contact with soil. Some sites showed total ILCR values above 1 × 10 −4 , indicating high health risks for both. The results are informative for contamination prevention and control in urban soils from Shanghai and will provide guidance for further exploration of the phytoremediation potential of turfgrasses.
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Communications Materials
·
2025
·Vol. 6
·期 1
·233
·
IF 9.6
Wang Ruoming,Yang Guangping,Yang Tianxiang,Lu Yuzheng,Raza Rizwan,Zhu Bin,Lund Peter D.,Yun Sining
High ionic conductivity at reduced temperatures may allow electrochemical cell applications to be efficiently scaled up. Herein, we introduce an approach that exploits electrode-electrode synergy and in situ structural transformation to increase the ionic conductivity of an electrolyte within the electrochemical cell. This transformation is based on the formation of a Li 2 TiO 3 -Li 2 CO 3 heterostructure electrolyte via the combination of a TiO 2 semiconductor precursor, polystyrene spheres as the soft skeleton, and the lithium-based electrode LiNi 0.8 Co 0.15 Al 0.05 O 2 . The unique surface and interface of the heterostructure electrolyte enable the formation of long-range ion transport channels that lower electrode impedance, resulting in a superior ionic conductivity of 0.23 S cm −1 at 550 °C and a high-power density of 1239 mW cm −2 . Our general method to form superionic electrolytes shows potential in fuel cell technologies.
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STEROIDS
·
2019
·
IF 2.136
Shu-Qi Dong,Shuang-Shuang Wang,Ji-Xiao Zhu,Rong-Hao Mu,Cheng-Fu Li,Di Geng,Qing Liu,Li-Tao Yi
Our previous study has demonstrated that oleanolic acid produced an antidepressant-like effect in mice exposed to chronic stress. Considering that serine/threonine-protein kinase 1 (SGK1) is involved in stress response, the present study aimed to evaluate the involvement of SGK1 in the antidepressant-like effects of oleanolic acid in depression-like mice induced by long term corticosterone (CORT) injection. Behaviors, SGK1, brain-derived neurotrophic factor (BDNF) and its downstream targets were assessed after administration with oleanolic for three weeks. The results indicated that oleanolic acid increased the sucrose preference and decreased the immobility time. In addition, oleanolic acid decreased SGK1 and activated BDNF-AKT/mTOR signaling in the hippocampus of CORT-induced animals. However, we found that GSK650394, an inhibitor of SGK1 did not exert any effects on the behaviors, GR levels and BDNF signaling. The number of spines in hippocampal neurons was not changed by GSK650394 as well. Taken together, this study demonstrated that oleanolic acid produced the antidepressant-like effects, which might be related to the down-regulation of SGK1. However, inhibition of SGK1 directly lacks the effects in the treatment of depression.
研究领域:
药理学
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神经科学
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精神病学
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MOLECULAR REPRODUCTION AND DEVELOPMENT
·
2021
·Vol. 88
·期 6
·405-415
·
IF 2.609
Li Ren,Yanwen Zhang,Yining Xin,Guo Chen,Xiaoxiao Sun,Yingqi Chen,Bin He
The effect of stress on male fertility is a widespread public health issue, but less is known about the related signaling pathway. To investigate this, we established a hypercortisolism mouse model by supplementing the drinking water with corticosterone for four weeks. In the hypercortisolism mice, the serum corticosterone was much higher than in the control, and serum testosterone was significantly decreased. Moreover, corticosterone treatment induced decrease of sperm counts and increase of teratozoospermia. Increased numbers of multinucleated giant cells and apoptotic germ cells as well as downregulated meiotic markers suggested that corticosterone induced impaired spermatogenesis. Further, upregulation of macrophage-specific marker antigen F4/80 as well as inflammation-related genes suggested that corticosterone induced inflammation in the testis. Lactate content was found to be decreased in the testis and Sertoli cells after corticosterone treatment, and lactate metabolism-related genes were downregulated. In vitro phagocytosis assays showed that the phagocytic activity in corticosterone-treated Sertoli cells was downregulated and accompanied by decreased mitochondrial membrane potential, while pyruvate dehydrogenase kinase-4 inhibitor supplementation restored this process. Taken together, our results demonstrated that dysfunctional phagocytosis capacity and lactate metabolism in Sertoli cells participates in corticosterone-induced impairment of spermatogenesis.
研究领域:
内分泌学
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生殖生物学
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免疫学
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分子生物学
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