JOURNAL OF HAZARDOUS MATERIALS
·
2025
·
IF 11.3
Zhenyong Dong,Feixiang Zheng,Hao Wang,Yukai Liu,Xiao Ren,Feng Lin,Song Zhou,Li Chen,Yan-qing Lu,Guanghui Wang
The use of multiple pesticides in agriculture has caused mixed residues, complicating food safety testing. Surface-enhanced Raman scattering (SERS) offers high sensitivity and fingerprinting capabilities for pesticide detection; however,its application is limited by spot size, uneven sample distribution, spectral analysis challenges in mixed samples, and the lack of integrated automated systems for sample preparation, detection, and analysis. Hence, we developed a SERS microfluidic chip integrated with plasmonic microsphere lens arrays, which integrates optical field-modulated microspheres and plasmonic nanostructures to realise the transition of SERS detection from point-hotspots to uniform area-hotspots, achieving high-sensitivity, high-stability Raman detection under wide-field laser excitation. Moreover, a comprehensive a sample-to-answer total analysis system was established with this chip as the core, incorporating the micro-QuEChERS pretreatment module for efficient extraction of pesticides in complex matrices and the random forest-dual annealing spectral parsing algorithm for mixed spectral analysis. The results indicate that the limits of detection for 2,4-dichlorophenoxyacetic acid, acetamiprid, and thiabendazole were 1.15 nM, 0.63 nM, and 0.69 nM, respectively. The linear ranges were 10⁻⁴–10⁻⁹ M, with R² values of 0.9982, 0.9989, and 0.9996, respectively. Average recoveries ranged from 90.8 % -104.66 %, 89.1 %-107.89 %, and 89.73 %-108.4 %, respectively, with relative standard deviations below 8 %. The algorithm quantified mixed pesticides with mean absolute percentage errors of 4.96 %, 5.35 %, and 7.72 %, respectively. The system showed 3 s detection response and 15-minute analysis time. Thus, the method provides a rapid, on-site solution for detecting mixed pesticides in complex matrices by exceptional optical performance and automated processing functionality.
✓ 已验证
产品关联
DOI
PubMed
高影响因子
JOURNAL OF NANOBIOTECHNOLOGY
·
2025
·Vol. 23
·期 1
·791
·
IF 12.6
Qiao Wenyu,Yang Luling,Liu Yu,Zhang Huaiyuan,Zheng Zhihuan,Gu Tianheng,Zhu Jiaxi,Hu Xinyi,Li Gangze,Zhang Yuxia,Xiao Meng,Zhang Lin
Excessive accumulation of reactive oxygen species (ROS) and dysbiosis of the gut microbiota are pivotal contributors to the pathogenesis of inflammatory bowel disease (IBD) and associated fibrosis. In this study, a microfluidic approach was utilized to fabricate a multifunctional therapeutic microsphere system by encapsulating hollow mesoporous cerium oxide nanoparticles (HCeO 2 ) loaded with KP1 short peptides-specific inhibitors of the TGF-β/Smad signaling pathway. The resulting composite formulation (KP1@HCeO₂@SAM) was designed to achieve synergistic antioxidant, anti-inflammatory, and antifibrotic effects. The developed microspheres exhibited prolonged intestinal retention and effectively modulated gut microbiota composition, increasing the relative abundance of probiotic species by over sevenfold. Moreover, they demonstrated remarkable reactive oxygen species (ROS) scavenging efficiency (> 95%) and significant inhibition of fibrotic signaling cascades. In dextran sulfate sodium (DSS)-induced murine colitis models, treatment with the microspheres led to a substantial reduction in Disease Activity Index (DAI) (> 76%), restoration of intestinal barrier integrity (> 90%), and mitigation of fibrotic progression, as indicated by a 66% decrease in α-SMA expression. These findings establish a novel and integrative therapeutic platform for the effective management of IBD. Graphical abstract
✓ 已验证
产品关联
DOI
PubMed
高影响因子
CARBOHYDRATE POLYMERS
·
2018
·
IF 5.158
Wei Li,Li Zhang,Qi Li,Shenggao Wang,Xiaogang Luo,Hongbing Deng,Shilin Liu
In order to develop a biosensor based on porous structured cellulose microspheres for glucose detection with “signal-and-color” output, in this work, active group carboxyl was introduced to cellulose matrix by using plasma technology, and then glucose oxidase (GOx) was chemically immobilized through EDC-NHS cross-linking reaction. The cellulose microgels containing 21.28 mg/g of enzymes exhibited a fast response to 0.003 M glucose within only 4 min. As for detecting subject with a lower concentration of glucose, the probe still worked. When the concentration of glucose solution was 0.005 M, it took only 2 min that the reaction mixture changed from colorless to yellow. By the introduction of starch, the reaction mixture presented as amaranth color. Besides, the porous-structured substrate and the facile plasma technology were also promising for constructing enzyme-driven catalytic systems with enhanced performance.
研究领域:
生物传感
·
材料科学
·
生物化学
✓ 已验证
DOI
PubMed
高影响因子
APPLIED SURFACE SCIENCE
·
2020
·
IF 6.182
Wenxiu Yang,Zhiqing Ge,Guoqing Xu,Chu Zhao,Mozhen Wang,Shengchi Zhuo,Xuewu Ge
The synthesis and stable loading of metal nanoclusters have always been a hot and difficult topic in the field of nano-metal catalysts. In this work, micron-sized inverse opal SiO 2 microspheres (IO-SiO 2 ) are firstly prepared as the carrier of platinum nanoclusters (PtNCs) through a sol-gel process of tetraethyl orthosilicate (TEOS) on sacrificed polystyrene (PS) colloidal crystal microspheres template. After the IO-SiO 2 carriers are dispersed into the aqueous solution of H 2 PtCl 6 and irradiated by 60 Co γ-ray radiation for a certain absorbed dose in a deoxygenized atmosphere, PtNCs with a size of less than 2 nm are in situ synthesized based on a redox equilibrium of Pt(0)/Pt(IV) induced by effect of the reductive species (e aq − , H ) and oxidizing radicals (HO ) produced by the radiolysis of water, and immobilized on IO-SiO 2 without the help of any stabilizer. Due to the large pore volume of the IO-SiO 2 , the loading capacity of PtNCs can reach as high as 18.63 μg/mg microsphere . The prepared IO-SiO 2 -supported PtNCs (PtNCs@IO-SiO 2 ) show excellent catalytic activity and recyclability for the reduction of o-nitroaniline by NaBH 4 as the model reaction. This work offers a new route to prepare stable and high-performance metal nanocluster catalysts loaded on porous microspheres using γ-ray radiation technique.
研究领域:
材料科学
·
催化化学
✓ 已验证
DOI
高影响因子
Regenerative Biomaterials
·
2020
·Vol. 7
·期 4
·381-390
·
IF 4.882
Liu Zhu,Ye Weilong,Zheng Jingchuan,Wang Qindong,Ma Guowu,Liu Huiying,Wang Xiumei
Sequential administration and controlled release of different drugs are of vital importance for regulating cellular behaviors and tissue regeneration, which usually demands appropriate carriers like microspheres (MS) to control drugs releases. Electrospray has been proven an effective technique to prepare MS with uniform particle size and high drug-loading rate. In this study, we applied electrospray to simply and hierarchically fabricate sphere-in-sphere composite microspheres, with smaller poly(lactic-co-glycolic acid) MS (∼8–10 μm in diameter) embedded in a larger chitosan MS (∼250–300 μm in diameter). The scanning electron microscopy images revealed highly uniform MS that can be accurately controlled by adjusting the nozzle diameter or voltage. Two kinds of model drugs, bovine serum albumin and chlorhexidine acetate, were encapsulated in the microspheres. The fluorescence-labeled rhodamine-fluoresceine isothiocyanate (Rho-FITC) and ultraviolet (UV) spectrophotometry results suggested that loaded drugs got excellent distribution in microspheres, as well as sustained, slow release in vitro. In addition, far-UV circular dichroism and matrix-assisted laser desorption/ionization time-of-flight mass spectrometry (MALDI-TOF-MS) results indicated original secondary structure and molecular weight of drugs after electrospraying. Generally speaking, our research proposed a modified hierarchically electrospraying technique to prepare sphere-in-sphere composite MS with two different drugs loaded, which could be applied in sequential, multi-modality therapy.
研究领域:
生物材料学
·
药物递送系统
·
组织工程
✓ 已验证
DOI
高影响因子
CHEMICAL ENGINEERING JOURNAL
·
2022
·
IF 16.744
Liang Wu,Yichang Xu,Kun Xi,Yong Gu,Jincheng Tang,Tianwen Xin,Huilin Yang,Lingjun Wang,Wenguo Cui,Liang Chen
The ‘clean-to-repair’ rhythm in the dynamic pathological osteoimmune microenvironment is essential to bone healing but often disturbed by the intense inflammation. Inspired by the native osteoimmune microenvironment, we presented an injectable porous microsphere featured hierarchical micro/nano-structure via an ingenious integration of microfluidic microspheres and self-assembly collagen nanofibers, which could mimic the extracellular matrix and activate the integrin-mediated macrophage (Mφ) polarization, so as to realize a paracrine-transformed microenvironment reprogramming. The integrated hierarchical structure endowed with multiscale fibrous topology and optimized chemical cues well balanced the contradiction between large pores and reduced motifs. In vitro , owing to the modulation of integrin-mediated M2Mφ polarization and its paracrine secretion of endogenous cytokines, such as VEGF, PDGF-BB, TGF-β1, and BMP-2, the microsphere not only supported the tissue repair cell growth, but also promoted angiogenesis and mineralization by 2 and 3 folds, respectively. The capabilities of the osteoimmuno-reprogramming microsphere in progressive vascular sprouting maturation and in situ osteoinduction and osseointegration were further verified in a rat femoral condylar defect model. Hence, the so-produced hierarchical structured microsphere could manipulate the osteoimmune microenvironment by M2Mφ polarization and reconstruct the bone defects.
研究领域:
生物医学工程
·
组织工程
·
免疫学
·
骨再生
✓ 已验证
DOI
高影响因子
JOURNAL OF NANOBIOTECHNOLOGY
·
2024
·Vol. 22
·期 1
·1-18
·
IF 10.6
Jin Jiale,Xia Xiaowei,Ruan Chengxin,Luo Zhiyuan,Yang Yiqi,Wang Dongyu,Qin Yifang,Li Dongdong,Zhang Yong,Hu Yihe,Lei Pengfei
Macrophage metabolism dysregulation, which is exacerbated by persistent stimulation in infectious and inflammatory diseases, such as diabetic infectious bone defects (DIBD), eventually leads to the failure of bone repair. Here, we have developed an injectable, macrophage-modulated GAPDH-Silence drug delivery system. This microsphere comprises chondroitin sulfate methacrylate (CM) and methacrylated gelatin (GM), while the dimethyl fumarate (DMF)-loaded liposome (D-lip) is encapsulated within the microsphere (CM@GM), named D-lip/CM@GM. Triggered by the over-expressed collagenase in DIBD, the microspheres degrade and release the encapsulated D-lip. D-lip could modulate metabolism by inhibiting GAPDH, which suppresses the over-activation of glycolysis, thus preventing the inflammatory response of macrophages in vitro. While beneficial for macrophages, D-lip/CM@GM is harmful to bacteria. GAPDH, while crucial for glycolysis of staphylococcal species ( S. aureus ), can be effectively countered by D-lip/CM@GM. We are utilizing existing drugs in innovative ways to target central metabolism for effective eradication of bacteria. In the DIBD model, our results confirmed that the D-lip/CM@GM enhanced bacteria clearance and reprogrammed dysregulated metabolism, thereby significantly improving bone regeneration. In conclusion, this GAPDH-Silence microsphere system may provide a viable strategy to promote diabetic infection bone regeneration.
研究领域:
生物医学工程
·
药剂学
·
免疫学
·
传染病学
·
组织工程
✓ 已验证
产品关联
DOI
PubMed
高影响因子
Regenerative Biomaterials
·
2024
·
IF 5.6
Zhou Xiaosong,Guo Min,Wang Zongliang,Wang Yu,Zhang Peibiao
Bioactive microspheres coated with acellular matrix (aECM) have received extensive attention in bone tissue engineering. In this work, biomimetic microspheres with different aECM ratios, uniform size and controllable size were prepared easily by blending natural porcine dermal aECM and poly (lactic-co-glycolic acid) (PLGA) using electrohydrodynamic spraying and solidification actuated by solvent extraction (ESASE) method. In this work, the appropriate polymer concentration and preparation voltage were investigated, and the surface morphology of the microspheres were observed by scanning electron microscope. Sirius red was used to visualize aECM exposure on the surface of the microspheres. The in vitro and in vivo experiments was carried out to evaluate the bioactivity and osteogenic properties of the microspheres. The results showed that the morphology and size of PLGA microspheres had little influence on the aECM blending. In vitro experiments showed that the higher the content of aECM, the better the cell adhesion performance. In vivo, rat calvarial defect models were observed and characterized at 4 and 8 weeks postoperatively, and the values of BV/TV of 50aECM/PLGA were 47.57 ± 1.14% and 72.92 ± 2.19%, respectively. The results showed that the skull healing effect was better in aECM-containing microspheres. In conclusion, aECM/PLGA composite microspheres can increase cell adhesion performance through the addition of aECM. Moreover, in vivo experiments have proved that aECM/PLGA microspheres is beneficial to bone repair, which means the aECM/PLGA microspheres is a promising bone tissue engineering material.
研究领域:
组织工程
·
生物材料
·
生物医学工程
·
再生医学
✓ 已验证
产品关联
DOI
PubMed
高影响因子
JOURNAL OF MOLECULAR LIQUIDS
·
2025
·
IF 5.2
Xiujun Wang,Zhe Sun,Jian Zhang,Shenwen Fang,Jiaqi Huang
Polymer microspheres have been used in the field of heterogeneous reservoir profile control, but their temperature and salt resistance still need to be improved. In this paper, N’N-dimethylacrylamide (DMA) and sodium styrene sulfonate (NaSS) were used as monomers, divinylbenzene (DVB) was used as cross-linker, and inverse emulsion polymerization was adopted to preparation the ultra-high temperature resistant polymer microsphere. After the investigation of the influences of emulsification shear time, monomer concentration, mass ratio of DMA to NaSS, and DVB addition on the polymerization result, a kind of polymer microspheres (PNS microspheres) that could maintain long-term stability in different brines (including seawater) at 150 °C for 45 days was prepared. The temperature and salt resistance of PNS microspheres are significantly better than that of polyacrylamide microspheres. The size of PNS microspheres is about 30 μm, and they can expand by about 2–4 times in low salinity brine at 150 °C, while they do not expand in seawater. Plugging effect experiment results showed that PNS microspheres can achieve a plugging rate of over 90 % for porous media with a water permeability ≤ 600 mD. T 2 spectrum of PNS microsphere displacement effect presented that it can effectively block the large pore throats and achieve micro fluid flow diversion, while oil displacement effect showed it can improve oil recovery of about 20 %. This work provides a viable candidate material expected to be applied for profile control in offshore high-temperature heterogeneous reservoirs.
研究领域:
石油工程
·
材料科学
✓ 已验证
产品关联
DOI
高影响因子
Advanced Materials Technologies
·
2026
·
IF 6
Lianjia Zhao,Chaoyuan Fan,Kunyuan Gao,Zhuangzhuang Liao,Wei Gao,Xiaohuan Li,Yongyao Chen
Underwater impacts are a leading cause of numerous accidents, and pressure sensors are essential for detecting them. However, the inherent characteristics of existing rigid pressure sensors make it difficult to effectively detect impacts. Here, we fabricate 3D layered polystyrene/MXene nanocomposite films by incorporating polystyrene microspheres into MXene nanoflakes, which exhibit outstanding flexible pressure-sensing performance. Finite element analysis indicates that the presence of polystyrene microspheres modulates the pressure-sensing pathway of nanocomposite films by adjusting stress distribution. The sensitivity of the sensor based on this nanocomposite film has been improved by 4005.67% compared to pristine MXene films. Meanwhile, it maintains stable performance even under bending conditions, with sensitivity decreasing by only 6.1%. Ultimately, the sensing array system based on this sensor achieves effective detection of simulated underwater impacts. This work will introduce a novel approach to developing flexible sensing materials and arrays for effectively detecting underwater impacts.
✓ 已验证
DOI
高影响因子
ADVANCED MATERIALS
·
2026
·
IF 29.1
Zhisheng Xiao,Zhiqiang Wu,Qiaofeng Li,Jiafei Zhu,Bo Liu,Yu Miao,Yifan Yang,Junjie Zhu,Linfu Chen,Boxiong Bai,Feng Pan,Yang Yang,Qian Chen
Inhalable mucosal vaccines elicit mucosal immunity non-invasively but are hindered by lung barriers like mucociliary clearance and phagocytosis, which typically necessitate multiple doses. Herein, we developed an innovative inhalable porous microsphere (pMS) vaccine using a single Food and Drug Administration-approved material, featuring a dual-scale design: an aerodynamic diameter of 4.84 µm for optimal deep lung deposition and a geometric size of 14.7 µm to evade phagocytosis for long-term retention. Notably, respiratory motion facilitates the penetration of pMS through the mucus layer into the pulmonary interstitium, where it gradually releases antigens and adjuvants. Remarkably, a single inhalation induced durable immunity, sustaining high levels of IgG and IgA for one year, alongside enhancing tissue-resident memory T cells in the lung and promoting germinal center expansion in the lymph node. This provided long-term protective efficacy, significantly inhibiting lung tumor metastasis even a year after inhalation. Beyond prophylaxis, this vaccine demonstrated remarkable therapeutic efficacy across multiple preclinical models, including the in situ lung tumor model, postoperative recurrence prevention model, and clinically relevant Patient-Derived tumor Xenograft (PDX) model. The dry powder pMS platform is scalable, stable, and clinically translatable, emerging as a versatile therapeutic strategy to adapt to diverse Lung-related diseases.
✓ 已验证
产品关联
DOI
PubMed
高影响因子
ACS Applied Energy Materials
·
2026
·
IF 5.5
Chengbo Huang,Yixing Yu,Hupeng Xu,Yuanjin He,Hailang Chen,Pingping Yang,Jiale Xie
In the zinc ion capacitors (ZICs), Zn2+ storage is limited by the common carbon-based cathodes due to the sluggish kinetics of zinc ion diffusion. Porous carbon derived from metal–organic frameworks (MOFs) is a good candidate with the abundant micropores and mesopores. By using a one-step template-free solvothermal method, double-shell hollow Zn-MOF microspheres were directly synthesized, eliminating the traditional multistep activation process using KOH/HCl. During the carbonization procedure, urea has been used to provide an additional carbon source and activate the derived carbon, which is due to urea pyrolysis products, such as NH3, cyanuric acid, and ammelide. The derived porous carbon (U-MPC) exhibits a high specific surface area of 1695.66 m2 g–1. The ZIC device assembled with U-MPC as the cathode has a high specific capacitance of 199.0 F g–1 and a high energy density of 70.8 Wh kg–1 at 0.1 A g–1. The Zn//U-MPC ZICs demonstrate a good antiself-discharge performance, with a low self-discharge rate of only 3.1 mV h–1 over 300 h, and can maintain 59.7% of the initial capacitance. Moreover, the ZICs exhibit good cyclic stability, showing a slight decrease after 20,000 cycles at 5 A g–1, and the Coulombic efficiency is 90%.
✓ 已验证
产品关联
DOI
高影响因子
危险品化学品经营许可证（带存储）